Cp4.1LG14g00720 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG14g00720
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionSNARE associated Golgi protein family
LocationCp4.1LG14: 4189567 .. 4192103 (+)
RNA-Seq ExpressionCp4.1LG14g00720
SyntenyCp4.1LG14g00720
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCACAAATCAATTGAAGAACTCGGTAAAAATGAAAATAGCAGGCATGAAGCATGTGAGAACAATGAATATGCAAGGCTAGTCATAGCTAATGAAGCAGGTCCTTTGGAATCAGAGATTCTACAGCCTCAAGATAAACTGAAAAGCAAATCACCCTTGAAGCGGTGGATCAAAATTTCACTATGGTGCATTGTTAGTATTGTATTTCTTCTTGTTTTCTTCAAGTGGGGAGTGCCATTTCTTTTCGAGAAGGTAATTCCCATTAATTACATCACCATTTTTTCTGTTTATAAGCTATTTTTCAGCTGAACTGTAGAAAATTTAACCTGTCCCTGTTATTTATTTTGAGAACTTTTTATTTTTTATTCATTTCTACTGCTCATAGTACAGATATGCTTGCAAACTTATGCAACTAACTTCGATTTAATAATGAGTTTGAATGTGGAATAATCATATCTGCAACTTTTTTTTCGTTTCTGTTAAATTTCTTTATAATATTCGTATCTGAAAATCTTAATGTTGGTTCGTGATGGCATTCTTACTGGAAAAAGAAAGTAATTAGTAAATGTTTGCATCAGCTGTAAAGCATCAAAGCATCTTAGAGAATGCAAGTTTGGAGAGTGGGAGGATGAGGTTTTCCCCCCTCCCTTACATGTTTCTTTGGTCTACAAAATCTTTCCTTTCTATAAAGGAATCTGCTACTGCATAAATGTTGATACAGAATCAGTTTAATTTTAATAAAAAAGTAACCAAGAGTGTAGTAAAATCTGCATATAGTGCATTGCTAGAGAGGATTTTGTGGTTTGTGTTCTTTTAGTATTGTTTGTAGGCACAAAATTAATATACTTTGAAATGGGACATCATCTTGATTATTGTCTGTATTATTGGGAGAACTTCAGTACTTGTTCTACAGTGGTCTCTAGAGCTTAAACTATTCTGATTGCAAATGCATGACCAAAATTATGTGATAAGGCAAAAAATTTGATGCAGAGAAGCAGTGATGAGCCGAGTGGTATCTTATTGTGAATTATGAATAAACGTACCGAATAATATTGTTCATTTGTCAATTAGAATACACATGACCTTTTTATGGTATTTTTAGTGTGTTTTGCTTTAAAAGTGTTCTATCGAATCTCTCTATCTCCAACGTCTGTACTTCCTATGATTTATTTGACCTCTATTTTTGCTCCCTGAGTTCATTTCAGGTCATCATCCCAATCATGAAGTGGGAAGCTACTGCCTTTCGTCGACCTATGCTTGCACTAATGCTTGTTGCTTCTCTGGCATCCTTTCCCGTATTTTTTATTCCCTCTGGCCCTTCAATGTGGTTGGCTGGGATGATTTTTGGCTATGGCCTTGGGTTTGTTATAATAATGGTTGGAACAACTATTGGGATGGTCCTGCCATATTTAATTGGATTAATTTTCCGTGACCGCATTCATGTAAGACCTCTATCAGTTTTTTTTTTTCAAATTATCGTGGCGGATAACTGGACCTATACTTTACAATCTTTCATTTGGTTCTCTGATAACTGGACCTATACTTTACAATCTTTCATTTGGTTCTCTGATCTCTGTATATTCATTATTGTGCAGCTATGGTTAATGAGATGGCCTCGAAAAGCAGAAGTACTCAGGCTTGCTGGGGAAGGGAGCTGGTTTCGTCAGTTTCAAGTTGTTGCACTCTTCAGGGTTTCACCATTTCCCTACACTATTTTCAATTATGCAATTGTGGTAACAAGTATGAGATTTTGGCCCTATTTGTGCGGGTCAGTTGCAGGAATGATACCAGAGGCTTTCATTTACATTTACAGGTTTCTTTCTTAGTCCCTCGAACGACTTACTAATAATCGAAAAGAAAAGTTAATATGCTGGTGTGCATGAATATATAGACAATAGGCCATTTTTTTTAGATTTACAAGAATACTTTCAGGAATTGTGTTTCTGTCACTTCATATGAAACTAGAACATGTCTTAATTTTCTTAGATCATTCATATGAAACAGGAAGAATCTCTATCGAGATAACGATAATGATGGGGTGCATTTTATGGTTAATTCATAGATATTGCTATAACCGGAGTTCTTTAGCTGGTAATGTAAGCTATCTCTAGATGGGACGTTCCGATTATTCAAGAAAACGATAACTGTAAACAATGTTAACGTTTATCTATTATCAATTCATACTGCCATAATGCTTGTGTGGATGTAACCTTGTTTTGCATAATCTGATATTTCCTTTCCCTAAACTTCTTTGCGCTCGTTAACGTCTGCTAAACATATCAGCTTTACTTATATGCAGTGGTAGGCTAATGAGGACACTAGCAGATGTTCAATATGGAAAACATCGGCTGACGACTGTAGAGATTGTGTATAACGTCATCTCCTTCATCATCGCGATCGTCACTATTATTATTTTCACTGTTTATGCAAAAAAGATGCTGAACAACCTTCAAATGGAAGAAGATAATAGAAAGTATTCTGCCTCCCACCATGGTAGTTTCGAGGTAGAGAGTCTTTCGCACGAAAGGTACAAGTAG

mRNA sequence

ATGCACAAATCAATTGAAGAACTCGGTAAAAATGAAAATAGCAGGCATGAAGCATGTGAGAACAATGAATATGCAAGGCTAGTCATAGCTAATGAAGCAGGTCCTTTGGAATCAGAGATTCTACAGCCTCAAGATAAACTGAAAAGCAAATCACCCTTGAAGCGGTGGATCAAAATTTCACTATGGTGCATTGTTAGTATTGTATTTCTTCTTGTTTTCTTCAAGTGGGGAGTGCCATTTCTTTTCGAGAAGGTCATCATCCCAATCATGAAGTGGGAAGCTACTGCCTTTCGTCGACCTATGCTTGCACTAATGCTTGTTGCTTCTCTGGCATCCTTTCCCGTATTTTTTATTCCCTCTGGCCCTTCAATGTGGTTGGCTGGGATGATTTTTGGCTATGGCCTTGGGTTTGTTATAATAATGGTTGGAACAACTATTGGGATGGTCCTGCCATATTTAATTGGATTAATTTTCCGTGACCGCATTCATCTATGGTTAATGAGATGGCCTCGAAAAGCAGAAGTACTCAGGCTTGCTGGGGAAGGGAGCTGGTTTCGTCAGTTTCAAGTTGTTGCACTCTTCAGGGTTTCACCATTTCCCTACACTATTTTCAATTATGCAATTGTGGTAACAAGTATGAGATTTTGGCCCTATTTGTGCGGGTCAGTTGCAGGAATGATACCAGAGGCTTTCATTTACATTTACAGTGGTAGGCTAATGAGGACACTAGCAGATGTTCAATATGGAAAACATCGGCTGACGACTGTAGAGATTGTGTATAACGTCATCTCCTTCATCATCGCGATCGTCACTATTATTATTTTCACTGTTTATGCAAAAAAGATGCTGAACAACCTTCAAATGGAAGAAGATAATAGAAAGTATTCTGCCTCCCACCATGGTAGTTTCGAGGTAGAGAGTCTTTCGCACGAAAGGTACAAGTAG

Coding sequence (CDS)

ATGCACAAATCAATTGAAGAACTCGGTAAAAATGAAAATAGCAGGCATGAAGCATGTGAGAACAATGAATATGCAAGGCTAGTCATAGCTAATGAAGCAGGTCCTTTGGAATCAGAGATTCTACAGCCTCAAGATAAACTGAAAAGCAAATCACCCTTGAAGCGGTGGATCAAAATTTCACTATGGTGCATTGTTAGTATTGTATTTCTTCTTGTTTTCTTCAAGTGGGGAGTGCCATTTCTTTTCGAGAAGGTCATCATCCCAATCATGAAGTGGGAAGCTACTGCCTTTCGTCGACCTATGCTTGCACTAATGCTTGTTGCTTCTCTGGCATCCTTTCCCGTATTTTTTATTCCCTCTGGCCCTTCAATGTGGTTGGCTGGGATGATTTTTGGCTATGGCCTTGGGTTTGTTATAATAATGGTTGGAACAACTATTGGGATGGTCCTGCCATATTTAATTGGATTAATTTTCCGTGACCGCATTCATCTATGGTTAATGAGATGGCCTCGAAAAGCAGAAGTACTCAGGCTTGCTGGGGAAGGGAGCTGGTTTCGTCAGTTTCAAGTTGTTGCACTCTTCAGGGTTTCACCATTTCCCTACACTATTTTCAATTATGCAATTGTGGTAACAAGTATGAGATTTTGGCCCTATTTGTGCGGGTCAGTTGCAGGAATGATACCAGAGGCTTTCATTTACATTTACAGTGGTAGGCTAATGAGGACACTAGCAGATGTTCAATATGGAAAACATCGGCTGACGACTGTAGAGATTGTGTATAACGTCATCTCCTTCATCATCGCGATCGTCACTATTATTATTTTCACTGTTTATGCAAAAAAGATGCTGAACAACCTTCAAATGGAAGAAGATAATAGAAAGTATTCTGCCTCCCACCATGGTAGTTTCGAGGTAGAGAGTCTTTCGCACGAAAGGTACAAGTAG

Protein sequence

MHKSIEELGKNENSRHEACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKISLWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLVASLASFPVFFIPSGPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAGEGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLMRTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHHGSFEVESLSHERYK
Homology
BLAST of Cp4.1LG14g00720 vs. ExPASy Swiss-Prot
Match: Q55909 (TVP38/TMEM64 family membrane protein slr0305 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr0305 PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 2.5e-10
Identity = 54/185 (29.19%), Postives = 94/185 (50.81%), Query Frame = 0

Query: 102 LALMLVASLASFPVFFIPSGPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDR 161
           +A ML+ ++A+  V F+P       AG++FG  LG + + +G T+G    +L+G   R  
Sbjct: 21  IAFMLLYTVAT--VVFLPGSILTLGAGVVFGVILGSIYVFIGATLGATAAFLVG---RYL 80

Query: 162 IHLWLMRWPRKAEVLRLAGEGSWFRQFQVVALFRVSP-FPYTIFNYAIVVTSMRFWPYLC 221
              W+ +     +  +   E       ++V L R+SP FP+ + NYA  +T++    Y+ 
Sbjct: 81  ARGWVAKKIAGNQKFKAIDEAVGKEGLKIVILTRLSPVFPFNLLNYAYGITNVSLKDYVI 140

Query: 222 GSVAGMIPEAFIYIYSGRLMRTLADVQYGKHRLT-TVEIVYNVISFIIAIVTIIIFTVYA 281
           GS+ GMIP   +Y+Y G L  +LA +    ++   T++    ++ FI  +   I  T  A
Sbjct: 141 GSL-GMIPGTIMYVYIGSLAGSLATLGTATNQANPTLQWTIRIVGFIATVAVTIYVTKIA 199

Query: 282 KKMLN 285
           +K LN
Sbjct: 201 RKALN 199

BLAST of Cp4.1LG14g00720 vs. ExPASy Swiss-Prot
Match: Q6YI46 (Transmembrane protein 64 OS=Homo sapiens OX=9606 GN=TMEM64 PE=2 SV=2)

HSP 1 Score: 52.4 bits (124), Expect = 1.1e-05
Identity = 40/142 (28.17%), Postives = 69/142 (48.59%), Query Frame = 0

Query: 116 FFIPSGPSMW-------LAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMR 175
           F + S P  W        AG ++G+ LG  ++MVG  IG    ++  ++ +  +  W+  
Sbjct: 167 FIVVSFPCGWGYIVLNVAAGYLYGFVLGMGLMMVGVLIG---TFIAHVVCKRLLTAWVAA 226

Query: 176 WPRKAE----VLRLAGEGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVA 235
             + +E    V+R+   GS  +   VVAL R++P P+ + N    +T +    YL  S  
Sbjct: 227 RIQSSEKLSAVIRVVEGGSGLK---VVALARLTPIPFGLQNAVFSITDLSLPNYLMASSV 286

Query: 236 GMIPEAFIYIYSGRLMRTLADV 247
           G++P   +  Y G  +RT+ DV
Sbjct: 287 GLLPTQLLNSYLGTTLRTMEDV 302

BLAST of Cp4.1LG14g00720 vs. ExPASy Swiss-Prot
Match: Q3U145 (Transmembrane protein 64 OS=Mus musculus OX=10090 GN=Tmem64 PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 4.2e-05
Identity = 38/139 (27.34%), Postives = 68/139 (48.92%), Query Frame = 0

Query: 116 FFIPSGPSMW-------LAGMIFGYGLGFVIIMVGTTIGMVLPYLI-GLIFRDRIHLWLM 175
           F + S P  W        AG ++G+ LG  +++VG  IG  + +++   +    +   + 
Sbjct: 168 FIVVSFPCGWGYIVLNVAAGYLYGFVLGMGLMVVGVLIGTFIAHVVCKRLLTAWVAARIQ 227

Query: 176 RWPRKAEVLRLAGEGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMI 235
              + + V+R+   GS  +   VVAL R++P P+ + N    +T +    YL  S AG++
Sbjct: 228 NSDKLSAVIRVVEGGSGLK---VVALARLTPIPFGLQNAVFSITDVPLPSYLMASSAGLL 287

Query: 236 PEAFIYIYSGRLMRTLADV 247
           P   +  Y G  +RT+ DV
Sbjct: 288 PTQLLNSYLGTTLRTMEDV 303

BLAST of Cp4.1LG14g00720 vs. NCBI nr
Match: XP_023552048.1 (uncharacterized protein LOC111809841 isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 606 bits (1562), Expect = 9.05e-218
Identity = 312/312 (100.00%), Postives = 312/312 (100.00%), Query Frame = 0

Query: 1   MHKSIEELGKNENSRHEACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS 60
           MHKSIEELGKNENSRHEACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS
Sbjct: 1   MHKSIEELGKNENSRHEACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS 60

Query: 61  LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLVASLASFPVFFIPS 120
           LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLVASLASFPVFFIPS
Sbjct: 61  LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLVASLASFPVFFIPS 120

Query: 121 GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG 180
           GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG
Sbjct: 121 GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG 180

Query: 181 EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM 240
           EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM
Sbjct: 181 EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM 240

Query: 241 RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH 300
           RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH
Sbjct: 241 RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH 300

Query: 301 GSFEVESLSHER 312
           GSFEVESLSHER
Sbjct: 301 GSFEVESLSHER 312

BLAST of Cp4.1LG14g00720 vs. NCBI nr
Match: XP_022929312.1 (uncharacterized protein LOC111435932 isoform X2 [Cucurbita moschata])

HSP 1 Score: 603 bits (1554), Expect = 5.50e-217
Identity = 310/312 (99.36%), Postives = 312/312 (100.00%), Query Frame = 0

Query: 1   MHKSIEELGKNENSRHEACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS 60
           MHKSIEELGKNENSR+EACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS
Sbjct: 1   MHKSIEELGKNENSRNEACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS 60

Query: 61  LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLVASLASFPVFFIPS 120
           LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALML+ASLASFPVFFIPS
Sbjct: 61  LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLIASLASFPVFFIPS 120

Query: 121 GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG 180
           GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG
Sbjct: 121 GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG 180

Query: 181 EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM 240
           EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM
Sbjct: 181 EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM 240

Query: 241 RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH 300
           RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH
Sbjct: 241 RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH 300

Query: 301 GSFEVESLSHER 312
           GSFEVESLSHER
Sbjct: 301 GSFEVESLSHER 312

BLAST of Cp4.1LG14g00720 vs. NCBI nr
Match: XP_022929310.1 (uncharacterized protein LOC111435932 isoform X1 [Cucurbita moschata] >XP_022929311.1 uncharacterized protein LOC111435932 isoform X1 [Cucurbita moschata])

HSP 1 Score: 603 bits (1554), Expect = 1.50e-216
Identity = 310/312 (99.36%), Postives = 312/312 (100.00%), Query Frame = 0

Query: 1   MHKSIEELGKNENSRHEACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS 60
           MHKSIEELGKNENSR+EACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS
Sbjct: 1   MHKSIEELGKNENSRNEACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS 60

Query: 61  LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLVASLASFPVFFIPS 120
           LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALML+ASLASFPVFFIPS
Sbjct: 61  LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLIASLASFPVFFIPS 120

Query: 121 GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG 180
           GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG
Sbjct: 121 GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG 180

Query: 181 EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM 240
           EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM
Sbjct: 181 EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM 240

Query: 241 RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH 300
           RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH
Sbjct: 241 RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH 300

Query: 301 GSFEVESLSHER 312
           GSFEVESLSHER
Sbjct: 301 GSFEVESLSHER 312

BLAST of Cp4.1LG14g00720 vs. NCBI nr
Match: XP_023552047.1 (uncharacterized protein LOC111809841 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 606 bits (1562), Expect = 1.54e-216
Identity = 312/312 (100.00%), Postives = 312/312 (100.00%), Query Frame = 0

Query: 1   MHKSIEELGKNENSRHEACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS 60
           MHKSIEELGKNENSRHEACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS
Sbjct: 103 MHKSIEELGKNENSRHEACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS 162

Query: 61  LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLVASLASFPVFFIPS 120
           LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLVASLASFPVFFIPS
Sbjct: 163 LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLVASLASFPVFFIPS 222

Query: 121 GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG 180
           GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG
Sbjct: 223 GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG 282

Query: 181 EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM 240
           EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM
Sbjct: 283 EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM 342

Query: 241 RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH 300
           RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH
Sbjct: 343 RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH 402

Query: 301 GSFEVESLSHER 312
           GSFEVESLSHER
Sbjct: 403 GSFEVESLSHER 414

BLAST of Cp4.1LG14g00720 vs. NCBI nr
Match: XP_023552045.1 (uncharacterized protein LOC111809841 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 606 bits (1562), Expect = 3.81e-216
Identity = 312/312 (100.00%), Postives = 312/312 (100.00%), Query Frame = 0

Query: 1   MHKSIEELGKNENSRHEACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS 60
           MHKSIEELGKNENSRHEACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS
Sbjct: 103 MHKSIEELGKNENSRHEACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS 162

Query: 61  LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLVASLASFPVFFIPS 120
           LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLVASLASFPVFFIPS
Sbjct: 163 LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLVASLASFPVFFIPS 222

Query: 121 GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG 180
           GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG
Sbjct: 223 GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG 282

Query: 181 EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM 240
           EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM
Sbjct: 283 EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM 342

Query: 241 RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH 300
           RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH
Sbjct: 343 RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH 402

Query: 301 GSFEVESLSHER 312
           GSFEVESLSHER
Sbjct: 403 GSFEVESLSHER 414

BLAST of Cp4.1LG14g00720 vs. ExPASy TrEMBL
Match: A0A6J1EMG0 (uncharacterized protein LOC111435932 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111435932 PE=4 SV=1)

HSP 1 Score: 603 bits (1554), Expect = 2.67e-217
Identity = 310/312 (99.36%), Postives = 312/312 (100.00%), Query Frame = 0

Query: 1   MHKSIEELGKNENSRHEACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS 60
           MHKSIEELGKNENSR+EACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS
Sbjct: 1   MHKSIEELGKNENSRNEACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS 60

Query: 61  LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLVASLASFPVFFIPS 120
           LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALML+ASLASFPVFFIPS
Sbjct: 61  LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLIASLASFPVFFIPS 120

Query: 121 GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG 180
           GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG
Sbjct: 121 GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG 180

Query: 181 EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM 240
           EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM
Sbjct: 181 EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM 240

Query: 241 RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH 300
           RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH
Sbjct: 241 RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH 300

Query: 301 GSFEVESLSHER 312
           GSFEVESLSHER
Sbjct: 301 GSFEVESLSHER 312

BLAST of Cp4.1LG14g00720 vs. ExPASy TrEMBL
Match: A0A6J1EMS5 (uncharacterized protein LOC111435932 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435932 PE=4 SV=1)

HSP 1 Score: 603 bits (1554), Expect = 7.26e-217
Identity = 310/312 (99.36%), Postives = 312/312 (100.00%), Query Frame = 0

Query: 1   MHKSIEELGKNENSRHEACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS 60
           MHKSIEELGKNENSR+EACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS
Sbjct: 1   MHKSIEELGKNENSRNEACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS 60

Query: 61  LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLVASLASFPVFFIPS 120
           LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALML+ASLASFPVFFIPS
Sbjct: 61  LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLIASLASFPVFFIPS 120

Query: 121 GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG 180
           GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG
Sbjct: 121 GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG 180

Query: 181 EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM 240
           EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM
Sbjct: 181 EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM 240

Query: 241 RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH 300
           RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH
Sbjct: 241 RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH 300

Query: 301 GSFEVESLSHER 312
           GSFEVESLSHER
Sbjct: 301 GSFEVESLSHER 312

BLAST of Cp4.1LG14g00720 vs. ExPASy TrEMBL
Match: A0A6J1J4T0 (uncharacterized protein LOC111482628 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111482628 PE=4 SV=1)

HSP 1 Score: 597 bits (1539), Expect = 5.55e-215
Identity = 307/312 (98.40%), Postives = 311/312 (99.68%), Query Frame = 0

Query: 1   MHKSIEELGKNENSRHEACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS 60
           MHKSIEELGK+ENSRHEACENNEYARLVIANEAGPLESEILQPQDKLKSKS LKRWIKIS
Sbjct: 1   MHKSIEELGKSENSRHEACENNEYARLVIANEAGPLESEILQPQDKLKSKSLLKRWIKIS 60

Query: 61  LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLVASLASFPVFFIPS 120
           LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALML+ASLASFPVFFIPS
Sbjct: 61  LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLIASLASFPVFFIPS 120

Query: 121 GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG 180
           GPSMWLAGMIFGYGLGFVII+VGTTIGMVLPYLIGLIFRDRIHLWLMRWP+KAEVLRLAG
Sbjct: 121 GPSMWLAGMIFGYGLGFVIILVGTTIGMVLPYLIGLIFRDRIHLWLMRWPQKAEVLRLAG 180

Query: 181 EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM 240
           EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM
Sbjct: 181 EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM 240

Query: 241 RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH 300
           RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH
Sbjct: 241 RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH 300

Query: 301 GSFEVESLSHER 312
           GSFEVESLSHER
Sbjct: 301 GSFEVESLSHER 312

BLAST of Cp4.1LG14g00720 vs. ExPASy TrEMBL
Match: A0A6J1J880 (uncharacterized protein LOC111482628 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482628 PE=4 SV=1)

HSP 1 Score: 597 bits (1539), Expect = 6.44e-215
Identity = 307/312 (98.40%), Postives = 311/312 (99.68%), Query Frame = 0

Query: 1   MHKSIEELGKNENSRHEACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS 60
           MHKSIEELGK+ENSRHEACENNEYARLVIANEAGPLESEILQPQDKLKSKS LKRWIKIS
Sbjct: 1   MHKSIEELGKSENSRHEACENNEYARLVIANEAGPLESEILQPQDKLKSKSLLKRWIKIS 60

Query: 61  LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLVASLASFPVFFIPS 120
           LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALML+ASLASFPVFFIPS
Sbjct: 61  LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLIASLASFPVFFIPS 120

Query: 121 GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG 180
           GPSMWLAGMIFGYGLGFVII+VGTTIGMVLPYLIGLIFRDRIHLWLMRWP+KAEVLRLAG
Sbjct: 121 GPSMWLAGMIFGYGLGFVIILVGTTIGMVLPYLIGLIFRDRIHLWLMRWPQKAEVLRLAG 180

Query: 181 EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM 240
           EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM
Sbjct: 181 EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM 240

Query: 241 RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH 300
           RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH
Sbjct: 241 RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH 300

Query: 301 GSFEVESLSHER 312
           GSFEVESLSHER
Sbjct: 301 GSFEVESLSHER 312

BLAST of Cp4.1LG14g00720 vs. ExPASy TrEMBL
Match: A0A6J1JA06 (uncharacterized protein LOC111482628 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482628 PE=4 SV=1)

HSP 1 Score: 597 bits (1539), Expect = 1.40e-214
Identity = 307/312 (98.40%), Postives = 311/312 (99.68%), Query Frame = 0

Query: 1   MHKSIEELGKNENSRHEACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKIS 60
           MHKSIEELGK+ENSRHEACENNEYARLVIANEAGPLESEILQPQDKLKSKS LKRWIKIS
Sbjct: 1   MHKSIEELGKSENSRHEACENNEYARLVIANEAGPLESEILQPQDKLKSKSLLKRWIKIS 60

Query: 61  LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLVASLASFPVFFIPS 120
           LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALML+ASLASFPVFFIPS
Sbjct: 61  LWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLIASLASFPVFFIPS 120

Query: 121 GPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAG 180
           GPSMWLAGMIFGYGLGFVII+VGTTIGMVLPYLIGLIFRDRIHLWLMRWP+KAEVLRLAG
Sbjct: 121 GPSMWLAGMIFGYGLGFVIILVGTTIGMVLPYLIGLIFRDRIHLWLMRWPQKAEVLRLAG 180

Query: 181 EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM 240
           EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM
Sbjct: 181 EGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLM 240

Query: 241 RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH 300
           RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH
Sbjct: 241 RTLADVQYGKHRLTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEEDNRKYSASHH 300

Query: 301 GSFEVESLSHER 312
           GSFEVESLSHER
Sbjct: 301 GSFEVESLSHER 312

BLAST of Cp4.1LG14g00720 vs. TAIR 10
Match: AT2G02370.1 (SNARE associated Golgi protein family )

HSP 1 Score: 386.3 bits (991), Expect = 2.3e-107
Identity = 198/272 (72.79%), Postives = 228/272 (83.82%), Query Frame = 0

Query: 21  NNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKISLWCIVSIVFLLVFFKWGVPF 80
           +NEY RLV+A+EA P E+ +   Q +++SK     W+K    C V+++  LVF KWGVPF
Sbjct: 21  DNEYVRLVVAHEASPAETVLSLSQSEVQSKK-FMWWLKALGICAVALLLTLVFGKWGVPF 80

Query: 81  LFEKVIIPIMKWEATAFRRPMLALMLVASLASFPVFFIPSGPSMWLAGMIFGYGLGFVII 140
           +F+KV+IPI++WEATAF RPMLA++LV SLA FPVF IPSGPSMWLAGMIFGYGLGFVII
Sbjct: 81  VFQKVLIPILQWEATAFGRPMLAIVLVVSLALFPVFLIPSGPSMWLAGMIFGYGLGFVII 140

Query: 141 MVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAGEGSWFRQFQVVALFRVSPFP 200
           MVGTTIGMVLPYLIGL+FRDR+H WL RWPR+A VLRLA EGSWF QF+VVA+FRVSPFP
Sbjct: 141 MVGTTIGMVLPYLIGLMFRDRLHQWLKRWPRQAAVLRLAAEGSWFHQFRVVAIFRVSPFP 200

Query: 201 YTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLMRTLADVQYGKHRLTTVEIVY 260
           YTIFNYAIVVTSMRFWPY  GS+AGMIPEAFIYIYSGRL+RT ADVQYG  RLTTVEIVY
Sbjct: 201 YTIFNYAIVVTSMRFWPYFFGSIAGMIPEAFIYIYSGRLIRTFADVQYGHQRLTTVEIVY 260

Query: 261 NVISFIIAIVTIIIFTVYAKKMLNNLQMEEDN 293
           NVIS +IA+VT + FTVYAK+ L  LQ  E N
Sbjct: 261 NVISLVIAVVTTVAFTVYAKRALRELQNAEAN 291

BLAST of Cp4.1LG14g00720 vs. TAIR 10
Match: AT2G02370.2 (SNARE associated Golgi protein family )

HSP 1 Score: 386.3 bits (991), Expect = 2.3e-107
Identity = 198/272 (72.79%), Postives = 228/272 (83.82%), Query Frame = 0

Query: 21  NNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKISLWCIVSIVFLLVFFKWGVPF 80
           +NEY RLV+A+EA P E+ +   Q +++SK     W+K    C V+++  LVF KWGVPF
Sbjct: 21  DNEYVRLVVAHEASPAETVLSLSQSEVQSKK-FMWWLKALGICAVALLLTLVFGKWGVPF 80

Query: 81  LFEKVIIPIMKWEATAFRRPMLALMLVASLASFPVFFIPSGPSMWLAGMIFGYGLGFVII 140
           +F+KV+IPI++WEATAF RPMLA++LV SLA FPVF IPSGPSMWLAGMIFGYGLGFVII
Sbjct: 81  VFQKVLIPILQWEATAFGRPMLAIVLVVSLALFPVFLIPSGPSMWLAGMIFGYGLGFVII 140

Query: 141 MVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAGEGSWFRQFQVVALFRVSPFP 200
           MVGTTIGMVLPYLIGL+FRDR+H WL RWPR+A VLRLA EGSWF QF+VVA+FRVSPFP
Sbjct: 141 MVGTTIGMVLPYLIGLMFRDRLHQWLKRWPRQAAVLRLAAEGSWFHQFRVVAIFRVSPFP 200

Query: 201 YTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLMRTLADVQYGKHRLTTVEIVY 260
           YTIFNYAIVVTSMRFWPY  GS+AGMIPEAFIYIYSGRL+RT ADVQYG  RLTTVEIVY
Sbjct: 201 YTIFNYAIVVTSMRFWPYFFGSIAGMIPEAFIYIYSGRLIRTFADVQYGHQRLTTVEIVY 260

Query: 261 NVISFIIAIVTIIIFTVYAKKMLNNLQMEEDN 293
           NVIS +IA+VT + FTVYAK+ L  LQ  E N
Sbjct: 261 NVISLVIAVVTTVAFTVYAKRALRELQNAEAN 291

BLAST of Cp4.1LG14g00720 vs. TAIR 10
Match: AT1G12450.1 (SNARE associated Golgi protein family )

HSP 1 Score: 223.0 bits (567), Expect = 3.3e-58
Identity = 117/276 (42.39%), Postives = 175/276 (63.41%), Query Frame = 0

Query: 17  EACENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKI--SLWCIVSIVFLLVFF 76
           E  +N +Y +L   +      S     +  + S + +  W+K+   + C+ S+ F+++  
Sbjct: 20  EDSDNGDYLKLRGGSNEEDEGSSAESSRCPIGSVTSVWFWVKLISLVVCLGSLAFVII-- 79

Query: 77  KWGVPFLFEKVIIPIMKWEATAFRRPMLALMLVASLASFPVFFIPSGPSMWLAGMIFGYG 136
           KW  PFL EK +IP + W    F  P+L L+L AS+A FP   +PS PSMW+AG+ FGYG
Sbjct: 80  KWVGPFLIEKELIPFINWVRNTFSIPVLGLLLFASVALFPSILLPSSPSMWMAGLTFGYG 139

Query: 137 LGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAGEGSWFRQFQVVALF 196
            GF++I+   +IG+ LP+LIG +F  ++  WL ++P+KA +LR AGEG+WF QFQ V L 
Sbjct: 140 KGFLLILSAASIGVTLPFLIGHLFLHKMQEWLKKYPKKAAILRAAGEGTWFHQFQAVTLI 199

Query: 197 RVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLMRTLADVQYGKHRLT 256
           RVSPFPY I+NY  + T + + PY+ GS+ GM+PE F+ IY+G ++RTLA     +H L+
Sbjct: 200 RVSPFPYIIYNYCALATGVHYGPYILGSLVGMVPEIFVSIYTGIMLRTLAVASDTRHTLS 259

Query: 257 TVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEE 291
            VEIV NV+ F +     I+ T+YAKK L+ +Q E+
Sbjct: 260 VVEIVVNVLGFCVTASATIVCTIYAKKKLSAMQSED 293

BLAST of Cp4.1LG14g00720 vs. TAIR 10
Match: AT4G22850.1 (SNARE associated Golgi protein family )

HSP 1 Score: 208.4 bits (529), Expect = 8.5e-54
Identity = 110/273 (40.29%), Postives = 170/273 (62.27%), Query Frame = 0

Query: 20  ENNEYARLVIANEAGPLESEILQPQDKLKSKSPLKRWIKISLWCIVSIVFLLVFFKWGVP 79
           E   Y +L    E G  ESE  + ++K  S S    W+K+SL         +V + W  P
Sbjct: 22  EKGPYVKLSEVLEKGQ-ESEQEKEEEKTDS-SRFWFWVKLSLLFAFLAALAVVGYIWIGP 81

Query: 80  FLFEKVIIPIMKWEATAFRRPMLALMLVASLASFPVFFIPSGPSMWLAGMIFGYGLGFVI 139
            + +K +IP+++WE   F  P+  L++ AS+A FP   +PS PSMW+AGM FGYG GF++
Sbjct: 82  LIMDKELIPLIQWEIRTFTHPVCGLLVFASVAIFPTILLPSTPSMWIAGMTFGYGYGFLL 141

Query: 140 IMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEVLRLAGEGSWFRQFQVVALFRVSPF 199
           I+    +G+ LPY IG +F  +I  WL R+P +A VLR AGEG+W  QF +V L R+SPF
Sbjct: 142 IISAAAVGVSLPYFIGQLFCHKIQGWLERYPDQAAVLRAAGEGNWLHQFLLVTLIRISPF 201

Query: 200 PYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIYSGRLMRTLADVQYGKHR-LTTVEI 259
           PY ++NY  V T +++ PY+ GS+ GM+PE F+ IY+G L+RTLA+    + + L+  ++
Sbjct: 202 PYILYNYCSVATRVKYGPYITGSLLGMVPEVFVAIYTGILVRTLAEASSAEEQGLSVTQV 261

Query: 260 VYNVISFIIAIVTIIIFTVYAKKMLNNLQMEED 292
           + N++ F+  + T I+ T YAK+ L  ++ E++
Sbjct: 262 ILNILGFLATVATTILITKYAKRQLETMKKEDE 292

BLAST of Cp4.1LG14g00720 vs. TAIR 10
Match: AT4G12000.1 (SNARE associated Golgi protein family )

HSP 1 Score: 201.1 bits (510), Expect = 1.4e-51
Identity = 99/237 (41.77%), Postives = 153/237 (64.56%), Query Frame = 0

Query: 56  WIKISLWCIVSIVFLLVFFKWGVPFLFEKVIIPIMKWEATAFRRPMLALMLVASLASFPV 115
           WIK+ L+        L  +KW  P + +K +IP++KWE   F  P+  +++ AS++ FPV
Sbjct: 65  WIKLGLFFTFLTALGLAGYKWLYPLIMDKELIPLIKWEMETFTHPVCGILVFASVSLFPV 124

Query: 116 FFIPSGPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLIFRDRIHLWLMRWPRKAEV 175
             IP+ PSMW+AG+ FGY  G ++ +    IG+ LPY I  +F ++I  WL R+P +A +
Sbjct: 125 ILIPTTPSMWVAGITFGYFYGLLLTLPAVAIGVSLPYFISYLFLNKIQGWLERYPDQAAM 184

Query: 176 LRLAGEGSWFRQFQVVALFRVSPFPYTIFNYAIVVTSMRFWPYLCGSVAGMIPEAFIYIY 235
           LR AG GSWF QF+ V L R+SPFP+ ++NY  V T ++F PY+ GS+ GM PE F+ IY
Sbjct: 185 LRAAGGGSWFHQFRAVTLIRISPFPFAVYNYCAVATRVKFGPYMAGSLVGMAPEIFVAIY 244

Query: 236 SGRLMRTLADVQYGKHR-LTTVEIVYNVISFIIAIVTIIIFTVYAKKMLNNLQMEED 292
           +G L+RTLAD    + + L+ ++IV N+  F+  +VT ++ T YAK+ L  ++ E++
Sbjct: 245 TGILIRTLADASTAEQKGLSILQIVLNIFGFVATVVTTVLITKYAKRQLEVMKKEKE 301

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q559092.5e-1029.19TVP38/TMEM64 family membrane protein slr0305 OS=Synechocystis sp. (strain PCC 68... [more]
Q6YI461.1e-0528.17Transmembrane protein 64 OS=Homo sapiens OX=9606 GN=TMEM64 PE=2 SV=2[more]
Q3U1454.2e-0527.34Transmembrane protein 64 OS=Mus musculus OX=10090 GN=Tmem64 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_023552048.19.05e-218100.00uncharacterized protein LOC111809841 isoform X4 [Cucurbita pepo subsp. pepo][more]
XP_022929312.15.50e-21799.36uncharacterized protein LOC111435932 isoform X2 [Cucurbita moschata][more]
XP_022929310.11.50e-21699.36uncharacterized protein LOC111435932 isoform X1 [Cucurbita moschata] >XP_0229293... [more]
XP_023552047.11.54e-216100.00uncharacterized protein LOC111809841 isoform X3 [Cucurbita pepo subsp. pepo][more]
XP_023552045.13.81e-216100.00uncharacterized protein LOC111809841 isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1EMG02.67e-21799.36uncharacterized protein LOC111435932 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1EMS57.26e-21799.36uncharacterized protein LOC111435932 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1J4T05.55e-21598.40uncharacterized protein LOC111482628 isoform X3 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1J8806.44e-21598.40uncharacterized protein LOC111482628 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1JA061.40e-21498.40uncharacterized protein LOC111482628 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
AT2G02370.12.3e-10772.79SNARE associated Golgi protein family [more]
AT2G02370.22.3e-10772.79SNARE associated Golgi protein family [more]
AT1G12450.13.3e-5842.39SNARE associated Golgi protein family [more]
AT4G22850.18.5e-5440.29SNARE associated Golgi protein family [more]
AT4G12000.11.4e-5141.77SNARE associated Golgi protein family [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032816SNARE associated Golgi proteinPFAMPF09335SNARE_assoccoord: 118..238
e-value: 7.1E-19
score: 68.4
NoneNo IPR availablePANTHERPTHR46431:SF5EXPRESSED PROTEINcoord: 6..311
NoneNo IPR availablePANTHERPTHR46431EXPRESSED PROTEINcoord: 6..311

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG14g00720.1Cp4.1LG14g00720.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008810 cellulase activity