Cp4.1LG14g10680 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG14g10680
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Protease inhibitor protein
Location	Cp4.1LG14: 8774200 .. 8774857 (-)
RNA-Seq Expression	Cp4.1LG14g10680
Synteny	Cp4.1LG14g10680

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CATTATAAGCTAAGTATTTCGATATAATCAACCAAAGCATTCAACAAGATGTCGGACATCTGTCCTCCCGGTAAGTTCTTCCTATCATATAAATCTCGATTTCAAAACCGTTCAATTGAACGAAGGATTTAGTTTATGATCTATAATGCATAAAAATTTGTCTCTTTCGTTTAAAATTGTTGTACAATGATTCTTCGGCCTAATTTACTTTATTATAATTTTGTCACCTCTCAAAAAAATTTAGATTTTTTCTATATATTAAAAGATTTGGCACGAAAAATGAGATTTTCAAAACTCTTATATTTATATTTGGGCAAATATCTTATTGGTTCGTTCATTTGCAGTAATTTGATTGTGAGTTATGTGCATTACAAAAAATTAACAGGAAAAAAGGAATGGCCAGAACTGGTGGGTATAGAATATTCTATCGCTGCTAGGATAATAGAAAGGGAGAATCCTCATGTCAAAGCACAAAAGATTATCGCGGGGAGTCCGAGAATTATGAATTACAATCCTGGACGAGTTTGGGTTGATGTCAATGTTGAGAATAAGTGTGTTTTAATTCCTAAAGCAGGTTAAGGGATGTAATATGATAAAATATATGAAATTACTAATAAAGTAGTGTGTGACTACCGTTTTAATTATTGAATTGTTCCAA

mRNA sequence

CATTATAAGCTAAGTATTTCGATATAATCAACCAAAGCATTCAACAAGATGTCGGACATCTGTCCTCCCGGAAAAAAGGAATGGCCAGAACTGGTGGGTATAGAATATTCTATCGCTGCTAGGATAATAGAAAGGGAGAATCCTCATGTCAAAGCACAAAAGATTATCGCGGGGAGTCCGAGAATTATGAATTACAATCCTGGACGAGTTTGGGTTGATGTCAATGTTGAGAATAAGTGTGTTTTAATTCCTAAAGCAGGTTAAGGGATGTAATATGATAAAATATATGAAATTACTAATAAAGTAGTGTGTGACTACCGTTTTAATTATTGAATTGTTCCAA

Coding sequence (CDS)

ATGTCGGACATCTGTCCTCCCGGAAAAAAGGAATGGCCAGAACTGGTGGGTATAGAATATTCTATCGCTGCTAGGATAATAGAAAGGGAGAATCCTCATGTCAAAGCACAAAAGATTATCGCGGGGAGTCCGAGAATTATGAATTACAATCCTGGACGAGTTTGGGTTGATGTCAATGTTGAGAATAAGTGTGTTTTAATTCCTAAAGCAGGTTAA

Protein sequence

MSDICPPGKKEWPELVGIEYSIAARIIERENPHVKAQKIIAGSPRIMNYNPGRVWVDVNVENKCVLIPKAG

Homology

[Close]

BLAST of Cp4.1LG14g10680 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 1.0e-07
Identity = 28/65 (43.08%), Postives = 38/65 (58.46%), Query Frame = 0


Query: 7  PGKKEWPELVGIEYSIAARIIERENPHVKAQKIIAGSPRIMNYNPGRVWVDVNVENKCVL 66
          PGK  WP LVG+  S+A  IIER+NP+VKA  +  G+P   ++   RV + VN     V 
Sbjct: 4  PGKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVVS 63

Query: 67 IPKAG 72
           P+ G
Sbjct: 64 PPRIG 68

[Close]

BLAST of Cp4.1LG14g10680 vs. ExPASy Swiss-Prot
Match: P82381 (Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 3.2e-06
Identity = 26/62 (41.94%), Postives = 34/62 (54.84%), Query Frame = 0


Query: 7  PGKKEWPELVGIEYSIAARIIERENPHVKAQKIIAGSPRIMNYNPGRVWVDVNVENKCVL 66
          PGK  WPELVG   ++AA  +EREN +V A  +  GS    ++   RVWV VN       
Sbjct: 5  PGKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDHGVVTS 64

Query: 67 IP 69
          +P
Sbjct: 65 VP 66

[Close]

BLAST of Cp4.1LG14g10680 vs. ExPASy Swiss-Prot
Match: Q02214 (Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 1.2e-05
Identity = 27/69 (39.13%), Postives = 39/69 (56.52%), Query Frame = 0


