Cp4.1LG14g05720 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG14g05720
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionPhosphopyruvate hydratase
LocationCp4.1LG14: 803290 .. 808683 (+)
RNA-Seq ExpressionCp4.1LG14g05720
SyntenyCp4.1LG14g05720
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATTCGACGGTCCCAGTATGCTTCACGACAGAATTTGTCACCACGCACGACTATTTATATTCTTTCATTGCGCGCCATTGGCGTAATTTTCTCGGGAAAATTGTGGAGAAAGACAAGTAAGACGAAGGGGGGAGTAAGTTTGAACTCAGTATGGCAGCGATCGAGATCGTCAAAGCCCGTCAAATTTTTGATAGTCGTGGAAATCCTACTGTCGAAGTGAGTTTTAACTTCCTCTGAATCAATCCGTCGAAAATGTCTCACCGGAATTGTTACCTGTTATTCTACAATCGTCTTGTTGGTTTTTAGCCTAACATTGGTTGCATTGTGTTTTTTCTTTCTGAGAAATGTGAGAATAGAAAGAGATTGTGAACCTATTGCGGTTATCGATTACTGTAACTGACCGTGTACATATAGATTCCTTGGCGTGAGTGTTGTACAGAGTTTGGGAAAATTTGAATGTAGAAGTTAGTATGATCGCTATTGTAAACGATTGTTTGGTGTTTTTTTGTTTTGTTGATTTGAATTTGGTTTCAGGTTGACATAACTCTTACTGATGGAACGCTGGCCAGAGCTGCTGTTCCGAGCGGTGCTTCTACCGGTAAATCGATAGTGTTTGTTTATCTAACTGATTGAAAGTAATTATTCACGGTTTGACCTTTTCTGGATATAGATGACGAATGTATGGTAGGTGATGATTCAATCGGTAGGTCGTTAGTGCTTGTTTATCGAAGAAAAGTAGATGCTACGAAATTTATTAAAAATGTACTTACCATACGTGAAAATTTTGAGAGGCGACCATTACATTTGTCTTGGAAACTTATTAAACATCTTCTGAGTATGCTTTAAAAAAACTGAAATCACTGCGGAAAGCTTTAAGTATAGACATCTTTGAGGTCATGAGTTCAACTTCAAATAACTGTAACCATATACCTTCAAGTTTCCCTTCAAAGAATTTTGCAAGGTCTAACAGTGATCCAAATGAGATTAGTAGAAAATTTGGTCAGAACATTCAATATGACTAATAAACAAAATGAAGGAAAACACAAATCCTTAAGACAGGGTATCATTTCATCTCTGCACGTATGTATTGTATATTTATCGTCACCTATAGTTGTTAGATTTCATGATTTTCCCTTGTGCCCATTGATATAGTTGTATGAATGTCCTGTGCAAAAAAATGGTCTAAACATTTCGTTTATACTTCATAGTTTTAACTATTCATATTTTATTGACTGAAGGTATCTATGAAGCTCTTGAGCTGAGAGATGGTGGGTCTGACTACCTTGGAAAGGGAGTACTTGAGGTTTGTATGGTAAAATCGGATATTTAAGTAAATTTCCCAGTGTTTGTTGTTAGGAACTTAGGAAATAACATTTTCCAAAGAAACATGTACTTTTTTTTTCTCTTGCATGTAAGACATTTTGCTATGGTTGGAGGGAGAAAAACTATGCAGCAACTTTTTTCCTCTTGCATACAAGACTATTTGTGTTTGCGTGTTTTAAAGTTGCCTAATGTGACTGCATGACTTGATTTAGGCTGTGGAAAATGTAAATTCAATCATTGGACCTGCTTTAATTGGCAAGGTAAAAGCAATAATTTCATTAGCATTTTTTTTCTACTGTCAGTGAATATGAGTGAAGTTTATATAGTTCATTCAATTTTCTGTGGCATTTCCAAGGACCCAAGAGAGCAGACAAAACTAGACAATTTTATGGTGCGGCAACTTGATGGGACCGTCAATGAATGGGGTTGGTGCAAACAGAAGGTATGTTGAACCTTTTTTTGTTCCACCGAGGTTGATGTCTCCAATGTTATGCTAACTTAGGCTAACGGGATTTTCCCTCTTCATTCTCACAGCTTGGAGCAAATGCTATACTGGCAGTCTCCCTTGCTATTTGTAAAGCTGGAGCTGCAGTGAACAAGATACCCCTCTATAAGGTATTTGTAGTCTCACGTGGTTTTAGAAGAGGGAATTCGTTTTGATTTTTTGTTTTTTTTTTTTCCTAGTCTTATATCGTCACTTGTCTTTCTTGGAACTTCTAGCACATTGCCAAGCTTGCTGGGAATAAGAAGTTGGTTCTTCCGGTCCCTGCATTCAACGTCATTAATGGAGGTTCTCATGCAGGCAATAAGCTAGCCATGCAGGTAATATGGTTTTGATATTTTTTCCCTTTTTGATAGATTAGGTGCTTTACTTTGGAACTTTGTGTTATTTTTCTGGTATGTAGGAATTTATGATTCTCCCTGTTGGAGCATCTTCTTTTAAGGAAGCTATGAAAATGGGTGTGGAAGTCTACCATCACCTCAAGGTTGTTTGCAATCCCCTTGTACCCCAGTTTTCTAAATTTATTTATACAAAGCGGTTGTTGTATCAATATATTTCTGTCTGTCTGCTTTTCAGGCTGTAATAAAAAAGAAATATGGACAAGATGCTACTAATGTTGGTGATGAAGGCGGCTTTGCCCCTAATATTCAGGTCTTAGAAACATTCGCCTACAGCATATTGTTATTTTCAAAGTTGGAGTTTTCAATGATTATTTGGCTATTGATGTTTTTCTCTTCTTTCTTTAGTCCATTCCTCTTTTCCTTTATGGTTGGAGGGAAAGGTTTGTGATCGGACAGAAACGCATATTCTAGTCAGATAGTTTCCTTCACCATTATTGTCATTTATGACTTATGCTTGGTGTTTTTTATTTCACCATGCACACTACCGAGTGGCTAATATTGATTTATATAAATTTCAGGAGAATAAGGAGGGTCTTGAACTCTTGAAGGTTGCTATAGCGAAAGCTGGATACACTGGAAAGGTGCTCTCCTTTTGTCTTTTTATTAACGTATCGAATAATATATTTCCTGATAGTACCTAGGTCCAAGTCCTTGATTATACTCTTTTGCCACTTCTAGGTTGTAATTGGAATGGATGTAGCTGCTTCAGAATTTTATGATAACAAGGCCAAGACCTATGACCTGAATTTTAAGGAAGAGGTATTTGACTGTTGGCTGGTCATGGTTCAAGTATTTTTCATGTTACACATCAGCCTGATGATAGATTTTGCTGCAGAACAATGATGGATCAGAAAAAATTTCTGGAGACAGCTTGAAGAATGTCTACAAGTCATTTGTGACAGATTATCCTATTGTGTCCATTGAAGATCCATTCGATCAGGATGATTGGGAGAATTATGCGAAGCTGACTAGTGAAATTGGCCGGCAAGTCCAGATAGTTGGAGACGACCTCTTGGTTACCAACCCAAAGGTCTTAAATGTTTTAATCATCATGTACATTCTGGTTTTCTGAATCAACATTCTGACATTATCTTGCATCTTGTAACAATTGCAGCGTGTAGAGAAAGCTATCAAGGAGAAGGCATGCAATTCCCTTCTCTTGAAGGTAATCACTCCACCTTTTCTTCCTCATAAGTATCCATTTTCCCTCTTATGTGCTTAACTTTCTTTTGGCAGCTATCTGTTTGTTACCATACTTTAGTGCTCAAGTAATTTGCAGTATATTCATAATGTTGAGATTCTTATGATATATTTATCAGGTAAATCAAATTGGTTCAGTGACTGAAAGTATTGAAGCAGTCAAGTTGTCTAAGCACGCTGGTTGGGGTGTTATGGCAAGCCATAGAAGGTATGCTACGTTTCATTGCATAGCCATCTCCCCCTTTTTTTTTTTTTCTTTTTTTTCTTTTCTTTAATTGCATGTTATAATCTCTTTCACCCAGCCTCCATGTACTATTGATTATATTTTTCTCCTTTTTGTGCAATTTAGTGGGGAAACTGAGGATACGTTCATTGCAGACCTATCAGTTGGCCTGTCAACAGTAAGTGCTAACAATTCCCCCATGATTGAAACTCTGTAGTTTTACCTGTAAATTTGTTCGTAGAATTATTGTTACTGTATAAGCATGCCCTGGTCCCTGCATAGGCATATTGCTCACGCTGTGAGTGATGATATTTCTTATGGAATTTTAATCCAAGATGTAGCCTTTTGAAGGAGAAAGTAACATTTATGTGCATTTTTTACTTGTTTTTTTAAATTGTGCTTACTTTCATTTTGAACTCTACAAAATTCCAAAAACAAAAACAAGTTTTTAAAATTTAGTTCTTTTAGTTTTCAAAATTTGGCTTGGATTTTGAAAATATTTCTAAAAAAAAATGTACATAATGAAACAAAGAAGCTAATATGTAAAATTGATGATTATAAGTTTAAAAAACCAAATGATTATCAGAAAGATCTTATATTTAATGGTTTCATCATTATCCAGTTGGTCATATTTAAATATCATTTCTTTGTTTGAGGTGGTACCAATCAAATCTACTAACGTTTACAAAGCATTGGTTGTAAATCTTTGTTTATGTGTGTGCATATTGAGTTTTTTAAATTATCTTCAATTCCTTGACGGTCATTTGAATGTTTCCCAAACTGCAAAACAGGGTCAGATTAAGACTGGTGCACCCTGTAGATCTGAGCGCCTAGCCAAATATAACCAGGTGAATATTATTAGTTGAAATTTTGCCTCTTGCAAATGAAGATTAGGATAGTGGGCAAGACCATCTAAATTTCTTTTAAGGTTATTAGTTCTAGAAGTTGAAATTTAGTAACCATATTTTCAGAAAATCTTAAAATTAGGGTATTGTAACCTTTATTTAATTATTGTCTGCTCTTACAATAACAAAACCTACAACTAAATAAGACTTTGTTTATGATTTGACCACCGGTTGACCAGTTTTTTCAACTGTGATCTTTGCTTTTCCAACATTCATACAGCTCAAATTTGGGCGCTTGTAACACAATCAACTGCTTTCTTTTTTTTCCCTGCAGCTCCTTAGGATAGAAGAAGAGCTCGGACCAGCTGCAATTTATGCTGGATCAAAATTTCGATCACCAGTGGAGCCTTATTGAGATTTTAATCATGAGAACGCAACCACTTCACTATGTTGATGATTTTGTATGGAACATATGAAATGAACTTTTTTCTGGTAGCAGGGAGGTGATGAGGTGCTCTACGCATGTTATATTAGACTAGGTTTAATCTCGATTATCTCTTTTACTAGAAATGCTGCGATTTTCTAGCCTGCTTCTTTCTGTTTCTATTGTTTTCTTTTTCGTTACTTTTCCTGGAAAAAAATCACTTGGAGTGGAGGTTAGCAACCTGGTTCTAGCGAATTTAACCAAACTTTGGTCCCAGTCTGAGTTTTTACCCCCCAAAGATTGGACCTAAACTGAACTATGCACTCTTAACTCCTAGGTCCATCTATGGAAGATTGTCCTAGGAAGGGCCAGTTTATTTGCAGGAGCTTGCTGGTCGTTACCTTTAGATTTTAGATGCTCTAAAATTAGACAGCCAACATCTTTTAACTATGATATTTGTCAATCCAATAATTCTT

mRNA sequence

TATTCGACGGTCCCAGTATGCTTCACGACAGAATTTGTCACCACGCACGACTATTTATATTCTTTCATTGCGCGCCATTGGCGTAATTTTCTCGGGAAAATTGTGGAGAAAGACAAGTAAGACGAAGGGGGGAGTAAGTTTGAACTCAGTATGGCAGCGATCGAGATCGTCAAAGCCCGTCAAATTTTTGATAGTCGTGGAAATCCTACTGTCGAAGTTGACATAACTCTTACTGATGGAACGCTGGCCAGAGCTGCTGTTCCGAGCGGTGCTTCTACCGGTATCTATGAAGCTCTTGAGCTGAGAGATGGTGGGTCTGACTACCTTGGAAAGGGAGTACTTGAGGCTGTGGAAAATGTAAATTCAATCATTGGACCTGCTTTAATTGGCAAGGACCCAAGAGAGCAGACAAAACTAGACAATTTTATGGTGCGGCAACTTGATGGGACCGTCAATGAATGGGGTTGGTGCAAACAGAAGCTTGGAGCAAATGCTATACTGGCAGTCTCCCTTGCTATTTGTAAAGCTGGAGCTGCAGTGAACAAGATACCCCTCTATAAGCACATTGCCAAGCTTGCTGGGAATAAGAAGTTGGTTCTTCCGGTCCCTGCATTCAACGTCATTAATGGAGGTTCTCATGCAGGCAATAAGCTAGCCATGCAGGAATTTATGATTCTCCCTGTTGGAGCATCTTCTTTTAAGGAAGCTATGAAAATGGGTGTGGAAGTCTACCATCACCTCAAGGCTGTAATAAAAAAGAAATATGGACAAGATGCTACTAATGTTGGTGATGAAGGCGGCTTTGCCCCTAATATTCAGGAGAATAAGGAGGGTCTTGAACTCTTGAAGGTTGCTATAGCGAAAGCTGGATACACTGGAAAGGTTGTAATTGGAATGGATGTAGCTGCTTCAGAATTTTATGATAACAAGGCCAAGACCTATGACCTGAATTTTAAGGAAGAGAACAATGATGGATCAGAAAAAATTTCTGGAGACAGCTTGAAGAATGTCTACAAGTCATTTGTGACAGATTATCCTATTGTGTCCATTGAAGATCCATTCGATCAGGATGATTGGGAGAATTATGCGAAGCTGACTAGTGAAATTGGCCGGCAAGTCCAGATAGTTGGAGACGACCTCTTGGTTACCAACCCAAAGCGTGTAGAGAAAGCTATCAAGGAGAAGGCATGCAATTCCCTTCTCTTGAAGGTAAATCAAATTGGTTCAGTGACTGAAAGTATTGAAGCAGTCAAGTTGTCTAAGCACGCTGGTTGGGGTGTTATGGCAAGCCATAGAAGTGGGGAAACTGAGGATACGTTCATTGCAGACCTATCAGTTGGCCTGTCAACAGGTCAGATTAAGACTGGTGCACCCTGTAGATCTGAGCGCCTAGCCAAATATAACCAGCTCCTTAGGATAGAAGAAGAGCTCGGACCAGCTGCAATTTATGCTGGATCAAAATTTCGATCACCAGTGGAGCCTTATTGAGATTTTAATCATGAGAACGCAACCACTTCACTATGTTGATGATTTTGTATGGAACATATGAAATGAACTTTTTTCTGGTAGCAGGGAGGTGATGAGGTGCTCTACGCATGTTATATTAGACTAGGTTTAATCTCGATTATCTCTTTTACTAGAAATGCTGCGATTTTCTAGCCTGCTTCTTTCTGTTTCTATTGTTTTCTTTTTCGTTACTTTTCCTGGAAAAAAATCACTTGGAGTGGAGGTTAGCAACCTGGTTCTAGCGAATTTAACCAAACTTTGGTCCCAGTCTGAGTTTTTACCCCCCAAAGATTGGACCTAAACTGAACTATGCACTCTTAACTCCTAGGTCCATCTATGGAAGATTGTCCTAGGAAGGGCCAGTTTATTTGCAGGAGCTTGCTGGTCGTTACCTTTAGATTTTAGATGCTCTAAAATTAGACAGCCAACATCTTTTAACTATGATATTTGTCAATCCAATAATTCTT

Coding sequence (CDS)

ATGGCAGCGATCGAGATCGTCAAAGCCCGTCAAATTTTTGATAGTCGTGGAAATCCTACTGTCGAAGTTGACATAACTCTTACTGATGGAACGCTGGCCAGAGCTGCTGTTCCGAGCGGTGCTTCTACCGGTATCTATGAAGCTCTTGAGCTGAGAGATGGTGGGTCTGACTACCTTGGAAAGGGAGTACTTGAGGCTGTGGAAAATGTAAATTCAATCATTGGACCTGCTTTAATTGGCAAGGACCCAAGAGAGCAGACAAAACTAGACAATTTTATGGTGCGGCAACTTGATGGGACCGTCAATGAATGGGGTTGGTGCAAACAGAAGCTTGGAGCAAATGCTATACTGGCAGTCTCCCTTGCTATTTGTAAAGCTGGAGCTGCAGTGAACAAGATACCCCTCTATAAGCACATTGCCAAGCTTGCTGGGAATAAGAAGTTGGTTCTTCCGGTCCCTGCATTCAACGTCATTAATGGAGGTTCTCATGCAGGCAATAAGCTAGCCATGCAGGAATTTATGATTCTCCCTGTTGGAGCATCTTCTTTTAAGGAAGCTATGAAAATGGGTGTGGAAGTCTACCATCACCTCAAGGCTGTAATAAAAAAGAAATATGGACAAGATGCTACTAATGTTGGTGATGAAGGCGGCTTTGCCCCTAATATTCAGGAGAATAAGGAGGGTCTTGAACTCTTGAAGGTTGCTATAGCGAAAGCTGGATACACTGGAAAGGTTGTAATTGGAATGGATGTAGCTGCTTCAGAATTTTATGATAACAAGGCCAAGACCTATGACCTGAATTTTAAGGAAGAGAACAATGATGGATCAGAAAAAATTTCTGGAGACAGCTTGAAGAATGTCTACAAGTCATTTGTGACAGATTATCCTATTGTGTCCATTGAAGATCCATTCGATCAGGATGATTGGGAGAATTATGCGAAGCTGACTAGTGAAATTGGCCGGCAAGTCCAGATAGTTGGAGACGACCTCTTGGTTACCAACCCAAAGCGTGTAGAGAAAGCTATCAAGGAGAAGGCATGCAATTCCCTTCTCTTGAAGGTAAATCAAATTGGTTCAGTGACTGAAAGTATTGAAGCAGTCAAGTTGTCTAAGCACGCTGGTTGGGGTGTTATGGCAAGCCATAGAAGTGGGGAAACTGAGGATACGTTCATTGCAGACCTATCAGTTGGCCTGTCAACAGGTCAGATTAAGACTGGTGCACCCTGTAGATCTGAGCGCCTAGCCAAATATAACCAGCTCCTTAGGATAGAAGAAGAGCTCGGACCAGCTGCAATTTATGCTGGATCAAAATTTCGATCACCAGTGGAGCCTTATTGA

Protein sequence

MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLGKGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVSLAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAGYTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSIEDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSVTESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQLLRIEEELGPAAIYAGSKFRSPVEPY
Homology
BLAST of Cp4.1LG14g05720 vs. ExPASy Swiss-Prot
Match: Q42971 (Enolase OS=Oryza sativa subsp. japonica OX=39947 GN=ENO1 PE=1 SV=2)

HSP 1 Score: 783.9 bits (2023), Expect = 9.8e-226
Identity = 388/444 (87.39%), Postives = 419/444 (94.37%), Query Frame = 0

Query: 2   AAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLGK 61
           A I  VKARQIFDSRGNPTVEVD+  +DGT ARAAVPSGASTG+YEALELRDGGSDYLGK
Sbjct: 3   ATIVSVKARQIFDSRGNPTVEVDVCCSDGTFARAAVPSGASTGVYEALELRDGGSDYLGK 62

Query: 62  GVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVSL 121
           GV +AV+NVNS+I PALIGKDP  Q +LDNFMV+QLDGT NEWGWCKQKLGANAILAVSL
Sbjct: 63  GVSKAVDNVNSVIAPALIGKDPTSQAELDNFMVQQLDGTKNEWGWCKQKLGANAILAVSL 122

Query: 122 AICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGAS 181
           AICKAGA + KIPLY+HIA LAGNK+LVLPVPAFNVINGGSHAGNKLAMQEFMILP GA+
Sbjct: 123 AICKAGAIIKKIPLYQHIANLAGNKQLVLPVPAFNVINGGSHAGNKLAMQEFMILPTGAA 182

Query: 182 SFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAGY 241
           SFKEAMKMGVEVYH+LK+VIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AI KAGY
Sbjct: 183 SFKEAMKMGVEVYHNLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAGY 242

Query: 242 TGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSIE 301
           TGKVVIGMDVAASEFY++K KTYDLNFKEENNDGS+KISGDSLKNVYKSFV++YPIVSIE
Sbjct: 243 TGKVVIGMDVAASEFYNDKDKTYDLNFKEENNDGSQKISGDSLKNVYKSFVSEYPIVSIE 302

Query: 302 DPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSVT 361
           DPFDQDDWE+YAK+T+EIG QVQIVGDDLLVTNP RV KAI+EK+CN+LLLKVNQIGSVT
Sbjct: 303 DPFDQDDWEHYAKMTAEIGEQVQIVGDDLLVTNPTRVAKAIQEKSCNALLLKVNQIGSVT 362

Query: 362 ESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQLL 421
           ESIEAVK+SK AGWGVM SHRSGETEDTFIADL+VGL+TGQIKTGAPCRSERLAKYNQLL
Sbjct: 363 ESIEAVKMSKRAGWGVMTSHRSGETEDTFIADLAVGLATGQIKTGAPCRSERLAKYNQLL 422

Query: 422 RIEEELGPAAIYAGSKFRSPVEPY 446
           RIEEELG AA+YAG+KFR+PVEPY
Sbjct: 423 RIEEELGAAAVYAGAKFRAPVEPY 446

BLAST of Cp4.1LG14g05720 vs. ExPASy Swiss-Prot
Match: P42896 (Enolase OS=Ricinus communis OX=3988 PE=2 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 1.7e-225
Identity = 392/439 (89.29%), Postives = 418/439 (95.22%), Query Frame = 0

Query: 7   VKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLGKGVLEA 66
           V+ARQIFDSRGNPTVE DI L+DG LARAAVPSGASTGIYEALELRDGGSDYLGKGV +A
Sbjct: 8   VRARQIFDSRGNPTVEADIKLSDGHLARAAVPSGASTGIYEALELRDGGSDYLGKGVSKA 67

Query: 67  VENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVSLAICKA 126
           VENVNSIIGPALIGKDP EQT LDNFMV++LDGTVNEWGWCKQKLGANAILAVSLA+CKA
Sbjct: 68  VENVNSIIGPALIGKDPTEQTALDNFMVQELDGTVNEWGWCKQKLGANAILAVSLALCKA 127

Query: 127 GAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEA 186
           GA V  IPLYKHIA LAGNK LVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEA
Sbjct: 128 GAHVKGIPLYKHIANLAGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEA 187

Query: 187 MKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAGYTGKVV 246
           MKMG EVYHHLK+VIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AIAKAGYTGKVV
Sbjct: 188 MKMGAEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIAKAGYTGKVV 247

Query: 247 IGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSIEDPFDQ 306
           IGMDVAASEFY +  KTYDLNFKEENNDGS+KISG++LK++YKSF ++YPIVSIEDPFDQ
Sbjct: 248 IGMDVAASEFYGSD-KTYDLNFKEENNDGSQKISGEALKDLYKSFASEYPIVSIEDPFDQ 307

Query: 307 DDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSVTESIEA 366
           DDWE+Y+KLTSEIG +VQIVGDDLLVTNPKRVEKAI+EKACN+LLLKVNQIGSVTESIEA
Sbjct: 308 DDWEHYSKLTSEIGEKVQIVGDDLLVTNPKRVEKAIQEKACNALLLKVNQIGSVTESIEA 367

Query: 367 VKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQLLRIEEE 426
           V++SK AGWGVMASHRSGETEDTFIADLSVGL+TGQIKTGAPCRSERLAKYNQLLRIEEE
Sbjct: 368 VRMSKRAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQLLRIEEE 427

Query: 427 LGPAAIYAGSKFRSPVEPY 446
           LG  A+YAG+KFR+PVEPY
Sbjct: 428 LGAEAVYAGAKFRTPVEPY 445

BLAST of Cp4.1LG14g05720 vs. ExPASy Swiss-Prot
Match: P42895 (Enolase 2 OS=Zea mays OX=4577 GN=ENO2 PE=2 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 4.9e-225
Identity = 386/444 (86.94%), Postives = 417/444 (93.92%), Query Frame = 0

Query: 2   AAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLGK 61
           A I+ VKARQIFDSRGNPTVEVD+  +DGT ARAAVPSGASTG+YEALELRDGGS YLGK
Sbjct: 3   ATIQSVKARQIFDSRGNPTVEVDVFCSDGTFARAAVPSGASTGVYEALELRDGGSYYLGK 62

Query: 62  GVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVSL 121
           GV +AV NVNS+IGPALIGKDP  QT++DNFMV+QLDGT NEWGWCKQKLGANAILAVSL
Sbjct: 63  GVSKAVNNVNSVIGPALIGKDPTAQTEIDNFMVQQLDGTKNEWGWCKQKLGANAILAVSL 122

Query: 122 AICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGAS 181
           A+CKAGA++ +IPLY+HIA LAGNK+LVLPVPAFNVINGGSHAGNKLAMQEFMILP GA+
Sbjct: 123 AVCKAGASIKRIPLYQHIANLAGNKQLVLPVPAFNVINGGSHAGNKLAMQEFMILPTGAA 182

Query: 182 SFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAGY 241
           SFKEAMKMGVEVYHHLK+VIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AI KAGY
Sbjct: 183 SFKEAMKMGVEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAGY 242

Query: 242 TGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSIE 301
           TGKVVIGMDVAASEFY +K +TYDLNFKEENNDGS+KISGDSLKNVYKSFV++YPIVSIE
Sbjct: 243 TGKVVIGMDVAASEFYSDKDQTYDLNFKEENNDGSQKISGDSLKNVYKSFVSEYPIVSIE 302

Query: 302 DPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSVT 361
           DPFDQDDW +YAK+T EIG QVQIVGDDLLVTNP RV KAIKEK+CN+LLLKVNQIGSVT
Sbjct: 303 DPFDQDDWVHYAKMTEEIGEQVQIVGDDLLVTNPTRVAKAIKEKSCNALLLKVNQIGSVT 362

Query: 362 ESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQLL 421
           ESIEAVK+SK AGWGVM SHRSGETEDTFIADL+VGLSTGQIKTGAPCRSERLAKYNQLL
Sbjct: 363 ESIEAVKMSKRAGWGVMTSHRSGETEDTFIADLAVGLSTGQIKTGAPCRSERLAKYNQLL 422

Query: 422 RIEEELGPAAIYAGSKFRSPVEPY 446
           RIEEELG  A+YAG+KFR+PVEPY
Sbjct: 423 RIEEELGAIAVYAGAKFRAPVEPY 446

BLAST of Cp4.1LG14g05720 vs. ExPASy Swiss-Prot
Match: Q9LEI9 (Enolase 2 OS=Hevea brasiliensis OX=3981 GN=ENO2 PE=1 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 9.2e-224
Identity = 389/439 (88.61%), Postives = 414/439 (94.31%), Query Frame = 0

Query: 7   VKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLGKGVLEA 66
           V+ARQIFDSRGNPTVE D+ L+DG LARAAVP GASTGIYEALELRDGGSDYLGKGV +A
Sbjct: 8   VRARQIFDSRGNPTVEADVKLSDGYLARAAVPRGASTGIYEALELRDGGSDYLGKGVSKA 67

Query: 67  VENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVSLAICKA 126
           VENVN IIGPAL+GKDP +Q  +DNFMV+QLDGTVNEWGWCKQKLGANAILAVSLA+CKA
Sbjct: 68  VENVNIIIGPALVGKDPTDQVGIDNFMVQQLDGTVNEWGWCKQKLGANAILAVSLAVCKA 127

Query: 127 GAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEA 186
           GA V  IPLYKH+A LAGNK LVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEA
Sbjct: 128 GAHVKGIPLYKHVANLAGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEA 187

Query: 187 MKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAGYTGKVV 246
           MKMG EVYHHLK+VIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AIAKAGYTGKVV
Sbjct: 188 MKMGAEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIAKAGYTGKVV 247

Query: 247 IGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSIEDPFDQ 306
           IGMDVAASEFY +  KTYDLNFKEENN+GS+KISGD LK++YKSFVT+YPIVSIEDPFDQ
Sbjct: 248 IGMDVAASEFYGSD-KTYDLNFKEENNNGSQKISGDVLKDLYKSFVTEYPIVSIEDPFDQ 307

Query: 307 DDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSVTESIEA 366
           DDWE+YAKLTSEIG +VQIVGDDLLVTNPKRVEKAIKEKACN+LLLKVNQIGSVTESIEA
Sbjct: 308 DDWEHYAKLTSEIGVKVQIVGDDLLVTNPKRVEKAIKEKACNALLLKVNQIGSVTESIEA 367

Query: 367 VKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQLLRIEEE 426
           VK+SK AGWGVMASHRSGETEDTFIADLSVGL+TGQIKTGAPCRSERLAKYNQLLRIEEE
Sbjct: 368 VKMSKRAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQLLRIEEE 427

Query: 427 LGPAAIYAGSKFRSPVEPY 446
           LG  A+YAG+ FR+PVEPY
Sbjct: 428 LGAEAVYAGANFRTPVEPY 445

BLAST of Cp4.1LG14g05720 vs. ExPASy Swiss-Prot
Match: P26300 (Enolase OS=Solanum lycopersicum OX=4081 GN=PGH1 PE=2 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 4.5e-223
Identity = 386/445 (86.74%), Postives = 413/445 (92.81%), Query Frame = 0

Query: 1   MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60
           MA I+ +KARQIFDSRGNPTVEVD+ +++G  ARAAVPSGASTGIYEALELRDGGSDYLG
Sbjct: 1   MATIKSIKARQIFDSRGNPTVEVDVHISNGVFARAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120
           KGV +AV NVNSIIGPAL+GKDP +QT LDNFMV QLDGT NEWGWCK+KLGANAILAVS
Sbjct: 61  KGVSKAVNNVNSIIGPALVGKDPTDQTGLDNFMVHQLDGTQNEWGWCKEKLGANAILAVS 120

Query: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LA+CKAGAAV  +PLYKHIA LAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAVCKAGAAVRNVPLYKHIADLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240
           ++FKEAMKMG EVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AI KAG
Sbjct: 181 ANFKEAMKMGCEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300
           YTGKVVIGMDVAASEFY  K K+YDLNFKEE+NDGS+KISGD LK++YKSFV++YPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFY-GKDKSYDLNFKEESNDGSQKISGDQLKDLYKSFVSEYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWE YAKLT+EIG QVQIVGDDLLVTNPKRV KAI EK CN+LLLKVNQIGSV
Sbjct: 301 EDPFDQDDWETYAKLTAEIGEQVQIVGDDLLVTNPKRVAKAIAEKTCNALLLKVNQIGSV 360

Query: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420
           TESIEAVK+SK AGWGVM SHRSGETEDTFIADL+VGLSTGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKMSKKAGWGVMTSHRSGETEDTFIADLAVGLSTGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGSKFRSPVEPY 446
           LRIEEELG  A+YAG+ FR PVEPY
Sbjct: 421 LRIEEELGSEAVYAGASFRKPVEPY 444

BLAST of Cp4.1LG14g05720 vs. NCBI nr
Match: XP_023552596.1 (enolase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 873 bits (2256), Expect = 0.0
Identity = 445/445 (100.00%), Postives = 445/445 (100.00%), Query Frame = 0

Query: 1   MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60
           MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG
Sbjct: 1   MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300
           YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV
Sbjct: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360

Query: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420
           TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445
           LRIEEELGPAAIYAGSKFRSPVEPY
Sbjct: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445

BLAST of Cp4.1LG14g05720 vs. NCBI nr
Match: XP_022922793.1 (enolase-like [Cucurbita moschata])

HSP 1 Score: 873 bits (2255), Expect = 0.0
Identity = 444/445 (99.78%), Postives = 445/445 (100.00%), Query Frame = 0

Query: 1   MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60
           MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG
Sbjct: 1   MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LA+CKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAVCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300
           YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV
Sbjct: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360

Query: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420
           TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445
           LRIEEELGPAAIYAGSKFRSPVEPY
Sbjct: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445

BLAST of Cp4.1LG14g05720 vs. NCBI nr
Match: KAG6576780.1 (Enolase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014813.1 Enolase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 871 bits (2251), Expect = 0.0
Identity = 443/445 (99.55%), Postives = 444/445 (99.78%), Query Frame = 0

Query: 1   MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60
           MA IEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG
Sbjct: 1   MATIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LA+CKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAVCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300
           YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV
Sbjct: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360

Query: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420
           TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445
           LRIEEELGPAAIYAGSKFRSPVEPY
Sbjct: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445

BLAST of Cp4.1LG14g05720 vs. NCBI nr
Match: XP_022984196.1 (enolase 2-like [Cucurbita maxima])

HSP 1 Score: 865 bits (2236), Expect = 0.0
Identity = 442/445 (99.33%), Postives = 442/445 (99.33%), Query Frame = 0

Query: 1   MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60
           MA IEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGS YLG
Sbjct: 1   MATIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSAYLG 60

Query: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMV QLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVWQLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300
           YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV
Sbjct: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360

Query: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420
           TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445
           LRIEEELGPAAIYAGSKFRSPVEPY
Sbjct: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445

BLAST of Cp4.1LG14g05720 vs. NCBI nr
Match: XP_022141329.1 (enolase-like [Momordica charantia])

HSP 1 Score: 848 bits (2190), Expect = 1.73e-309
Identity = 427/445 (95.96%), Postives = 442/445 (99.33%), Query Frame = 0

Query: 1   MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60
           MA I+IVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG
Sbjct: 1   MATIKIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVL+AV+NVNSIIGPALIGKDPREQT+LDNFMV++LDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLKAVDNVNSIIGPALIGKDPREQTELDNFMVQKLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK+AIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300
           YTGKVVIGMDVAASEFYD+K KTYDLNFKEE+NDGSEKISGDSLKNVYKSFVTDYP+VSI
Sbjct: 241 YTGKVVIGMDVAASEFYDSKDKTYDLNFKEEHNDGSEKISGDSLKNVYKSFVTDYPVVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWE+YAKLTSEIG+QVQIVGDDLLVTNPKRV+KAIKEK CNSLLLKVNQIGSV
Sbjct: 301 EDPFDQDDWEHYAKLTSEIGQQVQIVGDDLLVTNPKRVDKAIKEKTCNSLLLKVNQIGSV 360

Query: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420
           TESIEAVK+SKHAGWGVMASHRSGETEDTFIADLSVGL+TGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445
           LRIEEELGPAAIYAGSKFRSPVEPY
Sbjct: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445

BLAST of Cp4.1LG14g05720 vs. ExPASy TrEMBL
Match: A0A6J1E7U0 (Phosphopyruvate hydratase OS=Cucurbita moschata OX=3662 GN=LOC111430677 PE=3 SV=1)

HSP 1 Score: 873 bits (2255), Expect = 0.0
Identity = 444/445 (99.78%), Postives = 445/445 (100.00%), Query Frame = 0

Query: 1   MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60
           MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG
Sbjct: 1   MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LA+CKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAVCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300
           YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV
Sbjct: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360

Query: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420
           TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445
           LRIEEELGPAAIYAGSKFRSPVEPY
Sbjct: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445

BLAST of Cp4.1LG14g05720 vs. ExPASy TrEMBL
Match: A0A6J1J4K4 (Phosphopyruvate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111482586 PE=3 SV=1)

HSP 1 Score: 865 bits (2236), Expect = 0.0
Identity = 442/445 (99.33%), Postives = 442/445 (99.33%), Query Frame = 0

Query: 1   MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60
           MA IEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGS YLG
Sbjct: 1   MATIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSAYLG 60

Query: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMV QLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVWQLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300
           YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV
Sbjct: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360

Query: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420
           TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445
           LRIEEELGPAAIYAGSKFRSPVEPY
Sbjct: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445

BLAST of Cp4.1LG14g05720 vs. ExPASy TrEMBL
Match: A0A6J1CIC4 (Phosphopyruvate hydratase OS=Momordica charantia OX=3673 GN=LOC111011749 PE=3 SV=1)

HSP 1 Score: 848 bits (2190), Expect = 8.39e-310
Identity = 427/445 (95.96%), Postives = 442/445 (99.33%), Query Frame = 0

Query: 1   MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60
           MA I+IVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG
Sbjct: 1   MATIKIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVL+AV+NVNSIIGPALIGKDPREQT+LDNFMV++LDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLKAVDNVNSIIGPALIGKDPREQTELDNFMVQKLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK+AIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300
           YTGKVVIGMDVAASEFYD+K KTYDLNFKEE+NDGSEKISGDSLKNVYKSFVTDYP+VSI
Sbjct: 241 YTGKVVIGMDVAASEFYDSKDKTYDLNFKEEHNDGSEKISGDSLKNVYKSFVTDYPVVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWE+YAKLTSEIG+QVQIVGDDLLVTNPKRV+KAIKEK CNSLLLKVNQIGSV
Sbjct: 301 EDPFDQDDWEHYAKLTSEIGQQVQIVGDDLLVTNPKRVDKAIKEKTCNSLLLKVNQIGSV 360

Query: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420
           TESIEAVK+SKHAGWGVMASHRSGETEDTFIADLSVGL+TGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445
           LRIEEELGPAAIYAGSKFRSPVEPY
Sbjct: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445

BLAST of Cp4.1LG14g05720 vs. ExPASy TrEMBL
Match: A0A5B7BVG7 (Phosphopyruvate hydratase (Fragment) OS=Davidia involucrata OX=16924 GN=Din_041547 PE=3 SV=1)

HSP 1 Score: 824 bits (2129), Expect = 4.77e-300
Identity = 414/445 (93.03%), Postives = 430/445 (96.63%), Query Frame = 0

Query: 1   MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60
           MA I+ VKARQIFDSRGNPTVEVD+ L+DGTLARAAVPSGASTGIYEALELRDGGSDYLG
Sbjct: 29  MATIKAVKARQIFDSRGNPTVEVDVILSDGTLARAAVPSGASTGIYEALELRDGGSDYLG 88

Query: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVL+AV NVNSIIGPALIGKDP EQTK+DNFMV+QLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 89  KGVLKAVGNVNSIIGPALIGKDPTEQTKIDNFMVQQLDGTVNEWGWCKQKLGANAILAVS 148

Query: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LA+CKAGA VNKIPLYKHIA LAGNK LVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 149 LAVCKAGAIVNKIPLYKHIANLAGNKTLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 208

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240
           +SFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AIAKAG
Sbjct: 209 ASFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIAKAG 268

Query: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300
           YTGKVVIGMDVAASEFYDN  KTYDLNFKEENNDGS+KISGDSLKNVYKSFVTDYPIVSI
Sbjct: 269 YTGKVVIGMDVAASEFYDNNDKTYDLNFKEENNDGSQKISGDSLKNVYKSFVTDYPIVSI 328

Query: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWE+YAKLT EIG QVQIVGDDLLVTNPKRVEKAIKEK CN+LLLKVNQIGSV
Sbjct: 329 EDPFDQDDWEHYAKLTCEIGEQVQIVGDDLLVTNPKRVEKAIKEKTCNALLLKVNQIGSV 388

Query: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420
           TESIEAVK+SKHAGWGVMASHRSGETEDTFIADLSVGL+TGQIKTGAPCRSERLAKYNQL
Sbjct: 389 TESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 448

Query: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445
           LRIEEELG AA+YAG+KFR+PVEPY
Sbjct: 449 LRIEEELGSAAVYAGAKFRAPVEPY 473

