Cp4.1LG14g05530 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG14g05530
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionNADPH-dependent aldo-keto reductase, chloroplastic
LocationCp4.1LG14: 929304 .. 932155 (-)
RNA-Seq ExpressionCp4.1LG14g05530
SyntenyCp4.1LG14g05530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTAGGAAGATGATGGGGTTGGGGCTCTATTCTTCTAGGTTTTGCCAATTAAATGTTTCACTATTAAATATAACCAACTTGTTGACTTGTTTCTAAGAAAATTCCATGACACCTAGGAGAGAAATAAAGAACACAAAACCAAGTAATCCTGTCCCTTGGATGACAAGATCAAGGGCCAATCCAACGGTATCCCACAAAAGATGGAAAATAGATCCCACAAACTAGGCACTAAATTTTTCAGACACAAACATCAATGATTCATTCATCATAAGCTGTAATGTCCTGTTAGGAGACAGTTAACAACGTTGACTTCAACGCTCTCTCTCCTATATAAACACATCTTCTAACCTAATCTCTCTCCAAATTATTATTACATAATTTAGAGAGAGAAAGAGAAGAACACAATGGAAAATCAAGTGAGATTGAATTGTGGTGTAAATCTTCCCATTTTTGGTTTTGGCACTTTTTCTTATCAAGCTCACTCAAAAGCCATAGAATCAGCTGTTCATATGGCCATCAAAGTACATATTGATAAGCTCTTTTTCTCTTCATTTTTGTACCTTTTTCCATTGTTTCTTACCTTATTTTTCTTTGATTTTTCAGATGGGTCATAGGCATTTTGATACAGCAACTATCTATGGATCTGAGGGGGCTGTTGGCAAAGCCATTTGTGATGAAATTTTGAGCCAAGATGTGGGGAGAGAAGACATTTTTATCACTACAAAAGTATGGGGAAATGACCATCATGATCCTGTTTCAGCATTAAAACAGTCACTAAGGTAATCTATTACATAATTACAAGGCTAAAGCTTCGATTTTTGTCGAACCCACGTTGAAATTCGATAGATTAAAGCTTATACAATCACGTTATAGTAACGGGTTTTGTTTTAAAACGCGAAATTGTCGTTGAATTTTGAATTTTGGTGTTTATAGGAACATGGGATTGGAGTATATTGATATGTATTTGGTGCATTGGCCATTGAAGCTGAAGCCTTGGGCATGTTATCCAGTTCCCAAGGAAAGTGATTTTGAGGAAGCATTGGATCTCGAAAGCACTTGGAAGGGAATGGAAAAGTGCCAAGAATTGGGTCTGTGTAGGTGCATTGGTGTGAGCAATTTCTCCACCAAAAAACTTGAATCCCTTTTAGAATTTGCTTCCATTCCTCCAGCTGTTAACCAGGTTTCTCTCTCTCTTTAGCTTCTATTAATGTTATTAATTGGTCTGTTTTTTGGATTTTTGTTCTTGAATTAACATGATGTTTACAATATTGAAAGTCAATCTAGTGGGGTTTAATGTTGATGAATAATCACCTAAGTTCAAGAGCTAGTTAGGATAATAAAAGCTTAGGAGTGTCGAACCGTCGTTTCCAATTTCACTCGATTACATGTCTGTCTTGTATATTTGTGAGCTTTCACATCGGTTGGGGAGGAGAACGAATGATCTTTTACAAGGGTGTGAAAACTTCTTTCTAGTAGACAAGTTTTAAAATTGTGAGGCTGATGATGATATTTAATGGGCCAAAGTGGATAATATCTGCTAACGGTGAGTTTGGGTTGTTACAAATGGTATTAGAGCCAGACACCAGGAAGTGTGTCAGGGAGAACGTTGGGACTCCAAATGGGGTGGATTGTGAGATCCTACATCGGTTGGAGAGGGGATCAGAGCCTTCCTTATACAAGTGTGAAAACCTTTCCTTAATAGACACGTTTTAAAACCGTGAGACTGACATCAATACGTAACATGCGACAATATCCGCTAGGGTTGACTACAACAAGAAAACAAGTTTGATGTCATCTAATTTATTAGAAAGTAAAGACTAAAAATAGAATATTTATAATTTTAGAGACCAAATTGGGGACAATAGTATCATTATTAAGATCTTCCTATCCTAATAAATATTTCTTCATTATTTTTTTTCAATTTGTATAGTAAATTTATAGTATAATAGGGAAGTTTGATACTCCGAGATACTCGAAACCAAGAGAATCCCGAGTTAGAATGAGATTTCTAGAACCCAAAATAGAAAATTAGATACGATAAATAAATAACGTGAAACAAAAATGTTATCAATTAAGCCAGCCTCAATGACTTAGAACTTCAAGAAGATGGTAGTCTCAAACAAACCGTCCTTTGAATGAGTATCTACAAGGAGACAATATCGTTAGGATGTATAACCAAAAGCAGCTGAATGGTGAAAAACGTATCTAACTTCTTTCATAACGTTGAAAGTTCGATCTCGAGATTCGTTCAATTCTTTTTAAAAAAAAACCCTAATTTGTTGAATGAATAGGTAGAGATGCATCCAATGTGGAGACAAAGAAAGCTAAGAGAGTTTTGTGGAGAGCATAAGATCCACGTGAGTGCATATTCTCCATTGGGTGGACCAGGGAATGCATGGGGATCAACGGCTGTGATTGAAAGTCCACTCATCAAATCAATCGCTCTCAAACACAATGCAACTCCAGCTCAGGTTATTTCAAAAACTTGTCTCTTGTCTCCCAAGTCCTAGGTTAGGATAATTAATAATTCTTTTAATGAAATTGAAACTTTACGATAAACGTTAATACATTTTATCACGTCTCGACTAACACGTTTTTTTTTTTTGTTATATACGTTGAAGGTTGCTCTGCAGTGGGGCCTATCGAAAGGATCGAGTGTGATAGTGAAGAGCTTTAACGAACACCGTATGAAAGAAAACTTTGGAGCCCTTGATTTAAAATTGGACGATCAAGATTGCTTGGAAATTGAAAAATTAGAAGAAAGAAAGATTATGAGGGGTGAATATTTTGTAAACGACACAACAAGTCCATTCAAAATTATTCAAGATTTGTGGGACGGAGAAATTTAA

