Cp4.1LG14g07500 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG14g07500 vs. ExPASy Swiss-Prot
Match: Q9FJ79 (DNA topoisomerase 1 beta OS=Arabidopsis thaliana OX=3702 GN=TOP1B PE=1 SV=1)

HSP 1 Score: 1026.9 bits (2654), Expect = 1.3e-298
Identity = 569/944 (60.28%), Postives = 689/944 (72.99%), Query Frame = 0

Query: 1   MAVETFGKAPM------FDDDDEDNMPIVFKRSS-----ASRQNQLNSEVKHSQSQRLNQ 60
           MA E F K  +      ++D+DED++P+VFKR+S      +R + +N+ +++S       
Sbjct: 1   MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKRNSNTAATTNRPSPINNAMRNSAI----- 60

Query: 61  LSGRPGSDACSANGQS--SNAQKRMTSLPKTSPMRPEI-GSQKASKSSIQEPLVKSLGTT 120
                GS   S   +S  ++  +  +S  ++S M+P +  S    +S+++ PL       
Sbjct: 61  -----GSTKSSPPMRSPLTSPNRSASSSTRSSMMKPALPSSSSVQRSTLKSPLRDDRSVV 120

Query: 121 LKVSDSMDSRPKQLANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPT- 180
            K  +     P                   D+ DSEDDKPLS RLK +S   +K P  + 
Sbjct: 121 AKERNGFGKAPSV--------------SKSDDEDSEDDKPLSARLKLDSKEVTKQPSSSG 180

Query: 181 -------VQKNKIKKSSEDS-------DDEVPLSSKFQMKSASGVLGGRQHGFDEKKPLD 240
                  VQK+ ++             D+  P+SS  Q K++ G    +    ++K+PL 
Sbjct: 181 RGSTQQAVQKSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLV 240

Query: 241 SKVHQNG---STSRDKLQKPAVVSSKRPLPNADSHSSSQISSKKPKLS------------ 300
           + + +NG    T     Q PA    KRPL      SS+Q S K+PKLS            
Sbjct: 241 NNIDRNGLKPKTEGHSSQAPA----KRPLEKGS--SSNQSSVKRPKLSEPARPVKVEQGS 300

Query: 301 -------------DSSTPLSTNTVSLKAKQEVADDDDHIPISQRMKMSAAVNKSSGSKKT 360
                        D+S PL  N  ++  K++ +D DDH+PI+ RMK  ++ NKSS +K +
Sbjct: 301 HISATQDAKGKNLDASKPLRANQATV--KEDNSDGDDHVPIASRMKSDSSNNKSSSAKPS 360

Query: 361 VSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQTHG 420
            SK+ +SS +   +K  K VK SKY KS+K LP SGDGQKKW TL HNGVIFPPPY+ HG
Sbjct: 361 SSKMIASSSRTIAQKPNKWVKDSKYSKSSKSLP-SGDGQKKWKTLQHNGVIFPPPYKRHG 420

Query: 421 VKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD 480
           VKIL +GKPVDLTPEQEEVATMFAVM++T+Y +KPKF+ENFWNDWRKLLGKNH+I++LDD
Sbjct: 421 VKILFQGKPVDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDD 480

Query: 481 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPG 540
           CDFTPIY+WY  EKE KKQMT EEK+ +KEEKLKQEEKYMWA+LDGV+E++GNFRVEPPG
Sbjct: 481 CDFTPIYEWYMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRERIGNFRVEPPG 540

Query: 541 LFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWND 600
           LFRGRGEHPKMGKLKKRIRP DITINIGK+APIPECPIPG+RWKEV+HDNTVTWLAFW+D
Sbjct: 541 LFRGRGEHPKMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSD 600

Query: 601 PINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAV 660
           PINPKEFKYVFLAASSSLKGQSDKEKYEKAR L ++I +IR +YTKDF +KD T+RQIAV
Sbjct: 601 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAV 660

Query: 661 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 720
           ATYLIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEV
Sbjct: 661 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEV 720

Query: 721 ELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 780
           E  V+KAI QFQ+GKS +DDLFD+LDTSKLN HLK+LM GLTAKVFRTYNASITLD ML+
Sbjct: 721 EPLVYKAIGQFQAGKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLS 780

Query: 781 RGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKID 840
           + T+DG+V EK+V YQ ANKEVAIICNHQRTVSKSHGAQ+ +L  KI ELR+ +KEL ID
Sbjct: 781 KETRDGDVPEKVVVYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNID 840

Query: 841 LDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSK 888
           LDRAKKG+ PL GSDGK+KRNLTPEALEKKI QT  KIEK+ERDM+TKED+KTVALGTSK
Sbjct: 841 LDRAKKGRTPLMGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSK 900

BLAST of Cp4.1LG14g07500 vs. ExPASy Swiss-Prot
Match: P30181 (DNA topoisomerase 1 alpha OS=Arabidopsis thaliana OX=3702 GN=TOP1A PE=1 SV=1)

HSP 1 Score: 1010.4 bits (2611), Expect = 1.3e-293
Identity = 559/938 (59.59%), Postives = 674/938 (71.86%), Query Frame = 0

Query: 1   MAVETFGKAPMFDD---DDEDNMPIVFKRSS--ASRQNQLNSEVKHSQSQRLNQLSGRPG 60
           M  ET  K P+ D+   D +D+ P+ FKR++  AS  NQ  S  + S++    ++S    
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPM-R 60

Query: 61  SDACSANGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDS 120
           S   S NG         T   KTS ++  + S  +  S  + PL   + +T+K    +  
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQL-- 120

Query: 121 RPKQLANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKS- 180
                       + D      ++ DSEDD+PLS  L GN    S   + + Q  K     
Sbjct: 121 ------------QKDQSECKIEHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180

Query: 181 ----------SEDSDDEVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDK 240
                      ++SDDE P+SS F+ K  SG+ GG Q   DEKKPL  K+HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240

Query: 241 LQKPAVVSSKRPLPNADSHSSSQISSKKPKLSDSSTPLSTNTV--------------SLK 300
           +    V+  KRPL    S   S +   K   S +S  +  ++V               +K
Sbjct: 241 VPNGKVL-GKRPLEKNSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPKMK 300

Query: 301 AKQEVA-------DDDDHIPISQRMKMSAAVNKSSGSKKTVSKVASSS------------ 360
           AKQ          DDDD +PIS+R K  ++ + +S +K    K+ S+S            
Sbjct: 301 AKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNVVS 360

Query: 361 --IKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQTHGVKILHK 420
              +   K +KK  K SKY  S+K  PSSGDGQKKWTTLVHNGVIFPPPY+ HG+KIL+K
Sbjct: 361 PRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKILYK 420

Query: 421 GKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDDCDFTPI 480
           GKPVDLT EQEEVATMFAVM++TDY  KP+F+ENFWNDWR+LLGK HVIQ LDDCDFTPI
Sbjct: 421 GKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFTPI 480

Query: 481 YDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLFRGRG 540
           Y+W+  EKEKKKQM+TEEKKALKEEK+KQEEKYMWA++DGVKEK+GNFRVEPPGLFRGRG
Sbjct: 481 YEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLFRGRG 540

Query: 541 EHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWNDPINPKE 600
           EHPKMGKLKKRI P +IT+NIGK APIPECPI G+RWKEV+HDNTVTWLAFW DPINPKE
Sbjct: 541 EHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINPKE 600

Query: 601 FKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAVATYLID 660
           FKYVFL A SSLKG SDKEKYEKAR L D+I NIR +YTK+FT+KD   RQIAVATYLID
Sbjct: 601 FKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYLID 660

Query: 661 KLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFK 720
           KLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE  V+K
Sbjct: 661 KLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYK 720

Query: 721 AITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDG 780
           AI QFQ+GKS +DDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TKDG
Sbjct: 721 AIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDG 780

Query: 781 EVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKIDLDRAKK 840
           +V++KIV YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL++++KELK +LDRAKK
Sbjct: 781 DVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKK 840

Query: 841 GKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDP 888
           GKPPL+GSDGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLDP
Sbjct: 841 GKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDP 900

BLAST of Cp4.1LG14g07500 vs. ExPASy Swiss-Prot
Match: P93119 (DNA topoisomerase 1 OS=Daucus carota OX=4039 GN=TOP1 PE=2 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 4.8e-240
Identity = 487/805 (60.50%), Postives = 573/805 (71.18%), Query Frame = 0

Query: 125 VKEENDSIHPTQDNSDSEDDKPLSIR-----LKGNSNHPSKG-----PIP-TVQKNKIKK 184
           +KEE  S     ++ DS+DDKPLS +     L GNS+H   G     P P T  K +I K
Sbjct: 22  LKEEGQS---RSNSEDSDDDKPLSHKLSSGALNGNSHHIRMGSNLSCPSPYTSPKPRIIK 81

Query: 185 SSEDSDDEVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSS 244
             E   DE+ LSS+ Q+   +G         DE KPL S           +L+ P+   +
Sbjct: 82  GPE---DEMLLSSELQLN--AGASNAESSDSDESKPLAS-----------ELEDPSSSLN 141

Query: 245 KRPLPNADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQRMKMSAA 304
           KRPL      +S    SKK KLS+ S P  TN     A+QE A  DD   IS R K S  
Sbjct: 142 KRPL--IQPKNSDPSPSKKAKLSEPSAP--TNRKLKTAEQEEAAADDDPSISNRNKKSTT 201

Query: 305 VNKSSGSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGV 364
                      SKV+    +  +  S   V  SK +K   +L    +GQ     LVHNGV
Sbjct: 202 ---------PASKVSDKKKRPDVSASVNKVDFSKSLKVPLVLVKGRNGQ----PLVHNGV 261

Query: 365 IFPPPYQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLG 424
           IFPP Y+ HGVK+L++  PVDLTPEQEEVATMFAVM +T+YM KPKF+ENF +DWRK+LG
Sbjct: 262 IFPPLYKPHGVKMLYRENPVDLTPEQEEVATMFAVMLETEYMTKPKFRENFMSDWRKILG 321

Query: 425 KNHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLK-QEEKYMWAILDGV-K 484
           + H+IQNL+DCDFTPIY+W+Q EKEKKKQM+T+EKKA+KE K + ++EKYMWA++DG+ +
Sbjct: 322 EKHIIQNLEDCDFTPIYEWHQREKEKKKQMSTDEKKAIKEGKDETRKEKYMWAVVDGMSR 381

Query: 485 EKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPS-DITINIGKDAPIPECPIPGQRWKEVR 544
           EKVGNFRV    + +      K  ++KK +    DITINIGKDAPIPE PIPG+RWKE+R
Sbjct: 382 EKVGNFRVGTTRVVQRSWRASKDPQVKKNVYSQIDITINIGKDAPIPEPPIPGERWKEIR 441

Query: 545 HDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIR-NSYTK 604
           HDNTVTWLAFWNDPINPKEFKYVFLAASSSLKG+  +EKYEK+R LKDYI+ IR  +YTK
Sbjct: 442 HDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGKVTREKYEKSRKLKDYIEGIRAAAYTK 501

Query: 605 DFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNF 664
           DF SKD  +RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV+   PN+L+F+F
Sbjct: 502 DFASKDSKKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVETKRPNILKFDF 561

Query: 665 LGKDSIKYENTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVF 724
           LGKDSI+Y+N VEVE  VF AI QF+SGK G DDLFD+LDTSKLNAHLK+LMPGLTAKVF
Sbjct: 562 LGKDSIRYQNEVEVEPRVFSAIEQFRSGKEGGDDLFDQLDTSKLNAHLKELMPGLTAKVF 621

Query: 725 RTYNASITLDDM-----LNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMT 784
           RTYNASITLD+M     L+R TK G+V+EK+V YQHANKEVAIICNHQRTVSKSH AQM 
Sbjct: 622 RTYNASITLDEMVRCKLLSRETKGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMV 681

Query: 785 RLNEKISELRDIVKELKIDLDRAKKGKPPLKGSDGKQKRNLTPE---------------- 844
           RLNEKI EL+ ++KEL+ DL R  KGKPPLK SDGK KRNL  +                
Sbjct: 682 RLNEKIEELKGLLKELQEDLTRVNKGKPPLKNSDGKPKRNLNLKRYKGKLLKLIQKLRKW 741

Query: 845 ------ALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVP 888
                 ++ KKI+QTN KIEK+ERD +TKEDLKTVALGTSKINYLDPRITVAWCKRH+VP
Sbjct: 742 SETRRLSVTKKIAQTNTKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHDVP 790

BLAST of Cp4.1LG14g07500 vs. ExPASy Swiss-Prot
Match: Q54RC3 (DNA topoisomerase 1 OS=Dictyostelium discoideum OX=44689 GN=top1 PE=3 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 7.3e-148
Identity = 343/870 (39.43%), Postives = 495/870 (56.90%), Query Frame = 0

Query: 70  KRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQLANAAVKEEN 129
           K++T  PK +P+ P+  +    K  I  P  K + ++   SDS DS     ++++   ++
Sbjct: 59  KKLTPTPKPAPVTPKPTAAVNGKKPI--PKKKPVDSS-SDSDSSDSSSDSDSDSS---DS 118

Query: 130 DSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDDEVPLSSKFQ 189
           DS   + D+S S+ D P    +    N PS G +    KN  K ++  +     + SK  
Sbjct: 119 DSDSSSSDSSSSDSDVPKKKPISKIPNKPS-GTVSKTSKNSPKTTTTTTTTTTTVKSKPA 178

Query: 190 MKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNAD----SHSS 249
            K             D     D     + S S  + +KP    SK+P            +
Sbjct: 179 PKVKPEQSSSDDSSSDSS---DDSSDDSSSDSSSEEEKPKKKPSKKPAAVKKEITVKKET 238

