Cp4.1LG14g05220 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG14g05220
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptiondicarboxylate transporter 2.1, chloroplastic-like
LocationCp4.1LG14: 1139196 .. 1142744 (+)
RNA-Seq ExpressionCp4.1LG14g05220
SyntenyCp4.1LG14g05220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATATATTGCTCTGTTTGGGTAATATATTACAAATACACAATTAAAAGAAAAATAGGTGGTGCAATAAAAAAGTGACACGTAAGAGCGGCGCATAATAGTGTATGTGGTTCACTACATCCGGAAAGAAAGAGCGGCATCCAAATTCACTCTCCGCCATAGGCATTAGCAGCCAGCAATCACCACCAAGCCCCACTCACTCACTTCGTTCAATCCGCTCTGCTTCTGCGTCTGCTTCAAAACGCCATAAACTTCGCCCTTCTCTCTGATCTCTCGATTCTCCTGGTCCTCCGCCGTCGTTCATTGTCTCTTACCGATCTTCATCACAATGGAGAGTTTTGCCCTCCAATCTGTCTCCACCACCGCTTCCTTGCCCCGCCATTCTCCCATTTCTCGAATTTCTCTCCTCCACCGCCGGAAAACCATTCCCCATTCCCAATCCTTTCCCACTTCCCCTTGTTTATATCGCTCGGTTTCGCTATTTCAATCAGCCCCACCGATCCCCCAATCCCCCCTTTTCATTTCCCGATCTTCTTCCCTTTTCAATCCGCTTTTCAATCCCAAATTGCGTCGAACCCATCAATTACGAACCAGTGCCTCTCAATCACCGGAGAAGCAGCCACTGCCGCCGACCCCACTTCCTCAACAAGGTGCTAAACCAATCCCTTTTCTGATTTCCATCGCAATTGGTTTAATTGTTCGTTTTGCCGTCCCGAAACCGGTGGAGGTTTCTGCCCAAGCGTGGCAGTTGCTCTCGATTTTCTTGTCCACGATTGCTGGACTCGTTTTGAGTCCCTTGCCGGTGGGTGCTTGGGCGTTTCTTGGCCTCACTATGACTGTTGTTACTAAGACTTTGACGTTTGCTTCTGCTTTTAATGCTTTCACTAGTGAGGTGATTTGGTTGATTGTTATTTCATTCTTTTTCGCTCGTGGGTTTGTCAAAACTGGGTTGGGGGATCGAATTGCGACGTATTTTGTTAAATGGATGGGGAAGAGTACGCTTGGTTTGTCTTACGGTTTGACGTTCAGTGAGGCATTGATTGCCCCTGCTATGCCCAGCACTACGGCCAGGGCTGGCGGTGTGTTCTTGCCCATTATCAAGTCCTTGTCGCTTGCTGCTGACAGTAAACCCAACGACCCATCGTCTAAAAAGATCGGTGCTTATCTAATTCAGTCTCAGTTTCAGGTATGTTGCTATATTTCTCCATTATCTTTCTTGCTCAGTTGTTTTGTTCTCCTGCCATTAACCTTACCATGGGAAATCTTTGCCTGCTGACTGACTGTTTGTTCTATGGCTTCAATTTATCCAATCATTCTTTGGCTTGTGTGGTTTGAAAGGAATTGGAGACTTCTCAACAACAATTACAATATCTTCATTAACTTTTTAGATACTTTCCCTTTTATTGCCCCCTCACATGGTGTAAATGTTACTATGCTCCTTACAAAAGCTATAGTTTAAACACCCTTTTCTCCAATTAGATGAACTTTTTGTGATCTCCATTGTTTTGGGGCTTGGTGTCATAATCGTTTTCTCGGGAGAACAATTGTGCGGTACTTGTTTTAACCCAACGAACAAGTTAGCTGTGAGAGATTCGGACTTTGCAGACAACGTCAGCATATACTCGAGCCTCAAGTCACGTTTGAGTGAAATGTTTTGGAAAACGTGAACTAGGAAAGACGCTGAAAACAATGTTGTAAGCTAAAAGCGATTAGGCAAGAACAACGGTTTTGATAGCATATAGTCTGGGTAGGAAGAACTGACGACTAATCATATCCACTATAGTACATTTTGGGGCGTTTTAGCTGCTGTTATCGAGTTACATCTATGACGATCTTCTTCTTGAATGTTTGCTACCGTTATCAGTTTGCATAACTTGAATTCTGTCTATAAATATTTGTTGATATGTCAAACAAGGAAAACATTTTGATCTCGAGCATTTTTGGAGCCCATGTGCTGATAAGTAATACGTTCTGTAACTCGTTATTGCCTGCTTATTTGGTGACAGAGTGCTGGTAACTCTAGTGCCCTTTTCCTAACTGCTGCTGCTCAAAACTTATTGTGCCTTAAACTGGCTGAAGAACTTGGTGTCCAAATATCTAGCCCCTGGCTTACTTGGGCCAAAATTGCTAGTGTGCCAGCCATCATTTGTCTCTTAACAACTCCAGCAATCTTATACAAGCTGTTTCCTCCCGAAACCAAGAACACACCCGACGCCCCAGCCATGGCGACGAAAAAGTTGGAGGCTATGGGTCCTGTCACCAAAAATGAATGGGTCATGGTCGGTACCATGCTTATTGCAGTCTCGTTATGGATAGCTGGGTATGTAGTATTACTTGTGCATTATCATATAAATCTTTTGCTTAAGTGATGTGATCGTGTTTAGAGAAGAATGTGTAACTTTCTCGGCTGGCGTTTTCGTTACTTCATCATGCCCGTCTTCTACTGGTTAAAGCATTAGCATAATCTTAGGACCTGAAAGTTGCTGCTACTTCTTATGGATGTTAATAATGGTCTCTTAGTTGTTAACTGCTCCCTCTGGGTTTGGAACTAAGCTTTTTCTCGTTTACTTTCAGAGAGGCCCTTCAAATTCCTAGTGTTGTAGCTGCAATGATTGGTTTATCAACACTCCTTACACTCGGAGTCCTAAACTGGGAAGACTGCTTAGCCGAAAAGTCGGCCTGGGACACGTTGGCTTGGTTTGCTGTCCTCGTGGGCATGGCGGGCCAATTGACGAGTCTCGGTCTGGTTGGCTGGATGTCCAATTGTGTGGCCAGTTTGTTGACATCTCTCTCTCTCAGCTGGCCTGCAGCTTTTGCTATTCTTCAGCTATCTTACTTCTTTGTTCACTACCTCTTCGCAAGCCAAACCGGTCACGTCGGAGCTTTATACTCGGCATTCCTTGCGATGCACTTGGCTGCCAAGGTGCCCGGGGTATTAGCAGCTCTCGCCTTAGCTTACAACACGAATCTCTTTGGCGCTCTGACTCATTACAGCAGTGGTCAAGCTGCTGTATACTATGGAGGTATGTATGCTCATCAAACATCTTTGAATTCAGACAATGGATATTAACTGACTTACTTGTTTGTTCATTGCAACAGCGGGTTATGTCGACCTACCAGACGTATTCAAAATCGGGTTCGTGATGGCGCTCGTAAACGGTATTATCTGGATTGTGGCTGGAGGTATTTGGTGGAAGGTTCTTGGTCTATACTGAACATGAAATCTTGAATCCAAAGAGTCTTTTGTTTTGTACATGCTCTGCTCAGTCTTTGGGTACCCACCGGAAGTCCTGACCCAATTGTTTCTCCTTCTTCTCTCTGGCTCTACTGATGGTGGCATTGATAAAGATTTAGATTAAATTTTGCCTTGTCTTATAACCTTTTCAAACAGTGAAAAGGGAAATTGATGATGAATTAAATCCATAATTTGGTGGAAAACAATCTGAAAAGCTGAGTTTCTGAATCTTCTTTGTGTTGTGTTCTTGATTTCTATGTTTCGATTCACTCGGTTTTGGTTTCCAGAAATTAATTTGTTTCTTCTTTATAAACGAT