mRNA sequence

ATGGAGAAAGAGAAGAACACAATGGAAAATCAAGTGAGATTGAATTGTGGTGTAAATCTTCCCATTTTTGGTTTTGGCACTTTTTCTTATCAAGCTCACTCAAAAGCCATAGAATCAGCTGTTCATATGGCCATCAAAATGGGTCATAGGCATTTTGATACAGCAACTATCTATGGATCTGAGGGGGCTGTTGGCAAAGCCATTTGTGATGAAATTTTGAGCCAAGATGTGGGGAGAGAAGACATTTTTATCACTACAAAAGTATGGGGAAATGACCATCATGATCCTGTTTCAGCATTAAAACAGTCACTAAGGAACATGGGATTGGAGTATATTGATATGTATTTGGTGCATTGGCCATTGAAGCTGAAGCCTTGGGCATGTTATCCAGTTCCCAAGGAAAGTGATTTTGAGGAAGCATTGGATCTCGAAAGCACTTGGAAGGGAATGGAAAAGTGCCAAGAATTGGGTCTGTGTAGGTGCATTGGTGTGAGCAATTTCTCCACCAAAAAACTTGAATCCCTTTTAGAATTTGCTTCCATTCCTCCAGCTGTTAACCAGGTAGAGATGCATCCAATGTGGAGACAAAGAAAGCTAAGAGAGTTTTGTGGAGAGCATAAGATCCACGTGAGTGCATATTCTCCATTGGGTGGACCAGGGAATGCATGGGGATCAACGGCTGTGATTGAAAGTCCACTCATCAAATCAATCGCTCTCAAACACAATGCAACTCCAGCTCAGGTTGCTCTGCAGTGGGGCCTATCGAAAGGATCGAGTGTGATAGTGAAGAGCTTTAACGAACACCGTATGAAAGAAAACTTTGGAGCCCTTGATTTAAAATTGGACGATCAAGATTGCTTGGAAATTGAAAAATTAGAAGAAAGAAAGATTATGAGGGGTGAATATTTTGTAAACGACACAACAAGTCCATTCAAAATTATTCAAGATTTGTGGGACGGAGAAATTTAA

Coding sequence (CDS)

ATGGAGAAAGAGAAGAACACAATGGAAAATCAAGTGAGATTGAATTGTGGTGTAAATCTTCCCATTTTTGGTTTTGGCACTTTTTCTTATCAAGCTCACTCAAAAGCCATAGAATCAGCTGTTCATATGGCCATCAAAATGGGTCATAGGCATTTTGATACAGCAACTATCTATGGATCTGAGGGGGCTGTTGGCAAAGCCATTTGTGATGAAATTTTGAGCCAAGATGTGGGGAGAGAAGACATTTTTATCACTACAAAAGTATGGGGAAATGACCATCATGATCCTGTTTCAGCATTAAAACAGTCACTAAGGAACATGGGATTGGAGTATATTGATATGTATTTGGTGCATTGGCCATTGAAGCTGAAGCCTTGGGCATGTTATCCAGTTCCCAAGGAAAGTGATTTTGAGGAAGCATTGGATCTCGAAAGCACTTGGAAGGGAATGGAAAAGTGCCAAGAATTGGGTCTGTGTAGGTGCATTGGTGTGAGCAATTTCTCCACCAAAAAACTTGAATCCCTTTTAGAATTTGCTTCCATTCCTCCAGCTGTTAACCAGGTAGAGATGCATCCAATGTGGAGACAAAGAAAGCTAAGAGAGTTTTGTGGAGAGCATAAGATCCACGTGAGTGCATATTCTCCATTGGGTGGACCAGGGAATGCATGGGGATCAACGGCTGTGATTGAAAGTCCACTCATCAAATCAATCGCTCTCAAACACAATGCAACTCCAGCTCAGGTTGCTCTGCAGTGGGGCCTATCGAAAGGATCGAGTGTGATAGTGAAGAGCTTTAACGAACACCGTATGAAAGAAAACTTTGGAGCCCTTGATTTAAAATTGGACGATCAAGATTGCTTGGAAATTGAAAAATTAGAAGAAAGAAAGATTATGAGGGGTGAATATTTTGTAAACGACACAACAAGTCCATTCAAAATTATTCAAGATTTGTGGGACGGAGAAATTTAA

Protein sequence

MEKEKNTMENQVRLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKAICDEILSQDVGREDIFITTKVWGNDHHDPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWACYPVPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQVEMHPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQVALQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDTTSPFKIIQDLWDGEI
Homology
BLAST of Cp4.1LG14g05530 vs. ExPASy Swiss-Prot
Match: Q0PGJ6 (NADPH-dependent aldo-keto reductase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AKR4C9 PE=1 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 5.8e-79
Identity = 143/312 (45.83%), Postives = 200/312 (64.10%), Query Frame = 0

Query: 13  RLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKAICDEI 72
           +LN G   P  G GT  +QA    +  AV  A+K+G+RH D A IYG+E  +G  +    
Sbjct: 9   KLNTGAKFPSVGLGT--WQASPGLVGDAVAAAVKIGYRHIDCAQIYGNEKEIGAVLKKLF 68

Query: 73  LSQDVGREDIFITTKVWGNDH--HDPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWACYP 132
             + V RED+FIT+K+W  DH   D   AL ++L+++ LEY+D+YL+HWP ++K  +   
Sbjct: 69  EDRVVKREDLFITSKLWCTDHDPQDVPEALNRTLKDLQLEYVDLYLIHWPARIKKGSVGI 128

Query: 133 VPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQVEM 192
            P+       +D+ STWK ME   + G  R IGVSNFSTKKL  LLE A +PPAVNQVE 
Sbjct: 129 KPENL---LPVDIPSTWKAMEALYDSGKARAIGVSNFSTKKLADLLELARVPPAVNQVEC 188

Query: 193 HPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQVAL 252
           HP WRQ KL+EFC    +H+SAYSPLG PG  W  + V+++P++  +A K   +PAQVAL
Sbjct: 189 HPSWRQTKLQEFCKSKGVHLSAYSPLGSPGTTWLKSDVLKNPILNMVAEKLGKSPAQVAL 248

Query: 253 QWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDTTSP 312
           +WGL  G SV+ KS NE R+KENF   D  + D    +  ++E+ +++ G + V++T SP
Sbjct: 249 RWGLQMGHSVLPKSTNEGRIKENFNVFDWSIPDYMFAKFAEIEQARLVTGSFLVHETLSP 308

Query: 313 FKIIQDLWDGEI 323
           +K I++LWDGEI
Sbjct: 309 YKSIEELWDGEI 315

BLAST of Cp4.1LG14g05530 vs. ExPASy Swiss-Prot
Match: Q9SQ64 (Non-functional NADPH-dependent codeinone reductase 2 OS=Papaver somniferum OX=3469 GN=COR2 PE=1 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.4e-77
Identity = 142/316 (44.94%), Postives = 215/316 (68.04%), Query Frame = 0

Query: 12  VRLNCGVNLPIFGFGTFSYQAH-SKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKAICD 71
           V L+ G  +PI G GT       S+ ++ A+  AI++G+RHFDTA +Y +EG++G+A+ +
Sbjct: 9   VTLSSGRGMPILGMGTAENNLQGSERVKLAILKAIEVGYRHFDTAFVYQTEGSLGEAVAE 68

Query: 72  EILSQDV-GREDIFITTKVWGNDHHDP--VSALKQSLRNMGLEYIDMYLVHWPLKLKPWA 131
            + +  +  R+++FIT+K+W  D +    + AL+ SLRN+ LEY+D+YL+HWP+ LKP  
Sbjct: 69  ALQNGLIKSRDELFITSKLWCADAYPDHVLPALQNSLRNLKLEYLDLYLIHWPVSLKPGK 128