Query: 250 SQISSKKPKLSDSSTPLSTNTVSL-KAKQEVADDDDHIPISQRMKMSAAVNKSSGSKKTV 309
           +  + K P   +      T T +  K K+E +DDD                     +KT 
Sbjct: 239 TTTTKKTPVKKEVEVKKETKTTTTKKVKKEESDDDGE------------------DEKTT 298

Query: 310 SKVASSSIKKTLKKSKK-PVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQTHG 369
           +K  +++ K+ LK+ ++   ++ + V          D   KWTTL+HNG +FPP Y  HG
Sbjct: 299 TKKKTTTRKRKLKEEEEGEEEEEEGVNKWWDKEDDEDDGTKWTTLIHNGPVFPPAYVPHG 358

Query: 370 VKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLL--GKNHVIQNL 429
           +K ++ GK V LTP+QEEVAT +A   +TD+++K  FK+NF+ +++ LL   +  +++ +
Sbjct: 359 IKFMYDGKVVKLTPKQEEVATFYANYLETDHVKKQAFKDNFFKEFKGLLTSEQKEIVKKM 418

Query: 430 DDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEP 489
           D CDF+ I+   +T+KE++K  + EEK A   EK K +EK+ +A +DG KEKVGN+ +EP
Sbjct: 419 DKCDFSHIHTHLKTKKEQRKARSKEEKDAETAEKKKIQEKHGFATIDGFKEKVGNYMIEP 478

Query: 490 PGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFW 549
           PGLF GRG+HPK G LK+RI PSD+T+NIG+   IP  PIPG++WKE+ H+NTVTWLAFW
Sbjct: 479 PGLFLGRGQHPKTGMLKQRIMPSDVTLNIGEGEVIPPSPIPGEKWKEIVHNNTVTWLAFW 538

Query: 550 NDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQI 609
            + +N   FKYV+LA SS +KGQ+D++K+E AR LK+ I +IR  Y K F  +   +RQ+
Sbjct: 539 RESVN-GGFKYVWLAPSSKIKGQADRKKFEVARGLKNCIGDIRKGYQKSFNDESTEKRQL 598

Query: 610 AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTV 669
           AVA YLID LALR GNEK +D+ADTVGCC+L+VE++K    N +  +FLGKDS++Y NTV
Sbjct: 599 AVALYLIDFLALRVGNEKGEDQADTVGCCSLRVEHIKLESNNTITLDFLGKDSMRYLNTV 658

Query: 670 EVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDM 729
           ++    +K +  F  GK  +D LFD L  ++LN HLK  M GL+AKVFRTYNASITL   
Sbjct: 659 QIREDAYKVLKTFVKGKKPTDLLFDSLTVTQLNNHLKKQMTGLSAKVFRTYNASITLQKE 718

Query: 730 LNR-GTKDGE-VSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKE 789
           L++  TK+ + + EKI+ Y   N+EVAI+CNHQ+   K+    M++++EKI ELRD+   
Sbjct: 719 LDKMDTKEFQSIDEKILFYNRCNREVAILCNHQKAPPKNLAETMSKIDEKIQELRDLQ-- 778

Query: 790 LKIDLDRAKKGKPPLKGSDG-----------------KQKRNLTPEALEKKIS------- 849
              DL R K GK P    +                    K++   E  +K+IS       
Sbjct: 779 ---DLCRIKIGKTPKSQQEDIESREAERLPKLIQLAITNKKDKDKEKTDKEISFDVKAAF 838

Query: 850 -----------QTNAKIEKIERDMKT-------KEDLKTVALGTSKINYLDPRITVAWCK 888
                      +   KI K E  +K        K++LKTVALGTSKINYLDPRITVAWCK
Sbjct: 839 EKSRTLPDAKGKIKEKIAKYEEQIKKQEILKTGKDELKTVALGTSKINYLDPRITVAWCK 893

BLAST of Cp4.1LG14g07500 vs. ExPASy Swiss-Prot
Match: P07799 (DNA topoisomerase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=top1 PE=1 SV=2)

HSP 1 Score: 516.5 bits (1329), Expect = 5.8e-145
Identity = 344/860 (40.00%), Postives = 483/860 (56.16%), Query Frame = 0

Query: 112 SMDSRPKQLANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKI 171
           S DS    L+    +    S   +   SD E D         + NHP    +     NK 
Sbjct: 3   SSDSDSVSLSIRRRQRRGSSKRISMKESDEESD--------SSENHPLSESL-----NK- 62

Query: 172 KKSSEDSDDEVPL-----SSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQ 231
           K  SE  +D++P+     SSK  M ++S            KK   +KV  NG     K  
Sbjct: 63  KSKSESDEDDIPIRKRRASSKKNMSNSS-----------SKK--RAKVMGNGGLKNGK-- 122

Query: 232 KPAVVSSKRPLPNADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQ 291
           K AVV  +      D +  ++ S K  ++S ++   S N      K+E +D DD +P+  
Sbjct: 123 KTAVVKEEE-----DFNEIAKPSPKHKRVSKANG--SKNGAKSAVKKEESDTDDSVPLRA 182

Query: 292 RMKMSAAVNKSSGSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWT 351
              +S    KS      +   AS++  ++    +   +  K+  S  +     D  +KWT
Sbjct: 183 VSTVSLTPYKSE-----LPSGASTTQNRSPNDEEDEDEDYKWWTSENI-----DDTQKWT 242

Query: 352 TLVHNGVIFPPPYQ--THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENF 411
           TL HNGVIF PPY+     VK+++ G PV+L PE EEVA  +A M +TD+ + P F++NF
Sbjct: 243 TLEHNGVIFAPPYEPLPKNVKLIYDGNPVNLPPEAEEVAGFYAAMLETDHAKNPVFQDNF 302

Query: 412 WNDWRKLLGK---NHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEK 471
           + D+ K+  +   NH I+    CDFT ++  ++ ++E+KK M  E+KKA+K++K ++EEK
Sbjct: 303 FRDFLKVCDECNFNHNIKEFSKCDFTQMFHHFEQKREEKKSMPKEQKKAIKQKKDEEEEK 362

Query: 472 YMWAILDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPI 531
           Y W ILDG KEKVGNFR+EPPGLFRGRG HPK G LK+R+ P  ITINIG+  P+PE P+
Sbjct: 363 YKWCILDGRKEKVGNFRIEPPGLFRGRGSHPKTGSLKRRVYPEQITINIGEGVPVPE-PL 422

Query: 532 PGQRWKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIK 591
           PG +W EV+HDNTVTWLA W++ IN    KYVFLAA SSLKGQSD +KYEK+R LKDYI 
Sbjct: 423 PGHQWAEVKHDNTVTWLATWHENIN-NNVKYVFLAAGSSLKGQSDLKKYEKSRKLKDYID 482