mRNA sequence

ATATATTGCTCTGTTTGGGTAATATATTACAAATACACAATTAAAAGAAAAATAGGTGGTGCAATAAAAAAGTGACACGTAAGAGCGGCGCATAATAGTGTATGTGGTTCACTACATCCGGAAAGAAAGAGCGGCATCCAAATTCACTCTCCGCCATAGGCATTAGCAGCCAGCAATCACCACCAAGCCCCACTCACTCACTTCGTTCAATCCGCTCTGCTTCTGCGTCTGCTTCAAAACGCCATAAACTTCGCCCTTCTCTCTGATCTCTCGATTCTCCTGGTCCTCCGCCGTCGTTCATTGTCTCTTACCGATCTTCATCACAATGGAGAGTTTTGCCCTCCAATCTGTCTCCACCACCGCTTCCTTGCCCCGCCATTCTCCCATTTCTCGAATTTCTCTCCTCCACCGCCGGAAAACCATTCCCCATTCCCAATCCTTTCCCACTTCCCCTTGTTTATATCGCTCGGTTTCGCTATTTCAATCAGCCCCACCGATCCCCCAATCCCCCCTTTTCATTTCCCGATCTTCTTCCCTTTTCAATCCGCTTTTCAATCCCAAATTGCGTCGAACCCATCAATTACGAACCAGTGCCTCTCAATCACCGGAGAAGCAGCCACTGCCGCCGACCCCACTTCCTCAACAAGGTGCTAAACCAATCCCTTTTCTGATTTCCATCGCAATTGGTTTAATTGTTCGTTTTGCCGTCCCGAAACCGGTGGAGGTTTCTGCCCAAGCGTGGCAGTTGCTCTCGATTTTCTTGTCCACGATTGCTGGACTCGTTTTGAGTCCCTTGCCGGTGGGTGCTTGGGCGTTTCTTGGCCTCACTATGACTGTTGTTACTAAGACTTTGACGTTTGCTTCTGCTTTTAATGCTTTCACTAGTGAGGTGATTTGGTTGATTGTTATTTCATTCTTTTTCGCTCGTGGGTTTGTCAAAACTGGGTTGGGGGATCGAATTGCGACGTATTTTGTTAAATGGATGGGGAAGAGTACGCTTGGTTTGTCTTACGGTTTGACGTTCAGTGAGGCATTGATTGCCCCTGCTATGCCCAGCACTACGGCCAGGGCTGGCGGTGTGTTCTTGCCCATTATCAAGTCCTTGTCGCTTGCTGCTGACAGTAAACCCAACGACCCATCGTCTAAAAAGATCGGTGCTTATCTAATTCAGTCTCAGTTTCAGAGTGCTGGTAACTCTAGTGCCCTTTTCCTAACTGCTGCTGCTCAAAACTTATTGTGCCTTAAACTGGCTGAAGAACTTGGTGTCCAAATATCTAGCCCCTGGCTTACTTGGGCCAAAATTGCTAGTGTGCCAGCCATCATTTGTCTCTTAACAACTCCAGCAATCTTATACAAGCTGTTTCCTCCCGAAACCAAGAACACACCCGACGCCCCAGCCATGGCGACGAAAAAGTTGGAGGCTATGGGTCCTGTCACCAAAAATGAATGGGTCATGGTCGGTACCATGCTTATTGCAGTCTCGTTATGGATAGCTGGAGAGGCCCTTCAAATTCCTAGTGTTGTAGCTGCAATGATTGGTTTATCAACACTCCTTACACTCGGAGTCCTAAACTGGGAAGACTGCTTAGCCGAAAAGTCGGCCTGGGACACGTTGGCTTGGTTTGCTGTCCTCGTGGGCATGGCGGGCCAATTGACGAGTCTCGGTCTGGTTGGCTGGATGTCCAATTGTGTGGCCAGTTTGTTGACATCTCTCTCTCTCAGCTGGCCTGCAGCTTTTGCTATTCTTCAGCTATCTTACTTCTTTGTTCACTACCTCTTCGCAAGCCAAACCGGTCACGTCGGAGCTTTATACTCGGCATTCCTTGCGATGCACTTGGCTGCCAAGGTGCCCGGGGTATTAGCAGCTCTCGCCTTAGCTTACAACACGAATCTCTTTGGCGCTCTGACTCATTACAGCAGTGGTCAAGCTGCTGTATACTATGGAGCGGGTTATGTCGACCTACCAGACGTATTCAAAATCGGGTTCGTGATGGCGCTCGTAAACGGTATTATCTGGATTGTGGCTGGAGGTATTTGGTGGAAGGTTCTTGGTCTATACTGAACATGAAATCTTGAATCCAAAGAGTCTTTTGTTTTGTACATGCTCTGCTCAGTCTTTGGGTACCCACCGGAAGTCCTGACCCAATTGTTTCTCCTTCTTCTCTCTGGCTCTACTGATGGTGGCATTGATAAAGATTTAGATTAAATTTTGCCTTGTCTTATAACCTTTTCAAACAGTGAAAAGGGAAATTGATGATGAATTAAATCCATAATTTGGTGGAAAACAATCTGAAAAGCTGAGTTTCTGAATCTTCTTTGTGTTGTGTTCTTGATTTCTATGTTTCGATTCACTCGGTTTTGGTTTCCAGAAATTAATTTGTTTCTTCTTTATAAACGAT

Coding sequence (CDS)

ATGGAGAGTTTTGCCCTCCAATCTGTCTCCACCACCGCTTCCTTGCCCCGCCATTCTCCCATTTCTCGAATTTCTCTCCTCCACCGCCGGAAAACCATTCCCCATTCCCAATCCTTTCCCACTTCCCCTTGTTTATATCGCTCGGTTTCGCTATTTCAATCAGCCCCACCGATCCCCCAATCCCCCCTTTTCATTTCCCGATCTTCTTCCCTTTTCAATCCGCTTTTCAATCCCAAATTGCGTCGAACCCATCAATTACGAACCAGTGCCTCTCAATCACCGGAGAAGCAGCCACTGCCGCCGACCCCACTTCCTCAACAAGGTGCTAAACCAATCCCTTTTCTGATTTCCATCGCAATTGGTTTAATTGTTCGTTTTGCCGTCCCGAAACCGGTGGAGGTTTCTGCCCAAGCGTGGCAGTTGCTCTCGATTTTCTTGTCCACGATTGCTGGACTCGTTTTGAGTCCCTTGCCGGTGGGTGCTTGGGCGTTTCTTGGCCTCACTATGACTGTTGTTACTAAGACTTTGACGTTTGCTTCTGCTTTTAATGCTTTCACTAGTGAGGTGATTTGGTTGATTGTTATTTCATTCTTTTTCGCTCGTGGGTTTGTCAAAACTGGGTTGGGGGATCGAATTGCGACGTATTTTGTTAAATGGATGGGGAAGAGTACGCTTGGTTTGTCTTACGGTTTGACGTTCAGTGAGGCATTGATTGCCCCTGCTATGCCCAGCACTACGGCCAGGGCTGGCGGTGTGTTCTTGCCCATTATCAAGTCCTTGTCGCTTGCTGCTGACAGTAAACCCAACGACCCATCGTCTAAAAAGATCGGTGCTTATCTAATTCAGTCTCAGTTTCAGAGTGCTGGTAACTCTAGTGCCCTTTTCCTAACTGCTGCTGCTCAAAACTTATTGTGCCTTAAACTGGCTGAAGAACTTGGTGTCCAAATATCTAGCCCCTGGCTTACTTGGGCCAAAATTGCTAGTGTGCCAGCCATCATTTGTCTCTTAACAACTCCAGCAATCTTATACAAGCTGTTTCCTCCCGAAACCAAGAACACACCCGACGCCCCAGCCATGGCGACGAAAAAGTTGGAGGCTATGGGTCCTGTCACCAAAAATGAATGGGTCATGGTCGGTACCATGCTTATTGCAGTCTCGTTATGGATAGCTGGAGAGGCCCTTCAAATTCCTAGTGTTGTAGCTGCAATGATTGGTTTATCAACACTCCTTACACTCGGAGTCCTAAACTGGGAAGACTGCTTAGCCGAAAAGTCGGCCTGGGACACGTTGGCTTGGTTTGCTGTCCTCGTGGGCATGGCGGGCCAATTGACGAGTCTCGGTCTGGTTGGCTGGATGTCCAATTGTGTGGCCAGTTTGTTGACATCTCTCTCTCTCAGCTGGCCTGCAGCTTTTGCTATTCTTCAGCTATCTTACTTCTTTGTTCACTACCTCTTCGCAAGCCAAACCGGTCACGTCGGAGCTTTATACTCGGCATTCCTTGCGATGCACTTGGCTGCCAAGGTGCCCGGGGTATTAGCAGCTCTCGCCTTAGCTTACAACACGAATCTCTTTGGCGCTCTGACTCATTACAGCAGTGGTCAAGCTGCTGTATACTATGGAGCGGGTTATGTCGACCTACCAGACGTATTCAAAATCGGGTTCGTGATGGCGCTCGTAAACGGTATTATCTGGATTGTGGCTGGAGGTATTTGGTGGAAGGTTCTTGGTCTATACTGA

Protein sequence

MESFALQSVSTTASLPRHSPISRISLLHRRKTIPHSQSFPTSPCLYRSVSLFQSAPPIPQSPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAIGLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTFASAFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAPAMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAAQNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMATKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDCLAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLLTSLSLSWPAAFAILQLSYFFVHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYGAGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY
Homology
BLAST of Cp4.1LG14g05220 vs. ExPASy Swiss-Prot
Match: Q9FMF7 (Dicarboxylate transporter 2.1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DIT2-1 PE=1 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 1.3e-222
Identity = 425/586 (72.53%), Postives = 478/586 (81.57%), Query Frame = 0

Query: 1   MESFALQSVSTTA-----SLPRHSPISRISLLHRRKTIPHSQSFPTSPCLYRSVSLFQSA 60
           MESFAL S+STTA     S   H   SR+SLL R      S   P S    RS+S+    
Sbjct: 1   MESFALHSLSTTATSTLLSHHHHHHPSRLSLLRRT-----SSRSPPSTISLRSLSV---- 60

Query: 61  PPIPQSPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQ---QGAKPI 120
                          L  PL  P  R + ++  +A Q     P PP+P P    QGAK I
Sbjct: 61  -------------QPLSFPLLKPIPRFSTRI-AAAPQDNAPPPPPPSPSPSPSPQGAKLI 120

Query: 121 PFLISIAIGLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVV 180
           P ++SI++GLI+RFAVP P  V+ Q WQLLSIFLSTIAGLVLSPLPVGAWAF+GLT ++V
Sbjct: 121 PLILSISVGLILRFAVPVPEGVTPQGWQLLSIFLSTIAGLVLSPLPVGAWAFIGLTASIV 180

Query: 181 TKTLTFASAFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLT 240
           TKTL+F++AF+AFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKW+GKSTLGLSYGLT
Sbjct: 181 TKTLSFSAAFSAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWLGKSTLGLSYGLT 240

Query: 241 FSEALIAPAMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSS 300
            SEALIAPAMPSTTARAGG+FLPIIKSLSL+A SKPND SS+K+G+YLIQSQFQ AGNSS
Sbjct: 241 LSEALIAPAMPSTTARAGGIFLPIIKSLSLSAGSKPNDSSSRKLGSYLIQSQFQCAGNSS 300

Query: 301 ALFLTAAAQNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKN 360
           ALFLTAAAQNLLCLKLAEELGV IS+PW++W K AS+PAII LL TP ILYKL+PPETK+
Sbjct: 301 ALFLTAAAQNLLCLKLAEELGVVISNPWVSWFKAASLPAIISLLCTPLILYKLYPPETKD 360

