Cp4.1LG14g00070 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG14g00070
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionDihydroflavonol-4-reductase
LocationCp4.1LG14: 4692992 .. 4695651 (-)
RNA-Seq ExpressionCp4.1LG14g00070
SyntenyCp4.1LG14g00070
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAGAGTCATTACATCTATTCTATGTGCATATATACTTACATGCTTCCTCAGAAAAATCCCTAAGACCCAACCTATGATTACACCAATTCTGAATTTTGAATGAGAATAGAGATTCCAAGATTCCTCTTATTTGGATGCCATCCCTAAAAAAGAATCATCTTTATCAAGAACATTTTCAATTTCATCCCATTGGGAGGTAAATTAAGATAAATTAAAAAAGTTATATAAGGAAAGAAGTTTGGATGTAAAAAGTGAAATTTAATTGGGTTGTTTGTCTGTTGTACAGTATTTTATATTTTTTACAGAGGGAAGGCATACGTTTTGTGTGGGTTTGTTGGAGCTTTTATGGAATCTGCATCCAAATAAATAAATAGAAGAAAAGAAAAGAAAAGGGGTTTGGGTTTGGGCGGAGAAAGCAGAGAGATGGAAGAAGAAGGATCAGAAAGAGGAAGGGGAAGTCCAACAACGTACTGTGTCACTGGTGCCACAGGCTTCATCGGCTCTTCCTTGGTTAACCTTCTTCTTCAAAATGGGTATAACGTCCATGCCACTCTTCGACACCCAGGTTTGTCACCTTCTTTTCTTTTTCTCTGCCTTTTTCTCTTTTCTTTTCTTTTTTTTTTTTTTTTCTTTTTCTTTTTTTATGATGGGACTAATTGTTTTTATCTACTTGACTCTTAGAAAATTTGTTGTAAATCTCGAAACTTCAAGAAACTGTAATACATACTCGAACTTTCAATTTTAGAAACAATTCATGTAATTATTAGACTGAAACCTTTTTTTTTTTTAATTAATCAAATTTTCAAGTTCACTTTCGGTGGTTTCATACTTTTTAAATATAAAATTGAAAATTTTGATAATAGAAAAAGAAGATGTAGCGATCATCAAATTAATGGAATGATATGATCAGCCCGAATTTTGATCTATAAATAAAGTAGATCGATTCTTTGTTCACATATTATATGATATAGAAGATGATGAATTTCTTGATCCAGTTGAACCATCAAATCCTAGTTTTAGGTTGGATATCGAGTGCATTATATGTTTAAGATATTTATTTTTTTTACTAGAATAGTCAAAATTTTGAATAACAACGTCACATCTAATTATGTTGTTCAAAATAAATTTTTAAGTGTCGTTAAGTACTTAATAAAATCTTTTTGTCATCAAATGGTGCAGCAAAGTCTTTAAAGCTATTATCTTTATGGACGAACACGGACCGTTTACAACTGTTCAAAGCCGATCTACGAGAAGAAGGAAGCTTTGATGAAGCTGTAAAAGGGTGTGATGGTGTATTTCATGTTGCTGCTTCAATGACTTTCAATGTTGATCAACAAGACAATATTGGTAAGCTCTTTTAATATAAGAAGATGAGATAATTGAATGAAGCAAAGACTTATGTTTTCCTCTTAATAACTTTCGAAGAGGAATATGTTCAAACAAATGTGATCAACCCGGAAATTAAGGGGACCATCAACGTGCTTAAGTCATGCATGAAATCTAGATCAGTGAAGCGAGTCGTTCTCACGTCGACGATTAGTACTCTTACTGGTAAAGATGCAAATGGAAAACGAAAGCGTTTTGTTGATGAATCATGCCAAACTTTTGTAGATCAAGTTTGGAAGAACAAACCAAGTGGCTGGGTAAGTGACGTATATCATTTGGCTAATTTGAAACTCTACTCTAACTCTAATTCTCTTTGGTTGTTATAGGTGTATGCACTTTCAAAGCGTCTAAGTGAGGACGCAGCATTCAAATTTGCATATGAAAATGGTATTGATATCGTTTCAGTAATTACAAGCACAGTTTCTGGTCCATTCCTCACTTCCAATGTCCCATCAAGCGTTCAAGTTCTCATGGCACCAATAACAAGTAAAAATCTTTCTACATTTTTTTTTTATTATTATTATTCAACTACATAGATAAGCTAGCCAAAACAACTTTGTAATGTCATTTTCTCTCGGGTGATTTGAAAATCTTAAAGTAAATTAAACGAAATGAAATGATGAACGTAATCAATTTCACAGGAGATCCCAACTTCCTTAGAATAGTATCAACGGTGAACGAAAGAATAGGATCAGTTGCAGTGGTTCACACCAAGGATATATGTAGAGCTCACATCTTCCTAATGGAGCACACGAAGGGCAAAGGTCGATATCTATGCTGTGTCGGAAGCTATACATTGTCAGAGTTGACGGAACGACTATCACAACATTGTGATGTGAAGTTGGAAAGGTAGTATTTGATTCGAGCAGCAAAATTGGATGTGGGGAGGTAGGGAAATTGACATGGTTTTGTTGTTTGTTGTGCAGATGTGTTGATGAGAAGCAAAATTGGATGCGTTCTGAGGTTGGAAACAAGAAATTGAAAGATTTGGGGTTCGAGTTTGAGCATGGAATAGATGATATAATAAAGGACACAGTTAGTTCGTGTGTTCAATGTGGCTTTATATCTCTTACTTAGAACCAATTTGGGTTTTGTATGGCTTACAATGAATCAAATTTTCTTTGGCCCAACTTCCGACAATGGACTTAGGTTAAATTGGCCTTTGGGCTAAGTCATGTTAAACCCAATTGACTCTTGACCTAATTATCACCCTTGGATTTGATCAAGCTTATTATTGTGATCAGATGACCATAAATAAAACATCGTTGACCGTG

mRNA sequence

TAGAGTCATTACATCTATTCTATGTGCATATATACTTACATGCTTCCTCAGAAAAATCCCTAAGACCCAACCTATGATTACACCAATTCTGAATTTTGAATGAGAATAGAGATTCCAAGATTCCTCTTATTTGGATGCCATCCCTAAAAAAGAATCATCTTTATCAAGAACATTTTCAATTTCATCCCATTGGGAGTATTTTATATTTTTTACAGAGGGAAGGCATACGTTTTGTGTGGGTTTGTTGGAGCTTTTATGGAATCTGCATCCAAATAAATAAATAGAAGAAAAGAAAAGAAAAGGGGTTTGGGTTTGGGCGGAGAAAGCAGAGAGATGGAAGAAGAAGGATCAGAAAGAGGAAGGGGAAGTCCAACAACGTACTGTGTCACTGGTGCCACAGGCTTCATCGGCTCTTCCTTGGTTAACCTTCTTCTTCAAAATGGGTATAACGTCCATGCCACTCTTCGACACCCAGAGGAATATGTTCAAACAAATGTGATCAACCCGGAAATTAAGGGGACCATCAACGTGCTTAAGTCATGCATGAAATCTAGATCAGTGAAGCGAGTCGTTCTCACGTCGACGATTAGTACTCTTACTGGTAAAGATGCAAATGGAAAACGAAAGCGTTTTGTTGATGAATCATGCCAAACTTTTGTAGATCAAGTTTGGAAGAACAAACCAAGTGGCTGGGTGTATGCACTTTCAAAGCGTCTAAGTGAGGACGCAGCATTCAAATTTGCATATGAAAATGGTATTGATATCGTTTCAGTAATTACAAGCACAGTTTCTGGTCCATTCCTCACTTCCAATGTCCCATCAAGCGTTCAAGTTCTCATGGCACCAATAACAAGAGATCCCAACTTCCTTAGAATAGTATCAACGGTGAACGAAAGAATAGGATCAGTTGCAGTGGTTCACACCAAGGATATATGTAGAGCTCACATCTTCCTAATGGAGCACACGAAGGGCAAAGGTCGATATCTATGCTGTGTCGGAAGCTATACATTGTCAGAGTTGACGGAACGACTATCACAACATTGTGATGTGAAGTTGGAAAGATGTGTTGATGAGAAGCAAAATTGGATGCGTTCTGAGGTTGGAAACAAGAAATTGAAAGATTTGGGGTTCGAGTTTGAGCATGGAATAGATGATATAATAAAGGACACAGTTAGTTCGTGTGTTCAATGTGGCTTTATATCTCTTACTTAGAACCAATTTGGGTTTTGTATGGCTTACAATGAATCAAATTTTCTTTGGCCCAACTTCCGACAATGGACTTAGGTTAAATTGGCCTTTGGGCTAAGTCATGTTAAACCCAATTGACTCTTGACCTAATTATCACCCTTGGATTTGATCAAGCTTATTATTGTGATCAGATGACCATAAATAAAACATCGTTGACCGTG

Coding sequence (CDS)

ATGGAAGAAGAAGGATCAGAAAGAGGAAGGGGAAGTCCAACAACGTACTGTGTCACTGGTGCCACAGGCTTCATCGGCTCTTCCTTGGTTAACCTTCTTCTTCAAAATGGGTATAACGTCCATGCCACTCTTCGACACCCAGAGGAATATGTTCAAACAAATGTGATCAACCCGGAAATTAAGGGGACCATCAACGTGCTTAAGTCATGCATGAAATCTAGATCAGTGAAGCGAGTCGTTCTCACGTCGACGATTAGTACTCTTACTGGTAAAGATGCAAATGGAAAACGAAAGCGTTTTGTTGATGAATCATGCCAAACTTTTGTAGATCAAGTTTGGAAGAACAAACCAAGTGGCTGGGTGTATGCACTTTCAAAGCGTCTAAGTGAGGACGCAGCATTCAAATTTGCATATGAAAATGGTATTGATATCGTTTCAGTAATTACAAGCACAGTTTCTGGTCCATTCCTCACTTCCAATGTCCCATCAAGCGTTCAAGTTCTCATGGCACCAATAACAAGAGATCCCAACTTCCTTAGAATAGTATCAACGGTGAACGAAAGAATAGGATCAGTTGCAGTGGTTCACACCAAGGATATATGTAGAGCTCACATCTTCCTAATGGAGCACACGAAGGGCAAAGGTCGATATCTATGCTGTGTCGGAAGCTATACATTGTCAGAGTTGACGGAACGACTATCACAACATTGTGATGTGAAGTTGGAAAGATGTGTTGATGAGAAGCAAAATTGGATGCGTTCTGAGGTTGGAAACAAGAAATTGAAAGATTTGGGGTTCGAGTTTGAGCATGGAATAGATGATATAATAAAGGACACAGTTAGTTCGTGTGTTCAATGTGGCTTTATATCTCTTACTTAG

Protein sequence

MEEEGSERGRGSPTTYCVTGATGFIGSSLVNLLLQNGYNVHATLRHPEEYVQTNVINPEIKGTINVLKSCMKSRSVKRVVLTSTISTLTGKDANGKRKRFVDESCQTFVDQVWKNKPSGWVYALSKRLSEDAAFKFAYENGIDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIVSTVNERIGSVAVVHTKDICRAHIFLMEHTKGKGRYLCCVGSYTLSELTERLSQHCDVKLERCVDEKQNWMRSEVGNKKLKDLGFEFEHGIDDIIKDTVSSCVQCGFISLT
Homology
BLAST of Cp4.1LG14g00070 vs. ExPASy Swiss-Prot
Match: Q5XLY0 (Putative anthocyanidin reductase OS=Ginkgo biloba OX=3311 PE=2 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.7e-45
Identity = 113/323 (34.98%), Postives = 175/323 (54.18%), Query Frame = 0

Query: 11  GSPTTYCVTGATGFIGSSLVNLLLQNGYNVHATLRHPE---------------------- 70
           G  TT CVTGA GF+ S LV  LL+ GY VHAT+R PE                      
Sbjct: 10  GQTTTVCVTGAAGFMASWLVKRLLEKGYIVHATVRDPENKAKVSHLLNLPGATDRLKLFR 69

Query: 71  --------------------------EYV----QTNVINPEIKGTINVLKSCMKSRSVKR 130
                                     E++    + ++I P I+GT+NVLKSC K  S+KR
Sbjct: 70  AELCEDGSFDAAVAGCNGVFHVATPTEFMPKDPENDLIKPAIEGTLNVLKSCTKVDSIKR 129

Query: 131 VVLTSTISTLTGKDANGKRKRFVDESCQTFVDQVWKNKPSGWVYALSKRLSEDAAFKFAY 190
           VV+TS+ +T++  +++ ++ +++DESC T V+ +   KP GW Y +SK L+E AA K+A 
Sbjct: 130 VVVTSSAATVSINNSS-EQNQYIDESCWTDVNFLTSQKPPGWAYPVSKTLAEQAALKYAE 189

Query: 191 ENGIDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIVSTVNERIGSVAVVHTK 250
           E+ +D+V+VI   V GP +T  VPSSV++ ++ IT D   +  +  +    GS+++VH  
Sbjct: 190 EHSLDVVTVIPVLVVGPAVTPTVPSSVELALSLITGDEFKMGALKGMQFVSGSISLVHID 249