Query: 5  CPPGKKE-WPELVGIEYSIAARIIERENPHV-KAQKIIAGSPRIMNYNPGRVWVDVNVEN 64
          CP  KKE WPEL+G+    A  II++EN  +     ++ GSP   ++   RV + VNV +
Sbjct: 26 CPGNKKETWPELIGVPAKFAREIIQKENSKLTNVPSVLNGSPVTKDFRCERVRLFVNVLD 85

Query: 65 KCVLIPKAG 72
            V IP+ G
Sbjct: 86 FVVQIPRVG 94

[Close]

BLAST of Cp4.1LG14g10680 vs. ExPASy Swiss-Prot
Match: P80211 (Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 1.6e-05
Identity = 28/63 (44.44%), Postives = 36/63 (57.14%), Query Frame = 0


Query: 7  PGKKEWPELVGIEYSIAARIIERENPHVKAQKIIAGSPRIMNYNPGRVWVDVNVENKCVL 66
          PGK+EWPELVG     AA IIERENP+V++      S    ++   RVWV V+     V 
Sbjct: 5  PGKQEWPELVGEYGYKAAAIIERENPNVRSIVKHERSGFTKDFRCDRVWVVVDSTGVVVR 64

Query: 67 IPK 70
           P+
Sbjct: 65 TPR 67

[Close]

BLAST of Cp4.1LG14g10680 vs. ExPASy Swiss-Prot
Match: Q6XNP7 (Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2)

HSP 1 Score: 49.7 bits (117), Expect = 1.6e-05
Identity = 29/61 (47.54%), Postives = 37/61 (60.66%), Query Frame = 0


Query: 1  MSDICPPGKKEWPELVGIEYSIAARIIERENPHVKAQKIIAGSP--RIMNYNPGRVWVDV 60
          M+  CP  K  WPELVG    IAA II+ EN +VKA  +  G P  + +N+N  RV+VD 
Sbjct: 1  MASQCPV-KNSWPELVGTNGDIAAGIIQTENANVKAIVVKEGLPITQDLNFNRVRVFVDE 60

[Close]

BLAST of Cp4.1LG14g10680 vs. NCBI nr
Match: KAA0049286.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17272.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 89.4 bits (220), Expect = 1.59e-21
Identity = 41/63 (65.08%), Postives = 49/63 (77.78%), Query Frame = 0


Query: 9  KKEWPELVGIEYSIAARIIERENPHVKAQKIIAGSPRIMNYNPGRVWVDVNVENKCVLIP 68
          K  WPELV +E+S AARIIE+ENP VKA KI+A SPRI N++P RVWVD NVE+K V +P
Sbjct: 9  KTRWPELVFVEFSTAARIIEKENPDVKAIKILADSPRIENFDPSRVWVDCNVEDKVVKVP 68

Query: 69 KAG 71
            G
Sbjct: 69 SVG 71

[Close]

BLAST of Cp4.1LG14g10680 vs. NCBI nr
Match: KGN56897.1 (hypothetical protein Csa_010510 [Cucumis sativus])

HSP 1 Score: 89.0 bits (219), Expect = 2.25e-21
Identity = 41/71 (57.75%), Postives = 54/71 (76.06%), Query Frame = 0


Query: 1  MSDICPPGKKEWPELVGIEYSIAARIIERENPHVKAQKIIAGSPRIMNYNPGRVWVDVNV 60
          M++   P K EWPELV ++YSIAA IIE+ENP V+A KI++G+PRI+N++  RVWVD+NV
Sbjct: 1  MAEESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDINV 60

Query: 61 ENKCVLIPKAG 71
          E   V  PK G
Sbjct: 61 EEVVVKKPKVG 71

[Close]

BLAST of Cp4.1LG14g10680 vs. NCBI nr
Match: KAA0049288.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17270.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 86.7 bits (213), Expect = 1.01e-19
Identity = 41/67 (61.19%), Postives = 52/67 (77.61%), Query Frame = 0


Query: 3  DICPPGKKEWPELVGIEYSIAARIIERENPHVKAQKIIAGSPRIMNYNPGRVWVDVNVEN 62
          D   P K +WPELV I+YSIAA IIERENP V+A KI++G+PRI+N++  RVW DVNVE+
Sbjct: 4  DQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVNVED 63

Query: 63 KCVLIPK 69
            V +PK
Sbjct: 64 VIVKMPK 70

[Close]

BLAST of Cp4.1LG14g10680 vs. NCBI nr
Match: KAE8650339.1 (hypothetical protein Csa_009729 [Cucumis sativus])