Query: 361 TPDAPAMATKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTL 420
           TP+AP +A  KL+ MGPVTKNEW+MVGTML+AV+LWI GE L IPSVVAAMIGLS LL L
Sbjct: 361 TPEAPGIAATKLKQMGPVTKNEWIMVGTMLLAVTLWICGETLGIPSVVAAMIGLSILLVL 420

Query: 421 GVLNWEDCLAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLLTSLSLSWPAAFA 480
           GVLNW+DCL+EKSAWDTLAWFAVLVGMAGQLT+LG+V WMS+CVA +L SLSLSWPAAF 
Sbjct: 421 GVLNWDDCLSEKSAWDTLAWFAVLVGMAGQLTNLGVVTWMSDCVAKVLQSLSLSWPAAFG 480

Query: 481 ILQLSYFFVHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSS 540
           +LQ +YFF+HYLFASQTGHVGAL+SAFLAMH+AA VPG+LAALALAYNTNLFGALTHYSS
Sbjct: 481 LLQAAYFFIHYLFASQTGHVGALFSAFLAMHIAAGVPGILAALALAYNTNLFGALTHYSS 540

Query: 541 GQAAVYYGAGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY 579
           GQAAVYYGAGYVDLPDVFKIGFVMA +N IIW V G  WWK LGLY
Sbjct: 541 GQAAVYYGAGYVDLPDVFKIGFVMATINAIIWGVVGTFWWKFLGLY 563

BLAST of Cp4.1LG14g05220 vs. ExPASy Swiss-Prot
Match: Q8L7Z9 (Dicarboxylate transporter 2, chloroplastic OS=Spinacia oleracea OX=3562 GN=DIT2 PE=1 SV=1)

HSP 1 Score: 732.3 bits (1889), Expect = 4.4e-210
Identity = 400/583 (68.61%), Postives = 468/583 (80.27%), Query Frame = 0

Query: 1   MESFAL---QSVSTTASLPRHSPISRISL--LHRRKTIPHSQSFPTSPCLYRSVSLFQSA 60
           MES AL    S+STT +  + +   R S   LH R+  PH             +SL  S+
Sbjct: 1   MESLALLPTLSLSTTTTTSKATAFLRSSTTSLHHRR--PH-------------LSLSLSS 60

Query: 61  PPIPQSPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFL 120
            P P +  F S S S  + L +  L     L  +AS S       P P  + GAK IP +
Sbjct: 61  TPKP-TLTFSSHSHS--HSLSSKPLLALKPLNATASSSSSPATTSPPP-TKSGAKLIPLI 120

Query: 121 ISIAIGLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKT 180
           +S+++GL++RFAVPKP E++ QAWQLL+IFLST+AGLVLSPLPVGAWAFLG+T +VVTKT
Sbjct: 121 LSVSVGLLLRFAVPKPAELTPQAWQLLAIFLSTVAGLVLSPLPVGAWAFLGVTASVVTKT 180

Query: 181 LTFASAFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSE 240
           L F +AF AFT+EVIWLIVISFFFARGFVKTGLGDRIATYFVKW+GKSTLGLSYGLT SE
Sbjct: 181 LPFPTAFCAFTNEVIWLIVISFFFARGFVKTGLGDRIATYFVKWLGKSTLGLSYGLTISE 240

Query: 241 ALIAPAMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALF 300
           AL+APAMPSTTARAGG+FLPIIKSLS+++ S P   S KK+G YLI +QFQSAGNSSALF
Sbjct: 241 ALVAPAMPSTTARAGGIFLPIIKSLSISSGSLPGGESRKKLGTYLIMTQFQSAGNSSALF 300

Query: 301 LTAAAQNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPD 360
           LTAAAQNLLCLKLAEELGV+I+SPW+ W K AS+PA + LL TP ILYKL+PPE K+TP+
Sbjct: 301 LTAAAQNLLCLKLAEELGVKIASPWVFWLKAASLPAFVALLLTPLILYKLYPPELKDTPE 360

Query: 361 APAMATKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVL 420
           APA+A +KL+ MGPVTKNEWVMVGTML+AVSLW+ GE + + SVVAAM+GLS LL LGVL
Sbjct: 361 APALAAEKLKNMGPVTKNEWVMVGTMLLAVSLWVFGEKIGVSSVVAAMLGLSVLLLLGVL 420

Query: 421 NWEDCLAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLLTSLSLSWPAAFAILQ 480
           +W DCL EKSAWDTLAWFAVLVGMA QLT+LG+V WMS CVA  L +++LSWPAAF ILQ
Sbjct: 421 DWNDCLNEKSAWDTLAWFAVLVGMASQLTNLGIVSWMSGCVARSLKTMNLSWPAAFGILQ 480

Query: 481 LSYFFVHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQA 540
            +YFFVHYLFASQTGHVGALYSAFLAM++A+ VPGVLAALALAYNTNLFGALTHYSSGQA
Sbjct: 481 AAYFFVHYLFASQTGHVGALYSAFLAMNIASGVPGVLAALALAYNTNLFGALTHYSSGQA 540

Query: 541 AVYYGAGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY 579
           AVYYGAGYVDLPDVFK+GF+MA++N  IW V GG+WWK+LG+Y
Sbjct: 541 AVYYGAGYVDLPDVFKMGFIMAVINATIWTVVGGVWWKILGIY 564

BLAST of Cp4.1LG14g05220 vs. ExPASy Swiss-Prot
Match: Q9FMF8 (Dicarboxylate transporter 2.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DIT2-2 PE=2 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 1.8e-195
Identity = 369/583 (63.29%), Postives = 443/583 (75.99%), Query Frame = 0

Query: 1   MESFALQSVSTTAS-LPRHSPISRISLLHRRKTIPHSQSFPTSPCLYRSVSLFQSAPPIP 60
           MES AL+S+S +AS L  H   S+   L     +P S S  TSP L              
Sbjct: 1   MESLALRSISLSASYLSLHRSSSKSFAL-----LPPSISVHTSPTL-------------- 60

Query: 61  QSPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEK----QPLPPTPLPQQGAKPIPFL 120
                  RS S+ +P F         LR +AS  PE+    QP PP+P   QGAK IP  
Sbjct: 61  -------RSLSISSPRFT--------LRATASSLPEEQNKPQPPPPSPPQPQGAKLIPLA 120

Query: 121 ISIAIGLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKT 180
           IS++IGLIVRF +P+P +V++Q WQLLSIFL TI+GLVL PLPVGAWAF+GLT ++VTKT
Sbjct: 121 ISVSIGLIVRFLIPRPEQVTSQGWQLLSIFLFTISGLVLGPLPVGAWAFIGLTASIVTKT 180

Query: 181 LTFASAFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSE 240
           L F++AF AFT+E+IWLI ISFFFARGF+KTGLGDRIATYFVKW+GKSTLGLSYGL F E
Sbjct: 181 LPFSTAFAAFTNELIWLIAISFFFARGFIKTGLGDRIATYFVKWLGKSTLGLSYGLAFCE 240

Query: 241 ALIAPAMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALF 300
            L+   MPST ARAGGVFLP+IKSL+++A S P DPSS+K+G++LIQ+Q Q +G S A+ 
Sbjct: 241 TLMGLIMPSTMARAGGVFLPVIKSLAISAGSYPGDPSSRKLGSFLIQTQLQCSGASGAIL 300

Query: 301 LTAAAQNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPD 360
           LT+AAQNLLCLKLA E+GV IS+PW+TW K+ASVPA + LL TP I+YKL+PPE K+TP+
Sbjct: 301 LTSAAQNLLCLKLAREVGVVISNPWITWFKVASVPAFVSLLCTPLIIYKLYPPELKHTPE 360

Query: 361 APAMATKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVL 420
           APA A KKLE +GP+TKNEW+M+G M   VSLW+ GEA+ I SVV+AMIGLSTLL LGV+
Sbjct: 361 APAAAAKKLERLGPITKNEWIMLGAMAFTVSLWVFGEAIGIASVVSAMIGLSTLLLLGVI 420

Query: 421 NWEDCLAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLLTSLSLSWPAAFAILQ 480
           NW+DCL++KSAWD+L WFAVL+GMAGQLT+LG+V WMS+CVA LL SLSL+WPA+F ILQ
Sbjct: 421 NWDDCLSDKSAWDSLTWFAVLIGMAGQLTNLGVVAWMSDCVAKLLQSLSLTWPASFIILQ 480

Query: 481 LSYFFVHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQA 540
             Y  +HYLFASQTGH GALY  FLAM +AA VPGVLAAL LA+N NL GAL HYS G A
Sbjct: 481 ACYLLIHYLFASQTGHAGALYPPFLAMQIAAGVPGVLAALCLAFNNNLSGALAHYSGGPA 540

Query: 541 AVYYGAGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY 579
           A+YYGAGYVDL D+F++GFVMALV  IIW   G  WWK LGLY
Sbjct: 541 ALYYGAGYVDLRDMFRVGFVMALVQAIIWGGVGSFWWKFLGLY 549

BLAST of Cp4.1LG14g05220 vs. ExPASy Swiss-Prot
Match: Q41364 (Dicarboxylate transporter 1, chloroplastic OS=Spinacia oleracea OX=3562 GN=DIT1 PE=1 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 8.2e-132
Identity = 266/560 (47.50%), Postives = 367/560 (65.54%), Query Frame = 0

Query: 35  HSQSFPTSPCLYRSVSLFQSAPPIPQSPLFISRSSSLFNPLF--NPKLRRTHQLRTSASQ 94
           +S SF + P L + +S  Q +  +P      S+S SL +  F   P L   H+L+  ++ 
Sbjct: 14  YSLSFRSLPSL-KPLSKSQPSISLPSLRSNASKSPSLSHKHFLSPPSLLLPHKLKPISAS 73

Query: 95  SPEKQPLPPTPLPQ-------------QGAKPIPFLISIAIGLIVRFAVPKPVEVSAQAW 154
           SP   P PP P+P              QGA   P L SI  G+I+ F +P P  VS  AW
Sbjct: 74  SPTNPPPPPAPVPSPAPVSAPAQVQPWQGASIKPLLASILTGVIIWF-IPTPEGVSRNAW 133

Query: 155 QLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTFASAFNAFTSEVIWLIVISFFF 214
           QLL+IFLSTI G++  PLP+GA A +GL  +V+TKTLTF++AF+AF   + WLI ++FFF
Sbjct: 134 QLLAIFLSTIVGIITQPLPLGAVALMGLGASVLTKTLTFSAAFSAFGDPIPWLIALAFFF 193

Query: 215 ARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAPAMPSTTARAGGVFLPIIKS 274
           ARGF+KTGLG+RIA  FVK  G S+LGL Y L FSEAL+APA+PS +ARAGG+FLP++KS
Sbjct: 194 ARGFIKTGLGNRIAYQFVKLFGSSSLGLGYSLVFSEALLAPAIPSVSARAGGIFLPLVKS 253

Query: 275 LSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAAQNLLCLKLA-EELGVQISS 334
           L +A  S   D + +K+GA+L+ + FQ++  SS++FLTA A N L   L    +G  I  
Sbjct: 254 LCIACGSNVGDGTERKLGAWLMLTCFQTSVISSSMFLTAMAANPLSATLTFNTIGKAIG- 313

Query: 335 PWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMATKKLEAMGPVTKNEWVMV 394
            W+ WAK A VP ++ L+  P +LY ++PPE K++PDAP +A +KL+ MGP+TKNE +M 
Sbjct: 314 -WMDWAKAAFVPGLVSLIVVPLLLYVVYPPEIKSSPDAPRLAKEKLDKMGPMTKNESIMA 373

Query: 395 GTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDCLAEKSAWDTLAWFAVLVG 454
            T+L+ V LW+ G  L + +V AA++GLS LL  GV+ W++CLAE  AWDTL WFA L+ 
Sbjct: 374 VTLLLTVGLWVFGGKLGVDAVTAAILGLSVLLITGVVTWKECLAESVAWDTLTWFAALIA 433

Query: 455 MAGQLTSLGLVGWMSNCVASLLTSLSLSWPAAFAILQLSYFFVHYLFASQTGHVGALYSA 514
           MAG L   GL+ W S  V  ++  L LSW  +F +L L YF+ HY FAS   H+GA+++A
Sbjct: 434 MAGYLNKYGLITWFSENVVKVVGGLGLSWQMSFGVLVLLYFYSHYFFASGAAHIGAMFTA 493

Query: 515 FLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYGAGYVDLPDVFKIGFVMAL 574
           FL++  A   P  LAA+ L++ +NL G LTHY  G A V+YGA YV LP  +  GF++++
Sbjct: 494 FLSVASALGTPPFLAAIVLSFLSNLMGGLTHYGIGSAPVFYGANYVPLPQWWGYGFLISI 553

Query: 575 VNGIIWIVAGGIWWKVLGLY 579
           VN IIW+  GG+WWK +GL+
Sbjct: 554 VNLIIWLGVGGLWWKAIGLW 569

BLAST of Cp4.1LG14g05220 vs. ExPASy Swiss-Prot
Match: Q9LXV3 (Dicarboxylate transporter 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DIT1 PE=1 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 9.4e-128
Identity = 264/550 (48.00%), Postives = 357/550 (64.91%), Query Frame = 0

Query: 43  PCLYRSVSLFQSAPPIPQSPLFI--------SRSSSLFNPLFNPKLRRTHQL------RT 102
           P   RS+SL    P  P S L +        SR S L +PL     RR+  L        
Sbjct: 15  PLKSRSLSL----PRPPSSSLNLTKPLRSLDSRFSLLKSPLPVSLRRRSSTLVKASSTVA 74

Query: 103 SASQSPEKQPLPPTPLPQQGAKPIPFLISIAIGLIVRFAVPKPVEVSAQAWQLLSIFLST 162
           SAS SP   PL P P+P QGA   P L SIA GLI+ F VP P  V+  AWQLL+IFL+T
Sbjct: 75  SASSSP-TPPLVPAPVPWQGAAIKPLLASIATGLILWF-VPVPEGVTRNAWQLLAIFLAT 134

Query: 163 IAGLVLSPLPVGAWAFLGLTMTVVTKTLTFASAFNAFTSEVIWLIVISFFFARGFVKTGL 222
           I G++  PLP+GA A +GL  +V+TKTLTFA+AF+AF   + WLI ++FFFARGF+KTGL
Sbjct: 135 IVGIITQPLPLGAVALMGLGASVLTKTLTFAAAFSAFGDPIPWLIALAFFFARGFIKTGL 194

Query: 223 GDRIATYFVKWMGKSTLGLSYGLTFSEALIAPAMPSTTARAGGVFLPIIKSLSLAADSKP 282
           G+R+A  FV+  G S+LGL Y L FSEAL+APA+PS +ARAGG+FLP++KSL +A  S  
Sbjct: 195 GNRVAYQFVRLFGSSSLGLGYSLVFSEALLAPAIPSVSARAGGIFLPLVKSLCVACGSNV 254

Query: 283 NDPSSKKIGAYLIQSQFQSAGNSSALFLTAAAQNLLCLKLAEELGVQISSPWLTWAKIAS 342
            D +  ++G++L+ + FQ++  SS++FLTA A N L   LA    ++ +  W  WAK A 
Sbjct: 255 GDGTEHRLGSWLMLTCFQTSVISSSMFLTAMAANPLSANLAFNT-IKQTIGWTDWAKAAI 314

Query: 343 VPAIICLLTTPAILYKLFPPETKNTPDAPAMATKKLEAMGPVTKNEWVMVGTMLIAVSLW 402
           VP ++ L+  P +LY ++PP  K++PDAP +A +KL+ MGP++KNE +M  T+ + V LW
Sbjct: 315 VPGLVSLIVVPFLLYLIYPPTVKSSPDAPKLAQEKLDKMGPMSKNELIMAATLFLTVGLW 374

Query: 403 IAGEALQIPSVVAAMIGLSTLLTLGVLNWEDCLAEKSAWDTLAWFAVLVGMAGQLTSLGL 462
           I G  L + +V AA++GLS LL  GV+ W++CLAE  AWDTL WFA L+ MAG L   GL
Sbjct: 375 IFGAKLGVDAVTAAILGLSVLLVTGVVTWKECLAESVAWDTLTWFAALIAMAGYLNKYGL 434

Query: 463 VGWMSNCVASLLTSLSLSWPAAFAILQLSYFFVHYLFASQTGHVGALYSAFLAMHLAAKV 522
           + W S  V   +  L LSW  +F IL L YF+ HY FAS   H+GA+++AFL++  A   
Sbjct: 435 IEWFSQTVVKFVGGLGLSWQLSFGILVLLYFYTHYFFASGAAHIGAMFTAFLSVSTALGT 494

Query: 523 PGVLAALALAYNTNLFGALTHYSSGQAAVYYGAGYVDLPDVFKIGFVMALVNGIIWIVAG 579
           P   AAL LA+ +NL G LTHY  G A ++YGA YV L   +  GF++++VN +IW+  G
Sbjct: 495 PPYFAALVLAFLSNLMGGLTHYGIGSAPIFYGANYVPLAKWWGYGFLISIVNILIWLGVG 554

BLAST of Cp4.1LG14g05220 vs. NCBI nr
Match: XP_023552692.1 (dicarboxylate transporter 2.1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1099 bits (2843), Expect = 0.0
Identity = 578/578 (100.00%), Postives = 578/578 (100.00%), Query Frame = 0

Query: 1   MESFALQSVSTTASLPRHSPISRISLLHRRKTIPHSQSFPTSPCLYRSVSLFQSAPPIPQ 60
           MESFALQSVSTTASLPRHSPISRISLLHRRKTIPHSQSFPTSPCLYRSVSLFQSAPPIPQ
Sbjct: 1   MESFALQSVSTTASLPRHSPISRISLLHRRKTIPHSQSFPTSPCLYRSVSLFQSAPPIPQ 60

Query: 61  SPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAI 120
           SPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAI
Sbjct: 61  SPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAI 120

Query: 121 GLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTFAS 180
           GLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTFAS
Sbjct: 121 GLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTFAS 180

Query: 181 AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAP 240
           AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAP
Sbjct: 181 AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAP 240

Query: 241 AMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAA 300
           AMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAA
Sbjct: 241 AMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAA 300

Query: 301 QNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMA 360
           QNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMA
Sbjct: 301 QNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMA 360

Query: 361 TKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDC 420
           TKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDC
Sbjct: 361 TKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDC 420

Query: 421 LAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLLTSLSLSWPAAFAILQLSYFF 480
           LAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLLTSLSLSWPAAFAILQLSYFF
Sbjct: 421 LAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLLTSLSLSWPAAFAILQLSYFF 480

Query: 481 VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG 540
           VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG
Sbjct: 481 VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG 540

Query: 541 AGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY 578
           AGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY
Sbjct: 541 AGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY 578

BLAST of Cp4.1LG14g05220 vs. NCBI nr
Match: KAG7014870.1 (Dicarboxylate transporter 2.1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1093 bits (2826), Expect = 0.0
Identity = 573/578 (99.13%), Postives = 577/578 (99.83%), Query Frame = 0

Query: 1   MESFALQSVSTTASLPRHSPISRISLLHRRKTIPHSQSFPTSPCLYRSVSLFQSAPPIPQ 60
           MESFALQSVSTTASLPRHSPI+RISLLHRRK+IPHSQSFPTSPCLYRSVSLFQSAPPIPQ
Sbjct: 1   MESFALQSVSTTASLPRHSPIARISLLHRRKSIPHSQSFPTSPCLYRSVSLFQSAPPIPQ 60

Query: 61  SPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAI 120
           SPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAI
Sbjct: 61  SPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAI 120

Query: 121 GLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTFAS 180
           GLIVRFA+PKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTFAS
Sbjct: 121 GLIVRFAIPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTFAS 180

Query: 181 AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAP 240
           AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAP
Sbjct: 181 AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAP 240

Query: 241 AMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAA 300
           AMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAA
Sbjct: 241 AMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAA 300

Query: 301 QNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMA 360
           QNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMA
Sbjct: 301 QNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMA 360

Query: 361 TKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDC 420
           TKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDC
Sbjct: 361 TKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDC 420

Query: 421 LAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLLTSLSLSWPAAFAILQLSYFF 480
           LAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVA+LL SLSLSWPAAFAILQLSYFF
Sbjct: 421 LAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVATLLKSLSLSWPAAFAILQLSYFF 480

Query: 481 VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG 540
           VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG
Sbjct: 481 VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG 540

Query: 541 AGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY 578
           AGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY
Sbjct: 541 AGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY 578

BLAST of Cp4.1LG14g05220 vs. NCBI nr
Match: XP_022985012.1 (dicarboxylate transporter 2.1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1090 bits (2820), Expect = 0.0
Identity = 574/578 (99.31%), Postives = 575/578 (99.48%), Query Frame = 0

Query: 1   MESFALQSVSTTASLPRHSPISRISLLHRRKTIPHSQSFPTSPCLYRSVSLFQSAPPIPQ 60
           MESFALQSVSTTASLPRHSPI+RISLLHRRKTIPHSQSFPTSPCLYRSVSLFQSAPPIPQ
Sbjct: 1   MESFALQSVSTTASLPRHSPIARISLLHRRKTIPHSQSFPTSPCLYRSVSLFQSAPPIPQ 60

Query: 61  SPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAI 120
           S LFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAI
Sbjct: 61  SLLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAI 120

Query: 121 GLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTFAS 180
           GLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTF S
Sbjct: 121 GLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTFTS 180

Query: 181 AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAP 240
           AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAP
Sbjct: 181 AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAP 240

Query: 241 AMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAA 300
           AMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAA
Sbjct: 241 AMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAA 300

Query: 301 QNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMA 360
           QNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMA
Sbjct: 301 QNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMA 360

Query: 361 TKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDC 420
           TKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDC
Sbjct: 361 TKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDC 420

Query: 421 LAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLLTSLSLSWPAAFAILQLSYFF 480
           LAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLL SLSLSWPAAFAILQLSYFF
Sbjct: 421 LAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLLKSLSLSWPAAFAILQLSYFF 480

Query: 481 VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG 540
           VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG
Sbjct: 481 VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG 540

Query: 541 AGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY 578
           AGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY
Sbjct: 541 AGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY 578

BLAST of Cp4.1LG14g05220 vs. NCBI nr
Match: XP_022922946.1 (dicarboxylate transporter 2.1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1090 bits (2820), Expect = 0.0
Identity = 572/578 (98.96%), Postives = 575/578 (99.48%), Query Frame = 0

Query: 1   MESFALQSVSTTASLPRHSPISRISLLHRRKTIPHSQSFPTSPCLYRSVSLFQSAPPIPQ 60
           MESFALQSVSTTASLPRHSPI+RISL HRRK IPHSQSFPTSPCLYRSVSLFQSAPPIPQ
Sbjct: 1   MESFALQSVSTTASLPRHSPIARISLFHRRKPIPHSQSFPTSPCLYRSVSLFQSAPPIPQ 60

Query: 61  SPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAI 120
           SPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAI
Sbjct: 61  SPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAI 120

Query: 121 GLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTFAS 180
           GLIVRFA+PKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTFAS
Sbjct: 121 GLIVRFAIPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTFAS 180

Query: 181 AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAP 240
           AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAP
Sbjct: 181 AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAP 240

Query: 241 AMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAA 300
           AMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAA
Sbjct: 241 AMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAA 300

Query: 301 QNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMA 360
           QNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMA
Sbjct: 301 QNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMA 360

Query: 361 TKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDC 420
           TKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDC
Sbjct: 361 TKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDC 420

Query: 421 LAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLLTSLSLSWPAAFAILQLSYFF 480
           LAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVA+LL SLSLSWPAAFAILQLSYFF
Sbjct: 421 LAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVATLLKSLSLSWPAAFAILQLSYFF 480

Query: 481 VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG 540
           VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG
Sbjct: 481 VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG 540

Query: 541 AGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY 578
           AGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY
Sbjct: 541 AGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY 578

BLAST of Cp4.1LG14g05220 vs. NCBI nr
Match: KAG6576851.1 (Splicing factor 3B subunit 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1070 bits (2767), Expect = 0.0
Identity = 563/568 (99.12%), Postives = 566/568 (99.65%), Query Frame = 0

Query: 1   MESFALQSVSTTASLPRHSPISRISLLHRRKTIPHSQSFPTSPCLYRSVSLFQSAPPIPQ 60
           MESFALQSVSTTASLPRHSPI+RISLLHRRK IPHSQSFPTSPCLYRSVSLFQSAPPIPQ
Sbjct: 1   MESFALQSVSTTASLPRHSPIARISLLHRRKPIPHSQSFPTSPCLYRSVSLFQSAPPIPQ 60

Query: 61  SPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAI 120
           SPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAI
Sbjct: 61  SPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAI 120

Query: 121 GLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTFAS 180
           GLIVRFA+PKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTFAS
Sbjct: 121 GLIVRFAIPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTFAS 180

Query: 181 AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAP 240
           AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAP
Sbjct: 181 AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAP 240

Query: 241 AMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAA 300
           AMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAA
Sbjct: 241 AMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAA 300

Query: 301 QNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMA 360
           QNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMA
Sbjct: 301 QNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMA 360

Query: 361 TKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDC 420
           TKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDC
Sbjct: 361 TKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDC 420

Query: 421 LAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLLTSLSLSWPAAFAILQLSYFF 480
           LAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVA+LL SLSLSWPAAFAILQLSYFF
Sbjct: 421 LAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVATLLKSLSLSWPAAFAILQLSYFF 480

Query: 481 VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG 540
           VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG
Sbjct: 481 VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG 540

Query: 541 AGYVDLPDVFKIGFVMALVNGIIWIVAG 568
           AGYVDLPDVFKIGFVMALVNGIIWIVAG
Sbjct: 541 AGYVDLPDVFKIGFVMALVNGIIWIVAG 568

BLAST of Cp4.1LG14g05220 vs. ExPASy TrEMBL
Match: A0A6J1J3Q1 (dicarboxylate transporter 2.1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111483106 PE=3 SV=1)

HSP 1 Score: 1090 bits (2820), Expect = 0.0
Identity = 574/578 (99.31%), Postives = 575/578 (99.48%), Query Frame = 0

Query: 1   MESFALQSVSTTASLPRHSPISRISLLHRRKTIPHSQSFPTSPCLYRSVSLFQSAPPIPQ 60
           MESFALQSVSTTASLPRHSPI+RISLLHRRKTIPHSQSFPTSPCLYRSVSLFQSAPPIPQ
Sbjct: 1   MESFALQSVSTTASLPRHSPIARISLLHRRKTIPHSQSFPTSPCLYRSVSLFQSAPPIPQ 60

Query: 61  SPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAI 120
           S LFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAI
Sbjct: 61  SLLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAI 120

Query: 121 GLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTFAS 180
           GLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTF S
Sbjct: 121 GLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTFTS 180

Query: 181 AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAP 240
           AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAP
Sbjct: 181 AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAP 240

Query: 241 AMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAA 300
           AMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAA
Sbjct: 241 AMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAA 300

Query: 301 QNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMA 360
           QNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMA
Sbjct: 301 QNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMA 360

Query: 361 TKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDC 420
           TKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDC
Sbjct: 361 TKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDC 420

Query: 421 LAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLLTSLSLSWPAAFAILQLSYFF 480
           LAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLL SLSLSWPAAFAILQLSYFF
Sbjct: 421 LAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLLKSLSLSWPAAFAILQLSYFF 480

Query: 481 VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG 540
           VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG
Sbjct: 481 VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG 540

Query: 541 AGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY 578
           AGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY
Sbjct: 541 AGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY 578

BLAST of Cp4.1LG14g05220 vs. ExPASy TrEMBL
Match: A0A6J1E5I1 (dicarboxylate transporter 2.1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111430773 PE=3 SV=1)

HSP 1 Score: 1090 bits (2820), Expect = 0.0
Identity = 572/578 (98.96%), Postives = 575/578 (99.48%), Query Frame = 0

Query: 1   MESFALQSVSTTASLPRHSPISRISLLHRRKTIPHSQSFPTSPCLYRSVSLFQSAPPIPQ 60
           MESFALQSVSTTASLPRHSPI+RISL HRRK IPHSQSFPTSPCLYRSVSLFQSAPPIPQ
Sbjct: 1   MESFALQSVSTTASLPRHSPIARISLFHRRKPIPHSQSFPTSPCLYRSVSLFQSAPPIPQ 60

Query: 61  SPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAI 120
           SPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAI
Sbjct: 61  SPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAI 120

Query: 121 GLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTFAS 180
           GLIVRFA+PKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTFAS
Sbjct: 121 GLIVRFAIPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTFAS 180

Query: 181 AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAP 240
           AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAP
Sbjct: 181 AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAP 240

Query: 241 AMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAA 300
           AMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAA
Sbjct: 241 AMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAA 300

Query: 301 QNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMA 360
           QNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMA
Sbjct: 301 QNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMA 360

Query: 361 TKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDC 420
           TKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDC
Sbjct: 361 TKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDC 420

Query: 421 LAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLLTSLSLSWPAAFAILQLSYFF 480
           LAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVA+LL SLSLSWPAAFAILQLSYFF
Sbjct: 421 LAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVATLLKSLSLSWPAAFAILQLSYFF 480

Query: 481 VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG 540
           VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG
Sbjct: 481 VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG 540

Query: 541 AGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY 578
           AGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY
Sbjct: 541 AGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY 578

BLAST of Cp4.1LG14g05220 vs. ExPASy TrEMBL
Match: A0A1S3CHW4 (dicarboxylate transporter 2.1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501156 PE=3 SV=1)

HSP 1 Score: 984 bits (2543), Expect = 0.0
Identity = 521/578 (90.14%), Postives = 541/578 (93.60%), Query Frame = 0

Query: 1   MESFALQSVSTTASLPRHSPISRISLLHRRKTIPHSQSFPTSPCLYRSVSLFQSAPPIPQ 60
           ME+FALQSVSTT S   HSP SRISLLHR K I HS SFP+S  L RS+SLFQS PPI Q
Sbjct: 1   MENFALQSVSTTTSFSPHSPTSRISLLHRSKPISHSLSFPSSLHLSRSISLFQSTPPISQ 60

Query: 61  SPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAI 120
           SP F+S  SSLFNPLFNPKLRR   +RTSASQSP+ QPLPPTP P+QGAKPIPFLISIAI
Sbjct: 61  SP-FLSSRSSLFNPLFNPKLRRAFPMRTSASQSPDNQPLPPTPPPRQGAKPIPFLISIAI 120

Query: 121 GLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTFAS 180
           GLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLT+TVVTKTLTFA+
Sbjct: 121 GLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTVTVVTKTLTFAA 180

Query: 181 AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAP 240
           AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLT SEALIAP
Sbjct: 181 AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTISEALIAP 240

Query: 241 AMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAA 300
           AMPSTTARAGGVFLPIIKSLSLAADSKPN PSS+KIGAYLIQSQFQ AGNSSALFLTAAA
Sbjct: 241 AMPSTTARAGGVFLPIIKSLSLAADSKPNHPSSRKIGAYLIQSQFQCAGNSSALFLTAAA 300

Query: 301 QNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMA 360
           QNLLCLKLAEELGVQISSPWLTWAK A  PAII +L TPAILYKLFPPETK+TP+APAMA
Sbjct: 301 QNLLCLKLAEELGVQISSPWLTWAKFACAPAIIGILVTPAILYKLFPPETKDTPEAPAMA 360

Query: 361 TKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDC 420
           T+KLEAMGPVTKNEW+MVGTMLIAVSLWIAGEAL+IPSVVAAMIGLS LLTLGVLNW+DC
Sbjct: 361 TRKLEAMGPVTKNEWIMVGTMLIAVSLWIAGEALKIPSVVAAMIGLSILLTLGVLNWDDC 420

Query: 421 LAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLLTSLSLSWPAAFAILQLSYFF 480
           L+EKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLL SLSLSWPAAFAILQ SYFF
Sbjct: 421 LSEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLLKSLSLSWPAAFAILQASYFF 480

Query: 481 VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG 540
           VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG
Sbjct: 481 VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG 540

Query: 541 AGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY 578
           AGYVDLPDVFKIGFVMAL+NG IW V G IWWK+LGLY
Sbjct: 541 AGYVDLPDVFKIGFVMALINGFIWTVVGCIWWKILGLY 577

BLAST of Cp4.1LG14g05220 vs. ExPASy TrEMBL
Match: A0A0A0KZB4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G110580 PE=3 SV=1)

HSP 1 Score: 965 bits (2494), Expect = 0.0
Identity = 510/578 (88.24%), Postives = 535/578 (92.56%), Query Frame = 0

Query: 1   MESFALQSVSTTASLPRHSPISRISLLHRRKTIPHSQSFPTSPCLYRSVSLFQSAPPIPQ 60
           ME+FALQSVSTT S   HSP SR+SLLHR K I  S SFP+S    RS SL QS PPIPQ
Sbjct: 1   MENFALQSVSTTTSFSLHSPTSRLSLLHRSKPISQSLSFPSSLHFSRSTSLLQSTPPIPQ 60

Query: 61  SPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQQGAKPIPFLISIAI 120
           SP F+S   SLFNPLFNPKLR+   +RTSASQSP+ QP+PP+P P+QGAKPIPFLISIAI
Sbjct: 61  SP-FLSSRLSLFNPLFNPKLRQPFPMRTSASQSPDNQPVPPSPPPRQGAKPIPFLISIAI 120

Query: 121 GLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTLTFAS 180
           GLIVRFAVPKPVEVSA+AWQLLSIFLSTIAGLVLSPLPVGAWAFLGLT+TVVTKTLTFA+
Sbjct: 121 GLIVRFAVPKPVEVSAEAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTVTVVTKTLTFAA 180

Query: 181 AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEALIAP 240
           AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLT SEALIAP
Sbjct: 181 AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTISEALIAP 240

Query: 241 AMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFLTAAA 300
           AMPSTTARAGGVFLPIIKSLSLAADSKPN PSS+KIGAYLIQSQFQ AGNSSALFLTAAA
Sbjct: 241 AMPSTTARAGGVFLPIIKSLSLAADSKPNHPSSRKIGAYLIQSQFQCAGNSSALFLTAAA 300

Query: 301 QNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDAPAMA 360
           QNLLCLKLAEELGVQISSPWLTWAK A  PAII +L TPAILYKLFPPETK+TP+APAMA
Sbjct: 301 QNLLCLKLAEELGVQISSPWLTWAKFACAPAIIGILVTPAILYKLFPPETKDTPEAPAMA 360

Query: 361 TKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLNWEDC 420
            +KLEAMGPVTKNEW+MVGTMLIAVSLWIAGEAL+IPSVVAAMIGLS LLTLGVLNW+DC
Sbjct: 361 ARKLEAMGPVTKNEWIMVGTMLIAVSLWIAGEALKIPSVVAAMIGLSILLTLGVLNWDDC 420

Query: 421 LAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLLTSLSLSWPAAFAILQLSYFF 480
           L+EKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVA LL SLSLSWPAAFAILQ SYFF
Sbjct: 421 LSEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVAGLLKSLSLSWPAAFAILQASYFF 480

Query: 481 VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG 540
           VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG
Sbjct: 481 VHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAAVYYG 540

Query: 541 AGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY 578
           AGYV+LPDVFKIGFVMAL+NG IW V G IWWK+LGLY
Sbjct: 541 AGYVELPDVFKIGFVMALINGFIWTVVGCIWWKILGLY 577

BLAST of Cp4.1LG14g05220 vs. ExPASy TrEMBL
Match: A0A6J1CIT1 (dicarboxylate transporter 2.1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111011554 PE=3 SV=1)

HSP 1 Score: 906 bits (2341), Expect = 0.0
Identity = 500/582 (85.91%), Postives = 523/582 (89.86%), Query Frame = 0

Query: 1   MESFALQSVSTTASLPRHSPISRISLLHRRKTIPHSQSF--PTSPCLYRSVSLFQSAPPI 60
           MESFALQS+STT+S    SPISRISLL R + I HSQSF  P SP    S S      PI
Sbjct: 1   MESFALQSLSTTSSF---SPISRISLLRRPRPISHSQSFSFPISPNFSPSTSK-----PI 60

Query: 61  PQSPLFISRSSSLFNPLFNPKLRRTHQLRTSA--SQSPEKQPLPPTPLPQQGAKPIPFLI 120
              PLF S+SS LFN L  PK  R H +RT+A  SQSP+ Q   PTP P++GAKPIPFLI
Sbjct: 61  SHFPLFTSQSS-LFNSL--PKSHRYHPMRTTAQSSQSPDIQS--PTPPPRRGAKPIPFLI 120

Query: 121 SIAIGLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKTL 180
           SIA+GLIVRFAVPKPV+VS QAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLT+TVVTKTL
Sbjct: 121 SIAVGLIVRFAVPKPVDVSLQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTVTVVTKTL 180

Query: 181 TFASAFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSEA 240
           TFA+AFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLT SEA
Sbjct: 181 TFAAAFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTISEA 240

Query: 241 LIAPAMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALFL 300
           LIAPAMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYL+ SQFQ AGNSSALFL
Sbjct: 241 LIAPAMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLVLSQFQCAGNSSALFL 300

Query: 301 TAAAQNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPDA 360
           TAAAQNLLCLKLAEELGVQISSPWLTWAK ASVPAI+ LL TPAILYKLFPPETKNTPDA
Sbjct: 301 TAAAQNLLCLKLAEELGVQISSPWLTWAKFASVPAIVGLLVTPAILYKLFPPETKNTPDA 360

Query: 361 PAMATKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVLN 420
           PAMAT+KL AMGPVTKNEWVMVGTMLIAVSLWIAGEAL+IPSVVAAMIGLS LLTLGVL+
Sbjct: 361 PAMATRKLAAMGPVTKNEWVMVGTMLIAVSLWIAGEALKIPSVVAAMIGLSILLTLGVLD 420

Query: 421 WEDCLAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLLTSLSLSWPAAFAILQL 480
           W+DCLAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSN VA LL SLSLSWPAAFAILQ 
Sbjct: 421 WDDCLAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNSVAHLLKSLSLSWPAAFAILQA 480

Query: 481 SYFFVHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQAA 540
           SYF VHYLFASQTGHVGALYSAFLAMHLAAKVPG+LAALALAYNTNLFGALTHYSSGQAA
Sbjct: 481 SYFLVHYLFASQTGHVGALYSAFLAMHLAAKVPGLLAALALAYNTNLFGALTHYSSGQAA 540

Query: 541 VYYGAGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY 578
           VYYGAGYV+LPDVFK+GFVMALVN  IW V GGIWWK+LGLY
Sbjct: 541 VYYGAGYVELPDVFKMGFVMALVNATIWSVVGGIWWKILGLY 569

