Cp4.1LG14g05000 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG14g05000
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionERAD-associated E3 ubiquitin-protein ligase HRD1B-like
LocationCp4.1LG14: 1248225 .. 1253243 (-)
RNA-Seq ExpressionCp4.1LG14g05000
SyntenyCp4.1LG14g05000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGTGCAGTCGAGAATGATGAAGCTGCAAACATATGCGGGGATTAGTTTCATTGCAACCATAGTGGTTATCTATCATGCCTTCAACAGTAGAGGCCAGTTCTACCCAGCACTGGTCTATTTGTCAACTTCCAAGATAAATCTAGTGCTTCTTCTCAATATGGGTCTAGTCATCATGTGCATTTTGTGGCAGCTAACAAAGAAAATATTTCTCGGTTCTCTTAGAGAAGCAGAGGTTGAGAGGCTCAACGAGCAATCATGGAAGGAGTTGATGGAAATCCTCTTTGCCATAACTATTTTCCGGCAAGATTTTTCTGTTTCATTCCTTGGTATGGTTACGGCTCTGTTGTTGATCAAAGCTTTGCATTGGTTGGCTCAGAAGAGGGTTGAATACATGGAAACCACTCCATCGGTGCCGTTGTTGTCTCATATCCGGATAGTTTCCTTCTTAGGCTTCCTCTTTGTTCTAGATAGTCTGTTTTTTTATAATTCGATAGAATCTCTGATACAGACAAGGAAGGCCTCGGTTTCCCTCTTCTTTTCATTTGAGTAAGTTGTCATTTTCCACCGTAAGTTATATTATCTTTCTTCTTCTTCTTCTTTTTTATTTTTCTTTTGTAACAAACATAAATATTTCATTGATATGATGAAATTACAAGAGGAAAGTCCTATCCAATGGATTACAAAAAGCGTTTCCAATTGATCATGGGATTTTTAACCTATAATTGGTCATGAGATTTGGTCTATATCTTGATTCTATATTTATACCAGTTTAAATTATGATGTTCATAGATTTGAAATTTATTGAACGTTTGGTACCCTAAAAATTTCATCAAGGCATTATCTGTGGTTAGAATGTCAGAGATGCCAAGGACATGTTAGATGAACTCACAAACTATTGTTTATATTTGTTATTTAAGTTCTTAGTGGAGAATTGTGGCTATTGGAATACTATCTTGTAGTCTTTGTTTGCTAGAGCTAAGTTTTCAAATATCCTATGATGTTTTCTTATGACCCTTAGAAGCTCTTATATCATCATAAAATAAAGTCAATGACTTGTTTTTCAGGTATATGATTCTTGCAACAACGGCTGTGTCAACGTTTGTAAAATATATTTTCTACGTCAGTGATGTACTCATGGAGGGACAATGGGAAAGGAAGCCCGTCTATACATTTTACTTGGAACTTATCCGAGACTTACTTCACTTGTCAATGTACCTGTGTTTCTTTCTTGTGATTTTCATGTAAGTACTCTAGCTACACCTGTTAAGCTATCTCATTCTTGTGAACTGGAAGTTCAGAATTAATTTCTTCTGCATATATGTGCTCATATTGCGTATGCCCAACTGTTAAAGTAGTAGAACATTGGTTCTTCTAAAGAAATACTATGCAAACCAATAAAAAACGAATGTGGTTACTAGGATTTGTGTCCTTACATGTATCAATGGCTGTCGTATTTTGCTCTCATGTTAATTGTGGGAGAAGTCTCATAAGTCATAACAGTAGTGTCTTATGACTGACATGGTCTTTGTTGTTTGTTTGGATATCCTTTTCATTTCTTTCCAATCAGTCGTTGTTCTTAATTTCTTTCCTCTTTTAAGATTGAAAGCGGCAGTTGTTTTTGTAAATAAGTCAATTACTATCCCAAAGGTTGATGGTTCGATCCTCAACCTAAAAAGTTGAAGTTACATCCATATCAACTTCATCCCCATTTCACATCTTGATTTTGCTGACTTTGACTTTGCTCTTGCAGGACCTATGGTGTGCCTTTGCACTTGATCCGAGAGCTCTATGAGATCTTTAGGAATTTTAGGATTCGTATTGCAGATTACATCCGTTACAGAAAGATTACTTCAAACATGAATGATCGATTTCCCGATGCAACGCCTGAAGAACTCAATTCGTGCGTACATGCTTGGTCAACTTGTTGTTTCTTTTATGCTTCCAGTACTTTTATATTGGCTACTAAAAATTCCTTCTATGCAATTGTAGAAGTGATGCAACATGCATTATCTGTCGTGAAGAGATGACAGTTGCAAAGAAACTAGTATGTGGACATCTTTTTCATGTCCATTGCCTCCGATCCTGGTTGGAGCGACAACATACTTGCCCAACCTGCAGAGCCCTTGTTGTACCACCTGAAGGTTCAAGCACAGCAGCCGGTCATCATGGACCACAATCTGATGCTAACCAACAAGGTAATTGCACCAATAAGAATTCAATTCATTTCAATTTACTGCCTGGCTAAAGGATCCTACATCTTGTACAATTATGATAAGTTTATAAATTTCTTCCGTGTATATTTCATCTGGTTGTGAACCTTATTGGAATCTTTCATTTGAGGAAACTGAAACTTCGACTAAGGAAAAGCATGAAAGATCCAGAAGATCTAAGCTAAAAGAGTAATCTTTGATGTTGCTTATGTTTCATTCAGAAACCTAAAGATAGGAAATATGAATCTTCTCAATGTTCTAGGGACAGGAAAATATCTTTTCCACCTGCCTTGTCTCCCACGAATTAGTTTGTGCAATGCTTTTTTAGTATCTTGTTTGTGCAATGCTTCCCCTGCATCTCTGATTCTTTTTTACAAGTGGTGCTTTGTATCTGGCATCTAGTAACCCAATTTGGTTTAACGTTTCAAAACTATTAAATAATCTAGACCATTAATAACCATGCAAAAATAAGCATCAGTATAGTATTTCCAAGTCTTCTCCATATATATACCTCCCTATCAGTGTGAAAGAGTTGAAATTTGGGTCTCTCTCACAATTCATTTCATGGTTGTTGACTTTCTCTCTATTCTCTTCTCTATTATTATTTCTTTTGTATTCATGATTGTTTTTCTTATGAGACTGTCCTTAAAGTCGTCGTTTATTTTATATATTTTTTTGTCCTACAACTCAATCTCTTCAAAAGGAAATAGTTTGAAGAACCGGCTATGAACTTTAGCATATCTCTTTGAGAAGGATGATTATTCTTGAACTGGGCCTCTGGTCCTCTGTAAAAATTGGGCGCTTCATTTTTTTCTTGTTTTGTTTTTAACTTTTTTGTTGTGTTAGAAAAGGTGTAGGAGTAATTTAGATAAGGTGTAGAAGTAATTTCCAAGTTTACATGATCTAATGGCTTTGGATTAAAATGTTTTGTTGTTTGGTGCATTCTTATAGCATTCCCTTCAGTGTATCTGCTTTCTTTAATAGAAGTTTTTTACTCAAATCATTGAACTGATGGTTTTACTTGATTATTTTTCAATATGGGGTTATACCATGCTGGAACTTGGATACAATGCAATGACTGATCTTTCTGTTGAAAACAGGAGCAGGAACCACAAGTTCATCCGCGCAAGGTACGAGTGATACAGGTGTTGCTAATGATAATTTGAGCCATCATCAGGCTAGAGTCCGAGCTGCTGTGGCTGCAGCTTCTGTATATGAGAAATCATATGTTTATCCTTGTGCGAGCACATTAGTATGGTACGCGTTTTTATTGCAAACATCCCACACACGCACCCACCAAAAGGAGAAGAGTATGACAAATTTTTTTAATTATTTTTATTTATTTACAGGTCTCCTGGATATGCAGTAGTTCCTCAGGTTGAAAGACCTTTGCCTGACTCCAATAACATAGAACCAAATGGACGTCCACAACAATTTTCTTTTACTGGCGGCCCTTCCAACTTGTCCTTCACTCAATTTCCCCAGAATCTTTTTGTTCCTTTTCAGCCACGAGAGGATACTGGAAACAGTACGGACGGATTGGGCAGTAGTCAAAATGTATCAGGCTTACATCTTGAAGCTCAACAAAGATTTCTCCAGCAACAGATTGAGGTATGTTGTTTAATTGTTGCATCAACGTTGTCTAGTTGGGTCTCATTCTTCTTGATGGAGCCTTTTTCTCTAGTTGGGCTCCCTTTTGTGGTCTTGTTTTTTTGGGTTGCCTTGTATTCTTTCATTTTTTTTTTCTCATTAAAAAATATCACTACCCAATGAACTCTCATGTTTGGTCAGTGTATGTTTCGTTTGTGCTCTTGTTGCCTGGGCCTCTGTATATTCTTTAGCTCAGTTTTTCAAGGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAANAACAGTAGTTTACTAGTATGCACATGGCCGTGAGTAAATATTGAATTGTGGTCATGACATGTGCTTAGGTACTTGATTCTCAGCGTTGTTTGGTTTTCCCCTTTGTGCTGTTTCCTTGACCTCTGATGTTCAACGACGAGAAGCCAACTTTTTTGCTTTTATATTCGTGGTTTACCGTTGTGGTGTTTGAAAATGAGCTACCATAAACAGTTAAAAAGAAAAAGAAAAAAATTGTGATACTTAAGGAGATGTCCTATTAATATATAAATTTGTATTTATTAGGTAAAGCATATGAAATTCATTCGTTCTCTGTTCTCTTTTCTTTTCTTTCACCGTAAATACGGCTCTTCGTTCAATTCTTTCAAAGTCACTGACAACTTCATTCAAACTAGTTTTAGTAGAGGTTGAGGATTTCTGAATTCAAAATTTCATGTTTTCCAACAGATCCTACAGAATCAGCTGCAACATCTCCGTAAACCAGATGTTGAGGAAAGCTCAGGAGCAGGTGAGGCAGATAAGAAAGGGAAAAGTGTAGCATTCTCGTTGTCGCCATCGCCGTCGTCATCTGATTCTCGATGAGATAGAAGAAATGATGTGCATATAATTATATTTGTGTAATAATACAAGACTGCAGAGGTTCAGAATGACATAGATATCTCTTTTTGTATACGGAAGATGTTTTTGGTATATTTTTAGAAGCAGTCTTCTTCCCCGTATTGGTTGGTTGGTTTGGTTGTAAATGCTGAATTTGGCGTCTCTTTTGAAGCAATTATGCGACGGTCAAAAAGGAATATTTAAAGTTCTTTCTACCAAAAGAAGAAGAGAGTGAGTTTATTCCAACATCTTTGGTTTCTCTATTTTCTTACTCTAAAATATGCTTTTTGAAAAGCAATTCCATTCCCATGCGCATTTGTT

mRNA sequence

ATGGCAGTGCAGTCGAGAATGATGAAGCTGCAAACATATGCGGGGATTAGTTTCATTGCAACCATAGTGGTTATCTATCATGCCTTCAACAGTAGAGGCCAGTTCTACCCAGCACTGGTCTATTTGTCAACTTCCAAGATAAATCTAGTGCTTCTTCTCAATATGGGTCTAGTCATCATGTGCATTTTGTGGCAGCTAACAAAGAAAATATTTCTCGGTTCTCTTAGAGAAGCAGAGGTTGAGAGGCTCAACGAGCAATCATGGAAGGAGTTGATGGAAATCCTCTTTGCCATAACTATTTTCCGGCAAGATTTTTCTGTTTCATTCCTTGGTATGGTTACGGCTCTGTTGTTGATCAAAGCTTTGCATTGGTTGGCTCAGAAGAGGGTTGAATACATGGAAACCACTCCATCGGTGCCGTTGTTGTCTCATATCCGGATAGTTTCCTTCTTAGGCTTCCTCTTTGTTCTAGATAGTCTGTTTTTTTATAATTCGATAGAATCTCTGATACAGACAAGGAAGGCCTCGGTTTCCCTCTTCTTTTCATTTGAGTATATGATTCTTGCAACAACGGCTGTGTCAACGTTTGTAAAATATATTTTCTACGTCAGTGATGTACTCATGGAGGGACAATGGGAAAGGAAGCCCGTCTATACATTTTACTTGGAACTTATCCGAGACTTACTTCACTTGTCAATGTACCTGTGTTTCTTTCTTGTGATTTTCATGACCTATGGTGTGCCTTTGCACTTGATCCGAGAGCTCTATGAGATCTTTAGGAATTTTAGGATTCGTATTGCAGATTACATCCGTTACAGAAAGATTACTTCAAACATGAATGATCGATTTCCCGATGCAACGCCTGAAGAACTCAATTCAAGTGATGCAACATGCATTATCTGTCGTGAAGAGATGACAGTTGCAAAGAAACTAGTATGTGGACATCTTTTTCATGTCCATTGCCTCCGATCCTGGTTGGAGCGACAACATACTTGCCCAACCTGCAGAGCCCTTGTTGTACCACCTGAAGGTTCAAGCACAGCAGCCGGTCATCATGGACCACAATCTGATGCTAACCAACAAGGAGCAGGAACCACAAGTTCATCCGCGCAAGGTACGAGTGATACAGGTGTTGCTAATGATAATTTGAGCCATCATCAGGCTAGAGTCCGAGCTGCTGTGGCTGCAGCTTCTGTATATGAGAAATCATATGTTTATCCTTGTGCGAGCACATTAGTATGGTCTCCTGGATATGCAGTAGTTCCTCAGGTTGAAAGACCTTTGCCTGACTCCAATAACATAGAACCAAATGGACGTCCACAACAATTTTCTTTTACTGGCGGCCCTTCCAACTTGTCCTTCACTCAATTTCCCCAGAATCTTTTTGTTCCTTTTCAGCCACGAGAGGATACTGGAAACAGTACGGACGGATTGGGCAGTAGTCAAAATGTATCAGGCTTACATCTTGAAGCTCAACAAAGATTTCTCCAGCAACAGATTGAGATCCTACAGAATCAGCTGCAACATCTCCGTAAACCAGATGTTGAGGAAAGCTCAGGAGCAGGTGAGGCAGATAAGAAAGGGAAAAGTGTAGCATTCTCGTTGTCGCCATCGCCGTCGTCATCTGATTCTCGATGAGATAGAAGAAATGATGTGCATATAATTATATTTGTGTAATAATACAAGACTGCAGAGGTTCAGAATGACATAGATATCTCTTTTTGTATACGGAAGATGTTTTTGGTATATTTTTAGAAGCAGTCTTCTTCCCCGTATTGGTTGGTTGGTTTGGTTGTAAATGCTGAATTTGGCGTCTCTTTTGAAGCAATTATGCGACGGTCAAAAAGGAATATTTAAAGTTCTTTCTACCAAAAGAAGAAGAGAGTGAGTTTATTCCAACATCTTTGGTTTCTCTATTTTCTTACTCTAAAATATGCTTTTTGAAAAGCAATTCCATTCCCATGCGCATTTGTT

Coding sequence (CDS)

ATGGCAGTGCAGTCGAGAATGATGAAGCTGCAAACATATGCGGGGATTAGTTTCATTGCAACCATAGTGGTTATCTATCATGCCTTCAACAGTAGAGGCCAGTTCTACCCAGCACTGGTCTATTTGTCAACTTCCAAGATAAATCTAGTGCTTCTTCTCAATATGGGTCTAGTCATCATGTGCATTTTGTGGCAGCTAACAAAGAAAATATTTCTCGGTTCTCTTAGAGAAGCAGAGGTTGAGAGGCTCAACGAGCAATCATGGAAGGAGTTGATGGAAATCCTCTTTGCCATAACTATTTTCCGGCAAGATTTTTCTGTTTCATTCCTTGGTATGGTTACGGCTCTGTTGTTGATCAAAGCTTTGCATTGGTTGGCTCAGAAGAGGGTTGAATACATGGAAACCACTCCATCGGTGCCGTTGTTGTCTCATATCCGGATAGTTTCCTTCTTAGGCTTCCTCTTTGTTCTAGATAGTCTGTTTTTTTATAATTCGATAGAATCTCTGATACAGACAAGGAAGGCCTCGGTTTCCCTCTTCTTTTCATTTGAGTATATGATTCTTGCAACAACGGCTGTGTCAACGTTTGTAAAATATATTTTCTACGTCAGTGATGTACTCATGGAGGGACAATGGGAAAGGAAGCCCGTCTATACATTTTACTTGGAACTTATCCGAGACTTACTTCACTTGTCAATGTACCTGTGTTTCTTTCTTGTGATTTTCATGACCTATGGTGTGCCTTTGCACTTGATCCGAGAGCTCTATGAGATCTTTAGGAATTTTAGGATTCGTATTGCAGATTACATCCGTTACAGAAAGATTACTTCAAACATGAATGATCGATTTCCCGATGCAACGCCTGAAGAACTCAATTCAAGTGATGCAACATGCATTATCTGTCGTGAAGAGATGACAGTTGCAAAGAAACTAGTATGTGGACATCTTTTTCATGTCCATTGCCTCCGATCCTGGTTGGAGCGACAACATACTTGCCCAACCTGCAGAGCCCTTGTTGTACCACCTGAAGGTTCAAGCACAGCAGCCGGTCATCATGGACCACAATCTGATGCTAACCAACAAGGAGCAGGAACCACAAGTTCATCCGCGCAAGGTACGAGTGATACAGGTGTTGCTAATGATAATTTGAGCCATCATCAGGCTAGAGTCCGAGCTGCTGTGGCTGCAGCTTCTGTATATGAGAAATCATATGTTTATCCTTGTGCGAGCACATTAGTATGGTCTCCTGGATATGCAGTAGTTCCTCAGGTTGAAAGACCTTTGCCTGACTCCAATAACATAGAACCAAATGGACGTCCACAACAATTTTCTTTTACTGGCGGCCCTTCCAACTTGTCCTTCACTCAATTTCCCCAGAATCTTTTTGTTCCTTTTCAGCCACGAGAGGATACTGGAAACAGTACGGACGGATTGGGCAGTAGTCAAAATGTATCAGGCTTACATCTTGAAGCTCAACAAAGATTTCTCCAGCAACAGATTGAGATCCTACAGAATCAGCTGCAACATCTCCGTAAACCAGATGTTGAGGAAAGCTCAGGAGCAGGTGAGGCAGATAAGAAAGGGAAAAGTGTAGCATTCTCGTTGTCGCCATCGCCGTCGTCATCTGATTCTCGATGA

Protein sequence

MAVQSRMMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQLTKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLAQKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYMILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYGVPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMTVAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQGAGTTSSSAQGTSDTGVANDNLSHHQARVRAAVAAASVYEKSYVYPCASTLVWSPGYAVVPQVERPLPDSNNIEPNGRPQQFSFTGGPSNLSFTQFPQNLFVPFQPREDTGNSTDGLGSSQNVSGLHLEAQQRFLQQQIEILQNQLQHLRKPDVEESSGAGEADKKGKSVAFSLSPSPSSSDSR
Homology
BLAST of Cp4.1LG14g05000 vs. ExPASy Swiss-Prot
Match: Q6NPT7 (ERAD-associated E3 ubiquitin-protein ligase HRD1B OS=Arabidopsis thaliana OX=3702 GN=HRD1B PE=2 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 7.4e-175
Identity = 335/528 (63.45%), Postives = 396/528 (75.00%), Query Frame = 0

Query: 7   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 66
           M++L+ YAG+S +AT+VVIYHAF+SRGQFYPA VYLSTSKINLV+LLNMGLV+M  LW L
Sbjct: 1   MIQLKVYAGLSTLATLVVIYHAFSSRGQFYPATVYLSTSKINLVVLLNMGLVLMLSLWNL 60

Query: 67  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 126
            K +FLGSLREAEVERLNEQ+W+ELMEILFAITIFRQDFSV F+ +V  LLLIK LHW+A
Sbjct: 61  VKIVFLGSLREAEVERLNEQAWRELMEILFAITIFRQDFSVGFISLVVTLLLIKGLHWMA 120

Query: 127 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 186
           QKRVEY+ETTPSV LLSH+RIVSF+ FL +LD L  Y+SI+ LIQ+RKAS+S+FF+FEYM
Sbjct: 121 QKRVEYIETTPSVTLLSHVRIVSFMVFLLILDCLLTYSSIQQLIQSRKASMSVFFTFEYM 180

Query: 187 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 246
           ILATT VS  VKY FYV+D+L EGQWE KPVYTFYLEL+RDLLHLSMYLCFFL+IFM YG
Sbjct: 181 ILATTTVSIIVKYAFYVTDMLKEGQWEGKPVYTFYLELVRDLLHLSMYLCFFLMIFMNYG 240

Query: 247 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 306
           +PLHLIRELYE FRNF+IR+ DY+RYRKITSNMNDRFPDATPEEL+S+DATCIICREEMT
Sbjct: 241 LPLHLIRELYETFRNFKIRVTDYLRYRKITSNMNDRFPDATPEELSSNDATCIICREEMT 300

Query: 307 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPE-GSSTAAGHHGPQSDANQQGAGT 366
            AKKLVCGHLFHVHCLRSWLERQ+TCPTCRALVVP E  +STA+G+ GP  ++ QQG GT
Sbjct: 301 SAKKLVCGHLFHVHCLRSWLERQNTCPTCRALVVPAENATSTASGNRGPHQESLQQGTGT 360

Query: 367 TSSSAQGTSDTGVANDNLSHHQARVRAAVAAASVYEKSYVYP-CASTLVWSPGYAVVPQV 426
           +SS  QG+S +  A++N+S H+AR +AA +AAS+Y +S VYP  A+TLVWS G +++PQ 
Sbjct: 361 SSSDGQGSSVSAAASENMSRHEARFQAAASAASIYGRSIVYPSSANTLVWSQGNSLLPQT 420

Query: 427 ERPLPDSNNIEPNGRPQQFSFTGGPSNLSFTQFPQNLFVPFQPREDTGNSTDGLGSSQNV 486
           E                                                           
Sbjct: 421 E----------------------------------------------------------- 457

Query: 487 SGLHLEAQQRFLQQQIEILQNQLQHLRKPDVEESSGAGEADKKGKSVA 533
               +EAQ+RFL+ QIE+L NQL+ L KP           D KGKSVA
Sbjct: 481 ----VEAQRRFLESQIEVLTNQLRLLEKPTT--------VDTKGKSVA 457

BLAST of Cp4.1LG14g05000 vs. ExPASy Swiss-Prot
Match: Q9LW77 (ERAD-associated E3 ubiquitin-protein ligase HRD1A OS=Arabidopsis thaliana OX=3702 GN=HRD1A PE=2 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 3.1e-173
Identity = 341/531 (64.22%), Postives = 403/531 (75.89%), Query Frame = 0

Query: 7   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 66
           M++L+TYAG+SF+AT+ VIYHAF+SRGQFYPA VYLSTSKI+LVLLLNM LV+M  LW L
Sbjct: 1   MIRLRTYAGLSFMATLAVIYHAFSSRGQFYPATVYLSTSKISLVLLLNMCLVLMLSLWHL 60

Query: 67  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 126
            K +FLGSLREAEVERLNEQ+W+ELMEILFAITIFRQDFS  FL +V  LLLIKALHWLA
Sbjct: 61  VKFVFLGSLREAEVERLNEQAWRELMEILFAITIFRQDFSSGFLPLVVTLLLIKALHWLA 120

Query: 127 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 186
           QKRVEY+ETTPSV  LSH RIVSF+GFL ++DSLF Y+SI  LIQ+R+ASVSLFFSFEYM
Sbjct: 121 QKRVEYIETTPSVSKLSHFRIVSFMGFLLLVDSLFMYSSIRHLIQSRQASVSLFFSFEYM 180

Query: 187 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 246
           ILATT V+ FVKY+FYV+D+LM+GQWE+KPVYTFYLELIRDLLHLSMY+CFF VIFM YG
Sbjct: 181 ILATTTVAIFVKYVFYVTDMLMDGQWEKKPVYTFYLELIRDLLHLSMYICFFFVIFMNYG 240

Query: 247 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 306
           VPLHL+RELYE FRNF+IR++DY+RYRKITSNMNDRFPDATPEEL +SDATCIICREEMT
Sbjct: 241 VPLHLLRELYETFRNFQIRVSDYLRYRKITSNMNDRFPDATPEELTASDATCIICREEMT 300

Query: 307 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQGAGTT 366
            AKKL+CGHLFHVHCLRSWLERQ TCPTCRALVVPPE +++AA         +QQ  GT+
Sbjct: 301 NAKKLICGHLFHVHCLRSWLERQQTCPTCRALVVPPENATSAAPGQRELHQGSQQ--GTS 360

Query: 367 SSSAQG---TSDTGVANDNLSHHQARVRAAVAAASVYEKSYVYPCASTLVWSPGYAVVPQ 426
           SS  QG   +S  GV+N++LS H AR++AA +AASVY KS VYP A+T+ WS G   VP 
Sbjct: 361 SSGNQGSEISSSAGVSNNSLSRHHARLQAAASAASVYGKSMVYPSANTVAWSSG---VPG 420

Query: 427 VERPLPDSNNIEPNGRPQQFSFTGGPSNLSFTQFPQNLFVPFQPREDTGNSTDGLGSSQN 486
            E+   + +   P                       NL V            +   +  N
Sbjct: 421 TEQVSTEPDQTLPQ---------------------HNLPV------------ENSHAYAN 480