Query: 132 -CYPVPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVN 191
             +P+PK+  F   +D +S W  MEKCQ LGL + IGVSNFS KKL  L+  A+IPPAVN
Sbjct: 129 FVHPIPKDEIF--PIDYKSVWAAMEKCQMLGLTKSIGVSNFSCKKLHYLMATANIPPAVN 188

Query: 192 QVEMHPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPA 251
           QVEM+P+W+Q+KLR++C  + I V+AYSPLG  G  WGS+ V++S ++  I+     + A
Sbjct: 189 QVEMNPIWQQQKLRDYCKTNNIMVTAYSPLGAKGTMWGSSGVMDSEVLNQISQVRGKSVA 248

Query: 252 QVALQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVND 311
           QV+L+W   +G+S++VKSFNE RMKEN    D +L  +D   I +L +R++  G+ FV+ 
Sbjct: 249 QVSLRWVYEQGASLLVKSFNEERMKENLKIFDWELSPEDLKNISELPQRRVSTGDPFVS- 308

Query: 312 TTSPFKIIQDLWDGEI 323
              PFK +++LWD E+
Sbjct: 309 INGPFKSVEELWDDEV 321

BLAST of Cp4.1LG14g05530 vs. ExPASy Swiss-Prot
Match: B4F9A4 (Deoxymugineic acid synthase 1 OS=Zea mays OX=4577 GN=DMAS1 PE=1 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 5.7e-74
Identity = 141/313 (45.05%), Postives = 202/313 (64.54%), Query Frame = 0

Query: 13  RLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKAICDEI 72
           R  CG  LP  G GT         + +AV  AI++G+RHFDTA  Y +E  +G+A  + +
Sbjct: 6   RAPCG--LPRIGLGTAVQGPRPDPVRAAVLRAIQLGYRHFDTAAHYATEAPIGEAAAEAV 65

Query: 73  LSQDV-GREDIFITTKVWGND-HHDPV-SALKQSLRNMGLEYIDMYLVHWPLKLKPWACY 132
            +  V  RED+F+T+KVW  D H D V  AL+++L N+ +EY+D+Y+VHWP+ +K    +
Sbjct: 66  RTGLVASREDLFVTSKVWCADAHRDRVLPALRRTLSNLQMEYVDLYMVHWPVTMKA-GRF 125

Query: 133 PVPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQVE 192
             P   +  E  D+ + W+ ME+C  LGL + IGV NFS KKLE+LL FA+IPP VNQVE
Sbjct: 126 TAPFTPEDFEPFDMRAVWEAMEECHRLGLAKAIGVCNFSCKKLETLLSFATIPPVVNQVE 185

Query: 193 MHPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQVA 252
           ++P+W+QRKLREFC    I + AYSPLG  G  WGS +V++S ++  IA     T AQV 
Sbjct: 186 INPVWQQRKLREFCRAKGIQLCAYSPLGAKGTHWGSDSVMDSGVLHEIAKSKGKTVAQVC 245

Query: 253 LQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDTTS 312
           L+W   +G  +IVKSF+E RMKEN   +D +L +++   I K+ +RKI +G  +V++   
Sbjct: 246 LRWVYEQGDCLIVKSFDEGRMKENLDIVDWELSEEERQRISKIPQRKINQGRRYVSE-HG 305

Query: 313 PFKIIQDLWDGEI 323
           P+K  ++LWDGEI
Sbjct: 306 PYKSFEELWDGEI 314

BLAST of Cp4.1LG14g05530 vs. ExPASy Swiss-Prot
Match: Q10PE7 (Deoxymugineic acid synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DMAS1 PE=1 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 7.4e-74
Identity = 141/309 (45.63%), Postives = 198/309 (64.08%), Query Frame = 0

Query: 17  GVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKAICDEILSQD 76
           G  +P  G GT       + I  AV  AI+ G+RHFDTA  Y +E  +G+A  + + S  
Sbjct: 12  GFGMPRVGMGTAVQGPRPEPIRRAVLKAIEAGYRHFDTAAHYETEAPIGEAAAEAVRSGA 71

Query: 77  V-GREDIFITTKVWGND-HHDPV-SALKQSLRNMGLEYIDMYLVHWPLKLKPWACYPVPK 136
           +  R D+FIT+K+W +D H D V  AL+Q+L N+ +EY+D+YLVHWP+ +KP   Y  P 
Sbjct: 72  IASRADLFITSKLWCSDAHRDRVLPALRQTLWNLQMEYVDLYLVHWPVSMKP-GRYKAPF 131

Query: 137 ESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQVEMHPM 196
            +D     D+ + W+ ME+C  LGL + IGV NFS KKL++LL FA+IPPAVNQVE++P+
Sbjct: 132 TADDFVPFDMRAVWEAMEECHRLGLAKAIGVCNFSCKKLDTLLSFATIPPAVNQVEVNPV 191

Query: 197 WRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQVALQWG 256
           W+QRKLRE C E  + + AYSPLG  G  WGS +V+ S +++ IA     T AQV L+W 
Sbjct: 192 WQQRKLRELCREKGVQICAYSPLGASGTHWGSDSVMASAVLRDIAQSKGKTVAQVCLRWV 251

Query: 257 LSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDTTSPFKI 316
             +G  +IVKSF+E RM+EN   +  +L +++   I  + +RKI R   FV+D   P+K 
Sbjct: 252 YEQGDCLIVKSFDEARMRENLDIVGWELTEEERQRIAGIPQRKINRALRFVSD-HGPYKS 311

Query: 317 IQDLWDGEI 323
           + DLWDGEI
Sbjct: 312 LDDLWDGEI 318

BLAST of Cp4.1LG14g05530 vs. ExPASy Swiss-Prot
Match: Q9M338 (Aldo-keto reductase family 4 member C11 OS=Arabidopsis thaliana OX=3702 GN=AKR4C11 PE=1 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 2.2e-73
Identity = 137/312 (43.91%), Postives = 198/312 (63.46%), Query Frame = 0

Query: 13  RLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKAICDEI 72
           +LN G  +P  G GT  +QA    +  AV  A+K+G++H D A+ YG+E  +GK +    
Sbjct: 9   QLNTGAKIPSVGLGT--WQAAPGVVGDAVAAAVKIGYQHIDCASRYGNEIEIGKVLKKLF 68

Query: 73  LSQDVGREDIFITTKVWGNDHHDP--VSALKQSLRNMGLEYIDMYLVHWPLKLKPWACYP 132
               V RE +FIT+K+W  D   P    AL ++L+++ L+Y+D+YL+HWP++LK      
Sbjct: 69  DDGVVKREKLFITSKIWLTDLDPPDVQDALNRTLQDLQLDYVDLYLMHWPVRLKKGT--- 128

Query: 133 VPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQVEM 192
           V  + +    +D+ STWK ME   + G  R IGVSNFSTKKL  L+E A +PPAVNQVE 
Sbjct: 129 VDFKPENIMPIDIPSTWKAMEALVDSGKARAIGVSNFSTKKLSDLVEAARVPPAVNQVEC 188

Query: 193 HPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQVAL 252
           HP W+Q KL EFC    IH+S YSPLG PG  W    V++SP+I+ IA +   +PAQ AL
Sbjct: 189 HPSWQQHKLHEFCKSKGIHLSGYSPLGSPGTTWVKADVLKSPVIEMIAKEIGKSPAQTAL 248

Query: 253 QWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDTTSP 312
           +WGL  G S++ KS NE R++ENF  L   +  +   +  K+E+ ++++G  FV++T SP
Sbjct: 249 RWGLQMGHSILPKSTNEGRIRENFDVLGWSIPKEMFDKFSKIEQARLVQGTSFVHETLSP 308

Query: 313 FKIIQDLWDGEI 323
           +K +++LWDGEI
Sbjct: 309 YKTLEELWDGEI 315

BLAST of Cp4.1LG14g05530 vs. NCBI nr
Match: XP_023551746.1 (NADPH-dependent aldo-keto reductase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 660 bits (1702), Expect = 2.16e-239
Identity = 315/315 (100.00%), Postives = 315/315 (100.00%), Query Frame = 0

Query: 8   MENQVRLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKA 67
           MENQVRLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKA
Sbjct: 1   MENQVRLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKA 60

Query: 68  ICDEILSQDVGREDIFITTKVWGNDHHDPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWA 127
           ICDEILSQDVGREDIFITTKVWGNDHHDPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWA
Sbjct: 61  ICDEILSQDVGREDIFITTKVWGNDHHDPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWA 120

Query: 128 CYPVPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQ 187
           CYPVPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQ
Sbjct: 121 CYPVPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQ 180

Query: 188 VEMHPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQ 247
           VEMHPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQ
Sbjct: 181 VEMHPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQ 240

Query: 248 VALQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDT 307
           VALQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDT
Sbjct: 241 VALQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDT 300

Query: 308 TSPFKIIQDLWDGEI 322
           TSPFKIIQDLWDGEI
Sbjct: 301 TSPFKIIQDLWDGEI 315

BLAST of Cp4.1LG14g05530 vs. NCBI nr
Match: KAG7014834.1 (NADPH-dependent aldo-keto reductase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 651 bits (1680), Expect = 4.89e-236
Identity = 310/315 (98.41%), Postives = 314/315 (99.68%), Query Frame = 0

Query: 8   MENQVRLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKA 67
           MENQVRLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKA
Sbjct: 1   MENQVRLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKA 60

Query: 68  ICDEILSQDVGREDIFITTKVWGNDHHDPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWA 127
           ICDEILSQ+VGREDIFITTKVWGNDHH+PVSALKQSLRNMGLEYIDMYLVHWPLKLKPWA
Sbjct: 61  ICDEILSQNVGREDIFITTKVWGNDHHNPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWA 120

Query: 128 CYPVPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQ 187
           CYPVPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLE ASIPPAVNQ
Sbjct: 121 CYPVPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEIASIPPAVNQ 180

Query: 188 VEMHPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQ 247
           VEMHPMWRQRK+REFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQ
Sbjct: 181 VEMHPMWRQRKVREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQ 240

Query: 248 VALQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDT 307
           VALQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDT
Sbjct: 241 VALQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDT 300

Query: 308 TSPFKIIQDLWDGEI 322
           TSP+KIIQDLWDGEI
Sbjct: 301 TSPYKIIQDLWDGEI 315

BLAST of Cp4.1LG14g05530 vs. NCBI nr
Match: XP_022985497.1 (NADPH-dependent aldo-keto reductase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 649 bits (1675), Expect = 2.83e-235
Identity = 308/315 (97.78%), Postives = 313/315 (99.37%), Query Frame = 0

Query: 8   MENQVRLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKA 67
           MENQVRLNCG+NLP FGFGTFSYQAHSK IESAVHMAIKMGHRHFDTATIYGSEGAVGKA
Sbjct: 1   MENQVRLNCGLNLPHFGFGTFSYQAHSKTIESAVHMAIKMGHRHFDTATIYGSEGAVGKA 60

Query: 68  ICDEILSQDVGREDIFITTKVWGNDHHDPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWA 127
           ICDEIL+Q+VGREDIFITTKVWGNDHHDPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWA
Sbjct: 61  ICDEILNQNVGREDIFITTKVWGNDHHDPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWA 120

Query: 128 CYPVPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQ 187
           CYPVPKESDFEEALD+ESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQ
Sbjct: 121 CYPVPKESDFEEALDIESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQ 180

Query: 188 VEMHPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQ 247
           VEMHPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQ
Sbjct: 181 VEMHPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQ 240

Query: 248 VALQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDT 307
           VALQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDT
Sbjct: 241 VALQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDT 300

Query: 308 TSPFKIIQDLWDGEI 322
           TSP+KIIQDLWDGEI
Sbjct: 301 TSPYKIIQDLWDGEI 315

BLAST of Cp4.1LG14g05530 vs. NCBI nr
Match: XP_022923116.1 (NADPH-dependent aldo-keto reductase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 648 bits (1672), Expect = 8.11e-235
Identity = 309/315 (98.10%), Postives = 313/315 (99.37%), Query Frame = 0

Query: 8   MENQVRLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKA 67
           MENQVRLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKA
Sbjct: 1   MENQVRLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKA 60

Query: 68  ICDEILSQDVGREDIFITTKVWGNDHHDPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWA 127
           ICDEILSQ+V REDIFITTKVWGNDHHDPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWA
Sbjct: 61  ICDEILSQNVEREDIFITTKVWGNDHHDPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWA 120

Query: 128 CYPVPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQ 187
           CYPVPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLE ASIPPAVNQ
Sbjct: 121 CYPVPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEIASIPPAVNQ 180

Query: 188 VEMHPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQ 247
           VEMHPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSI+LKH+ATPAQ
Sbjct: 181 VEMHPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSISLKHSATPAQ 240

Query: 248 VALQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDT 307
           VALQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDT
Sbjct: 241 VALQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDT 300

Query: 308 TSPFKIIQDLWDGEI 322
           TSP+KIIQDLWDGEI
Sbjct: 301 TSPYKIIQDLWDGEI 315

BLAST of Cp4.1LG14g05530 vs. NCBI nr
Match: XP_038874693.1 (NADPH-dependent aldo-keto reductase, chloroplastic [Benincasa hispida])

HSP 1 Score: 596 bits (1536), Expect = 4.53e-214
Identity = 281/313 (89.78%), Postives = 298/313 (95.21%), Query Frame = 0

Query: 10  NQVRLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKAIC 69
           NQV LNCGVNLPI GFGTF+YQ  + AIESAVHMAIKMG+RHFDTATIYGSEGA+GKAI 
Sbjct: 4   NQVSLNCGVNLPILGFGTFAYQIDANAIESAVHMAIKMGYRHFDTATIYGSEGALGKAIN 63

Query: 70  DEILSQDVGREDIFITTKVWGNDHHDPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWACY 129
           DE L++ VGREDIF+T+KVWGNDHHDPVSALK++LR MG+EYIDMYLVHWP KLKPWACY
Sbjct: 64  DEFLNKTVGREDIFLTSKVWGNDHHDPVSALKRTLRIMGVEYIDMYLVHWPFKLKPWACY 123

Query: 130 PVPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQVE 189
           PVPKESDFEEALDLESTWKGMEKC ELGLCRCIGVSNFSTKKLESLLEFA+IPPAVNQVE
Sbjct: 124 PVPKESDFEEALDLESTWKGMEKCIELGLCRCIGVSNFSTKKLESLLEFATIPPAVNQVE 183

Query: 190 MHPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQVA 249
           MHPMWRQ KLREFCG+HKIHVSAYSPLGGPGNAWGSTAVIESP+IKSIALKHNATPAQVA
Sbjct: 184 MHPMWRQNKLREFCGDHKIHVSAYSPLGGPGNAWGSTAVIESPIIKSIALKHNATPAQVA 243

Query: 250 LQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDTTS 309
           LQWGL KGSSVIVKSFNE R+KENFGALDLKLDDQDCLEIEKLEERKI+RGEYFVNDTTS
Sbjct: 244 LQWGLWKGSSVIVKSFNEKRLKENFGALDLKLDDQDCLEIEKLEERKILRGEYFVNDTTS 303

Query: 310 PFKIIQDLWDGEI 322
           P+K IQDLWDGEI
Sbjct: 304 PYKTIQDLWDGEI 316

BLAST of Cp4.1LG14g05530 vs. ExPASy TrEMBL
Match: A0A6J1J520 (NADPH-dependent aldo-keto reductase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111483489 PE=4 SV=1)

HSP 1 Score: 649 bits (1675), Expect = 1.37e-235
Identity = 308/315 (97.78%), Postives = 313/315 (99.37%), Query Frame = 0

Query: 8   MENQVRLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKA 67
           MENQVRLNCG+NLP FGFGTFSYQAHSK IESAVHMAIKMGHRHFDTATIYGSEGAVGKA
Sbjct: 1   MENQVRLNCGLNLPHFGFGTFSYQAHSKTIESAVHMAIKMGHRHFDTATIYGSEGAVGKA 60

Query: 68  ICDEILSQDVGREDIFITTKVWGNDHHDPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWA 127
           ICDEIL+Q+VGREDIFITTKVWGNDHHDPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWA
Sbjct: 61  ICDEILNQNVGREDIFITTKVWGNDHHDPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWA 120

Query: 128 CYPVPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQ 187
           CYPVPKESDFEEALD+ESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQ
Sbjct: 121 CYPVPKESDFEEALDIESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQ 180

Query: 188 VEMHPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQ 247
           VEMHPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQ
Sbjct: 181 VEMHPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQ 240

Query: 248 VALQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDT 307
           VALQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDT
Sbjct: 241 VALQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDT 300

Query: 308 TSPFKIIQDLWDGEI 322
           TSP+KIIQDLWDGEI
Sbjct: 301 TSPYKIIQDLWDGEI 315

BLAST of Cp4.1LG14g05530 vs. ExPASy TrEMBL
Match: A0A6J1E5X8 (NADPH-dependent aldo-keto reductase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111430879 PE=4 SV=1)

HSP 1 Score: 648 bits (1672), Expect = 3.93e-235
Identity = 309/315 (98.10%), Postives = 313/315 (99.37%), Query Frame = 0

Query: 8   MENQVRLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKA 67
           MENQVRLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKA
Sbjct: 1   MENQVRLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKA 60

Query: 68  ICDEILSQDVGREDIFITTKVWGNDHHDPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWA 127
           ICDEILSQ+V REDIFITTKVWGNDHHDPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWA
Sbjct: 61  ICDEILSQNVEREDIFITTKVWGNDHHDPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWA 120

Query: 128 CYPVPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQ 187
           CYPVPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLE ASIPPAVNQ
Sbjct: 121 CYPVPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEIASIPPAVNQ 180

Query: 188 VEMHPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQ 247
           VEMHPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSI+LKH+ATPAQ
Sbjct: 181 VEMHPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSISLKHSATPAQ 240

Query: 248 VALQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDT 307
           VALQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDT
Sbjct: 241 VALQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDT 300

Query: 308 TSPFKIIQDLWDGEI 322
           TSP+KIIQDLWDGEI
Sbjct: 301 TSPYKIIQDLWDGEI 315

BLAST of Cp4.1LG14g05530 vs. ExPASy TrEMBL
Match: A0A1S3BXH5 (aldo-keto reductase family 4 member C9-like OS=Cucumis melo OX=3656 GN=LOC103494487 PE=4 SV=1)

HSP 1 Score: 575 bits (1483), Expect = 4.56e-206
Identity = 270/315 (85.71%), Postives = 297/315 (94.29%), Query Frame = 0

Query: 10  NQV-RLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKAI 69
           NQV  LNCGV LPIFGFGTF+YQ  + AIESAVHMAIKMG+RHFDTATIYGSEGAVGKA+
Sbjct: 17  NQVINLNCGVKLPIFGFGTFAYQIDTNAIESAVHMAIKMGYRHFDTATIYGSEGAVGKAL 76

Query: 70  CDEILS-QDVGREDIFITTKVWGNDHHDPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWA 129
            DE L+ + +GREDIF+T+KVWG+DHHDPVSALK++LRNMG+EY+DMYLVHWP KLKPWA
Sbjct: 77  NDEFLNYRTLGREDIFLTSKVWGSDHHDPVSALKRTLRNMGMEYLDMYLVHWPFKLKPWA 136

Query: 130 CYPVPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQ 189
           CYPVPKESDFEE LDLESTWKGMEKC ELGLCRCIGVSNFSTKKL+SLL+FA+IPPAVNQ
Sbjct: 137 CYPVPKESDFEETLDLESTWKGMEKCLELGLCRCIGVSNFSTKKLDSLLQFATIPPAVNQ 196

Query: 190 VEMHPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQ 249
           VEMHPMWRQ+KLRE+C EHK+HVSAYSPLGGPGNAWGSTAVIESP+IKSIALKHNATPAQ
Sbjct: 197 VEMHPMWRQKKLREYCEEHKVHVSAYSPLGGPGNAWGSTAVIESPIIKSIALKHNATPAQ 256

Query: 250 VALQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDT 309
           VALQWGL KGSSVIVKSFNE R++ENFGALDLKLD +DCLEIEKLEE+KI+RGEYFVNDT
Sbjct: 257 VALQWGLLKGSSVIVKSFNEDRLRENFGALDLKLDGEDCLEIEKLEEKKILRGEYFVNDT 316

Query: 310 TSPFKIIQDLWDGEI 322
           TSP+K IQDLWDGEI
Sbjct: 317 TSPYKTIQDLWDGEI 331

BLAST of Cp4.1LG14g05530 vs. ExPASy TrEMBL
Match: A0A6J1CHY6 (NADPH-dependent aldo-keto reductase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111011667 PE=4 SV=1)

HSP 1 Score: 568 bits (1465), Expect = 1.45e-203
Identity = 266/315 (84.44%), Postives = 288/315 (91.43%), Query Frame = 0

Query: 8   MENQVRLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKA 67
           M NQVRLNCGVNLPI G GTFSYQ   KAIESAVHMAI MG+RHFDTATIYGSE AVG+A
Sbjct: 2   MRNQVRLNCGVNLPIIGLGTFSYQIDPKAIESAVHMAINMGYRHFDTATIYGSEAAVGRA 61

Query: 68  ICDEILSQDVGREDIFITTKVWGNDHHDPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWA 127
           I   IL + V REDIF+T+KVWG+DHHDPVSAL Q+LR +G+EYIDMYLVHWPLKLKPWA
Sbjct: 62  ISAAILDKTVAREDIFLTSKVWGSDHHDPVSALNQTLRKLGVEYIDMYLVHWPLKLKPWA 121

Query: 128 CYPVPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQ 187
           CYPVPKE DFE+ LDLE TW GMEKC +LGLCRCIG+SNFST K+ES+LEFASIPPAVNQ
Sbjct: 122 CYPVPKEGDFEKVLDLERTWAGMEKCLDLGLCRCIGLSNFSTNKVESILEFASIPPAVNQ 181

Query: 188 VEMHPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQ 247
           VEMHPMWRQRK+REFCGEHKIHVSAYSPLGGPGNAWGSTAVI+SP+I+SIALKH ATPAQ
Sbjct: 182 VEMHPMWRQRKIREFCGEHKIHVSAYSPLGGPGNAWGSTAVIQSPVIQSIALKHKATPAQ 241

Query: 248 VALQWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDT 307
           VALQWGL KGSSVIVKSFNEHR+KEN GALDLKLDD+D LEIEKLEERKI+RGEYFVNDT
Sbjct: 242 VALQWGLWKGSSVIVKSFNEHRLKENIGALDLKLDDEDYLEIEKLEERKILRGEYFVNDT 301

Query: 308 TSPFKIIQDLWDGEI 322
           TSP+K IQDLWDGEI
Sbjct: 302 TSPYKTIQDLWDGEI 316

BLAST of Cp4.1LG14g05530 vs. ExPASy TrEMBL
Match: A0A5A7TMD4 (Aldo-keto reductase family 4 member C9-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003980 PE=4 SV=1)

HSP 1 Score: 523 bits (1347), Expect = 1.03e-182
Identity = 241/281 (85.77%), Postives = 268/281 (95.37%), Query Frame = 0

Query: 43  MAIKMGHRHFDTATIYGSEGAVGKAICDEILS-QDVGREDIFITTKVWGNDHHDPVSALK 102
           MAIKMG+RHFDTATIYGSEGAVGKA+ DE L+ + +GREDIF+T+KVWG+DHHDPVSALK
Sbjct: 1   MAIKMGYRHFDTATIYGSEGAVGKALNDEFLNYRTLGREDIFLTSKVWGSDHHDPVSALK 60

Query: 103 QSLRNMGLEYIDMYLVHWPLKLKPWACYPVPKESDFEEALDLESTWKGMEKCQELGLCRC 162
           ++LRNMG+EY+DMYLVHWP KLKPWACYPVPKESDFEE LDLESTWKGMEKC ELGLCRC
Sbjct: 61  RTLRNMGMEYLDMYLVHWPFKLKPWACYPVPKESDFEETLDLESTWKGMEKCLELGLCRC 120

Query: 163 IGVSNFSTKKLESLLEFASIPPAVNQVEMHPMWRQRKLREFCGEHKIHVSAYSPLGGPGN 222
           IGVSNFSTKKL+SLL+FA+IPPAVNQVEMHPMWRQ+KLRE+C EHK+HVSAYSPLGGPGN
Sbjct: 121 IGVSNFSTKKLDSLLQFATIPPAVNQVEMHPMWRQKKLREYCEEHKVHVSAYSPLGGPGN 180

Query: 223 AWGSTAVIESPLIKSIALKHNATPAQVALQWGLSKGSSVIVKSFNEHRMKENFGALDLKL 282
           AWGSTAVIESP+IKSIALKHNATPAQVALQWGL KGSSVI KSFNE R++ENFGALDLKL
Sbjct: 181 AWGSTAVIESPIIKSIALKHNATPAQVALQWGLLKGSSVIAKSFNEDRLRENFGALDLKL 240

Query: 283 DDQDCLEIEKLEERKIMRGEYFVNDTTSPFKIIQDLWDGEI 322
           DD+DCLEIEKLEE+KI+RGEYFVNDTTSP+K IQDLWD ++
Sbjct: 241 DDEDCLEIEKLEEKKILRGEYFVNDTTSPYKTIQDLWDAKL 281

BLAST of Cp4.1LG14g05530 vs. TAIR 10
Match: AT5G62420.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 436.8 bits (1122), Expect = 1.5e-122
Identity = 202/310 (65.16%), Postives = 248/310 (80.00%), Query Frame = 0

Query: 13  RLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKAICDEI 72
           RL CG  +P+ G GT+  Q   ++  SAVH AIK+G+RHFDTA IYGSE A+G A+   I
Sbjct: 7   RLRCGETIPLLGMGTYCPQKDRESTISAVHQAIKIGYRHFDTAKIYGSEEALGTALGQAI 66

Query: 73  LSQDVGREDIFITTKVWGNDHHDPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWACYPVP 132
               V R+D+F+T+K+W +DHHDP+SAL Q+L+ MGL+Y+D YLVHWP+KLKP    P+P
Sbjct: 67  SYGTVQRDDLFVTSKLWSSDHHDPISALIQTLKTMGLDYLDNYLVHWPIKLKPGVSEPIP 126

Query: 133 KESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQVEMHP 192
           KE +FE+ L +E TW+GME+C E+GLCR IGVSNFS+KK+  LL+FAS+ P+VNQVEMHP
Sbjct: 127 KEDEFEKDLGIEETWQGMERCLEMGLCRSIGVSNFSSKKIFDLLDFASVSPSVNQVEMHP 186

Query: 193 MWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQVALQW 252
           +WRQRKLR+ C E+ IHVS YSPLGGPGN WGSTAVIE P+IKSIALKHNATPAQVAL+W
Sbjct: 187 LWRQRKLRKVCEENNIHVSGYSPLGGPGNCWGSTAVIEHPIIKSIALKHNATPAQVALRW 246

Query: 253 GLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDTTSPFK 312
           G+SKG+SVIVKSFN  RM EN  AL++KLDDQD   I+ LEE KIMRG++ VN TTSP+K
Sbjct: 247 GMSKGASVIVKSFNGARMIENKRALEIKLDDQDLSLIDHLEEWKIMRGDFLVNQTTSPYK 306

Query: 313 IIQDLWDGEI 323
            IQ LWD EI
Sbjct: 307 SIQQLWDNEI 316

BLAST of Cp4.1LG14g05530 vs. TAIR 10
Match: AT2G37770.2 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 295.8 bits (756), Expect = 4.2e-80
Identity = 143/312 (45.83%), Postives = 200/312 (64.10%), Query Frame = 0

Query: 13  RLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKAICDEI 72
           +LN G   P  G GT  +QA    +  AV  A+K+G+RH D A IYG+E  +G  +    
Sbjct: 9   KLNTGAKFPSVGLGT--WQASPGLVGDAVAAAVKIGYRHIDCAQIYGNEKEIGAVLKKLF 68

Query: 73  LSQDVGREDIFITTKVWGNDH--HDPVSALKQSLRNMGLEYIDMYLVHWPLKLKPWACYP 132
             + V RED+FIT+K+W  DH   D   AL ++L+++ LEY+D+YL+HWP ++K  +   
Sbjct: 69  EDRVVKREDLFITSKLWCTDHDPQDVPEALNRTLKDLQLEYVDLYLIHWPARIKKGSVGI 128

Query: 133 VPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQVEM 192
            P+       +D+ STWK ME   + G  R IGVSNFSTKKL  LLE A +PPAVNQVE 
Sbjct: 129 KPENL---LPVDIPSTWKAMEALYDSGKARAIGVSNFSTKKLADLLELARVPPAVNQVEC 188

Query: 193 HPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQVAL 252
           HP WRQ KL+EFC    +H+SAYSPLG PG  W  + V+++P++  +A K   +PAQVAL
Sbjct: 189 HPSWRQTKLQEFCKSKGVHLSAYSPLGSPGTTWLKSDVLKNPILNMVAEKLGKSPAQVAL 248

Query: 253 QWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDTTSP 312
           +WGL  G SV+ KS NE R+KENF   D  + D    +  ++E+ +++ G + V++T SP
Sbjct: 249 RWGLQMGHSVLPKSTNEGRIKENFNVFDWSIPDYMFAKFAEIEQARLVTGSFLVHETLSP 308

Query: 313 FKIIQDLWDGEI 323
           +K I++LWDGEI
Sbjct: 309 YKSIEELWDGEI 315

BLAST of Cp4.1LG14g05530 vs. TAIR 10
Match: AT3G53880.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 277.3 bits (708), Expect = 1.5e-74
Identity = 137/312 (43.91%), Postives = 198/312 (63.46%), Query Frame = 0

Query: 13  RLNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKAICDEI 72
           +LN G  +P  G GT  +QA    +  AV  A+K+G++H D A+ YG+E  +GK +    
Sbjct: 9   QLNTGAKIPSVGLGT--WQAAPGVVGDAVAAAVKIGYQHIDCASRYGNEIEIGKVLKKLF 68

Query: 73  LSQDVGREDIFITTKVWGNDHHDP--VSALKQSLRNMGLEYIDMYLVHWPLKLKPWACYP 132
               V RE +FIT+K+W  D   P    AL ++L+++ L+Y+D+YL+HWP++LK      
Sbjct: 69  DDGVVKREKLFITSKIWLTDLDPPDVQDALNRTLQDLQLDYVDLYLMHWPVRLKKGT--- 128

Query: 133 VPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQVEM 192
           V  + +    +D+ STWK ME   + G  R IGVSNFSTKKL  L+E A +PPAVNQVE 
Sbjct: 129 VDFKPENIMPIDIPSTWKAMEALVDSGKARAIGVSNFSTKKLSDLVEAARVPPAVNQVEC 188

Query: 193 HPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQVAL 252
           HP W+Q KL EFC    IH+S YSPLG PG  W    V++SP+I+ IA +   +PAQ AL
Sbjct: 189 HPSWQQHKLHEFCKSKGIHLSGYSPLGSPGTTWVKADVLKSPVIEMIAKEIGKSPAQTAL 248

Query: 253 QWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDTTSP 312
           +WGL  G S++ KS NE R++ENF  L   +  +   +  K+E+ ++++G  FV++T SP
Sbjct: 249 RWGLQMGHSILPKSTNEGRIRENFDVLGWSIPKEMFDKFSKIEQARLVQGTSFVHETLSP 308

Query: 313 FKIIQDLWDGEI 323
           +K +++LWDGEI
Sbjct: 309 YKTLEELWDGEI 315

BLAST of Cp4.1LG14g05530 vs. TAIR 10
Match: AT2G37790.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 267.3 bits (682), Expect = 1.6e-71
Identity = 134/312 (42.95%), Postives = 198/312 (63.46%), Query Frame = 0

Query: 14  LNCGVNLPIFGFGTFSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKAICDEIL 73
           LN G  +P  G GT  +QA    + +AV  A+K+G+RH D A IYG+E  +G  +     
Sbjct: 10  LNTGAKIPSVGLGT--WQADPGLVGNAVDAAVKIGYRHIDCAQIYGNEKEIGLVLKKLFD 69

Query: 74  SQDVGREDIFITTKVWGNDHHDP---VSALKQSLRNMGLEYIDMYLVHWPLKLKPWACYP 133
              V RE++FIT+K+W   +HDP     AL ++L+++ L+Y+D+YL+HWP+ LK  +   
Sbjct: 70  GGVVKREEMFITSKLWCT-YHDPQEVPEALNRTLQDLQLDYVDLYLIHWPVSLKKGSTGF 129

Query: 134 VPKESDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQVEM 193
            P+        D+ STWK ME   + G  R IGVSNFS+KKL  LL  A +PPAVNQVE 
Sbjct: 130 KPENI---LPTDIPSTWKAMESLFDSGKARAIGVSNFSSKKLADLLVVARVPPAVNQVEC 189

Query: 194 HPMWRQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQVAL 253
           HP W+Q  LR+FC    +H+S YSPLG PG  W ++ V+++P++  +A K   TPAQVAL
Sbjct: 190 HPSWQQNVLRDFCKSKGVHLSGYSPLGSPGTTWLTSDVLKNPILGGVAEKLGKTPAQVAL 249

Query: 254 QWGLSKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIEKLEERKIMRGEYFVNDTTSP 313
           +WGL  G SV+ KS +E R+K+NF   +  + +    +  ++ + +++RG  FV++ TSP
Sbjct: 250 RWGLQMGQSVLPKSTHEDRIKQNFDVFNWSIPEDMLSKFSEIGQGRLVRGMSFVHE-TSP 309

Query: 314 FKIIQDLWDGEI 323
           +K +++LWDGEI
Sbjct: 310 YKSLEELWDGEI 314

BLAST of Cp4.1LG14g05530 vs. TAIR 10
Match: AT1G59960.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 258.8 bits (660), Expect = 5.6e-69
Identity = 129/309 (41.75%), Postives = 194/309 (62.78%), Query Frame = 0

Query: 19  NLPIFGFGT-FSYQAHSKAIESAVHMAIKMGHRHFDTATIYGSEGAVGKAICDEI-LSQD 78
           ++P+ GFGT  S       ++  V  AIK+G+RHFDT+  Y +E  +G+A+ + + L   
Sbjct: 20  SMPVLGFGTAASPLPEPTMLKETVIEAIKLGYRHFDTSPRYQTEEPIGEALAEAVSLGLV 79

Query: 79  VGREDIFITTKVWGNDHHD--PVSALKQSLRNMGLEYIDMYLVHWPLKLKPWACYPVPKE 138
             R + F+TTK+W  D H    V A+K+SL+N+ L+Y+D+Y++HWP+  KP   Y  P +
Sbjct: 80  RSRSEFFVTTKLWCADAHGGLVVPAIKRSLKNLKLDYLDLYIIHWPVSSKP-GKYKFPID 139

Query: 139 SDFEEALDLESTWKGMEKCQELGLCRCIGVSNFSTKKLESLLEFASIPPAVNQVEMHPMW 198
            D    +D E  W  ME+CQ LGL +CIGVSNFS KKL+ +L  A+IPP+VNQVEM P+W
Sbjct: 140 EDDFMPMDFEVVWSEMEECQRLGLAKCIGVSNFSCKKLQHILSIATIPPSVNQVEMSPIW 199

Query: 199 RQRKLREFCGEHKIHVSAYSPLGGPGNAWGSTAVIESPLIKSIALKHNATPAQVALQWGL 258
           +QRKLRE C  + I V+AYS LG  G  WG+  ++ES ++K IA     T AQV+++W  
Sbjct: 200 QQRKLRELCRSNDIVVTAYSVLGSRGAFWGTPKIMESDVLKEIAEAKEKTVAQVSMRWAY 259

Query: 259 SKGSSVIVKSFNEHRMKENFGALDLKLDDQDCLEIE-KLEERKIMRGEYFVNDTTSPFKI 318
            +G S++VKSF + R++EN    D  L + +   I  ++ + + + GE + +    P K 
Sbjct: 260 EQGVSMVVKSFTKERLEENLKIFDWSLTEDETQRISTEIPQFRNVHGEVYTS-KKGPIKS 319

Query: 319 IQDLWDGEI 323
           + ++WDGEI
Sbjct: 320 VAEMWDGEI 326

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0PGJ65.8e-7945.83NADPH-dependent aldo-keto reductase, chloroplastic OS=Arabidopsis thaliana OX=37... [more]
Q9SQ641.4e-7744.94Non-functional NADPH-dependent codeinone reductase 2 OS=Papaver somniferum OX=34... [more]
B4F9A45.7e-7445.05Deoxymugineic acid synthase 1 OS=Zea mays OX=4577 GN=DMAS1 PE=1 SV=1[more]
Q10PE77.4e-7445.63Deoxymugineic acid synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DMAS1 ... [more]
Q9M3382.2e-7343.91Aldo-keto reductase family 4 member C11 OS=Arabidopsis thaliana OX=3702 GN=AKR4C... [more]
Match NameE-valueIdentityDescription
XP_023551746.12.16e-239100.00NADPH-dependent aldo-keto reductase, chloroplastic [Cucurbita pepo subsp. pepo][more]
KAG7014834.14.89e-23698.41NADPH-dependent aldo-keto reductase, chloroplastic, partial [Cucurbita argyrospe... [more]
XP_022985497.12.83e-23597.78NADPH-dependent aldo-keto reductase, chloroplastic [Cucurbita maxima][more]
XP_022923116.18.11e-23598.10NADPH-dependent aldo-keto reductase, chloroplastic [Cucurbita moschata][more]
XP_038874693.14.53e-21489.78NADPH-dependent aldo-keto reductase, chloroplastic [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1J5201.37e-23597.78NADPH-dependent aldo-keto reductase, chloroplastic OS=Cucurbita maxima OX=3661 G... [more]
A0A6J1E5X83.93e-23598.10NADPH-dependent aldo-keto reductase, chloroplastic OS=Cucurbita moschata OX=3662... [more]
A0A1S3BXH54.56e-20685.71aldo-keto reductase family 4 member C9-like OS=Cucumis melo OX=3656 GN=LOC103494... [more]
A0A6J1CHY61.45e-20384.44NADPH-dependent aldo-keto reductase, chloroplastic OS=Momordica charantia OX=367... [more]
A0A5A7TMD41.03e-18285.77Aldo-keto reductase family 4 member C9-like OS=Cucumis melo var. makuwa OX=11946... [more]
Match NameE-valueIdentityDescription
AT5G62420.11.5e-12265.16NAD(P)-linked oxidoreductase superfamily protein [more]
AT2G37770.24.2e-8045.83NAD(P)-linked oxidoreductase superfamily protein [more]
AT3G53880.11.5e-7443.91NAD(P)-linked oxidoreductase superfamily protein [more]
AT2G37790.11.6e-7142.95NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G59960.15.6e-6941.75NAD(P)-linked oxidoreductase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020471Aldo-keto reductasePRINTSPR00069ALDKETRDTASEcoord: 44..68
score: 52.47
coord: 102..120
score: 49.72
coord: 150..167
score: 53.59
coord: 228..252
score: 40.24
coord: 184..213
score: 46.55
IPR020471Aldo-keto reductasePIRSFPIRSF000097AKRcoord: 5..316
e-value: 1.4E-90
score: 301.3
IPR023210NADP-dependent oxidoreductase domainPFAMPF00248Aldo_ket_redcoord: 24..293
e-value: 1.9E-48
score: 165.1
IPR036812NADP-dependent oxidoreductase domain superfamilyGENE3D3.20.20.100coord: 10..322
e-value: 3.0E-103
score: 347.0
IPR036812NADP-dependent oxidoreductase domain superfamilySUPERFAMILY51430NAD(P)-linked oxidoreductasecoord: 8..300
NoneNo IPR availablePIRSRPIRSR000097-3PIRSR000097-3coord: 17..122
e-value: 1.3E-28
score: 97.5
NoneNo IPR availablePIRSRPIRSR000097-2PIRSR000097-2coord: 17..122
e-value: 1.3E-28
score: 97.5
NoneNo IPR availablePIRSRPIRSR000097-1PIRSR000097-1coord: 17..122
e-value: 1.3E-28
score: 97.5
NoneNo IPR availablePANTHERPTHR11732ALDO/KETO REDUCTASEcoord: 11..320
NoneNo IPR availablePANTHERPTHR11732:SF460SUBFAMILY NOT NAMEDcoord: 11..320
IPR018170Aldo/keto reductase, conserved sitePROSITEPS00063ALDOKETO_REDUCTASE_3coord: 261..276
IPR018170Aldo/keto reductase, conserved sitePROSITEPS00062ALDOKETO_REDUCTASE_2coord: 150..167

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG14g05530.1Cp4.1LG14g05530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005829 cytosol
molecular_function GO:0004032 alditol:NADP+ 1-oxidoreductase activity
molecular_function GO:0047834 D-threo-aldose 1-dehydrogenase activity
molecular_function GO:0016491 oxidoreductase activity