Cp4.1LG14g04520 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG14g04520
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Adenylyl-sulfate kinase
Location	Cp4.1LG14: 1526369 .. 1529842 (-)
RNA-Seq Expression	Cp4.1LG14g04520
Synteny	Cp4.1LG14g04520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGTCGTCGTGTCGGGTCCTTCCAACGACAACTTATTGCGTTATTACACCGTCGGGTCTTAAACGGCGTCGTTCGCTTCTTCTTCGCCGGACGGCTCGCTGTTCCGCACGCTCCGGCTCCGCATTCTTCTTCTACAGTTCATAAATTTCCGGGAAGAAGGATAATCAAAATCAAAGGTTCAAAACGAGGGGTCGTTGCCAAAAGGGCTCGTGATTCTATTTTTTTTCTTCACCGGTCATTAATTGTTATGGCGGAAAAGAACAGGATAGAGCCTCCCCTATTCGACCAATAATGGCTTCCCATTTCCCCTCGATTTCCTCACTTGGTTCTTATTTTTTCGTTTCTCTATTTATTATTGATTCGATAGTGTTTTCCTATTGAGATGTGCGTGAGATATGGTTGCGATTGGGGGAATTTCGTTGCCGCGTTTCATAGCGCCGTCGCTTCACCGCCAGTCCTTTTCGCCGCCGGCGATTGGGTTTGCGAGGTTTCCCAATAGGATTAGGCTTAGATCGACAGTGGCTTTGCTGGATGAGGTTGAGAAGACGGGTTCGAGGGCGGCGATTGTCAATCGGAAGGTCGATGGTTTGGTTAAGAGTGATTGTGAGACTGATTTCGATGCTACCTTGGGCAATGGACATGCTAGGAACTCTGGTATTGCTTCTATTTTGATTTATTTTGATATTAGTGGTGTCGAATGTTGTTCTTCATGGTCTGAGTTTAAGCTGTCCAGATTAACAAAATGCTTATCAATCAGGCAAAAATCTTGGCGTGTTGTCCACCGTTGGAAATTCTACTAACATCAAATGGCATGAATGTTCTGTTGGAAAGATTGAAAGACAGAGCCTACTAGAACAGAAAGGATGTGTCATATGGATCACTGGTCTTAGTGGCTCAGGTTTGCCAATTTAGCCTTAGCTATGATCAATTAACCTGTTGTTTAGATAATCTTCAAATGAAATTGGTCGAGTTCGTGTATGCATCGGTTAACCGACACGATTCTTTTGTTGTAAACTTGGATAATTGATCAAACCTAACCATGAGATGTTTGAGTTTGATTTTTCTGGTCATCTTTTAAAAGATTTATGTGAAAAGGATAGCAAAAATGTTGGCTAAGTTGGGTTTGGAACTCTTTGGAGCTGACCCAATTTGTCTAAATATGCTTAGCTAACTTGCAGGGAAGAGCTGTGTGGCCTGTGCCTTGAATCAAATCTTATACAAAATGGGGAAGTTGGCTTATATCCTTGATGGGGATAATGTCAGGCATGGCTTGAACCGCGACCTTGGTTTTAAAGCGGAAGATCGTGCCGAGAACATAAGGAGGATCGGTGAGTCTTGCTTGGGAAGTTCGGTTTATTTATAACGGTTTGGATTGGGCTGTTCATGATTGCTAAGTATATCTCTTCTGTAAATTAGGTGAGGTTGCGAAACTGTTTGCCAATGCCGGAGTTATTTGCATTGCTAGTTTGATATCTCCATATCGAAGGGATCGAGATGCTTGTCGTGCCATTTTGCCTGATGGATATTTTATTGAGGTTGTTCTTCATTTATGCATACCATGGATTTTGAGCAGGCTTCTATTTTACCTTGCATGACAAAAATGTGCCAAAAATATATAGGTGTTCATGGATGTTTCTCTTGAAGTTTGTGAAGCAAGAGATGCAAAAGGACTGTATAAGCTTGCACGGGCGGGGAAGATCAAAGGTCACTGAAACTTGTTATCATCTTCCTTAGATTTAACTGATGATCTTAAATATTGGCTCGAAGTTCATAACTTGTCGATCGACTGAACGAAATCTCATATTTTGCAGGCTTTACCGGTATTGATGATCCGTATGAAGTACCATTGAACTGTGAGGTAATTCCAAATTCTAGAGGAAAATGGATCATATCTTTTAAACATTCTTGCAAAAGATTAATGCTTCTACTGCATTGAGGAGATTCCATATCGGTTGGAGAGGGGAACGAAACATTCCTTATAAGGGTGTGGAAACCACTCCCTAGTATACGTGTTTTAAAATTGTAAGAGGTGAACAATATCTACTTGCGTTGAGCTTGGGTTGTTACAAATGATATCATAGCTAAACACTAGGGGGTGTGCCACCGAGGACGCTGGGCTCCAAGGGGGGTGGATTGTGAGACCTACATCAGTTGGAGAGGGGAATGATACATTTCTTACAAGGATGTGGAAACCTCTCCCTAGTAGATGTGTTTTAAAACCGTAAGGCTAATAGTGATATGTAACGCGTCAAAGCGGACAATATCTACTAACGGTGGGCTTGGGCTGTTACAAATGATATTAGAGTCAGACACCGGACAGTGTGTCAGCGAGAATGCTGGACTCCAAGGGGGTAGATTGTGAGATCCCACATCGGTTGGAGAGGGGAACGAAACATTCCTTATATGGGTGTGGAAACCACTCCCTAGTAGACGTGTTTTAAAATTGTAAGAAGTGAACAATATCTACTTGCGGTGGGCTTGGGTTGTTACCAATGATATCATTGCTAAACACTGGGGGGGTGTGCCACTGAGGATGCTGGGCTCCAAGGGGGAGTGGATTGTGAGATACTACATCGATTGGAGAGGGGAACGATACATTTCTTACAAGAGTGTGGAAACCTCTCCCTAGTAGACGCGTTTTAAAACCGTAAGGCTAATAGTGATACGTAACGGGTCAAAGCAGATAGTACGGTGGGCTTGGACTGTTACAATTGATATTAGAGCCAGACACTGGGCGGTGTGTCAGCGTCCTTGCTGGGCCCCAAGAGAGGTGGATTATGAGATCTCACATCAATTGGAGAGGGGAACGAAACATTCCTTATAAGGGTGTGGAAACCACTCCCTAGTAGATGCATTTTAAAATTGTAAGAAGTGAACAATATCTACTTGCAGTGGGCTTAGATTGTTACAAATGATATCATAGCTAAACATTGGGGGTGTGCCAGCGAGGACGCTGGGCTCCAAGGGGGTGGATTGCGAGATCCTACATCGGTTGGAGAGGGGAACGATACATTTCTTACAAGGGTGTGTAAACCTCTCCCTAGTAGACGCGTTTTAAAACTGTAAAGCTAATAGTGACGCGTAACGGGCCAAAACGGATAATATCTACTCATGGTGGGCTTAGAAGAAGACTAATGAATATAACGTCTTGTGTTATAAATGATTCATTGTTTACGAAGCTGATTGGGAGCGGTTTCAGATAGTACTGAAGAACAATGGAGGCTCCCCATGTGAGATGGCAGAGAAAGTAATGAGTTACCTTGAGCAGAAGGGCTTCCTGCAGGCTTAAAACTCAAACCCTACTAGAAAGGGGGCTTTAACCCTACTTGACCGACCACAGCTTTGTTTGGTTGCGTCTGATTTGGTTCTTCTTTATCCAATTCTTTTCTGCAATACAGATAGGAACGAAGGCAATTCACCTTGCCATATATGTAATGTAGACTTCTTTCAAACAGGGTATGTATATATATGGTAT

