Cp4.1LG14g08880 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG14g08880
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	beta-amyrin 11-oxidase-like
Location	Cp4.1LG14: 7408289 .. 7414140 (-)
RNA-Seq Expression	Cp4.1LG14g08880
Synteny	Cp4.1LG14g08880

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGATCATAAATGGGGTTTTCTTGATTCTTCCTTTGGGCTTTGTTTTAGTTTTTGAGGTTTTGAAGAGATTGAATGGTTTGTATTATGCTGTGAAGTTGGGGAAGAAATGGGGTGAGCTTCCTCCTGGTGATCTCTCTTGGCCACTTCTTGGTTCTACTTTATCCTTCCTCAAATCTTTCACGGTTGGTCCACCTGAAAGCTTTATTCGTATCTTTACCACCAGGTTTCTCTCTCTTTCTCTTCTCCCGTTCATGTCTATTTTATCATAATTCAATAAAAATCTTCGTTGTTCTACCAAAATGCCTCTAATTTTTTGGGATGGCTGACTTAATGATTGCAATCGCTAGTTCAGAATGACTTTCTAAGTGCTTAAAATTTCATGTGCAATAATTGACTTCCATTATAATGATGTAACATAGTTTATATACTTATGATAGGAACTAAACCAATATGAATAGGATCTTCGTTTTGTAGTTAGATACCAATTATGTTTTGGATATATATATATATATATTTGTTAGGAGAGAAACGGTTAAAGCAACATATTTTTTAGAATTCGTATACATAAGTGTGACGGGAGTTAAATCAATCCAAATTGATCTGTATTTTGTAGTTATAAGTGAATGAGGTATTCGAGAGAGTTGACTTTAAATAGTGTTAGATTTTTTTAATTTTTTCCTAGAGAATAGTTGAAGCAACCTATTTCAAAAAAATCAAACGAGCACATTTTTTAATTTTAACCTACGAGAATAACCGTACACATAAGTATGATAGGAGTGAAAGCAATCCTAACAAGTCAGTATTTTGTAGCTATGAGTGAATGAGGTGTTCAAGAGAGTTGACTACAAAGAGTGTGTTAAATTTTTTTAAACTTTTTTATTTGAGAAGAGTTGAAGTAACATATTTTAAAAAAATCAAACGAGCATGAAATTTTTAATTTTTACCTACAAGAATAACCGTACACGCAAGTGTGATAGGAGTGAAACCAATCTGAACAATATTTTGTAGTTAGAAGTGAATGAGGTGTTTGAGAGAGTTGAACATTTTTTTTTTACCTTAATCTTTATTGAGGTAGGTATGGGAAAGTTGACATGTACAAGACTCACATGTTTGGGAGAGCAAGCATACTCGTCTGCAAACCTGAAATCTGTCGCCAAGTACTAATTGATGAAACCAAATTTGTTCCTAGTTATCCCGCCTGCATGAAAAGGTTATTTGGTAGAAAGTCACTGATCCGAGTCTCCAAGGCGGAGCACCGGAAGCTCCGACGGATGACAATGGCGCCGATCAGCAGCCACGCTGCGCTCGAGATCTACATCGAGCATACTGAACACACAGTGATCAGTGGGTTGGAGGAATGGTCAAGTGTGGAGAAGCCATTGAAGCTGTTGACAGAGATAAAGGAGCTTACCTTCAAAATTATTTGGAACATTTTTATGGGTTCAACTTCCATAGACTATACCACAAAAGAAATGGAGGCTTTGTATGATGATGTTGCACTTGGCTTATTCTGTTTCCCCATCAACTTTCCTGGCTTCAACTTCCATAAATCTCTCAAGGTAACCACTTTCTTTTCCTTCTAAATATGTATATTAGACGAGAATGTCACGGTTCAATCAGCTAAAATATTATACTATCTAGTACATGTTATAGATTCAAATCTGAATAGTATATGTCTTACCCCGAAAAGTATCTTCTAACCTATGATATTTAGGTGATCCGTTTATGAATTGCAACTTTTGAAAAAATTTAATTATTTTTCAAAGCAAATCTTTTATCATGCAAATCTTTTATCGTCAGTCTCAGGGTTTTAAAGCGCGTATGCTAGGGAGAGGTTTCCACACCCTTATAAGAAATGTTTCGTTCCCCTCTCCAACTGACGTGGAATCTCATAATCCACTCCCTTGGAGACCAGTGTCCTCGTTGGGCTTTGATACCATTTGTAACCACTCAAGCTCACCACTAGATATTGTCCGCTTTAGCCCGTTACATATCATCGTCAACCTCACGATTTTAAAGTCCGTCTGCTAGACAGAGGTTTTCACACGCTTATAAGGAATGTTTTGTTCCCCTCTTCAACCGATGTGGGATCTCACATATAAATTTTTCATCAATCCCATATATGATCGATTAACGAAAATAAATGTATGAATACAGGCTCGAAAAAGATTGTATGAAATACTTCTATCTATTGTGAACGAGAAGAGGTTAATGAAGAAAAGCAAAGGGGAAAGTTGGGAAGCAAAAGATATGATGGATTTGATGACAGAAGTGGAAGATGAAGATGGTGAAGGGATGGACAATGAGACCATTACAGATTTGATTTTTGGTAAGTTATTTGCAGGCCATGAGACTTCAGCTTTCACTACAATGTGGGCAATTATATTTCTTACAGATCATCCACATATATTTCAAAGGGCCAAGGTTAGAGTTCTTTAATTTTCTAACTTGTTAACTGCATTCATTTGTCTAAATCTTATGATCTCTTTATTTTTCTTAGTTATTGCGTATTAATTTTGTCTCGAATACGTTTTTGGTCTCTTTAATTTTGTGATACAAGAATATTTGGCTCGTAGGCTAGAGTTGTGTAGTCTAAGGTTAGGAAATATACTTGTTTATCCAAAGTTATGGGGTTCACGACGACATGAATGTGATGTTCGAGTAAAAGAACAATATATGAATGAACCGAGACCATATCTAAATGATAAAAATTTCGTTAAACATGTTTGACCTAGAATGTAATAGCTCAACCTCATCGCTAGCAGATATTATCCTCTTTAGGCTTTTCCTTTCGGGATTCCCCTCAAGGTTTTTAAAACGCGTCTGTTAAGGAGAGGTTTTTCACACTCTTATAAAGTGTGTTTCGTTCTACTCCCCAAACAATGTGGGATCTCACAATCCATCCCCGTTCATAGCCTAGCCTCCTTCTTGGCGGCACTCATTCCCTTTCTCAATCNGGGCCCCCCCCCCCCTCCAATCCACCCCTTTTGGGGCCAGCGTCCTTGTTGGCATACCGCCTCGTGTCCACCCTCTTCGGGGCTCAACCTCCTCGCTTTTCCATCACCCGGTGTCTGGTTCTAATACCATTTGTAACAGCCAAAGCTCATTGCTACTAGATATTGTCCTCTTTGTGGTTTCCCTTTTGGGCTTCCCCTCGAGGTTTTTAAAACGCGTCTATTAGGAAAAGGTTTCCACACCCTTATAAATAATGTTTAGTTCTCCTCCCGAACCGATGTGAGATCTCACAATATCATGGCAACAAAGTGGTTGGAGGTTGCCTCGGCCCATTGGTTAGGGTTGCTAGAGCAGTTCTCAGGCCTGTCCATACCAAGTAGGGAGTTTGACCATTAGAAATGGTATCAGAGACAGACACCAGACGATGTACCAACGAGGAGATTGAGCCCTGAAGGGGGGTGGACACAAGGCGGTGTGCCAGCAAGGATGTTGGATTCTGAAGGGGGTGGATTGAGTTGTCTCACATCGATTGGAGAAGGGAACGAGTGCCAGAGAAGACGTTGAGCCCCAAAGGGAGTGGACTGTGAGATCCCACATATGTTGAGGAGGAAATGAAATATTTTTGTATAAGGGTGTAAAAACCTCTTCTTAGCAGAGCAGACGCTTTTTAGGCAAAGCCAAAAGAGGACAATATTTGCTAGCGGTGCACTTGTACTGTTATATGAAACAAACAAGTTACAAGACTTGTGTAGAAACTATTATACTCTACTGTGTTTTAGATTTTTTAGATGAAAATCTTACCCGGTTATACACGCTCTTTATTAAGACCAATCATTAAAAATTACATGACAGAACACTTACACTATTTATTGAGACCAATCAATAAGAGATTACAACACTCTGTATAGAAACTATTATACTCAGTGTTTTGAATTTTTTGGATGAAAATCTTAGGGAGAGGTATAGGGGAGACTAGACAATTTATTCCTAAAATATTTAAATATTTATTCAAAAAAATATCTAAACCATTTTCTTAAAATAATTAGATATTTTTATTCCTAAAATACCAATATTTTTCTTAAAATATATACACCTTCCTACAACTATGAGACTTGACTTACTAGGCGAGTGATATATTTTAACACTAATCTCTTGAAAATTTTACTACTAATATGTGCGTGAGACAAAGCTTGCTAAAAAGTTTCAAGACAATTAGGAAATGTGGCTATGTCGTAGGAAATGCAAATAAATGTCCGGTCATCAATTAGGCCTCATTTCTTACAATAAACTTATCTAATTGGTAATAAATAAGAATTTTATTTTCTAATTTTGTTTCTAACAATTAATTAAGCTACATGTTTTAGAATAAGAAACAAATAAGAGGCTAGAATTCGTTCATGCATATGGATTTGTGTTCATATAAATCTTAATCCCTTCGAAAAGCCTAGTGAAATTTAAGATATTGCTCTTTCTAATATTATTTTGGATGATTTTATCATTTTGATGGTAATTTTTAAGATTAATTTGTTTTAGGAAGAACAAGAAGATATCATTAGGCGAAGACCTTCAACACAAAAGGGTATAAATCTCGTCGAGTTTAAACAAATGAAATTTCTTTCTCAGGTATATATTTTTATCTTCAATGTTGGCTAAGATGTTTAAATAAACTACATATCTAAAACATTGTTAATAACGAAATTTTGTATAATTGTAATATCTAGGTGATAGATGAGACGCTTCGTCTTAGTAGTATTGCATTTGCTACTTTTCGTGAGGCAACAACTGATATTAATATCAATGGTTAGTCATCAATGGAGAAAGCTCGGGTTATACGATATCCTTATTTTGATTGATCTCAATCCCGTTGTGCTAAAGAAGGTGAAATTTTATAATAAGCTTGGATTTTCATTTTTTAGGTAAACTTATACCAAAAGGATGGAAAGTTATACTTTGGCATCGAGGGCTGTACATGGATGAAGAGCTACATCCTTCCCCACAAGACTTCAATCCATCGAGATGGGATGTATGCAATCATTTTCCATATTTGCTATCCTTTATTATTGATTTTGTTCTACTTAAAATCATATTCTACATGCAATAAATACATGAAATATAAATGGACTAAACAAAAAAGGGGAAGAATATCATTTAGTCTAAGTTTTGGTTCTTCAATTTCAAAATGTTACTTCTAATTTTGTAGTTTAGTTTTCATTTGGTAGGTTAGAAATTACGAATTTTAAGTATAAGTTTTCATGACTTTGTTCTTAATTTCTCCGAGAGGTCTCATATCAGTGGAGATAATGTTCCTTACTCAAAATATATTTTTTTCTCGCATGTTGTTATATCAGTTGAGTGATTGTTTATAATCATTAATGTTATTTCTTATCATTTGATTATTTATGTTGAACAGGATTTTATAGGCAACCCCGGTGCTTTTACTCCGTTTGGATTAGGAGTCAGGTTGTGCCCTGGACGTGATCTTGCCAAGCTCGAGATCTCAATTTTCCTTCACCATTTTCTCCTCAATTACAAGTGAGTTTTCTCCAACTTTTCTTCTATAAACTTCCAACACAATTAAATATAAGCTTTTAAAAGATGTAGTTGGTGACATTTTTATGTTCCTGGTTCCATTTTTCATATAGAAAAATGTTCTGAAAAAATGAATAACAAGAACTATGAATAGTAACTATATGATTCTCAAGTTCATGATTCAAGAAATAAACATTATCAAACATACCCTAATTTTTCACAATGTATTGTTAATATATACATTTTTGGTGAACGTAGGATCGAGCGATATAATCCACAATGCCAATTGACTTATCTACCACTTCCTCATCCCAAAGACAAATGCCTTGCAAGAGTGCTGAAAGCTGCAGGACAATGTGTATAA

mRNA sequence

ATGGAGATCATAAATGGGGTTTTCTTGATTCTTCCTTTGGGCTTTGTTTTAGTTTTTGAGGTTTTGAAGAGATTGAATGGTTTGTATTATGCTGTGAAGTTGGGGAAGAAATGGGGTGAGCTTCCTCCTGGTGATCTCTCTTGGCCACTTCTTGGTTCTACTTTATCCTTCCTCAAATCTTTCACGGTTGGTCCACCTGAAAGCTTTATTCGTATCTTTACCACCAGGTATGGGAAAGTTGACATGTACAAGACTCACATGTTTGGGAGAGCAAGCATACTCGTCTGCAAACCTGAAATCTGTCGCCAAGTACTAATTGATGAAACCAAATTTGTTCCTAGTTATCCCGCCTGCATGAAAAGGTTATTTGGTAGAAAGTCACTGATCCGAGTCTCCAAGGCGGAGCACCGGAAGCTCCGACGGATGACAATGGCGCCGATCAGCAGCCACGCTGCGCTCGAGATCTACATCGAGCATACTGAACACACAGTGATCAGTGGGTTGGAGGAATGGTCAAGTGTGGAGAAGCCATTGAAGCTGTTGACAGAGATAAAGGAGCTTACCTTCAAAATTATTTGGAACATTTTTATGGGTTCAACTTCCATAGACTATACCACAAAAGAAATGGAGGCTTTGTATGATGATGTTGCACTTGGCTTATTCTGTTTCCCCATCAACTTTCCTGGCTTCAACTTCCATAAATCTCTCAAGGCTCGAAAAAGATTGTATGAAATACTTCTATCTATTGTGAACGAGAAGAGGTTAATGAAGAAAAGCAAAGGGGAAAGTTGGGAAGCAAAAGATATGATGGATTTGATGACAGAAGTGGAAGATGAAGATGGTGAAGGGATGGACAATGAGACCATTACAGATTTGATTTTTGGTAAGTTATTTGCAGGCCATGAGACTTCAGCTTTCACTACAATGTGGGCAATTATATTTCTTACAGATCATCCACATATATTTCAAAGGGCCAAGGAAGAACAAGAAGATATCATTAGGCGAAGACCTTCAACACAAAAGGGTATAAATCTCGTCGAGTTTAAACAAATGAAATTTCTTTCTCAGGTGATAGATGAGACGCTTCGTCTTAGTAGTATTGCATTTGCTACTTTTCGTGAGGCAACAACTGATATTAATATCAATGGTAAACTTATACCAAAAGGATGGAAAGTTATACTTTGGCATCGAGGGCTGTACATGGATGAAGAGCTACATCCTTCCCCACAAGACTTCAATCCATCGAGATGGGATGATTTTATAGGCAACCCCGGTGCTTTTACTCCGTTTGGATTAGGAGTCAGGTTGTGCCCTGGACGTGATCTTGCCAAGCTCGAGATCTCAATTTTCCTTCACCATTTTCTCCTCAATTACAAGATCGAGCGATATAATCCACAATGCCAATTGACTTATCTACCACTTCCTCATCCCAAAGACAAATGCCTTGCAAGAGTGCTGAAAGCTGCAGGACAATGTGTATAA

Coding sequence (CDS)

ATGGAGATCATAAATGGGGTTTTCTTGATTCTTCCTTTGGGCTTTGTTTTAGTTTTTGAGGTTTTGAAGAGATTGAATGGTTTGTATTATGCTGTGAAGTTGGGGAAGAAATGGGGTGAGCTTCCTCCTGGTGATCTCTCTTGGCCACTTCTTGGTTCTACTTTATCCTTCCTCAAATCTTTCACGGTTGGTCCACCTGAAAGCTTTATTCGTATCTTTACCACCAGGTATGGGAAAGTTGACATGTACAAGACTCACATGTTTGGGAGAGCAAGCATACTCGTCTGCAAACCTGAAATCTGTCGCCAAGTACTAATTGATGAAACCAAATTTGTTCCTAGTTATCCCGCCTGCATGAAAAGGTTATTTGGTAGAAAGTCACTGATCCGAGTCTCCAAGGCGGAGCACCGGAAGCTCCGACGGATGACAATGGCGCCGATCAGCAGCCACGCTGCGCTCGAGATCTACATCGAGCATACTGAACACACAGTGATCAGTGGGTTGGAGGAATGGTCAAGTGTGGAGAAGCCATTGAAGCTGTTGACAGAGATAAAGGAGCTTACCTTCAAAATTATTTGGAACATTTTTATGGGTTCAACTTCCATAGACTATACCACAAAAGAAATGGAGGCTTTGTATGATGATGTTGCACTTGGCTTATTCTGTTTCCCCATCAACTTTCCTGGCTTCAACTTCCATAAATCTCTCAAGGCTCGAAAAAGATTGTATGAAATACTTCTATCTATTGTGAACGAGAAGAGGTTAATGAAGAAAAGCAAAGGGGAAAGTTGGGAAGCAAAAGATATGATGGATTTGATGACAGAAGTGGAAGATGAAGATGGTGAAGGGATGGACAATGAGACCATTACAGATTTGATTTTTGGTAAGTTATTTGCAGGCCATGAGACTTCAGCTTTCACTACAATGTGGGCAATTATATTTCTTACAGATCATCCACATATATTTCAAAGGGCCAAGGAAGAACAAGAAGATATCATTAGGCGAAGACCTTCAACACAAAAGGGTATAAATCTCGTCGAGTTTAAACAAATGAAATTTCTTTCTCAGGTGATAGATGAGACGCTTCGTCTTAGTAGTATTGCATTTGCTACTTTTCGTGAGGCAACAACTGATATTAATATCAATGGTAAACTTATACCAAAAGGATGGAAAGTTATACTTTGGCATCGAGGGCTGTACATGGATGAAGAGCTACATCCTTCCCCACAAGACTTCAATCCATCGAGATGGGATGATTTTATAGGCAACCCCGGTGCTTTTACTCCGTTTGGATTAGGAGTCAGGTTGTGCCCTGGACGTGATCTTGCCAAGCTCGAGATCTCAATTTTCCTTCACCATTTTCTCCTCAATTACAAGATCGAGCGATATAATCCACAATGCCAATTGACTTATCTACCACTTCCTCATCCCAAAGACAAATGCCTTGCAAGAGTGCTGAAAGCTGCAGGACAATGTGTATAA

Protein sequence

MEIINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKSFTVGPPESFIRIFTTRYGKVDMYKTHMFGRASILVCKPEICRQVLIDETKFVPSYPACMKRLFGRKSLIRVSKAEHRKLRRMTMAPISSHAALEIYIEHTEHTVISGLEEWSSVEKPLKLLTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKARKRLYEILLSIVNEKRLMKKSKGESWEAKDMMDLMTEVEDEDGEGMDNETITDLIFGKLFAGHETSAFTTMWAIIFLTDHPHIFQRAKEEQEDIIRRRPSTQKGINLVEFKQMKFLSQVIDETLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDFIGNPGAFTPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPLPHPKDKCLARVLKAAGQCV

Homology

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BLAST of Cp4.1LG14g08880 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 3.4e-147
Identity = 247/476 (51.89%), Postives = 350/476 (73.53%), Query Frame = 0


Query: 15  FVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKSFTVGPPESFIRIFT 74
           ++   + ++ LNG YY VKL +K   LPPGD+ WPL+G  LSF+K F+ G P+SFI    
Sbjct: 17  YIFGSKFVRNLNGWYYDVKLRRKEHPLPPGDMGWPLIGDLLSFIKDFSSGHPDSFINNLV 76

Query: 75  TRYGKVDMYKTHMFGRASILVCKPEICRQVLIDETKFVPSYPACMKRLFGRKSLIRVSKA 134
            +YG+  +YKTH+FG  SI+VC+P++CR+VL D+  F   YP  +K L   + +I VS A
Sbjct: 77  LKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIKELARCRPMIDVSNA 136

Query: 135 EHRKLRRMTMAPISSHAALEIYIEHTEHTVISGLEEWSSVEKPLKLLTEIKELTFKIIWN 194
           EHR  RR+  +PI  H AL +Y+E  E  VI+ LEE SS++ P++LL E+K+++FK I +
Sbjct: 137 EHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVELLKEMKKVSFKAIVH 196

Query: 195 IFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKARKRLYEILLSIVNEKR 254
           +FMGS++ D   K++ + + D+  G+F  PIN PGF FHK+L+ARK+L +I+  +V+E+R
Sbjct: 197 VFMGSSNQD-IIKKIGSSFTDLYNGMFSIPINVPGFTFHKALEARKKLAKIVQPVVDERR 256

Query: 255 LM-KKSKGESWEAKDMMDLMTEVEDEDGEGMDNETITDLIFGKLFAGHETSAFTTMWAII 314
           LM +    E  + KD++D++ EV+DE+G  +++E I+DL+ G LFAGHE++A + MW+I 
Sbjct: 257 LMIENGPQEGSQRKDLIDILLEVKDENGRKLEDEDISDLLIGLLFAGHESTATSLMWSIT 316

Query: 315 FLTDHPHIFQRAKEEQEDIIRRRPSTQKGINLVEFKQMKFLSQVIDETLRLSSIAFATFR 374
           +LT HPHI ++AKEEQE+I R R S+QK ++L E KQM +LSQVIDETLR ++IAFATFR
Sbjct: 317 YLTQHPHILKKAKEEQEEITRTRFSSQKQLSLKEIKQMVYLSQVIDETLRCANIAFATFR 376

Query: 375 EATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDFIGNPGAFTPFGLG 434
           EAT D+NING +IPKGW+V++W R ++MD E +P+P++FNPSRWDD+    G F PFG G
Sbjct: 377 EATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPSRWDDYNAKAGTFLPFGAG 436

Query: 435 VRLCPGRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPLPHPKDKCLARVLKAA 490
            RLCPG DLAKLEISIFLH+FL NY++ER NP+C +T LP+  P D CLA+V+K +
Sbjct: 437 SRLCPGADLAKLEISIFLHYFLRNYRLERINPECHVTSLPVSKPTDNCLAKVIKVS 491

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BLAST of Cp4.1LG14g08880 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 454.1 bits (1167), Expect = 2.0e-126
Identity = 225/480 (46.88%), Postives = 321/480 (66.88%), Query Frame = 0


Query: 8   FLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKSFTVGPPE 67
           F ++ LG  ++  VLKR+N   Y  KLG+K   LPPGDL WP++G+  SFL++F    PE
Sbjct: 11  FPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPE 70

Query: 68  SFIRIFTTRYGKVDMYKTHMFGRASILVCKPEICRQVLIDETKFVPSYPACMKRLFGRKS 127
           SFI+ + TRYG+  +YK HMFG   +LV  PE CR+VL D+  F   +P    +L GRKS
Sbjct: 71  SFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKS 130

Query: 128 LIRVSKAEHRKLRRMTMAPISSHAALEIYIEHTEHTVISGLEEWSSVEKPLKLLTEIKEL 187
            + +S  EH++LRR+T AP++   AL +YI+  E TV + LE+WS + + ++ L+ +++L
Sbjct: 131 FVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRKL 190

Query: 188 TFKIIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKARKRLYEILL 247
           TFK+I  IF+ S S ++    +E  Y ++  G+    IN PGF +H++LKARK+L     
Sbjct: 191 TFKVIMYIFLSSES-EHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQ 250

Query: 248 SIVNEKRLMKKSKGESWEAKDMMDLMTEVEDEDGEGMDNETITDLIFGKLFAGHETSAFT 307
           SIV  +R  +K    S   KDM+D + +V+DE+G  +D+E I DL+   L AGHE+S   
Sbjct: 251 SIVTNRRNQRKQNISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHL 310

Query: 308 TMWAIIFLTDHPHIFQRAKEEQEDIIRRRPSTQKGINLVEFKQMKFLSQVIDETLRLSSI 367
           TMWA I + +HP I Q+AKEEQE I+++R   QK + L E ++M +LSQVIDETLR+ + 
Sbjct: 311 TMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITF 370

Query: 368 AFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDFIGNPGAF 427
           +   FREA +D+ ++G +IPKGWKV+ W R +++D E++P P+ F+PSRW+ +    G F
Sbjct: 371 SLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTF 430

Query: 428 TPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPLPHPKDKCLARVLK 487
            PFGLG  LCPG DLAKLEISIFLHHFLL Y++ER NP C + +LP   PKD CLAR+ +
Sbjct: 431 LPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 486

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BLAST of Cp4.1LG14g08880 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 3.7e-125
Identity = 223/479 (46.56%), Postives = 314/479 (65.55%), Query Frame = 0


Query: 8   FLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKSFTVGPPE 67
           F ++ LG   +  +++++N   Y   LG+    LPPGDL WP +G+ LSFL++F    P+
Sbjct: 12  FPLMVLGCFGLNWLVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPD 71

Query: 68  SFIRIFTTRYGKVDMYKTHMFGRASILVCKPEICRQVLIDETKFVPSYPACMKRLFGRKS 127
           SF R    RYG   +YK HMFG  SI+V   + CR+VL D+  F P +P     L GRKS
Sbjct: 72  SFTRTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKS 131

Query: 128 LIRVSKAEHRKLRRMTMAPISSHAALEIYIEHTEHTVISGLEEWSSVEKPLKLLTEIKEL 187
            + +S  EH++LRR+T AP++ H AL  YI + E  VI+ L++W+ + +  + LT +++L
Sbjct: 132 FVGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKL 191

Query: 188 TFKIIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKARKRLYEILL 247
           TF+II  IF+ S S +     +E  Y  +  G+    +N PGF +H++LKARK L     
Sbjct: 192 TFRIIMYIFLSSES-ENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQ 251

Query: 248 SIVNEKRLMKKSKGESWEAKDMMDLMTEVEDEDGEGMDNETITDLIFGKLFAGHETSAFT 307
           SIV E+R  +K    S   KDM+D +  V+DEDG+ +D+E I D++   L AGHE+S  T
Sbjct: 252 SIVTERRNQRKQNILS-NKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHT 311

Query: 308 TMWAIIFLTDHPHIFQRAKEEQEDIIRRRPSTQKGINLVEFKQMKFLSQVIDETLRLSSI 367
            MWA +FL +HP + QRAK EQE I++ RP  QKG++L E ++M+FLSQV+DETLR+ + 
Sbjct: 312 IMWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITF 371

Query: 368 AFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDD-FIGNPGA 427
           +   FREA TD+ +NG LIPKGWKV+ W R +++D E+ P P+ F+P+RWD+ F+   GA
Sbjct: 372 SLTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGA 431

Query: 428 FTPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPLPHPKDKCLARV 486
           F PFG G  LCPG DLAKLEISIFLHHFLL Y+++R NP+C + YLP   P D CLAR+
Sbjct: 432 FLPFGAGSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARI 487

[Close]

BLAST of Cp4.1LG14g08880 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 1.1e-124
Identity = 220/480 (45.83%), Postives = 318/480 (66.25%), Query Frame = 0


Query: 14  GFVLVFEVLKRLNGLYYAVKLG-KKWGELPPGDLSWPLLGSTLSFLKSFTVGPPESFIRI 73
           G VLV  V++R +       LG ++   LPPG++ WP++GS  +FL++F  G P++FI  
Sbjct: 27  GVVLVDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFKSGNPDAFIAS 86

Query: 74  FTTRYGKVDMYKTHMFGRASILVCKPEICRQVLIDETKFVPSYPACMKRLFGRKSLIRVS 133
           F  R+G+  +Y+T MF   +IL   PE C+QVL+D+  FV  +P     L G KS + +S
Sbjct: 87  FIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVTLIGPKSFVNMS 146

Query: 134 KAEHRKLRRMTMAPISSHAALEIYIEHTEHTVISGLEEWSSVEK-PLKLLTEIKELTFKI 193
             +HR++R++T API+   AL  Y+   + TV++ L  WSS E   ++ LTE++ +TFKI
Sbjct: 147 YDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEFLTELRRMTFKI 206

Query: 194 IWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKARKRLYEILLSIVN 253
           I  IFM S + D T + +E  Y D+  G+    IN PGF ++++L+AR++L  +L  +++
Sbjct: 207 IVQIFM-SGADDATMEALERSYTDLNYGMRAMAINLPGFAYYRALRARRKLVSVLQGVLD 266

Query: 254 EKRLMKKSKGESWEAKDMMDLMTEVEDEDGEGMDNETITDLIFGKLFAGHETSAFTTMWA 313
            +R       +   A DMMD + E EDE G  + ++ I D++   L AGHE+S   TMWA
Sbjct: 267 GRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIVDVLIMYLNAGHESSGHITMWA 326

Query: 314 IIFLTDHPHIFQRAKEEQEDIIRRRPSTQKGINLVEFKQMKFLSQVIDETLRLSSIAFAT 373
            +FL ++P IF RAK EQE+I+R  P+TQ G+ L +FK+M FLSQV+DETLR  +I+F +
Sbjct: 327 TVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQVVDETLRCVNISFVS 386

Query: 374 FREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDFIGNPGAFTPFG 433
           FR+AT DI +NG LIPKGWKV LW+R ++MD++++P P+ FNPSRW+      G F PFG
Sbjct: 387 FRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWEGPPPKAGTFLPFG 446

Query: 434 LGVRLCPGRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPLPHPKDKCLARVLKAAGQ 492
           LG RLCPG DLAKLEIS+FLHHFLL YK++R NP+C++ YLP P P D CLA + K + +
Sbjct: 447 LGARLCPGNDLAKLEISVFLHHFLLGYKLKRANPKCRVRYLPHPRPVDNCLATITKVSDE 505

[Close]

BLAST of Cp4.1LG14g08880 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 3.6e-120
Identity = 212/480 (44.17%), Postives = 313/480 (65.21%), Query Frame = 0


Query: 9   LILPLGFVLVFEVLKRLNGLYYAVKLG-KKWGELPPGDLSWPLLGSTLSFLKSFTVGPPE 68
           L+  +  V+V    +R +G Y    LG  +   LPPG++ WPL+G   +FL++F  G P+
Sbjct: 39  LVGAVALVVVDAAARRAHGWYREAALGAARRARLPPGEMGWPLVGGMWAFLRAFKSGKPD 98

Query: 69  SFIRIFTTRYGKVDMYKTHMFGRASILVCKPEICRQVLIDETKFVPSYPACMKRLFGRKS 128
           +FI  F  R+G+  +Y++ MF   ++LV   E C+QVL+D+  FV  +P     L G +S
Sbjct: 99  AFIASFVRRFGRTGVYRSFMFSSPTVLVTTAEGCKQVLMDDDAFVTGWPKATVALVGPRS 158

Query: 129 LIRVSKAEHRKLRRMTMAPISSHAALEIYIEHTEHTVISGLEEWSSVEKPLKLLTEIKEL 188
            + +   EHR++R++T API+   AL  Y+   + TV S L  W+     ++ LTE++ +
Sbjct: 159 FVAMPYDEHRRIRKLTAAPINGFDALTGYLPFIDRTVTSSLRAWADHGGSVEFLTELRRM 218

Query: 189 TFKIIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKARKRLYEILL 248
           TFKII  IF+G      TT+ +E  Y ++  G+    IN PGF +  +L+AR+RL  +L 
Sbjct: 219 TFKIIVQIFLGGAD-QATTRALERSYTELNYGMRAMAINLPGFAYRGALRARRRLVAVLQ 278

Query: 249 SIVNEKRLMKKSKGESWEAKDMMDLMTEVEDEDGEGMDNETITDLIFGKLFAGHETSAFT 308
            +++E+R   ++KG S    DMMD + E +DE G  +D++ I D++   L AGHE+S   
Sbjct: 279 GVLDERR-AARAKGVSGGGVDMMDRLIEAQDERGRHLDDDEIIDVLVMYLNAGHESSGHI 338

Query: 309 TMWAIIFLTDHPHIFQRAKEEQEDIIRRRPSTQKGINLVEFKQMKFLSQVIDETLRLSSI 368
           TMWA +FL ++P +F RAK EQE I+R  PS+Q+G+ L +F++M++LSQVIDETLRL +I
Sbjct: 339 TMWATVFLQENPDMFARAKAEQEAIMRSIPSSQRGLTLRDFRKMEYLSQVIDETLRLVNI 398

Query: 369 AFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDFIGNPGAF 428
           +F +FR+AT D+ +NG LIPKGWKV LW+R ++MD +++P P  F+PSRW+      G F
Sbjct: 399 SFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPTKFDPSRWEGHSPRAGTF 458

Query: 429 TPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPLPHPKDKCLARVLK 488
             FGLG RLCPG DLAKLEIS+FLHHFLL YK+ R NP+C++ YLP P P D CLA++ +
Sbjct: 459 LAFGLGARLCPGNDLAKLEISVFLHHFLLGYKLARTNPRCRVRYLPHPRPVDNCLAKITR 516

[Close]

BLAST of Cp4.1LG14g08880 vs. NCBI nr
Match: XP_023552974.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1001 bits (2587), Expect = 0.0
Identity = 492/492 (100.00%), Postives = 492/492 (100.00%), Query Frame = 0


Query: 1   MEIINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60
           MEIINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS
Sbjct: 1   MEIINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60

Query: 61  FTVGPPESFIRIFTTRYGKVDMYKTHMFGRASILVCKPEICRQVLIDETKFVPSYPACMK 120
           FTVGPPESFIRIFTTRYGKVDMYKTHMFGRASILVCKPEICRQVLIDETKFVPSYPACMK
Sbjct: 61  FTVGPPESFIRIFTTRYGKVDMYKTHMFGRASILVCKPEICRQVLIDETKFVPSYPACMK 120

Query: 121 RLFGRKSLIRVSKAEHRKLRRMTMAPISSHAALEIYIEHTEHTVISGLEEWSSVEKPLKL 180
           RLFGRKSLIRVSKAEHRKLRRMTMAPISSHAALEIYIEHTEHTVISGLEEWSSVEKPLKL
Sbjct: 121 RLFGRKSLIRVSKAEHRKLRRMTMAPISSHAALEIYIEHTEHTVISGLEEWSSVEKPLKL 180

Query: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKARK 240
           LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKARK
Sbjct: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKARK 240

Query: 241 RLYEILLSIVNEKRLMKKSKGESWEAKDMMDLMTEVEDEDGEGMDNETITDLIFGKLFAG 300
           RLYEILLSIVNEKRLMKKSKGESWEAKDMMDLMTEVEDEDGEGMDNETITDLIFGKLFAG
Sbjct: 241 RLYEILLSIVNEKRLMKKSKGESWEAKDMMDLMTEVEDEDGEGMDNETITDLIFGKLFAG 300

Query: 301 HETSAFTTMWAIIFLTDHPHIFQRAKEEQEDIIRRRPSTQKGINLVEFKQMKFLSQVIDE 360
           HETSAFTTMWAIIFLTDHPHIFQRAKEEQEDIIRRRPSTQKGINLVEFKQMKFLSQVIDE
Sbjct: 301 HETSAFTTMWAIIFLTDHPHIFQRAKEEQEDIIRRRPSTQKGINLVEFKQMKFLSQVIDE 360

Query: 361 TLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDF 420
           TLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDF
Sbjct: 361 TLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDF 420

Query: 421 IGNPGAFTPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPLPHPKDK 480
           IGNPGAFTPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPLPHPKDK
Sbjct: 421 IGNPGAFTPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPLPHPKDK 480

Query: 481 CLARVLKAAGQC 492
           CLARVLKAAGQC
Sbjct: 481 CLARVLKAAGQC 492

[Close]

BLAST of Cp4.1LG14g08880 vs. NCBI nr
Match: XP_023552973.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 907 bits (2345), Expect = 0.0
Identity = 444/489 (90.80%), Postives = 465/489 (95.09%), Query Frame = 0


Query: 1   MEIINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60
           ME INGVFLILPLGFVLVFEVLKRLNGLYYA+KLGKKWGELPPGDLSWPLLGSTLSFLKS
Sbjct: 1   MEFINGVFLILPLGFVLVFEVLKRLNGLYYALKLGKKWGELPPGDLSWPLLGSTLSFLKS 60

Query: 61  FTVGPPESFIRIFTTRYGKVDMYKTHMFGRASILVCKPEICRQVLIDETKFVPSYPACMK 120
           FTVGPPESFIR FTTRYGKVDMYKTH+FGRASILVCKPEICRQVLI+ETKFVP YPA MK
Sbjct: 61  FTVGPPESFIRAFTTRYGKVDMYKTHIFGRASILVCKPEICRQVLINETKFVPGYPASMK 120

Query: 121 RLFGRKSLIRVSKAEHRKLRRMTMAPISSHAALEIYIEHTEHTVISGLEEWSSVEKPLKL 180
            LFGRKSL RVSKAEHRKLRR+T APIS HAALE+YIEH +HTVI+GLEEWSSVEKPLKL
Sbjct: 121 TLFGRKSLHRVSKAEHRKLRRVTTAPISGHAALEMYIEHIKHTVINGLEEWSSVEKPLKL 180

Query: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKARK 240
           LTEIKELTFKIIWNIFMGSTSID+TTKEMEALY+D++LG F  PINFPGF FHKSLKARK
Sbjct: 181 LTEIKELTFKIIWNIFMGSTSIDFTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARK 240

Query: 241 RLYEILLSIVNEKRLMKKSKGESWEAKDMMDLMTEVEDEDGEGMDNETITDLIFGKLFAG 300
           RL+EILLSIVNEKRLMKKSKGESWEA+DMMDLM EVEDEDGEGMDNETI DLIFGKLFAG
Sbjct: 241 RLHEILLSIVNEKRLMKKSKGESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAG 300

Query: 301 HETSAFTTMWAIIFLTDHPHIFQRAKEEQEDIIRRRPSTQKGINLVEFKQMKFLSQVIDE 360
            ETSAFTTMWAIIFLTDHPHIFQ+AKEEQEDI+ RRPSTQKGINL EFKQMKFLSQVIDE
Sbjct: 301 QETSAFTTMWAIIFLTDHPHIFQKAKEEQEDILSRRPSTQKGINLAEFKQMKFLSQVIDE 360

Query: 361 TLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDF 420
           TLRLS I FATFREATTD+NINGKLIPKGWKVILW R LYMDEELHP PQDFNPSRWD+F
Sbjct: 361 TLRLSGITFATFREATTDVNINGKLIPKGWKVILWLRELYMDEELHPFPQDFNPSRWDNF 420

Query: 421 IGNPGAFTPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPLPHPKDK 480
           IGNPGAFTPFGLGVR+CPGRDLAKLEISIFLH+FLLNYKIERYNPQCQLTYLP+P+PKDK
Sbjct: 421 IGNPGAFTPFGLGVRMCPGRDLAKLEISIFLHYFLLNYKIERYNPQCQLTYLPIPYPKDK 480

Query: 481 CLARVLKAA 489
           CLARVLK A
Sbjct: 481 CLARVLKTA 489

[Close]

BLAST of Cp4.1LG14g08880 vs. NCBI nr
Match: XP_023007801.1 (beta-amyrin 11-oxidase-like [Cucurbita maxima])

HSP 1 Score: 907 bits (2343), Expect = 0.0
Identity = 443/489 (90.59%), Postives = 462/489 (94.48%), Query Frame = 0


Query: 1   MEIINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60
           ME INGVFLILPLGFVLVFEVLKRLNGLYYA+KLGKKWGELPPGDLSWPLLGSTLSFLKS
Sbjct: 1   MEFINGVFLILPLGFVLVFEVLKRLNGLYYALKLGKKWGELPPGDLSWPLLGSTLSFLKS 60

Query: 61  FTVGPPESFIRIFTTRYGKVDMYKTHMFGRASILVCKPEICRQVLIDETKFVPSYPACMK 120
           FTVGPPESFIR FTTRYGKVDMYKTH+FGRASI+VCKPEICRQVLIDETKFVP YPA MK
Sbjct: 61  FTVGPPESFIRAFTTRYGKVDMYKTHIFGRASIIVCKPEICRQVLIDETKFVPGYPASMK 120

Query: 121 RLFGRKSLIRVSKAEHRKLRRMTMAPISSHAALEIYIEHTEHTVISGLEEWSSVEKPLKL 180
            LFGRKSL RVSK EHRKLRR+T APIS HAALE+YIEH EHTVISGLEEWSSVEKPLKL
Sbjct: 121 TLFGRKSLHRVSKVEHRKLRRVTTAPISGHAALEMYIEHIEHTVISGLEEWSSVEKPLKL 180

Query: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKARK 240
           LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALY+D++LG F  PINFPGF FHKSLKARK
Sbjct: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARK 240

Query: 241 RLYEILLSIVNEKRLMKKSKGESWEAKDMMDLMTEVEDEDGEGMDNETITDLIFGKLFAG 300
           RL+EILLSIVNEKRLMKKSKGESWEA+DMMDLM EVEDEDGEGMDNETI DLIFGKLFAG
Sbjct: 241 RLHEILLSIVNEKRLMKKSKGESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAG 300

Query: 301 HETSAFTTMWAIIFLTDHPHIFQRAKEEQEDIIRRRPSTQKGINLVEFKQMKFLSQVIDE 360
            ETSAFTTMWAIIFLTDHPH+FQ+AKEEQEDI+ RRPSTQK IN  EFKQMKFLSQVIDE
Sbjct: 301 QETSAFTTMWAIIFLTDHPHLFQKAKEEQEDILSRRPSTQKSINFAEFKQMKFLSQVIDE 360

Query: 361 TLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDF 420
           TLRLS I FATFREATTD+NINGKLIPKGWKVILW R LYMDEELHPSPQDFNPSRWD+F
Sbjct: 361 TLRLSGITFATFREATTDVNINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWDNF 420

Query: 421 IGNPGAFTPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPLPHPKDK 480
           I NPGAFTPFGLGVR+CPGRDLAKLEISIFLH+FLLNYKIERYNPQCQLTYLP+P+PKDK
Sbjct: 421 ISNPGAFTPFGLGVRMCPGRDLAKLEISIFLHYFLLNYKIERYNPQCQLTYLPIPYPKDK 480

Query: 481 CLARVLKAA 489
           CLARVLK A
Sbjct: 481 CLARVLKTA 489

[Close]

BLAST of Cp4.1LG14g08880 vs. NCBI nr
Match: XP_022923281.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])

HSP 1 Score: 904 bits (2336), Expect = 0.0
Identity = 444/489 (90.80%), Postives = 461/489 (94.27%), Query Frame = 0


Query: 1   MEIINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60
           ME INGVFLILPLGFVLVFEVLKRLNGLYYA+KLGKKW ELPPGDLSWPLLGSTLSFLKS
Sbjct: 1   MEFINGVFLILPLGFVLVFEVLKRLNGLYYALKLGKKWVELPPGDLSWPLLGSTLSFLKS 60

Query: 61  FTVGPPESFIRIFTTRYGKVDMYKTHMFGRASILVCKPEICRQVLIDETKFVPSYPACMK 120
           FTVGPPESFIR FTTRYGKVDMYKTH+FGRASILVCKPEICRQVLI+ETKFVP YPA MK
Sbjct: 61  FTVGPPESFIRTFTTRYGKVDMYKTHIFGRASILVCKPEICRQVLINETKFVPGYPASMK 120

Query: 121 RLFGRKSLIRVSKAEHRKLRRMTMAPISSHAALEIYIEHTEHTVISGLEEWSSVEKPLKL 180
            LFGRKSL RVSKAEHRKLRR+T APIS HAALE+YIEH EHTVISGLEEWSSVEKPLKL
Sbjct: 121 TLFGRKSLHRVSKAEHRKLRRVTTAPISGHAALEMYIEHIEHTVISGLEEWSSVEKPLKL 180

Query: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKARK 240
           LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALY+D++LG F  PINFPGF FHKSLKARK
Sbjct: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARK 240

Query: 241 RLYEILLSIVNEKRLMKKSKGESWEAKDMMDLMTEVEDEDGEGMDNETITDLIFGKLFAG 300
           RL+EILLSIVNEKRLM KSKGESWEAKDMMDLM EVEDEDGEGMDNETI DLIFGKLFAG
Sbjct: 241 RLHEILLSIVNEKRLMMKSKGESWEAKDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAG 300

Query: 301 HETSAFTTMWAIIFLTDHPHIFQRAKEEQEDIIRRRPSTQKGINLVEFKQMKFLSQVIDE 360
            ETSAFTTMWAIIFLTDHPHIFQ+AKEEQEDI+ RRPST KGINL EFKQMKFLSQVIDE
Sbjct: 301 QETSAFTTMWAIIFLTDHPHIFQKAKEEQEDILSRRPSTHKGINLAEFKQMKFLSQVIDE 360

Query: 361 TLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDF 420
           TLRLS I FATFREATTD+ INGKLIPKGWKVILW R LYMDEELHPSPQDFNPSRWD+F
Sbjct: 361 TLRLSGITFATFREATTDVKINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWDNF 420

Query: 421 IGNPGAFTPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPLPHPKDK 480
           IGNPGAFTPFG GVR+CPGRDLAKLEISIFLH+FLLNYKIERYNPQCQLTYLP+P+PKDK
Sbjct: 421 IGNPGAFTPFGFGVRMCPGRDLAKLEISIFLHYFLLNYKIERYNPQCQLTYLPIPYPKDK 480

Query: 481 CLARVLKAA 489
           CLARVLK A
Sbjct: 481 CLARVLKTA 489

[Close]

BLAST of Cp4.1LG14g08880 vs. NCBI nr
Match: XP_023007793.1 (beta-amyrin 11-oxidase-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 903 bits (2334), Expect = 0.0
Identity = 442/489 (90.39%), Postives = 461/489 (94.27%), Query Frame = 0


Query: 1   MEIINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60
           ME INGVFLILP+GFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDL WPLLGSTLSFLKS
Sbjct: 1   MEFINGVFLILPVGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLCWPLLGSTLSFLKS 60

Query: 61  FTVGPPESFIRIFTTRYGKVDMYKTHMFGRASILVCKPEICRQVLIDETKFVPSYPACMK 120
           FTVGPPESFIR FTTRYGKVDMYKTH+FGRASILVCKPEICRQVL DET FVP YPA M+
Sbjct: 61  FTVGPPESFIRAFTTRYGKVDMYKTHIFGRASILVCKPEICRQVLSDETIFVPGYPASMQ 120

Query: 121 RLFGRKSLIRVSKAEHRKLRRMTMAPISSHAALEIYIEHTEHTVISGLEEWSSVEKPLKL 180
            LFGR +L R SKAEHRKLRR+T APISSHAALE+Y++H EHTVISGLEEWSSVEKPLKL
Sbjct: 121 TLFGRNTLHRASKAEHRKLRRVTTAPISSHAALEMYVDHIEHTVISGLEEWSSVEKPLKL 180

Query: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKARK 240
           LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALY+D++LG F  PINFPGF FHKSLKARK
Sbjct: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARK 240

Query: 241 RLYEILLSIVNEKRLMKKSKGESWEAKDMMDLMTEVEDEDGEGMDNETITDLIFGKLFAG 300
           RLYEILLSIVNEKRLMKKSKGESWEA+DMMDLM EVEDEDGEGMDNETI DLIFGKLFAG
Sbjct: 241 RLYEILLSIVNEKRLMKKSKGESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAG 300

Query: 301 HETSAFTTMWAIIFLTDHPHIFQRAKEEQEDIIRRRPSTQKGINLVEFKQMKFLSQVIDE 360
            ETSAFTTMWAIIFLTDHPH+FQ+AKEEQEDII RRPSTQKGINL EFKQMKFLSQVIDE
Sbjct: 301 QETSAFTTMWAIIFLTDHPHLFQKAKEEQEDIISRRPSTQKGINLAEFKQMKFLSQVIDE 360

Query: 361 TLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDF 420
           TLRLS I FATFREATTD+NINGKLIPKGWKVILW R LYMDEELHPSPQDFNPSRWD+F
Sbjct: 361 TLRLSGIIFATFREATTDVNINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWDNF 420

Query: 421 IGNPGAFTPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPLPHPKDK 480
           IGNPGAFTPFGLGVR+CP RDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLP+P+PKDK
Sbjct: 421 IGNPGAFTPFGLGVRMCPRRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPIPYPKDK 480

Query: 481 CLARVLKAA 489
           CLARVLK A
Sbjct: 481 CLARVLKTA 489

[Close]

BLAST of Cp4.1LG14g08880 vs. ExPASy TrEMBL
Match: A0A6J1L1P3 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111500300 PE=3 SV=1)

HSP 1 Score: 907 bits (2343), Expect = 0.0
Identity = 443/489 (90.59%), Postives = 462/489 (94.48%), Query Frame = 0


Query: 1   MEIINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60
           ME INGVFLILPLGFVLVFEVLKRLNGLYYA+KLGKKWGELPPGDLSWPLLGSTLSFLKS
Sbjct: 1   MEFINGVFLILPLGFVLVFEVLKRLNGLYYALKLGKKWGELPPGDLSWPLLGSTLSFLKS 60

Query: 61  FTVGPPESFIRIFTTRYGKVDMYKTHMFGRASILVCKPEICRQVLIDETKFVPSYPACMK 120
           FTVGPPESFIR FTTRYGKVDMYKTH+FGRASI+VCKPEICRQVLIDETKFVP YPA MK
Sbjct: 61  FTVGPPESFIRAFTTRYGKVDMYKTHIFGRASIIVCKPEICRQVLIDETKFVPGYPASMK 120

Query: 121 RLFGRKSLIRVSKAEHRKLRRMTMAPISSHAALEIYIEHTEHTVISGLEEWSSVEKPLKL 180
            LFGRKSL RVSK EHRKLRR+T APIS HAALE+YIEH EHTVISGLEEWSSVEKPLKL
Sbjct: 121 TLFGRKSLHRVSKVEHRKLRRVTTAPISGHAALEMYIEHIEHTVISGLEEWSSVEKPLKL 180

Query: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKARK 240
           LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALY+D++LG F  PINFPGF FHKSLKARK
Sbjct: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARK 240

Query: 241 RLYEILLSIVNEKRLMKKSKGESWEAKDMMDLMTEVEDEDGEGMDNETITDLIFGKLFAG 300
           RL+EILLSIVNEKRLMKKSKGESWEA+DMMDLM EVEDEDGEGMDNETI DLIFGKLFAG
Sbjct: 241 RLHEILLSIVNEKRLMKKSKGESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAG 300

Query: 301 HETSAFTTMWAIIFLTDHPHIFQRAKEEQEDIIRRRPSTQKGINLVEFKQMKFLSQVIDE 360
            ETSAFTTMWAIIFLTDHPH+FQ+AKEEQEDI+ RRPSTQK IN  EFKQMKFLSQVIDE
Sbjct: 301 QETSAFTTMWAIIFLTDHPHLFQKAKEEQEDILSRRPSTQKSINFAEFKQMKFLSQVIDE 360

Query: 361 TLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDF 420
           TLRLS I FATFREATTD+NINGKLIPKGWKVILW R LYMDEELHPSPQDFNPSRWD+F
Sbjct: 361 TLRLSGITFATFREATTDVNINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWDNF 420

Query: 421 IGNPGAFTPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPLPHPKDK 480
           I NPGAFTPFGLGVR+CPGRDLAKLEISIFLH+FLLNYKIERYNPQCQLTYLP+P+PKDK
Sbjct: 421 ISNPGAFTPFGLGVRMCPGRDLAKLEISIFLHYFLLNYKIERYNPQCQLTYLPIPYPKDK 480

Query: 481 CLARVLKAA 489
           CLARVLK A
Sbjct: 481 CLARVLKTA 489

[Close]

BLAST of Cp4.1LG14g08880 vs. ExPASy TrEMBL
Match: A0A6J1E6E2 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111431022 PE=3 SV=1)

HSP 1 Score: 904 bits (2336), Expect = 0.0
Identity = 444/489 (90.80%), Postives = 461/489 (94.27%), Query Frame = 0


Query: 1   MEIINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60
           ME INGVFLILPLGFVLVFEVLKRLNGLYYA+KLGKKW ELPPGDLSWPLLGSTLSFLKS
Sbjct: 1   MEFINGVFLILPLGFVLVFEVLKRLNGLYYALKLGKKWVELPPGDLSWPLLGSTLSFLKS 60

Query: 61  FTVGPPESFIRIFTTRYGKVDMYKTHMFGRASILVCKPEICRQVLIDETKFVPSYPACMK 120
           FTVGPPESFIR FTTRYGKVDMYKTH+FGRASILVCKPEICRQVLI+ETKFVP YPA MK
Sbjct: 61  FTVGPPESFIRTFTTRYGKVDMYKTHIFGRASILVCKPEICRQVLINETKFVPGYPASMK 120

Query: 121 RLFGRKSLIRVSKAEHRKLRRMTMAPISSHAALEIYIEHTEHTVISGLEEWSSVEKPLKL 180
            LFGRKSL RVSKAEHRKLRR+T APIS HAALE+YIEH EHTVISGLEEWSSVEKPLKL
Sbjct: 121 TLFGRKSLHRVSKAEHRKLRRVTTAPISGHAALEMYIEHIEHTVISGLEEWSSVEKPLKL 180

Query: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKARK 240
           LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALY+D++LG F  PINFPGF FHKSLKARK
Sbjct: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARK 240

Query: 241 RLYEILLSIVNEKRLMKKSKGESWEAKDMMDLMTEVEDEDGEGMDNETITDLIFGKLFAG 300
           RL+EILLSIVNEKRLM KSKGESWEAKDMMDLM EVEDEDGEGMDNETI DLIFGKLFAG
Sbjct: 241 RLHEILLSIVNEKRLMMKSKGESWEAKDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAG 300

Query: 301 HETSAFTTMWAIIFLTDHPHIFQRAKEEQEDIIRRRPSTQKGINLVEFKQMKFLSQVIDE 360
            ETSAFTTMWAIIFLTDHPHIFQ+AKEEQEDI+ RRPST KGINL EFKQMKFLSQVIDE
Sbjct: 301 QETSAFTTMWAIIFLTDHPHIFQKAKEEQEDILSRRPSTHKGINLAEFKQMKFLSQVIDE 360

Query: 361 TLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDF 420
           TLRLS I FATFREATTD+ INGKLIPKGWKVILW R LYMDEELHPSPQDFNPSRWD+F
Sbjct: 361 TLRLSGITFATFREATTDVKINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWDNF 420

Query: 421 IGNPGAFTPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPLPHPKDK 480
           IGNPGAFTPFG GVR+CPGRDLAKLEISIFLH+FLLNYKIERYNPQCQLTYLP+P+PKDK
Sbjct: 421 IGNPGAFTPFGFGVRMCPGRDLAKLEISIFLHYFLLNYKIERYNPQCQLTYLPIPYPKDK 480

Query: 481 CLARVLKAA 489
           CLARVLK A
Sbjct: 481 CLARVLKTA 489

[Close]

BLAST of Cp4.1LG14g08880 vs. ExPASy TrEMBL
Match: A0A6J1L8M7 (beta-amyrin 11-oxidase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111500295 PE=3 SV=1)

HSP 1 Score: 903 bits (2334), Expect = 0.0
Identity = 442/489 (90.39%), Postives = 461/489 (94.27%), Query Frame = 0


Query: 1   MEIINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60
           ME INGVFLILP+GFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDL WPLLGSTLSFLKS
Sbjct: 1   MEFINGVFLILPVGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLCWPLLGSTLSFLKS 60

Query: 61  FTVGPPESFIRIFTTRYGKVDMYKTHMFGRASILVCKPEICRQVLIDETKFVPSYPACMK 120
           FTVGPPESFIR FTTRYGKVDMYKTH+FGRASILVCKPEICRQVL DET FVP YPA M+
Sbjct: 61  FTVGPPESFIRAFTTRYGKVDMYKTHIFGRASILVCKPEICRQVLSDETIFVPGYPASMQ 120

Query: 121 RLFGRKSLIRVSKAEHRKLRRMTMAPISSHAALEIYIEHTEHTVISGLEEWSSVEKPLKL 180
            LFGR +L R SKAEHRKLRR+T APISSHAALE+Y++H EHTVISGLEEWSSVEKPLKL
Sbjct: 121 TLFGRNTLHRASKAEHRKLRRVTTAPISSHAALEMYVDHIEHTVISGLEEWSSVEKPLKL 180

Query: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKARK 240
           LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALY+D++LG F  PINFPGF FHKSLKARK
Sbjct: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARK 240

Query: 241 RLYEILLSIVNEKRLMKKSKGESWEAKDMMDLMTEVEDEDGEGMDNETITDLIFGKLFAG 300
           RLYEILLSIVNEKRLMKKSKGESWEA+DMMDLM EVEDEDGEGMDNETI DLIFGKLFAG
Sbjct: 241 RLYEILLSIVNEKRLMKKSKGESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAG 300

Query: 301 HETSAFTTMWAIIFLTDHPHIFQRAKEEQEDIIRRRPSTQKGINLVEFKQMKFLSQVIDE 360
            ETSAFTTMWAIIFLTDHPH+FQ+AKEEQEDII RRPSTQKGINL EFKQMKFLSQVIDE
Sbjct: 301 QETSAFTTMWAIIFLTDHPHLFQKAKEEQEDIISRRPSTQKGINLAEFKQMKFLSQVIDE 360

Query: 361 TLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDF 420
           TLRLS I FATFREATTD+NINGKLIPKGWKVILW R LYMDEELHPSPQDFNPSRWD+F
Sbjct: 361 TLRLSGIIFATFREATTDVNINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWDNF 420

Query: 421 IGNPGAFTPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPLPHPKDK 480
           IGNPGAFTPFGLGVR+CP RDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLP+P+PKDK
Sbjct: 421 IGNPGAFTPFGLGVRMCPRRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPIPYPKDK 480

Query: 481 CLARVLKAA 489
           CLARVLK A
Sbjct: 481 CLARVLKTA 489

[Close]

BLAST of Cp4.1LG14g08880 vs. ExPASy TrEMBL
Match: A0A6J1KZN6 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111500295 PE=3 SV=1)

HSP 1 Score: 897 bits (2318), Expect = 0.0
Identity = 442/494 (89.47%), Postives = 461/494 (93.32%), Query Frame = 0


Query: 1   MEIINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60
           ME INGVFLILP+GFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDL WPLLGSTLSFLKS
Sbjct: 1   MEFINGVFLILPVGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLCWPLLGSTLSFLKS 60

Query: 61  FTVGPPESFIRIFTTR-----YGKVDMYKTHMFGRASILVCKPEICRQVLIDETKFVPSY 120
           FTVGPPESFIR FTTR     YGKVDMYKTH+FGRASILVCKPEICRQVL DET FVP Y
Sbjct: 61  FTVGPPESFIRAFTTRSFSLSYGKVDMYKTHIFGRASILVCKPEICRQVLSDETIFVPGY 120

Query: 121 PACMKRLFGRKSLIRVSKAEHRKLRRMTMAPISSHAALEIYIEHTEHTVISGLEEWSSVE 180
           PA M+ LFGR +L R SKAEHRKLRR+T APISSHAALE+Y++H EHTVISGLEEWSSVE
Sbjct: 121 PASMQTLFGRNTLHRASKAEHRKLRRVTTAPISSHAALEMYVDHIEHTVISGLEEWSSVE 180

Query: 181 KPLKLLTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKS 240
           KPLKLLTEIKELTFKIIWNIFMGSTSIDYTTKEMEALY+D++LG F  PINFPGF FHKS
Sbjct: 181 KPLKLLTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKS 240

Query: 241 LKARKRLYEILLSIVNEKRLMKKSKGESWEAKDMMDLMTEVEDEDGEGMDNETITDLIFG 300
           LKARKRLYEILLSIVNEKRLMKKSKGESWEA+DMMDLM EVEDEDGEGMDNETI DLIFG
Sbjct: 241 LKARKRLYEILLSIVNEKRLMKKSKGESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFG 300

Query: 301 KLFAGHETSAFTTMWAIIFLTDHPHIFQRAKEEQEDIIRRRPSTQKGINLVEFKQMKFLS 360
           KLFAG ETSAFTTMWAIIFLTDHPH+FQ+AKEEQEDII RRPSTQKGINL EFKQMKFLS
Sbjct: 301 KLFAGQETSAFTTMWAIIFLTDHPHLFQKAKEEQEDIISRRPSTQKGINLAEFKQMKFLS 360

Query: 361 QVIDETLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPS 420
           QVIDETLRLS I FATFREATTD+NINGKLIPKGWKVILW R LYMDEELHPSPQDFNPS
Sbjct: 361 QVIDETLRLSGIIFATFREATTDVNINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPS 420

Query: 421 RWDDFIGNPGAFTPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPLP 480
           RWD+FIGNPGAFTPFGLGVR+CP RDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLP+P
Sbjct: 421 RWDNFIGNPGAFTPFGLGVRMCPRRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPIP 480

Query: 481 HPKDKCLARVLKAA 489
           +PKDKCLARVLK A
Sbjct: 481 YPKDKCLARVLKTA 494

[Close]

BLAST of Cp4.1LG14g08880 vs. ExPASy TrEMBL
Match: A0A6J1EBC2 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111431021 PE=3 SV=1)

HSP 1 Score: 892 bits (2305), Expect = 0.0
Identity = 436/489 (89.16%), Postives = 458/489 (93.66%), Query Frame = 0


Query: 1   MEIINGVFLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60
           ME IN VFL+LP+GFVLV+EVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS
Sbjct: 1   MEFINWVFLVLPVGFVLVYEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60

Query: 61  FTVGPPESFIRIFTTRYGKVDMYKTHMFGRASILVCKPEICRQVLIDETKFVPSYPACMK 120
           FTVGPP+++IR FTTRYGKVDMYKTH+FGRASILVC PEICRQVL DET FVP YPA M+
Sbjct: 61  FTVGPPQNYIRTFTTRYGKVDMYKTHIFGRASILVCTPEICRQVLSDETIFVPGYPANMQ 120

Query: 121 RLFGRKSLIRVSKAEHRKLRRMTMAPISSHAALEIYIEHTEHTVISGLEEWSSVEKPLKL 180
            LFGR +L R SKAEHRKLRR+T APISSH ALE+YI+H EHTVISGLEEWSSVEKPLKL
Sbjct: 121 TLFGRNTLHRASKAEHRKLRRVTTAPISSHTALEMYIDHIEHTVISGLEEWSSVEKPLKL 180

Query: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKARK 240
           LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALY+D++LG F  PINFPGF FHKSLKARK
Sbjct: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARK 240

Query: 241 RLYEILLSIVNEKRLMKKSKGESWEAKDMMDLMTEVEDEDGEGMDNETITDLIFGKLFAG 300
           RLYEILLSIVNEKRLMKKSKGESWEAKDMMDLM EVEDEDGEGMDNETI DLIFGKLFAG
Sbjct: 241 RLYEILLSIVNEKRLMKKSKGESWEAKDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAG 300

Query: 301 HETSAFTTMWAIIFLTDHPHIFQRAKEEQEDIIRRRPSTQKGINLVEFKQMKFLSQVIDE 360
            ETSAFTTMWAIIFLTDHPHIFQ+AKEEQEDII RRPSTQKGINL EFKQMKFLSQVIDE
Sbjct: 301 QETSAFTTMWAIIFLTDHPHIFQKAKEEQEDIISRRPSTQKGINLAEFKQMKFLSQVIDE 360

Query: 361 TLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDF 420
           TLRLS + FATFREATTD+NINGKLIPKGWKVILW R LYMDEELH SPQDFNPSRWD+F
Sbjct: 361 TLRLSGVIFATFREATTDVNINGKLIPKGWKVILWLRELYMDEELHSSPQDFNPSRWDNF 420

Query: 421 IGNPGAFTPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPLPHPKDK 480
           IGNPGAFTPFGLGVR+CPGR LAKLEISIFLHHFLLNYKIERYNPQCQLTYLP+P+PKDK
Sbjct: 421 IGNPGAFTPFGLGVRMCPGRHLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPIPYPKDK 480

Query: 481 CLARVLKAA 489
           CLARVLK A
Sbjct: 481 CLARVLKTA 489

[Close]

BLAST of Cp4.1LG14g08880 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 454.1 bits (1167), Expect = 1.4e-127
Identity = 225/480 (46.88%), Postives = 321/480 (66.88%), Query Frame = 0


Query: 8   FLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKSFTVGPPE 67
           F ++ LG  ++  VLKR+N   Y  KLG+K   LPPGDL WP++G+  SFL++F    PE
Sbjct: 11  FPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPE 70

Query: 68  SFIRIFTTRYGKVDMYKTHMFGRASILVCKPEICRQVLIDETKFVPSYPACMKRLFGRKS 127
           SFI+ + TRYG+  +YK HMFG   +LV  PE CR+VL D+  F   +P    +L GRKS
Sbjct: 71  SFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKS 130

Query: 128 LIRVSKAEHRKLRRMTMAPISSHAALEIYIEHTEHTVISGLEEWSSVEKPLKLLTEIKEL 187
            + +S  EH++LRR+T AP++   AL +YI+  E TV + LE+WS + + ++ L+ +++L
Sbjct: 131 FVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRKL 190

Query: 188 TFKIIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKARKRLYEILL 247
           TFK+I  IF+ S S ++    +E  Y ++  G+    IN PGF +H++LKARK+L     
Sbjct: 191 TFKVIMYIFLSSES-EHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQ 250

Query: 248 SIVNEKRLMKKSKGESWEAKDMMDLMTEVEDEDGEGMDNETITDLIFGKLFAGHETSAFT 307
           SIV  +R  +K    S   KDM+D + +V+DE+G  +D+E I DL+   L AGHE+S   
Sbjct: 251 SIVTNRRNQRKQNISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHL 310

Query: 308 TMWAIIFLTDHPHIFQRAKEEQEDIIRRRPSTQKGINLVEFKQMKFLSQVIDETLRLSSI 367
           TMWA I + +HP I Q+AKEEQE I+++R   QK + L E ++M +LSQVIDETLR+ + 
Sbjct: 311 TMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITF 370

Query: 368 AFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDFIGNPGAF 427
           +   FREA +D+ ++G +IPKGWKV+ W R +++D E++P P+ F+PSRW+ +    G F
Sbjct: 371 SLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTF 430

Query: 428 TPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPLPHPKDKCLARVLK 487
            PFGLG  LCPG DLAKLEISIFLHHFLL Y++ER NP C + +LP   PKD CLAR+ +
Sbjct: 431 LPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 486

[Close]

BLAST of Cp4.1LG14g08880 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 454.1 bits (1167), Expect = 1.4e-127
Identity = 225/480 (46.88%), Postives = 321/480 (66.88%), Query Frame = 0


Query: 8   FLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKSFTVGPPE 67
           F ++ LG  ++  VLKR+N   Y  KLG+K   LPPGDL WP++G+  SFL++F    PE
Sbjct: 11  FPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPE 70

Query: 68  SFIRIFTTRYGKVDMYKTHMFGRASILVCKPEICRQVLIDETKFVPSYPACMKRLFGRKS 127
           SFI+ + TRYG+  +YK HMFG   +LV  PE CR+VL D+  F   +P    +L GRKS
Sbjct: 71  SFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKS 130

Query: 128 LIRVSKAEHRKLRRMTMAPISSHAALEIYIEHTEHTVISGLEEWSSVEKPLKLLTEIKEL 187
            + +S  EH++LRR+T AP++   AL +YI+  E TV + LE+WS + + ++ L+ +++L
Sbjct: 131 FVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRKL 190

Query: 188 TFKIIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKARKRLYEILL 247
           TFK+I  IF+ S S ++    +E  Y ++  G+    IN PGF +H++LKARK+L     
Sbjct: 191 TFKVIMYIFLSSES-EHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQ 250

Query: 248 SIVNEKRLMKKSKGESWEAKDMMDLMTEVEDEDGEGMDNETITDLIFGKLFAGHETSAFT 307
           SIV  +R  +K    S   KDM+D + +V+DE+G  +D+E I DL+   L AGHE+S   
Sbjct: 251 SIVTNRRNQRKQNISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHL 310

Query: 308 TMWAIIFLTDHPHIFQRAKEEQEDIIRRRPSTQKGINLVEFKQMKFLSQVIDETLRLSSI 367
           TMWA I + +HP I Q+AKEEQE I+++R   QK + L E ++M +LSQVIDETLR+ + 
Sbjct: 311 TMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITF 370

Query: 368 AFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDDFIGNPGAF 427
           +   FREA +D+ ++G +IPKGWKV+ W R +++D E++P P+ F+PSRW+ +    G F
Sbjct: 371 SLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTF 430

Query: 428 TPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPLPHPKDKCLARVLK 487
            PFGLG  LCPG DLAKLEISIFLHHFLL Y++ER NP C + +LP   PKD CLAR+ +
Sbjct: 431 LPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 486

[Close]

BLAST of Cp4.1LG14g08880 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 449.9 bits (1156), Expect = 2.6e-126
Identity = 223/479 (46.56%), Postives = 314/479 (65.55%), Query Frame = 0


Query: 8   FLILPLGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKSFTVGPPE 67
           F ++ LG   +  +++++N   Y   LG+    LPPGDL WP +G+ LSFL++F    P+
Sbjct: 12  FPLMVLGCFGLNWLVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPD 71

Query: 68  SFIRIFTTRYGKVDMYKTHMFGRASILVCKPEICRQVLIDETKFVPSYPACMKRLFGRKS 127
           SF R    RYG   +YK HMFG  SI+V   + CR+VL D+  F P +P     L GRKS
Sbjct: 72  SFTRTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKS 131

Query: 128 LIRVSKAEHRKLRRMTMAPISSHAALEIYIEHTEHTVISGLEEWSSVEKPLKLLTEIKEL 187
            + +S  EH++LRR+T AP++ H AL  YI + E  VI+ L++W+ + +  + LT +++L
Sbjct: 132 FVGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKL 191

Query: 188 TFKIIWNIFMGSTSIDYTTKEMEALYDDVALGLFCFPINFPGFNFHKSLKARKRLYEILL 247
           TF+II  IF+ S S +     +E  Y  +  G+    +N PGF +H++LKARK L     
Sbjct: 192 TFRIIMYIFLSSES-ENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQ 251

Query: 248 SIVNEKRLMKKSKGESWEAKDMMDLMTEVEDEDGEGMDNETITDLIFGKLFAGHETSAFT 307
           SIV E+R  +K    S   KDM+D +  V+DEDG+ +D+E I D++   L AGHE+S  T
Sbjct: 252 SIVTERRNQRKQNILS-NKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHT 311

Query: 308 TMWAIIFLTDHPHIFQRAKEEQEDIIRRRPSTQKGINLVEFKQMKFLSQVIDETLRLSSI 367
            MWA +FL +HP + QRAK EQE I++ RP  QKG++L E ++M+FLSQV+DETLR+ + 
Sbjct: 312 IMWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITF 371

Query: 368 AFATFREATTDININGKLIPKGWKVILWHRGLYMDEELHPSPQDFNPSRWDD-FIGNPGA 427
           +   FREA TD+ +NG LIPKGWKV+ W R +++D E+ P P+ F+P+RWD+ F+   GA
Sbjct: 372 SLTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGA 431

Query: 428 FTPFGLGVRLCPGRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPLPHPKDKCLARV 486
           F PFG G  LCPG DLAKLEISIFLHHFLL Y+++R NP+C + YLP   P D CLAR+
Sbjct: 432 FLPFGAGSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARI 487

[Close]

BLAST of Cp4.1LG14g08880 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 239.6 bits (610), Expect = 5.4e-63
Identity = 142/446 (31.84%), Postives = 224/446 (50.22%), Query Frame = 0


Query: 41  LPPGDLSWPLLGSTLSFLKSFTVGPPESFIRIFTTRYGKVDMYKTHMFGRASILVCKPEI 100
           LPPG +  P +G TL          P SF      +YG  D++KTH+ G   +++  PE 
Sbjct: 49  LPPGSMGLPYIGETLRLYTE----NPNSFFATRQNKYG--DIFKTHILGCPCVMISSPEA 108

Query: 101 CRQVLIDETK-FVPSYPACMKRLFGRKSLIRVSKAEHRKLRRMTMAPISSHAALEIYIEH 160
            R VL+ +   F P+YP   +R+ G ++L       H  L+R+  +     +AL   + H
Sbjct: 109 ARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMP-SALRPTVSH 168

Query: 161 TEHTVISGLEEWSSVEKPLKLLTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYDDVALG 220
            E  V+  L  W+S +K +  L  +K   F +      G      T   ++ LY  +  G
Sbjct: 169 IELLVLQTLSSWTS-QKSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERG 228

Query: 221 LFCFPINFPGFNFHKSLKARKRLYEILLSIVNEKRLMKKSKGESWEAKDMMDLMTEVEDE 280
               P++ PG  FHKS+KAR  L E L  ++ ++R   + +G       ++ ++   +D+
Sbjct: 229 YNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEG------GLLGVLLGAKDQ 288

Query: 281 DGEGMDNETITDLIFGKLFAGHETSAFTTMWAIIFLTDHPHIFQRAKEEQEDIIRRRPST 340
              G+ +  I D I G +FA  +T+A    W + +L DHP++ Q    EQ  I ++    
Sbjct: 289 KRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKE 348

Query: 341 QKGINLVEFKQMKFLSQVIDETLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGL 400
            + I+  + ++M   ++VI ETLR +S+   TFREA  D+  +G LIPKGWKV+   R +
Sbjct: 349 NRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRI 408

Query: 401 YMDEELHPSPQDFNPSRWDDFIGNPGAFTPFGLGVRLCPGRDLAKLEISIFLHHFLLNYK 460
           +   E  P P+ F+PSR+ +    P  + PFG GV  CPG +LAKLE+ I LHH   +++
Sbjct: 409 HHSSEFFPDPEKFDPSRF-EVAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFR 468

Query: 461 IERYNPQCQLTYLPLPHPKDKCLARV 486
            E    +  + Y P P PK     RV
Sbjct: 469 WEVIGDEEGIQYGPFPVPKKGLPIRV 479

[Close]

BLAST of Cp4.1LG14g08880 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 239.6 bits (610), Expect = 5.4e-63
Identity = 142/446 (31.84%), Postives = 224/446 (50.22%), Query Frame = 0


Query: 41  LPPGDLSWPLLGSTLSFLKSFTVGPPESFIRIFTTRYGKVDMYKTHMFGRASILVCKPEI 100
           LPPG +  P +G TL          P SF      +YG  D++KTH+ G   +++  PE 
Sbjct: 49  LPPGSMGLPYIGETLRLYTE----NPNSFFATRQNKYG--DIFKTHILGCPCVMISSPEA 108

Query: 101 CRQVLIDETK-FVPSYPACMKRLFGRKSLIRVSKAEHRKLRRMTMAPISSHAALEIYIEH 160
            R VL+ +   F P+YP   +R+ G ++L       H  L+R+  +     +AL   + H
Sbjct: 109 ARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMP-SALRPTVSH 168

Query: 161 TEHTVISGLEEWSSVEKPLKLLTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYDDVALG 220
            E  V+  L  W+S +K +  L  +K   F +      G      T   ++ LY  +  G
Sbjct: 169 IELLVLQTLSSWTS-QKSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERG 228

Query: 221 LFCFPINFPGFNFHKSLKARKRLYEILLSIVNEKRLMKKSKGESWEAKDMMDLMTEVEDE 280
               P++ PG  FHKS+KAR  L E L  ++ ++R   + +G       ++ ++   +D+
Sbjct: 229 YNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEG------GLLGVLLGAKDQ 288

Query: 281 DGEGMDNETITDLIFGKLFAGHETSAFTTMWAIIFLTDHPHIFQRAKEEQEDIIRRRPST 340
              G+ +  I D I G +FA  +T+A    W + +L DHP++ Q    EQ  I ++    
Sbjct: 289 KRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKE 348

Query: 341 QKGINLVEFKQMKFLSQVIDETLRLSSIAFATFREATTDININGKLIPKGWKVILWHRGL 400
            + I+  + ++M   ++VI ETLR +S+   TFREA  D+  +G LIPKGWKV+   R +
Sbjct: 349 NRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRI 408

Query: 401 YMDEELHPSPQDFNPSRWDDFIGNPGAFTPFGLGVRLCPGRDLAKLEISIFLHHFLLNYK 460
           +   E  P P+ F+PSR+ +    P  + PFG GV  CPG +LAKLE+ I LHH   +++
Sbjct: 409 HHSSEFFPDPEKFDPSRF-EVAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFR 468

Query: 461 IERYNPQCQLTYLPLPHPKDKCLARV 486
            E    +  + Y P P PK     RV
Sbjct: 469 WEVIGDEEGIQYGPFPVPKKGLPIRV 479

The following BLAST results are available for this feature:

BLAST of Cp4.1LG14g08880 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5BSX1	3.4e-147	51.89	Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1	[more]
Q9C5Y2	2.0e-126	46.88	Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2	[more]
O23051	3.7e-125	46.56	Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1	[more]
Q5VRM7	1.1e-124	45.83	Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ...	[more]
Q43246	3.6e-120	44.17	Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1	[more]

BLAST of Cp4.1LG14g08880 vs. NCBI nr


Match Name	Identity	Description
XP_023552974.1	100.00	beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo]	[more]
XP_023552973.1	90.80	beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo]	[more]
XP_023007801.1	90.59	beta-amyrin 11-oxidase-like [Cucurbita maxima]	[more]
XP_022923281.1	90.80	beta-amyrin 11-oxidase-like [Cucurbita moschata]	[more]
XP_023007793.1	90.39	beta-amyrin 11-oxidase-like isoform X2 [Cucurbita maxima]	[more]

BLAST of Cp4.1LG14g08880 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1L1P3	90.59	beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111500300 PE=3 SV=...	[more]
A0A6J1E6E2	90.80	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111431022 PE=3 S...	[more]
A0A6J1L8M7	90.39	beta-amyrin 11-oxidase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1115002...	[more]
A0A6J1KZN6	89.47	beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1115002...	[more]
A0A6J1EBC2	89.16	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111431021 PE=3 S...	[more]

BLAST of Cp4.1LG14g08880 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32440.1	1.4e-127	46.88	ent-kaurenoic acid hydroxylase 2	[more]
AT2G32440.2	1.4e-127	46.88	ent-kaurenoic acid hydroxylase 2	[more]
AT1G05160.1	2.6e-126	46.56	cytochrome P450, family 88, subfamily A, polypeptide 3	[more]
AT2G29090.1	5.4e-63	31.84	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]
AT2G29090.2	5.4e-63	31.84	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 437..448 score: 40.16 coord: 299..316 score: 33.15 coord: 428..437 score: 54.72 coord: 356..367 score: 41.44
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 42..463 e-value: 4.8E-68 score: 230.0
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 395..419 score: 25.12 coord: 437..460 score: 30.6 coord: 427..437 score: 53.04 coord: 288..305 score: 24.84 coord: 308..334 score: 24.66
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 34..487 e-value: 1.0E-101 score: 342.9
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 40..485
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 19..466
None	No IPR available	PANTHER	PTHR24286:SF199	CYTOCHROME P450 88D6	coord: 19..466
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 430..439

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG14g08880.1	Cp4.1LG14g08880.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen