Cp4.1LG14g08320 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG14g08320
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description3-hydroxy-3-methylglutaryl coenzyme A synthase
LocationCp4.1LG14: 6813133 .. 6817879 (+)
RNA-Seq ExpressionCp4.1LG14g08320
SyntenyCp4.1LG14g08320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGCATTCTATTTAAGATCCGCCCCCATGTGAGCCTCACCATCTTTCTCTGCTACTTTCAAGGATTTCCTCTCTCCTCTCTCAAATTTTCTCTCTCCACTTCTTCGTTTTCACAGCAGAATCAGGTGGAGAGGAGAGTTTCATAGCATACTTTGGTTCTGTATTGCATTGGATCGGTTTTATTTTGAATTGAAGGCAAAAACCGGCTCTGGAAATGGCTAAGAATGTTGGAATTCTCGCTATTGATATCTACTTCCCTCCTACCTACATTCAGCAGGTGAGGATCTCTCAGTTTGAACTCGTTTTTAGCTTTTGTGTACTGTAGGTTGCTTTGCGGCTTGGTTTTGGATTGTATTTTGGTAGTGTTTAGAGAAATCGGTGAAGTGAGGGTAGCATTTTGTTCTTGTTCTTGTTTCGTTGAATTTGCTTTTGAATTTTGTGTTTCTTGCAAGTAATTTTGAAATTGTAGTGTTCTTCGCGATCTTAGATGATTGTTCTGTTTTTTTTTTTTCCTTTCGTTTTTTGGAATGTTTCAAATTGTTCCCCCTTTTCTTGTTTAGTTGTTTGCGATCGACGATTGATTTTGAGTTATGCATTTTCACATTTATGATTATAATGTCTGTTTGTGTGTGTGGATGAGTTGGGTCCACTCCAGCTTTTGAGCACATTTCTGGGCTGAGGGCCTACTCAGCTGTTTGGTGGCAGCTTTTGAGCTCAAATTTAGCACACATCATGTGCATGTTGTTTTTCTTTAATCGTTCTCTTAGTTGTTCTTGTTGGATCTGCATCAAGTTTTGAAAGAGAAAAGCGATTTGACATGGTTGTAGTATGAATAAAGAATTGCATCTCTTTGCATCTCTAAGTCTCACTATTTGTTCTTGCACTTACATGAATGCTTGCTACTGAAATTAATATGCCGCCTGCTTTGGCAACATAGGAAGCATTGGAAGCTCATGATGGTGCAAGCAAGGGGAAATACACTATTGGGCTTGGACAGGATTGCATGGCGTTCTGTACTGAGGTTGAAGACGTCATCTCAATGAGGTAGTGACCATATTTCATCCTGTTTTGCTAATTCTTTTGTTGGGCTAAAATCTTTGTGGCTCAGTATATGGACACAGCCGTTAGCTTCAATATCTTTATTCTGTATTTCAAAGAATCTTCTTAACATCAAAGTTCTTGGATTTTCAGCTTGACAGTTGTCACTTCCCTCCTTGAAAAGTATGGAATTGATCCAAAACAAATTGGCCGGCTAGAAGTTGGCAGTGAGACTGTTATAGATAAAAGCAAATCAATTAAGACTTTCCTGATGCAAATATTTGAGGTAAAAATTTTGAGTACTTCTGTGATGGACTTTATTTTCTCTGATAAAATAGTTGAATGAAGTATATTGTTGATCTGAACCAATTGTTCTATTTTTTTTAGTACAATAAGGGCCGATCCAGTTTGCAGATTGAAAGTTATCTTAAATAATTGTGATGATTTTCATTAGTGGATTGGTTCATTAACTTTAAAGTTCATTTAGAGAAGCTAAATCATCTAGGAGTCTTGTAAACAAATATTTTTCTATTCCAAAAGTTTCCTTTTTCTTTTTTAGTAGTTAATTTATTGATTATAAAAGAGGAGTATGGATCACTAACGTTACTTATTTTATATTTAAGCTCGATAATACTTAAATAGATAACATACGTACATATATATATATTGAAGCTGCGTAGGTCTTTTGGTACTTTTGAGGATGCAAATCTCAACTTTTGTCTGCTGATCTGTTTGGATGCAATCCTTTTCTTGATATAATAAATATTGGTTCATACTGAATGTTCTTATCTTGTCATATATGCAGAAACATGGAAACACCGACATTGAAGGTGTTGATTCAACGAATGCGTGCTATGGGGGTACAGCTGCCCTATTCAACTGTGTCAATTGGGTTGAGAGCAGCTCCTGGGATGGGCGTTATGGTCTTGTGGTGTGCACAGATAGTGCGGTATGGTATAATCTGGCCTATCTTATCATTATATTTGAATTAGCTTTGGTGTATATCTATAATATTGCAAAATAGAAGATAAGAAATCTTTGACTTTGTTCTGTTGAAATCGGCATTACTGACTTGTCTATTATGTTAGGTGTATGCTGAAGGACCTGCTCGTCCTACTGGTGGGGCTGCAGCAATTGCCATGCTGGTAGGACCTGATGCTCCTATTGGTTTTGAAAGCAAGTTGAGGGGAAGCCATATGTCTCACGTTTATGATTTTTACAAGCCTAATTTAGCGAGCGAATACCCGGTAATCTTTAACAGAATAAATTGCAATTCATTGGTTATTTCCAGCTTTTTGAAGAATTTATGGCATTTATTTAGTTTCTTGCTACAGCTAGTGAGTGATCTGTAATGAAATGCTTGCAGGTTGTTGATGGTAAACTCTCTCAAACCTGTTACCTCATGGCCTTGGATTCTTGCTACAAACAATTATGTCACAAGTAAGTTCATATTTCAAATAAATAAATCTTCCTATATGTATTTTATCTTTACCTGACATTGCACTTTTATTAGGTATGAGAAACTGGAGGGCAAGCAATTTTCCATTTCTGATGCTGACTATTTTGTATTTCATTCTCCCTATAACAAGGTAACATATGTCATAAATTGATTAAGGCTAATCTTATCACTGAAATCTCTGGATAACTTATTTGGGTATTTATGTGAGCAGCTTGTGCAGAAGAGCTTTGCTCGATTGCTCTTTAACGATTTCATGAGAAATGCCAGGTAAACGTGCTACCTCTGCTCAAATTCGAAACATCTGGTTGTTTCTGGTGGTTTTGTGGGTGTTAAAGTTTTTTCTGCTTTTCACAGCTCAATAGATGAAGTTGCTAAAGAAAAGCTGGCCCCGTTTTCAACCTTGTCGAACGACGAAACTTACCAGAGTCGGGATCTCGAAAAGGTCTTATACCTTTTAGCTGGTCATTTAGTCATCACTTTTTATTTACTAATATAATGGATTTGCAGATAACTCAACAACTTGCGAAGCCTCTTTATGATGCAAAGGTGCAACCCTCTACTTTAATACCAAAGCAAGTCGGAAATATGTATACTGCCTCTCTTTATGCAGCATTTATATCACTTCTTCACAACAAGAATAACTCACTGGTATACATTTTTCTCATTTACCTATATTATACACATCTGGCTGCTCTGCTTTCACCTGTCTAAATGGGTTGTTTCCATACCAGACAGGAAATCGGGTTGTATTGTTCTCTTATGGAAGTGGCTCAACTGCAACGATGTTCTCATTGAAGCTCAATGAAGGTCAAGCTCCCTTTAGTTTGTCCAACATTTCAGCTATCTTGAACGTGGATAAAAAGTTGAAATCGAGGCATGAGGTAATTATTTCTCTCTGAAGTTTACCATGAACTTAACTATAGATCATCTGAACAATTGTCTCGAATGTTATTGAACTAGAACTTAATTTAAGCCAACGTTACAATGGCATTTTTAGCCTGAACTGTAATGTCCTAAGCCCACCGCTCGCAGATATTGTTCTCTTTGGTCTTTCCCTCTTGGACTTCCCCTCAAAGATTTTAAAACATGACTGCTATAGAGAGGTTTCCACACTGTTATAAAAATGTTTCGTTTTCCTCCCCAACTGATGTGGGACCTCACAATCCACCCTCCTTCAGAGCCCAACGTCCTCGCTAGCACTCGTACCCTTCTCCAATCGATGTGGGACCCCTCAATCCACCCTCTTTTGGGACCCAGCGTCCTTGCTGGCACACCGCCTCGTGTCCACCCTCCTTTGGGGTTCAGCCTCCTCGCTGGCACATTGACTGGTGTCTAGCTCTAATACCATTTGTAACGCCTAAAGCCCACACTATGTCCTCTTTGGGCTTTCTCTTTCGGGCTTCCCCTCAAGATTTTTAAAACACGTCTCCTAGGGAGAGGTTTTAACACCCTTATAAAGAATGTTTCGTTCTCCTCCCCAACCAATGTGGGATCTCACATGAATGATACCTATCCAATCGTCTTGAGAGTTCAAACTTTGGACAAACATTTGGTATCCTAGAATGTATGATGTTGATATCAAGTAGGAATGGTTAAATCACCACTAAGATCAATAAACCTTCATAGATCTGTGGTTCTAAAGAGCATTTTTTATGGTTGTGCACGGTTATACTTGAGAAAGTCAAACATTAAGATACTTTAATATTAATCCATGGATTCGGACATTTAATTTTCGTGCAGCTGGTACCGGAGAAATTTGTCGAAATAATGCAGCTGATGGAGCATCGTTACGGGGCTAAGGACTTCGTGACGAGCAAGGACTGCAGCTTGCTACCAGCAGGCTCCTACTATCTCACTGAAGTTGATTCGTTATACAGGAGATTCTACGCGAAGAAGGATGGGGAAAGTGAAAAAATGGAGAACGGTGTGGTTGCTAATGGGCACTGAAATAAAGTTGAGAATAAAGAATTTTTCTTAGCACAAGTTGTTCAGAAGCTAGAGAAGAAGGCAGTACGATGAGAGCGGGTAGTTGTATGTTGTTTTGTTTTTCCTTGATGTTAGGAAAATTGTCTGAATAATGAGTTGGTTTTATTTATTCACAAGATACCATGTAATGTTGTTGGTTGTGATGATGTTATTAATGTAGACTCCTTCCCCTCTTTCTCTCTTCCATTGCTAACTATCATTACATTTGTTTCTTCATCTCTCTTCAAATTTTCAAATTAGGCCTCTGTTCTTGTGTGGCTAGTAGT

mRNA sequence

TGCATTCTATTTAAGATCCGCCCCCATGTGAGCCTCACCATCTTTCTCTGCTACTTTCAAGGATTTCCTCTCTCCTCTCTCAAATTTTCTCTCTCCACTTCTTCGTTTTCACAGCAGAATCAGGTGGAGAGGAGAGTTTCATAGCATACTTTGGTTCTGTATTGCATTGGATCGGTTTTATTTTGAATTGAAGGCAAAAACCGGCTCTGGAAATGGCTAAGAATGTTGGAATTCTCGCTATTGATATCTACTTCCCTCCTACCTACATTCAGCAGGAAGCATTGGAAGCTCATGATGGTGCAAGCAAGGGGAAATACACTATTGGGCTTGGACAGGATTGCATGGCGTTCTGTACTGAGGTTGAAGACGTCATCTCAATGAGCTTGACAGTTGTCACTTCCCTCCTTGAAAAGTATGGAATTGATCCAAAACAAATTGGCCGGCTAGAAGTTGGCAGTGAGACTGTTATAGATAAAAGCAAATCAATTAAGACTTTCCTGATGCAAATATTTGAGAAACATGGAAACACCGACATTGAAGGTGTTGATTCAACGAATGCGTGCTATGGGGGTACAGCTGCCCTATTCAACTGTGTCAATTGGGTTGAGAGCAGCTCCTGGGATGGGCGTTATGGTCTTGTGGTGTGCACAGATAGTGCGGTGTATGCTGAAGGACCTGCTCGTCCTACTGGTGGGGCTGCAGCAATTGCCATGCTGGTAGGACCTGATGCTCCTATTGGTTTTGAAAGCAAGTTGAGGGGAAGCCATATGTCTCACGTTTATGATTTTTACAAGCCTAATTTAGCGAGCGAATACCCGGTTGTTGATGGTAAACTCTCTCAAACCTGTTACCTCATGGCCTTGGATTCTTGCTACAAACAATTATGTCACAAGTATGAGAAACTGGAGGGCAAGCAATTTTCCATTTCTGATGCTGACTATTTTGTATTTCATTCTCCCTATAACAAGCTTGTGCAGAAGAGCTTTGCTCGATTGCTCTTTAACGATTTCATGAGAAATGCCAGCTCAATAGATGAAGTTGCTAAAGAAAAGCTGGCCCCGTTTTCAACCTTGTCGAACGACGAAACTTACCAGAGTCGGGATCTCGAAAAGATAACTCAACAACTTGCGAAGCCTCTTTATGATGCAAAGGTGCAACCCTCTACTTTAATACCAAAGCAAGTCGGAAATATGTATACTGCCTCTCTTTATGCAGCATTTATATCACTTCTTCACAACAAGAATAACTCACTGACAGGAAATCGGGTTGTATTGTTCTCTTATGGAAGTGGCTCAACTGCAACGATGTTCTCATTGAAGCTCAATGAAGGTCAAGCTCCCTTTAGTTTGTCCAACATTTCAGCTATCTTGAACGTGGATAAAAAGTTGAAATCGAGGCATGAGCTGGTACCGGAGAAATTTGTCGAAATAATGCAGCTGATGGAGCATCGTTACGGGGCTAAGGACTTCGTGACGAGCAAGGACTGCAGCTTGCTACCAGCAGGCTCCTACTATCTCACTGAAGTTGATTCGTTATACAGGAGATTCTACGCGAAGAAGGATGGGGAAAGTGAAAAAATGGAGAACGGTGTGGTTGCTAATGGGCACTGAAATAAAGTTGAGAATAAAGAATTTTTCTTAGCACAAGTTGTTCAGAAGCTAGAGAAGAAGGCAGTACGATGAGAGCGGGTAGTTGTATGTTGTTTTGTTTTTCCTTGATGTTAGGAAAATTGTCTGAATAATGAGTTGGTTTTATTTATTCACAAGATACCATGTAATGTTGTTGGTTGTGATGATGTTATTAATGTAGACTCCTTCCCCTCTTTCTCTCTTCCATTGCTAACTATCATTACATTTGTTTCTTCATCTCTCTTCAAATTTTCAAATTAGGCCTCTGTTCTTGTGTGGCTAGTAGT