mRNA sequence

CGTCGTCGTGTCGGGTCCTTCCAACGACAACTTATTGCGTTATTACACCGTCGGGTCTTAAACGGCGTCGTTCGCTTCTTCTTCGCCGGACGGCTCGCTGTTCCGCACGCTCCGGCTCCGCATTCTTCTTCTACAGTTCATAAATTTCCGGGAAGAAGGATAATCAAAATCAAAGGTTCAAAACGAGGGGTCGTTGCCAAAAGGGCTCGTGATTCTATTTTTTTTCTTCACCGGTCATTAATTGTTATGGCGGAAAAGAACAGGATAGAGCCTCCCCTATTCGACCAATAATGGCTTCCCATTTCCCCTCGATTTCCTCACTTGGTTCTTATTTTTTCGTTTCTCTATTTATTATTGATTCGATAGTGTTTTCCTATTGAGATGTGCGTGAGATATGGTTGCGATTGGGGGAATTTCGTTGCCGCGTTTCATAGCGCCGTCGCTTCACCGCCAGTCCTTTTCGCCGCCGGCGATTGGGTTTGCGAGGTTTCCCAATAGGATTAGGCTTAGATCGACAGTGGCTTTGCTGGATGAGGTTGAGAAGACGGGTTCGAGGGCGGCGATTGTCAATCGGAAGGTCGATGGTTTGGTTAAGAGTGATTGTGAGACTGATTTCGATGCTACCTTGGGCAATGGACATGCTAGGAACTCTGGCAAAAATCTTGGCGTGTTGTCCACCGTTGGAAATTCTACTAACATCAAATGGCATGAATGTTCTGTTGGAAAGATTGAAAGACAGAGCCTACTAGAACAGAAAGGATGTGTCATATGGATCACTGGTCTTAGTGGCTCAGGGAAGAGCTGTGTGGCCTGTGCCTTGAATCAAATCTTATACAAAATGGGGAAGTTGGCTTATATCCTTGATGGGGATAATGTCAGGCATGGCTTGAACCGCGACCTTGGTTTTAAAGCGGAAGATCGTGCCGAGAACATAAGGAGGATCGGTGAGGTTGCGAAACTGTTTGCCAATGCCGGAGTTATTTGCATTGCTAGTTTGATATCTCCATATCGAAGGGATCGAGATGCTTGTCGTGCCATTTTGCCTGATGGATATTTTATTGAGGTGTTCATGGATGTTTCTCTTGAAGTTTGTGAAGCAAGAGATGCAAAAGGACTGTATAAGCTTGCACGGGCGGGGAAGATCAAAGGCTTTACCGGTATTGATGATCCGTATGAAGTACCATTGAACTGTGAGATAGTACTGAAGAACAATGGAGGCTCCCCATGTGAGATGGCAGAGAAAGTAATGAGTTACCTTGAGCAGAAGGGCTTCCTGCAGGCTTAAAACTCAAACCCTACTAGAAAGGGGGCTTTAACCCTACTTGACCGACCACAGCTTTGTTTGGTTGCGTCTGATTTGGTTCTTCTTTATCCAATTCTTTTCTGCAATACAGATAGGAACGAAGGCAATTCACCTTGCCATATATGTAATGTAGACTTCTTTCAAACAGGGTATGTATATATATGGTAT

Coding sequence (CDS)

ATGGTTGCGATTGGGGGAATTTCGTTGCCGCGTTTCATAGCGCCGTCGCTTCACCGCCAGTCCTTTTCGCCGCCGGCGATTGGGTTTGCGAGGTTTCCCAATAGGATTAGGCTTAGATCGACAGTGGCTTTGCTGGATGAGGTTGAGAAGACGGGTTCGAGGGCGGCGATTGTCAATCGGAAGGTCGATGGTTTGGTTAAGAGTGATTGTGAGACTGATTTCGATGCTACCTTGGGCAATGGACATGCTAGGAACTCTGGCAAAAATCTTGGCGTGTTGTCCACCGTTGGAAATTCTACTAACATCAAATGGCATGAATGTTCTGTTGGAAAGATTGAAAGACAGAGCCTACTAGAACAGAAAGGATGTGTCATATGGATCACTGGTCTTAGTGGCTCAGGGAAGAGCTGTGTGGCCTGTGCCTTGAATCAAATCTTATACAAAATGGGGAAGTTGGCTTATATCCTTGATGGGGATAATGTCAGGCATGGCTTGAACCGCGACCTTGGTTTTAAAGCGGAAGATCGTGCCGAGAACATAAGGAGGATCGGTGAGGTTGCGAAACTGTTTGCCAATGCCGGAGTTATTTGCATTGCTAGTTTGATATCTCCATATCGAAGGGATCGAGATGCTTGTCGTGCCATTTTGCCTGATGGATATTTTATTGAGGTGTTCATGGATGTTTCTCTTGAAGTTTGTGAAGCAAGAGATGCAAAAGGACTGTATAAGCTTGCACGGGCGGGGAAGATCAAAGGCTTTACCGGTATTGATGATCCGTATGAAGTACCATTGAACTGTGAGATAGTACTGAAGAACAATGGAGGCTCCCCATGTGAGATGGCAGAGAAAGTAATGAGTTACCTTGAGCAGAAGGGCTTCCTGCAGGCTTAA

Protein sequence

MVAIGGISLPRFIAPSLHRQSFSPPAIGFARFPNRIRLRSTVALLDEVEKTGSRAAIVNRKVDGLVKSDCETDFDATLGNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQKGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRRIGEVAKLFANAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVMSYLEQKGFLQA

Homology

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BLAST of Cp4.1LG14g04520 vs. ExPASy Swiss-Prot
Match: Q43295 (Adenylyl-sulfate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK1 PE=1 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 6.7e-98
Identity = 173/218 (79.36%), Postives = 192/218 (88.07%), Query Frame = 0


Query: 80  NGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQKGCVIWITGLSGSGKSCVA 139
           NGH   +G+  G LSTVGNSTNIKWHECSV K++RQ LL+QKGCVIW+TGLSGSGKS +A
Sbjct: 62  NGH---TGQKQGPLSTVGNSTNIKWHECSVEKVDRQRLLDQKGCVIWVTGLSGSGKSTLA 121

Query: 140 CALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRRIGEVAKLFANAGVICIA 199
           CALNQ+LY+ GKL YILDGDNVRHGLNRDL FKAEDRAENIRR+GEVAKLFA+AG+ICIA
Sbjct: 122 CALNQMLYQKGKLCYILDGDNVRHGLNRDLSFKAEDRAENIRRVGEVAKLFADAGIICIA 181

Query: 200 SLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKGLYKLARAGKIKGFTGIDDP 259
           SLISPYR DRDACR++LP+G F+EVFMDV L VCEARD KGLYKLARAGKIKGFTGIDDP
Sbjct: 182 SLISPYRTDRDACRSLLPEGDFVEVFMDVPLSVCEARDPKGLYKLARAGKIKGFTGIDDP 241

Query: 260 YEVPLNCEIVLKNNGG-SPCEMAEKVMSYLEQKGFLQA 297
           YE PLNCEI L   GG SP EMAEKV+ YL+ KG+LQA
Sbjct: 242 YEPPLNCEISLGREGGTSPIEMAEKVVGYLDNKGYLQA 276

[Close]

BLAST of Cp4.1LG14g04520 vs. ExPASy Swiss-Prot
Match: O49204 (Adenylyl-sulfate kinase, chloroplastic OS=Catharanthus roseus OX=4058 GN=AKN PE=2 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 2.6e-89
Identity = 169/245 (68.98%), Postives = 197/245 (80.41%), Query Frame = 0


Query: 58  VNRKVDG---LVKSDCETDFDATLGNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIER 117
           VN KVD    L  SDC ++ D++L N +    GK +   +TVGNSTNI WH+C+V K ER
Sbjct: 69  VNGKVDNFRHLQPSDCNSNSDSSLNNCNG-FPGKKILQTTTVGNSTNILWHKCAVEKSER 128

Query: 118 QSLLEQKGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAE 177
           Q  L+Q+GCVIWITGLSGSGKS +ACAL++ L+  GKL YILDGDNVRHGLN DL FKAE
Sbjct: 129 QEPLQQRGCVIWITGLSGSGKSTLACALSRGLHAKGKLTYILDGDNVRHGLNSDLSFKAE 188

Query: 178 DRAENIRRIGEVAKLFANAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCE 237
           DRAENIRRIGEVAKLFA+AGVICIASLISPYR+  DACR++LP+G FIEVFMDV L+VCE
Sbjct: 189 DRAENIRRIGEVAKLFADAGVICIASLISPYRKPPDACRSLLPEGDFIEVFMDVPLKVCE 248

Query: 238 ARDAKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGG---SPCEMAEKVMSYLEQK 297
           ARD KGLYKLARAGKIKGFTGIDDPYE PL  EIVL    G   SPC++A+ V+SYLE+ 
Sbjct: 249 ARDPKGLYKLARAGKIKGFTGIDDPYEPPLKSEIVLHQKLGMCDSPCDLADIVISYLEEN 308

[Close]

BLAST of Cp4.1LG14g04520 vs. ExPASy Swiss-Prot
Match: Q9SRW7 (Adenylyl-sulfate kinase 3 OS=Arabidopsis thaliana OX=3702 GN=APK3 PE=1 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 2.0e-86
Identity = 154/206 (74.76%), Postives = 177/206 (85.92%), Query Frame = 0


Query: 93  LSTVGNSTNIKWHECSVGKIERQSLLEQKGCVIWITGLSGSGKSCVACALNQILYKMGKL 152
           +STVGNSTNI W E  +GK ERQ LL QKGCV+WITGLSGSGKS +AC+L++ L   GKL
Sbjct: 1   MSTVGNSTNIFWQESPIGKTERQKLLNQKGCVVWITGLSGSGKSTLACSLSRELNNRGKL 60

Query: 153 AYILDGDNVRHGLNRDLGFKAEDRAENIRRIGEVAKLFANAGVICIASLISPYRRDRDAC 212
           +YILDGDN+RHGLN+DLGFKAEDR ENIRR+GEVAKLFA+AG+ICIASLISPYR+DRDAC
Sbjct: 61  SYILDGDNLRHGLNKDLGFKAEDRVENIRRVGEVAKLFADAGLICIASLISPYRKDRDAC 120

Query: 213 RAILPDGYFIEVFMDVSLEVCEARDAKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKN 272
           R ++ +  FIEVFM++SL++CEARD KGLYKLARAGKIKGFTGIDDPYE PLNCEI LK 
Sbjct: 121 REMIQNSSFIEVFMNMSLQLCEARDPKGLYKLARAGKIKGFTGIDDPYESPLNCEIELKE 180

Query: 273 NGG---SPCEMAEKVMSYLEQKGFLQ 296
             G   SP  MAE+V+SYLE KGFLQ
Sbjct: 181 KEGECPSPVAMAEEVISYLEDKGFLQ 206

[Close]

BLAST of Cp4.1LG14g04520 vs. ExPASy Swiss-Prot
Match: O49196 (Adenylyl-sulfate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK2 PE=1 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 6.6e-85
Identity = 164/276 (59.42%), Postives = 197/276 (71.38%), Query Frame = 0


Query: 24  PPAIGFARFPNRIRLRSTVALLDEVEKTGSRAAIVNRKVDGLVKSDCETDFDATLGNGHA 83
           PP+ GF + P       +  L+         +A VN      + +D     +  L  G  
Sbjct: 28  PPSDGFLKLPASSIPADSRKLVANSTSFHPISA-VNVSAQASLTADFPALSETILKEG-- 87

Query: 84  RNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQKGCVIWITGLSGSGKSCVACALN 143
           RN+GK          + NI WHE S+ + +RQ LL+QKGCV+WITGLSGSGKS VACAL+
Sbjct: 88  RNNGKE--------KAENIVWHESSICRCDRQQLLQQKGCVVWITGLSGSGKSTVACALS 147

Query: 144 QILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRRIGEVAKLFANAGVICIASLIS 203
           + L++ GKL Y LDGDNVRHGLNRDL FKAE R ENIRRIGEVAKLFA+ GVICIASLIS
Sbjct: 148 KALFERGKLTYTLDGDNVRHGLNRDLTFKAEHRTENIRRIGEVAKLFADVGVICIASLIS 207

Query: 204 PYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKGLYKLARAGKIKGFTGIDDPYEVP 263
           PYRRDRDACR++LPDG F+EVFMDV L VCE+RD KGLYKLARAGKIKGFTGIDDPYE P
Sbjct: 208 PYRRDRDACRSLLPDGDFVEVFMDVPLHVCESRDPKGLYKLARAGKIKGFTGIDDPYEAP 267

Query: 264 LNCEIVLKNNGG----SPCEMAEKVMSYLEQKGFLQ 296
           +NCE+VLK+ G     SP +MAE ++SYL+ KG+L+
Sbjct: 268 VNCEVVLKHTGDDESCSPRQMAENIISYLQNKGYLE 292

[Close]

BLAST of Cp4.1LG14g04520 vs. ExPASy Swiss-Prot
Match: Q84JF0 (Adenylyl-sulfate kinase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK4 PE=1 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 3.7e-80
Identity = 144/217 (66.36%), Postives = 171/217 (78.80%), Query Frame = 0


Query: 97  GNSTNIKWHECSVGKIERQSLLEQKGCVIWITGLSGSGKSCVACALNQILYKMGKLAYIL 156
           G   NI WH+C V K +RQ L++QKGCVIWITGLSGSGKS +ACAL++ L+  GKL+YIL
Sbjct: 84  GKQKNIVWHDCPVTKSDRQELIKQKGCVIWITGLSGSGKSSLACALSRALHNRGKLSYIL 143

Query: 157 DGDNVRHGLNRDLGFKAEDRAENIRRIGEVAKLFANAGVICIASLISPYRRDRDACRAIL 216
           DGDNVRHGLN DL F+A+DRAENIRR+GEVAKLFA++G+ICIASLISPYR +R ACRA+L
Sbjct: 144 DGDNVRHGLNSDLSFEADDRAENIRRVGEVAKLFADSGIICIASLISPYRIERAACRALL 203

Query: 217 PDGYFIEVFMDVSLEVCEARDAKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKN---- 276
           P G FIEVFMDV L VCEARD KGLYK ARAGKIKGFTG+DDPYE PL+CEIV++N    
Sbjct: 204 PQGDFIEVFMDVPLHVCEARDPKGLYKRARAGKIKGFTGVDDPYEAPLDCEIVIQNSRDK 263

Query: 277 --------------NGGSPCEMAEKVMSYLEQKGFLQ 296
                         +  S CEMA+ V+SYL+Q G+L+
Sbjct: 264 GLSSSSSSSSSPSSSSSSLCEMADIVVSYLDQNGYLK 300

[Close]

BLAST of Cp4.1LG14g04520 vs. NCBI nr
Match: XP_023551902.1 (adenylyl-sulfate kinase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 594 bits (1531), Expect = 4.36e-214
Identity = 296/296 (100.00%), Postives = 296/296 (100.00%), Query Frame = 0


Query: 1   MVAIGGISLPRFIAPSLHRQSFSPPAIGFARFPNRIRLRSTVALLDEVEKTGSRAAIVNR 60
           MVAIGGISLPRFIAPSLHRQSFSPPAIGFARFPNRIRLRSTVALLDEVEKTGSRAAIVNR
Sbjct: 1   MVAIGGISLPRFIAPSLHRQSFSPPAIGFARFPNRIRLRSTVALLDEVEKTGSRAAIVNR 60

Query: 61  KVDGLVKSDCETDFDATLGNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ 120
           KVDGLVKSDCETDFDATLGNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ
Sbjct: 61  KVDGLVKSDCETDFDATLGNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ 120

Query: 121 KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRIGEVAKLFANAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKG 240
           RRIGEVAKLFANAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKG
Sbjct: 181 RRIGEVAKLFANAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVMSYLEQKGFLQA 296
           LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVMSYLEQKGFLQA
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVMSYLEQKGFLQA 296

[Close]

BLAST of Cp4.1LG14g04520 vs. NCBI nr
Match: KAG6576911.1 (Adenylyl-sulfate kinase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014937.1 Adenylyl-sulfate kinase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 586 bits (1511), Expect = 4.88e-211
Identity = 291/296 (98.31%), Postives = 295/296 (99.66%), Query Frame = 0


Query: 1   MVAIGGISLPRFIAPSLHRQSFSPPAIGFARFPNRIRLRSTVALLDEVEKTGSRAAIVNR 60
           MVAIGGISLPRF+APSLHRQSFSPPAIGFARFP+RIRLRSTVALLDEVEKTGSRAAIVNR
Sbjct: 1   MVAIGGISLPRFVAPSLHRQSFSPPAIGFARFPDRIRLRSTVALLDEVEKTGSRAAIVNR 60

Query: 61  KVDGLVKSDCETDFDATLGNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ 120
           KVDGLVKSDCETDFDATL NGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ
Sbjct: 61  KVDGLVKSDCETDFDATLSNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ 120

Query: 121 KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRIGEVAKLFANAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKG 240
           RRIGEVAKLFA+AGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKG
Sbjct: 181 RRIGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVMSYLEQKGFLQA 296
           LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKV+SYLEQKGFLQA
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVLSYLEQKGFLQA 296

[Close]

BLAST of Cp4.1LG14g04520 vs. NCBI nr
Match: XP_022985087.1 (adenylyl-sulfate kinase 3-like [Cucurbita maxima])

HSP 1 Score: 581 bits (1497), Expect = 6.65e-209
Identity = 289/296 (97.64%), Postives = 292/296 (98.65%), Query Frame = 0


Query: 1   MVAIGGISLPRFIAPSLHRQSFSPPAIGFARFPNRIRLRSTVALLDEVEKTGSRAAIVNR 60
           MVAIGGISLPRF+APSLHRQSFSPPAIGFARFPNRI  RSTVALLDEVEKTGSRAAIVNR
Sbjct: 1   MVAIGGISLPRFVAPSLHRQSFSPPAIGFARFPNRISYRSTVALLDEVEKTGSRAAIVNR 60

Query: 61  KVDGLVKSDCETDFDATLGNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ 120
           KVDGLVKSDCETDFDATLGNGHARNSGK LGVLSTVGNSTNIKWHECSVGKIERQSLLEQ
Sbjct: 61  KVDGLVKSDCETDFDATLGNGHARNSGKILGVLSTVGNSTNIKWHECSVGKIERQSLLEQ 120

Query: 121 KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRIGEVAKLFANAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKG 240
           +RIGEVAKLFA+AGVICIASLISPYRRDRDACRAILPDGYFIEVFMDV LEVCEARDAKG
Sbjct: 181 KRIGEVAKLFAHAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVMSYLEQKGFLQA 296
           LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVMSYLEQKGFLQA
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVMSYLEQKGFLQA 296

[Close]

BLAST of Cp4.1LG14g04520 vs. NCBI nr
Match: XP_022922441.1 (adenylyl-sulfate kinase 3-like [Cucurbita moschata] >XP_022922442.1 adenylyl-sulfate kinase 3-like [Cucurbita moschata])

HSP 1 Score: 580 bits (1495), Expect = 1.34e-208
Identity = 289/296 (97.64%), Postives = 291/296 (98.31%), Query Frame = 0


Query: 1   MVAIGGISLPRFIAPSLHRQSFSPPAIGFARFPNRIRLRSTVALLDEVEKTGSRAAIVNR 60
           MVAIGGISLPRF+APSLHRQSFSPPAIGFARF NRI  RSTVALLDEVEKTGSRAAIVNR
Sbjct: 1   MVAIGGISLPRFVAPSLHRQSFSPPAIGFARFQNRISFRSTVALLDEVEKTGSRAAIVNR 60

Query: 61  KVDGLVKSDCETDFDATLGNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ 120
           KVDGLVKSDCETDFDATL NGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ
Sbjct: 61  KVDGLVKSDCETDFDATLSNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ 120

Query: 121 KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRIGEVAKLFANAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKG 240
           RRIGEVAKLFA+AGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKG
Sbjct: 181 RRIGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVMSYLEQKGFLQA 296
           LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKV SYLEQKGFLQA
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVTSYLEQKGFLQA 296

[Close]

BLAST of Cp4.1LG14g04520 vs. NCBI nr
Match: XP_023521660.1 (adenylyl-sulfate kinase 3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023521661.1 adenylyl-sulfate kinase 3-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 540 bits (1391), Expect = 8.24e-193
Identity = 267/274 (97.45%), Postives = 271/274 (98.91%), Query Frame = 0


Query: 1   MVAIGGISLPRFIAPSLHRQSFSPPAIGFARFPNRIRLRSTVALLDEVEKTGSRAAIVNR 60
           MVAIGGISLPRF+APSLHRQSFSPPAIGFARFPNRIRLRSTVALLDEVEKTGSRAAIVNR
Sbjct: 1   MVAIGGISLPRFVAPSLHRQSFSPPAIGFARFPNRIRLRSTVALLDEVEKTGSRAAIVNR 60

Query: 61  KVDGLVKSDCETDFDATLGNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ 120
           KVDGLVKSDCETDFDATLGNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ
Sbjct: 61  KVDGLVKSDCETDFDATLGNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ 120

Query: 121 KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRIGEVAKLFANAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKG 240
           RRIGEVAKLFANAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKG
Sbjct: 181 RRIGEVAKLFANAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNG 274
           LYKLARAGKIKGFTGIDDPYEVPLNCE++  + G
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEVIPNSRG 274

[Close]

BLAST of Cp4.1LG14g04520 vs. ExPASy TrEMBL
Match: A0A6J1J3W8 (Adenylyl-sulfate kinase OS=Cucurbita maxima OX=3661 GN=LOC111483165 PE=3 SV=1)

HSP 1 Score: 581 bits (1497), Expect = 3.22e-209
Identity = 289/296 (97.64%), Postives = 292/296 (98.65%), Query Frame = 0


Query: 1   MVAIGGISLPRFIAPSLHRQSFSPPAIGFARFPNRIRLRSTVALLDEVEKTGSRAAIVNR 60
           MVAIGGISLPRF+APSLHRQSFSPPAIGFARFPNRI  RSTVALLDEVEKTGSRAAIVNR
Sbjct: 1   MVAIGGISLPRFVAPSLHRQSFSPPAIGFARFPNRISYRSTVALLDEVEKTGSRAAIVNR 60

Query: 61  KVDGLVKSDCETDFDATLGNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ 120
           KVDGLVKSDCETDFDATLGNGHARNSGK LGVLSTVGNSTNIKWHECSVGKIERQSLLEQ
Sbjct: 61  KVDGLVKSDCETDFDATLGNGHARNSGKILGVLSTVGNSTNIKWHECSVGKIERQSLLEQ 120

Query: 121 KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRIGEVAKLFANAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKG 240
           +RIGEVAKLFA+AGVICIASLISPYRRDRDACRAILPDGYFIEVFMDV LEVCEARDAKG
Sbjct: 181 KRIGEVAKLFAHAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVMSYLEQKGFLQA 296
           LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVMSYLEQKGFLQA
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVMSYLEQKGFLQA 296

[Close]

BLAST of Cp4.1LG14g04520 vs. ExPASy TrEMBL
Match: A0A6J1E450 (Adenylyl-sulfate kinase OS=Cucurbita moschata OX=3662 GN=LOC111430457 PE=3 SV=1)

HSP 1 Score: 580 bits (1495), Expect = 6.50e-209
Identity = 289/296 (97.64%), Postives = 291/296 (98.31%), Query Frame = 0


Query: 1   MVAIGGISLPRFIAPSLHRQSFSPPAIGFARFPNRIRLRSTVALLDEVEKTGSRAAIVNR 60
           MVAIGGISLPRF+APSLHRQSFSPPAIGFARF NRI  RSTVALLDEVEKTGSRAAIVNR
Sbjct: 1   MVAIGGISLPRFVAPSLHRQSFSPPAIGFARFQNRISFRSTVALLDEVEKTGSRAAIVNR 60

Query: 61  KVDGLVKSDCETDFDATLGNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ 120
           KVDGLVKSDCETDFDATL NGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ
Sbjct: 61  KVDGLVKSDCETDFDATLSNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ 120

Query: 121 KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRIGEVAKLFANAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKG 240
           RRIGEVAKLFA+AGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKG
Sbjct: 181 RRIGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVMSYLEQKGFLQA 296
           LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKV SYLEQKGFLQA
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVTSYLEQKGFLQA 296

[Close]

BLAST of Cp4.1LG14g04520 vs. ExPASy TrEMBL
Match: A0A6J1J8X7 (Adenylyl-sulfate kinase OS=Cucurbita maxima OX=3661 GN=LOC111482821 PE=3 SV=1)

HSP 1 Score: 500 bits (1287), Expect = 3.36e-177
Identity = 248/297 (83.50%), Postives = 264/297 (88.89%), Query Frame = 0


Query: 1   MVAIGGISLPRFIAPSLHRQSFSPP-AIGFARFPNRIRLRSTVALLDEVEKTGSRAAIVN 60
           MV+ GGIS P  + P L  +SFSPP A GFARF N I  +STV + D  EK GSRA IVN
Sbjct: 1   MVSTGGISFPGCVPPPLRHKSFSPPSAFGFARFSNGISFKSTVVVCDWAEKKGSRAVIVN 60

Query: 61  RKVDGLVKSDCETDFDATLGNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLE 120
            KVDGL KS+CE D DATLGNGHA NSGKN GVLSTVGNSTNIKWHECS+G +ERQSLL 
Sbjct: 61  GKVDGLGKSECEIDLDATLGNGHAGNSGKNAGVLSTVGNSTNIKWHECSIGNVERQSLLN 120

Query: 121 QKGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAEN 180
           QKGCVIWITGLSGSGKS VACAL + LYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAEN
Sbjct: 121 QKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAEN 180

Query: 181 IRRIGEVAKLFANAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAK 240
           IRR+GEVAKLFA+AGVICIASLISPYRRDRDACRAILPDGYFIEVFMDV LE+CEARD K
Sbjct: 181 IRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEICEARDTK 240

Query: 241 GLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVMSYLEQKGFLQA 296
           GLYKLARAGKIKGFTGIDDPYEVPLNCEIVLK+NGGSPC+MA+KVMSYLEQKGFL+A
Sbjct: 241 GLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMSYLEQKGFLKA 297

[Close]

BLAST of Cp4.1LG14g04520 vs. ExPASy TrEMBL
Match: A0A1S3BYH9 (Adenylyl-sulfate kinase OS=Cucumis melo OX=3656 GN=LOC103494812 PE=3 SV=1)

HSP 1 Score: 499 bits (1286), Expect = 4.61e-177
Identity = 251/296 (84.80%), Postives = 265/296 (89.53%), Query Frame = 0


Query: 1   MVAIGGISLPRFIAPSLHRQSFSPPAIGFARFPNRIRLRSTVALLDEVEKTGSRAAIVNR 60
           MV+IG ISLP  + PSLH Q   P AIGFARFP+RI  RS+V L DEVEK GSR  IVN 
Sbjct: 1   MVSIGRISLPGSVPPSLHHQCSPPSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG 60

Query: 61  KVDGLVKSDCETDFDATLGNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ 120
           KVDGL KS+CET+FD  LGNG A NSGKN GVLSTVGNSTNIKWHECSVGK E+QSLL+Q
Sbjct: 61  KVDGLGKSECETEFDTALGNGRAGNSGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120

Query: 121 KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIW TGLSGSGKS VACAL+Q L KMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWFTGLSGSGKSTVACALSQSLCKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRIGEVAKLFANAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKG 240
           RR+GEVAKLFA+AGVICIASLISPYRRDRDACRAILPDGYFIEVFMDV LEVCEARDAKG
Sbjct: 181 RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVMSYLEQKGFLQA 296
           LYKLARAGKIKGFTGIDDPYEVPLNCEIVLK+ GGSP EMAEKV++YLEQKGFLQA
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHTGGSPSEMAEKVLNYLEQKGFLQA 296

[Close]

BLAST of Cp4.1LG14g04520 vs. ExPASy TrEMBL
Match: A0A5A7TN80 (Adenylyl-sulfate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00500 PE=3 SV=1)

HSP 1 Score: 499 bits (1286), Expect = 4.61e-177
Identity = 251/296 (84.80%), Postives = 265/296 (89.53%), Query Frame = 0


Query: 1   MVAIGGISLPRFIAPSLHRQSFSPPAIGFARFPNRIRLRSTVALLDEVEKTGSRAAIVNR 60
           MV+IG ISLP  + PSLH Q   P AIGFARFP+RI  RS+V L DEVEK GSR  IVN 
Sbjct: 1   MVSIGRISLPGSVPPSLHHQCSPPSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG 60

Query: 61  KVDGLVKSDCETDFDATLGNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ 120
           KVDGL KS+CET+FD  LGNG A NSGKN GVLSTVGNSTNIKWHECSVGK E+QSLL+Q
Sbjct: 61  KVDGLGKSECETEFDTALGNGRAGNSGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120

Query: 121 KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIW TGLSGSGKS VACAL+Q L KMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWFTGLSGSGKSTVACALSQSLCKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRIGEVAKLFANAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKG 240
           RR+GEVAKLFA+AGVICIASLISPYRRDRDACRAILPDGYFIEVFMDV LEVCEARDAKG
Sbjct: 181 RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVMSYLEQKGFLQA 296
           LYKLARAGKIKGFTGIDDPYEVPLNCEIVLK+ GGSP EMAEKV++YLEQKGFLQA
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHTGGSPSEMAEKVLNYLEQKGFLQA 296

[Close]

BLAST of Cp4.1LG14g04520 vs. TAIR 10
Match: AT2G14750.1 (APS kinase )

HSP 1 Score: 358.6 bits (919), Expect = 4.8e-99
Identity = 173/218 (79.36%), Postives = 192/218 (88.07%), Query Frame = 0


Query: 80  NGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQKGCVIWITGLSGSGKSCVA 139
           NGH   +G+  G LSTVGNSTNIKWHECSV K++RQ LL+QKGCVIW+TGLSGSGKS +A
Sbjct: 62  NGH---TGQKQGPLSTVGNSTNIKWHECSVEKVDRQRLLDQKGCVIWVTGLSGSGKSTLA 121

Query: 140 CALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRRIGEVAKLFANAGVICIA 199
           CALNQ+LY+ GKL YILDGDNVRHGLNRDL FKAEDRAENIRR+GEVAKLFA+AG+ICIA
Sbjct: 122 CALNQMLYQKGKLCYILDGDNVRHGLNRDLSFKAEDRAENIRRVGEVAKLFADAGIICIA 181

Query: 200 SLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKGLYKLARAGKIKGFTGIDDP 259
           SLISPYR DRDACR++LP+G F+EVFMDV L VCEARD KGLYKLARAGKIKGFTGIDDP
Sbjct: 182 SLISPYRTDRDACRSLLPEGDFVEVFMDVPLSVCEARDPKGLYKLARAGKIKGFTGIDDP 241

Query: 260 YEVPLNCEIVLKNNGG-SPCEMAEKVMSYLEQKGFLQA 297
           YE PLNCEI L   GG SP EMAEKV+ YL+ KG+LQA
Sbjct: 242 YEPPLNCEISLGREGGTSPIEMAEKVVGYLDNKGYLQA 276

[Close]

BLAST of Cp4.1LG14g04520 vs. TAIR 10
Match: AT3G03900.1 (adenosine-5'-phosphosulfate (APS) kinase 3 )

HSP 1 Score: 320.5 bits (820), Expect = 1.4e-87
Identity = 154/206 (74.76%), Postives = 177/206 (85.92%), Query Frame = 0


Query: 93  LSTVGNSTNIKWHECSVGKIERQSLLEQKGCVIWITGLSGSGKSCVACALNQILYKMGKL 152
           +STVGNSTNI W E  +GK ERQ LL QKGCV+WITGLSGSGKS +AC+L++ L   GKL
Sbjct: 1   MSTVGNSTNIFWQESPIGKTERQKLLNQKGCVVWITGLSGSGKSTLACSLSRELNNRGKL 60

Query: 153 AYILDGDNVRHGLNRDLGFKAEDRAENIRRIGEVAKLFANAGVICIASLISPYRRDRDAC 212
           +YILDGDN+RHGLN+DLGFKAEDR ENIRR+GEVAKLFA+AG+ICIASLISPYR+DRDAC
Sbjct: 61  SYILDGDNLRHGLNKDLGFKAEDRVENIRRVGEVAKLFADAGLICIASLISPYRKDRDAC 120

Query: 213 RAILPDGYFIEVFMDVSLEVCEARDAKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKN 272
           R ++ +  FIEVFM++SL++CEARD KGLYKLARAGKIKGFTGIDDPYE PLNCEI LK 
Sbjct: 121 REMIQNSSFIEVFMNMSLQLCEARDPKGLYKLARAGKIKGFTGIDDPYESPLNCEIELKE 180

Query: 273 NGG---SPCEMAEKVMSYLEQKGFLQ 296
             G   SP  MAE+V+SYLE KGFLQ
Sbjct: 181 KEGECPSPVAMAEEVISYLEDKGFLQ 206

[Close]

BLAST of Cp4.1LG14g04520 vs. TAIR 10
Match: AT4G39940.1 (APS-kinase 2 )

HSP 1 Score: 315.5 bits (807), Expect = 4.7e-86
Identity = 164/276 (59.42%), Postives = 197/276 (71.38%), Query Frame = 0


Query: 24  PPAIGFARFPNRIRLRSTVALLDEVEKTGSRAAIVNRKVDGLVKSDCETDFDATLGNGHA 83
           PP+ GF + P       +  L+         +A VN      + +D     +  L  G  
Sbjct: 28  PPSDGFLKLPASSIPADSRKLVANSTSFHPISA-VNVSAQASLTADFPALSETILKEG-- 87

Query: 84  RNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQKGCVIWITGLSGSGKSCVACALN 143
           RN+GK          + NI WHE S+ + +RQ LL+QKGCV+WITGLSGSGKS VACAL+
Sbjct: 88  RNNGKE--------KAENIVWHESSICRCDRQQLLQQKGCVVWITGLSGSGKSTVACALS 147

Query: 144 QILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRRIGEVAKLFANAGVICIASLIS 203
           + L++ GKL Y LDGDNVRHGLNRDL FKAE R ENIRRIGEVAKLFA+ GVICIASLIS
Sbjct: 148 KALFERGKLTYTLDGDNVRHGLNRDLTFKAEHRTENIRRIGEVAKLFADVGVICIASLIS 207

Query: 204 PYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKGLYKLARAGKIKGFTGIDDPYEVP 263
           PYRRDRDACR++LPDG F+EVFMDV L VCE+RD KGLYKLARAGKIKGFTGIDDPYE P
Sbjct: 208 PYRRDRDACRSLLPDGDFVEVFMDVPLHVCESRDPKGLYKLARAGKIKGFTGIDDPYEAP 267

Query: 264 LNCEIVLKNNGG----SPCEMAEKVMSYLEQKGFLQ 296
           +NCE+VLK+ G     SP +MAE ++SYL+ KG+L+
Sbjct: 268 VNCEVVLKHTGDDESCSPRQMAENIISYLQNKGYLE 292

[Close]

BLAST of Cp4.1LG14g04520 vs. TAIR 10
Match: AT5G67520.1 (adenosine-5'-phosphosulfate (APS) kinase 4 )

HSP 1 Score: 299.7 bits (766), Expect = 2.6e-81
Identity = 144/217 (66.36%), Postives = 171/217 (78.80%), Query Frame = 0


Query: 97  GNSTNIKWHECSVGKIERQSLLEQKGCVIWITGLSGSGKSCVACALNQILYKMGKLAYIL 156
           G   NI WH+C V K +RQ L++QKGCVIWITGLSGSGKS +ACAL++ L+  GKL+YIL
Sbjct: 84  GKQKNIVWHDCPVTKSDRQELIKQKGCVIWITGLSGSGKSSLACALSRALHNRGKLSYIL 143

Query: 157 DGDNVRHGLNRDLGFKAEDRAENIRRIGEVAKLFANAGVICIASLISPYRRDRDACRAIL 216
           DGDNVRHGLN DL F+A+DRAENIRR+GEVAKLFA++G+ICIASLISPYR +R ACRA+L
Sbjct: 144 DGDNVRHGLNSDLSFEADDRAENIRRVGEVAKLFADSGIICIASLISPYRIERAACRALL 203

Query: 217 PDGYFIEVFMDVSLEVCEARDAKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKN---- 276
           P G FIEVFMDV L VCEARD KGLYK ARAGKIKGFTG+DDPYE PL+CEIV++N    
Sbjct: 204 PQGDFIEVFMDVPLHVCEARDPKGLYKRARAGKIKGFTGVDDPYEAPLDCEIVIQNSRDK 263

Query: 277 --------------NGGSPCEMAEKVMSYLEQKGFLQ 296
                         +  S CEMA+ V+SYL+Q G+L+
Sbjct: 264 GLSSSSSSSSSPSSSSSSLCEMADIVVSYLDQNGYLK 300

The following BLAST results are available for this feature:

BLAST of Cp4.1LG14g04520 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q43295	6.7e-98	79.36	Adenylyl-sulfate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK1...	[more]
O49204	2.6e-89	68.98	Adenylyl-sulfate kinase, chloroplastic OS=Catharanthus roseus OX=4058 GN=AKN PE=...	[more]
Q9SRW7	2.0e-86	74.76	Adenylyl-sulfate kinase 3 OS=Arabidopsis thaliana OX=3702 GN=APK3 PE=1 SV=1	[more]
O49196	6.6e-85	59.42	Adenylyl-sulfate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK2...	[more]
Q84JF0	3.7e-80	66.36	Adenylyl-sulfate kinase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK4...	[more]

BLAST of Cp4.1LG14g04520 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023551902.1	4.36e-214	100.00	adenylyl-sulfate kinase 3-like [Cucurbita pepo subsp. pepo]	[more]
KAG6576911.1	4.88e-211	98.31	Adenylyl-sulfate kinase 1, chloroplastic, partial [Cucurbita argyrosperma subsp....	[more]
XP_022985087.1	6.65e-209	97.64	adenylyl-sulfate kinase 3-like [Cucurbita maxima]	[more]
XP_022922441.1	1.34e-208	97.64	adenylyl-sulfate kinase 3-like [Cucurbita moschata] >XP_022922442.1 adenylyl-sul...	[more]
XP_023521660.1	8.24e-193	97.45	adenylyl-sulfate kinase 3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_02352...	[more]

BLAST of Cp4.1LG14g04520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J3W8	3.22e-209	97.64	Adenylyl-sulfate kinase OS=Cucurbita maxima OX=3661 GN=LOC111483165 PE=3 SV=1	[more]
A0A6J1E450	6.50e-209	97.64	Adenylyl-sulfate kinase OS=Cucurbita moschata OX=3662 GN=LOC111430457 PE=3 SV=1	[more]
A0A6J1J8X7	3.36e-177	83.50	Adenylyl-sulfate kinase OS=Cucurbita maxima OX=3661 GN=LOC111482821 PE=3 SV=1	[more]
A0A1S3BYH9	4.61e-177	84.80	Adenylyl-sulfate kinase OS=Cucumis melo OX=3656 GN=LOC103494812 PE=3 SV=1	[more]
A0A5A7TN80	4.61e-177	84.80	Adenylyl-sulfate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]

BLAST of Cp4.1LG14g04520 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G14750.1	4.8e-99	79.36	APS kinase	[more]
AT3G03900.1	1.4e-87	74.76	adenosine-5'-phosphosulfate (APS) kinase 3	[more]
AT4G39940.1	4.7e-86	59.42	APS-kinase 2	[more]
AT5G67520.1	2.6e-81	66.36	adenosine-5'-phosphosulfate (APS) kinase 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 94..296 e-value: 4.1E-92 score: 309.6
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 120..291
IPR002891	Adenylyl-sulfate kinase	TIGRFAM	TIGR00455	TIGR00455	coord: 104..287 e-value: 4.7E-74 score: 246.0
IPR002891	Adenylyl-sulfate kinase	HAMAP	MF_00065	Adenylyl_sulf_kinase	coord: 120..292 score: 35.472073
IPR002891	Adenylyl-sulfate kinase	CDD	cd02027	APSK	coord: 124..271 e-value: 1.2847E-96 score: 279.363
None	No IPR available	PFAM	PF01583	APS_kinase	coord: 121..273 e-value: 1.6E-70 score: 236.1
None	No IPR available	PANTHER	PTHR11055:SF55	ADENYLYL-SULFATE KINASE 1, CHLOROPLASTIC	coord: 51..296
None	No IPR available	PANTHER	PTHR11055	BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASE	coord: 51..296

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG14g04520.1	Cp4.1LG14g04520.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070814	hydrogen sulfide biosynthetic process
biological_process	GO:0016310	phosphorylation
biological_process	GO:0000103	sulfate assimilation
molecular_function	GO:0004020	adenylylsulfate kinase activity
molecular_function	GO:0005524	ATP binding