Query: 251 DICRAHIFLMEHTKGKGRYLCCVGSYTLSELTERLS-QHCDVKLERCVDEKQNWMRSEVG 281
           D+C A IFLME    +GRY+C   +  + +L E LS ++   K+    D+     +  + 
Sbjct: 250 DVCSAQIFLMEKPSAQGRYICFPVNTGIPQLAEFLSKRYPQYKVPTKFDDVPATPKLTIS 309

BLAST of Cp4.1LG14g00070 vs. ExPASy Swiss-Prot
Match: Q5FB34 (Anthocyanidin reductase ((2S)-flavan-3-ol-forming) OS=Vitis vinifera OX=29760 GN=ANR PE=1 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 9.8e-41
Identity = 104/318 (32.70%), Postives = 166/318 (52.20%), Query Frame = 0

Query: 15  TYCVTGATGFIGSSLVNLLLQNGYNVHATLRHPE-------------------------- 74
           T CV G TGF+ S LV LLLQ GY V+ T+R P+                          
Sbjct: 11  TACVVGGTGFVASLLVKLLLQKGYAVNTTVRDPDNQKKVSHLLELQELGDLKIFRADLTD 70

Query: 75  -------------------------EYVQTNVINPEIKGTINVLKSCMKSRSVKRVVLTS 134
                                    E  + ++I P I+G +NV+K+C +++SVKRV+LTS
Sbjct: 71  ELSFEAPIAGCDFVFHVATPVHFASEDPENDMIKPAIQGVVNVMKACTRAKSVKRVILTS 130

Query: 135 TISTLTGKDANGKRKRFVDESCQTFVDQVWKNKPSGWVYALSKRLSEDAAFKFAYENGID 194
           + + +T    +G     VDE   T ++ +   KP  W Y  SK L+E AA+KFA EN ID
Sbjct: 131 SAAAVTINQLDG-TGLVVDEKNWTDIEFLTSAKPPTWGYPASKTLAEKAAWKFAEENNID 190

Query: 195 IVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIVSTVNERIGSVAVVHTKDICRA 254
           +++VI + ++G  LTS+VPSS+ + M+ IT +   +  +  +    GSV++ H +D+CRA
Sbjct: 191 LITVIPTLMAGSSLTSDVPSSIGLAMSLITGNEFLINGMKGMQMLSGSVSIAHVEDVCRA 250

Query: 255 HIFLMEHTKGKGRYLCCVGSYTLSELTERLS-QHCDVKLERCVDEKQNWMRSEVGNKKLK 281
           HIF+ E     GRY+CC  + ++ EL + LS ++   K+     +     +  + ++KL 
Sbjct: 251 HIFVAEKESASGRYICCAANTSVPELAKFLSKRYPQYKVPTDFGDFPPKSKLIISSEKLV 310

BLAST of Cp4.1LG14g00070 vs. ExPASy Swiss-Prot
Match: D7U6G6 (Anthocyanidin reductase ((2S)-flavan-3-ol-forming) OS=Vitis vinifera OX=29760 GN=ANR PE=3 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 9.8e-41
Identity = 103/318 (32.39%), Postives = 166/318 (52.20%), Query Frame = 0

Query: 15  TYCVTGATGFIGSSLVNLLLQNGYNVHATLRHPE-------------------------- 74
           T CV G TGF+ S LV LLLQ GY V+ T+R P+                          
Sbjct: 11  TACVVGGTGFVASLLVKLLLQKGYAVNTTVRDPDNQKKVSHLLELQELGDLKIFRADLTD 70

Query: 75  -------------------------EYVQTNVINPEIKGTINVLKSCMKSRSVKRVVLTS 134
                                    E  + ++I P ++G +NV+K+C +++SVKRV+LTS
Sbjct: 71  ELSFEAPIAGCDFVFHVATPVHFASEDPENDMIKPAVQGVVNVMKACTRAKSVKRVILTS 130

Query: 135 TISTLTGKDANGKRKRFVDESCQTFVDQVWKNKPSGWVYALSKRLSEDAAFKFAYENGID 194
           + + +T    +G     VDE   T ++ +   KP  W Y  SK L+E AA+KFA EN ID
Sbjct: 131 SAAAVTINQLDG-TGLVVDEKNWTDIEFLTSAKPPTWGYPASKTLAEKAAWKFAEENNID 190

Query: 195 IVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIVSTVNERIGSVAVVHTKDICRA 254
           +++VI + ++G  LTS+VPSS+ + M+ IT +   +  +  +    GSV++ H +D+CRA
Sbjct: 191 LITVIPTLMAGSSLTSDVPSSIGLAMSLITGNEFLINGMKGMQMLSGSVSIAHVEDVCRA 250

