Cp4.1LG14g10490 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG14g10490
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionendochitinase-like
LocationCp4.1LG14: 8806489 .. 8808704 (+)
RNA-Seq ExpressionCp4.1LG14g10490
SyntenyCp4.1LG14g10490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGATAAAGTTCATCAATTTCATGGTAAAAATGGATGTTTTCCTTCAAAATTTTCAACATCGGTACCCTTTCAAACGGTGCGTTTCGATGTTTCCTTTCATTTTTCCCAATACTTCGAAACGAAACCGGCATCATTGTTATCGGATAAGTCAACAGACAAATCGCGGAGCGGCGATCGATGAGAAAAATCTACCGTTCTTTCGTAGCTCTAGCCTTCGTTCTCTTCACATTGGCGACGTCATGGGCGCAGCCGTGCGGCGGCGGCGGAAGAGAACCGGAGAACGCGATGTGTCCAAAAGGCCTCTGCAGCGCCGGGTCGGGGTTCTGCAGATCCGGATTTGTGCGGCGATGTGGTTCTGATCTAACATTTGAAGGCCCTATCGGCGAAATCGGCGGCGTAATTAGCGCCTCGGATTTTGACGAAATGCTAAAACATCGAAACGATCGGAATTGCAAGAGCAATGGATTTTACAGCTACGAGGCTTTTGCGGTTGCGGCGGCGGCGTTCGACGGCTTTGGCACCACCGGGAATCTCAACACCAGGAAGAGGGAGGTCGCCGCTTTCCTCGCTCAAACATCTCACGAGACCAAAGGTTAATCATTCACTTCTAATTTACTGACTATTTTCTCTTATAATTATGGTTAAATTATAAGTTTTTTTTTTTACTTCACTATGAAAAATTTAGAAGGAAATTTGATGGATTGACCAAAAGTTTGGGTCGAGATGAAATATGACCTCCTTTCAAATTACGTGCTCTCCTGATAAACGTATTTTCATATAATCCCTGATGACAACACAGAGTTAAACAATCTTAATTATATAAAGTTTTTGGGTCAATTGGTCAAGTTTTACGAGATTGTTCGCGGAAATATTTTGATATGGAATCAACTCAAGAGAAAGTTTTTGAAATAGATTACCTTGATGATCAAGATTAGAAAATGCTTGTTTTTAAACTCTGTGATTCGAATCACTCCACGGGTTTGATCAAATACCACTGAGATGACTATAACATGTCAGTTTTAAATATAAGAATTTGCAAAGTAAAAACTTAGTTATCAACAAAGCTAATATAGAAATTCTATTCTTTTCAATTCCACGATGTGATGTTAGAGCAATAACATGAAAGACTATTTATAATCGGGAAAATAAACCTTATAATAATGGGTCAATACCATATTGACCGCTAACTAATTATAACTTATAATTTAAAGACAAATCTCTAAAATTTAAAAAATACAATAACACTGACAATTTTCTAAAACTTAAAAAATACAATAACACTTTTTTAAATCTAAATCATCCATCCATGACTTAATTAAGACACCTTTCTTACTCTATTTAATTGATCTATTTAATTGATGTTGAATTTGTTTGATTTATTAATTTTTAAATGTGTTTAATAGGCCCTCATCGTTGATGTTAAGTTAAAAGAAATGGAATGTTTGTATAGGAGGATGGCAAACTGCACCGGATGGACCATATGCATGGGGCTATTGCTATATTAATTCAACCATCAAAGATGCTTCTTGTAGTCCTTCTACCAACTGGCCTTGTGCATCTGGGAAGCAATATTATGGCCGAGGTCCCTTCCAAATCACCTAGTAAGTTCGATGAAATTCTACTTTTAACCAAATATTGATTTGTATCATTGAAAGATGGCTTTTGGTTGATTAGTAACTTCAACTATGGGGCTGCTGGAAGGGCAATAGGGGTGGACCTGATAAACAATCCTGATCTAGTGGCTACCAATCCAACCATATCATTCAAGACCGCCTTGTGGTTTTGGATGACTCCTCAAGGAAACAAACCATCGTGTCATGATGTTATGGCGGGTAAATGGGTACCATCCAATGAAGATATATCAGCTAAAAGATTGCCTGGATATGGTCTAACCATCAACTTAATCAATGCTAGTGAATGTGTTGATGGTACCGATGATCGAGCTGCAGATAGGATTGGTTTCTACAAAAGGTATTGTGACATATTGAATATCCAATATGGAGACAACTTGGATTGTGTAGGTCAACAGCCCTTCTAATGAAGAATGATTAGTAAGAAGTTGTAAGAAATGTAGGGAGGCAAAAGTAGATAGTAATTTGGAGATTATGATCCCTTCGATACTCGGTAATTTTCAATAGTTGTAAACATTTTACTAGTAAAGAATATTTTTCTAATTATCAGAATGAAGCGCTCAATGACAATCAGAATTAGATCT

mRNA sequence

TTGATAAAGTTCATCAATTTCATGGTAAAAATGGATGTTTTCCTTCAAAATTTTCAACATCGGTACCCTTTCAAACGGTGCGTTTCGATGTTTCCTTTCATTTTTCCCAATACTTCGAAACGAAACCGGCATCATTGTTATCGGATAAGTCAACAGACAAATCGCGGAGCGGCGATCGATGAGAAAAATCTACCGTTCTTTCGTAGCTCTAGCCTTCGTTCTCTTCACATTGGCGACGTCATGGGCGCAGCCGTGCGGCGGCGGCGGAAGAGAACCGGAGAACGCGATGTGTCCAAAAGGCCTCTGCAGCGCCGGGTCGGGGTTCTGCAGATCCGGATTTGTGCGGCGATGTGGTTCTGATCTAACATTTGAAGGCCCTATCGGCGAAATCGGCGGCGTAATTAGCGCCTCGGATTTTGACGAAATGCTAAAACATCGAAACGATCGGAATTGCAAGAGCAATGGATTTTACAGCTACGAGGCTTTTGCGGTTGCGGCGGCGGCGTTCGACGGCTTTGGCACCACCGGGAATCTCAACACCAGGAAGAGGGAGGTCGCCGCTTTCCTCGCTCAAACATCTCACGAGACCAAAGGAGGATGGCAAACTGCACCGGATGGACCATATGCATGGGGCTATTGCTATATTAATTCAACCATCAAAGATGCTTCTTGTAGTCCTTCTACCAACTGGCCTTGTGCATCTGGGAAGCAATATTATGGCCGAGGTCCCTTCCAAATCACCTATAACTTCAACTATGGGGCTGCTGGAAGGGCAATAGGGGTGGACCTGATAAACAATCCTGATCTAGTGGCTACCAATCCAACCATATCATTCAAGACCGCCTTGTGGTTTTGGATGACTCCTCAAGGAAACAAACCATCGTGTCATGATGTTATGGCGGGTAAATGGGTACCATCCAATGAAGATATATCAGCTAAAAGATTGCCTGGATATGGTCTAACCATCAACTTAATCAATGCTAGTGAATGTGTTGATGGTACCGATGATCGAGCTGCAGATAGGATTGGTTTCTACAAAAGGTATTGTGACATATTGAATATCCAATATGGAGACAACTTGGATTGTGTAGGTCAACAGCCCTTCTAATGAAGAATGATTAGTAAGAAGTTGTAAGAAATGTAGGGAGGCAAAAGTAGATAGTAATTTGGAGATTATGATCCCTTCGATACTCGGTAATTTTCAATAGTTGTAAACATTTTACTAGTAAAGAATATTTTTCTAATTATCAGAATGAAGCGCTCAATGACAATCAGAATTAGATCT

Coding sequence (CDS)

ATGAGAAAAATCTACCGTTCTTTCGTAGCTCTAGCCTTCGTTCTCTTCACATTGGCGACGTCATGGGCGCAGCCGTGCGGCGGCGGCGGAAGAGAACCGGAGAACGCGATGTGTCCAAAAGGCCTCTGCAGCGCCGGGTCGGGGTTCTGCAGATCCGGATTTGTGCGGCGATGTGGTTCTGATCTAACATTTGAAGGCCCTATCGGCGAAATCGGCGGCGTAATTAGCGCCTCGGATTTTGACGAAATGCTAAAACATCGAAACGATCGGAATTGCAAGAGCAATGGATTTTACAGCTACGAGGCTTTTGCGGTTGCGGCGGCGGCGTTCGACGGCTTTGGCACCACCGGGAATCTCAACACCAGGAAGAGGGAGGTCGCCGCTTTCCTCGCTCAAACATCTCACGAGACCAAAGGAGGATGGCAAACTGCACCGGATGGACCATATGCATGGGGCTATTGCTATATTAATTCAACCATCAAAGATGCTTCTTGTAGTCCTTCTACCAACTGGCCTTGTGCATCTGGGAAGCAATATTATGGCCGAGGTCCCTTCCAAATCACCTATAACTTCAACTATGGGGCTGCTGGAAGGGCAATAGGGGTGGACCTGATAAACAATCCTGATCTAGTGGCTACCAATCCAACCATATCATTCAAGACCGCCTTGTGGTTTTGGATGACTCCTCAAGGAAACAAACCATCGTGTCATGATGTTATGGCGGGTAAATGGGTACCATCCAATGAAGATATATCAGCTAAAAGATTGCCTGGATATGGTCTAACCATCAACTTAATCAATGCTAGTGAATGTGTTGATGGTACCGATGATCGAGCTGCAGATAGGATTGGTTTCTACAAAAGGTATTGTGACATATTGAATATCCAATATGGAGACAACTTGGATTGTGTAGGTCAACAGCCCTTCTAA

Protein sequence

MRKIYRSFVALAFVLFTLATSWAQPCGGGGREPENAMCPKGLCSAGSGFCRSGFVRRCGSDLTFEGPIGEIGGVISASDFDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFDGFGTTGNLNTRKREVAAFLAQTSHETKGGWQTAPDGPYAWGYCYINSTIKDASCSPSTNWPCASGKQYYGRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDVMAGKWVPSNEDISAKRLPGYGLTINLINASECVDGTDDRAADRIGFYKRYCDILNIQYGDNLDCVGQQPF
Homology
BLAST of Cp4.1LG14g10490 vs. ExPASy Swiss-Prot
Match: P51613 (Basic endochitinase OS=Vitis vinifera OX=29760 GN=CHIT1B PE=2 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 4.0e-101
Identity = 179/306 (58.50%), Postives = 215/306 (70.26%), Query Frame = 0

Query: 11  LAFVLFTL-ATSWAQPCGG--GGRE-PENAMCPK-GLCSAGSGFCRSGFVRRCGSDLTFE 70
           +AF L +L     A+ CGG  GGR  P  A C K G C   + +C SG   +C S     
Sbjct: 7   VAFCLLSLILVGSAEQCGGQAGGRVCPGGACCSKFGWCGNTADYCGSGCQSQCSS----- 66

Query: 71  GPIGEIGGVISASDFDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFDGFGTTGNLNTRKRE 130
              G+IG +I+ S F++MLKHRN+ +C   GFY+Y+AF  AA AF GFGTTG+  TRKRE
Sbjct: 67  --TGDIGQLITRSMFNDMLKHRNEGSCPGKGFYTYDAFIAAAKAFPGFGTTGDTTTRKRE 126

Query: 131 VAAFLAQTSHETKGGWQTAPDGPYAWGYCYINST-IKDASCSPSTNWPCASGKQYYGRGP 190
           +AAFLAQTSHET GGW +APDGPYAWGYCY+       A C PS  WPCA+G++YYGRGP
Sbjct: 127 IAAFLAQTSHETTGGWASAPDGPYAWGYCYLREQGSPGAYCVPSAQWPCAAGRKYYGRGP 186

Query: 191 FQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDVMAGKW 250
            QI+YN+NYG AG+AIGVDL+NNPDLVAT+  ISFKTA WFWMTPQ  KPSCH+V+ G W
Sbjct: 187 IQISYNYNYGQAGKAIGVDLVNNPDLVATDAVISFKTAFWFWMTPQSPKPSCHNVITGGW 246

Query: 251 VPSNEDISAKRLPGYGLTINLINAS-ECVDGTDDRAADRIGFYKRYCDILNIQYGDNLDC 310
            PS  D SA RLPG+G+  N+IN   EC  G   +  DRIGFYKRYCDIL + YG+NLDC
Sbjct: 247 TPSGADRSAGRLPGFGVITNIINGGVECGKGVVPQVQDRIGFYKRYCDILRVSYGNNLDC 305

BLAST of Cp4.1LG14g10490 vs. ExPASy Swiss-Prot
Match: P05315 (Endochitinase OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 8.9e-101
Identity = 174/310 (56.13%), Postives = 220/310 (70.97%), Query Frame = 0

Query: 11  LAFVLFTL----ATSWAQPCG--GGGREPENAMCPK--GLCSAGSGFCRSGFVR-RCGSD 70
           +A++LF+L    + + AQ CG  GGG+   +  C    G C   + +C SG  + +C   
Sbjct: 10  VAYLLFSLLVLVSAALAQNCGSQGGGKACASGQCCSKFGWCGNTNDYCGSGNCQSQCPGG 69

Query: 71  LTFEGPIGEIGGVISASDFDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFDGFGTTGNLNT 130
               GP G++G  IS S FD+MLKHRN+ +C+   FYSY AF  AA +F GFGT+G++N 
Sbjct: 70  GPGPGPGGDLGSAISNSMFDQMLKHRNENSCQGKNFYSYNAFINAARSFPGFGTSGDINA 129

Query: 131 RKREVAAFLAQTSHETKGGWQTAPDGPYAWGYCYINSTIKDAS-CSPSTNWPCASGKQYY 190
           RKRE+AAF AQTSHET GGW +APDGPYAWGYC++         C PS+ WPCA G++Y+
Sbjct: 130 RKREIAAFFAQTSHETTGGWASAPDGPYAWGYCFLRERGNPGDYCPPSSQWPCAPGRKYF 189

Query: 191 GRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDVM 250
           GRGP QI++N+NYG  GRAIGVDL+NNPDLVAT+P ISFKTALWFWMTPQ  KPSCHDV+
Sbjct: 190 GRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKTALWFWMTPQSPKPSCHDVI 249

Query: 251 AGKWVPSNEDISAKRLPGYGLTINLINAS-ECVDGTDDRAADRIGFYKRYCDILNIQYGD 310
            G+W PS+ D +A RLPG+G+  N+IN   EC  GTD+R  DRIGFY+RYC IL +  GD
Sbjct: 250 IGRWNPSSADRAANRLPGFGVITNIINGGLECGRGTDNRVQDRIGFYRRYCSILGVTPGD 309

BLAST of Cp4.1LG14g10490 vs. ExPASy Swiss-Prot
Match: Q09023 (Endochitinase CH25 OS=Brassica napus OX=3708 PE=2 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 3.4e-100
Identity = 171/311 (54.98%), Postives = 217/311 (69.77%), Query Frame = 0

Query: 11  LAFVLFT--LATSWAQPCGGGGREPENAMCPKGLCSAGSGFC--------RSGFVRRCGS 70
           L F++F+  L+ S A+ C   GR+   A+CP GLC +  G+C        + G   +CG 
Sbjct: 6   LLFLIFSFLLSFSLAEQC---GRQAGGALCPNGLCCSEFGWCGDTEAYCKQPGCQSQCGG 65

Query: 71  DLTFEGPIGEIGGVISASDFDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFDGFGTTGNLN 130
             T  GP G++ G+IS S FD+MLKHRND  C + GFY+Y+AF  AA +F GFGTTG+  
Sbjct: 66  --TPPGPTGDLSGIISRSQFDDMLKHRNDNACPARGFYTYDAFINAAKSFPGFGTTGDTA 125

Query: 131 TRKREVAAFLAQTSHETKGGWQTAPDGPYAWGYCYINSTIKDAS-CSPSTNWPCASGKQY 190
           TRK+E+AAF  QTSHET GGW TAPDGPY+WGYC+       ++ CSPS  WPCASGK Y
Sbjct: 126 TRKKEIAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPSSNYCSPSAEWPCASGKSY 185

Query: 191 YGRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDV 250
           YGRGP Q+++N+NYG  GRAIG DL+NNPDLV+ +P I+FK A+WFWMTPQ  KPSCH V
Sbjct: 186 YGRGPMQLSWNYNYGQCGRAIGSDLLNNPDLVSNDPVIAFKAAIWFWMTPQSPKPSCHAV 245

Query: 251 MAGKWVPSNEDISAKRLPGYGLTINLINAS-ECVDGTDDRAADRIGFYKRYCDILNIQYG 310
           + G+W PS+ D +A R+PGYG+  N+IN   EC  G D R ADRIGFY+RYC+IL +  G
Sbjct: 246 IVGQWQPSDADRAAGRVPGYGVITNIINGGLECGRGQDARVADRIGFYQRYCNILGVNPG 305

BLAST of Cp4.1LG14g10490 vs. ExPASy Swiss-Prot
Match: P24091 (Endochitinase B OS=Nicotiana tabacum OX=4097 GN=CHN50 PE=1 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 9.8e-100
Identity = 176/313 (56.23%), Postives = 218/313 (69.65%), Query Frame = 0

Query: 6   RSFVALAFVLFT--LATSWAQPCG---GGGREPENAMCPK-GLCSAGSGFCRSGFVR-RC 65
           R F AL+ +LF+  L ++ A+ CG   GG R      C K G C   + +C  G  + +C
Sbjct: 4   REFTALSSLLFSLLLLSASAEQCGSQAGGARCASGLCCSKFGWCGNTNDYCGPGNCQSQC 63

Query: 66  GSDLTFEGPIGEIGGVISASDFDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFDGFGTTGN 125
               T  G  G++G +IS+S FD+MLKHRND  C+  GFYSY AF  AA +F GFGT+G+
Sbjct: 64  PGGPTPPGG-GDLGSIISSSMFDQMLKHRNDNACQGKGFYSYNAFINAARSFPGFGTSGD 123

Query: 126 LNTRKREVAAFLAQTSHETKGGWQTAPDGPYAWGYCYINSTIKDAS-CSPSTNWPCASGK 185
              RKRE+AAF AQTSHET GGW TAPDGPYAWGYC++         C+PS  WPCA G+
Sbjct: 124 TTARKREIAAFFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPGDYCTPSGQWPCAPGR 183

Query: 186 QYYGRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCH 245
           +Y+GRGP QI++N+NYG  GRAIGVDL+NNPDLVAT+P ISFK+ALWFWMTPQ  KPSCH
Sbjct: 184 KYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKSALWFWMTPQSPKPSCH 243

Query: 246 DVMAGKWVPSNEDISAKRLPGYGLTINLINAS-ECVDGTDDRAADRIGFYKRYCDILNIQ 305
           DV+ G+W PS+ D +A RLPG+G+  N+IN   EC  GTD R  DRIGFY+RYC IL + 
Sbjct: 244 DVIIGRWQPSSADRAANRLPGFGVITNIINGGLECGRGTDSRVQDRIGFYRRYCSILGVS 303

Query: 306 YGDNLDCVGQQPF 310
            GDNLDC  Q+ F
Sbjct: 304 PGDNLDCGNQRSF 315

BLAST of Cp4.1LG14g10490 vs. ExPASy Swiss-Prot
Match: P21226 (Endochitinase A2 OS=Pisum sativum OX=3888 GN=CHI2 PE=1 SV=2)

HSP 1 Score: 364.0 bits (933), Expect = 1.7e-99
Identity = 171/305 (56.07%), Postives = 204/305 (66.89%), Query Frame = 0

Query: 9   VALAFVLFTLATSWAQPCGGGGREPENAMCPKGLCSAGSGFCRSGFV---RRCGSDLTFE 68
           + +  VLF ++   A+ C   G +   A+CP GLC +  G+C S        C S  +  
Sbjct: 6   IPILLVLFIVSCCSAEQC---GTQAGGALCPGGLCCSKFGWCGSTSEYCGDGCQSQCSGS 65

Query: 69  GPIGEIGGVISASDFDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFDGFGTTGNLNTRKRE 128
              G +  +IS   F+ MLKHRND  C+   FY+Y+AF  AA AF  F   G+  T+KRE
Sbjct: 66  SGGGTLSSLISGDTFNNMLKHRNDNACQGKPFYTYDAFLSAAKAFPNFANKGDTATKKRE 125

Query: 129 VAAFLAQTSHETKGGWQTAPDGPYAWGYCYINSTIKDASCSPSTNWPCASGKQYYGRGPF 188
           +AAFL QTSHET GGW TAPDGPYAWGYC++        C  S+ +PCASGKQYYGRGP 
Sbjct: 126 IAAFLGQTSHETTGGWPTAPDGPYAWGYCFLREQNPSTYCQASSEFPCASGKQYYGRGPI 185

Query: 189 QITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDVMAGKWV 248
           QI++N+NYG  GRAIGVDL+NNPDLVAT+P ISFKTALWFWMTPQ  KPSCHDV+ G W 
Sbjct: 186 QISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTALWFWMTPQSPKPSCHDVITGGWT 245

Query: 249 PSNEDISAKRLPGYGLTINLINAS-ECVDGTDDRAADRIGFYKRYCDILNIQYGDNLDCV 308
           PS+ D +A RLPGYG   N+IN   EC  G D R  DRIGFYKRYCDI  I YGDNLDC 
Sbjct: 246 PSSADRAAGRLPGYGTVTNIINGGLECGRGQDSRVQDRIGFYKRYCDIFGIGYGDNLDCY 305

Query: 309 GQQPF 310
            Q+PF
Sbjct: 306 SQRPF 307

BLAST of Cp4.1LG14g10490 vs. NCBI nr
Match: XP_023553171.1 (basic endochitinase C-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 663 bits (1710), Expect = 6.13e-241
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0

Query: 1   MRKIYRSFVALAFVLFTLATSWAQPCGGGGREPENAMCPKGLCSAGSGFCRSGFVRRCGS 60
           MRKIYRSFVALAFVLFTLATSWAQPCGGGGREPENAMCPKGLCSAGSGFCRSGFVRRCGS
Sbjct: 1   MRKIYRSFVALAFVLFTLATSWAQPCGGGGREPENAMCPKGLCSAGSGFCRSGFVRRCGS 60

Query: 61  DLTFEGPIGEIGGVISASDFDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFDGFGTTGNLN 120
           DLTFEGPIGEIGGVISASDFDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFDGFGTTGNLN
Sbjct: 61  DLTFEGPIGEIGGVISASDFDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFDGFGTTGNLN 120

Query: 121 TRKREVAAFLAQTSHETKGGWQTAPDGPYAWGYCYINSTIKDASCSPSTNWPCASGKQYY 180
           TRKREVAAFLAQTSHETKGGWQTAPDGPYAWGYCYINSTIKDASCSPSTNWPCASGKQYY
Sbjct: 121 TRKREVAAFLAQTSHETKGGWQTAPDGPYAWGYCYINSTIKDASCSPSTNWPCASGKQYY 180

Query: 181 GRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDVM 240
           GRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDVM
Sbjct: 181 GRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDVM 240

Query: 241 AGKWVPSNEDISAKRLPGYGLTINLINASECVDGTDDRAADRIGFYKRYCDILNIQYGDN 300
           AGKWVPSNEDISAKRLPGYGLTINLINASECVDGTDDRAADRIGFYKRYCDILNIQYGDN
Sbjct: 241 AGKWVPSNEDISAKRLPGYGLTINLINASECVDGTDDRAADRIGFYKRYCDILNIQYGDN 300

Query: 301 LDCVGQQPF 309
           LDCVGQQPF
Sbjct: 301 LDCVGQQPF 309

BLAST of Cp4.1LG14g10490 vs. NCBI nr
Match: XP_022923516.1 (basic endochitinase C-like [Cucurbita moschata])

HSP 1 Score: 625 bits (1613), Expect = 3.38e-226
Identity = 294/309 (95.15%), Postives = 296/309 (95.79%), Query Frame = 0

Query: 1   MRKIYRSFVALAFVLFTLATSWAQPCGGGGREPENAMCPKGLCSAGSGFCRSGFVRRCGS 60
           MRKI   FVALAFVLFT ATSWAQPCGGGG EPENAMCPKGLCSAGSGFCRSGFVRRCGS
Sbjct: 1   MRKI---FVALAFVLFTSATSWAQPCGGGGGEPENAMCPKGLCSAGSGFCRSGFVRRCGS 60

Query: 61  DLTFEGPIGEIGGVISASDFDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFDGFGTTGNLN 120
           DLTF  P GEIG VISASDFDEMLKHRNDR+CKSNGFYSYEAFAVAAAAFDGFGTTGNLN
Sbjct: 61  DLTFGSPTGEIGSVISASDFDEMLKHRNDRSCKSNGFYSYEAFAVAAAAFDGFGTTGNLN 120

Query: 121 TRKREVAAFLAQTSHETKGGWQTAPDGPYAWGYCYINSTIKDASCSPSTNWPCASGKQYY 180
            RKREVAAFLAQTSHET GGWQTAPDGPYAWGYCYINSTIKDASCSPSTNWPCASGKQYY
Sbjct: 121 IRKREVAAFLAQTSHETTGGWQTAPDGPYAWGYCYINSTIKDASCSPSTNWPCASGKQYY 180

Query: 181 GRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDVM 240
           GRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDVM
Sbjct: 181 GRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDVM 240

Query: 241 AGKWVPSNEDISAKRLPGYGLTINLINASECVDGTDDRAADRIGFYKRYCDILNIQYGDN 300
            GKWVPSNEDISAKRLPGYGLTINLIN SECV+GTDDRAADRIGFYKRYCDILNIQYGDN
Sbjct: 241 TGKWVPSNEDISAKRLPGYGLTINLINVSECVNGTDDRAADRIGFYKRYCDILNIQYGDN 300

Query: 301 LDCVGQQPF 309
           LDCVGQQPF
Sbjct: 301 LDCVGQQPF 306

BLAST of Cp4.1LG14g10490 vs. NCBI nr
Match: KAG6577805.1 (Endochitinase A2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 625 bits (1611), Expect = 7.92e-226
Identity = 293/310 (94.52%), Postives = 298/310 (96.13%), Query Frame = 0

Query: 1   MRKIYRSFVALAFVLFTLATSWAQPCGGGGR-EPENAMCPKGLCSAGSGFCRSGFVRRCG 60
           MRKIY SF+ALAFVLFT ATSWAQPCGGGG  EPENAMCPKGLCSAGSGFCRSGFV+RCG
Sbjct: 1   MRKIYPSFLALAFVLFTSATSWAQPCGGGGGGEPENAMCPKGLCSAGSGFCRSGFVQRCG 60

Query: 61  SDLTFEGPIGEIGGVISASDFDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFDGFGTTGNL 120
           SDLTF  PIGEIG VISASDFDEMLKHRNDR+CKSNGFYSYEAFAVAAAAFDGFGTTGNL
Sbjct: 61  SDLTFGSPIGEIGSVISASDFDEMLKHRNDRSCKSNGFYSYEAFAVAAAAFDGFGTTGNL 120

Query: 121 NTRKREVAAFLAQTSHETKGGWQTAPDGPYAWGYCYINSTIKDASCSPSTNWPCASGKQY 180
           N RKREVAAFLAQTSHET GGW TAPDGPYAWGYCYI+STIKDASCSPSTNWPCASGKQY
Sbjct: 121 NIRKREVAAFLAQTSHETTGGWPTAPDGPYAWGYCYIDSTIKDASCSPSTNWPCASGKQY 180

Query: 181 YGRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDV 240
           YGRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDV
Sbjct: 181 YGRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDV 240

Query: 241 MAGKWVPSNEDISAKRLPGYGLTINLINASECVDGTDDRAADRIGFYKRYCDILNIQYGD 300
           M GKWVPSNEDISAKRLPGYGLTINLIN SECV+GTDDRAADRIGFYKRYCDILNIQYGD
Sbjct: 241 MTGKWVPSNEDISAKRLPGYGLTINLINVSECVNGTDDRAADRIGFYKRYCDILNIQYGD 300

Query: 301 NLDCVGQQPF 309
           NLDCVGQQPF
Sbjct: 301 NLDCVGQQPF 310

BLAST of Cp4.1LG14g10490 vs. NCBI nr
Match: XP_023007623.1 (basic endochitinase C-like [Cucurbita maxima])

HSP 1 Score: 605 bits (1560), Expect = 4.72e-218
Identity = 283/310 (91.29%), Postives = 292/310 (94.19%), Query Frame = 0

Query: 1   MRKIYRSFVALAFVLFTLATSWAQPCGGGGR-EPENAMCPKGLCSAGSGFCRSGFVRRCG 60
           MRKIYRSFVAL FVLF  ATSWAQPCGGGG  EPENAMCPKGLCSAGSGFC SGFVRRCG
Sbjct: 1   MRKIYRSFVALTFVLFASATSWAQPCGGGGGGEPENAMCPKGLCSAGSGFCGSGFVRRCG 60

Query: 61  SDLTFEGPIGEIGGVISASDFDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFDGFGTTGNL 120
           SDLTF  PIGEIG VIS+SDFDEML+HRNDRNCKSNGFYSYEAFAVAA+AF GFGTTGNL
Sbjct: 61  SDLTFGSPIGEIGSVISSSDFDEMLQHRNDRNCKSNGFYSYEAFAVAASAFVGFGTTGNL 120

Query: 121 NTRKREVAAFLAQTSHETKGGWQTAPDGPYAWGYCYINSTIKDASCSPSTNWPCASGKQY 180
           N RKREVAAFLAQTSHET GGW +APDGPYAWGYCYI+STIKDASC+PS NWPCASGKQY
Sbjct: 121 NIRKREVAAFLAQTSHETTGGWASAPDGPYAWGYCYIDSTIKDASCTPSPNWPCASGKQY 180

Query: 181 YGRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDV 240
           YGRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDV
Sbjct: 181 YGRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDV 240

Query: 241 MAGKWVPSNEDISAKRLPGYGLTINLINASECVDGTDDRAADRIGFYKRYCDILNIQYGD 300
           M G WVPS+ED+SAKRLPGYGLTINLIN SECVDGTDDRAA+RIGFYKRYCDILNIQYGD
Sbjct: 241 MTGNWVPSDEDVSAKRLPGYGLTINLINVSECVDGTDDRAANRIGFYKRYCDILNIQYGD 300

Query: 301 NLDCVGQQPF 309
           NLD VGQQPF
Sbjct: 301 NLDYVGQQPF 310

BLAST of Cp4.1LG14g10490 vs. NCBI nr
Match: KAA0052928.1 (basic endochitinase C [Cucumis melo var. makuwa] >TYK11385.1 basic endochitinase C [Cucumis melo var. makuwa])

HSP 1 Score: 518 bits (1334), Expect = 1.35e-183
Identity = 245/311 (78.78%), Postives = 267/311 (85.85%), Query Frame = 0

Query: 1   MRKIYRS-FVALAFVLFTLATSWAQPCGGGGREPENAMCPKGLCSAGSGFCRSGFVRRCG 60
           M KIY S F+AL F+L T ATS AQ CGGGG EP +AMCPKGLC AGSG C SGFVRRCG
Sbjct: 1   MTKIYTSSFIALTFLLLTPATSSAQSCGGGG-EPADAMCPKGLCIAGSGLCGSGFVRRCG 60

Query: 61  SDLTFEGPIGEIGGVISASDFDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFDGFGTTGNL 120
           SDLTF  P G +GGVISAS+F+ MLKHRND  C+ NGFYSYEAF VAAAAFDGFGTTGNL
Sbjct: 61  SDLTFGTPNGGLGGVISASEFEGMLKHRNDGGCEGNGFYSYEAFVVAAAAFDGFGTTGNL 120

Query: 121 NTRKREVAAFLAQTSHETKGGWQTAPDGPYAWGYCYINSTIKDASCSPSTNWPCASGKQY 180
           NTRKREVAAFLAQTS+ET GGW TAPDGPYAWGYCYIN+T K   C PS NWPC+SGK Y
Sbjct: 121 NTRKREVAAFLAQTSYETTGGWATAPDGPYAWGYCYINATQKYELCVPSPNWPCSSGKLY 180

Query: 181 YGRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDV 240
           YGRGPFQITYN+NYG AG+AIGVDL+NNPDLVATNPTISFKTA+WFWMT  GN PSCH+V
Sbjct: 181 YGRGPFQITYNYNYGPAGQAIGVDLLNNPDLVATNPTISFKTAIWFWMTSHGNIPSCHEV 240

Query: 241 MAGKWVPSNEDISAKRLPGYGLTINLINAS-ECVDGTDDRAADRIGFYKRYCDILNIQYG 300
           M GKWVPSNED+SAKRLPGYGLTINLIN + EC  G D RAADR+GFY+RYCD+LNIQYG
Sbjct: 241 MTGKWVPSNEDVSAKRLPGYGLTINLINGNNECGKGADTRAADRLGFYQRYCDVLNIQYG 300

Query: 301 DNLDCVGQQPF 309
            NL+CV Q+PF
Sbjct: 301 GNLNCVDQKPF 310

BLAST of Cp4.1LG14g10490 vs. ExPASy TrEMBL
Match: A0A6J1E705 (basic endochitinase C-like OS=Cucurbita moschata OX=3662 GN=LOC111431189 PE=4 SV=1)

HSP 1 Score: 625 bits (1613), Expect = 1.64e-226
Identity = 294/309 (95.15%), Postives = 296/309 (95.79%), Query Frame = 0

Query: 1   MRKIYRSFVALAFVLFTLATSWAQPCGGGGREPENAMCPKGLCSAGSGFCRSGFVRRCGS 60
           MRKI   FVALAFVLFT ATSWAQPCGGGG EPENAMCPKGLCSAGSGFCRSGFVRRCGS
Sbjct: 1   MRKI---FVALAFVLFTSATSWAQPCGGGGGEPENAMCPKGLCSAGSGFCRSGFVRRCGS 60

Query: 61  DLTFEGPIGEIGGVISASDFDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFDGFGTTGNLN 120
           DLTF  P GEIG VISASDFDEMLKHRNDR+CKSNGFYSYEAFAVAAAAFDGFGTTGNLN
Sbjct: 61  DLTFGSPTGEIGSVISASDFDEMLKHRNDRSCKSNGFYSYEAFAVAAAAFDGFGTTGNLN 120

Query: 121 TRKREVAAFLAQTSHETKGGWQTAPDGPYAWGYCYINSTIKDASCSPSTNWPCASGKQYY 180
            RKREVAAFLAQTSHET GGWQTAPDGPYAWGYCYINSTIKDASCSPSTNWPCASGKQYY
Sbjct: 121 IRKREVAAFLAQTSHETTGGWQTAPDGPYAWGYCYINSTIKDASCSPSTNWPCASGKQYY 180

Query: 181 GRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDVM 240
           GRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDVM
Sbjct: 181 GRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDVM 240

Query: 241 AGKWVPSNEDISAKRLPGYGLTINLINASECVDGTDDRAADRIGFYKRYCDILNIQYGDN 300
            GKWVPSNEDISAKRLPGYGLTINLIN SECV+GTDDRAADRIGFYKRYCDILNIQYGDN
Sbjct: 241 TGKWVPSNEDISAKRLPGYGLTINLINVSECVNGTDDRAADRIGFYKRYCDILNIQYGDN 300

Query: 301 LDCVGQQPF 309
           LDCVGQQPF
Sbjct: 301 LDCVGQQPF 306

BLAST of Cp4.1LG14g10490 vs. ExPASy TrEMBL
Match: A0A6J1L846 (basic endochitinase C-like OS=Cucurbita maxima OX=3661 GN=LOC111500198 PE=4 SV=1)

HSP 1 Score: 605 bits (1560), Expect = 2.28e-218
Identity = 283/310 (91.29%), Postives = 292/310 (94.19%), Query Frame = 0

Query: 1   MRKIYRSFVALAFVLFTLATSWAQPCGGGGR-EPENAMCPKGLCSAGSGFCRSGFVRRCG 60
           MRKIYRSFVAL FVLF  ATSWAQPCGGGG  EPENAMCPKGLCSAGSGFC SGFVRRCG
Sbjct: 1   MRKIYRSFVALTFVLFASATSWAQPCGGGGGGEPENAMCPKGLCSAGSGFCGSGFVRRCG 60

Query: 61  SDLTFEGPIGEIGGVISASDFDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFDGFGTTGNL 120
           SDLTF  PIGEIG VIS+SDFDEML+HRNDRNCKSNGFYSYEAFAVAA+AF GFGTTGNL
Sbjct: 61  SDLTFGSPIGEIGSVISSSDFDEMLQHRNDRNCKSNGFYSYEAFAVAASAFVGFGTTGNL 120

Query: 121 NTRKREVAAFLAQTSHETKGGWQTAPDGPYAWGYCYINSTIKDASCSPSTNWPCASGKQY 180
           N RKREVAAFLAQTSHET GGW +APDGPYAWGYCYI+STIKDASC+PS NWPCASGKQY
Sbjct: 121 NIRKREVAAFLAQTSHETTGGWASAPDGPYAWGYCYIDSTIKDASCTPSPNWPCASGKQY 180

Query: 181 YGRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDV 240
           YGRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDV
Sbjct: 181 YGRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDV 240

Query: 241 MAGKWVPSNEDISAKRLPGYGLTINLINASECVDGTDDRAADRIGFYKRYCDILNIQYGD 300
           M G WVPS+ED+SAKRLPGYGLTINLIN SECVDGTDDRAA+RIGFYKRYCDILNIQYGD
Sbjct: 241 MTGNWVPSDEDVSAKRLPGYGLTINLINVSECVDGTDDRAANRIGFYKRYCDILNIQYGD 300

Query: 301 NLDCVGQQPF 309
           NLD VGQQPF
Sbjct: 301 NLDYVGQQPF 310

BLAST of Cp4.1LG14g10490 vs. ExPASy TrEMBL
Match: A0A5A7UCL1 (Basic endochitinase C OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00370 PE=4 SV=1)

HSP 1 Score: 518 bits (1334), Expect = 6.52e-184
Identity = 245/311 (78.78%), Postives = 267/311 (85.85%), Query Frame = 0

Query: 1   MRKIYRS-FVALAFVLFTLATSWAQPCGGGGREPENAMCPKGLCSAGSGFCRSGFVRRCG 60
           M KIY S F+AL F+L T ATS AQ CGGGG EP +AMCPKGLC AGSG C SGFVRRCG
Sbjct: 1   MTKIYTSSFIALTFLLLTPATSSAQSCGGGG-EPADAMCPKGLCIAGSGLCGSGFVRRCG 60

Query: 61  SDLTFEGPIGEIGGVISASDFDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFDGFGTTGNL 120
           SDLTF  P G +GGVISAS+F+ MLKHRND  C+ NGFYSYEAF VAAAAFDGFGTTGNL
Sbjct: 61  SDLTFGTPNGGLGGVISASEFEGMLKHRNDGGCEGNGFYSYEAFVVAAAAFDGFGTTGNL 120

Query: 121 NTRKREVAAFLAQTSHETKGGWQTAPDGPYAWGYCYINSTIKDASCSPSTNWPCASGKQY 180
           NTRKREVAAFLAQTS+ET GGW TAPDGPYAWGYCYIN+T K   C PS NWPC+SGK Y
Sbjct: 121 NTRKREVAAFLAQTSYETTGGWATAPDGPYAWGYCYINATQKYELCVPSPNWPCSSGKLY 180

Query: 181 YGRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDV 240
           YGRGPFQITYN+NYG AG+AIGVDL+NNPDLVATNPTISFKTA+WFWMT  GN PSCH+V
Sbjct: 181 YGRGPFQITYNYNYGPAGQAIGVDLLNNPDLVATNPTISFKTAIWFWMTSHGNIPSCHEV 240

Query: 241 MAGKWVPSNEDISAKRLPGYGLTINLINAS-ECVDGTDDRAADRIGFYKRYCDILNIQYG 300
           M GKWVPSNED+SAKRLPGYGLTINLIN + EC  G D RAADR+GFY+RYCD+LNIQYG
Sbjct: 241 MTGKWVPSNEDVSAKRLPGYGLTINLINGNNECGKGADTRAADRLGFYQRYCDVLNIQYG 300

Query: 301 DNLDCVGQQPF 309
            NL+CV Q+PF
Sbjct: 301 GNLNCVDQKPF 310

BLAST of Cp4.1LG14g10490 vs. ExPASy TrEMBL
Match: A0A1S3BKQ5 (basic endochitinase C OS=Cucumis melo OX=3656 GN=LOC103490661 PE=4 SV=1)

HSP 1 Score: 518 bits (1334), Expect = 2.09e-183
Identity = 245/311 (78.78%), Postives = 267/311 (85.85%), Query Frame = 0

Query: 1   MRKIYRS-FVALAFVLFTLATSWAQPCGGGGREPENAMCPKGLCSAGSGFCRSGFVRRCG 60
           M KIY S F+AL F+L T ATS AQ CGGGG EP +AMCPKGLC AGSG C SGFVRRCG
Sbjct: 33  MTKIYTSSFIALTFLLLTPATSSAQSCGGGG-EPADAMCPKGLCIAGSGLCGSGFVRRCG 92

Query: 61  SDLTFEGPIGEIGGVISASDFDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFDGFGTTGNL 120
           SDLTF  P G +GGVISAS+F+ MLKHRND  C+ NGFYSYEAF VAAAAFDGFGTTGNL
Sbjct: 93  SDLTFGTPNGGLGGVISASEFEGMLKHRNDGGCEGNGFYSYEAFVVAAAAFDGFGTTGNL 152

Query: 121 NTRKREVAAFLAQTSHETKGGWQTAPDGPYAWGYCYINSTIKDASCSPSTNWPCASGKQY 180
           NTRKREVAAFLAQTS+ET GGW TAPDGPYAWGYCYIN+T K   C PS NWPC+SGK Y
Sbjct: 153 NTRKREVAAFLAQTSYETTGGWATAPDGPYAWGYCYINATQKYELCVPSPNWPCSSGKLY 212

Query: 181 YGRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDV 240
           YGRGPFQITYN+NYG AG+AIGVDL+NNPDLVATNPTISFKTA+WFWMT  GN PSCH+V
Sbjct: 213 YGRGPFQITYNYNYGPAGQAIGVDLLNNPDLVATNPTISFKTAIWFWMTSHGNIPSCHEV 272

Query: 241 MAGKWVPSNEDISAKRLPGYGLTINLINAS-ECVDGTDDRAADRIGFYKRYCDILNIQYG 300
           M GKWVPSNED+SAKRLPGYGLTINLIN + EC  G D RAADR+GFY+RYCD+LNIQYG
Sbjct: 273 MTGKWVPSNEDVSAKRLPGYGLTINLINGNNECGKGADTRAADRLGFYQRYCDVLNIQYG 332

Query: 301 DNLDCVGQQPF 309
            NL+CV Q+PF
Sbjct: 333 GNLNCVDQKPF 342

BLAST of Cp4.1LG14g10490 vs. ExPASy TrEMBL
Match: A0A0A0L646 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G002420 PE=4 SV=1)

HSP 1 Score: 515 bits (1327), Expect = 8.16e-183
Identity = 242/311 (77.81%), Postives = 265/311 (85.21%), Query Frame = 0

Query: 1   MRKIYRSFVALAFVLFTL-ATSWAQPCGGGGREPENAMCPKGLCSAGSGFCRSGFVRRCG 60
           M KIY SF+ L FVL T  ATS AQ CGGG  E  +AMCPKGLC AGSG C SGF+RRCG
Sbjct: 1   MTKIYTSFITLTFVLLTTPATSSAQSCGGG--ESADAMCPKGLCIAGSGLCGSGFLRRCG 60

Query: 61  SDLTFEGPIGEIGGVISASDFDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFDGFGTTGNL 120
           SDLTF  P   +GGV+SAS+FD MLKHRND +C+ NGFYSYEAF VAAA FDGFGTTGNL
Sbjct: 61  SDLTFGNPNEGMGGVVSASEFDGMLKHRNDGSCEGNGFYSYEAFVVAAAEFDGFGTTGNL 120

Query: 121 NTRKREVAAFLAQTSHETKGGWQTAPDGPYAWGYCYINSTIKDASCSPSTNWPCASGKQY 180
           +TRKREVAAFLAQTS+ET GGW TAPDGPYAWGYCYIN+T KD  C PS  WPC+SGK Y
Sbjct: 121 STRKREVAAFLAQTSYETTGGWATAPDGPYAWGYCYINATQKDQFCVPSPTWPCSSGKLY 180

Query: 181 YGRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDV 240
           YGRGPFQI YNFNYGAAG+AIGVDL+NNPDLVATNPTISFKTALWFWMT QG+KPSCHDV
Sbjct: 181 YGRGPFQIAYNFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDV 240

Query: 241 MAGKWVPSNEDISAKRLPGYGLTINLINAS-ECVDGTDDRAADRIGFYKRYCDILNIQYG 300
           M GKWVPSNED+SAKRLPGYGLTIN+IN + EC  G D RAADR+GFY+RYCD+LNIQYG
Sbjct: 241 MTGKWVPSNEDVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYG 300

Query: 301 DNLDCVGQQPF 309
           DN +CV Q+PF
Sbjct: 301 DNFNCVNQKPF 309

BLAST of Cp4.1LG14g10490 vs. TAIR 10
Match: AT3G12500.1 (basic chitinase )

HSP 1 Score: 361.7 bits (927), Expect = 5.9e-100
Identity = 172/312 (55.13%), Postives = 213/312 (68.27%), Query Frame = 0

Query: 8   FVALAFVLFTLATSWAQPCGGGGREPENAMCPKGLCSAGSGFC--------RSGFVRRCG 67
           F+ L F L  L+ S A+ C   GR+   A+CP GLC +  G+C        + G   +C 
Sbjct: 19  FLFLIFSLL-LSLSSAEQC---GRQAGGALCPNGLCCSEFGWCGNTEPYCKQPGCQSQCT 78

Query: 68  SDLTFEGPIGEIGGVISASDFDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFDGFGTTGNL 127
              T  GP G++ G+IS+S FD+MLKHRND  C + GFY+Y AF  AA +F GFGTTG+ 
Sbjct: 79  PGGTPPGPTGDLSGIISSSQFDDMLKHRNDAACPARGFYTYNAFITAAKSFPGFGTTGDT 138

Query: 128 NTRKREVAAFLAQTSHETKGGWQTAPDGPYAWGYCYINSTIKDAS-CSPSTNWPCASGKQ 187
            TRK+EVAAF  QTSHET GGW TAPDGPY+WGYC+       +  C PS  WPCASGK+
Sbjct: 139 ATRKKEVAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPASDYCEPSATWPCASGKR 198

Query: 188 YYGRGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHD 247
           YYGRGP Q+++N+NYG  GRAIGVDL+NNPDLVA +  I+FK A+WFWMT Q  KPSCH 
Sbjct: 199 YYGRGPMQLSWNYNYGLCGRAIGVDLLNNPDLVANDAVIAFKAAIWFWMTAQPPKPSCHA 258

Query: 248 VMAGKWVPSNEDISAKRLPGYGLTINLINAS-ECVDGTDDRAADRIGFYKRYCDILNIQY 307
           V+AG+W PS+ D +A RLPGYG+  N+IN   EC  G D R ADRIGFY+RYC+I  +  
Sbjct: 259 VIAGQWQPSDADRAAGRLPGYGVITNIINGGLECGRGQDGRVADRIGFYQRYCNIFGVNP 318

Query: 308 GDNLDCVGQQPF 310
           G NLDC  Q+ F
Sbjct: 319 GGNLDCYNQRSF 326

BLAST of Cp4.1LG14g10490 vs. TAIR 10
Match: AT4G01700.1 (Chitinase family protein )

HSP 1 Score: 271.2 bits (692), Expect = 1.1e-72
Identity = 120/233 (51.50%), Postives = 157/233 (67.38%), Query Frame = 0

Query: 80  FDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFDGFGTTGNLNTRKREVAAFLAQTSHETKG 139
           +D++  H+++  C + GFY YEAF  A  +F  FG+ GN  TR+REVAAFLAQ SHET G
Sbjct: 47  YDQIFIHKDNNACPAKGFYPYEAFVEATRSFPKFGSVGNFWTRRREVAAFLAQISHETTG 106

Query: 140 GWQTAPDGPYAWGYCYINSTIKDASCSPSTN--WPCASGKQYYGRGPFQITYNFNYGAAG 199
           GW TAPDGPYAWG C+       ++   ++N  WPC SGK Y GRGP Q+++N+NYG AG
Sbjct: 107 GWATAPDGPYAWGLCFKEEVSPQSNYCDASNKDWPCVSGKSYKGRGPIQLSWNYNYGQAG 166

Query: 200 RAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDVMAGKWVPSNEDISAKRLP 259
           RA+G D + NP+LVA N  ++FKTALWFWMT Q  KPSCH+VM  ++ P+  D +A R  
Sbjct: 167 RALGFDGLQNPELVANNSVLAFKTALWFWMTEQTPKPSCHNVMVNRYRPTKADRAANRTV 226

Query: 260 GYGLTINLINAS-ECVDGTDDRAADRIGFYKRYCDILNIQYGDNLDCVGQQPF 310
           GYGL  N+IN   EC    D R  DR+G+++RY  +  +  G NLDC  Q+PF
Sbjct: 227 GYGLVTNIINGGLECGIPGDGRVTDRVGYFQRYAQLFKVTTGPNLDCENQRPF 279

BLAST of Cp4.1LG14g10490 vs. TAIR 10
Match: AT1G02360.1 (Chitinase family protein )

HSP 1 Score: 263.8 bits (673), Expect = 1.7e-70
Identity = 119/233 (51.07%), Postives = 158/233 (67.81%), Query Frame = 0

Query: 80  FDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFDGFGTTGNLNTRKREVAAFLAQTSHETKG 139
           ++++  H+++  C +NGFY+YE+F  A   F  FG+ G+  T++ EVAAFLAQ SHET G
Sbjct: 39  YNKIFIHKDNTACPANGFYTYESFVQATRRFPRFGSVGSPVTQRLEVAAFLAQISHETTG 98

Query: 140 GWQTAPDGPYAWGYCYINSTIKDAS-C-SPSTNWPCASGKQYYGRGPFQITYNFNYGAAG 199
           GW TAPDGPYAWG C+       ++ C S  T WPC   K Y GRGP Q+++N+NYG AG
Sbjct: 99  GWATAPDGPYAWGLCFKEEVSPQSTYCDSSDTQWPCFPNKTYQGRGPIQLSWNYNYGPAG 158

Query: 200 RAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDVMAGKWVPSNEDISAKRLP 259
           RA+G D + NP+ V+ N  I+F+TALWFWMTPQ  KPSCHDVM GK+ P+  D++A R  
Sbjct: 159 RALGFDGLRNPETVSNNSVIAFQTALWFWMTPQSPKPSCHDVMIGKYRPTAADLAANRTG 218

Query: 260 GYGLTINLINAS-ECVDGTDDRAADRIGFYKRYCDILNIQYGDNLDCVGQQPF 310
           G+GLT N+IN   EC    D R  DRIGF++RY  +  +  G NLDC  Q+P+
Sbjct: 219 GFGLTTNIINGGLECGIPGDGRVNDRIGFFQRYTGLFKVATGPNLDCENQRPY 271

BLAST of Cp4.1LG14g10490 vs. TAIR 10
Match: AT1G05850.1 (Chitinase family protein )

HSP 1 Score: 180.6 bits (457), Expect = 1.9e-45
Identity = 92/243 (37.86%), Postives = 135/243 (55.56%), Query Frame = 0

Query: 80  FDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFD--GFGTTGNLNTRKREVAAFLAQTSHET 139
           F+++   RN     + GF+ Y++F  AAA F+  GFGTTG     ++E+AAFL   + +T
Sbjct: 69  FEQLFSKRNTPIAHAVGFWDYQSFITAAALFEPLGFGTTGGKLMGQKEMAAFLGHVASKT 128

Query: 140 KGGWQTAPDGPYAWGYCYINSTIKDASCSPSTNW----PCASGKQYYGRGPFQITYNFNY 199
             G+  A  GP AWG CY N  +         +W    PC+ G +YYGRG   I +NFNY
Sbjct: 129 SCGYGVATGGPLAWGLCY-NREMSPMQSYCDESWKFKYPCSPGAEYYGRGALPIYWNFNY 188

Query: 200 GAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTP-QGNKPSCHDVMAGKWVPSNEDIS 259
           GAAG A+  DL+N+P+ +  N T++F+ A+W WMTP +  +PS HD+  G W P+  D  
Sbjct: 189 GAAGEALKADLLNHPEYIEQNATLAFQAAIWRWMTPIKRAQPSAHDIFVGNWKPTKNDTL 248

Query: 260 AKRLPGYGLTINLINAS-ECVDGTDDRAADRIGFYKRYCDILNIQY-----GDNLDCVGQ 310
           +KR P +G T+N++     C  G+ D   + I  Y  + D++ I        D L C  Q
Sbjct: 249 SKRGPTFGSTMNVLYGEYTCGQGSIDPMNNIISHYLYFLDLMGIGREDAGPNDELSCAEQ 308

BLAST of Cp4.1LG14g10490 vs. TAIR 10
Match: AT3G16920.1 (chitinase-like protein 2 )

HSP 1 Score: 176.0 bits (445), Expect = 4.6e-44
Identity = 91/243 (37.45%), Postives = 131/243 (53.91%), Query Frame = 0

Query: 80  FDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFD--GFGTTGNLNTRKREVAAFLAQTSHET 139
           F+ +   RN     + GF+ Y +F  AAA +   GFGT G      +EVAAFL     +T
Sbjct: 92  FENLFSKRNSPVAHAVGFWDYRSFITAAAEYQPLGFGTAGEKLQGMKEVAAFLGHVGSKT 151

Query: 140 KGGWQTAPDGPYAWGYCYINSTIKD-ASCSP--STNWPCASGKQYYGRGPFQITYNFNYG 199
             G+  A  GP AWG CY      D   C       +PC  G  Y+GRG   + +N+NYG
Sbjct: 152 SCGYGVATGGPLAWGLCYNKEMSPDQLYCDDYYKLTYPCTPGVSYHGRGALPVYWNYNYG 211

Query: 200 AAGRAIGVDLINNPDLVATNPTISFKTALWFWMT-PQGNKPSCHDVMAGKWVPSNEDISA 259
             G A+ VDL+++P+ +  N T++F+ A+W WMT P+ + PS HDV  GKW P+  D +A
Sbjct: 212 QTGEALKVDLLSHPEYLENNATLAFQAAIWRWMTPPKKHLPSAHDVFVGKWKPTKNDTAA 271

Query: 260 KRLPGYGLTINLINASE-CVDGTD-DRAADRIGFYKRYCDILNI---QYG--DNLDCVGQ 310
           KR PG+G TIN++   + C  G D D   + +  Y  Y D++ +   + G  + L C  Q
Sbjct: 272 KRTPGFGATINVLYGDQICNSGFDNDEMNNIVSHYLYYLDLIGVGREEAGPHEKLSCADQ 331

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P516134.0e-10158.50Basic endochitinase OS=Vitis vinifera OX=29760 GN=CHIT1B PE=2 SV=1[more]
P053158.9e-10156.13Endochitinase OS=Solanum tuberosum OX=4113 PE=2 SV=1[more]
Q090233.4e-10054.98Endochitinase CH25 OS=Brassica napus OX=3708 PE=2 SV=1[more]
P240919.8e-10056.23Endochitinase B OS=Nicotiana tabacum OX=4097 GN=CHN50 PE=1 SV=1[more]
P212261.7e-9956.07Endochitinase A2 OS=Pisum sativum OX=3888 GN=CHI2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_023553171.16.13e-241100.00basic endochitinase C-like [Cucurbita pepo subsp. pepo][more]
XP_022923516.13.38e-22695.15basic endochitinase C-like [Cucurbita moschata][more]
KAG6577805.17.92e-22694.52Endochitinase A2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023007623.14.72e-21891.29basic endochitinase C-like [Cucurbita maxima][more]
KAA0052928.11.35e-18378.78basic endochitinase C [Cucumis melo var. makuwa] >TYK11385.1 basic endochitinase... [more]
Match NameE-valueIdentityDescription
A0A6J1E7051.64e-22695.15basic endochitinase C-like OS=Cucurbita moschata OX=3662 GN=LOC111431189 PE=4 SV... [more]
A0A6J1L8462.28e-21891.29basic endochitinase C-like OS=Cucurbita maxima OX=3661 GN=LOC111500198 PE=4 SV=1[more]
A0A5A7UCL16.52e-18478.78Basic endochitinase C OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13... [more]
A0A1S3BKQ52.09e-18378.78basic endochitinase C OS=Cucumis melo OX=3656 GN=LOC103490661 PE=4 SV=1[more]
A0A0A0L6468.16e-18377.81Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G002420 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G12500.15.9e-10055.13basic chitinase [more]
AT4G01700.11.1e-7251.50Chitinase family protein [more]
AT1G02360.11.7e-7051.07Chitinase family protein [more]
AT1G05850.11.9e-4537.86Chitinase family protein [more]
AT3G16920.14.6e-4437.45chitinase-like protein 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000726Glycoside hydrolase, family 19, catalyticPFAMPF00182Glyco_hydro_19coord: 74..303
e-value: 1.2E-113
score: 378.7
IPR000726Glycoside hydrolase, family 19, catalyticPROSITEPS00774CHITINASE_19_2coord: 217..227
NoneNo IPR availableGENE3D1.10.530.10coord: 74..303
e-value: 2.4E-109
score: 366.3
NoneNo IPR availableGENE3D3.30.20.10Endochitinase, domain 2coord: 157..213
e-value: 2.4E-109
score: 366.3
NoneNo IPR availablePANTHERPTHR22595:SF171CHITINASE (CLASS IB) / HEVEINcoord: 10..309
NoneNo IPR availablePANTHERPTHR22595CHITINASE-RELATEDcoord: 10..309
NoneNo IPR availableCDDcd00325chitinase_GH19coord: 75..303
e-value: 5.67147E-95
score: 278.545
IPR016283Glycoside hydrolase, family 19PIRSFPIRSF001060Endochitinasecoord: 1..309
e-value: 4.3E-106
score: 352.6
IPR023346Lysozyme-like domain superfamilySUPERFAMILY53955Lysozyme-likecoord: 71..309

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG14g10490.1Cp4.1LG14g10490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0016998 cell wall macromolecule catabolic process
biological_process GO:0006032 chitin catabolic process
biological_process GO:0050832 defense response to fungus
molecular_function GO:0004568 chitinase activity
molecular_function GO:0008061 chitin binding