Query: 487 VSGLHLEAQQRFLQQQIEILQNQLQHL--RKPDVEESSGAGEADKKGKSVA 533
           +S   LE  ++ L+  +EIL+N+L  L  RKP+      AGE + KGKSVA
Sbjct: 481 MSETKLEEMRKSLETHLEILRNRLHFLETRKPE-----SAGEPENKGKSVA 488

BLAST of Cp4.1LG14g05000 vs. ExPASy Swiss-Prot
Match: Q5Z880 (ERAD-associated E3 ubiquitin-protein ligase HRD1 OS=Oryza sativa subsp. japonica OX=39947 GN=HRD1 PE=2 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 9.7e-159
Identity = 305/503 (60.64%), Postives = 371/503 (73.76%), Query Frame = 0

Query: 7   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 66
           M++LQTYA  S +AT   +Y+AF+SR QFYPA+VYLSTSKI  VLLLN GLV MC+ WQL
Sbjct: 1   MIRLQTYAAFSLMATATAVYYAFSSREQFYPAMVYLSTSKICFVLLLNTGLVAMCVAWQL 60

Query: 67  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 126
            K++FLG+LREAEVERLNEQ+W+E++EILFA+TIFRQDFSVSFL MV ALLL+KALHWLA
Sbjct: 61  VKRLFLGTLREAEVERLNEQAWREVVEILFAVTIFRQDFSVSFLAMVAALLLVKALHWLA 120

Query: 127 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 186
           QKRVEY+ETTPSVP+LSH RIVSF+ FL V+D LF  NS+ SLI  R+ASV++FFSFEYM
Sbjct: 121 QKRVEYIETTPSVPMLSHARIVSFMLFLLVVDCLFLSNSLRSLIHKREASVAIFFSFEYM 180

Query: 187 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 246
           ILAT+ VSTFVKYIFYVSD+LMEGQWE+K VYTFYLELI DL+HLS+Y+ FF+ IF+ YG
Sbjct: 181 ILATSTVSTFVKYIFYVSDMLMEGQWEKKAVYTFYLELISDLVHLSLYMLFFIAIFLNYG 240

Query: 247 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 306
           VPLHLIRELYE FRNFRIRIADY+RYRKITSNMN+RFPDAT +ELN+SDATCIICREEMT
Sbjct: 241 VPLHLIRELYETFRNFRIRIADYVRYRKITSNMNERFPDATADELNASDATCIICREEMT 300

Query: 307 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQ-QGAGT 366
            AKKL+CGHLFHVHCLRSWLERQHTCPTCRA ++PP+   TAA  HG         G GT
Sbjct: 301 TAKKLLCGHLFHVHCLRSWLERQHTCPTCRAPILPPDNGRTAARPHGVHPGVQPVPGNGT 360

Query: 367 TSSSAQGTSDTGVANDNLSHHQARVRAAVAAASVYEKSYVYPCASTLVWSPGYAVVPQVE 426
             S          A +N+S  QA++ AA +AAS+Y +S+ YP A+ L     Y+  PQ  
Sbjct: 361 PGSER-------AAGENISRRQAKLEAAASAASLYGRSFAYPPANNL---NRYSTPPQST 420

Query: 427 RPLPDSNNIEPNGRPQQFSFTGGPSNLSFTQFPQNLFVPFQPREDTGNSTDGLGSSQNVS 486
              P S     + +  +   T  PS  +F                   +   + +++ + 
Sbjct: 421 SNGPQSGEASTSNQSPKGHATADPSAPTFY---------------ARGAVSSVTTTRELE 478

Query: 487 GLHLEAQQRFLQQQIEILQNQLQ 509
               +A +  ++ QIE+LQ QLQ
Sbjct: 481 SSLQKAYENAIKSQIEMLQIQLQ 478

BLAST of Cp4.1LG14g05000 vs. ExPASy Swiss-Prot
Match: Q803I8 (E3 ubiquitin-protein ligase synoviolin OS=Danio rerio OX=7955 GN=syvn1 PE=2 SV=2)

HSP 1 Score: 263.8 bits (673), Expect = 4.2e-69
Identity = 141/331 (42.60%), Postives = 201/331 (60.73%), Query Frame = 0

Query: 7   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 66
           M++       S   T  V+ HA+  + QFYP +VYL+ S  ++ +L     V++ +L +L
Sbjct: 1   MVRAALVTATSLALTGAVVAHAYFLKHQFYPTVVYLTKSSPSMAVLYIQAFVLVFLLGKL 60

Query: 67  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 126
            +K+F G LR AE+E L E+SW  + E   A T+FR DFS  F+ + T LL +K  HWLA
Sbjct: 61  MRKVFFGQLRAAEMEHLIERSWYAVTETCLAFTVFRDDFSPRFVALFTLLLFLKCFHWLA 120

Query: 127 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 186
           + RV++ME +P++  + H R++S +  L V+D LF  ++  S+I TR ASV L F FEY 
Sbjct: 121 EDRVDFMERSPNISWVFHFRVLSLMVLLGVMDFLFVNHACHSII-TRGASVQLVFGFEYA 180

Query: 187 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 246
           IL T  ++TF+KY  +  D+  E  W+ K VY  Y EL    + + +Y+ F  ++   + 
Sbjct: 181 ILMTMVLTTFIKYTLHTIDLQSENPWDNKAVYMLYTELFTGFIKVLLYMAFMTIMIKVHT 240

Query: 247 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEM- 306
            PL  IR +Y   R F+  + D I  R+   NMN  +PDATPE+L ++D  CIICREEM 
Sbjct: 241 FPLFAIRPMYLAMRQFKKAVTDAIMSRRAIRNMNTLYPDATPEDLQATDNVCIICREEMV 300

Query: 307 TVAKKLVCGHLFHVHCLRSWLERQHTCPTCR 337
           T AKKL C H+FH  CLRSW +RQ TCPTCR
Sbjct: 301 TGAKKLPCNHIFHSSCLRSWFQRQQTCPTCR 330

BLAST of Cp4.1LG14g05000 vs. ExPASy Swiss-Prot
Match: Q86TM6 (E3 ubiquitin-protein ligase synoviolin OS=Homo sapiens OX=9606 GN=SYVN1 PE=1 SV=2)

HSP 1 Score: 261.5 bits (667), Expect = 2.1e-68
Identity = 141/331 (42.60%), Postives = 198/331 (59.82%), Query Frame = 0

Query: 7   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 66
           M +       S   T  V+ HA+  + QFYP +VYL+ S  ++ +L     V++ +L ++
Sbjct: 1   MFRTAVMMAASLALTGAVVAHAYYLKHQFYPTVVYLTKSSPSMAVLYIQAFVLVFLLGKV 60

Query: 67  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 126
             K+F G LR AE+E L E+SW  + E   A T+FR DFS  F+ + T LL +K  HWLA
Sbjct: 61  MGKVFFGQLRAAEMEHLLERSWYAVTETCLAFTVFRDDFSPRFVALFTLLLFLKCFHWLA 120

Query: 127 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 186
           + RV++ME +P++  L H RIVS +  L +LD LF  ++  S++ TR ASV L F FEY 
Sbjct: 121 EDRVDFMERSPNISWLFHCRIVSLMFLLGILDFLFVSHAYHSIL-TRGASVQLVFGFEYA 180

Query: 187 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 246
           IL T  ++ F+KY+ +  D+  E  W+ K VY  Y EL    + + +Y+ F  ++   + 
Sbjct: 181 ILMTMVLTIFIKYVLHSVDLQSENPWDNKAVYMLYTELFTGFIKVLLYMAFMTIMIKVHT 240

Query: 247 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEM- 306
            PL  IR +Y   R F+  + D I  R+   NMN  +PDATPEEL + D  CIICREEM 
Sbjct: 241 FPLFAIRPMYLAMRQFKKAVTDAIMSRRAIRNMNTLYPDATPEELQAMDNVCIICREEMV 300

Query: 307 TVAKKLVCGHLFHVHCLRSWLERQHTCPTCR 337
           T AK+L C H+FH  CLRSW +RQ TCPTCR
Sbjct: 301 TGAKRLPCNHIFHTSCLRSWFQRQQTCPTCR 330

BLAST of Cp4.1LG14g05000 vs. NCBI nr
Match: XP_023553481.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita pepo subsp. pepo] >XP_023553482.1 ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1033 bits (2670), Expect = 0.0
Identity = 539/539 (100.00%), Postives = 539/539 (100.00%), Query Frame = 0

Query: 7   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 66
           MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 67  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 126
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 120

Query: 127 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 186
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180

Query: 187 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 246
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 247 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 306
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 307 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQGAGTT 366
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQGAGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQGAGTT 360

Query: 367 SSSAQGTSDTGVANDNLSHHQARVRAAVAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 426
           SSSAQGTSDTGVANDNLSHHQARVRAAVAAASVYEKSYVYPCASTLVWSPGYAVVPQVER
Sbjct: 361 SSSAQGTSDTGVANDNLSHHQARVRAAVAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 420

Query: 427 PLPDSNNIEPNGRPQQFSFTGGPSNLSFTQFPQNLFVPFQPREDTGNSTDGLGSSQNVSG 486
           PLPDSNNIEPNGRPQQFSFTGGPSNLSFTQFPQNLFVPFQPREDTGNSTDGLGSSQNVSG
Sbjct: 421 PLPDSNNIEPNGRPQQFSFTGGPSNLSFTQFPQNLFVPFQPREDTGNSTDGLGSSQNVSG 480

Query: 487 LHLEAQQRFLQQQIEILQNQLQHLRKPDVEESSGAGEADKKGKSVAFSLSPSPSSSDSR 545
           LHLEAQQRFLQQQIEILQNQLQHLRKPDVEESSGAGEADKKGKSVAFSLSPSPSSSDSR
Sbjct: 481 LHLEAQQRFLQQQIEILQNQLQHLRKPDVEESSGAGEADKKGKSVAFSLSPSPSSSDSR 539

BLAST of Cp4.1LG14g05000 vs. NCBI nr
Match: KAG6576867.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1026 bits (2653), Expect = 0.0
Identity = 538/546 (98.53%), Postives = 540/546 (98.90%), Query Frame = 0

Query: 1   MAVQSRMMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIM 60
           MAVQSRMMKLQTYAGISFIAT+VVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIM
Sbjct: 1   MAVQSRMMKLQTYAGISFIATVVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIM 60

Query: 61  CILWQLTKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIK 120
           CILWQLTKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIK
Sbjct: 61  CILWQLTKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIK 120

Query: 121 ALHWLAQKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLF 180
           ALHWLAQKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLF
Sbjct: 121 ALHWLAQKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLF 180

Query: 181 FSFEYMILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLV 240
           FSFEYMILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLV
Sbjct: 181 FSFEYMILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLV 240

Query: 241 IFMTYGVPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCII 300
           IFMTYGVPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCII
Sbjct: 241 IFMTYGVPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCII 300

Query: 301 CREEMTVAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQ 360
           CREEMTVAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQ
Sbjct: 301 CREEMTVAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQ 360

Query: 361 QGAGTTSSSAQGTSDTGVANDNLSHHQARVRAAVAAASVYEKSYVYPCASTLVWSPGYAV 420
           QG GTTSSSAQGTSDTGVANDNLSHHQARVRAA AAASVYEKSYVYPCASTLVWSPGYAV
Sbjct: 361 QGTGTTSSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAV 420

Query: 421 VPQVERPLPDSNNIEPNGRPQQFSFTGGPSNLSFTQFPQNLFVPFQPREDTGNSTDGLGS 480
           VPQVERPLPDSNNIEPNG PQQFSFTGGPSNLSFTQFPQNLFVPFQPRE TGNSTDGLGS
Sbjct: 421 VPQVERPLPDSNNIEPNGHPQQFSFTGGPSNLSFTQFPQNLFVPFQPREGTGNSTDGLGS 480

Query: 481 SQNVSGLHLEAQQRFLQQQIEILQNQLQHLRKPDVEESSGAGEADKKGKSVAFSL-SPSP 540
           SQNVSGLHLEAQQRFLQQQIEILQNQLQHLRKP+VEESSGAGEADKKGKSVAFS  SPSP
Sbjct: 481 SQNVSGLHLEAQQRFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVAFSSPSPSP 540

Query: 541 SSSDSR 545
           SSSDSR
Sbjct: 541 SSSDSR 546

BLAST of Cp4.1LG14g05000 vs. NCBI nr
Match: XP_022922503.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita moschata] >XP_022922504.1 ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita moschata])

HSP 1 Score: 1012 bits (2616), Expect = 0.0
Identity = 531/540 (98.33%), Postives = 532/540 (98.52%), Query Frame = 0

Query: 7   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 66
           MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 67  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 126
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 120

Query: 127 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 186
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180

Query: 187 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 246
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 247 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 306
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 307 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQGAGTT 366
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEG STAAGHHGPQSDANQQG GTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGLSTAAGHHGPQSDANQQGTGTT 360

Query: 367 SSSAQGTSDTGVANDNLSHHQARVRAAVAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 426
           SSSAQGTSDTGVANDNLSHHQARVRAA AAASVYEKSYVYPCASTLVWSPGYAVVPQVER
Sbjct: 361 SSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 420

Query: 427 PLPDSNNIEPNGRPQQFSFTGGPSNLSFTQFPQNLFVPFQPREDTGNSTDGLGSSQNVSG 486
           PLPDSNNIEPNG PQQFSFTGGPSNL FTQFPQNLFVPFQPRE TGNSTDGLGSSQNVSG
Sbjct: 421 PLPDSNNIEPNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLGSSQNVSG 480

Query: 487 LHLEAQQRFLQQQIEILQNQLQHLRKPDVEESSGAGEADKKGKSVAFSL-SPSPSSSDSR 545
           LHLEAQQRFLQQQIEILQNQLQHLRKP+VEESSGAGEADKKGKSVAFS  SPSPSSSDSR
Sbjct: 481 LHLEAQQRFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVAFSSPSPSPSSSDSR 540

BLAST of Cp4.1LG14g05000 vs. NCBI nr
Match: XP_022984215.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita maxima] >XP_022984216.1 ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita maxima])

HSP 1 Score: 1009 bits (2610), Expect = 0.0
Identity = 529/543 (97.42%), Postives = 533/543 (98.16%), Query Frame = 0

Query: 7   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 66
           MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGL+IMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLIIMCILWQL 60

Query: 67  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 126
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 120

Query: 127 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 186
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180

Query: 187 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 246
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 247 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 306
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDAT EELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATSEELNSSDATCIICREEMT 300

Query: 307 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQGAGTT 366
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQG GTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQGTGTT 360

Query: 367 SSSAQGTSDTGVANDNLSHHQARVRAAVAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 426
           SSSAQGTSDTGVANDNLSHHQARVRAA AAASVYEKSYVYPCASTLVWSPGYAVVPQVER
Sbjct: 361 SSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 420

Query: 427 PLPDSNNIEPNGRPQQFSFTGGPSNLSFTQFPQNLFVPFQPREDTGNSTDGLGSSQNVSG 486
           PLPDSNNIEPNG PQQFSFTGGPSNLSFTQFPQNLFVPFQPRE TGN+TDGLGSSQNVSG
Sbjct: 421 PLPDSNNIEPNGHPQQFSFTGGPSNLSFTQFPQNLFVPFQPREGTGNNTDGLGSSQNVSG 480

Query: 487 LHLEAQQRFLQQQIEILQNQLQHLRKPDVEESSGAGEADKKGKSVAFSL----SPSPSSS 545
           LHLEAQ+RFLQQQIEILQNQLQHLRKP+VEESSGAGEADKKGKSVAFS     SPSPSSS
Sbjct: 481 LHLEAQERFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVAFSSLSSPSPSPSSS 540

BLAST of Cp4.1LG14g05000 vs. NCBI nr
Match: XP_038889876.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Benincasa hispida] >XP_038889877.1 ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Benincasa hispida])

HSP 1 Score: 945 bits (2443), Expect = 0.0
Identity = 503/542 (92.80%), Postives = 511/542 (94.28%), Query Frame = 0

Query: 7   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 66
           MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 67  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 126
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSV+FLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 127 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 186
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180

Query: 187 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 246
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 247 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 306
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 307 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQGAGTT 366
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTA GHHG QSDANQQG GTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHHGVQSDANQQGTGTT 360

Query: 367 SSSAQGTSDTGVANDNLSHHQARVRAAVAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 426
           SSSAQGTS TGV NDNLSHHQARVRAA AAAS+YEKSYVYPC STLVWS GYAVVPQVER
Sbjct: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCPSTLVWSLGYAVVPQVER 420

Query: 427 PLPDSNNIE------PNGRPQQFSFTGGPSNLSFTQFPQNLFVPFQPREDTGNSTDGLGS 486
           PLPDS+N E      PNG PQ FSF  GPSNLSFTQFPQNLFVPFQPRE TGN+TD LGS
Sbjct: 421 PLPDSDNTETNGEQTPNGHPQYFSFPSGPSNLSFTQFPQNLFVPFQPREGTGNNTDELGS 480

Query: 487 SQNVSGLHLEAQQRFLQQQIEILQNQLQHLRKPDVEESSGAGEADKKGKSVAFSLSPSPS 542
           S NVSG  LEAQ++FLQQ IEILQNQLQ LR+P VEESS AGEADKKGKS+A S SP PS
Sbjct: 481 SLNVSGSQLEAQEKFLQQ-IEILQNQLQLLRRPKVEESSRAGEADKKGKSLATSSSPLPS 540

BLAST of Cp4.1LG14g05000 vs. ExPASy TrEMBL
Match: A0A6J1E3K4 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucurbita moschata OX=3662 GN=LOC111430487 PE=4 SV=1)

HSP 1 Score: 1012 bits (2616), Expect = 0.0
Identity = 531/540 (98.33%), Postives = 532/540 (98.52%), Query Frame = 0

Query: 7   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 66
           MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 67  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 126
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 120

Query: 127 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 186
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180

Query: 187 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 246
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 247 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 306
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 307 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQGAGTT 366
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEG STAAGHHGPQSDANQQG GTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGLSTAAGHHGPQSDANQQGTGTT 360

Query: 367 SSSAQGTSDTGVANDNLSHHQARVRAAVAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 426
           SSSAQGTSDTGVANDNLSHHQARVRAA AAASVYEKSYVYPCASTLVWSPGYAVVPQVER
Sbjct: 361 SSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 420

Query: 427 PLPDSNNIEPNGRPQQFSFTGGPSNLSFTQFPQNLFVPFQPREDTGNSTDGLGSSQNVSG 486
           PLPDSNNIEPNG PQQFSFTGGPSNL FTQFPQNLFVPFQPRE TGNSTDGLGSSQNVSG
Sbjct: 421 PLPDSNNIEPNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLGSSQNVSG 480

Query: 487 LHLEAQQRFLQQQIEILQNQLQHLRKPDVEESSGAGEADKKGKSVAFSL-SPSPSSSDSR 545
           LHLEAQQRFLQQQIEILQNQLQHLRKP+VEESSGAGEADKKGKSVAFS  SPSPSSSDSR
Sbjct: 481 LHLEAQQRFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVAFSSPSPSPSSSDSR 540

BLAST of Cp4.1LG14g05000 vs. ExPASy TrEMBL
Match: A0A6J1J826 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucurbita maxima OX=3661 GN=LOC111482600 PE=4 SV=1)

HSP 1 Score: 1009 bits (2610), Expect = 0.0
Identity = 529/543 (97.42%), Postives = 533/543 (98.16%), Query Frame = 0

Query: 7   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 66
           MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGL+IMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLIIMCILWQL 60

Query: 67  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 126
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 120

Query: 127 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 186
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180

Query: 187 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 246
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 247 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 306
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDAT EELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATSEELNSSDATCIICREEMT 300

Query: 307 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQGAGTT 366
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQG GTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQGTGTT 360

Query: 367 SSSAQGTSDTGVANDNLSHHQARVRAAVAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 426
           SSSAQGTSDTGVANDNLSHHQARVRAA AAASVYEKSYVYPCASTLVWSPGYAVVPQVER
Sbjct: 361 SSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 420

Query: 427 PLPDSNNIEPNGRPQQFSFTGGPSNLSFTQFPQNLFVPFQPREDTGNSTDGLGSSQNVSG 486
           PLPDSNNIEPNG PQQFSFTGGPSNLSFTQFPQNLFVPFQPRE TGN+TDGLGSSQNVSG
Sbjct: 421 PLPDSNNIEPNGHPQQFSFTGGPSNLSFTQFPQNLFVPFQPREGTGNNTDGLGSSQNVSG 480

Query: 487 LHLEAQQRFLQQQIEILQNQLQHLRKPDVEESSGAGEADKKGKSVAFSL----SPSPSSS 545
           LHLEAQ+RFLQQQIEILQNQLQHLRKP+VEESSGAGEADKKGKSVAFS     SPSPSSS
Sbjct: 481 LHLEAQERFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVAFSSLSSPSPSPSSS 540

BLAST of Cp4.1LG14g05000 vs. ExPASy TrEMBL
Match: A0A5A7V9K3 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G001010 PE=4 SV=1)

HSP 1 Score: 936 bits (2420), Expect = 0.0
Identity = 498/545 (91.38%), Postives = 510/545 (93.58%), Query Frame = 0

Query: 7   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 66
           MMKLQTYAGISFIATI+VIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 67  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 126
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSV+FLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 127 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 186
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSI+SLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180

Query: 187 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 246
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 247 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 306
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 307 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQGAGTT 366
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSST +GHHG QSDANQQG GTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360

Query: 367 SSSAQGTSDTGVANDNLSHHQARVRAAVAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 426
           SSSAQGTS TGV NDNLSHHQARVRAA AAAS+YEKSYVYPCASTLVWS GYAVVPQVER
Sbjct: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420

Query: 427 PLPDSNNIE------PNGRPQQFSFTGGPSNLSFTQFPQNLFVPFQPREDTGNSTDGLGS 486
           PL DSNN E      PNG PQ FSF GGPSNLSFTQFPQN+FVPFQPRE TGN+ + LGS
Sbjct: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480

Query: 487 SQNVSGLHLEAQQRFLQQQIEILQNQLQHLRKPDVEESS-GAGEADKKGKSVAFSLSPSP 544
           S NVSG  LEAQ++FLQQQIE LQNQLQ LRKP VEESS GA E DKKGKS+A S +PS 
Sbjct: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASETDKKGKSLAISPAPSA 540

BLAST of Cp4.1LG14g05000 vs. ExPASy TrEMBL
Match: A0A0A0LJG4 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G013330 PE=4 SV=1)

HSP 1 Score: 936 bits (2418), Expect = 0.0
Identity = 499/545 (91.56%), Postives = 509/545 (93.39%), Query Frame = 0

Query: 7   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 66
           MMKLQTYAGISFIATI+VIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 67  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 126
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSV+FLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 127 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 186
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSI+SLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180

Query: 187 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 246
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 247 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 306
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 307 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQGAGTT 366
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSST  GHHG QSDANQQG GTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTTGHHGVQSDANQQGTGTT 360

Query: 367 SSSAQGTSDTGVANDNLSHHQARVRAAVAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 426
           SSSAQGTS TGV NDNLSHHQARVRAA AAAS+YEKSYVYPCASTLVWS GYAVVPQVER
Sbjct: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420

Query: 427 PLPDSNNIE------PNGRPQQFSFTGGPSNLSFTQFPQNLFVPFQPREDTGNSTDGLGS 486
           PL DSNN E      PNG PQ FSF GGPSNLSFTQFPQN+FVPFQPRE TGN+ + LGS
Sbjct: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480

Query: 487 SQNVSGLHLEAQQRFLQQQIEILQNQLQHLRKPDVEESS-GAGEADKKGKSVAFSLSPSP 544
           S NVSG  LEAQ++FLQQQIE LQNQLQ LRKP VEESS GA EADKKGKS+A S S S 
Sbjct: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLAISPSSSA 540

BLAST of Cp4.1LG14g05000 vs. ExPASy TrEMBL
Match: A0A1S4E231 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucumis melo OX=3656 GN=LOC103495570 PE=4 SV=1)

HSP 1 Score: 934 bits (2415), Expect = 0.0
Identity = 498/545 (91.38%), Postives = 509/545 (93.39%), Query Frame = 0

Query: 7   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 66
           MMKLQTYAGISFIATI+VIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 67  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 126
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSV+FLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 127 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 186
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSI+SLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180

Query: 187 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 246
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 247 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 306
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 307 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQGAGTT 366
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSST +GHHG QSDANQQG GTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360

Query: 367 SSSAQGTSDTGVANDNLSHHQARVRAAVAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 426
           SSSAQGTS TGV N NLSHHQARVRAA AAAS+YEKSYVYPCASTLVWS GYAVVPQVER
Sbjct: 361 SSSAQGTSGTGVTNANLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420

Query: 427 PLPDSNNIE------PNGRPQQFSFTGGPSNLSFTQFPQNLFVPFQPREDTGNSTDGLGS 486
           PL DSNN E      PNG PQ FSF GGPSNLSFTQFPQN+FVPFQPRE TGN+ + LGS
Sbjct: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480

Query: 487 SQNVSGLHLEAQQRFLQQQIEILQNQLQHLRKPDVEESS-GAGEADKKGKSVAFSLSPSP 544
           S NVSG  LEAQ++FLQQQIE LQNQLQ LRKP VEESS GA E DKKGKS+A S SPS 
Sbjct: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASETDKKGKSLAISPSPSA 540

BLAST of Cp4.1LG14g05000 vs. TAIR 10
Match: AT1G65040.2 (RING/U-box superfamily protein )

HSP 1 Score: 615.1 bits (1585), Expect = 5.2e-176
Identity = 335/528 (63.45%), Postives = 396/528 (75.00%), Query Frame = 0

Query: 7   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 66
           M++L+ YAG+S +AT+VVIYHAF+SRGQFYPA VYLSTSKINLV+LLNMGLV+M  LW L
Sbjct: 1   MIQLKVYAGLSTLATLVVIYHAFSSRGQFYPATVYLSTSKINLVVLLNMGLVLMLSLWNL 60

Query: 67  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 126
            K +FLGSLREAEVERLNEQ+W+ELMEILFAITIFRQDFSV F+ +V  LLLIK LHW+A
Sbjct: 61  VKIVFLGSLREAEVERLNEQAWRELMEILFAITIFRQDFSVGFISLVVTLLLIKGLHWMA 120

Query: 127 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 186
           QKRVEY+ETTPSV LLSH+RIVSF+ FL +LD L  Y+SI+ LIQ+RKAS+S+FF+FEYM
Sbjct: 121 QKRVEYIETTPSVTLLSHVRIVSFMVFLLILDCLLTYSSIQQLIQSRKASMSVFFTFEYM 180

Query: 187 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 246
           ILATT VS  VKY FYV+D+L EGQWE KPVYTFYLEL+RDLLHLSMYLCFFL+IFM YG
Sbjct: 181 ILATTTVSIIVKYAFYVTDMLKEGQWEGKPVYTFYLELVRDLLHLSMYLCFFLMIFMNYG 240

Query: 247 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 306
           +PLHLIRELYE FRNF+IR+ DY+RYRKITSNMNDRFPDATPEEL+S+DATCIICREEMT
Sbjct: 241 LPLHLIRELYETFRNFKIRVTDYLRYRKITSNMNDRFPDATPEELSSNDATCIICREEMT 300

Query: 307 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPE-GSSTAAGHHGPQSDANQQGAGT 366
            AKKLVCGHLFHVHCLRSWLERQ+TCPTCRALVVP E  +STA+G+ GP  ++ QQG GT
Sbjct: 301 SAKKLVCGHLFHVHCLRSWLERQNTCPTCRALVVPAENATSTASGNRGPHQESLQQGTGT 360

Query: 367 TSSSAQGTSDTGVANDNLSHHQARVRAAVAAASVYEKSYVYP-CASTLVWSPGYAVVPQV 426
           +SS  QG+S +  A++N+S H+AR +AA +AAS+Y +S VYP  A+TLVWS G +++PQ 
Sbjct: 361 SSSDGQGSSVSAAASENMSRHEARFQAAASAASIYGRSIVYPSSANTLVWSQGNSLLPQT 420

Query: 427 ERPLPDSNNIEPNGRPQQFSFTGGPSNLSFTQFPQNLFVPFQPREDTGNSTDGLGSSQNV 486
           E                                                           
Sbjct: 421 E----------------------------------------------------------- 457

Query: 487 SGLHLEAQQRFLQQQIEILQNQLQHLRKPDVEESSGAGEADKKGKSVA 533
               +EAQ+RFL+ QIE+L NQL+ L KP           D KGKSVA
Sbjct: 481 ----VEAQRRFLESQIEVLTNQLRLLEKPTT--------VDTKGKSVA 457

BLAST of Cp4.1LG14g05000 vs. TAIR 10
Match: AT3G16090.1 (RING/U-box superfamily protein )

HSP 1 Score: 609.8 bits (1571), Expect = 2.2e-174
Identity = 341/531 (64.22%), Postives = 403/531 (75.89%), Query Frame = 0

Query: 7   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 66
           M++L+TYAG+SF+AT+ VIYHAF+SRGQFYPA VYLSTSKI+LVLLLNM LV+M  LW L
Sbjct: 1   MIRLRTYAGLSFMATLAVIYHAFSSRGQFYPATVYLSTSKISLVLLLNMCLVLMLSLWHL 60

Query: 67  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 126
            K +FLGSLREAEVERLNEQ+W+ELMEILFAITIFRQDFS  FL +V  LLLIKALHWLA
Sbjct: 61  VKFVFLGSLREAEVERLNEQAWRELMEILFAITIFRQDFSSGFLPLVVTLLLIKALHWLA 120

Query: 127 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 186
           QKRVEY+ETTPSV  LSH RIVSF+GFL ++DSLF Y+SI  LIQ+R+ASVSLFFSFEYM
Sbjct: 121 QKRVEYIETTPSVSKLSHFRIVSFMGFLLLVDSLFMYSSIRHLIQSRQASVSLFFSFEYM 180

Query: 187 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 246
           ILATT V+ FVKY+FYV+D+LM+GQWE+KPVYTFYLELIRDLLHLSMY+CFF VIFM YG
Sbjct: 181 ILATTTVAIFVKYVFYVTDMLMDGQWEKKPVYTFYLELIRDLLHLSMYICFFFVIFMNYG 240

Query: 247 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 306
           VPLHL+RELYE FRNF+IR++DY+RYRKITSNMNDRFPDATPEEL +SDATCIICREEMT
Sbjct: 241 VPLHLLRELYETFRNFQIRVSDYLRYRKITSNMNDRFPDATPEELTASDATCIICREEMT 300

Query: 307 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQGAGTT 366
            AKKL+CGHLFHVHCLRSWLERQ TCPTCRALVVPPE +++AA         +QQ  GT+
Sbjct: 301 NAKKLICGHLFHVHCLRSWLERQQTCPTCRALVVPPENATSAAPGQRELHQGSQQ--GTS 360

Query: 367 SSSAQG---TSDTGVANDNLSHHQARVRAAVAAASVYEKSYVYPCASTLVWSPGYAVVPQ 426
           SS  QG   +S  GV+N++LS H AR++AA +AASVY KS VYP A+T+ WS G   VP 
Sbjct: 361 SSGNQGSEISSSAGVSNNSLSRHHARLQAAASAASVYGKSMVYPSANTVAWSSG---VPG 420

Query: 427 VERPLPDSNNIEPNGRPQQFSFTGGPSNLSFTQFPQNLFVPFQPREDTGNSTDGLGSSQN 486
            E+   + +   P                       NL V            +   +  N
Sbjct: 421 TEQVSTEPDQTLPQ---------------------HNLPV------------ENSHAYAN 480

Query: 487 VSGLHLEAQQRFLQQQIEILQNQLQHL--RKPDVEESSGAGEADKKGKSVA 533
           +S   LE  ++ L+  +EIL+N+L  L  RKP+      AGE + KGKSVA
Sbjct: 481 MSETKLEEMRKSLETHLEILRNRLHFLETRKPE-----SAGEPENKGKSVA 488

BLAST of Cp4.1LG14g05000 vs. TAIR 10
Match: AT1G65040.3 (RING/U-box superfamily protein )

HSP 1 Score: 482.6 bits (1241), Expect = 4.1e-136
Identity = 280/528 (53.03%), Postives = 334/528 (63.26%), Query Frame = 0

Query: 7   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 66
           M++L+ YAG+S +AT+VVIYHAF+SRGQFYPA VYLSTSKINL                 
Sbjct: 1   MIQLKVYAGLSTLATLVVIYHAFSSRGQFYPATVYLSTSKINL----------------- 60

Query: 67  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 126
                                                                  LHW+A
Sbjct: 61  ------------------------------------------------------GLHWMA 120

Query: 127 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 186
           QKRVEY+ETTPSV LLSH+RIVSF+ FL +LD L  Y+SI+ LIQ+RKAS+S+FF+FEYM
Sbjct: 121 QKRVEYIETTPSVTLLSHVRIVSFMVFLLILDCLLTYSSIQQLIQSRKASMSVFFTFEYM 180

Query: 187 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 246
           ILATT VS  VKY FYV+D+L EGQWE KPVYTFYLEL+RDLLHLSMYLCFFL+IFM YG
Sbjct: 181 ILATTTVSIIVKYAFYVTDMLKEGQWEGKPVYTFYLELVRDLLHLSMYLCFFLMIFMNYG 240

Query: 247 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 306
           +PLHLIRELYE FRNF+IR+ DY+RYRKITSNMNDRFPDATPEEL+S+DATCIICREEMT
Sbjct: 241 LPLHLIRELYETFRNFKIRVTDYLRYRKITSNMNDRFPDATPEELSSNDATCIICREEMT 300

Query: 307 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPE-GSSTAAGHHGPQSDANQQGAGT 366
            AKKLVCGHLFHVHCLRSWLERQ+TCPTCRALVVP E  +STA+G+ GP  ++ QQG GT
Sbjct: 301 SAKKLVCGHLFHVHCLRSWLERQNTCPTCRALVVPAENATSTASGNRGPHQESLQQGTGT 360

Query: 367 TSSSAQGTSDTGVANDNLSHHQARVRAAVAAASVYEKSYVYP-CASTLVWSPGYAVVPQV 426
           +SS  QG+S +  A++N+S H+AR +AA +AAS+Y +S VYP  A+TLVWS G +++PQ 
Sbjct: 361 SSSDGQGSSVSAAASENMSRHEARFQAAASAASIYGRSIVYPSSANTLVWSQGNSLLPQT 386

Query: 427 ERPLPDSNNIEPNGRPQQFSFTGGPSNLSFTQFPQNLFVPFQPREDTGNSTDGLGSSQNV 486
           E                                                           
Sbjct: 421 E----------------------------------------------------------- 386

Query: 487 SGLHLEAQQRFLQQQIEILQNQLQHLRKPDVEESSGAGEADKKGKSVA 533
               +EAQ+RFL+ QIE+L NQL+ L KP           D KGKSVA
Sbjct: 481 ----VEAQRRFLESQIEVLTNQLRLLEKPTT--------VDTKGKSVA 386

BLAST of Cp4.1LG14g05000 vs. TAIR 10
Match: AT1G65040.1 (RING/U-box superfamily protein )

HSP 1 Score: 359.0 bits (920), Expect = 6.8e-99
Identity = 201/349 (57.59%), Postives = 237/349 (67.91%), Query Frame = 0

Query: 186 MILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTY 245
           MILATT VS  VKY FYV+D+L EGQWE KPVYTFYLEL+RDLLHLSMYLCFFL+IFM Y
Sbjct: 1   MILATTTVSIIVKYAFYVTDMLKEGQWEGKPVYTFYLELVRDLLHLSMYLCFFLMIFMNY 60

Query: 246 GVPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEM 305
           G+PLHLIRELYE FRNF+IR+ DY+RYRKITSNMNDRFPDATPEEL+S+DATCIICREEM
Sbjct: 61  GLPLHLIRELYETFRNFKIRVTDYLRYRKITSNMNDRFPDATPEELSSNDATCIICREEM 120

Query: 306 TVAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPE-GSSTAAGHHGPQSDANQQGAG 365
           T AKKLVCGHLFHVHCLRSWLERQ+TCPTCRALVVP E  +STA+G+ GP  ++ QQG G
Sbjct: 121 TSAKKLVCGHLFHVHCLRSWLERQNTCPTCRALVVPAENATSTASGNRGPHQESLQQGTG 180

Query: 366 TTSSSAQGTSDTGVANDNLSHHQARVRAAVAAASVYEKSYVYP-CASTLVWSPGYAVVPQ 425
           T+SS  QG+S +  A++N+S H+AR +AA +AAS+Y +S VYP  A+TLVWS G +++PQ
Sbjct: 181 TSSSDGQGSSVSAAASENMSRHEARFQAAASAASIYGRSIVYPSSANTLVWSQGNSLLPQ 240

Query: 426 VERPLPDSNNIEPNGRPQQFSFTGGPSNLSFTQFPQNLFVPFQPREDTGNSTDGLGSSQN 485
            E                                                          
Sbjct: 241 TE---------------------------------------------------------- 278

Query: 486 VSGLHLEAQQRFLQQQIEILQNQLQHLRKPDVEESSGAGEADKKGKSVA 533
                +EAQ+RFL+ QIE+L NQL+ L KP           D KGKSVA
Sbjct: 301 -----VEAQRRFLESQIEVLTNQLRLLEKPTT--------VDTKGKSVA 278

BLAST of Cp4.1LG14g05000 vs. TAIR 10
Match: AT4G25230.1 (RPM1 interacting protein 2 )

HSP 1 Score: 94.7 bits (234), Expect = 2.4e-19
Identity = 97/343 (28.28%), Postives = 153/343 (44.61%), Query Frame = 0

Query: 31  SRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQLTKKIFLGSLREAEVERLNEQSWKE 90
           S G    AL  L  S   + LL N  L +  +L    K +F G L + E ++L E+    
Sbjct: 42  SLGDSEHALELLLGSYFTIALLTNFVLNVYILLVLSLKTLFFGDLYDVETKKLVERLANY 101

Query: 91  L------MEILFAITIFRQDFSVSFLGMVTALLLIKALHWLAQKRVEYMETTPSVPLLSH 150
           +      + ++   TIF+    V +   +T L  +K    LA+ R+E +  +PS    ++
Sbjct: 102 IIYKGTFLPLVIPPTIFQ---GVLWTVWLTVLCTLKMFQALARDRLERLNASPSSTPWTY 161

Query: 151 IRIVSFLGFLFVLD------SLFFYNSIESLIQTRKASVSLFFSFE----YMILATTAVS 210
            R+ S L  +  +D      SL  YN+I S +           +FE     +I     + 
Sbjct: 162 FRVYSVLFLVLSVDMFWIKLSLMTYNTIGSAVYLLLLFEPCSIAFETLQALLIHGFQLLD 221

Query: 211 TFVKYIF----------YVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMT 270
            ++ ++           ++  +      E K +    L    D+  L M L  +L I+  
Sbjct: 222 MWINHLAVKNSDCQRSKFIDSMTAGSLLEWKGLLNRNLGFFLDMATLVMALGHYLHIWWL 281

Query: 271 YGVPLHLIRELYEIFRNFRI-------RIADYIRYRKITSNMNDRFPDATPEELNSSDAT 330
           +G+  HL+  +  +F N R        RI  YI+ R     ++   PDAT EEL + D  
Sbjct: 282 HGIAFHLVDAV--LFLNIRALLSAILKRIKGYIKLRIALGALHAALPDATSEELRAYDDE 341

Query: 331 CIICREEMTVAKKLVCGHLFHVHCLRSWLER----QHTCPTCR 337
           C ICRE M  AK+L C HLFH+ CLRSWL++     ++CPTCR
Sbjct: 342 CAICREPMAKAKRLHCNHLFHLGCLRSWLDQGLNEVYSCPTCR 379

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6NPT77.4e-17563.45ERAD-associated E3 ubiquitin-protein ligase HRD1B OS=Arabidopsis thaliana OX=370... [more]
Q9LW773.1e-17364.22ERAD-associated E3 ubiquitin-protein ligase HRD1A OS=Arabidopsis thaliana OX=370... [more]
Q5Z8809.7e-15960.64ERAD-associated E3 ubiquitin-protein ligase HRD1 OS=Oryza sativa subsp. japonica... [more]
Q803I84.2e-6942.60E3 ubiquitin-protein ligase synoviolin OS=Danio rerio OX=7955 GN=syvn1 PE=2 SV=2[more]
Q86TM62.1e-6842.60E3 ubiquitin-protein ligase synoviolin OS=Homo sapiens OX=9606 GN=SYVN1 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
XP_023553481.10.0100.00ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita pepo subsp. pe... [more]
KAG6576867.10.098.53ERAD-associated E3 ubiquitin-protein ligase HRD1A, partial [Cucurbita argyrosper... [more]
XP_022922503.10.098.33ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita moschata] >XP_... [more]
XP_022984215.10.097.42ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita maxima] >XP_02... [more]
XP_038889876.10.092.80ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Benincasa hispida] >XP_0... [more]
Match NameE-valueIdentityDescription
A0A6J1E3K40.098.33ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucurbita moschata OX=... [more]
A0A6J1J8260.097.42ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucurbita maxima OX=36... [more]
A0A5A7V9K30.091.38ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucumis melo var. maku... [more]
A0A0A0LJG40.091.56RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G013330 P... [more]
A0A1S4E2310.091.38ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucumis melo OX=3656 G... [more]
Match NameE-valueIdentityDescription
AT1G65040.25.2e-17663.45RING/U-box superfamily protein [more]
AT3G16090.12.2e-17464.22RING/U-box superfamily protein [more]
AT1G65040.34.1e-13653.03RING/U-box superfamily protein [more]
AT1G65040.16.8e-9957.59RING/U-box superfamily protein [more]
AT4G25230.12.4e-1928.28RPM1 interacting protein 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 298..335
e-value: 2.0E-5
score: 34.1
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 297..336
e-value: 1.2E-10
score: 41.6
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 298..336
score: 12.415513
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 270..342
e-value: 5.2E-23
score: 82.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 510..524
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 434..448
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 429..448
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 349..380
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 343..380
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 510..545
NoneNo IPR availablePANTHERPTHR22763RING ZINC FINGER PROTEINcoord: 7..449
NoneNo IPR availablePANTHERPTHR22763:SF174SUBFAMILY NOT NAMEDcoord: 7..449
NoneNo IPR availableCDDcd16479RING-H2_synoviolincoord: 295..336
e-value: 1.54002E-27
score: 102.376
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 278..342

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG14g05000.1Cp4.1LG14g05000.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0061630 ubiquitin protein ligase activity