Query: 592 NIRNSYTKDFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVP 651
           +IR  Y KD  S+    RQ   A YLID  ALRAGNEK +DEADTVGCC+L+ E+V   P
Sbjct: 483 DIRKGYRKDLKSELTVERQRGTAMYLIDVFALRAGNEKGEDEADTVGCCSLRYEHVTLKP 542

Query: 652 PNLLEFNFLGKDSIKYENTVEVELPVFKAITQF-QSGKSGSDDLFDKLDTSKLNAHLKDL 711
           P  + F+FLGKDSI+Y N VEV+  VFK +  F +  K   D +FD+L T+ LN +L  L
Sbjct: 543 PRTVVFDFLGKDSIRYYNEVEVDPQVFKNLKIFKRPPKKEGDLIFDRLSTNSLNKYLTSL 602

Query: 712 MPGLTAKVFRTYNASITLDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHG 771
           M GL+AKVFRTYNAS T+ + L +  K+  +++KI+ Y  AN+ VAI+CNHQR+V+K+H 
Sbjct: 603 MDGLSAKVFRTYNASYTMAEELKKMPKNLTLADKILFYNRANRTVAILCNHQRSVTKNHD 662

Query: 772 AQMTRLNEKISEL---RDIVKELKIDLD-RAKKGKPP----------------------- 831
            QM R  E+I  L   R  ++++ ++L+ +  K KP                        
Sbjct: 663 VQMERFAERIKALQYQRMRLRKMMLNLEPKLAKSKPELLAKEEGITDSWIVKHHETLYEL 722

Query: 832 ---------------------------------LKGSDGKQK--------RNLTP----- 888
                                            LK +D  +K        + + P     
Sbjct: 723 EKEKIKKKFDRENEKLAAEDPKSVLPESELEVRLKAADELKKALDAELKSKKVDPGRSSM 782

BLAST of Cp4.1LG14g07500 vs. NCBI nr
Match: XP_023553297.1 (DNA topoisomerase 1 beta-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1722 bits (4460), Expect = 0.0
Identity = 887/887 (100.00%), Postives = 887/887 (100.00%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
           MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
           ANGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQL
Sbjct: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120

Query: 121 ANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180
           ANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD
Sbjct: 121 ANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180

Query: 181 EVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
           EVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA
Sbjct: 181 EVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240

Query: 241 DSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQRMKMSAAVNKSSGS 300
           DSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQRMKMSAAVNKSSGS
Sbjct: 241 DSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQRMKMSAAVNKSSGS 300

Query: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
           KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ
Sbjct: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360

Query: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
           THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN
Sbjct: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420

Query: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
           LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE
Sbjct: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480

Query: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
           PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF
Sbjct: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540

Query: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
           WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ
Sbjct: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600

Query: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
           IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT
Sbjct: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660

Query: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
           VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD
Sbjct: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720

Query: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKEL 780
           MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKEL
Sbjct: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKEL 780

Query: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
           KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG
Sbjct: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840

Query: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887
           TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887

BLAST of Cp4.1LG14g07500 vs. NCBI nr
Match: KAG7015549.1 (DNA topoisomerase 1 beta [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1711 bits (4430), Expect = 0.0
Identity = 879/887 (99.10%), Postives = 885/887 (99.77%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
           MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
           ANGQSSNAQKRMTSLPKTSPMRPEIGSQKA+KSSIQEPLVKSLGTTLKVSDSMDSRPKQL
Sbjct: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120

Query: 121 ANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180
           ANAA+KEENDSIHPTQ+NSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD
Sbjct: 121 ANAALKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180

Query: 181 EVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
           EVPLSSKFQMKSASGVLGG+QHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA
Sbjct: 181 EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240

Query: 241 DSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQRMKMSAAVNKSSGS 300
           DSHSSSQIS KKPKLSDSSTPLSTNTVSLKAKQEV DDDDHIPISQRMKMSAAVNKSSGS
Sbjct: 241 DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300

Query: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
           KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ
Sbjct: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360

Query: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
           THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN
Sbjct: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420

Query: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
           LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE
Sbjct: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480

Query: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
           PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF
Sbjct: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540

Query: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
           WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ
Sbjct: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600

Query: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
           IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT
Sbjct: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660

Query: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
           VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD
Sbjct: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720

Query: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKEL 780
           MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+KEL
Sbjct: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780

Query: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
           KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG
Sbjct: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840

Query: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887
           TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDP+FRF
Sbjct: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPEFRF 887

BLAST of Cp4.1LG14g07500 vs. NCBI nr
Match: XP_022932254.1 (DNA topoisomerase 1 beta-like [Cucurbita moschata] >XP_022932255.1 DNA topoisomerase 1 beta-like [Cucurbita moschata] >XP_022932256.1 DNA topoisomerase 1 beta-like [Cucurbita moschata] >XP_022932257.1 DNA topoisomerase 1 beta-like [Cucurbita moschata])

HSP 1 Score: 1710 bits (4428), Expect = 0.0
Identity = 880/887 (99.21%), Postives = 884/887 (99.66%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
           MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
           ANGQSSNAQKRMTSLPKTSPMRPEIGSQKA+KSSIQEPLVKSLGTTLKVSDSMDSRPKQL
Sbjct: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120

Query: 121 ANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180
           ANAAVKEENDSIHPTQ+NSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD
Sbjct: 121 ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180

Query: 181 EVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
           EVPLSSKFQMKSASGVLGG+QHGF EKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA
Sbjct: 181 EVPLSSKFQMKSASGVLGGKQHGFHEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240

Query: 241 DSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQRMKMSAAVNKSSGS 300
           DSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEV DDDDHIPISQRMKMSAAVNKSSGS
Sbjct: 241 DSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300

Query: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
           KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ
Sbjct: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360

Query: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
           THGVKILHKGKPVDLT EQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN
Sbjct: 361 THGVKILHKGKPVDLTTEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420

Query: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
           LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE
Sbjct: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480

Query: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
           PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF
Sbjct: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540

Query: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
           WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ
Sbjct: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600

Query: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
           IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT
Sbjct: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660

Query: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
           VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD
Sbjct: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720

Query: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKEL 780
           MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+KEL
Sbjct: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780

Query: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
           KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG
Sbjct: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840

Query: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887
           TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887

BLAST of Cp4.1LG14g07500 vs. NCBI nr
Match: XP_023007686.1 (DNA topoisomerase 1 beta-like [Cucurbita maxima] >XP_023007687.1 DNA topoisomerase 1 beta-like [Cucurbita maxima] >XP_023007688.1 DNA topoisomerase 1 beta-like [Cucurbita maxima] >XP_023007689.1 DNA topoisomerase 1 beta-like [Cucurbita maxima])

HSP 1 Score: 1703 bits (4410), Expect = 0.0
Identity = 876/887 (98.76%), Postives = 881/887 (99.32%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
           MAVETFGKA MFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS
Sbjct: 1   MAVETFGKATMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
            NGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQL
Sbjct: 61  TNGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120

Query: 121 ANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180
            NAAVKEENDSIHP Q+NSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD
Sbjct: 121 VNAAVKEENDSIHPAQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180

Query: 181 EVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
           EVPLSSKF+MKSASGVLGG+QHGFDEKKPLDSKVHQNGSTS DKLQKPAVVSSKRPLPNA
Sbjct: 181 EVPLSSKFRMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSSDKLQKPAVVSSKRPLPNA 240

Query: 241 DSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQRMKMSAAVNKSSGS 300
           DSHSSSQISSKKPKLSDSSTPLST+TVSLKAKQEV DDDDHIPISQRMKMSAAVNKSSGS
Sbjct: 241 DSHSSSQISSKKPKLSDSSTPLSTHTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300

Query: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
           KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ
Sbjct: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360

Query: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
           THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN
Sbjct: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420

Query: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
           LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE
Sbjct: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480

Query: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
           PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF
Sbjct: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540

Query: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
           WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ
Sbjct: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600

Query: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
           IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT
Sbjct: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660

Query: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
           VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD
Sbjct: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720

Query: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKEL 780
           MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+KEL
Sbjct: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780

Query: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
           KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG
Sbjct: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840

Query: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887
           TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887

BLAST of Cp4.1LG14g07500 vs. NCBI nr
Match: KAG6577484.1 (DNA topoisomerase 1 alpha, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1682 bits (4355), Expect = 0.0
Identity = 867/874 (99.20%), Postives = 871/874 (99.66%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
           MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
           ANGQSSNAQKRMTSLPKTSPMRPEIGSQKA+KSSIQEPLVKSLGTTLKVSDSMDSRPKQL
Sbjct: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120

Query: 121 ANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180
           ANAAVKEENDSIHPTQ+NSDSEDDKPLSIRLKGNSNHP KGPIPTVQKNKIKKSSEDSDD
Sbjct: 121 ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPIKGPIPTVQKNKIKKSSEDSDD 180

Query: 181 EVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
           EVPLSSKFQMKSASGVLGG+QHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA
Sbjct: 181 EVPLSSKFQMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240

Query: 241 DSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQRMKMSAAVNKSSGS 300
           DSHSSSQIS KKPKLSDSSTPLSTNTVSLKAKQEV DDDDHIPISQRMKMSAAVNKSSGS
Sbjct: 241 DSHSSSQISLKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300

Query: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
           KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ
Sbjct: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360

Query: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
           THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN
Sbjct: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420

Query: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
           LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE
Sbjct: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480

Query: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
           PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF
Sbjct: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540

Query: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
           WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ
Sbjct: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600

Query: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
           IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT
Sbjct: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660

Query: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
           VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD
Sbjct: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720

Query: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKEL 780
           MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+KEL
Sbjct: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780

Query: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
           KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG
Sbjct: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840

Query: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874
           TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK
Sbjct: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 874

BLAST of Cp4.1LG14g07500 vs. ExPASy TrEMBL
Match: A0A6J1EWI0 (DNA topoisomerase I OS=Cucurbita moschata OX=3662 GN=LOC111438616 PE=3 SV=1)

HSP 1 Score: 1710 bits (4428), Expect = 0.0
Identity = 880/887 (99.21%), Postives = 884/887 (99.66%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
           MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
           ANGQSSNAQKRMTSLPKTSPMRPEIGSQKA+KSSIQEPLVKSLGTTLKVSDSMDSRPKQL
Sbjct: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120

Query: 121 ANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180
           ANAAVKEENDSIHPTQ+NSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD
Sbjct: 121 ANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180

Query: 181 EVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
           EVPLSSKFQMKSASGVLGG+QHGF EKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA
Sbjct: 181 EVPLSSKFQMKSASGVLGGKQHGFHEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240

Query: 241 DSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQRMKMSAAVNKSSGS 300
           DSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEV DDDDHIPISQRMKMSAAVNKSSGS
Sbjct: 241 DSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300

Query: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
           KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ
Sbjct: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360

Query: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
           THGVKILHKGKPVDLT EQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN
Sbjct: 361 THGVKILHKGKPVDLTTEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420

Query: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
           LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE
Sbjct: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480

Query: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
           PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF
Sbjct: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540

Query: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
           WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ
Sbjct: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600

Query: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
           IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT
Sbjct: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660

Query: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
           VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD
Sbjct: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720

Query: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKEL 780
           MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+KEL
Sbjct: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780

Query: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
           KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG
Sbjct: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840

Query: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887
           TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887

BLAST of Cp4.1LG14g07500 vs. ExPASy TrEMBL
Match: A0A6J1KZE0 (DNA topoisomerase I OS=Cucurbita maxima OX=3661 GN=LOC111500229 PE=3 SV=1)

HSP 1 Score: 1703 bits (4410), Expect = 0.0
Identity = 876/887 (98.76%), Postives = 881/887 (99.32%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
           MAVETFGKA MFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS
Sbjct: 1   MAVETFGKATMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120
            NGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQL
Sbjct: 61  TNGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQL 120

Query: 121 ANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180
            NAAVKEENDSIHP Q+NSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD
Sbjct: 121 VNAAVKEENDSIHPAQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSDD 180

Query: 181 EVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPNA 240
           EVPLSSKF+MKSASGVLGG+QHGFDEKKPLDSKVHQNGSTS DKLQKPAVVSSKRPLPNA
Sbjct: 181 EVPLSSKFRMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSSDKLQKPAVVSSKRPLPNA 240

Query: 241 DSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQRMKMSAAVNKSSGS 300
           DSHSSSQISSKKPKLSDSSTPLST+TVSLKAKQEV DDDDHIPISQRMKMSAAVNKSSGS
Sbjct: 241 DSHSSSQISSKKPKLSDSSTPLSTHTVSLKAKQEVVDDDDHIPISQRMKMSAAVNKSSGS 300

Query: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360
           KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ
Sbjct: 301 KKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQ 360

Query: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420
           THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN
Sbjct: 361 THGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQN 420

Query: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480
           LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE
Sbjct: 421 LDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVE 480

Query: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540
           PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF
Sbjct: 481 PPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAF 540

Query: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600
           WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ
Sbjct: 541 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQ 600

Query: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660
           IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT
Sbjct: 601 IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENT 660

Query: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720
           VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD
Sbjct: 661 VEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDD 720

Query: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKEL 780
           MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+KEL
Sbjct: 721 MLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKEL 780

Query: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840
           KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG
Sbjct: 781 KIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALG 840

Query: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887
           TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 TSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887

BLAST of Cp4.1LG14g07500 vs. ExPASy TrEMBL
Match: A0A1S3BM72 (DNA topoisomerase I OS=Cucumis melo OX=3656 GN=LOC103491068 PE=3 SV=1)

HSP 1 Score: 1576 bits (4080), Expect = 0.0
Identity = 814/889 (91.56%), Postives = 846/889 (95.16%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
           MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQL+SE+KHSQ+QRLN+L GR GSD CS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS 60

Query: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSS-IQEPLVKSLGTTLKVSDSMDSRPKQ 120
            NGQSSN QKRMTSLPKTSPMR EIGSQKA KSS IQEPL KSLG +LK SDSMDSRPK 
Sbjct: 61  TNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPKN 120

Query: 121 LANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180
           L NAAVKEEN SI+P+Q+NSDSEDDKPLSIRLKGNSN P+KG + T+ KNK+KKSS DSD
Sbjct: 121 LVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDSD 180

Query: 181 DEVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240
           DEVPLSSKFQMK ASG +G +Q GFDEKKPL  KVHQNGSTSRDKLQKPAV+SSKRPLPN
Sbjct: 181 DEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240

Query: 241 ADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQRMKMS-AAVNKSS 300
            DSHS SQ S+KKPKLSDSS+PLS+  VSLKAKQEV DDDD IPISQR+K + A VNKSS
Sbjct: 241 EDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKSS 300

Query: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360
            SK TVSKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 SSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVK+L+KGKPVDLTP+QEEVATMFAVMKDTDYM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLK+RIRPSDITINIGK APIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540

Query: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600
           AFWNDPIN KEFKYVFLAASSSLKGQSDKEKYEKAR+LKDYIKNIRN+YTKDFTSKDPTR
Sbjct: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+YE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSG+DDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVK 780
           DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+K
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780

Query: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
           ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887
           LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889

BLAST of Cp4.1LG14g07500 vs. ExPASy TrEMBL
Match: A0A0A0L2W2 (DNA topoisomerase I OS=Cucumis sativus OX=3659 GN=Csa_3G063680 PE=3 SV=1)

HSP 1 Score: 1567 bits (4057), Expect = 0.0
Identity = 809/889 (91.00%), Postives = 843/889 (94.83%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
           MAVETFGKAPMFDDDDEDNMPIVFKR+SASRQNQL+SE+KHSQ+QRL++L GR GSD CS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRTSASRQNQLDSEIKHSQAQRLSKLPGRQGSDLCS 60

Query: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSS-IQEPLVKSLGTTLKVSDSMDSRPKQ 120
            NGQSSN QKRMTSLPKTSPMR EIGSQKAS+SS I EPL KSLGT+LK S SMDSRPK 
Sbjct: 61  TNGQSSNNQKRMTSLPKTSPMRSEIGSQKASRSSPIPEPLTKSLGTSLKASHSMDSRPKS 120

Query: 121 LANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180
           L NA VKEEN SI+P+Q+NSDSEDDKPLSIRLKGN N P+KG + TV KNK+KKS  DSD
Sbjct: 121 LVNAVVKEENVSINPSQENSDSEDDKPLSIRLKGNPNQPNKGLVSTVPKNKVKKSLADSD 180

Query: 181 DEVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240
           DEVPLSSKFQMK ASGVLG +QHGFDEKKPL  KVHQNGSTSRDKLQKPAV+SSKRPLPN
Sbjct: 181 DEVPLSSKFQMKHASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240

Query: 241 ADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQRMK-MSAAVNKSS 300
            DSHS SQ  +KKPKL+DSS+PL++  VSLKAKQEV DDDD IPISQRMK +SA VNKSS
Sbjct: 241 EDSHSPSQFPTKKPKLTDSSSPLNSKLVSLKAKQEVDDDDDDIPISQRMKKVSATVNKSS 300

Query: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360
            SK T+SKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 SSKPTISKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVK+L+KGKPVDLTP QEEVATMFAVMKDTDYM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKMLYKGKPVDLTPPQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKA+KEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKAVKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLK+RI PSDITINIGK APIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKRRICPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540

Query: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600
           AFWNDPIN KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRN+YTKDFTSKDPTR
Sbjct: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+YE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSG+DDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVK 780
           DDMLNRGTK+GEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+K
Sbjct: 721 DDMLNRGTKNGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780

Query: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
           ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887
           LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889

BLAST of Cp4.1LG14g07500 vs. ExPASy TrEMBL
Match: A0A5A7VHI9 (DNA topoisomerase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G002170 PE=3 SV=1)

HSP 1 Score: 1565 bits (4051), Expect = 0.0
Identity = 814/907 (89.75%), Postives = 846/907 (93.27%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60
           MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQL+SE+KHSQ+QRLN+L GR GSD CS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS 60

Query: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSS-IQEPLVKSLGTTLKVSDSMDSRPKQ 120
            NGQSSN QKRMTSLPKTSPMR EIGSQKA KSS IQEPL KSLG +LK SDSMDSRPK 
Sbjct: 61  TNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPKN 120

Query: 121 LANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180
           L NAAVKEEN SI+P+Q+NSDSEDDKPLSIRLKGNSN P+KG + T+ KNK+KKSS DSD
Sbjct: 121 LVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDSD 180

Query: 181 DEVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240
           DEVPLSSKFQMK ASG +G +Q GFDEKKPL  KVHQNGSTSRDKLQKPAV+SSKRPLPN
Sbjct: 181 DEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240

Query: 241 ADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQRMKMS-AAVNKSS 300
            DSHS SQ S+KKPKLSDSS+PLS+  VSLKAKQEV DDDD IPISQR+K + A VNKSS
Sbjct: 241 EDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKSS 300

Query: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360
            SK TVSKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 SSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVK+L+KGKPVDLTP+QEEVATMFAVMKDTDYM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLK+RIRPSDITINIGK APIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540

Query: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600
           AFWNDPIN KEFKYVFLAASSSLKGQSDKEKYEKAR+LKDYIKNIRN+YTKDFTSKDPTR
Sbjct: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+YE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSG+DDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVK 780
           DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+K
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780

Query: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
           ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEK------------------IFNKSLLAKFAWAMD 887
           LGTSKINYLDPRITVAWCKRHEVPIEK                  IFNKSLLAKFAWAMD
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKFKNFLLIFVIHNHNLLPQIFNKSLLAKFAWAMD 900

BLAST of Cp4.1LG14g07500 vs. TAIR 10
Match: AT5G55310.1 (DNA topoisomerase 1 beta )

HSP 1 Score: 1026.9 bits (2654), Expect = 9.4e-300
Identity = 569/944 (60.28%), Postives = 689/944 (72.99%), Query Frame = 0

Query: 1   MAVETFGKAPM------FDDDDEDNMPIVFKRSS-----ASRQNQLNSEVKHSQSQRLNQ 60
           MA E F K  +      ++D+DED++P+VFKR+S      +R + +N+ +++S       
Sbjct: 1   MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKRNSNTAATTNRPSPINNAMRNSAI----- 60

Query: 61  LSGRPGSDACSANGQS--SNAQKRMTSLPKTSPMRPEI-GSQKASKSSIQEPLVKSLGTT 120
                GS   S   +S  ++  +  +S  ++S M+P +  S    +S+++ PL       
Sbjct: 61  -----GSTKSSPPMRSPLTSPNRSASSSTRSSMMKPALPSSSSVQRSTLKSPLRDDRSVV 120

Query: 121 LKVSDSMDSRPKQLANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPT- 180
            K  +     P                   D+ DSEDDKPLS RLK +S   +K P  + 
Sbjct: 121 AKERNGFGKAPSV--------------SKSDDEDSEDDKPLSARLKLDSKEVTKQPSSSG 180

Query: 181 -------VQKNKIKKSSEDS-------DDEVPLSSKFQMKSASGVLGGRQHGFDEKKPLD 240
                  VQK+ ++             D+  P+SS  Q K++ G    +    ++K+PL 
Sbjct: 181 RGSTQQAVQKSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLV 240

Query: 241 SKVHQNG---STSRDKLQKPAVVSSKRPLPNADSHSSSQISSKKPKLS------------ 300
           + + +NG    T     Q PA    KRPL      SS+Q S K+PKLS            
Sbjct: 241 NNIDRNGLKPKTEGHSSQAPA----KRPLEKGS--SSNQSSVKRPKLSEPARPVKVEQGS 300

Query: 301 -------------DSSTPLSTNTVSLKAKQEVADDDDHIPISQRMKMSAAVNKSSGSKKT 360
                        D+S PL  N  ++  K++ +D DDH+PI+ RMK  ++ NKSS +K +
Sbjct: 301 HISATQDAKGKNLDASKPLRANQATV--KEDNSDGDDHVPIASRMKSDSSNNKSSSAKPS 360

Query: 361 VSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQTHG 420
            SK+ +SS +   +K  K VK SKY KS+K LP SGDGQKKW TL HNGVIFPPPY+ HG
Sbjct: 361 SSKMIASSSRTIAQKPNKWVKDSKYSKSSKSLP-SGDGQKKWKTLQHNGVIFPPPYKRHG 420

Query: 421 VKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDD 480
           VKIL +GKPVDLTPEQEEVATMFAVM++T+Y +KPKF+ENFWNDWRKLLGKNH+I++LDD
Sbjct: 421 VKILFQGKPVDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDD 480

Query: 481 CDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPG 540
           CDFTPIY+WY  EKE KKQMT EEK+ +KEEKLKQEEKYMWA+LDGV+E++GNFRVEPPG
Sbjct: 481 CDFTPIYEWYMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRERIGNFRVEPPG 540

Query: 541 LFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWND 600
           LFRGRGEHPKMGKLKKRIRP DITINIGK+APIPECPIPG+RWKEV+HDNTVTWLAFW+D
Sbjct: 541 LFRGRGEHPKMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSD 600

Query: 601 PINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAV 660
           PINPKEFKYVFLAASSSLKGQSDKEKYEKAR L ++I +IR +YTKDF +KD T+RQIAV
Sbjct: 601 PINPKEFKYVFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAV 660

Query: 661 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEV 720
           ATYLIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEV
Sbjct: 661 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEV 720

Query: 721 ELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLN 780
           E  V+KAI QFQ+GKS +DDLFD+LDTSKLN HLK+LM GLTAKVFRTYNASITLD ML+
Sbjct: 721 EPLVYKAIGQFQAGKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLS 780

Query: 781 RGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKID 840
           + T+DG+V EK+V YQ ANKEVAIICNHQRTVSKSHGAQ+ +L  KI ELR+ +KEL ID
Sbjct: 781 KETRDGDVPEKVVVYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNID 840

Query: 841 LDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSK 888
           LDRAKKG+ PL GSDGK+KRNLTPEALEKKI QT  KIEK+ERDM+TKED+KTVALGTSK
Sbjct: 841 LDRAKKGRTPLMGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSK 900

BLAST of Cp4.1LG14g07500 vs. TAIR 10
Match: AT5G55300.1 (DNA topoisomerase I alpha )

HSP 1 Score: 1010.4 bits (2611), Expect = 9.1e-295
Identity = 559/938 (59.59%), Postives = 674/938 (71.86%), Query Frame = 0

Query: 1   MAVETFGKAPMFDD---DDEDNMPIVFKRSS--ASRQNQLNSEVKHSQSQRLNQLSGRPG 60
           M  ET  K P+ D+   D +D+ P+ FKR++  AS  NQ  S  + S++    ++S    
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPM-R 60

Query: 61  SDACSANGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDS 120
           S   S NG         T   KTS ++  + S  +  S  + PL   + +T+K    +  
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQL-- 120

Query: 121 RPKQLANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKS- 180
                       + D      ++ DSEDD+PLS  L GN    S   + + Q  K     
Sbjct: 121 ------------QKDQSECKIEHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180

Query: 181 ----------SEDSDDEVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDK 240
                      ++SDDE P+SS F+ K  SG+ GG Q   DEKKPL  K+HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240

Query: 241 LQKPAVVSSKRPLPNADSHSSSQISSKKPKLSDSSTPLSTNTV--------------SLK 300
           +    V+  KRPL    S   S +   K   S +S  +  ++V               +K
Sbjct: 241 VPNGKVL-GKRPLEKNSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPKMK 300

Query: 301 AKQEVA-------DDDDHIPISQRMKMSAAVNKSSGSKKTVSKVASSS------------ 360
           AKQ          DDDD +PIS+R K  ++ + +S +K    K+ S+S            
Sbjct: 301 AKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNVVS 360

Query: 361 --IKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQTHGVKILHK 420
              +   K +KK  K SKY  S+K  PSSGDGQKKWTTLVHNGVIFPPPY+ HG+KIL+K
Sbjct: 361 PRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKILYK 420

Query: 421 GKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDDCDFTPI 480
           GKPVDLT EQEEVATMFAVM++TDY  KP+F+ENFWNDWR+LLGK HVIQ LDDCDFTPI
Sbjct: 421 GKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFTPI 480

Query: 481 YDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLFRGRG 540
           Y+W+  EKEKKKQM+TEEKKALKEEK+KQEEKYMWA++DGVKEK+GNFRVEPPGLFRGRG
Sbjct: 481 YEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLFRGRG 540

Query: 541 EHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWNDPINPKE 600
           EHPKMGKLKKRI P +IT+NIGK APIPECPI G+RWKEV+HDNTVTWLAFW DPINPKE
Sbjct: 541 EHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINPKE 600

Query: 601 FKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAVATYLID 660
           FKYVFL A SSLKG SDKEKYEKAR L D+I NIR +YTK+FT+KD   RQIAVATYLID
Sbjct: 601 FKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYLID 660

Query: 661 KLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFK 720
           KLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE  V+K
Sbjct: 661 KLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYK 720

Query: 721 AITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDG 780
           AI QFQ+GKS +DDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TKDG
Sbjct: 721 AIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDG 780

Query: 781 EVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKIDLDRAKK 840
           +V++KIV YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL++++KELK +LDRAKK
Sbjct: 781 DVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKK 840

Query: 841 GKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDP 888
           GKPPL+GSDGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLDP
Sbjct: 841 GKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDP 900

BLAST of Cp4.1LG14g07500 vs. TAIR 10
Match: AT5G55300.2 (DNA topoisomerase I alpha )

HSP 1 Score: 1005.4 bits (2598), Expect = 2.9e-293
Identity = 557/935 (59.57%), Postives = 671/935 (71.76%), Query Frame = 0

Query: 1   MAVETFGKAPMFDD---DDEDNMPIVFKRSS--ASRQNQLNSEVKHSQSQRLNQLSGRPG 60
           M  ET  K P+ D+   D +D+ P+ FKR++  AS  NQ  S  + S++    ++S    
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPM-R 60

Query: 61  SDACSANGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDS 120
           S   S NG         T   KTS ++  + S  +  S  + PL   + +T+K    +  
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQL-- 120

Query: 121 RPKQLANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKS- 180
                       + D      ++ DSEDD+PLS  L GN    S   + + Q  K     
Sbjct: 121 ------------QKDQSECKIEHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180

Query: 181 ----------SEDSDDEVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDK 240
                      ++SDDE P+SS F+ K  SG+ GG Q   DEKKPL  K+HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240

Query: 241 LQKPAVVSSKRPLPNADSHSSSQISSKKPKLSDSSTPLSTNTV--------------SLK 300
           +    V+  KRPL    S   S +   K   S +S  +  ++V               +K
Sbjct: 241 VPNGKVL-GKRPLEKNSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPKMK 300

Query: 301 AKQEVA-------DDDDHIPISQRMKMSAAVNKSSGSKKTVSKVASSS------------ 360
           AKQ          DDDD +PIS+R K  ++ + +S +K    K+ S+S            
Sbjct: 301 AKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNVVS 360

Query: 361 --IKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPPYQTHGVKILHK 420
              +   K +KK  K SKY  S+K  PSSGDGQKKWTTLVHNGVIFPPPY+ HG+KIL+K
Sbjct: 361 PRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKILYK 420

Query: 421 GKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVIQNLDDCDFTPI 480
           GKPVDLT EQEEVATMFAVM++TDY  KP+F+ENFWNDWR+LLGK HVIQ LDDCDFTPI
Sbjct: 421 GKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFTPI 480

Query: 481 YDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLFRGRG 540
           Y+W+  EKEKKKQM+TEEKKALKEEK+KQEEKYMWA++DGVKEK+GNFRVEPPGLFRGRG
Sbjct: 481 YEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLFRGRG 540

Query: 541 EHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWLAFWNDPINPKE 600
           EHPKMGKLKKRI P +IT+NIGK APIPECPI G+RWKEV+HDNTVTWLAFW DPINPKE
Sbjct: 541 EHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINPKE 600

Query: 601 FKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAVATYLID 660
           FKYVFL A SSLKG SDKEKYEKAR L D+I NIR +YTK+FT+KD   RQIAVATYLID
Sbjct: 601 FKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYLID 660

Query: 661 KLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFK 720
           KLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE  V+K
Sbjct: 661 KLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYK 720

Query: 721 AITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDG 780
           AI QFQ+GKS +DDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TKDG
Sbjct: 721 AIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDG 780

Query: 781 EVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKIDLDRAKK 840
           +V++KIV YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL++++KELK +LDRAKK
Sbjct: 781 DVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKK 840

Query: 841 GKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDP 885
           GKPPL+GSDGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLDP
Sbjct: 841 GKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDP 900

BLAST of Cp4.1LG14g07500 vs. TAIR 10
Match: AT5G55300.3 (DNA topoisomerase I alpha )

HSP 1 Score: 425.2 bits (1092), Expect = 1.3e-118
Identity = 219/332 (65.96%), Postives = 265/332 (79.82%), Query Frame = 0

Query: 556 AASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTRRQIAVATYLIDKLALRA 615
           A  SSLK           +M +D +K   +   +   S     +Q++      D      
Sbjct: 234 ADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPKMKAKQLSTREDGTDD----D 293

Query: 616 GNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQ 675
            ++ DDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE  V+KAI QFQ
Sbjct: 294 DDDDDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQ 353

Query: 676 SGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGEVSEKI 735
           +GKS +DDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TKDG+V++KI
Sbjct: 354 AGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDGDVTQKI 413

Query: 736 VTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKIDLDRAKKGKPPLK 795
           V YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL++++KELK +LDRAKKGKPPL+
Sbjct: 414 VVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLE 473

Query: 796 GSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAW 855
           GSDGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLDPRITVAW
Sbjct: 474 GSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDPRITVAW 533

Query: 856 CKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 888
           CKRHEVPIEKIF KSLL KFAWAMDV+P++RF
Sbjct: 534 CKRHEVPIEKIFTKSLLEKFAWAMDVEPEYRF 561

HSP 2 Score: 86.3 bits (212), Expect = 1.4e-16
Identity = 97/316 (30.70%), Postives = 146/316 (46.20%), Query Frame = 0

Query: 1   MAVETFGKAPMFDD---DDEDNMPIVFKRSS--ASRQNQLNSEVKHSQSQRLNQLSGRPG 60
           M  ET  K P+ D+   D +D+ P+ FKR++  AS  NQ  S  + S++    ++S    
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPM-R 60

Query: 61  SDACSANGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDS 120
           S   S NG         T   KTS ++  + S  +  S  + PL   + +T+K    +  
Sbjct: 61  SPVTSPNG--------TTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQL-- 120

Query: 121 RPKQLANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKS- 180
                       + D      ++ DSEDD+PLS  L GN    S   + + Q  K     
Sbjct: 121 ------------QKDQSECKIEHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180

Query: 181 ----------SEDSDDEVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDK 240
                      ++SDDE P+SS F+ K  SG+ GG Q   DEKKPL  K+HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240

Query: 241 LQKPAVVSSKRPLPNADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPI 300
           +    V+  KRPL    + S+ Q S KK K+S S T +     S+K +    DD   + +
Sbjct: 241 VPNGKVL-GKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKE---IDDKGRVLV 286

BLAST of Cp4.1LG14g07500 vs. TAIR 10
Match: AT4G26701.1 (DNA binding;DNA topoisomerase type Is )

HSP 1 Score: 129.0 bits (323), Expect = 1.9e-29
Identity = 60/68 (88.24%), Postives = 64/68 (94.12%), Query Frame = 0

Query: 820 KIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAM 879
           KIEK+ERDM+TKEDLKTVALGTSKINY+DPRITVAWCKRHE PIEKIFNKSLL KFAWAM
Sbjct: 2   KIEKMERDMQTKEDLKTVALGTSKINYMDPRITVAWCKRHEAPIEKIFNKSLLEKFAWAM 61

Query: 880 DVDPDFRF 888
           DV+P F F
Sbjct: 62  DVEPHFTF 69