BLAST of Cp4.1LG14g05720 vs. ExPASy TrEMBL
Match: A0A200QB48 (Phosphopyruvate hydratase OS=Macleaya cordata OX=56857 GN=BVC80_1827g52 PE=3 SV=1)

HSP 1 Score: 823 bits (2125), Expect = 6.74e-300
Identity = 414/445 (93.03%), Postives = 432/445 (97.08%), Query Frame = 0

Query: 1   MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60
           MA I+ VKARQIFDSRGNPTVEVD+TL+DGTLARAAVPSGASTGIYEALELRDGGSDYLG
Sbjct: 1   MATIKAVKARQIFDSRGNPTVEVDVTLSDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVL+AVENVN IIGPALIGKDP +Q +LDNFMV+QLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLKAVENVNGIIGPALIGKDPTKQVELDNFMVQQLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LAICKAGA+VNKIPLYKHIA LAGNK LVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAICKAGASVNKIPLYKHIANLAGNKTLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300
           YTGKVVIGMDVAASEFYD+K KTYDLNFKEENNDGS+KISGDSLKNVYKSFV DYPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFYDDKDKTYDLNFKEENNDGSQKISGDSLKNVYKSFVADYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWE+YAK+TSEIG+QVQIVGDDLLVTNPKRVEKAIKEK CN+LLLKVNQIGSV
Sbjct: 301 EDPFDQDDWEHYAKMTSEIGQQVQIVGDDLLVTNPKRVEKAIKEKTCNALLLKVNQIGSV 360

Query: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420
           TESIEAVK+SK AGWGVMASHRSGETEDTFIADLSVGL+TGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKMSKQAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445
           LRIEEELG AAIYAG+KFR+PVEPY
Sbjct: 421 LRIEEELGSAAIYAGAKFRTPVEPY 445

BLAST of Cp4.1LG14g05720 vs. TAIR 10
Match: AT2G36530.1 (Enolase )

HSP 1 Score: 762.7 bits (1968), Expect = 1.7e-220
Identity = 382/445 (85.84%), Postives = 411/445 (92.36%), Query Frame = 0

Query: 1   MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60
           MA I +VKARQIFDSRGNPTVEVDI  ++G    AAVPSGASTGIYEALELRDGGSDYLG
Sbjct: 1   MATITVVKARQIFDSRGNPTVEVDIHTSNGIKVTAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120
           KGV +AV NVN+IIGPALIGKDP +QT +DNFMV +LDGT NEWGWCKQKLGANAILAVS
Sbjct: 61  KGVSKAVGNVNNIIGPALIGKDPTQQTAIDNFMVHELDGTQNEWGWCKQKLGANAILAVS 120

Query: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LA+CKAGA V+ IPLYKHIA LAGN K+VLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAVCKAGAVVSGIPLYKHIANLAGNPKIVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240
           +SFKEAMKMGVEVYHHLK+VIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AI KAG
Sbjct: 181 ASFKEAMKMGVEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300
           YTGKVVIGMDVAASEFY ++ KTYDLNFKEENN+GS+KISGD+LK++YKSFV +YPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFY-SEDKTYDLNFKEENNNGSQKISGDALKDLYKSFVAEYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWE+YAK+T+E G +VQIVGDDLLVTNPKRV KAI EK+CN+LLLKVNQIGSV
Sbjct: 301 EDPFDQDDWEHYAKMTTECGTEVQIVGDDLLVTNPKRVAKAIAEKSCNALLLKVNQIGSV 360

Query: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420
           TESIEAVK+SK AGWGVM SHRSGETEDTFIADL+VGLSTGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKMSKKAGWGVMTSHRSGETEDTFIADLAVGLSTGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGSKFRSPVEPY 446
           LRIEEELG  AIYAG  FR PVEPY
Sbjct: 421 LRIEEELGSEAIYAGVNFRKPVEPY 444

BLAST of Cp4.1LG14g05720 vs. TAIR 10
Match: AT1G74030.1 (enolase 1 )

HSP 1 Score: 577.8 bits (1488), Expect = 7.6e-165
Identity = 301/436 (69.04%), Postives = 354/436 (81.19%), Query Frame = 0

Query: 7   VKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGG-SDYLGKGVLE 66
           VKARQI DSRGNPTVEVD+   D  L R+AVPSGASTGIYEALELRDG  S Y GKGVL+
Sbjct: 54  VKARQIIDSRGNPTVEVDLITDD--LYRSAVPSGASTGIYEALELRDGDKSVYGGKGVLQ 113

Query: 67  AVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVSLAICK 126
           A++N+N ++ P LIG D R Q  +D  M+ +LDGT N     K KLGANAIL VSL++C+
Sbjct: 114 AIKNINELVAPKLIGVDVRNQADVDALML-ELDGTPN-----KSKLGANAILGVSLSVCR 173

Query: 127 AGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKE 186
           AGA    +PLYKHI + +G K+LV+PVPAFNVINGGSHAGN LAMQEFMILPVGA+SF E
Sbjct: 174 AGAGAKGVPLYKHIQETSGTKELVMPVPAFNVINGGSHAGNSLAMQEFMILPVGATSFSE 233

Query: 187 AMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAGYTGKV 246
           A +MG EVYH LK +IK KYGQDA NVGDEGGFAPN+Q+N+EGL LL  AI KAGYTGK+
Sbjct: 234 AFQMGSEVYHTLKGIIKTKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 293

Query: 247 VIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSIEDPFD 306
            IGMDVAASEF+  K   YDLNFK++ NDG+  +S +SL ++Y+ F+ D+PIVSIEDPFD
Sbjct: 294 KIGMDVAASEFF-MKDGRYDLNFKKQPNDGAHVLSAESLADLYREFIKDFPIVSIEDPFD 353

Query: 307 QDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSVTESIE 366
           QDDW ++A L S +   +Q+VGDDLLVTNPKR+ +AIK+++CN+LLLKVNQIG+VTESI+
Sbjct: 354 QDDWSSWASLQSSV--DIQLVGDDLLVTNPKRIAEAIKKQSCNALLLKVNQIGTVTESIQ 413

Query: 367 AVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQLLRIEE 426
           A   SK AGWGVM SHRSGETED FIADLSVGL++GQIKTGAPCRSERL+KYNQLLRIEE
Sbjct: 414 AALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLSKYNQLLRIEE 473

Query: 427 ELGPAAIYAGSKFRSP 442
           ELG    YAG  FRSP
Sbjct: 474 ELGNVR-YAGEAFRSP 477

BLAST of Cp4.1LG14g05720 vs. TAIR 10
Match: AT2G29560.1 (cytosolic enolase )

HSP 1 Score: 468.0 bits (1203), Expect = 8.4e-132
Identity = 250/439 (56.95%), Postives = 315/439 (71.75%), Query Frame = 0

Query: 2   AAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSD-YLG 61
           + I  VKARQI DSRG PTVEVD+    G   RA+VPSG S+G YEA+ELRDG    YLG
Sbjct: 45  SVITKVKARQILDSRGIPTVEVDLHTNKGVF-RASVPSGDSSGTYEAIELRDGDKGMYLG 104

Query: 62  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 121
             V +AV+N+N  I  ALIG DP+ Q ++D  M+  LD T       K +LGANAILAVS
Sbjct: 105 NSVAKAVKNINEKISEALIGMDPKLQGQIDQAMI-DLDKTEK-----KSELGANAILAVS 164

Query: 122 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 181
           +A CKAGAA  ++PL KH++ L+G   +VLPVPAF V++GG HA N  A+QE MILP+GA
Sbjct: 165 IAACKAGAAEKEVPLCKHLSDLSGRANMVLPVPAFTVLSGGKHASNTFAIQEIMILPIGA 224

Query: 182 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 241
           S F+EA++ G E YHHLKAVI +K G    NVG++GG AP+I   KEGLEL+K AI + G
Sbjct: 225 SRFEEALQWGSETYHHLKAVISEKNGGLGCNVGEDGGLAPDISSLKEGLELVKEAINRTG 284

Query: 242 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 301
           Y  K+ I +D+AA+ F       YDL+ K  N  G    S + + ++YK    DYPIVSI
Sbjct: 285 YNDKIKIAIDIAATNFC--LGTKYDLDIKSPNKSGQNFKSAEDMIDMYKEICNDYPIVSI 344

Query: 302 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 361
           EDPFD++DWE + K  S +G   Q+VGDDLL++N KRVE+AI+E +CN+LLLKVNQIG+V
Sbjct: 345 EDPFDKEDWE-HTKYFSSLG-ICQVVGDDLLMSNSKRVERAIQESSCNALLLKVNQIGTV 404

Query: 362 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 421
           TE+IE VK+++ A WGV+ SHR GETED+FI+DLSVGL+TG IK GAPCR ER  KYNQL
Sbjct: 405 TEAIEVVKMARDAQWGVVTSHRCGETEDSFISDLSVGLATGVIKAGAPCRGERTMKYNQL 464

Query: 422 LRIEEELGPAAIYAGSKFR 440
           LRIEEELG  A+YAG  ++
Sbjct: 465 LRIEEELGDQAVYAGEDWK 472

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q429719.8e-22687.39Enolase OS=Oryza sativa subsp. japonica OX=39947 GN=ENO1 PE=1 SV=2[more]
P428961.7e-22589.29Enolase OS=Ricinus communis OX=3988 PE=2 SV=1[more]
P428954.9e-22586.94Enolase 2 OS=Zea mays OX=4577 GN=ENO2 PE=2 SV=1[more]
Q9LEI99.2e-22488.61Enolase 2 OS=Hevea brasiliensis OX=3981 GN=ENO2 PE=1 SV=1[more]
P263004.5e-22386.74Enolase OS=Solanum lycopersicum OX=4081 GN=PGH1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023552596.10.0100.00enolase-like [Cucurbita pepo subsp. pepo][more]
XP_022922793.10.099.78enolase-like [Cucurbita moschata][more]
KAG6576780.10.099.55Enolase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014813.1 Enolase, ... [more]
XP_022984196.10.099.33enolase 2-like [Cucurbita maxima][more]
XP_022141329.11.73e-30995.96enolase-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1E7U00.099.78Phosphopyruvate hydratase OS=Cucurbita moschata OX=3662 GN=LOC111430677 PE=3 SV=... [more]
A0A6J1J4K40.099.33Phosphopyruvate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111482586 PE=3 SV=1[more]
A0A6J1CIC48.39e-31095.96Phosphopyruvate hydratase OS=Momordica charantia OX=3673 GN=LOC111011749 PE=3 SV... [more]
A0A5B7BVG74.77e-30093.03Phosphopyruvate hydratase (Fragment) OS=Davidia involucrata OX=16924 GN=Din_0415... [more]
A0A200QB486.74e-30093.03Phosphopyruvate hydratase OS=Macleaya cordata OX=56857 GN=BVC80_1827g52 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT2G36530.11.7e-22085.84Enolase [more]
AT1G74030.17.6e-16569.04enolase 1 [more]
AT2G29560.18.4e-13256.95cytosolic enolase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000941EnolasePRINTSPR00148ENOLASEcoord: 379..396
score: 83.84
coord: 327..338
score: 75.51
coord: 350..364
score: 80.2
coord: 112..128
score: 79.68
coord: 169..182
score: 77.06
coord: 37..51
score: 88.38
IPR000941EnolasePIRSFPIRSF001400Enolasecoord: 1..440
e-value: 2.6E-190
score: 630.5
IPR000941EnolaseTIGRFAMTIGR01060TIGR01060coord: 4..439
e-value: 4.3E-174
score: 577.1
IPR000941EnolaseSFLDSFLDF00002enolasecoord: 2..427
e-value: 0.0
score: 641.4
IPR000941EnolasePANTHERPTHR11902ENOLASEcoord: 1..445
IPR000941EnolaseHAMAPMF_00318Enolasecoord: 2..427
score: 44.375076
IPR000941EnolaseCDDcd03313enolasecoord: 7..426
e-value: 0.0
score: 752.007
IPR020811Enolase, N-terminalSMARTSM01193Enolase_N_3coord: 4..139
e-value: 2.0E-81
score: 286.5
IPR020811Enolase, N-terminalPFAMPF03952Enolase_Ncoord: 4..139
e-value: 2.6E-52
score: 176.5
IPR020810Enolase, C-terminal TIM barrel domainSMARTSM01192Enolase_C_3coord: 147..441
e-value: 7.7E-204
score: 693.1
IPR020810Enolase, C-terminal TIM barrel domainPFAMPF00113Enolase_Ccoord: 148..440
e-value: 8.2E-157
score: 521.0
IPR029017Enolase-like, N-terminalGENE3D3.30.390.10coord: 1..131
e-value: 1.7E-58
score: 198.5
IPR029017Enolase-like, N-terminalSUPERFAMILY54826Enolase N-terminal domain-likecoord: 4..143
IPR036849Enolase-like, C-terminal domain superfamilyGENE3D3.20.20.120coord: 133..444
e-value: 3.4E-164
score: 547.3
IPR036849Enolase-like, C-terminal domain superfamilySUPERFAMILY51604Enolase C-terminal domain-likecoord: 147..440
NoneNo IPR availableSFLDSFLDG00178enolasecoord: 2..427
e-value: 0.0
score: 641.4
NoneNo IPR availablePANTHERPTHR11902:SF41ENOLASEcoord: 1..445
IPR020809Enolase, conserved sitePROSITEPS00164ENOLASEcoord: 350..363

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG14g05720.1Cp4.1LG14g05720.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
cellular_component GO:0000015 phosphopyruvate hydratase complex
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0004634 phosphopyruvate hydratase activity