Coding sequence (CDS)

ATGGCTAAGAATGTTGGAATTCTCGCTATTGATATCTACTTCCCTCCTACCTACATTCAGCAGGAAGCATTGGAAGCTCATGATGGTGCAAGCAAGGGGAAATACACTATTGGGCTTGGACAGGATTGCATGGCGTTCTGTACTGAGGTTGAAGACGTCATCTCAATGAGCTTGACAGTTGTCACTTCCCTCCTTGAAAAGTATGGAATTGATCCAAAACAAATTGGCCGGCTAGAAGTTGGCAGTGAGACTGTTATAGATAAAAGCAAATCAATTAAGACTTTCCTGATGCAAATATTTGAGAAACATGGAAACACCGACATTGAAGGTGTTGATTCAACGAATGCGTGCTATGGGGGTACAGCTGCCCTATTCAACTGTGTCAATTGGGTTGAGAGCAGCTCCTGGGATGGGCGTTATGGTCTTGTGGTGTGCACAGATAGTGCGGTGTATGCTGAAGGACCTGCTCGTCCTACTGGTGGGGCTGCAGCAATTGCCATGCTGGTAGGACCTGATGCTCCTATTGGTTTTGAAAGCAAGTTGAGGGGAAGCCATATGTCTCACGTTTATGATTTTTACAAGCCTAATTTAGCGAGCGAATACCCGGTTGTTGATGGTAAACTCTCTCAAACCTGTTACCTCATGGCCTTGGATTCTTGCTACAAACAATTATGTCACAAGTATGAGAAACTGGAGGGCAAGCAATTTTCCATTTCTGATGCTGACTATTTTGTATTTCATTCTCCCTATAACAAGCTTGTGCAGAAGAGCTTTGCTCGATTGCTCTTTAACGATTTCATGAGAAATGCCAGCTCAATAGATGAAGTTGCTAAAGAAAAGCTGGCCCCGTTTTCAACCTTGTCGAACGACGAAACTTACCAGAGTCGGGATCTCGAAAAGATAACTCAACAACTTGCGAAGCCTCTTTATGATGCAAAGGTGCAACCCTCTACTTTAATACCAAAGCAAGTCGGAAATATGTATACTGCCTCTCTTTATGCAGCATTTATATCACTTCTTCACAACAAGAATAACTCACTGACAGGAAATCGGGTTGTATTGTTCTCTTATGGAAGTGGCTCAACTGCAACGATGTTCTCATTGAAGCTCAATGAAGGTCAAGCTCCCTTTAGTTTGTCCAACATTTCAGCTATCTTGAACGTGGATAAAAAGTTGAAATCGAGGCATGAGCTGGTACCGGAGAAATTTGTCGAAATAATGCAGCTGATGGAGCATCGTTACGGGGCTAAGGACTTCGTGACGAGCAAGGACTGCAGCTTGCTACCAGCAGGCTCCTACTATCTCACTGAAGTTGATTCGTTATACAGGAGATTCTACGCGAAGAAGGATGGGGAAAGTGAAAAAATGGAGAACGGTGTGGTTGCTAATGGGCACTGA

Protein sequence

MAKNVGILAIDIYFPPTYIQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTVVTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGGTAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISDADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLTGNRVVLFSYGSGSTATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFVTSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH
Homology
BLAST of Cp4.1LG14g08320 vs. ExPASy Swiss-Prot
Match: P54873 (Hydroxymethylglutaryl-CoA synthase OS=Arabidopsis thaliana OX=3702 GN=HMGS PE=1 SV=2)

HSP 1 Score: 774.6 bits (1999), Expect = 6.2e-223
Identity = 377/467 (80.73%), Postives = 421/467 (90.15%), Query Frame = 0

Query: 1   MAKNVGILAIDIYFPPTYIQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV 60
           MAKNVGILA+DIYFPPT +QQEALEAHDGASKGKYTIGLGQDC+AFCTE+EDVISMS   
Sbjct: 1   MAKNVGILAMDIYFPPTCVQQEALEAHDGASKGKYTIGLGQDCLAFCTELEDVISMSFNA 60

Query: 61  VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG 120
           VTSL EKY IDP QIGRLEVGSETVIDKSKSIKTFLMQ+FEK GNTD+EGVDSTNACYGG
Sbjct: 61  VTSLFEKYKIDPNQIGRLEVGSETVIDKSKSIKTFLMQLFEKCGNTDVEGVDSTNACYGG 120

Query: 121 TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK 180
           TAAL NCVNWVES+SWDGRYGLV+CTDSAVYAEGPARPTGGAAAIAML+GPDAPI FESK
Sbjct: 121 TAALLNCVNWVESNSWDGRYGLVICTDSAVYAEGPARPTGGAAAIAMLIGPDAPIVFESK 180

Query: 181 LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISD 240
           LR SHM+HVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYK LC+K+EK+EGK+FSI+D
Sbjct: 181 LRASHMAHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKHLCNKFEKIEGKEFSIND 240

Query: 241 ADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEK 300
           ADY VFHSPYNKLVQKSFARLL+NDF+RNASSIDE AKEK  P+S+L+ DE+YQSRDLEK
Sbjct: 241 ADYIVFHSPYNKLVQKSFARLLYNDFLRNASSIDEAAKEKFTPYSSLTLDESYQSRDLEK 300

Query: 301 ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLTGNRVVLFSYGSG 360
           ++QQ+AKP YDAKVQP+TLIPK+VGNMYTASLYAAF SL+H K+N L G RVV+FSYGSG
Sbjct: 301 VSQQIAKPFYDAKVQPTTLIPKEVGNMYTASLYAAFASLIHKKHNDLAGKRVVMFSYGSG 360

Query: 361 STATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV 420
           STATMFSL+LN+ + PFS+SNI+++++V  KLK+RHE  PEKFVE M+LMEHRYGAKDFV
Sbjct: 361 STATMFSLRLNDNKPPFSISNIASVMDVGGKLKARHEYAPEKFVETMKLMEHRYGAKDFV 420

Query: 421 TSKD--CSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH 466
           T+K+    LL  G+YYL EVDSLYRRFY KK       E+G VANGH
Sbjct: 421 TTKEGIIDLLAPGTYYLKEVDSLYRRFYGKKG------EDGSVANGH 461

BLAST of Cp4.1LG14g08320 vs. ExPASy Swiss-Prot
Match: P54872 (Hydroxymethylglutaryl-CoA synthase A OS=Dictyostelium discoideum OX=44689 GN=hgsA PE=1 SV=2)

HSP 1 Score: 456.8 bits (1174), Expect = 2.9e-127
Identity = 225/444 (50.68%), Postives = 303/444 (68.24%), Query Frame = 0

Query: 3   KNVGILAIDIYFPPTYIQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTVVT 62
           +N+GI  I++YFP TY+ QE LE  DG S+GKYT+GLGQ  MAFC + ED+ S+SL  V 
Sbjct: 5   ENIGIHGIEVYFPSTYVAQEDLEKFDGVSQGKYTLGLGQTNMAFCGDREDIYSLSLNAVN 64

Query: 63  SLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGGTA 122
           +L++K+ +DP  IGRLEVG+ETVIDKSKS+KT LM +F KHGNT I+G+D+ NACYGGT+
Sbjct: 65  NLMDKFNVDPNSIGRLEVGTETVIDKSKSVKTVLMDLFAKHGNTSIDGIDTINACYGGTS 124

Query: 123 ALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESKLR 182
           AL N + W+ESS WDGR  +VV  D AVY +GPARPTGGA  +AML+GP+API FES LR
Sbjct: 125 ALHNALQWMESSYWDGRNAIVVAGDIAVYEKGPARPTGGAGVVAMLIGPNAPITFESGLR 184

Query: 183 GSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISDAD 242
           G HM +VYDFYKP++ SEYP VDGKLS +CY  A+D+CY +    +EK  GK FS+   D
Sbjct: 185 GVHMENVYDFYKPDMDSEYPRVDGKLSISCYFRAIDNCYNRYAKAFEKKYGKSFSLDQVD 244

Query: 243 YFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEKIT 302
           + +FHSPYNKLVQKSF R+L+NDF+ N    ++     L  +  +  ++TY    LEK  
Sbjct: 245 FALFHSPYNKLVQKSFGRMLYNDFLNNP---NDSRYASLEAYKNVKPEDTYFDSVLEKAL 304

Query: 303 QQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLTGNRVVLFSYGSGST 362
             + K  Y  KV P+TL+ KQ+GN Y  S Y+  +SLL  K+N L G RV+ FSYGSG  
Sbjct: 305 SAITKNDYATKVAPTTLLAKQLGNTYCGSTYSGLLSLLDEKSNDLVGKRVLTFSYGSGLA 364

Query: 363 ATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFVTS 422
           A+ FS K+ +      +++I   +++  +L  R  + PE F E + L E R+  K++V S
Sbjct: 365 ASAFSFKVEK-----PINHIVEKVDLKNRLAKRVRVEPEIFTEKLSLRETRHNLKNYVPS 424

Query: 423 KDCSLLPAGSYYLTEVDSLYRRFY 447
            + + +  GS+YL+ VD+   R Y
Sbjct: 425 DETTNMFPGSFYLSSVDNAGIRKY 440

BLAST of Cp4.1LG14g08320 vs. ExPASy Swiss-Prot
Match: P54961 (Hydroxymethylglutaryl-CoA synthase 1 OS=Blattella germanica OX=6973 GN=HMGCS-1 PE=2 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 6.0e-125
Identity = 229/451 (50.78%), Postives = 307/451 (68.07%), Query Frame = 0

Query: 4   NVGILAIDIYFPPTYIQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTVVTS 63
           +VGI+A+++ FP  Y+ Q  LE +D  S GKYT+GLGQ  M FCT+ ED+ S+ LTVV+ 
Sbjct: 5   DVGIVALELIFPSQYVDQVDLEVYDNVSAGKYTVGLGQARMGFCTDREDINSLCLTVVSR 64

Query: 64  LLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGGTAA 123
           L+E++ I   QIGRLEVG+ET++DKSKS+KT LMQ+F+   NTDIEGVD+ NACYGGT+A
Sbjct: 65  LMERWSIPYSQIGRLEVGTETLLDKSKSVKTVLMQLFK--DNTDIEGVDTVNACYGGTSA 124

Query: 124 LFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESKLRG 183
           LFN ++WVESSSWDGRY LVV  D AVYA+G ARPTGGA A+AMLVG +AP+ F+  +R 
Sbjct: 125 LFNAISWVESSSWDGRYALVVAGDIAVYAKGSARPTGGAGAVAMLVGANAPLVFDRGVRS 184

Query: 184 SHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISDADY 243
           SHM H YDFYKP+L+S YP VDGKLS  CYL ALD CY+  C K +K  G++F I   D 
Sbjct: 185 SHMQHAYDFYKPDLSSLYPTVDGKLSIQCYLSALDHCYQLYCSKIQKQLGEKFDIERLDA 244

Query: 244 FVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEKITQ 303
            +FH+PY KLVQKS ARL+ NDF+R +          L     +  ++TY  R++EK   
Sbjct: 245 VLFHAPYCKLVQKSLARLVLNDFVRASEEERTTKYSSLEALKGVKLEDTYFDREVEKAVM 304

Query: 304 QLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKN-NSLTGNRVVLFSYGSGST 363
             +K +++ K +PS L+  QVGNMYT SLY   +SLL +K+   L G RV LFSYGSG  
Sbjct: 305 TYSKNMFEEKTKPSLLLANQVGNMYTPSLYGGLVSLLVSKSAQELAGKRVALFSYGSGLA 364

Query: 364 ATMFSLKL-NEGQAPFSLSN-ISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV 423
           ++MFSL++ ++  A  SL   +S + ++  +L  RH++ PE+F + M+  EH +    + 
Sbjct: 365 SSMFSLRISSDASAKSSLQRLVSNLSHIKPQLDLRHKVSPEEFAQTMETREHNHHKAPYT 424

Query: 424 TSKDCSLLPAGSYYLTEVDSLYRRFYAKKDG 452
                 +L  G++YL  VDSLYRR Y +  G
Sbjct: 425 PEGSIDVLFPGTWYLESVDSLYRRSYKQVPG 453

BLAST of Cp4.1LG14g08320 vs. ExPASy Swiss-Prot
Match: Q5R7Z9 (Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS=Pongo abelii OX=9601 GN=HMGCS1 PE=2 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 3.9e-124
Identity = 229/455 (50.33%), Postives = 308/455 (67.69%), Query Frame = 0

Query: 3   KNVGILAIDIYFPPTYIQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTVVT 62
           K+VGI+A++IYFP  Y+ Q  LE +DG + GKYTIGLGQ  M FCT+ ED+ S+ +TVV 
Sbjct: 15  KDVGIVALEIYFPSQYVDQAELEKYDGVAAGKYTIGLGQAKMGFCTDREDINSLCMTVVQ 74

Query: 63  SLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGGTA 122
           +L+E+  +    IGRLEVG+ET+IDKSKS+KT LMQ+FE+ GNTDIEG+D+TNACYGGTA
Sbjct: 75  NLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTA 134

Query: 123 ALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESKLR 182
           A+FN VNW+ESSSWDGRY LVV  D AVYA G ARPTGG  A+A+L+GP+AP+ FE  LR
Sbjct: 135 AVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFERGLR 194

Query: 183 GSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYE---KLEG--KQFS 242
           G+HM H YDFYKP++ SEYP+VDGKLS  CYL ALD CY   C K     + EG  K F+
Sbjct: 195 GTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIRAQWQKEGNDKDFT 254

Query: 243 ISDADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRD 302
           ++D  + +FHSPY KLVQKS AR+L NDF+ + +         L  F  +  ++TY  RD
Sbjct: 255 LNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRD 314

Query: 303 LEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKN-NSLTGNRVVLFS 362
           +EK   + +  L+  K + S L+  Q GNMYT+S+Y +  S+L   +   L G R+ +FS
Sbjct: 315 VEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFS 374

Query: 363 YGSGSTATMFSLKLNEGQAPFS-LSNISAIL-NVDKKLKSRHELVPEKFVEIMQLMEHRY 422
           YGSG  AT++SLK+ +   P S L  I+A L ++  +L SR  + P+ F E M+L E  +
Sbjct: 375 YGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTH 434

Query: 423 GAKDFVTSKDCSLLPAGSYYLTEVDSLYRRFYAKK 450
              +++       L  G++YL  VD  +RR YA++
Sbjct: 435 HLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARR 469

BLAST of Cp4.1LG14g08320 vs. ExPASy Swiss-Prot
Match: Q01581 (Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS=Homo sapiens OX=9606 GN=HMGCS1 PE=1 SV=2)

HSP 1 Score: 446.0 bits (1146), Expect = 5.0e-124
Identity = 229/455 (50.33%), Postives = 307/455 (67.47%), Query Frame = 0

Query: 3   KNVGILAIDIYFPPTYIQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTVVT 62
           K+VGI+A++IYFP  Y+ Q  LE +DG   GKYTIGLGQ  M FCT+ ED+ S+ +TVV 
Sbjct: 15  KDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCMTVVQ 74

Query: 63  SLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGGTA 122
           +L+E+  +    IGRLEVG+ET+IDKSKS+KT LMQ+FE+ GNTDIEG+D+TNACYGGTA
Sbjct: 75  NLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTA 134

Query: 123 ALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESKLR 182
           A+FN VNW+ESSSWDGRY LVV  D AVYA G ARPTGG  A+A+L+GP+AP+ FE  LR
Sbjct: 135 AVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFERGLR 194

Query: 183 GSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLC---HKYEKLEG--KQFS 242
           G+HM H YDFYKP++ SEYP+VDGKLS  CYL ALD CY   C   H   + EG  K F+
Sbjct: 195 GTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFT 254

Query: 243 ISDADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRD 302
           ++D  + +FHSPY KLVQKS AR+L NDF+ + +         L  F  +  ++TY  RD
Sbjct: 255 LNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRD 314

Query: 303 LEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKN-NSLTGNRVVLFS 362
           +EK   + +  L+  K + S L+  Q GNMYT+S+Y +  S+L   +   L G R+ +FS
Sbjct: 315 VEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFS 374

Query: 363 YGSGSTATMFSLKLNEGQAPFS-LSNISAIL-NVDKKLKSRHELVPEKFVEIMQLMEHRY 422
           YGSG  AT++SLK+ +   P S L  I+A L ++  +L SR  + P+ F E M+L E  +
Sbjct: 375 YGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTH 434

Query: 423 GAKDFVTSKDCSLLPAGSYYLTEVDSLYRRFYAKK 450
              +++       L  G++YL  VD  +RR YA++
Sbjct: 435 HLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARR 469

BLAST of Cp4.1LG14g08320 vs. NCBI nr
Match: XP_023553213.1 (hydroxymethylglutaryl-CoA synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 931 bits (2406), Expect = 0.0
Identity = 465/465 (100.00%), Postives = 465/465 (100.00%), Query Frame = 0

Query: 1   MAKNVGILAIDIYFPPTYIQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV 60
           MAKNVGILAIDIYFPPTYIQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV
Sbjct: 1   MAKNVGILAIDIYFPPTYIQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV 60

Query: 61  VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG 120
           VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG
Sbjct: 61  VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG 120

Query: 121 TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK 180
           TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK
Sbjct: 121 TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK 180

Query: 181 LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISD 240
           LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISD
Sbjct: 181 LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISD 240

Query: 241 ADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEK 300
           ADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEK
Sbjct: 241 ADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEK 300

Query: 301 ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLTGNRVVLFSYGSG 360
           ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLTGNRVVLFSYGSG
Sbjct: 301 ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLTGNRVVLFSYGSG 360

Query: 361 STATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV 420
           STATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV
Sbjct: 361 STATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV 420

Query: 421 TSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH 465
           TSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH
Sbjct: 421 TSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH 465

BLAST of Cp4.1LG14g08320 vs. NCBI nr
Match: KAG6577575.1 (Hydroxymethylglutaryl-CoA synthase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015627.1 Hydroxymethylglutaryl-CoA synthase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 929 bits (2402), Expect = 0.0
Identity = 464/465 (99.78%), Postives = 465/465 (100.00%), Query Frame = 0

Query: 1   MAKNVGILAIDIYFPPTYIQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV 60
           MAKNVGILAIDIYFPPTYI+QEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV
Sbjct: 1   MAKNVGILAIDIYFPPTYIKQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV 60

Query: 61  VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG 120
           VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG
Sbjct: 61  VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG 120

Query: 121 TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK 180
           TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK
Sbjct: 121 TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK 180

Query: 181 LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISD 240
           LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISD
Sbjct: 181 LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISD 240

Query: 241 ADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEK 300
           ADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEK
Sbjct: 241 ADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEK 300

Query: 301 ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLTGNRVVLFSYGSG 360
           ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLTGNRVVLFSYGSG
Sbjct: 301 ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLTGNRVVLFSYGSG 360

Query: 361 STATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV 420
           STATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV
Sbjct: 361 STATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV 420

Query: 421 TSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH 465
           TSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH
Sbjct: 421 TSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH 465

BLAST of Cp4.1LG14g08320 vs. NCBI nr
Match: XP_022923517.1 (hydroxymethylglutaryl-CoA synthase-like [Cucurbita moschata])

HSP 1 Score: 927 bits (2397), Expect = 0.0
Identity = 463/465 (99.57%), Postives = 465/465 (100.00%), Query Frame = 0

Query: 1   MAKNVGILAIDIYFPPTYIQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV 60
           MAKNVGILAIDI+FPPTYI+QEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV
Sbjct: 1   MAKNVGILAIDIHFPPTYIKQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV 60

Query: 61  VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG 120
           VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG
Sbjct: 61  VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG 120

Query: 121 TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK 180
           TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK
Sbjct: 121 TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK 180

Query: 181 LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISD 240
           LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISD
Sbjct: 181 LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISD 240

Query: 241 ADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEK 300
           ADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEK
Sbjct: 241 ADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEK 300

Query: 301 ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLTGNRVVLFSYGSG 360
           ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLTGNRVVLFSYGSG
Sbjct: 301 ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLTGNRVVLFSYGSG 360

Query: 361 STATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV 420
           STATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV
Sbjct: 361 STATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV 420

Query: 421 TSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH 465
           TSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH
Sbjct: 421 TSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH 465

BLAST of Cp4.1LG14g08320 vs. NCBI nr
Match: XP_022965044.1 (hydroxymethylglutaryl-CoA synthase-like [Cucurbita maxima])

HSP 1 Score: 924 bits (2388), Expect = 0.0
Identity = 461/465 (99.14%), Postives = 463/465 (99.57%), Query Frame = 0

Query: 1   MAKNVGILAIDIYFPPTYIQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV 60
           MAKNVGILAIDIYFPPTYIQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV
Sbjct: 1   MAKNVGILAIDIYFPPTYIQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV 60

Query: 61  VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG 120
           VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG
Sbjct: 61  VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG 120

Query: 121 TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK 180
           TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK
Sbjct: 121 TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK 180

Query: 181 LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISD 240
           LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISD
Sbjct: 181 LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISD 240

Query: 241 ADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEK 300
           ADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEK
Sbjct: 241 ADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEK 300

Query: 301 ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLTGNRVVLFSYGSG 360
           ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHN+NNSLTGNRVVLFSYGSG
Sbjct: 301 ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNRNNSLTGNRVVLFSYGSG 360

Query: 361 STATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV 420
           STATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV
Sbjct: 361 STATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV 420

Query: 421 TSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH 465
           TSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESE +EN VVANGH
Sbjct: 421 TSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESENIENSVVANGH 465

BLAST of Cp4.1LG14g08320 vs. NCBI nr
Match: XP_038903028.1 (hydroxymethylglutaryl-CoA synthase [Benincasa hispida])

HSP 1 Score: 901 bits (2329), Expect = 0.0
Identity = 447/465 (96.13%), Postives = 455/465 (97.85%), Query Frame = 0

Query: 1   MAKNVGILAIDIYFPPTYIQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV 60
           MA NVGILA+DIYFPPTY+QQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLT 
Sbjct: 1   MANNVGILALDIYFPPTYVQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTA 60

Query: 61  VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG 120
           VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG
Sbjct: 61  VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG 120

Query: 121 TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK 180
           TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAML+GPDAPI FESK
Sbjct: 121 TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPIAFESK 180

Query: 181 LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISD 240
           LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSIS+
Sbjct: 181 LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISN 240

Query: 241 ADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEK 300
           ADYFVFHSPYNKLVQKSFARLLFNDF RNASSIDE AKEKLAPFS LSNDE+YQSRDLEK
Sbjct: 241 ADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEPAKEKLAPFSNLSNDESYQSRDLEK 300

Query: 301 ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLTGNRVVLFSYGSG 360
           ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSL GNRV+LFSYGSG
Sbjct: 301 ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLVGNRVILFSYGSG 360

Query: 361 STATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV 420
           STATMFSLKLNEGQ PFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV
Sbjct: 361 STATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV 420

Query: 421 TSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH 465
           TSKDCSLL  G+YYLTEVDSLYRRFYAKKDGESEK+ENGVVANGH
Sbjct: 421 TSKDCSLLSPGTYYLTEVDSLYRRFYAKKDGESEKIENGVVANGH 465

BLAST of Cp4.1LG14g08320 vs. ExPASy TrEMBL
Match: A0A6J1E6B9 (3-hydroxy-3-methylglutaryl coenzyme A synthase OS=Cucurbita moschata OX=3662 GN=LOC111431190 PE=3 SV=1)

HSP 1 Score: 927 bits (2397), Expect = 0.0
Identity = 463/465 (99.57%), Postives = 465/465 (100.00%), Query Frame = 0

Query: 1   MAKNVGILAIDIYFPPTYIQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV 60
           MAKNVGILAIDI+FPPTYI+QEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV
Sbjct: 1   MAKNVGILAIDIHFPPTYIKQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV 60

Query: 61  VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG 120
           VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG
Sbjct: 61  VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG 120

Query: 121 TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK 180
           TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK
Sbjct: 121 TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK 180

Query: 181 LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISD 240
           LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISD
Sbjct: 181 LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISD 240

Query: 241 ADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEK 300
           ADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEK
Sbjct: 241 ADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEK 300

Query: 301 ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLTGNRVVLFSYGSG 360
           ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLTGNRVVLFSYGSG
Sbjct: 301 ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLTGNRVVLFSYGSG 360

Query: 361 STATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV 420
           STATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV
Sbjct: 361 STATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV 420

Query: 421 TSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH 465
           TSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH
Sbjct: 421 TSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH 465

BLAST of Cp4.1LG14g08320 vs. ExPASy TrEMBL
Match: A0A6J1HPX8 (3-hydroxy-3-methylglutaryl coenzyme A synthase OS=Cucurbita maxima OX=3661 GN=LOC111465024 PE=3 SV=1)

HSP 1 Score: 924 bits (2388), Expect = 0.0
Identity = 461/465 (99.14%), Postives = 463/465 (99.57%), Query Frame = 0

Query: 1   MAKNVGILAIDIYFPPTYIQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV 60
           MAKNVGILAIDIYFPPTYIQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV
Sbjct: 1   MAKNVGILAIDIYFPPTYIQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV 60

Query: 61  VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG 120
           VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG
Sbjct: 61  VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG 120

Query: 121 TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK 180
           TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK
Sbjct: 121 TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK 180

Query: 181 LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISD 240
           LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISD
Sbjct: 181 LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISD 240

Query: 241 ADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEK 300
           ADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEK
Sbjct: 241 ADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEK 300

Query: 301 ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLTGNRVVLFSYGSG 360
           ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHN+NNSLTGNRVVLFSYGSG
Sbjct: 301 ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNRNNSLTGNRVVLFSYGSG 360

Query: 361 STATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV 420
           STATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV
Sbjct: 361 STATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV 420

Query: 421 TSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH 465
           TSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESE +EN VVANGH
Sbjct: 421 TSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESENIENSVVANGH 465

BLAST of Cp4.1LG14g08320 vs. ExPASy TrEMBL
Match: A0A1S3BKB7 (3-hydroxy-3-methylglutaryl coenzyme A synthase OS=Cucumis melo OX=3656 GN=LOC103490966 PE=3 SV=1)

HSP 1 Score: 896 bits (2316), Expect = 0.0
Identity = 446/465 (95.91%), Postives = 453/465 (97.42%), Query Frame = 0

Query: 1   MAKNVGILAIDIYFPPTYIQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV 60
           MA NVGILAIDIYFPPTY+QQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV
Sbjct: 1   MANNVGILAIDIYFPPTYVQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV 60

Query: 61  VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG 120
           V SLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG
Sbjct: 61  VNSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG 120

Query: 121 TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK 180
           TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAML+GPDAPI FESK
Sbjct: 121 TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPIAFESK 180

Query: 181 LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISD 240
           LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSIS+
Sbjct: 181 LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISN 240

Query: 241 ADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEK 300
           ADYFVFHSPYNKLVQKSFARLLFNDF RNASSID  AKEKLAPFS LSNDE+YQSRDLEK
Sbjct: 241 ADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDVAAKEKLAPFSALSNDESYQSRDLEK 300

Query: 301 ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLTGNRVVLFSYGSG 360
           ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSL GNRVVLFSYGSG
Sbjct: 301 ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLVGNRVVLFSYGSG 360

Query: 361 STATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV 420
           STATMFSLKLNEGQ PFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV
Sbjct: 361 STATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV 420

Query: 421 TSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH 465
           TSKDCSLL  G+YYLTEVDSLYRRFYAKK+G SEK+ENGVVANGH
Sbjct: 421 TSKDCSLLSTGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH 465

BLAST of Cp4.1LG14g08320 vs. ExPASy TrEMBL
Match: A0A5D3D7H1 (3-hydroxy-3-methylglutaryl coenzyme A synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00950 PE=3 SV=1)

HSP 1 Score: 896 bits (2316), Expect = 0.0
Identity = 446/465 (95.91%), Postives = 453/465 (97.42%), Query Frame = 0

Query: 1   MAKNVGILAIDIYFPPTYIQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV 60
           MA NVGILAIDIYFPPTY+QQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV
Sbjct: 1   MANNVGILAIDIYFPPTYVQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV 60

Query: 61  VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG 120
           V SLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG
Sbjct: 61  VNSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG 120

Query: 121 TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK 180
           TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAML+GPDAPI FESK
Sbjct: 121 TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPIAFESK 180

Query: 181 LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISD 240
           LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSIS+
Sbjct: 181 LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISN 240

Query: 241 ADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEK 300
           ADYFVFHSPYNKLVQKSFARLLFNDF RNASSID  AKEKLAPFS LSNDE+YQSRDLEK
Sbjct: 241 ADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDVAAKEKLAPFSALSNDESYQSRDLEK 300

Query: 301 ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLTGNRVVLFSYGSG 360
           ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSL GNRVVLFSYGSG
Sbjct: 301 ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLVGNRVVLFSYGSG 360

Query: 361 STATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV 420
           STATMFSLKLNEGQ PFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV
Sbjct: 361 STATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV 420

Query: 421 TSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH 465
           TSKDCSLL  G+YYLTEVDSLYRRFYAKK+G SEK+ENGVVANGH
Sbjct: 421 TSKDCSLLSTGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH 465

BLAST of Cp4.1LG14g08320 vs. ExPASy TrEMBL
Match: A0A0A0L5T1 (3-hydroxy-3-methylglutaryl coenzyme A synthase OS=Cucumis sativus OX=3659 GN=Csa_3G038200 PE=3 SV=1)

HSP 1 Score: 894 bits (2309), Expect = 0.0
Identity = 446/466 (95.71%), Postives = 455/466 (97.64%), Query Frame = 0

Query: 1   MAKNVGILAIDIYFPPTYIQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV 60
           MA NVGILAIDIYFPPTY+QQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV
Sbjct: 1   MANNVGILAIDIYFPPTYVQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV 60

Query: 61  VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG 120
           V SLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG
Sbjct: 61  VNSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG 120

Query: 121 TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK 180
           TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAML+GPDAPI FESK
Sbjct: 121 TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPIAFESK 180

Query: 181 LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEG-KQFSIS 240
           LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEG KQFS+S
Sbjct: 181 LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGGKQFSLS 240

Query: 241 DADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLE 300
           +ADYFVFHSPYNKLVQKSFARLLFNDF RNASSIDE AKEKLAPFSTLSNDE+YQSRDLE
Sbjct: 241 NADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEAAKEKLAPFSTLSNDESYQSRDLE 300

Query: 301 KITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLTGNRVVLFSYGS 360
           KITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKN SL GNRVVLFSYGS
Sbjct: 301 KITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNKSLVGNRVVLFSYGS 360

Query: 361 GSTATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDF 420
           GSTATMFSLKLNEGQ PFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDF
Sbjct: 361 GSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDF 420

Query: 421 VTSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH 465
           VTSKDCSLL +G+YYLTEVDSLYRRFYAKK+G SEK+ENGVVANGH
Sbjct: 421 VTSKDCSLLSSGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH 466

BLAST of Cp4.1LG14g08320 vs. TAIR 10
Match: AT4G11820.2 (hydroxymethylglutaryl-CoA synthase / HMG-CoA synthase / 3-hydroxy-3-methylglutaryl coenzyme A synthase )

HSP 1 Score: 774.6 bits (1999), Expect = 4.4e-224
Identity = 377/467 (80.73%), Postives = 421/467 (90.15%), Query Frame = 0

Query: 1   MAKNVGILAIDIYFPPTYIQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTV 60
           MAKNVGILA+DIYFPPT +QQEALEAHDGASKGKYTIGLGQDC+AFCTE+EDVISMS   
Sbjct: 1   MAKNVGILAMDIYFPPTCVQQEALEAHDGASKGKYTIGLGQDCLAFCTELEDVISMSFNA 60

Query: 61  VTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGG 120
           VTSL EKY IDP QIGRLEVGSETVIDKSKSIKTFLMQ+FEK GNTD+EGVDSTNACYGG
Sbjct: 61  VTSLFEKYKIDPNQIGRLEVGSETVIDKSKSIKTFLMQLFEKCGNTDVEGVDSTNACYGG 120

Query: 121 TAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPIGFESK 180
           TAAL NCVNWVES+SWDGRYGLV+CTDSAVYAEGPARPTGGAAAIAML+GPDAPI FESK
Sbjct: 121 TAALLNCVNWVESNSWDGRYGLVICTDSAVYAEGPARPTGGAAAIAMLIGPDAPIVFESK 180

Query: 181 LRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQFSISD 240
           LR SHM+HVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYK LC+K+EK+EGK+FSI+D
Sbjct: 181 LRASHMAHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKHLCNKFEKIEGKEFSIND 240

Query: 241 ADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQSRDLEK 300
           ADY VFHSPYNKLVQKSFARLL+NDF+RNASSIDE AKEK  P+S+L+ DE+YQSRDLEK
Sbjct: 241 ADYIVFHSPYNKLVQKSFARLLYNDFLRNASSIDEAAKEKFTPYSSLTLDESYQSRDLEK 300

Query: 301 ITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLTGNRVVLFSYGSG 360
           ++QQ+AKP YDAKVQP+TLIPK+VGNMYTASLYAAF SL+H K+N L G RVV+FSYGSG
Sbjct: 301 VSQQIAKPFYDAKVQPTTLIPKEVGNMYTASLYAAFASLIHKKHNDLAGKRVVMFSYGSG 360

Query: 361 STATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFV 420
           STATMFSL+LN+ + PFS+SNI+++++V  KLK+RHE  PEKFVE M+LMEHRYGAKDFV
Sbjct: 361 STATMFSLRLNDNKPPFSISNIASVMDVGGKLKARHEYAPEKFVETMKLMEHRYGAKDFV 420

Query: 421 TSKD--CSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH 466
           T+K+    LL  G+YYL EVDSLYRRFY KK       E+G VANGH
Sbjct: 421 TTKEGIIDLLAPGTYYLKEVDSLYRRFYGKKG------EDGSVANGH 461

BLAST of Cp4.1LG14g08320 vs. TAIR 10
Match: AT4G11820.1 (hydroxymethylglutaryl-CoA synthase / HMG-CoA synthase / 3-hydroxy-3-methylglutaryl coenzyme A synthase )

HSP 1 Score: 669.1 bits (1725), Expect = 2.6e-192
Identity = 327/412 (79.37%), Postives = 367/412 (89.08%), Query Frame = 0

Query: 56  MSLTVVTSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTN 115
           MS   VTSL EKY IDP QIGRLEVGSETVIDKSKSIKTFLMQ+FEK GNTD+EGVDSTN
Sbjct: 1   MSFNAVTSLFEKYKIDPNQIGRLEVGSETVIDKSKSIKTFLMQLFEKCGNTDVEGVDSTN 60

Query: 116 ACYGGTAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLVGPDAPI 175
           ACYGGTAAL NCVNWVES+SWDGRYGLV+CTDSAVYAEGPARPTGGAAAIAML+GPDAPI
Sbjct: 61  ACYGGTAALLNCVNWVESNSWDGRYGLVICTDSAVYAEGPARPTGGAAAIAMLIGPDAPI 120

Query: 176 GFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGKQ 235
            FESKLR SHM+HVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYK LC+K+EK+EGK+
Sbjct: 121 VFESKLRASHMAHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKHLCNKFEKIEGKE 180

Query: 236 FSISDADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFSTLSNDETYQS 295
           FSI+DADY VFHSPYNKLVQKSFARLL+NDF+RNASSIDE AKEK  P+S+L+ DE+YQS
Sbjct: 181 FSINDADYIVFHSPYNKLVQKSFARLLYNDFLRNASSIDEAAKEKFTPYSSLTLDESYQS 240

Query: 296 RDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNNSLTGNRVVLF 355
           RDLEK++QQ+AKP YDAKVQP+TLIPK+VGNMYTASLYAAF SL+H K+N L G RVV+F
Sbjct: 241 RDLEKVSQQIAKPFYDAKVQPTTLIPKEVGNMYTASLYAAFASLIHKKHNDLAGKRVVMF 300

Query: 356 SYGSGSTATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYG 415
           SYGSGSTATMFSL+LN+ + PFS+SNI+++++V  KLK+RHE  PEKFVE M+LMEHRYG
Sbjct: 301 SYGSGSTATMFSLRLNDNKPPFSISNIASVMDVGGKLKARHEYAPEKFVETMKLMEHRYG 360

Query: 416 AKDFVTSKD--CSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH 466
           AKDFVT+K+    LL  G+YYL EVDSLYRRFY KK       E+G VANGH
Sbjct: 361 AKDFVTTKEGIIDLLAPGTYYLKEVDSLYRRFYGKKG------EDGSVANGH 406

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P548736.2e-22380.73Hydroxymethylglutaryl-CoA synthase OS=Arabidopsis thaliana OX=3702 GN=HMGS PE=1 ... [more]
P548722.9e-12750.68Hydroxymethylglutaryl-CoA synthase A OS=Dictyostelium discoideum OX=44689 GN=hgs... [more]
P549616.0e-12550.78Hydroxymethylglutaryl-CoA synthase 1 OS=Blattella germanica OX=6973 GN=HMGCS-1 P... [more]
Q5R7Z93.9e-12450.33Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS=Pongo abelii OX=9601 GN=HMGCS... [more]
Q015815.0e-12450.33Hydroxymethylglutaryl-CoA synthase, cytoplasmic OS=Homo sapiens OX=9606 GN=HMGCS... [more]
Match NameE-valueIdentityDescription
XP_023553213.10.0100.00hydroxymethylglutaryl-CoA synthase-like [Cucurbita pepo subsp. pepo][more]
KAG6577575.10.099.78Hydroxymethylglutaryl-CoA synthase, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022923517.10.099.57hydroxymethylglutaryl-CoA synthase-like [Cucurbita moschata][more]
XP_022965044.10.099.14hydroxymethylglutaryl-CoA synthase-like [Cucurbita maxima][more]
XP_038903028.10.096.13hydroxymethylglutaryl-CoA synthase [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1E6B90.099.573-hydroxy-3-methylglutaryl coenzyme A synthase OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1HPX80.099.143-hydroxy-3-methylglutaryl coenzyme A synthase OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A1S3BKB70.095.913-hydroxy-3-methylglutaryl coenzyme A synthase OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A5D3D7H10.095.913-hydroxy-3-methylglutaryl coenzyme A synthase OS=Cucumis melo var. makuwa OX=11... [more]
A0A0A0L5T10.095.713-hydroxy-3-methylglutaryl coenzyme A synthase OS=Cucumis sativus OX=3659 GN=Csa... [more]
Match NameE-valueIdentityDescription
AT4G11820.24.4e-22480.73hydroxymethylglutaryl-CoA synthase / HMG-CoA synthase / 3-hydroxy-3-methylglutar... [more]
AT4G11820.12.6e-19279.37hydroxymethylglutaryl-CoA synthase / HMG-CoA synthase / 3-hydroxy-3-methylglutar... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010122Hydroxymethylglutaryl-CoA synthase, eukaryoticTIGRFAMTIGR01833TIGR01833coord: 2..449
e-value: 2.0E-218
score: 724.0
IPR013746Hydroxymethylglutaryl-coenzyme A synthase, C-terminal domainPFAMPF08540HMG_CoA_synt_Ccoord: 175..449
e-value: 2.8E-111
score: 371.7
IPR013528Hydroxymethylglutaryl-coenzyme A synthase, N-terminalPFAMPF01154HMG_CoA_synt_Ncoord: 2..174
e-value: 1.1E-81
score: 272.8
IPR016039Thiolase-likeGENE3D3.40.47.10coord: 1..450
e-value: 1.6E-192
score: 642.4
IPR016039Thiolase-likeSUPERFAMILY53901Thiolase-likecoord: 177..447
IPR016039Thiolase-likeSUPERFAMILY53901Thiolase-likecoord: 4..172
NoneNo IPR availablePANTHERPTHR433233-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASEcoord: 1..449
NoneNo IPR availableCDDcd00827init_cond_enzymescoord: 4..368
e-value: 1.41649E-88
score: 271.614
IPR000590Hydroxymethylglutaryl-coenzyme A synthase, active sitePROSITEPS01226HMG_COA_SYNTHASEcoord: 105..120

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG14g08320.1Cp4.1LG14g08320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006084 acetyl-CoA metabolic process
biological_process GO:0010142 farnesyl diphosphate biosynthetic process, mevalonate pathway
biological_process GO:0016126 sterol biosynthetic process
biological_process GO:0008299 isoprenoid biosynthetic process
molecular_function GO:0004421 hydroxymethylglutaryl-CoA synthase activity
molecular_function GO:0016746 acyltransferase activity