Query: 255 HIFLMEHTKGKGRYLCCVGSYTLSELTERLS-QHCDVKLERCVDEKQNWMRSEVGNKKLK 281
           HIF+ E     GRY+CC  + ++ EL + LS ++   K+     +  +  +  + + KL 
Sbjct: 251 HIFVAEKESASGRYICCAANTSVPELAKFLSKRYPQYKVPTDFGDFPSKSKLIISSDKLV 310

BLAST of Cp4.1LG14g00070 vs. ExPASy Swiss-Prot
Match: Q7PCC4 (Anthocyanidin reductase ((2S)-flavan-3-ol-forming) OS=Vitis vinifera OX=29760 GN=ANR PE=1 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 2.8e-40
Identity = 103/318 (32.39%), Postives = 166/318 (52.20%), Query Frame = 0

Query: 15  TYCVTGATGFIGSSLVNLLLQNGYNVHATLRHPE-------------------------- 74
           T CV G TGF+ S LV LLLQ GY V+ T+R P+                          
Sbjct: 11  TACVVGGTGFVASLLVKLLLQKGYAVNTTVRDPDNQKKVSHLLELQELGDLKIFRADLTD 70

Query: 75  -------------------------EYVQTNVINPEIKGTINVLKSCMKSRSVKRVVLTS 134
                                    E  + ++I P I+G +NV+K+C +++SVKRV+LTS
Sbjct: 71  ELSFEAPIAGCDFVFHVATPVHFASEDPENDMIKPAIQGVVNVMKACTRAKSVKRVILTS 130

Query: 135 TISTLTGKDANGKRKRFVDESCQTFVDQVWKNKPSGWVYALSKRLSEDAAFKFAYENGID 194
           + + +T    +G     VDE   T ++ +   KP  W Y  SK L+E AA+KFA EN ID
Sbjct: 131 SAAAVTINQLDG-TGLVVDEKNWTDIEFLTSAKPPTWGYPASKTLAEKAAWKFAEENNID 190

Query: 195 IVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIVSTVNERIGSVAVVHTKDICRA 254
           +++VI + ++G  LTS+VPSS+ + M+ IT +   +  +  +    GSV++ H +D+C+A
Sbjct: 191 LITVIPTLMAGSSLTSDVPSSIGLAMSLITGNEFLINGMKGMQMLSGSVSIAHVEDVCQA 250

Query: 255 HIFLMEHTKGKGRYLCCVGSYTLSELTERLS-QHCDVKLERCVDEKQNWMRSEVGNKKLK 281
           HIF+ E     GRY+CC  + ++ EL + LS ++   K+     +     +  + ++KL 
Sbjct: 251 HIFVAEKESASGRYICCAANTSVPELAKFLSKRYPQYKVPTDFGDFPPKSKLIISSEKLV 310

BLAST of Cp4.1LG14g00070 vs. ExPASy Swiss-Prot
Match: P51102 (Dihydroflavonol 4-reductase OS=Arabidopsis thaliana OX=3702 GN=DFRA PE=1 SV=2)

HSP 1 Score: 158.7 bits (400), Expect = 1.0e-37
Identity = 101/334 (30.24%), Postives = 168/334 (50.30%), Query Frame = 0

Query: 15  TYCVTGATGFIGSSLVNLLLQNGYNVHATLRHP--------------------------- 74
           T CVTGA+GFIGS LV  LL+ GY V AT+R P                           
Sbjct: 7   TVCVTGASGFIGSWLVMRLLERGYFVRATVRDPGNLKKVQHLLDLPNAKTLLTLWKADLS 66

Query: 75  -------------------------EEYVQTNVINPEIKGTINVLKSCMKSRSVKRVVLT 134
                                     +  +  VI P + G + ++K+C+K+++V+R V T
Sbjct: 67  EEGSYDDAINGCDGVFHVATPMDFESKDPENEVIKPTVNGMLGIMKACVKAKTVRRFVFT 126

Query: 135 STISTLTGKDANGKRKRFVDESCQTFVDQVWKNKPSGWVYALSKRLSEDAAFKFAYENGI 194
           S+  T+  ++    +K   DE+  + ++ +   K +GW+Y +SK L+E AA+ FA E G+
Sbjct: 127 SSAGTVNVEE---HQKNVYDENDWSDLEFIMSKKMTGWMYFVSKTLAEKAAWDFAEEKGL 186

Query: 195 DIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIVSTVNERIGSVAVVHTKDICR 254
           D +S+I + V GPF+T+++P S+   ++PITR+     I+     R G    VH  D+C 
Sbjct: 187 DFISIIPTLVVGPFITTSMPPSLITALSPITRNEAHYSII-----RQGQ--YVHLDDLCN 246

Query: 255 AHIFLMEHTKGKGRYLCCVGSYTLSELTE----RLSQHCDVKLERCVDEKQNWMRSEVGN 293
           AHIFL E    KGRY+C     T+  +++    +  ++        VDE  N    E  +
Sbjct: 247 AHIFLYEQAAAKGRYICSSHDATILTISKFLRPKYPEYNVPSTFEGVDE--NLKSIEFSS 306

BLAST of Cp4.1LG14g00070 vs. NCBI nr
Match: XP_023552763.1 (putative anthocyanidin reductase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 562 bits (1449), Expect = 7.97e-201
Identity = 292/348 (83.91%), Postives = 292/348 (83.91%), Query Frame = 0

Query: 1   MEEEGSERGRGSPTTYCVTGATGFIGSSLVNLLLQNGYNVHATLRHP------------- 60
           MEEEGSERGRGSPTTYCVTGATGFIGSSLVNLLLQNGYNVHATLRHP             
Sbjct: 1   MEEEGSERGRGSPTTYCVTGATGFIGSSLVNLLLQNGYNVHATLRHPAKSLKLLSLWTNT 60

Query: 61  -------------------------------------------EEYVQTNVINPEIKGTI 120
                                                      EEYVQTNVINPEIKGTI
Sbjct: 61  DRLQLFKADLREEGSFDEAVKGCDGVFHVAASMTFNVDQQDNIEEYVQTNVINPEIKGTI 120

Query: 121 NVLKSCMKSRSVKRVVLTSTISTLTGKDANGKRKRFVDESCQTFVDQVWKNKPSGWVYAL 180
           NVLKSCMKSRSVKRVVLTSTISTLTGKDANGKRKRFVDESCQTFVDQVWKNKPSGWVYAL
Sbjct: 121 NVLKSCMKSRSVKRVVLTSTISTLTGKDANGKRKRFVDESCQTFVDQVWKNKPSGWVYAL 180

Query: 181 SKRLSEDAAFKFAYENGIDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIVST 240
           SKRLSEDAAFKFAYENGIDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIVST
Sbjct: 181 SKRLSEDAAFKFAYENGIDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIVST 240

Query: 241 VNERIGSVAVVHTKDICRAHIFLMEHTKGKGRYLCCVGSYTLSELTERLSQHCDVKLERC 292
           VNERIGSVAVVHTKDICRAHIFLMEHTKGKGRYLCCVGSYTLSELTERLSQHCDVKLERC
Sbjct: 241 VNERIGSVAVVHTKDICRAHIFLMEHTKGKGRYLCCVGSYTLSELTERLSQHCDVKLERC 300

BLAST of Cp4.1LG14g00070 vs. NCBI nr
Match: XP_022985246.1 (putative anthocyanidin reductase [Cucurbita maxima])

HSP 1 Score: 527 bits (1358), Expect = 5.80e-187
Identity = 273/348 (78.45%), Postives = 282/348 (81.03%), Query Frame = 0

Query: 1   MEEEGSERGRGSPTTYCVTGATGFIGSSLVNLLLQNGYNVHATLRHP------------- 60
           MEE  S  GRGSPTTYCVTGATGFIGSSLVNLLLQNGY VHATLRHP             
Sbjct: 1   MEEAVSGGGRGSPTTYCVTGATGFIGSSLVNLLLQNGYKVHATLRHPAKSLKLLSLWTNT 60

Query: 61  -------------------------------------------EEYVQTNVINPEIKGTI 120
                                                      EEYVQTNVINPE+KGTI
Sbjct: 61  ERLQLFKADLREEGSFDEAIKGCDGVFHVAASMTFNVDQQDNIEEYVQTNVINPEVKGTI 120

Query: 121 NVLKSCMKSRSVKRVVLTSTISTLTGKDANGKRKRFVDESCQTFVDQVWKNKPSGWVYAL 180
           NV KSCMKSRSVKRVVLTSTISTLTGKD +GKR+RFVDESCQTFVDQVWKNKPSGWVYAL
Sbjct: 121 NVFKSCMKSRSVKRVVLTSTISTLTGKDVDGKRRRFVDESCQTFVDQVWKNKPSGWVYAL 180

Query: 181 SKRLSEDAAFKFAYENGIDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIVST 240
           SKRLSEDAAFKFA ENGIDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIVST
Sbjct: 181 SKRLSEDAAFKFASENGIDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIVST 240

Query: 241 VNERIGSVAVVHTKDICRAHIFLMEHTKGKGRYLCCVGSYTLSELTERLSQHCDVKLERC 292
           VNERIGSVAVVHT+DICRAHIFLMEHTK KGRYLCCVGSYTL ELT+RLS+HCDVKLERC
Sbjct: 241 VNERIGSVAVVHTRDICRAHIFLMEHTKAKGRYLCCVGSYTLLELTKRLSRHCDVKLERC 300

BLAST of Cp4.1LG14g00070 vs. NCBI nr
Match: KAG6577404.1 (hypothetical protein SDJN03_24978, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015482.1 hypothetical protein SDJN02_23118 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 526 bits (1356), Expect = 1.30e-186
Identity = 277/351 (78.92%), Postives = 286/351 (81.48%), Query Frame = 0

Query: 1   MEEEGSERGR--GSPTT-YCVTGATGFIGSSLVNLLLQNGYNVHATLRHP---------- 60
           MEE GSERGR  G+PTT YCVTGATGFI SSLVNLLLQ+GYNVHATLRHP          
Sbjct: 1   MEESGSERGRRRGTPTTTYCVTGATGFIASSLVNLLLQSGYNVHATLRHPAKSSKLLSLW 60

Query: 61  ----------------------------------------------EEYVQTNVINPEIK 120
                                                         EEYVQTNVINPEIK
Sbjct: 61  TNTDRLQLFKADLREEGSFDEAVKGCDGVFHVAASMTFNVDQQDNIEEYVQTNVINPEIK 120

Query: 121 GTINVLKSCMKSRSVKRVVLTSTISTLTGKDANGKRKRFVDESCQTFVDQVWKNKPSGWV 180
           GTINVLKSCMKSRSVKRVVLTSTISTLTGKDA+GKRKRFVDESCQTFVDQVWKNKPSGWV
Sbjct: 121 GTINVLKSCMKSRSVKRVVLTSTISTLTGKDADGKRKRFVDESCQTFVDQVWKNKPSGWV 180

Query: 181 YALSKRLSEDAAFKFAYENGIDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRI 240
           YALSKRLSEDAAFKFA +NGIDIVSVITSTVSGPFLTSNVPSSVQ+LMAPITRDPNFLRI
Sbjct: 181 YALSKRLSEDAAFKFASQNGIDIVSVITSTVSGPFLTSNVPSSVQLLMAPITRDPNFLRI 240

Query: 241 VSTVNERIGSVAVVHTKDICRAHIFLMEHTKGKGRYLCCVGSYTLSELTERLSQHCDVKL 292
           VSTVNERIGSVAVVHT DICRAH+FLMEHTK KGRYLCCVGSYTLSELTERLS+HC VKL
Sbjct: 241 VSTVNERIGSVAVVHTSDICRAHVFLMEHTKAKGRYLCCVGSYTLSELTERLSRHCHVKL 300

BLAST of Cp4.1LG14g00070 vs. NCBI nr
Match: XP_022929423.1 (putative anthocyanidin reductase [Cucurbita moschata])

HSP 1 Score: 510 bits (1314), Expect = 3.25e-180
Identity = 269/351 (76.64%), Postives = 281/351 (80.06%), Query Frame = 0

Query: 1   MEEEGSERGR--GSPTT-YCVTGATGFIGSSLVNLLLQNGYNVHATLRHP---------- 60
           MEE GSE GR  G+PTT YCVTGATGFI SSLVNLLLQ+GYNVHATLRHP          
Sbjct: 1   MEESGSEGGRRRGTPTTTYCVTGATGFIASSLVNLLLQSGYNVHATLRHPAKSLKLLSLW 60

Query: 61  ----------------------------------------------EEYVQTNVINPEIK 120
                                                         EEYVQTNVINPEIK
Sbjct: 61  MNTDRLQLFKADLREEGSFDEAVKGCDGVFHVAASMTFNVDQQDNIEEYVQTNVINPEIK 120

Query: 121 GTINVLKSCMKSRSVKRVVLTSTISTLTGKDANGKRKRFVDESCQTFVDQVWKNKPSGWV 180
           GT+NVLKSCMKSRSVKRVVLTSTISTLTGKD +GKR+ FVDESCQTFVDQVWKNKPSGWV
Sbjct: 121 GTLNVLKSCMKSRSVKRVVLTSTISTLTGKDVDGKRRCFVDESCQTFVDQVWKNKPSGWV 180