BLAST of Cp4.1LG14g05220 vs. TAIR 10
Match: AT5G64290.1 (dicarboxylate transport 2.1 )

HSP 1 Score: 773.9 bits (1997), Expect = 9.3e-224
Identity = 425/586 (72.53%), Postives = 478/586 (81.57%), Query Frame = 0

Query: 1   MESFALQSVSTTA-----SLPRHSPISRISLLHRRKTIPHSQSFPTSPCLYRSVSLFQSA 60
           MESFAL S+STTA     S   H   SR+SLL R      S   P S    RS+S+    
Sbjct: 1   MESFALHSLSTTATSTLLSHHHHHHPSRLSLLRRT-----SSRSPPSTISLRSLSV---- 60

Query: 61  PPIPQSPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEKQPLPPTPLPQ---QGAKPI 120
                          L  PL  P  R + ++  +A Q     P PP+P P    QGAK I
Sbjct: 61  -------------QPLSFPLLKPIPRFSTRI-AAAPQDNAPPPPPPSPSPSPSPQGAKLI 120

Query: 121 PFLISIAIGLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVV 180
           P ++SI++GLI+RFAVP P  V+ Q WQLLSIFLSTIAGLVLSPLPVGAWAF+GLT ++V
Sbjct: 121 PLILSISVGLILRFAVPVPEGVTPQGWQLLSIFLSTIAGLVLSPLPVGAWAFIGLTASIV 180

Query: 181 TKTLTFASAFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLT 240
           TKTL+F++AF+AFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKW+GKSTLGLSYGLT
Sbjct: 181 TKTLSFSAAFSAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWLGKSTLGLSYGLT 240

Query: 241 FSEALIAPAMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSS 300
            SEALIAPAMPSTTARAGG+FLPIIKSLSL+A SKPND SS+K+G+YLIQSQFQ AGNSS
Sbjct: 241 LSEALIAPAMPSTTARAGGIFLPIIKSLSLSAGSKPNDSSSRKLGSYLIQSQFQCAGNSS 300

Query: 301 ALFLTAAAQNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKN 360
           ALFLTAAAQNLLCLKLAEELGV IS+PW++W K AS+PAII LL TP ILYKL+PPETK+
Sbjct: 301 ALFLTAAAQNLLCLKLAEELGVVISNPWVSWFKAASLPAIISLLCTPLILYKLYPPETKD 360

Query: 361 TPDAPAMATKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTL 420
           TP+AP +A  KL+ MGPVTKNEW+MVGTML+AV+LWI GE L IPSVVAAMIGLS LL L
Sbjct: 361 TPEAPGIAATKLKQMGPVTKNEWIMVGTMLLAVTLWICGETLGIPSVVAAMIGLSILLVL 420

Query: 421 GVLNWEDCLAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLLTSLSLSWPAAFA 480
           GVLNW+DCL+EKSAWDTLAWFAVLVGMAGQLT+LG+V WMS+CVA +L SLSLSWPAAF 
Sbjct: 421 GVLNWDDCLSEKSAWDTLAWFAVLVGMAGQLTNLGVVTWMSDCVAKVLQSLSLSWPAAFG 480

Query: 481 ILQLSYFFVHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSS 540
           +LQ +YFF+HYLFASQTGHVGAL+SAFLAMH+AA VPG+LAALALAYNTNLFGALTHYSS
Sbjct: 481 LLQAAYFFIHYLFASQTGHVGALFSAFLAMHIAAGVPGILAALALAYNTNLFGALTHYSS 540

Query: 541 GQAAVYYGAGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY 579
           GQAAVYYGAGYVDLPDVFKIGFVMA +N IIW V G  WWK LGLY
Sbjct: 541 GQAAVYYGAGYVDLPDVFKIGFVMATINAIIWGVVGTFWWKFLGLY 563

BLAST of Cp4.1LG14g05220 vs. TAIR 10
Match: AT5G64280.1 (dicarboxylate transporter 2.2 )

HSP 1 Score: 683.7 bits (1763), Expect = 1.3e-196
Identity = 369/583 (63.29%), Postives = 443/583 (75.99%), Query Frame = 0

Query: 1   MESFALQSVSTTAS-LPRHSPISRISLLHRRKTIPHSQSFPTSPCLYRSVSLFQSAPPIP 60
           MES AL+S+S +AS L  H   S+   L     +P S S  TSP L              
Sbjct: 1   MESLALRSISLSASYLSLHRSSSKSFAL-----LPPSISVHTSPTL-------------- 60

Query: 61  QSPLFISRSSSLFNPLFNPKLRRTHQLRTSASQSPEK----QPLPPTPLPQQGAKPIPFL 120
                  RS S+ +P F         LR +AS  PE+    QP PP+P   QGAK IP  
Sbjct: 61  -------RSLSISSPRFT--------LRATASSLPEEQNKPQPPPPSPPQPQGAKLIPLA 120

Query: 121 ISIAIGLIVRFAVPKPVEVSAQAWQLLSIFLSTIAGLVLSPLPVGAWAFLGLTMTVVTKT 180
           IS++IGLIVRF +P+P +V++Q WQLLSIFL TI+GLVL PLPVGAWAF+GLT ++VTKT
Sbjct: 121 ISVSIGLIVRFLIPRPEQVTSQGWQLLSIFLFTISGLVLGPLPVGAWAFIGLTASIVTKT 180

Query: 181 LTFASAFNAFTSEVIWLIVISFFFARGFVKTGLGDRIATYFVKWMGKSTLGLSYGLTFSE 240
           L F++AF AFT+E+IWLI ISFFFARGF+KTGLGDRIATYFVKW+GKSTLGLSYGL F E
Sbjct: 181 LPFSTAFAAFTNELIWLIAISFFFARGFIKTGLGDRIATYFVKWLGKSTLGLSYGLAFCE 240

Query: 241 ALIAPAMPSTTARAGGVFLPIIKSLSLAADSKPNDPSSKKIGAYLIQSQFQSAGNSSALF 300
            L+   MPST ARAGGVFLP+IKSL+++A S P DPSS+K+G++LIQ+Q Q +G S A+ 
Sbjct: 241 TLMGLIMPSTMARAGGVFLPVIKSLAISAGSYPGDPSSRKLGSFLIQTQLQCSGASGAIL 300

Query: 301 LTAAAQNLLCLKLAEELGVQISSPWLTWAKIASVPAIICLLTTPAILYKLFPPETKNTPD 360
           LT+AAQNLLCLKLA E+GV IS+PW+TW K+ASVPA + LL TP I+YKL+PPE K+TP+
Sbjct: 301 LTSAAQNLLCLKLAREVGVVISNPWITWFKVASVPAFVSLLCTPLIIYKLYPPELKHTPE 360

Query: 361 APAMATKKLEAMGPVTKNEWVMVGTMLIAVSLWIAGEALQIPSVVAAMIGLSTLLTLGVL 420
           APA A KKLE +GP+TKNEW+M+G M   VSLW+ GEA+ I SVV+AMIGLSTLL LGV+
Sbjct: 361 APAAAAKKLERLGPITKNEWIMLGAMAFTVSLWVFGEAIGIASVVSAMIGLSTLLLLGVI 420

Query: 421 NWEDCLAEKSAWDTLAWFAVLVGMAGQLTSLGLVGWMSNCVASLLTSLSLSWPAAFAILQ 480
           NW+DCL++KSAWD+L WFAVL+GMAGQLT+LG+V WMS+CVA LL SLSL+WPA+F ILQ
Sbjct: 421 NWDDCLSDKSAWDSLTWFAVLIGMAGQLTNLGVVAWMSDCVAKLLQSLSLTWPASFIILQ 480

Query: 481 LSYFFVHYLFASQTGHVGALYSAFLAMHLAAKVPGVLAALALAYNTNLFGALTHYSSGQA 540
             Y  +HYLFASQTGH GALY  FLAM +AA VPGVLAAL LA+N NL GAL HYS G A
Sbjct: 481 ACYLLIHYLFASQTGHAGALYPPFLAMQIAAGVPGVLAALCLAFNNNLSGALAHYSGGPA 540

Query: 541 AVYYGAGYVDLPDVFKIGFVMALVNGIIWIVAGGIWWKVLGLY 579
           A+YYGAGYVDL D+F++GFVMALV  IIW   G  WWK LGLY
Sbjct: 541 ALYYGAGYVDLRDMFRVGFVMALVQAIIWGGVGSFWWKFLGLY 549

BLAST of Cp4.1LG14g05220 vs. TAIR 10
Match: AT5G12860.1 (dicarboxylate transporter 1 )

HSP 1 Score: 458.8 bits (1179), Expect = 6.6e-129
Identity = 264/550 (48.00%), Postives = 357/550 (64.91%), Query Frame = 0

Query: 43  PCLYRSVSLFQSAPPIPQSPLFI--------SRSSSLFNPLFNPKLRRTHQL------RT 102
           P   RS+SL    P  P S L +        SR S L +PL     RR+  L        
Sbjct: 15  PLKSRSLSL----PRPPSSSLNLTKPLRSLDSRFSLLKSPLPVSLRRRSSTLVKASSTVA 74

Query: 103 SASQSPEKQPLPPTPLPQQGAKPIPFLISIAIGLIVRFAVPKPVEVSAQAWQLLSIFLST 162
           SAS SP   PL P P+P QGA   P L SIA GLI+ F VP P  V+  AWQLL+IFL+T
Sbjct: 75  SASSSP-TPPLVPAPVPWQGAAIKPLLASIATGLILWF-VPVPEGVTRNAWQLLAIFLAT 134

Query: 163 IAGLVLSPLPVGAWAFLGLTMTVVTKTLTFASAFNAFTSEVIWLIVISFFFARGFVKTGL 222
           I G++  PLP+GA A +GL  +V+TKTLTFA+AF+AF   + WLI ++FFFARGF+KTGL
Sbjct: 135 IVGIITQPLPLGAVALMGLGASVLTKTLTFAAAFSAFGDPIPWLIALAFFFARGFIKTGL 194

Query: 223 GDRIATYFVKWMGKSTLGLSYGLTFSEALIAPAMPSTTARAGGVFLPIIKSLSLAADSKP 282
           G+R+A  FV+  G S+LGL Y L FSEAL+APA+PS +ARAGG+FLP++KSL +A  S  
Sbjct: 195 GNRVAYQFVRLFGSSSLGLGYSLVFSEALLAPAIPSVSARAGGIFLPLVKSLCVACGSNV 254

Query: 283 NDPSSKKIGAYLIQSQFQSAGNSSALFLTAAAQNLLCLKLAEELGVQISSPWLTWAKIAS 342
            D +  ++G++L+ + FQ++  SS++FLTA A N L   LA    ++ +  W  WAK A 
Sbjct: 255 GDGTEHRLGSWLMLTCFQTSVISSSMFLTAMAANPLSANLAFNT-IKQTIGWTDWAKAAI 314

Query: 343 VPAIICLLTTPAILYKLFPPETKNTPDAPAMATKKLEAMGPVTKNEWVMVGTMLIAVSLW 402
           VP ++ L+  P +LY ++PP  K++PDAP +A +KL+ MGP++KNE +M  T+ + V LW
Sbjct: 315 VPGLVSLIVVPFLLYLIYPPTVKSSPDAPKLAQEKLDKMGPMSKNELIMAATLFLTVGLW 374

Query: 403 IAGEALQIPSVVAAMIGLSTLLTLGVLNWEDCLAEKSAWDTLAWFAVLVGMAGQLTSLGL 462
           I G  L + +V AA++GLS LL  GV+ W++CLAE  AWDTL WFA L+ MAG L   GL
Sbjct: 375 IFGAKLGVDAVTAAILGLSVLLVTGVVTWKECLAESVAWDTLTWFAALIAMAGYLNKYGL 434

Query: 463 VGWMSNCVASLLTSLSLSWPAAFAILQLSYFFVHYLFASQTGHVGALYSAFLAMHLAAKV 522
           + W S  V   +  L LSW  +F IL L YF+ HY FAS   H+GA+++AFL++  A   
Sbjct: 435 IEWFSQTVVKFVGGLGLSWQLSFGILVLLYFYTHYFFASGAAHIGAMFTAFLSVSTALGT 494

Query: 523 PGVLAALALAYNTNLFGALTHYSSGQAAVYYGAGYVDLPDVFKIGFVMALVNGIIWIVAG 579
           P   AAL LA+ +NL G LTHY  G A ++YGA YV L   +  GF++++VN +IW+  G
Sbjct: 495 PPYFAALVLAFLSNLMGGLTHYGIGSAPIFYGANYVPLAKWWGYGFLISIVNILIWLGVG 554

BLAST of Cp4.1LG14g05220 vs. TAIR 10
Match: AT5G12860.2 (dicarboxylate transporter 1 )

HSP 1 Score: 458.0 bits (1177), Expect = 1.1e-128
Identity = 264/549 (48.09%), Postives = 356/549 (64.85%), Query Frame = 0

Query: 43  PCLYRSVSLFQSAPPIPQSPLFI--------SRSSSLFNPLFNPKLRRTHQL------RT 102
           P   RS+SL    P  P S L +        SR S L +PL     RR+  L        
Sbjct: 15  PLKSRSLSL----PRPPSSSLNLTKPLRSLDSRFSLLKSPLPVSLRRRSSTLVKASSTVA 74

Query: 103 SASQSPEKQPLPPTPLPQQGAKPIPFLISIAIGLIVRFAVPKPVEVSAQAWQLLSIFLST 162
           SAS SP   PL P P+P QGA   P L SIA GLI+ F VP P  V+  AWQLL+IFL+T
Sbjct: 75  SASSSP-TPPLVPAPVPWQGAAIKPLLASIATGLILWF-VPVPEGVTRNAWQLLAIFLAT 134

Query: 163 IAGLVLSPLPVGAWAFLGLTMTVVTKTLTFASAFNAFTSEVIWLIVISFFFARGFVKTGL 222
           I G++  PLP+GA A +GL  +V+TKTLTFA+AF+AF   + WLI ++FFFARGF+KTGL
Sbjct: 135 IVGIITQPLPLGAVALMGLGASVLTKTLTFAAAFSAFGDPIPWLIALAFFFARGFIKTGL 194

Query: 223 GDRIATYFVKWMGKSTLGLSYGLTFSEALIAPAMPSTTARAGGVFLPIIKSLSLAADSKP 282
           G+R+A  FV+  G S+LGL Y L FSEAL+APA+PS +ARAGG+FLP++KSL +A  S  
Sbjct: 195 GNRVAYQFVRLFGSSSLGLGYSLVFSEALLAPAIPSVSARAGGIFLPLVKSLCVACGSNV 254

Query: 283 NDPSSKKIGAYLIQSQFQSAGNSSALFLTAAAQNLLCLKLAEELGVQISSPWLTWAKIAS 342
            D +  ++G++L+ + FQ++  SS++FLTA A N L   LA    ++ +  W  WAK A 
Sbjct: 255 GDGTEHRLGSWLMLTCFQTSVISSSMFLTAMAANPLSANLAFNT-IKQTIGWTDWAKAAI 314

Query: 343 VPAIICLLTTPAILYKLFPPETKNTPDAPAMATKKLEAMGPVTKNEWVMVGTMLIAVSLW 402
           VP ++ L+  P +LY ++PP  K++PDAP +A +KL+ MGP++KNE +M  T+ + V LW
Sbjct: 315 VPGLVSLIVVPFLLYLIYPPTVKSSPDAPKLAQEKLDKMGPMSKNELIMAATLFLTVGLW 374

Query: 403 IAGEALQIPSVVAAMIGLSTLLTLGVLNWEDCLAEKSAWDTLAWFAVLVGMAGQLTSLGL 462
           I G  L + +V AA++GLS LL  GV+ W++CLAE  AWDTL WFA L+ MAG L   GL
Sbjct: 375 IFGAKLGVDAVTAAILGLSVLLVTGVVTWKECLAESVAWDTLTWFAALIAMAGYLNKYGL 434

Query: 463 VGWMSNCVASLLTSLSLSWPAAFAILQLSYFFVHYLFASQTGHVGALYSAFLAMHLAAKV 522
           + W S  V   +  L LSW  +F IL L YF+ HY FAS   H+GA+++AFL++  A   
Sbjct: 435 IEWFSQTVVKFVGGLGLSWQLSFGILVLLYFYTHYFFASGAAHIGAMFTAFLSVSTALGT 494

Query: 523 PGVLAALALAYNTNLFGALTHYSSGQAAVYYGAGYVDLPDVFKIGFVMALVNGIIWIVAG 578
           P   AAL LA+ +NL G LTHY  G A ++YGA YV L   +  GF++++VN +IW+  G
Sbjct: 495 PPYFAALVLAFLSNLMGGLTHYGIGSAPIFYGANYVPLAKWWGYGFLISIVNILIWLGVG 554

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FMF71.3e-22272.53Dicarboxylate transporter 2.1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8L7Z94.4e-21068.61Dicarboxylate transporter 2, chloroplastic OS=Spinacia oleracea OX=3562 GN=DIT2 ... [more]
Q9FMF81.8e-19563.29Dicarboxylate transporter 2.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q413648.2e-13247.50Dicarboxylate transporter 1, chloroplastic OS=Spinacia oleracea OX=3562 GN=DIT1 ... [more]
Q9LXV39.4e-12848.00Dicarboxylate transporter 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DI... [more]
Match NameE-valueIdentityDescription
XP_023552692.10.0100.00dicarboxylate transporter 2.1, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
KAG7014870.10.099.13Dicarboxylate transporter 2.1, chloroplastic [Cucurbita argyrosperma subsp. argy... [more]
XP_022985012.10.099.31dicarboxylate transporter 2.1, chloroplastic-like [Cucurbita maxima][more]
XP_022922946.10.098.96dicarboxylate transporter 2.1, chloroplastic-like [Cucurbita moschata][more]
KAG6576851.10.099.12Splicing factor 3B subunit 1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1J3Q10.099.31dicarboxylate transporter 2.1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1E5I10.098.96dicarboxylate transporter 2.1, chloroplastic-like OS=Cucurbita moschata OX=3662 ... [more]
A0A1S3CHW40.090.14dicarboxylate transporter 2.1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10350... [more]
A0A0A0KZB40.088.24Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G110580 PE=3 SV=1[more]
A0A6J1CIT10.085.91dicarboxylate transporter 2.1, chloroplastic OS=Momordica charantia OX=3673 GN=L... [more]
Match NameE-valueIdentityDescription
AT5G64290.19.3e-22472.53dicarboxylate transport 2.1 [more]
AT5G64280.11.3e-19663.29dicarboxylate transporter 2.2 [more]
AT5G12860.16.6e-12948.00dicarboxylate transporter 1 [more]
AT5G12860.21.1e-12848.09dicarboxylate transporter 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001898Solute carrier family 13PFAMPF00939Na_sulph_sympcoord: 114..577
e-value: 6.8E-124
score: 414.5
IPR001898Solute carrier family 13TIGRFAMTIGR00785TIGR00785coord: 130..572
e-value: 6.5E-111
score: 369.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 85..104
NoneNo IPR availablePANTHERPTHR42826:SF7DICARBOXYLIC ACID TRANSPORTER2coord: 82..578
IPR030676Citrate carrier CitT-relatedPANTHERPTHR42826DICARBOXYLATE TRANSPORTER 2.1, CHLOROPLASTICcoord: 82..578

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG14g05220.1Cp4.1LG14g05220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0009706 chloroplast inner membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity