Cp4.1LG14g07940 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG14g07940
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionProtein TIC 20
LocationCp4.1LG14: 6211978 .. 6212637 (-)
RNA-Seq ExpressionCp4.1LG14g07940
SyntenyCp4.1LG14g07940
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGACGCTTATCTTAAATCTCTCCCCCAAAACCCTCCATTCTTCCTTCTTCCCCTCGCCATTTCCCTCTCTCTCAAACCCTAGAACTACGGCAACAACAACCCTCACTAACCAAATCAATTTCACCACTCTACGACGACGGCGACGACGACGCATCCTCACCGTCCGATCCTCCGGCGACCGCGACAACAACACCGCCGACGGCCCCGACCGCCTCATCTCTGCCATCTGCTACTTCTACCCGTTCTTCGACGGCGTCCAGTACGGCAAGTACGTTATCACTCAGTACGCTCCCTTTCAGGTTCTGATTCAGCCCTTGGTTCCTGCGATTCGAGTTTTCAAGAGCTTTCCATTCAACGGATTTCTGGTGTTTTTGACTCTATACTTCGTCGTGGTTAGAAACCCTAATTTCAGTCGCTATGTTAGGTTCAATATTATGCAAGCCATTGTTCTTGATGTGCTTTTGATTTTCCCCGATCTTCTGGAGAGGAGCTTCAATCCGAGAGGGGGATTGGGGTTGGATTTGGTGATGAGCTTGGATAGCACTGTGTTTCTGTTTCTTTTGATTTGTTTGATTTATGGGTCATCTTCTTGCTTGTTGGGGCAGGTCCCTAGATTGCCCATTGTTGCTGATGCTGCTGATAGGCAAGTTATGTGA

mRNA sequence

ATGGCGACGCTTATCTTAAATCTCTCCCCCAAAACCCTCCATTCTTCCTTCTTCCCCTCGCCATTTCCCTCTCTCTCAAACCCTAGAACTACGGCAACAACAACCCTCACTAACCAAATCAATTTCACCACTCTACGACGACGGCGACGACGACGCATCCTCACCGTCCGATCCTCCGGCGACCGCGACAACAACACCGCCGACGGCCCCGACCGCCTCATCTCTGCCATCTGCTACTTCTACCCGTTCTTCGACGGCGTCCAGTACGGCAAGTACGTTATCACTCAGTACGCTCCCTTTCAGGTTCTGATTCAGCCCTTGGTTCCTGCGATTCGAGTTTTCAAGAGCTTTCCATTCAACGGATTTCTGGTGTTTTTGACTCTATACTTCGTCGTGGTTAGAAACCCTAATTTCAGTCGCTATGTTAGGTTCAATATTATGCAAGCCATTGTTCTTGATGTGCTTTTGATTTTCCCCGATCTTCTGGAGAGGAGCTTCAATCCGAGAGGGGGATTGGGGTTGGATTTGGTGATGAGCTTGGATAGCACTGTGTTTCTGTTTCTTTTGATTTGTTTGATTTATGGGTCATCTTCTTGCTTGTTGGGGCAGGTCCCTAGATTGCCCATTGTTGCTGATGCTGCTGATAGGCAAGTTATGTGA

Coding sequence (CDS)

ATGGCGACGCTTATCTTAAATCTCTCCCCCAAAACCCTCCATTCTTCCTTCTTCCCCTCGCCATTTCCCTCTCTCTCAAACCCTAGAACTACGGCAACAACAACCCTCACTAACCAAATCAATTTCACCACTCTACGACGACGGCGACGACGACGCATCCTCACCGTCCGATCCTCCGGCGACCGCGACAACAACACCGCCGACGGCCCCGACCGCCTCATCTCTGCCATCTGCTACTTCTACCCGTTCTTCGACGGCGTCCAGTACGGCAAGTACGTTATCACTCAGTACGCTCCCTTTCAGGTTCTGATTCAGCCCTTGGTTCCTGCGATTCGAGTTTTCAAGAGCTTTCCATTCAACGGATTTCTGGTGTTTTTGACTCTATACTTCGTCGTGGTTAGAAACCCTAATTTCAGTCGCTATGTTAGGTTCAATATTATGCAAGCCATTGTTCTTGATGTGCTTTTGATTTTCCCCGATCTTCTGGAGAGGAGCTTCAATCCGAGAGGGGGATTGGGGTTGGATTTGGTGATGAGCTTGGATAGCACTGTGTTTCTGTTTCTTTTGATTTGTTTGATTTATGGGTCATCTTCTTGCTTGTTGGGGCAGGTCCCTAGATTGCCCATTGTTGCTGATGCTGCTGATAGGCAAGTTATGTGA

Protein sequence

MATLILNLSPKTLHSSFFPSPFPSLSNPRTTATTTLTNQINFTTLRRRRRRRILTVRSSGDRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPFNGFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSLDSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM
Homology
BLAST of Cp4.1LG14g07940 vs. ExPASy Swiss-Prot
Match: Q9FM67 (Protein TIC 20-v, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-V PE=2 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 5.8e-70
Identity = 145/214 (67.76%), Postives = 166/214 (77.57%), Query Frame = 0

Query: 15  SSFFPSPFPSLSNPRTTATTTLTNQ----INFTT-----LRRRRRRRILTVRSSGDRDNN 74
           S FF +P PSL     T T TLT +    +N  T        R R   L ++S GD   +
Sbjct: 5   SQFF-APLPSL-----TGTLTLTGRSFLPLNLDTQFPKPRLSRDRAATLVLQSKGD---D 64

Query: 75  TADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPFNGFLVF 134
           + D  DR+ISA+CYFYPFFDG+QYGK++ITQY PFQ+LIQPL PAIR FKSFPFNGFL+F
Sbjct: 65  SVDASDRIISAVCYFYPFFDGIQYGKFIITQYQPFQILIQPLFPAIRAFKSFPFNGFLIF 124

Query: 135 LTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSLDSTVF 194
           +TLYFVVVRNPNFSRYVRFN MQAIVLDVLLIFPDLLERSFNPR G GLD+VMSLDSTVF
Sbjct: 125 ITLYFVVVRNPNFSRYVRFNTMQAIVLDVLLIFPDLLERSFNPRDGFGLDVVMSLDSTVF 184

Query: 195 LFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM 220
           LFLL+ LIYG S+CL G  PRLP+VA+AADRQV+
Sbjct: 185 LFLLVSLIYGFSACLFGLTPRLPLVAEAADRQVL 209

BLAST of Cp4.1LG14g07940 vs. ExPASy Swiss-Prot
Match: O82251 (Protein TIC 20-II, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-II PE=2 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 2.2e-29
Identity = 87/221 (39.37%), Postives = 122/221 (55.20%), Query Frame = 0

Query: 1   MATLILNLSPKTLHSSFFPSPFP-SLSNPRTTATTTLTNQINFTTLRRRRRRRILTVRSS 60
           MA+L L+L     +    P   P SLS P ++          F+     RR   LT R+S
Sbjct: 1   MASLCLSLHQTLTNPLSAPRCRPLSLSFPGSS---------TFSIRPSSRRATALTTRAS 60

Query: 61  GDRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPF 120
                      +R+IS   Y  PFF+ +QYG+++  QY    +L +P+ P + +++S P+
Sbjct: 61  ----YTPTPATERVISIASYALPFFNSLQYGRFLFAQYPRLGLLFEPIFPILNLYRSVPY 120

Query: 121 NGFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNP--RGGLGLDLV 180
             F+ F  LY  VVRN +FSRYVRFN MQA+ LDVLL  P LL R  +P   GG G+  +
Sbjct: 121 ASFVAFFGLYLGVVRNTSFSRYVRFNAMQAVTLDVLLAVPVLLTRILDPGQGGGFGMKAM 180

Query: 181 MSLDSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQV 219
           M   + VF+F  +C +YG  S LLG+ P +P VADAA RQ+
Sbjct: 181 MWGHTGVFVFSFMCFVYGVVSSLLGKTPYIPFVADAAGRQL 208

BLAST of Cp4.1LG14g07940 vs. ExPASy Swiss-Prot
Match: P73387 (Tic20 family protein OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=sll1737 PE=3 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 2.0e-22
Identity = 57/156 (36.54%), Postives = 92/156 (58.97%), Query Frame = 0

Query: 64  NNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPFNGFL 123
           N+TADG DR  SA+ Y  P  D   +G +++ Q+   Q++  P++P ++ +  FPF  F+
Sbjct: 4   NSTADGKDRFFSALIYVIPLIDAFMFGGFLLQQFPVLQIIYLPIMPLLQFYYQFPFASFI 63

Query: 124 VFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLL-IFPDLLERSFNPRGGLGLDLVMSLDS 183
           +F+ L+  VVRN N S ++RFN MQAI++ +LL +F  ++     P  G GL +  +L +
Sbjct: 64  IFIVLFMAVVRNNNISHFIRFNAMQAILIGILLSLFGLIVAYVIQPVFGQGL-VTETLYN 123

Query: 184 TVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQV 219
             FL  L C  +G    +LG+   +P ++DAA  QV
Sbjct: 124 FAFLGALACGFFGIVQSVLGRYAEIPTISDAAYSQV 158

BLAST of Cp4.1LG14g07940 vs. ExPASy Swiss-Prot
Match: O19916 (Tic20 family protein Ycf60 OS=Cyanidium caldarium OX=2771 GN=ycf60 PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 1.2e-06
Identity = 42/149 (28.19%), Postives = 76/149 (51.01%), Query Frame = 0

Query: 72  RLISAICYFYPFFDGV-QYGKYVITQYAPFQVLIQPLVPAIRV-FKSFPFNGFLVFLTLY 131
           R IS + YF P  +G+ Q+G   I  ++  +++ +  +  I V +   P  GF +F+TLY
Sbjct: 53  RAISCLIYFLPLLEGIAQFGIVCIDDHSWIRIIYKNTLAYIVVPYLESPLIGFCIFITLY 112

Query: 132 FVVVRN-PNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSLDSTVFLFL 191
            + VR     S++++F+I+QA++L +L      +  S     G    +   L  T+ L  
Sbjct: 113 LIFVRGIIQISKFIKFHIVQALLLYLLDSVIGTVLTSLPLEIGYSF-IGDRLADTLLLIT 172

Query: 192 LICLIYGSSSCLLGQVPRLPIVADAADRQ 218
            +  IY  +  L GQ   +P++++AA  Q
Sbjct: 173 FMISIYAGTDALKGQYSNIPLISEAAKMQ 200

BLAST of Cp4.1LG14g07940 vs. NCBI nr
Match: XP_023553383.1 (protein TIC 20-v, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 424 bits (1089), Expect = 5.91e-149
Identity = 219/219 (100.00%), Postives = 219/219 (100.00%), Query Frame = 0

Query: 1   MATLILNLSPKTLHSSFFPSPFPSLSNPRTTATTTLTNQINFTTLRRRRRRRILTVRSSG 60
           MATLILNLSPKTLHSSFFPSPFPSLSNPRTTATTTLTNQINFTTLRRRRRRRILTVRSSG
Sbjct: 22  MATLILNLSPKTLHSSFFPSPFPSLSNPRTTATTTLTNQINFTTLRRRRRRRILTVRSSG 81

Query: 61  DRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPFN 120
           DRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPFN
Sbjct: 82  DRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPFN 141

Query: 121 GFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSL 180
           GFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSL
Sbjct: 142 GFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSL 201

Query: 181 DSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM 219
           DSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM
Sbjct: 202 DSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM 240

BLAST of Cp4.1LG14g07940 vs. NCBI nr
Match: XP_022965178.1 (protein TIC 20-v, chloroplastic [Cucurbita maxima])

HSP 1 Score: 421 bits (1081), Expect = 4.60e-148
Identity = 218/219 (99.54%), Postives = 218/219 (99.54%), Query Frame = 0

Query: 1   MATLILNLSPKTLHSSFFPSPFPSLSNPRTTATTTLTNQINFTTLRRRRRRRILTVRSSG 60
           MATLILNLSPKTL SSFFPSPFPSLSNPRTTATTTLTNQINFTTLRRRRRRRILTVRSSG
Sbjct: 1   MATLILNLSPKTLRSSFFPSPFPSLSNPRTTATTTLTNQINFTTLRRRRRRRILTVRSSG 60

Query: 61  DRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPFN 120
           DRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPFN
Sbjct: 61  DRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPFN 120

Query: 121 GFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSL 180
           GFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSL
Sbjct: 121 GFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSL 180

Query: 181 DSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM 219
           DSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM
Sbjct: 181 DSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM 219

BLAST of Cp4.1LG14g07940 vs. NCBI nr
Match: XP_022932396.1 (protein TIC 20-v, chloroplastic [Cucurbita moschata])

HSP 1 Score: 419 bits (1077), Expect = 4.28e-147
Identity = 219/220 (99.55%), Postives = 219/220 (99.55%), Query Frame = 0

Query: 1   MATLILNLSPKTLHSSFFPSPFPSLSNPRTTA-TTTLTNQINFTTLRRRRRRRILTVRSS 60
           MATLILNLSPKTLHSSFFPSPFPSLSNPRTTA TTTLTNQINFTTLRRRRRRRILTVRSS
Sbjct: 23  MATLILNLSPKTLHSSFFPSPFPSLSNPRTTAATTTLTNQINFTTLRRRRRRRILTVRSS 82

Query: 61  GDRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPF 120
           GDRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPF
Sbjct: 83  GDRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPF 142

Query: 121 NGFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMS 180
           NGFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMS
Sbjct: 143 NGFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMS 202

Query: 181 LDSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM 219
           LDSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM
Sbjct: 203 LDSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM 242

BLAST of Cp4.1LG14g07940 vs. NCBI nr
Match: KAG6577521.1 (Protein TIC 20-v, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015580.1 Protein TIC 20-v, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 415 bits (1066), Expect = 9.23e-146
Identity = 217/220 (98.64%), Postives = 217/220 (98.64%), Query Frame = 0

Query: 1   MATLILNLSPKTLHSSFFPSPFPSLSNPRTTATTT-LTNQINFTTLRRRRRRRILTVRSS 60
           MA LILNLSPKTLHSSFFPSPFPSL NPRTTATTT LTNQINFTTLRRRRRRRILTVRSS
Sbjct: 1   MAKLILNLSPKTLHSSFFPSPFPSLPNPRTTATTTTLTNQINFTTLRRRRRRRILTVRSS 60

Query: 61  GDRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPF 120
           GDRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPF
Sbjct: 61  GDRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPF 120

Query: 121 NGFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMS 180
           NGFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMS
Sbjct: 121 NGFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMS 180

Query: 181 LDSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM 219
           LDSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM
Sbjct: 181 LDSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM 220

BLAST of Cp4.1LG14g07940 vs. NCBI nr
Match: XP_008449034.1 (PREDICTED: protein TIC 20-v, chloroplastic [Cucumis melo] >KAA0065731.1 protein TIC 20-v [Cucumis melo var. makuwa] >TYK13757.1 protein TIC 20-v [Cucumis melo var. makuwa])

HSP 1 Score: 372 bits (955), Expect = 6.40e-129
Identity = 195/222 (87.84%), Postives = 203/222 (91.44%), Query Frame = 0

Query: 1   MATLILNLSPKTLHSSFFPSPFPSLSNPRTTATTTLTNQINFTTLRRRR---RRRILTVR 60
           MA LILNLSPKTLHSSFFPSPFP+LSNP+TT        +NFTT  RRR   RRRI TVR
Sbjct: 1   MAFLILNLSPKTLHSSFFPSPFPALSNPKTTT-------LNFTTTLRRRGRGRRRIFTVR 60

Query: 61  SSGDRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSF 120
           S+GD +NN+ADGPDRLISAICYFYPFFDGVQYGKYVITQY PFQVLIQPLVPAIRVFKSF
Sbjct: 61  STGDNNNNSADGPDRLISAICYFYPFFDGVQYGKYVITQYTPFQVLIQPLVPAIRVFKSF 120

Query: 121 PFNGFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLV 180
           P N FLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGG+GLDLV
Sbjct: 121 PLNSFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGIGLDLV 180

Query: 181 MSLDSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM 219
           MSLDSTVFLFLLICLIYGSSSCLLGQ+PRLPIVADAADRQVM
Sbjct: 181 MSLDSTVFLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM 215

BLAST of Cp4.1LG14g07940 vs. ExPASy TrEMBL
Match: A0A6J1HN42 (Protein TIC 20 OS=Cucurbita maxima OX=3661 GN=LOC111465112 PE=3 SV=1)

HSP 1 Score: 421 bits (1081), Expect = 2.23e-148
Identity = 218/219 (99.54%), Postives = 218/219 (99.54%), Query Frame = 0

Query: 1   MATLILNLSPKTLHSSFFPSPFPSLSNPRTTATTTLTNQINFTTLRRRRRRRILTVRSSG 60
           MATLILNLSPKTL SSFFPSPFPSLSNPRTTATTTLTNQINFTTLRRRRRRRILTVRSSG
Sbjct: 1   MATLILNLSPKTLRSSFFPSPFPSLSNPRTTATTTLTNQINFTTLRRRRRRRILTVRSSG 60

Query: 61  DRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPFN 120
           DRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPFN
Sbjct: 61  DRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPFN 120

Query: 121 GFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSL 180
           GFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSL
Sbjct: 121 GFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSL 180

Query: 181 DSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM 219
           DSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM
Sbjct: 181 DSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM 219

BLAST of Cp4.1LG14g07940 vs. ExPASy TrEMBL
Match: A0A6J1EW92 (Protein TIC 20 OS=Cucurbita moschata OX=3662 GN=LOC111438760 PE=3 SV=1)

HSP 1 Score: 419 bits (1077), Expect = 2.07e-147
Identity = 219/220 (99.55%), Postives = 219/220 (99.55%), Query Frame = 0

Query: 1   MATLILNLSPKTLHSSFFPSPFPSLSNPRTTA-TTTLTNQINFTTLRRRRRRRILTVRSS 60
           MATLILNLSPKTLHSSFFPSPFPSLSNPRTTA TTTLTNQINFTTLRRRRRRRILTVRSS
Sbjct: 23  MATLILNLSPKTLHSSFFPSPFPSLSNPRTTAATTTLTNQINFTTLRRRRRRRILTVRSS 82

Query: 61  GDRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPF 120
           GDRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPF
Sbjct: 83  GDRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPF 142

Query: 121 NGFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMS 180
           NGFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMS
Sbjct: 143 NGFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMS 202

Query: 181 LDSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM 219
           LDSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM
Sbjct: 203 LDSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM 242

BLAST of Cp4.1LG14g07940 vs. ExPASy TrEMBL
Match: A0A5D3CPD4 (Protein TIC 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold488G00160 PE=3 SV=1)

HSP 1 Score: 372 bits (955), Expect = 3.10e-129
Identity = 195/222 (87.84%), Postives = 203/222 (91.44%), Query Frame = 0

Query: 1   MATLILNLSPKTLHSSFFPSPFPSLSNPRTTATTTLTNQINFTTLRRRR---RRRILTVR 60
           MA LILNLSPKTLHSSFFPSPFP+LSNP+TT        +NFTT  RRR   RRRI TVR
Sbjct: 1   MAFLILNLSPKTLHSSFFPSPFPALSNPKTTT-------LNFTTTLRRRGRGRRRIFTVR 60

Query: 61  SSGDRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSF 120
           S+GD +NN+ADGPDRLISAICYFYPFFDGVQYGKYVITQY PFQVLIQPLVPAIRVFKSF
Sbjct: 61  STGDNNNNSADGPDRLISAICYFYPFFDGVQYGKYVITQYTPFQVLIQPLVPAIRVFKSF 120

Query: 121 PFNGFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLV 180
           P N FLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGG+GLDLV
Sbjct: 121 PLNSFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGIGLDLV 180

Query: 181 MSLDSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM 219
           MSLDSTVFLFLLICLIYGSSSCLLGQ+PRLPIVADAADRQVM
Sbjct: 181 MSLDSTVFLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM 215

BLAST of Cp4.1LG14g07940 vs. ExPASy TrEMBL
Match: A0A1S3BM16 (Protein TIC 20 OS=Cucumis melo OX=3656 GN=LOC103491027 PE=3 SV=1)

HSP 1 Score: 372 bits (955), Expect = 3.10e-129
Identity = 195/222 (87.84%), Postives = 203/222 (91.44%), Query Frame = 0

Query: 1   MATLILNLSPKTLHSSFFPSPFPSLSNPRTTATTTLTNQINFTTLRRRR---RRRILTVR 60
           MA LILNLSPKTLHSSFFPSPFP+LSNP+TT        +NFTT  RRR   RRRI TVR
Sbjct: 1   MAFLILNLSPKTLHSSFFPSPFPALSNPKTTT-------LNFTTTLRRRGRGRRRIFTVR 60

Query: 61  SSGDRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSF 120
           S+GD +NN+ADGPDRLISAICYFYPFFDGVQYGKYVITQY PFQVLIQPLVPAIRVFKSF
Sbjct: 61  STGDNNNNSADGPDRLISAICYFYPFFDGVQYGKYVITQYTPFQVLIQPLVPAIRVFKSF 120

Query: 121 PFNGFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLV 180
           P N FLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGG+GLDLV
Sbjct: 121 PLNSFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGIGLDLV 180

Query: 181 MSLDSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM 219
           MSLDSTVFLFLLICLIYGSSSCLLGQ+PRLPIVADAADRQVM
Sbjct: 181 MSLDSTVFLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM 215

BLAST of Cp4.1LG14g07940 vs. ExPASy TrEMBL
Match: A0A0A0L632 (Protein TIC 20 OS=Cucumis sativus OX=3659 GN=Csa_3G047830 PE=3 SV=1)

HSP 1 Score: 343 bits (881), Expect = 5.24e-118
Identity = 189/223 (84.75%), Postives = 195/223 (87.44%), Query Frame = 0

Query: 1   MATLILNLSPKTLHSSFFPSPFPSLSNPRTTATTTLTNQINFTTLRRRR---RRRILTVR 60
           MA LILNLSPKT HS FFPS    LSNP+TT        +NFTT   RR   R RILTVR
Sbjct: 1   MAFLILNLSPKTPHSPFFPS----LSNPKTTT-------LNFTTTLPRRWLARPRILTVR 60

Query: 61  SSGD-RDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKS 120
           S+GD  +NN+AD PDRLISAICYFYPFFDGVQYGKYVITQY PFQVLIQPLVPAIRVFKS
Sbjct: 61  STGDDNNNNSADAPDRLISAICYFYPFFDGVQYGKYVITQYTPFQVLIQPLVPAIRVFKS 120

Query: 121 FPFNGFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDL 180
           FP N FLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDL
Sbjct: 121 FPLNSFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDL 180

Query: 181 VMSLDSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM 219
           VMSLDSTVFLFLLICLIYGSSSCLLGQ+PRLPIVADAADRQVM
Sbjct: 181 VMSLDSTVFLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM 212

BLAST of Cp4.1LG14g07940 vs. TAIR 10
Match: AT5G55710.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast thylakoid membrane, chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: Uncharacterised conserved protein ycf60 (TAIR:AT2G47840.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 265.4 bits (677), Expect = 4.1e-71
Identity = 145/214 (67.76%), Postives = 166/214 (77.57%), Query Frame = 0

Query: 15  SSFFPSPFPSLSNPRTTATTTLTNQ----INFTT-----LRRRRRRRILTVRSSGDRDNN 74
           S FF +P PSL     T T TLT +    +N  T        R R   L ++S GD   +
Sbjct: 5   SQFF-APLPSL-----TGTLTLTGRSFLPLNLDTQFPKPRLSRDRAATLVLQSKGD---D 64

Query: 75  TADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPFNGFLVF 134
           + D  DR+ISA+CYFYPFFDG+QYGK++ITQY PFQ+LIQPL PAIR FKSFPFNGFL+F
Sbjct: 65  SVDASDRIISAVCYFYPFFDGIQYGKFIITQYQPFQILIQPLFPAIRAFKSFPFNGFLIF 124

Query: 135 LTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSLDSTVF 194
           +TLYFVVVRNPNFSRYVRFN MQAIVLDVLLIFPDLLERSFNPR G GLD+VMSLDSTVF
Sbjct: 125 ITLYFVVVRNPNFSRYVRFNTMQAIVLDVLLIFPDLLERSFNPRDGFGLDVVMSLDSTVF 184

Query: 195 LFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM 220
           LFLL+ LIYG S+CL G  PRLP+VA+AADRQV+
Sbjct: 185 LFLLVSLIYGFSACLFGLTPRLPLVAEAADRQVL 209

BLAST of Cp4.1LG14g07940 vs. TAIR 10
Match: AT2G47840.1 (Uncharacterised conserved protein ycf60 )

HSP 1 Score: 130.6 bits (327), Expect = 1.6e-30
Identity = 87/221 (39.37%), Postives = 122/221 (55.20%), Query Frame = 0

Query: 1   MATLILNLSPKTLHSSFFPSPFP-SLSNPRTTATTTLTNQINFTTLRRRRRRRILTVRSS 60
           MA+L L+L     +    P   P SLS P ++          F+     RR   LT R+S
Sbjct: 1   MASLCLSLHQTLTNPLSAPRCRPLSLSFPGSS---------TFSIRPSSRRATALTTRAS 60

Query: 61  GDRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPF 120
                      +R+IS   Y  PFF+ +QYG+++  QY    +L +P+ P + +++S P+
Sbjct: 61  ----YTPTPATERVISIASYALPFFNSLQYGRFLFAQYPRLGLLFEPIFPILNLYRSVPY 120

Query: 121 NGFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNP--RGGLGLDLV 180
             F+ F  LY  VVRN +FSRYVRFN MQA+ LDVLL  P LL R  +P   GG G+  +
Sbjct: 121 ASFVAFFGLYLGVVRNTSFSRYVRFNAMQAVTLDVLLAVPVLLTRILDPGQGGGFGMKAM 180

Query: 181 MSLDSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQV 219
           M   + VF+F  +C +YG  S LLG+ P +P VADAA RQ+
Sbjct: 181 MWGHTGVFVFSFMCFVYGVVSSLLGKTPYIPFVADAAGRQL 208

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FM675.8e-7067.76Protein TIC 20-v, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-V PE=2 ... [more]
O822512.2e-2939.37Protein TIC 20-II, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-II PE=... [more]
P733872.0e-2236.54Tic20 family protein OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 ... [more]
O199161.2e-0628.19Tic20 family protein Ycf60 OS=Cyanidium caldarium OX=2771 GN=ycf60 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_023553383.15.91e-149100.00protein TIC 20-v, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_022965178.14.60e-14899.54protein TIC 20-v, chloroplastic [Cucurbita maxima][more]
XP_022932396.14.28e-14799.55protein TIC 20-v, chloroplastic [Cucurbita moschata][more]
KAG6577521.19.23e-14698.64Protein TIC 20-v, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia]... [more]
XP_008449034.16.40e-12987.84PREDICTED: protein TIC 20-v, chloroplastic [Cucumis melo] >KAA0065731.1 protein ... [more]
Match NameE-valueIdentityDescription
A0A6J1HN422.23e-14899.54Protein TIC 20 OS=Cucurbita maxima OX=3661 GN=LOC111465112 PE=3 SV=1[more]
A0A6J1EW922.07e-14799.55Protein TIC 20 OS=Cucurbita moschata OX=3662 GN=LOC111438760 PE=3 SV=1[more]
A0A5D3CPD43.10e-12987.84Protein TIC 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold488G00160... [more]
A0A1S3BM163.10e-12987.84Protein TIC 20 OS=Cucumis melo OX=3656 GN=LOC103491027 PE=3 SV=1[more]
A0A0A0L6325.24e-11884.75Protein TIC 20 OS=Cucumis sativus OX=3659 GN=Csa_3G047830 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G55710.14.1e-7167.76FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT2G47840.11.6e-3039.37Uncharacterised conserved protein ycf60 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005691Chloroplast protein import component Tic20PFAMPF16166TIC20coord: 72..214
e-value: 1.1E-54
score: 184.9
IPR005691Chloroplast protein import component Tic20PANTHERPTHR33510PROTEIN TIC 20-II, CHLOROPLASTICcoord: 30..219
NoneNo IPR availablePANTHERPTHR33510:SF11PROTEIN TIC 20-V, CHLOROPLASTICcoord: 30..219

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG14g07940.1Cp4.1LG14g07940.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0009706 chloroplast inner membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009579 thylakoid