HSP 1 Score: 84.3 bits (207), Expect = 3.44e-18
Identity = 39/61 (63.93%), Postives = 48/61 (78.69%), Query Frame = 0


Query: 9  KKEWPELVGIEYSIAARIIERENPHVKAQKIIAGSPRIMNYNPGRVWVDVNVENKCVLIP 68
          K  WPELVG+++S AARIIE ENP+VKA KI+ GSP I+NY+P RVWV  N E+K V IP
Sbjct: 9  KSIWPELVGVDFSAAARIIETENPNVKATKILIGSPVILNYDPTRVWVICNTEDKVVDIP 68

[Close]

BLAST of Cp4.1LG14g10680 vs. NCBI nr
Match: KAA0049287.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17271.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 80.5 bits (197), Expect = 5.22e-18
Identity = 39/69 (56.52%), Postives = 48/69 (69.57%), Query Frame = 0


Query: 3  DICPPGKKEWPELVGIEYSIAARIIERENPHVKAQKIIAGSPRIMNYNPGRVWVDVNVEN 62
          D   P K +WPELV ++YS AA IIE+ENP VKA KI++G  RI N++  RVW DVNVE+
Sbjct: 4  DQSAPRKTQWPELVFVKYSTAAAIIEKENPDVKAVKILSGRFRIQNFDTKRVWCDVNVED 63

Query: 63 KCVLIPKAG 71
            V  PK G
Sbjct: 64 VVVKTPKVG 72

[Close]

BLAST of Cp4.1LG14g10680 vs. ExPASy TrEMBL
Match: A0A5A7U4U0 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001250 PE=3 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 7.68e-22
Identity = 41/63 (65.08%), Postives = 49/63 (77.78%), Query Frame = 0


Query: 9  KKEWPELVGIEYSIAARIIERENPHVKAQKIIAGSPRIMNYNPGRVWVDVNVENKCVLIP 68
          K  WPELV +E+S AARIIE+ENP VKA KI+A SPRI N++P RVWVD NVE+K V +P
Sbjct: 9  KTRWPELVFVEFSTAARIIEKENPDVKAIKILADSPRIENFDPSRVWVDCNVEDKVVKVP 68

Query: 69 KAG 71
            G
Sbjct: 69 SVG 71

[Close]

BLAST of Cp4.1LG14g10680 vs. ExPASy TrEMBL
Match: A0A0A0L8E6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142910 PE=3 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.09e-21
Identity = 41/71 (57.75%), Postives = 54/71 (76.06%), Query Frame = 0


Query: 1  MSDICPPGKKEWPELVGIEYSIAARIIERENPHVKAQKIIAGSPRIMNYNPGRVWVDVNV 60
          M++   P K EWPELV ++YSIAA IIE+ENP V+A KI++G+PRI+N++  RVWVD+NV
Sbjct: 1  MAEESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDINV 60

Query: 61 ENKCVLIPKAG 71
          E   V  PK G
Sbjct: 61 EEVVVKKPKVG 71

[Close]

BLAST of Cp4.1LG14g10680 vs. ExPASy TrEMBL
Match: A0A0A0L9Z4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142410 PE=3 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 8.97e-21
Identity = 40/63 (63.49%), Postives = 49/63 (77.78%), Query Frame = 0


Query: 9  KKEWPELVGIEYSIAARIIERENPHVKAQKIIAGSPRIMNYNPGRVWVDVNVENKCVLIP 68
          K  WPELVG+++S AARIIE ENP+VKA KI+ GSP I+NY+P RVWV  N E+K V IP
Sbjct: 9  KSIWPELVGVDFSAAARIIETENPNVKATKILIGSPVILNYDPTRVWVICNTEDKVVDIP 68

Query: 69 KAG 71
          + G
Sbjct: 69 RVG 71

[Close]

BLAST of Cp4.1LG14g10680 vs. ExPASy TrEMBL
Match: A0A5A7U228 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001230 PE=3 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 4.89e-20
Identity = 41/67 (61.19%), Postives = 52/67 (77.61%), Query Frame = 0


Query: 3  DICPPGKKEWPELVGIEYSIAARIIERENPHVKAQKIIAGSPRIMNYNPGRVWVDVNVEN 62
          D   P K +WPELV I+YSIAA IIERENP V+A KI++G+PRI+N++  RVW DVNVE+
Sbjct: 4  DQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVNVED 63

Query: 63 KCVLIPK 69
            V +PK
Sbjct: 64 VIVKMPK 70

[Close]

BLAST of Cp4.1LG14g10680 vs. ExPASy TrEMBL
Match: A0A5A7U4L9 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001240 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.53e-18
Identity = 39/69 (56.52%), Postives = 48/69 (69.57%), Query Frame = 0


Query: 3  DICPPGKKEWPELVGIEYSIAARIIERENPHVKAQKIIAGSPRIMNYNPGRVWVDVNVEN 62
          D   P K +WPELV ++YS AA IIE+ENP VKA KI++G  RI N++  RVW DVNVE+
Sbjct: 4  DQSAPRKTQWPELVFVKYSTAAAIIEKENPDVKAVKILSGRFRIQNFDTKRVWCDVNVED 63

Query: 63 KCVLIPKAG 71
            V  PK G
Sbjct: 64 VVVKTPKVG 72

[Close]

BLAST of Cp4.1LG14g10680 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 55.5 bits (132), Expect = 2.1e-08
Identity = 32/71 (45.07%), Postives = 38/71 (53.52%), Query Frame = 0


Query: 1  MSDICPPGKKEWPELVGIEYSIAARIIERENPHVKAQKIIAGSPRIMNYNPGRVWVDVNV 60
          MS  C P K  WPEL G     AA +IERENP V A  I+ GSP   ++   RV V V+ 
Sbjct: 1  MSTEC-PRKNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDG 60

Query: 61 ENKCVLIPKAG 72
              V  PK+G
Sbjct: 61 NRIVVKTPKSG 70

[Close]

BLAST of Cp4.1LG14g10680 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 51.6 bits (122), Expect = 3.0e-07
Identity = 31/72 (43.06%), Postives = 40/72 (55.56%), Query Frame = 0


Query: 1  MSDIC-PPGKKEWPELVGIEYSIAARIIERENPHVKAQKIIAGSPRIMNYNPGRVWVDVN 60
          M+ IC  PGK  WPEL+G +   A  +IERENP +KA  I+ G+     +   RV+V VN
Sbjct: 28 MASICEDPGKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVN 87

Query: 61 VENKCVLIPKAG 72
               V IP  G
Sbjct: 88 DCGIVVQIPIIG 99

[Close]

BLAST of Cp4.1LG14g10680 vs. TAIR 10
Match: AT3G46860.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 48.1 bits (113), Expect = 3.3e-06
Identity = 28/70 (40.00%), Postives = 36/70 (51.43%), Query Frame = 0


Query: 3  DICPPGKK-EWPELVGIEYSIAARIIERENPHVKAQKIIAGSPRIMNYNPGRVWVDVNVE 62
          + C  GKK  WPELVG    +A   IERENP+V A  +  G  RI N+   RV+V +   
Sbjct: 16 ECCAIGKKYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENFCCNRVFVYLGSN 75

Query: 63 NKCVLIPKAG 72
           +    P  G
Sbjct: 76 GQVADAPMIG 85

The following BLAST results are available for this feature:

BLAST of Cp4.1LG14g10680 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P19873	1.0e-07	43.08	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
P82381	3.2e-06	41.94	Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1	[more]
Q02214	1.2e-05	39.13	Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1	[more]
P80211	1.6e-05	44.44	Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1	[more]
Q6XNP7	1.6e-05	47.54	Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2	[more]

BLAST of Cp4.1LG14g10680 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0049286.1	1.59e-21	65.08	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17272.1 Protease inhib...	[more]
KGN56897.1	2.25e-21	57.75	hypothetical protein Csa_010510 [Cucumis sativus]	[more]
KAA0049288.1	1.01e-19	61.19	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17270.1 Protease inhib...	[more]
KAE8650339.1	3.44e-18	63.93	hypothetical protein Csa_009729 [Cucumis sativus]	[more]
KAA0049287.1	5.22e-18	56.52	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17271.1 Protease inhib...	[more]

BLAST of Cp4.1LG14g10680 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U4U0	7.68e-22	65.08	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A0A0L8E6	1.09e-21	57.75	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142910 PE=3 SV=1	[more]
A0A0A0L9Z4	8.97e-21	63.49	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142410 PE=3 SV=1	[more]
A0A5A7U228	4.89e-20	61.19	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A5A7U4L9	2.53e-18	56.52	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]

BLAST of Cp4.1LG14g10680 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	2.1e-08	45.07	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	3.0e-07	43.06	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT3G46860.1	3.3e-06	40.00	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 2..70 e-value: 4.8E-19 score: 70.4
None	No IPR available	PANTHER	PTHR33091:SF62	PROTEASE INHIBITOR PROTEIN	coord: 3..70
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 9..71 e-value: 7.0E-15 score: 55.4
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 3..70
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 5..71

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG14g10680.1	Cp4.1LG14g10680.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity