Cp4.1LG14g04760 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG14g04760
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionPyruvate kinase
LocationCp4.1LG14: 1454729 .. 1459322 (+)
RNA-Seq ExpressionCp4.1LG14g04760
SyntenyCp4.1LG14g04760
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTAACAGAGCGCCAAAAGTCTTCAAGATTTACAACTATATTAGGGTTTTACGCGATAAATCTTCTAGCCGAATATTCTTCTTCTGCTTTTCTTGTAAAATCCACCATTGTTCGTTACCGCTGGTCCAAATTTGGCGGACAATACTATTCGCAGGCTATCCAAGGCCTGTTGCTGAAGAAGGACGAAGATAGCTGCTGAACTTTTTTCTTTTGTTGCTCTTTGTCAATGGCGGAGGTAAATGCTATGATGATTCGTGCTGGTTGCAATTTTGTTGCGAAAAATCCTGTATTAGATGTGAAGCGTTCGGGTTTTGGCATGCCGGTTTTCGGTCGAACAGTTTGGTGCAATCGAAGGAGGACGACGGGGAGAGTTGGAAATGGGGTGAAGGCGGTAATGCAAGTGGATTTGGAGGATGCGGAGAGATTGAGAAGATTGGAGGGCTTAGAAGCGACATTAGGGCTTGATGTTGTAGCGGAAAGGGAGTTGAAGGAGAAAGGATTTTTAGGGATGAGGAAGACGAAGCTTGTATGTACTATTGGCCCTGCGTGTAGTGCGATTGAGGATCTGGAGAAGTTGGCATTGGAAGGTATGAACATTGCGAGATTGAATATGTGCCACAATTCGAGAGAGTGGCATTGCGATGTGATTAGGAAGATCAAGAAGTTAAATGAGGGGAAAGGATTCTGTATTTCGGTGATGATCGATACTGAAGGGAGTCAGATTCATGTGGTTGATCATGGAGCTCCTTCCTCCATAAAAGTAGAGGTAAATGTTATATCTCTGTTATTTTTTGCTGCTATTTAACCGAAATATGATACTATGTTCACTGTTTTCTTCTGTAAATTAAGAACACTGAGTCTAAATTTTCTGGCCATGATTGTTCAGGATGGTTCAATCTGGTTGTTTACTGCTGAGAAATTTGAGGGCTCTCGTCCATTCACTGTTCAAGCGAGCTATGAAGGTTTCTCTGAAGGTGTGTCTGAATCTTTTGTTTCTTCGTCTTCTGGATTGTGATTAGAAGAAATATTTGAATCCCACAAGTTACTTAGAATATGAGTTTGATGTTGATGAGCTTTCTTGTATGAGTTTGGGCAGGTATTCAAGTGGGTGATTCAGTTGTAATTGATGGAGGAATGGCAACCTTTGAGGTTATTGAAAAGATTGGGAATGATTTAAGCTGCAGGTGCACAGACCCTGGTTTGTTTCTTCCTCGAGCAAAGTGCAGCTTTTGGAGAGATGGGAGGGTTGTTGTGAGAAATCACGAGTCTCCAACGCTGTCGAGTAAGGTAGGAACGTGTCTCTGCTGCATATCAATACTCATTCTGAGAAATTTAGTTGCGATGAAATATGTGAGTTGATTTTAGTGTGAGATTTGACGTTGGCCGGTGATGGAATGGGAACGAAACATTTCTTATGAGGGGTGAAAATACATTTCTTATAAGGGGTGAAAATCTTTTTCTAGTAGATACGTTTTAAAACCATGAACCATGAATCTGATGACGATATATAATGGGCCAAAACGGACAATATCTGCTAGCGGTGGATTTGGGTTGTTACGAAGGGTATTAGAGCCAGACACGTGGTGTGCTAACAAAGACGTTGGACCGCTAATGGGAGTGGATTGTGAGATTCCACATTAGTTGGAGAAGGAAACAAAACATTCCTCGTAAGGATGTAGAAACCTCTCCCTAGTAGACGCATTTTAAAACTGTGAGGTTAACGGCGATACGTAACGGGCCAAAGTGGACAATATCTACTAGCGGAGGACTTGGGCTATTACAAATGGTATCAGAGCCAAACGTCTGGTGGTGTGCCAACAAGGACGCTGGCCCCAAGGGGTGGATTCTGAGATTCCACATTGGTTGGAGAGGGGAACCAAGTATTCCTTGTAAGGGTGTGAAAACGTCTCCCTAGTAGACGTGTTTTATAACCGTGAGTCTGACGACGATACGTAACAGGCCAAAGTGAACAATATCCTCTAGCGGTGGGCTTGGATAGACACAGAATGAAGTTGAAAGTTTAAAATATGATGTATTTTATTGATTGGCCTTTTCATATTAATGCACAAACATGAGTAAAAGAACTCAATTAGTCATGTCTTCTTGGATGATGCCGGATCTCTGCGTTTAAACCCTGTTTTTTCATGGTGTATTACAGGATTGGTCGGACATTGAATTTGGCATTGCGGAAGGCGTCGACTTCATTGCCCTTTCCTTCGTGAACAGTGCTGATCCTGTCAAGCATCTAAAGAACTACCTATCAACAAAATCAGCAGAGTAAGCATCGATTCAAGAAATCAACTTTTACCATGAATTGGGTTGAACCATCACTATCACTATCACTACCACTACTATGCATGAACGATAAATCCCCATGTCCTTTTCAATAGAATTTTCATCAGCACAGCTACAATTATAGAGACATTCTTTTCCTTGAAAATTTTCTGGCAAAATATTGTTACTGAAACAGAAAATTTGGGTAATTTAAAAACTGAATTTAGTGATGAAGGGGAAAATCTGTTTTCATGCACAGATCCGTAAGGGTATTGGCTAAGATTGAAAGCTTGGAGTCTCTTCAGAATCTGGAAGAAATTATAGAGGCCTCTGATGGAATCATGGTAGCTCGAGGAGATCTTGGGGTTGAAATTCCGCTGGAGCAGATACCAGCAGTCCAAGAGGAAATAACTAGTGTTTGTAGGGAACTGAACAAACCAGTGATCATAGCTTCTCAACTTCTAGAGTCGATGGTTGAATATCCTACACCAACACGAGCTGAGGTATGAAAATTTTATTTTCGGTTTCTAATTTCAGGCATGTTTGGAACGATTTCCAAAATGATTGTTGAAGACTATAATGATGATCCATTTTGGTGGGGTGTTGAATAGGTTGCAGATGTTTCGGAGGCTGTCCGACAGTATGCTGATGCATTAATGTTATCTGGGGAGTCTGCCATAGGATCATATGGACAGAAAGCGCTTTCGGTTCTACAGATGGCCAGCTGCCGAATGGAGTTATGGAGTCGCGAAGAAAATAGGGAAAATTTCCTTCCCCACTATCAACTTGGGGTGTCATTGCAGGATCGCATTGCAGAAGAAATTTGCAATTCTGCTGCTGAATTAGGTAAAACAGTTTGGTTTTTCGCTTTTATTCAACCATTTTTTTAGAGAATATATGGTTTTTAAATTCTAAAAGATTTCTACTCCAACCTTTGTAAAAATGAAGAGTAATTTTTACAAGTACAAGAATAGCTTTTAAATGTCAATGAACCTGTGAGACTTTCCATTCGTATGGAAGACCGTTAGAGATATTTAGTTAAATTATAAGTTTAGTTCCTAAATTTTTAGGTTTGTGTTTATTGGTCGCGGGTCTTTCAAAAAATGTCTAAAAGTTCTTAAACTTTCAATTATGTATCCAGTAAGCCTTTGAACTTTAAAGAGTGACTAATATATTCATAATCTTTCCGTTGTATGTTTGTCAAGTTCATTAACTTTAGAAAGCGTCAAATATATAATTTTGTATTTAATAGGTCAGAGCATATTAAGTTTGCTGAGCTGACGGAATTAATTAGGCACAAAAGTTTAAGTTTTGAAGTTTATTGCATGTTTTTGTGGTATAAAGCTGTTGTAGAACTGATCCTTGCACTTAATTAGGTGTCAAGAAGTAAATTAATTACCTTTGAACGAAAACTACTGTTTTGTTTGAGTGTTGCTAATTGCATTTGGCCCACGTTTCCACCAAAACAAACATCTCCATGTTGTTTTTGGCAGCTAACAGGCTTTCAGTAGACGCCATATTTGTGCTCACAACGCAAGGACACATGGCATCTCTTCTATCCCGCAACCGCCCAAGGCCGCCTATATTTGCATTAACCGATGATGATAGCACAAGAATGGCTTTGAATCTGCAATGGGGAGTGTTTCCTCTGCGCATTGAGCTCTCAGAAGACATTGAAGCAAACATCTGGAGGGGCATCGAGCTTGTGAAATCAAAGGGGTTGGTGAAACAGGGGGACTCTGTGTTGGTGGTCTCTGAAATCTCTCCAGCTCGAGCTGCATCAATGGCGTCCCAATCAATCCAGCTGAAAACCATCGCGTAGGAGAAAGAGAAGAAGAGAACCAACCAATCCAAGAAACTTCAGTGATTTATGATTGCATGTACCATATGTGGAGGTTATTTATCTACTTATTATTTGTGGCCCTCTGTTTATAAAGACTACTTTGAAGCTTGGATAGGCCGTTTGTTGTTTTGTACTACTAGTAGCTAATATTTGGTCATTTGAATAACCGTACTGAAATTGGGTATTCTAGATTTATTTGGTAGAAACAAGGGACCAAGCGAGCTAGGTTACCGGGAGAAATCATCTTCTCTTGGTTTATGCTAATCGATTGAGTAGTCGTATTCAAGCTCCGGTAAAGGCTGATTGCTACTGTCCTACTATAAGAACAATAAGCCACAATGTTATAGATTGTCTTTTAAATATGATTAGTCGGTTTTCAAAACAGTTATAGGTAACTAATGAAGTTTTGAGGTTGATTTGGATAGGAGAAGACTCAATTGATTGGATCGATCGTAGCTTCGAGTTCAGGTGAAATTA

mRNA sequence

CCTAACAGAGCGCCAAAAGTCTTCAAGATTTACAACTATATTAGGGTTTTACGCGATAAATCTTCTAGCCGAATATTCTTCTTCTGCTTTTCTTGTAAAATCCACCATTGTTCGTTACCGCTGGTCCAAATTTGGCGGACAATACTATTCGCAGGCTATCCAAGGCCTGTTGCTGAAGAAGGACGAAGATAGCTGCTCTTTGTCAATGGCGGAGGTAAATGCTATGATGATTCGTGCTGGTTGCAATTTTGTTGCGAAAAATCCTGTATTAGATGTGAAGCGTTCGGGTTTTGGCATGCCGGTTTTCGGTCGAACAGTTTGGTGCAATCGAAGGAGGACGACGGGGAGAGTTGGAAATGGGGTGAAGGCGGTAATGCAAGTGGATTTGGAGGATGCGGAGAGATTGAGAAGATTGGAGGGCTTAGAAGCGACATTAGGGCTTGATGTTGTAGCGGAAAGGGAGTTGAAGGAGAAAGGATTTTTAGGGATGAGGAAGACGAAGCTTGTATGTACTATTGGCCCTGCGTGTAGTGCGATTGAGGATCTGGAGAAGTTGGCATTGGAAGGTATGAACATTGCGAGATTGAATATGTGCCACAATTCGAGAGAGTGGCATTGCGATGTGATTAGGAAGATCAAGAAGTTAAATGAGGGGAAAGGATTCTGTATTTCGGTGATGATCGATACTGAAGGGAGTCAGATTCATGTGGTTGATCATGGAGCTCCTTCCTCCATAAAAGTAGAGGATGGTTCAATCTGGTTGTTTACTGCTGAGAAATTTGAGGGCTCTCGTCCATTCACTGTTCAAGCGAGCTATGAAGGTTTCTCTGAAGGTATTCAAGTGGGTGATTCAGTTGTAATTGATGGAGGAATGGCAACCTTTGAGGTTATTGAAAAGATTGGGAATGATTTAAGCTGCAGGTGCACAGACCCTGGTTTGTTTCTTCCTCGAGCAAAGTGCAGCTTTTGGAGAGATGGGAGGGTTGTTGTGAGAAATCACGAGTCTCCAACGCTGTCGAGTAAGGATTGGTCGGACATTGAATTTGGCATTGCGGAAGGCGTCGACTTCATTGCCCTTTCCTTCGTGAACAGTGCTGATCCTGTCAAGCATCTAAAGAACTACCTATCAACAAAATCAGCAGAATCCGTAAGGGTATTGGCTAAGATTGAAAGCTTGGAGTCTCTTCAGAATCTGGAAGAAATTATAGAGGCCTCTGATGGAATCATGGTAGCTCGAGGAGATCTTGGGGTTGAAATTCCGCTGGAGCAGATACCAGCAGTCCAAGAGGAAATAACTAGTGTTTGTAGGGAACTGAACAAACCAGTGATCATAGCTTCTCAACTTCTAGAGTCGATGGTTGAATATCCTACACCAACACGAGCTGAGGTTGCAGATGTTTCGGAGGCTGTCCGACAGTATGCTGATGCATTAATGTTATCTGGGGAGTCTGCCATAGGATCATATGGACAGAAAGCGCTTTCGGTTCTACAGATGGCCAGCTGCCGAATGGAGTTATGGAGTCGCGAAGAAAATAGGGAAAATTTCCTTCCCCACTATCAACTTGGGGTGTCATTGCAGGATCGCATTGCAGAAGAAATTTGCAATTCTGCTGCTGAATTAGCTAACAGGCTTTCAGTAGACGCCATATTTGTGCTCACAACGCAAGGACACATGGCATCTCTTCTATCCCGCAACCGCCCAAGGCCGCCTATATTTGCATTAACCGATGATGATAGCACAAGAATGGCTTTGAATCTGCAATGGGGAGTGTTTCCTCTGCGCATTGAGCTCTCAGAAGACATTGAAGCAAACATCTGGAGGGGCATCGAGCTTGTGAAATCAAAGGGGTTGGTGAAACAGGGGGACTCTGTGTTGGTGGTCTCTGAAATCTCTCCAGCTCGAGCTGCATCAATGGCGTCCCAATCAATCCAGCTGAAAACCATCGCGTAGGAGAAAGAGAAGAAGAGAACCAACCAATCCAAGAAACTTCAGTGATTTATGATTGCATGTACCATATGTGGAGGTTATTTATCTACTTATTATTTGTGGCCCTCTGTTTATAAAGACTACTTTGAAGCTTGGATAGGCCGTTTGTTGTTTTGTACTACTAGTAGCTAATATTTGGTCATTTGAATAACCGTACTGAAATTGGGTATTCTAGATTTATTTGGTAGAAACAAGGGACCAAGCGAGCTAGGTTACCGGGAGAAATCATCTTCTCTTGGTTTATGCTAATCGATTGAGTAGTCGTATTCAAGCTCCGGTAAAGGCTGATTGCTACTGTCCTACTATAAGAACAATAAGCCACAATGTTATAGATTGTCTTTTAAATATGATTAGTCGGTTTTCAAAACAGTTATAGGTAACTAATGAAGTTTTGAGGTTGATTTGGATAGGAGAAGACTCAATTGATTGGATCGATCGTAGCTTCGAGTTCAGGTGAAATTA

Coding sequence (CDS)

ATGGCGGAGGTAAATGCTATGATGATTCGTGCTGGTTGCAATTTTGTTGCGAAAAATCCTGTATTAGATGTGAAGCGTTCGGGTTTTGGCATGCCGGTTTTCGGTCGAACAGTTTGGTGCAATCGAAGGAGGACGACGGGGAGAGTTGGAAATGGGGTGAAGGCGGTAATGCAAGTGGATTTGGAGGATGCGGAGAGATTGAGAAGATTGGAGGGCTTAGAAGCGACATTAGGGCTTGATGTTGTAGCGGAAAGGGAGTTGAAGGAGAAAGGATTTTTAGGGATGAGGAAGACGAAGCTTGTATGTACTATTGGCCCTGCGTGTAGTGCGATTGAGGATCTGGAGAAGTTGGCATTGGAAGGTATGAACATTGCGAGATTGAATATGTGCCACAATTCGAGAGAGTGGCATTGCGATGTGATTAGGAAGATCAAGAAGTTAAATGAGGGGAAAGGATTCTGTATTTCGGTGATGATCGATACTGAAGGGAGTCAGATTCATGTGGTTGATCATGGAGCTCCTTCCTCCATAAAAGTAGAGGATGGTTCAATCTGGTTGTTTACTGCTGAGAAATTTGAGGGCTCTCGTCCATTCACTGTTCAAGCGAGCTATGAAGGTTTCTCTGAAGGTATTCAAGTGGGTGATTCAGTTGTAATTGATGGAGGAATGGCAACCTTTGAGGTTATTGAAAAGATTGGGAATGATTTAAGCTGCAGGTGCACAGACCCTGGTTTGTTTCTTCCTCGAGCAAAGTGCAGCTTTTGGAGAGATGGGAGGGTTGTTGTGAGAAATCACGAGTCTCCAACGCTGTCGAGTAAGGATTGGTCGGACATTGAATTTGGCATTGCGGAAGGCGTCGACTTCATTGCCCTTTCCTTCGTGAACAGTGCTGATCCTGTCAAGCATCTAAAGAACTACCTATCAACAAAATCAGCAGAATCCGTAAGGGTATTGGCTAAGATTGAAAGCTTGGAGTCTCTTCAGAATCTGGAAGAAATTATAGAGGCCTCTGATGGAATCATGGTAGCTCGAGGAGATCTTGGGGTTGAAATTCCGCTGGAGCAGATACCAGCAGTCCAAGAGGAAATAACTAGTGTTTGTAGGGAACTGAACAAACCAGTGATCATAGCTTCTCAACTTCTAGAGTCGATGGTTGAATATCCTACACCAACACGAGCTGAGGTTGCAGATGTTTCGGAGGCTGTCCGACAGTATGCTGATGCATTAATGTTATCTGGGGAGTCTGCCATAGGATCATATGGACAGAAAGCGCTTTCGGTTCTACAGATGGCCAGCTGCCGAATGGAGTTATGGAGTCGCGAAGAAAATAGGGAAAATTTCCTTCCCCACTATCAACTTGGGGTGTCATTGCAGGATCGCATTGCAGAAGAAATTTGCAATTCTGCTGCTGAATTAGCTAACAGGCTTTCAGTAGACGCCATATTTGTGCTCACAACGCAAGGACACATGGCATCTCTTCTATCCCGCAACCGCCCAAGGCCGCCTATATTTGCATTAACCGATGATGATAGCACAAGAATGGCTTTGAATCTGCAATGGGGAGTGTTTCCTCTGCGCATTGAGCTCTCAGAAGACATTGAAGCAAACATCTGGAGGGGCATCGAGCTTGTGAAATCAAAGGGGTTGGTGAAACAGGGGGACTCTGTGTTGGTGGTCTCTGAAATCTCTCCAGCTCGAGCTGCATCAATGGCGTCCCAATCAATCCAGCTGAAAACCATCGCGTAG

Protein sequence

MAEVNAMMIRAGCNFVAKNPVLDVKRSGFGMPVFGRTVWCNRRRTTGRVGNGVKAVMQVDLEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVDFIALSFVNSADPVKHLKNYLSTKSAESVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLQDRIAEEICNSAAELANRLSVDAIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIA
Homology
BLAST of Cp4.1LG14g04760 vs. ExPASy Swiss-Prot
Match: Q9LIK0 (Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP1 PE=1 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 1.4e-171
Identity = 297/487 (60.99%), Postives = 372/487 (76.39%), Query Frame = 0

Query: 79  LDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHC 138
           +D V E ELKE GF   R+TKL+CTIGPA    E LE LA+ GMN+ARLNMCH +R+WH 
Sbjct: 100 VDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHR 159

Query: 139 DVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEGSRP- 198
            VIR +++LNE KGF +++M+DTEGS+IH+ D G  +S K EDG +W FT   F+ SRP 
Sbjct: 160 GVIRSVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPE 219

Query: 199 FTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCSFWRD 258
            T+  SY+GF+E ++VGD +++DGGM  FEVIEKIG D+ C CTDPGL LPRA  +FWRD
Sbjct: 220 RTISVSYDGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRD 279

Query: 259 GRVV-VRNHESPTLSSKDWSDIEFGIAEGVDFIALSFVNSADPVKHLKNYLSTKS-AESV 318
           G +V  RN   PT+SSKDW DI+FGIAEGVDFIA+SFV SA+ + HLK+YL+ +S    +
Sbjct: 280 GSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEI 339

Query: 319 RVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVI 378
            V+AKIES++SL NLEEII ASDG MVARGDLG +IPLEQ+PA Q+ I  VCR LNKPVI
Sbjct: 340 GVIAKIESIDSLTNLEEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVI 399

Query: 379 IASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRM 438
           +ASQLLESM+EYPTPTRAEVADVSEAVRQ +DALMLSGESA+G +  KAL+VL+  S R+
Sbjct: 400 VASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRI 459

Query: 439 ELWSREENRENFLPHYQLGVSLQDRIAEEICNSAAELANRLSVDAIFVLTTQGHMASLLS 498
           E W REE R   +P   +G S  D+I+EEICNSAA++AN L VDA+FV TT GHMASL+S
Sbjct: 460 ERWWREEKRHESVPLQAIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVS 519

Query: 499 RNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVKQGDS 558
           R RP  PIFA T   S R  LNLQWG+ P R+  S+D+E+N+ +   L+KS+G++K GD 
Sbjct: 520 RCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDL 579

Query: 559 VLVVSEI 563
           V+ VS++
Sbjct: 580 VIAVSDM 586

BLAST of Cp4.1LG14g04760 vs. ExPASy Swiss-Prot
Match: Q43117 (Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 1.5e-170
Identity = 292/491 (59.47%), Postives = 377/491 (76.78%), Query Frame = 0

Query: 75  ATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSR 134
           +++ +D V E ELKE GF   R+TKLVCTIGPA    E+LE LA+ GMN+AR+NMCH +R
Sbjct: 83  SSIEVDAVTETELKENGFRSTRRTKLVCTIGPATCGFEELEALAVGGMNVARINMCHGTR 142

Query: 135 EWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEG 194
           EWH  VI ++++LNE KGF +++M+DTEGS+IH+ D G  SS K EDG IW F+   ++ 
Sbjct: 143 EWHKSVIERVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAYDS 202

Query: 195 SRP-FTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCS 254
            RP  T+  +Y+GF+E ++VGD +++DGGM  FEVIEKIG D+ CRCTDPGL LPRA  +
Sbjct: 203 PRPERTINVNYDGFAEDVKVGDELLVDGGMVRFEVIEKIGPDVKCRCTDPGLLLPRANLT 262

Query: 255 FWRDGRVV-VRNHESPTLSSKDWSDIEFGIAEGVDFIALSFVNSADPVKHLKNYLSTKSA 314
           FWRDG +V  RN   PT+SSKDW DI+FGIAEGVDFIA+SFV SA+ + HLK+Y++ +S 
Sbjct: 263 FWRDGSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAISFVKSAEVINHLKSYIAARSR 322

Query: 315 ES-VRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELN 374
           +S + V+AKIES++SL+NLEEII ASDG MVARGDLG +IPLEQ+P+ Q+ I  VCR+LN
Sbjct: 323 DSDIAVIAKIESIDSLKNLEEIIRASDGAMVARGDLGAQIPLEQVPSAQQNIVQVCRQLN 382

Query: 375 KPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMA 434
           KPVI+ASQLLESM+EYPTPTRAEVADVSEAVRQ ADALMLSGESA+G Y +KAL+VL+  
Sbjct: 383 KPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRADALMLSGESAMGQYPEKALAVLRSV 442

Query: 435 SCRMELWSREENRENFLPHYQLGVSLQDRIAEEICNSAAELANRLSVDAIFVLTTQGHMA 494
           S R+E W REE     +    +G +  D I+EEICNSAA++AN L VDA+FV T  GHMA
Sbjct: 443 SVRIEKWWREEKHHEAMELPAIGSTYSDSISEEICNSAAKMANNLGVDALFVYTKDGHMA 502

Query: 495 SLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVK 554
           SLLSR RP  PIFA T   S R  LNLQWG+ P R+  ++D+E+N+ +   L+K++G++K
Sbjct: 503 SLLSRCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFADDMESNLNKTFSLLKARGMIK 562

Query: 555 QGDSVLVVSEI 563
            GD V+ VS++
Sbjct: 563 SGDLVIAVSDM 573

BLAST of Cp4.1LG14g04760 vs. ExPASy Swiss-Prot
Match: Q40545 (Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 6.7e-166
Identity = 286/491 (58.25%), Postives = 374/491 (76.17%), Query Frame = 0

Query: 75  ATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSR 134
           +++ +D V E ELKE GF   R+TKL+CTIGPA    E LE+LA  GMN+AR+NMCH +R
Sbjct: 93  SSIEVDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLERLAEGGMNVARINMCHGTR 152

Query: 135 EWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEG 194
           EWH  VI ++++LNE KGF +++M+DTEGS+IH+ D G  SS K EDG IW FT   F+ 
Sbjct: 153 EWHRMVIERLRRLNEEKGFAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWNFTVRSFDP 212

Query: 195 SRP-FTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCS 254
             P  TV  +Y+GF+E ++VGD +++DGGM  FEVIEKIG D+ C CTDPGL LPRA  +
Sbjct: 213 PLPERTVTVNYDGFAEDVKVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLT 272

Query: 255 FWRDGRVV-VRNHESPTLSSKDWSDIEFGIAEGVDFIALSFVNSADPVKHLKNYLSTKSA 314
           FWRDG++V  RN   PT+SSKDW DI+FGIAEGVDFIA+SFV SA+ +KHLK+Y+  ++ 
Sbjct: 273 FWRDGKLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVIKHLKSYIQARAR 332

Query: 315 ES-VRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELN 374
           +S + V+AKIES++SL+NLEEII+ASDG MVARGDLG +IPLEQ+P+ Q++I  +CR+LN
Sbjct: 333 DSDISVIAKIESIDSLKNLEEIIQASDGAMVARGDLGAQIPLEQVPSEQQKIVQICRQLN 392

Query: 375 KPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMA 434
           +PVI+ASQLLESM+EYP PTRAEVADVSEAVRQ  DALMLSGESA+G + +KAL+VL+  
Sbjct: 393 RPVIVASQLLESMIEYPIPTRAEVADVSEAVRQRGDALMLSGESAMGQFPEKALTVLRSV 452

Query: 435 SCRMELWSREENRENFLPHYQLGVSLQDRIAEEICNSAAELANRLSVDAIFVLTTQGHMA 494
           S R+E   RE+ R   +    +  S  D I+EEICNSAA++AN L VDA+FV T  GHMA
Sbjct: 453 SLRIERMWREQKRHEVIELPSIASSFSDSISEEICNSAAKMANNLEVDALFVYTKNGHMA 512

Query: 495 SLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVK 554
           SLLSR RP  PIFA T   S R  LNLQWG+ P R+  S+D+E+N+ +   L+K++G++K
Sbjct: 513 SLLSRCRPDCPIFAFTTTTSVRRRLNLQWGLMPFRLSFSDDMESNLNKTFSLLKARGMIK 572

Query: 555 QGDSVLVVSEI 563
            GD ++ VS++
Sbjct: 573 SGDLIIAVSDM 583

BLAST of Cp4.1LG14g04760 vs. ExPASy Swiss-Prot
Match: Q40546 (Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 4.0e-86
Identity = 185/465 (39.78%), Postives = 276/465 (59.35%), Query Frame = 0

Query: 96  RKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHCDVIRKIKKLN-EGKGFC 155
           RKTK+VCTIGP+ S+ E + KLA  GMN+ARLNM H     H   I  +K+ N + +   
Sbjct: 91  RKTKIVCTIGPSTSSREMIWKLAEAGMNVARLNMSHGDHASHQRTIDLVKEYNAQFEDKV 150

Query: 156 ISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVG 215
           I++M+DT+G +  V+    P  I +++G  + F+ ++   +   TV  +Y+ F   ++ G
Sbjct: 151 IAIMLDTKGPE--VISGDVPKPILLKEGQEFNFSIKRGVSTED-TVSVNYDDFINDVEAG 210

Query: 216 DSVVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKD 275
           D +++DGGM +  V  K  + + C   D G    R      R   V  ++   P+++ KD
Sbjct: 211 DILLVDGGMMSLAVKSKTSDIVKCEVIDGGELKSR------RHLNVRGKSATLPSITEKD 270

Query: 276 WSDIEFGIAEGVDFIALSFVNSADPVKHLKNYLSTKSAESVRVLAKIESLESLQNLEEII 335
           W DI+FG+   VDF A+SFV  A  V  LK+YL + +A+ + V+ KIES +S+ NL  II
Sbjct: 271 WDDIKFGVNNQVDFYAVSFVKDAKVVHELKDYLKSCNAD-IHVIVKIESADSIPNLHSII 330

Query: 336 EASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIIASQLLESMVEYPTPTRAE 395
            ASDG MVARGDLG E+P+E++P +QE+I   C+ + KPVI+A+ +LESM+++PTPTRAE
Sbjct: 331 SASDGAMVARGDLGAELPIEEVPLLQEDIIRRCQSMQKPVIVATNMLESMIDHPTPTRAE 390

Query: 396 VADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRMELWSREENRENFLPHYQLG 455
           V+D+S AVR+ ADA+MLSGE+A G Y  KA+ V+ + + R      E + +         
Sbjct: 391 VSDISIAVREGADAVMLSGETAHGKYPLKAVKVMHIVALR-----TESSLQKSTSSPSQS 450

Query: 456 VSLQDRIAEEICNSAAELANRLSVDAIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRM 515
            + +  + E     ++ +AN LS   I V T  G MA +LS NRP   +FA T+++  + 
Sbjct: 451 AAYKSHMGEMFAFHSSSMANTLST-PIIVFTRTGSMAIILSHNRPSSTVFAFTNNERVKQ 510

Query: 516 ALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVKQGDSVLVV 560
            L L  GV P+ +E S D E    R I+L+ SK LVK G  V +V
Sbjct: 511 RLALYHGVVPIYMEFSSDAEETFSRAIKLLLSKSLVKDGQYVTLV 539

BLAST of Cp4.1LG14g04760 vs. ExPASy Swiss-Prot
Match: Q9FLW9 (Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 4.4e-85
Identity = 184/486 (37.86%), Postives = 281/486 (57.82%), Query Frame = 0

Query: 96  RKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHCDVIRKIKKLN-EGKGFC 155
           RKTK+VCT+GP+ +  E + KLA  GMN+AR+NM H     H  VI  +K+ N + K   
Sbjct: 110 RKTKIVCTVGPSTNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNT 169

Query: 156 ISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVG 215
           I++M+DT+G ++   D   P  I ++ G  + FT E+   S P  V  +Y+ F   ++ G
Sbjct: 170 IAIMLDTKGPEVRSGD--LPQPIMLDPGQEFTFTIER-GVSTPSCVSVNYDDFVNDVEAG 229

Query: 216 DSVVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKD 275
           D +++DGGM +F V  K  + + C   D G    R      R   V  ++   P+++ KD
Sbjct: 230 DMLLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSR------RHLNVRGKSATLPSITEKD 289

Query: 276 WSDIEFGIAEGVDFIALSFVNSADPVKHLKNYLSTKSAESVRVLAKIESLESLQNLEEII 335
           W DI+FG+   VDF A+SFV  A  V  LK YL    A+ + V+ KIES +S+ NL  II
Sbjct: 290 WEDIKFGVENKVDFYAVSFVKDAQVVHELKKYLQNSGAD-IHVIVKIESADSIPNLHSII 349

Query: 336 EASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIIASQLLESMVEYPTPTRAE 395
            ASDG MVARGDLG E+P+E++P +QEEI ++CR + K VI+A+ +LESM+ +PTPTRAE
Sbjct: 350 TASDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPTRAE 409

Query: 396 VADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRMELWSREENRENFLPHYQLG 455
           V+D++ AVR+ ADA+MLSGE+A G +  KA  V+   + R E           +P   LG
Sbjct: 410 VSDIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVALRTEATITSGE----MPP-NLG 469

Query: 456 VSLQDRIAEEICNSAAELANRLSVDAIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRM 515
            + ++ ++E     A  ++N L    + V T  G MA LLS  RP   I+A T++   + 
Sbjct: 470 QAFKNHMSEMFAYHATMMSNTLGTSTV-VFTRTGFMAILLSHYRPSGTIYAFTNEKKIQQ 529

Query: 516 ALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVKQGDSVLVVSEISPARAASMASQS 575
            L L  GV P+ +E ++D E      +  +  +G+VK+G+ + +V   +     S ++ +
Sbjct: 530 RLALYQGVCPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPIWRSQSTHN 579

Query: 576 IQLKTI 581
           IQ++ +
Sbjct: 590 IQVRKV 579

BLAST of Cp4.1LG14g04760 vs. NCBI nr
Match: XP_023553283.1 (plastidial pyruvate kinase 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1139 bits (2947), Expect = 0.0
Identity = 581/581 (100.00%), Postives = 581/581 (100.00%), Query Frame = 0

Query: 1   MAEVNAMMIRAGCNFVAKNPVLDVKRSGFGMPVFGRTVWCNRRRTTGRVGNGVKAVMQVD 60
           MAEVNAMMIRAGCNFVAKNPVLDVKRSGFGMPVFGRTVWCNRRRTTGRVGNGVKAVMQVD
Sbjct: 1   MAEVNAMMIRAGCNFVAKNPVLDVKRSGFGMPVFGRTVWCNRRRTTGRVGNGVKAVMQVD 60

Query: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120
           LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE
Sbjct: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120

Query: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180
           GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE
Sbjct: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180

Query: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCRC 240
           DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCRC
Sbjct: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCRC 240

Query: 241 TDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVDFIALSFVNSADPV 300
           TDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVDFIALSFVNSADPV
Sbjct: 241 TDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVDFIALSFVNSADPV 300

Query: 301 KHLKNYLSTKSAESVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ 360
           KHLKNYLSTKSAESVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ
Sbjct: 301 KHLKNYLSTKSAESVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ 360

Query: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420
           EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY
Sbjct: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420

Query: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLQDRIAEEICNSAAELANRLSVDA 480
           GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLQDRIAEEICNSAAELANRLSVDA
Sbjct: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLQDRIAEEICNSAAELANRLSVDA 480

Query: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540
           IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG
Sbjct: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540

Query: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIA 581
           IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIA
Sbjct: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIA 581

BLAST of Cp4.1LG14g04760 vs. NCBI nr
Match: KAG7014927.1 (Plastidial pyruvate kinase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1121 bits (2900), Expect = 0.0
Identity = 573/580 (98.79%), Postives = 576/580 (99.31%), Query Frame = 0

Query: 1   MAEVNAMMIRAGCNFVAKNPVLDVKRSGFGMPVFGRTVWCNRRRTTGRVGNGVKAVMQVD 60
           MAEVNAMMIRAGCNFVAKNPVLDVKRSGFGMPVFGRTVWCNRR TTGRVGNGVKAVMQVD
Sbjct: 1   MAEVNAMMIRAGCNFVAKNPVLDVKRSGFGMPVFGRTVWCNRR-TTGRVGNGVKAVMQVD 60

Query: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120
           LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE
Sbjct: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120

Query: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180
           GMNIARLNMCHNSREWH DVIRKIKKLNEGKGFCISVMIDTEGSQ+HVVDHGAPSSIKVE
Sbjct: 121 GMNIARLNMCHNSREWHRDVIRKIKKLNEGKGFCISVMIDTEGSQVHVVDHGAPSSIKVE 180

Query: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCRC 240
           DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDS+VIDGGMATFEVIEKIGNDLSCRC
Sbjct: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMATFEVIEKIGNDLSCRC 240

Query: 241 TDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVDFIALSFVNSADPV 300
           TDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKDWSDIEFGI EGVDFIALSFVNSADPV
Sbjct: 241 TDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKDWSDIEFGIVEGVDFIALSFVNSADPV 300

Query: 301 KHLKNYLSTKSAESVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ 360
           KHLKNYLSTKSA+SVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ
Sbjct: 301 KHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ 360

Query: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420
           EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY
Sbjct: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420

Query: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLQDRIAEEICNSAAELANRLSVDA 480
           GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSL DRIAEEICNSAAELANRLSVDA
Sbjct: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSAAELANRLSVDA 480

Query: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540
           IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG
Sbjct: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540

Query: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 580
           IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI
Sbjct: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 579

BLAST of Cp4.1LG14g04760 vs. NCBI nr
Match: XP_022922736.1 (plastidial pyruvate kinase 1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1119 bits (2895), Expect = 0.0
Identity = 570/580 (98.28%), Postives = 576/580 (99.31%), Query Frame = 0

Query: 1   MAEVNAMMIRAGCNFVAKNPVLDVKRSGFGMPVFGRTVWCNRRRTTGRVGNGVKAVMQVD 60
           MAEVNAMMIRAGCNFVAKNPVLD+KRSGFGMPVFGRTVWCNRR TTGRVGNGVKAVMQVD
Sbjct: 1   MAEVNAMMIRAGCNFVAKNPVLDLKRSGFGMPVFGRTVWCNRR-TTGRVGNGVKAVMQVD 60

Query: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120
           LEDAERLRRLEGLEATLGLD+VAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE
Sbjct: 61  LEDAERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120

Query: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180
           GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE
Sbjct: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180

Query: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCRC 240
           DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDS+VIDGGMATFEVIEKIGNDLSCRC
Sbjct: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMATFEVIEKIGNDLSCRC 240

Query: 241 TDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVDFIALSFVNSADPV 300
           TDPGLFLPRAKCSFWRDGRVVVRNHESPTLS KDWSDIEFGIAEGVDFIALSFVNSADPV
Sbjct: 241 TDPGLFLPRAKCSFWRDGRVVVRNHESPTLSRKDWSDIEFGIAEGVDFIALSFVNSADPV 300

Query: 301 KHLKNYLSTKSAESVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ 360
           KHLKN+LSTK A+SVRVLAKIESLESLQNLEEIIEAS+GIMVARGDLGVEIPLEQIPAVQ
Sbjct: 301 KHLKNFLSTKPAKSVRVLAKIESLESLQNLEEIIEASEGIMVARGDLGVEIPLEQIPAVQ 360

Query: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420
           EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY
Sbjct: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420

Query: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLQDRIAEEICNSAAELANRLSVDA 480
           GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSL DRIAEEICNSAAELANRLSVDA
Sbjct: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSAAELANRLSVDA 480

Query: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540
           IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG
Sbjct: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540

Query: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 580
           IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI
Sbjct: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 579

BLAST of Cp4.1LG14g04760 vs. NCBI nr
Match: XP_022984667.1 (plastidial pyruvate kinase 1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1115 bits (2883), Expect = 0.0
Identity = 568/581 (97.76%), Postives = 575/581 (98.97%), Query Frame = 0

Query: 1   MAEVNAMMIRAGCNFVAKNPVLDVKRSGFGMPVFGRTVWCNRRRTTGRVGNGVKAVMQVD 60
           MAEVNAMMIRAGCNFVAK PVLDVKRSGFG+PVFGRTVWCNRRRT+GRVGNGVKAVMQVD
Sbjct: 1   MAEVNAMMIRAGCNFVAKKPVLDVKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVD 60

Query: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120
           LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE
Sbjct: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120

Query: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180
           GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE
Sbjct: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180

Query: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCRC 240
           DGSIWLFTAEKFEG R FTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSC+C
Sbjct: 181 DGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKC 240

Query: 241 TDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVDFIALSFVNSADPV 300
           TDPGLFLPRAK SFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGV+FIALSFVN ADPV
Sbjct: 241 TDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPV 300

Query: 301 KHLKNYLSTKSAESVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ 360
           KHLKNYLSTKSA+SVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPA+Q
Sbjct: 301 KHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQ 360

Query: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420
           EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY
Sbjct: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420

Query: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLQDRIAEEICNSAAELANRLSVDA 480
           GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSL DRIAEEICNSA+ELANRLSVDA
Sbjct: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDA 480

Query: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540
           IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG
Sbjct: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540

Query: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIA 581
           IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIA
Sbjct: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIA 581

BLAST of Cp4.1LG14g04760 vs. NCBI nr
Match: XP_038905899.1 (plastidial pyruvate kinase 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1063 bits (2748), Expect = 0.0
Identity = 543/580 (93.62%), Postives = 561/580 (96.72%), Query Frame = 0

Query: 1   MAEVNAMMIRAGCNFVAKNPVLDVKRSGFGMPVFGRTVWCNRRRTTGRVGNGVKAVMQVD 60
           MAEVNA+MIR+  +F+AK+PVLDVKRSGFGMPV GRTVWCNRRRT G VGNGVKA MQVD
Sbjct: 1   MAEVNAIMIRSRFDFIAKSPVLDVKRSGFGMPVIGRTVWCNRRRTRGGVGNGVKATMQVD 60

Query: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120
           LED ERL+RLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPAC+AIEDLEKLALE
Sbjct: 61  LEDEERLKRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACNAIEDLEKLALE 120

Query: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180
           GMNIARLNMCHNSREWHCDVIRKIKKLNE KGFCISVMIDTEGSQIHVVDHGAPSS+KVE
Sbjct: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEKKGFCISVMIDTEGSQIHVVDHGAPSSVKVE 180

Query: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCRC 240
           DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDS+VIDGGMATFEVIEKIGNDLSCRC
Sbjct: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMATFEVIEKIGNDLSCRC 240

Query: 241 TDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVDFIALSFVNSADPV 300
           TDPGLFLPRAKCSFWRDGR+VVRNHESPTLSSKDWSDI+FGI+EGVDFIALSFVNSADPV
Sbjct: 241 TDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIKFGISEGVDFIALSFVNSADPV 300

Query: 301 KHLKNYLSTKSAESVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ 360
           +HLKNYL TKSA+SVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ
Sbjct: 301 QHLKNYLLTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ 360

Query: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420
           EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY
Sbjct: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420

Query: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLQDRIAEEICNSAAELANRLSVDA 480
           GQKALSVLQMAS RME WSREENR+NFLP +QLGVSL DRIAEEICNSAAELANRLSVDA
Sbjct: 421 GQKALSVLQMASSRMESWSREENRKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVDA 480

Query: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540
           IFVLT QGHMASLLSRNRP P IFALTDDDSTRMALNLQWGV PLRI+ SEDIEANI RG
Sbjct: 481 IFVLTNQGHMASLLSRNRPSPLIFALTDDDSTRMALNLQWGVVPLRIDFSEDIEANISRG 540

Query: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 580
           IELVKSKGLVK+GDSVLVVSEISPARAASMASQSIQLKTI
Sbjct: 541 IELVKSKGLVKKGDSVLVVSEISPARAASMASQSIQLKTI 580

BLAST of Cp4.1LG14g04760 vs. ExPASy TrEMBL
Match: A0A6J1E4Y3 (Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111430639 PE=3 SV=1)

HSP 1 Score: 1119 bits (2895), Expect = 0.0
Identity = 570/580 (98.28%), Postives = 576/580 (99.31%), Query Frame = 0

Query: 1   MAEVNAMMIRAGCNFVAKNPVLDVKRSGFGMPVFGRTVWCNRRRTTGRVGNGVKAVMQVD 60
           MAEVNAMMIRAGCNFVAKNPVLD+KRSGFGMPVFGRTVWCNRR TTGRVGNGVKAVMQVD
Sbjct: 1   MAEVNAMMIRAGCNFVAKNPVLDLKRSGFGMPVFGRTVWCNRR-TTGRVGNGVKAVMQVD 60

Query: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120
           LEDAERLRRLEGLEATLGLD+VAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE
Sbjct: 61  LEDAERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120

Query: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180
           GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE
Sbjct: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180

Query: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCRC 240
           DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDS+VIDGGMATFEVIEKIGNDLSCRC
Sbjct: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMATFEVIEKIGNDLSCRC 240

Query: 241 TDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVDFIALSFVNSADPV 300
           TDPGLFLPRAKCSFWRDGRVVVRNHESPTLS KDWSDIEFGIAEGVDFIALSFVNSADPV
Sbjct: 241 TDPGLFLPRAKCSFWRDGRVVVRNHESPTLSRKDWSDIEFGIAEGVDFIALSFVNSADPV 300

Query: 301 KHLKNYLSTKSAESVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ 360
           KHLKN+LSTK A+SVRVLAKIESLESLQNLEEIIEAS+GIMVARGDLGVEIPLEQIPAVQ
Sbjct: 301 KHLKNFLSTKPAKSVRVLAKIESLESLQNLEEIIEASEGIMVARGDLGVEIPLEQIPAVQ 360

Query: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420
           EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY
Sbjct: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420

Query: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLQDRIAEEICNSAAELANRLSVDA 480
           GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSL DRIAEEICNSAAELANRLSVDA
Sbjct: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSAAELANRLSVDA 480

Query: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540
           IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG
Sbjct: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540

Query: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 580
           IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI
Sbjct: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 579

BLAST of Cp4.1LG14g04760 vs. ExPASy TrEMBL
Match: A0A6J1J2T2 (Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111482878 PE=3 SV=1)

HSP 1 Score: 1115 bits (2883), Expect = 0.0
Identity = 568/581 (97.76%), Postives = 575/581 (98.97%), Query Frame = 0

Query: 1   MAEVNAMMIRAGCNFVAKNPVLDVKRSGFGMPVFGRTVWCNRRRTTGRVGNGVKAVMQVD 60
           MAEVNAMMIRAGCNFVAK PVLDVKRSGFG+PVFGRTVWCNRRRT+GRVGNGVKAVMQVD
Sbjct: 1   MAEVNAMMIRAGCNFVAKKPVLDVKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVD 60

Query: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120
           LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE
Sbjct: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120

Query: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180
           GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE
Sbjct: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180

Query: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCRC 240
           DGSIWLFTAEKFEG R FTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSC+C
Sbjct: 181 DGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKC 240

Query: 241 TDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVDFIALSFVNSADPV 300
           TDPGLFLPRAK SFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGV+FIALSFVN ADPV
Sbjct: 241 TDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPV 300

Query: 301 KHLKNYLSTKSAESVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ 360
           KHLKNYLSTKSA+SVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPA+Q
Sbjct: 301 KHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQ 360

Query: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420
           EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY
Sbjct: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420

Query: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLQDRIAEEICNSAAELANRLSVDA 480
           GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSL DRIAEEICNSA+ELANRLSVDA
Sbjct: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDA 480

Query: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540
           IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG
Sbjct: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540

Query: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIA 581
           IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIA
Sbjct: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIA 581

BLAST of Cp4.1LG14g04760 vs. ExPASy TrEMBL
Match: A0A5A7TLC2 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00350 PE=3 SV=1)

HSP 1 Score: 1057 bits (2733), Expect = 0.0
Identity = 538/581 (92.60%), Postives = 558/581 (96.04%), Query Frame = 0

Query: 1   MAEVNAMMIRAGCNFVAKNPVLDVKRSGFGMPVFG-RTVWCNRRRTTGRVGNGVKAVMQV 60
           MAEVNAMMIRA CNF+ +NPVLDVKR GF MPV G RTVWCNRRR  GRVGNGVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  DLEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLAL 120
           DL+D ERLRRLEGLEATLGLD+VAERELKEKGFLGMRKTKLVCTIGPACS IEDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKV 180
           EGMNIARLNMCHNSREWHCD+I+KIKKLNE KGFCISVMID EGS+IHVVDHGAPSS+KV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCR 240
           EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGI+VGDS+VIDGGMATFEVIEKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGR+VVRNHESPTLSSKDWSDIEFGI+EGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VKHLKNYLSTKSAESVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           V+HLKNYL TKSA+S+RVLAKIESLE+LQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEIT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLQDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMAS RMELWSREEN +NFLP +QLGVSL DRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWR 540
           AIFVLT QGHMASLLSRNRP PPIFALTDDDSTRMALNLQWGVFPLRI+LSEDIEANI R
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 580
           GIE+VKSKGLVKQGDSVLVVSEISPARA SMASQSIQLKTI
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTI 581

BLAST of Cp4.1LG14g04760 vs. ExPASy TrEMBL
Match: A0A5D3E0J9 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00350 PE=3 SV=1)

HSP 1 Score: 1055 bits (2728), Expect = 0.0
Identity = 537/581 (92.43%), Postives = 557/581 (95.87%), Query Frame = 0

Query: 1   MAEVNAMMIRAGCNFVAKNPVLDVKRSGFGMPVFG-RTVWCNRRRTTGRVGNGVKAVMQV 60
           MAEVNAMMIRA CNF+ +NPVLDVKR GF MPV G RTVWCNRRR  GRVGNGVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  DLEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLAL 120
           DL+D ERLRRLEGLEATLGLD+VAERELKEKGFLGMRKTKLVCTIGPACS IEDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKV 180
           EGMNIARLNMCHNSREWHCD+I+KIKKLNE KGFCISVMID EGS+IHVVDHGAPSS+KV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCR 240
           EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGI+VGDS+VIDGGMATFEV EKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGR+VVRNHESPTLSSKDWSDIEFGI+EGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VKHLKNYLSTKSAESVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           V+HLKNYL TKSA+S+RVLAKIESLE+LQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEIT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLQDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMAS RMELWSREEN +NFLP +QLGVSL DRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWR 540
           AIFVLT QGHMASLLSRNRP PPIFALTDDDSTRMALNLQWGVFPLRI+LSEDIEANI R
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 580
           GIE+VKSKGLVKQGDSVLVVSEISPARA SMASQSIQLKTI
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTI 581

BLAST of Cp4.1LG14g04760 vs. ExPASy TrEMBL
Match: E5GBH7 (Pyruvate kinase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 1055 bits (2728), Expect = 0.0
Identity = 537/581 (92.43%), Postives = 557/581 (95.87%), Query Frame = 0

Query: 1   MAEVNAMMIRAGCNFVAKNPVLDVKRSGFGMPVFG-RTVWCNRRRTTGRVGNGVKAVMQV 60
           MAEVNAMMIRA CNF+ +NPVLDVKR GF MPV G RTVWCNRRR  GRVGNGVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  DLEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLAL 120
           DL+D ERLRRLEGLEATLGLD+VAERELKEKGFLGMRKTKLVCTIGPACS IEDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKV 180
           EGMNIARLNMCHNSREWHCD+I+KIKKLNE KGFCISVMID EGS+IHVVDHGAPSS+KV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCR 240
           EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGI+VGDS+VIDGGMATFEV EKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGR+VVRNHESPTLSSKDWSDIEFGI+EGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VKHLKNYLSTKSAESVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           V+HLKNYL TKSA+S+RVLAKIESLE+LQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEIT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLQDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMAS RMELWSREEN +NFLP +QLGVSL DRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWR 540
           AIFVLT QGHMASLLSRNRP PPIFALTDDDSTRMALNLQWGVFPLRI+LSEDIEANI R
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 580
           GIE+VKSKGLVKQGDSVLVVSEISPARA SMASQSIQLKTI
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTI 581

BLAST of Cp4.1LG14g04760 vs. TAIR 10
Match: AT3G22960.1 (Pyruvate kinase family protein )

HSP 1 Score: 604.4 bits (1557), Expect = 9.8e-173
Identity = 297/487 (60.99%), Postives = 372/487 (76.39%), Query Frame = 0

Query: 79  LDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHC 138
           +D V E ELKE GF   R+TKL+CTIGPA    E LE LA+ GMN+ARLNMCH +R+WH 
Sbjct: 100 VDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHR 159

Query: 139 DVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEGSRP- 198
            VIR +++LNE KGF +++M+DTEGS+IH+ D G  +S K EDG +W FT   F+ SRP 
Sbjct: 160 GVIRSVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPE 219

Query: 199 FTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCSFWRD 258
            T+  SY+GF+E ++VGD +++DGGM  FEVIEKIG D+ C CTDPGL LPRA  +FWRD
Sbjct: 220 RTISVSYDGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRD 279

Query: 259 GRVV-VRNHESPTLSSKDWSDIEFGIAEGVDFIALSFVNSADPVKHLKNYLSTKS-AESV 318
           G +V  RN   PT+SSKDW DI+FGIAEGVDFIA+SFV SA+ + HLK+YL+ +S    +
Sbjct: 280 GSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEI 339

Query: 319 RVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVI 378
            V+AKIES++SL NLEEII ASDG MVARGDLG +IPLEQ+PA Q+ I  VCR LNKPVI
Sbjct: 340 GVIAKIESIDSLTNLEEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVI 399

Query: 379 IASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRM 438
           +ASQLLESM+EYPTPTRAEVADVSEAVRQ +DALMLSGESA+G +  KAL+VL+  S R+
Sbjct: 400 VASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRI 459

Query: 439 ELWSREENRENFLPHYQLGVSLQDRIAEEICNSAAELANRLSVDAIFVLTTQGHMASLLS 498
           E W REE R   +P   +G S  D+I+EEICNSAA++AN L VDA+FV TT GHMASL+S
Sbjct: 460 ERWWREEKRHESVPLQAIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVS 519

Query: 499 RNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVKQGDS 558
           R RP  PIFA T   S R  LNLQWG+ P R+  S+D+E+N+ +   L+KS+G++K GD 
Sbjct: 520 RCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDL 579

Query: 559 VLVVSEI 563
           V+ VS++
Sbjct: 580 VIAVSDM 586

BLAST of Cp4.1LG14g04760 vs. TAIR 10
Match: AT5G52920.1 (plastidic pyruvate kinase beta subunit 1 )

HSP 1 Score: 317.0 bits (811), Expect = 3.1e-86
Identity = 184/486 (37.86%), Postives = 281/486 (57.82%), Query Frame = 0

Query: 96  RKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHCDVIRKIKKLN-EGKGFC 155
           RKTK+VCT+GP+ +  E + KLA  GMN+AR+NM H     H  VI  +K+ N + K   
Sbjct: 110 RKTKIVCTVGPSTNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNT 169

Query: 156 ISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVG 215
           I++M+DT+G ++   D   P  I ++ G  + FT E+   S P  V  +Y+ F   ++ G
Sbjct: 170 IAIMLDTKGPEVRSGD--LPQPIMLDPGQEFTFTIER-GVSTPSCVSVNYDDFVNDVEAG 229

Query: 216 DSVVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKD 275
           D +++DGGM +F V  K  + + C   D G    R      R   V  ++   P+++ KD
Sbjct: 230 DMLLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSR------RHLNVRGKSATLPSITEKD 289

Query: 276 WSDIEFGIAEGVDFIALSFVNSADPVKHLKNYLSTKSAESVRVLAKIESLESLQNLEEII 335
           W DI+FG+   VDF A+SFV  A  V  LK YL    A+ + V+ KIES +S+ NL  II
Sbjct: 290 WEDIKFGVENKVDFYAVSFVKDAQVVHELKKYLQNSGAD-IHVIVKIESADSIPNLHSII 349

Query: 336 EASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIIASQLLESMVEYPTPTRAE 395
            ASDG MVARGDLG E+P+E++P +QEEI ++CR + K VI+A+ +LESM+ +PTPTRAE
Sbjct: 350 TASDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPTRAE 409

Query: 396 VADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRMELWSREENRENFLPHYQLG 455
           V+D++ AVR+ ADA+MLSGE+A G +  KA  V+   + R E           +P   LG
Sbjct: 410 VSDIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVALRTEATITSGE----MPP-NLG 469

Query: 456 VSLQDRIAEEICNSAAELANRLSVDAIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRM 515
            + ++ ++E     A  ++N L    + V T  G MA LLS  RP   I+A T++   + 
Sbjct: 470 QAFKNHMSEMFAYHATMMSNTLGTSTV-VFTRTGFMAILLSHYRPSGTIYAFTNEKKIQQ 529

Query: 516 ALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVKQGDSVLVVSEISPARAASMASQS 575
            L L  GV P+ +E ++D E      +  +  +G+VK+G+ + +V   +     S ++ +
Sbjct: 530 RLALYQGVCPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPIWRSQSTHN 579

Query: 576 IQLKTI 581
           IQ++ +
Sbjct: 590 IQVRKV 579

BLAST of Cp4.1LG14g04760 vs. TAIR 10
Match: AT1G32440.1 (plastidial pyruvate kinase 3 )

HSP 1 Score: 310.1 bits (793), Expect = 3.8e-84
Identity = 190/486 (39.09%), Postives = 280/486 (57.61%), Query Frame = 0

Query: 96  RKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHCDVIRKIKKLNE-GKGFC 155
           RKTK+VCTIGP+ S+ E + KLA  GMN+ARLNM H     H   I  +K+ N       
Sbjct: 99  RKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMSHGDHASHQITIDLVKEYNSLFVDKA 158

Query: 156 ISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVG 215
           I++M+DT+G ++   D   P  I +E+G  + FT ++    +  TV  +Y+ F   ++VG
Sbjct: 159 IAIMLDTKGPEVRSGD--VPQPIFLEEGQEFNFTIKRGVSLKD-TVSVNYDDFVNDVEVG 218

Query: 216 DSVVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKD 275
           D +++DGGM +  V  K  + + C   D G    R      R   V  ++   P+++ KD
Sbjct: 219 DILLVDGGMMSLAVKSKTSDLVKCVVIDGGELQSR------RHLNVRGKSATLPSITDKD 278

Query: 276 WSDIEFGIAEGVDFIALSFVNSADPVKHLKNYLSTKSAESVRVLAKIESLESLQNLEEII 335
           W DI+FG+   VDF A+SFV  A  V  LKNYL T SA+ + V+ KIES +S++NL  II
Sbjct: 279 WEDIKFGVDNQVDFYAVSFVKDAKVVHELKNYLKTCSAD-ISVIVKIESADSIKNLPSII 338

Query: 336 EASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIIASQLLESMVEYPTPTRAE 395
            A DG MVARGDLG E+P+E++P +QEEI   CR ++KPVI+A+ +LESM+ +PTPTRAE
Sbjct: 339 SACDGAMVARGDLGAELPIEEVPLLQEEIIRRCRSIHKPVIVATNMLESMINHPTPTRAE 398

Query: 396 VADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRMELWSREENRENFLPHYQLG 455
           V+D++ AVR+ ADA+MLSGE+A G +  KA++V+   + R E         +    Y   
Sbjct: 399 VSDIAIAVREGADAIMLSGETAHGKFPLKAVNVMHTVALRTEASLPVRTSASRTTAY--- 458

Query: 456 VSLQDRIAEEICNSAAELANRLSVDAIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRM 515
              +  + +     A+ +AN LS   + V T  G MA LLS  RP   IFA T+      
Sbjct: 459 ---KGHMGQMFAFHASIMANTLS-SPLIVFTRTGSMAVLLSHYRPSATIFAFTNQRRIMQ 518

Query: 516 ALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVKQGDSVLVVSEISPARAASMASQS 575
            L L  GV P+ +E S+D E    R ++L++ + ++K+G  V +V   S       ++  
Sbjct: 519 RLALYQGVMPIYMEFSDDAEDTYARSLKLLQDENMLKEGQHVTLVQSGSQPIWREESTHL 567

Query: 576 IQLKTI 581
           IQ++ I
Sbjct: 579 IQVRKI 567

BLAST of Cp4.1LG14g04760 vs. TAIR 10
Match: AT3G04050.1 (Pyruvate kinase family protein )

HSP 1 Score: 231.5 bits (589), Expect = 1.7e-60
Identity = 162/509 (31.83%), Postives = 259/509 (50.88%), Query Frame = 0

Query: 97  KTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCIS 156
           KTK+VCT+GPA  ++E +EKL   GMN+AR N  H S  +H + +  ++   E      +
Sbjct: 17  KTKIVCTLGPASRSVEMVEKLLKAGMNVARFNFSHGSHSYHQETLDNLRTAMENTCIPCA 76

Query: 157 VMIDTEGSQIHV--VDHGAPSSIKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVG 216
           VM+DT+G +I    +  G P  + ++   I + T    EG    T+  SY+  +E ++ G
Sbjct: 77  VMLDTKGPEIRTGFLKEGKPVEL-IQGQEITISTDYTMEGDSN-TISMSYKKLAEDLKSG 136

Query: 217 DSVVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCSFWRDGRVVVRNHES------- 276
           D ++   G  +  V       LSC   D  L L RA+C    +   V+   ++       
Sbjct: 137 DVILCSDGTISLTV-------LSC---DKNLGLVRARC----ENSAVLGERKNVNLPGIV 196

Query: 277 ---PTLSSKDWSDI-EFGIAEGVDFIALSFVNSADPVKHLKNYLSTKSAESVRVLAKIES 336
              PTL+ KD  DI ++G+   +D IALSFV     +  ++  L  ++A+S+ +++K+E+
Sbjct: 197 VDLPTLTEKDQEDILQWGVPNKIDIIALSFVRKGSDLVEVRKLLG-ENAKSIMLMSKVEN 256

Query: 337 LESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIIASQLLES 396
            E + N ++I+E SD  MVARGDLG+EIP+E++   Q+ +      L KP++ A+Q+LES
Sbjct: 257 QEGVMNFDKILEYSDAFMVARGDLGMEIPIEKMFLAQKMMIQKANALGKPIVTATQMLES 316

Query: 397 MVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRMELWSREEN 456
           M + P PTRAE  DV+ AV    D +MLSGE+A G++ + A  VL M+    E       
Sbjct: 317 MTKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAHPETA--VLTMSRICKE------- 376

Query: 457 RENFLPHYQLGVSLQDRIA------EEICNSAAELANRLSVDAIFVLTTQGHMASLLSRN 516
            E+F+ +  +   +QD ++      E +  SA   A  L   AI VLT  G+   L+++ 
Sbjct: 377 AEDFIDYDTMHKKIQDIVSLPLSPIESLAASAVSTARSLCAAAIVVLTKGGYTVELVAKY 436

Query: 517 RPRPPIFALTDDDSTRMALNLQW-----------------GVFPL-------RIELSEDI 563
           RP  PI ++   + TR   + +W                 G+ P+       R    +  
Sbjct: 437 RPSVPILSVIVPEITRTD-DFEWSCSESAAHVARRGLIYRGIVPVMATGASARSSNKDST 496

BLAST of Cp4.1LG14g04760 vs. TAIR 10
Match: AT5G63680.1 (Pyruvate kinase family protein )

HSP 1 Score: 229.6 bits (584), Expect = 6.6e-60
Identity = 158/494 (31.98%), Postives = 254/494 (51.42%), Query Frame = 0

Query: 97  KTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCIS 156
           KTK+VCT+GPA  ++  +EKL   GMN+AR N  H S E+H + +  ++   +  G   +
Sbjct: 21  KTKIVCTLGPASRSVTMIEKLLKAGMNVARFNFSHGSHEYHQETLDNLRTAMQNTGILAA 80

Query: 157 VMIDTEGSQIHV--VDHGAPSSIKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVG 216
           VM+DT+G +I    +  G P  +K E   I + T    +G    T+  SY+     ++ G
Sbjct: 81  VMLDTKGPEIRTGFLKDGNPIQLK-EGQEITITTDYDIKGDEK-TISMSYKKLPVDVKPG 140

Query: 217 DSVVIDGGMATFEVI--EKIGNDLSCRCTDPGLFLPRAKCSFWRDGRVVVRNHESPTLSS 276
           ++++   G  +  V+  +     + CRC +  +   R   +    G VV    + PTL+ 
Sbjct: 141 NTILCADGSISLAVVSCDPNAGTVICRCENTAMLGERKNVNL--PGVVV----DLPTLTD 200

Query: 277 KDWSDI-EFGIAEGVDFIALSFVNSADPVKHLKNYLSTKSAESVRVLAKIESLESLQNLE 336
           KD  DI ++G+   +D IALSFV     + +++  L + S +S+ +++K+E+ E + N +
Sbjct: 201 KDVEDILKWGVPNNIDMIALSFVRKGSDLVNVRKVLGSHS-KSIMLMSKVENQEGVLNFD 260

Query: 337 EIIEASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIIASQLLESMVEYPTPT 396
           EI+  +D  MVARGDLG+EIP+E+I   Q+ +   C    KPV+ A+Q+LESM++ P PT
Sbjct: 261 EILRETDAFMVARGDLGMEIPIEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPT 320

Query: 397 RAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRMELWSREENRENFLPHY 456
           RAE  DV+ AV    D +MLSGESA G+Y +  ++V  MA   +E  S  +    F    
Sbjct: 321 RAEATDVANAVLDGTDCVMLSGESAAGAYPE--IAVKTMAKICIEAESSLDYNTIFKEMI 380

Query: 457 QLGVSLQDRIAEEICNSAAELANRLSVDAIFVLTTQGHMASLLSRNRPRPPIFAL----- 516
           +    L     E + +SA   AN+     I VLT  G  A L+++ RP  PI ++     
Sbjct: 381 R-ATPLPMSTLESLASSAVRTANKAKAKLIIVLTRGGTTAKLVAKYRPAVPILSVVVPVF 440

Query: 517 ---------TDDDSTRMALNLQWGVFPL------RIELSEDIEANIWRGIELVKSKGLVK 566
                    +D+   R +L +  G+ P+      +   SE  E  I   ++    KGL  
Sbjct: 441 TSDTFNWSCSDESPARHSL-IYRGLIPVLGEGSAKATDSESTEEIIESALKSATEKGLCN 500

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LIK01.4e-17160.99Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q431171.5e-17059.47Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1[more]
Q405456.7e-16658.25Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q405464.0e-8639.78Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9FLW94.4e-8537.86Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_023553283.10.0100.00plastidial pyruvate kinase 1, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
KAG7014927.10.098.79Plastidial pyruvate kinase 1, chloroplastic [Cucurbita argyrosperma subsp. argyr... [more]
XP_022922736.10.098.28plastidial pyruvate kinase 1, chloroplastic-like [Cucurbita moschata][more]
XP_022984667.10.097.76plastidial pyruvate kinase 1, chloroplastic-like [Cucurbita maxima][more]
XP_038905899.10.093.62plastidial pyruvate kinase 1, chloroplastic-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1E4Y30.098.28Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111430639 PE=3 SV=1[more]
A0A6J1J2T20.097.76Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111482878 PE=3 SV=1[more]
A0A5A7TLC20.092.60Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00350... [more]
A0A5D3E0J90.092.43Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G0035... [more]
E5GBH70.092.43Pyruvate kinase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G22960.19.8e-17360.99Pyruvate kinase family protein [more]
AT5G52920.13.1e-8637.86plastidic pyruvate kinase beta subunit 1 [more]
AT1G32440.13.8e-8439.09plastidial pyruvate kinase 3 [more]
AT3G04050.11.7e-6031.83Pyruvate kinase family protein [more]
AT5G63680.16.6e-6031.98Pyruvate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001697Pyruvate kinasePRINTSPR01050PYRUVTKNASEcoord: 317..343
score: 52.48
coord: 369..393
score: 57.66
coord: 344..368
score: 48.92
coord: 413..429
score: 35.11
coord: 286..300
score: 47.28
coord: 153..169
score: 27.42
coord: 394..412
score: 54.25
IPR001697Pyruvate kinaseTIGRFAMTIGR01064TIGR01064coord: 96..567
e-value: 4.2E-122
score: 406.0
IPR001697Pyruvate kinasePANTHERPTHR11817PYRUVATE KINASEcoord: 39..580
IPR036918Pyruvate kinase, C-terminal domain superfamilyGENE3D3.40.1380.20coord: 436..562
e-value: 5.8E-139
score: 465.6
IPR036918Pyruvate kinase, C-terminal domain superfamilySUPERFAMILY52935PK C-terminal domain-likecoord: 459..562
IPR015806Pyruvate kinase, insert domain superfamilyGENE3D2.40.33.10coord: 164..268
e-value: 5.8E-139
score: 465.6
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 96..435
e-value: 5.8E-139
score: 465.6
IPR015795Pyruvate kinase, C-terminalPFAMPF02887PK_Ccoord: 463..563
e-value: 9.1E-21
score: 74.3
IPR015793Pyruvate kinase, barrelPFAMPF00224PKcoord: 95..433
e-value: 2.5E-86
score: 289.7
NoneNo IPR availablePANTHERPTHR11817:SF21PYRUVATE KINASEcoord: 39..580
IPR018209Pyruvate kinase, active sitePROSITEPS00110PYRUVATE_KINASEcoord: 315..327
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 90..436
IPR011037Pyruvate kinase-like, insert domain superfamilySUPERFAMILY50800PK beta-barrel domain-likecoord: 166..253

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG14g04760.1Cp4.1LG14g04760.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0016301 kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030955 potassium ion binding
molecular_function GO:0004743 pyruvate kinase activity
molecular_function GO:0003824 catalytic activity