Query: 181 YALSKRLSEDAAFKFAYENGIDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRI 240
           YALSKRLSEDAAFKFA EN IDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFL+I
Sbjct: 181 YALSKRLSEDAAFKFASENDIDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLKI 240

Query: 241 VSTVNERIGSVAVVHTKDICRAHIFLMEHTKGKGRYLCCVGSYTLSELTERLSQHCDVKL 292
           +STVNERIGSVAVVHT DICRAHIFLME+TK KGRYLCCVGSYTLSELTERLS+HC VKL
Sbjct: 241 ISTVNERIGSVAVVHTSDICRAHIFLMENTKAKGRYLCCVGSYTLSELTERLSRHCHVKL 300

BLAST of Cp4.1LG14g00070 vs. NCBI nr
Match: XP_011650548.1 (putative anthocyanidin reductase [Cucumis sativus] >KAE8650161.1 hypothetical protein Csa_011461 [Cucumis sativus])

HSP 1 Score: 429 bits (1104), Expect = 3.14e-148
Identity = 227/350 (64.86%), Postives = 250/350 (71.43%), Query Frame = 0

Query: 3   EEGSERGRGS----PTTYCVTGATGFIGSSLVNLLLQNGYNVHATLRHP----------- 62
           EE  E   GS    P TYCVTGATGFIGS LVNLLL NGY VHAT R P           
Sbjct: 4   EEEDEENFGSKELGPKTYCVTGATGFIGSWLVNLLLLNGYKVHATARDPAKSLKLLSSWT 63

Query: 63  ---------------------------------------------EEYVQTNVINPEIKG 122
                                                        EEYVQ NVI+PEIKG
Sbjct: 64  VTDRLRLFKADLQEEGSFDEAVKGCDGVFHVAASMTFNVDQQDNIEEYVQKNVIDPEIKG 123

Query: 123 TINVLKSCMKSRSVKRVVLTSTISTLTGKDANGKRKRFVDESCQTFVDQVWKNKPSGWVY 182
           TIN+LKSC+KS+SVKRVVLTSTISTLTGKDA+G+R+R VDESC+T VDQVWKNKPSGWVY
Sbjct: 124 TINLLKSCLKSKSVKRVVLTSTISTLTGKDADGERRRLVDESCRTLVDQVWKNKPSGWVY 183

Query: 183 ALSKRLSEDAAFKFAYENGIDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIV 242
           +L KRLSEDAAFKFA EN IDIVS+ITSTVSGPFLTS +PSS++V  APIT D +FLRI+
Sbjct: 184 SLLKRLSEDAAFKFASENSIDIVSIITSTVSGPFLTSYIPSSIRVFTAPITGDSDFLRIL 243

Query: 243 STVNERIGSVAVVHTKDICRAHIFLMEHTKGKGRYLCCVGSYTLSELTERLSQHCDVKLE 292
           S VNER+GSVAVVHT DICRAHIFLMEH   KGRYLCCV S  LSEL ERLS+HC    +
Sbjct: 244 SNVNERMGSVAVVHTNDICRAHIFLMEHENAKGRYLCCVESCGLSELVERLSRHCGANFQ 303

BLAST of Cp4.1LG14g00070 vs. ExPASy TrEMBL
Match: A0A6J1J7L9 (putative anthocyanidin reductase OS=Cucurbita maxima OX=3661 GN=LOC111483297 PE=3 SV=1)

HSP 1 Score: 527 bits (1358), Expect = 2.81e-187
Identity = 273/348 (78.45%), Postives = 282/348 (81.03%), Query Frame = 0

Query: 1   MEEEGSERGRGSPTTYCVTGATGFIGSSLVNLLLQNGYNVHATLRHP------------- 60
           MEE  S  GRGSPTTYCVTGATGFIGSSLVNLLLQNGY VHATLRHP             
Sbjct: 1   MEEAVSGGGRGSPTTYCVTGATGFIGSSLVNLLLQNGYKVHATLRHPAKSLKLLSLWTNT 60

Query: 61  -------------------------------------------EEYVQTNVINPEIKGTI 120
                                                      EEYVQTNVINPE+KGTI
Sbjct: 61  ERLQLFKADLREEGSFDEAIKGCDGVFHVAASMTFNVDQQDNIEEYVQTNVINPEVKGTI 120

Query: 121 NVLKSCMKSRSVKRVVLTSTISTLTGKDANGKRKRFVDESCQTFVDQVWKNKPSGWVYAL 180
           NV KSCMKSRSVKRVVLTSTISTLTGKD +GKR+RFVDESCQTFVDQVWKNKPSGWVYAL
Sbjct: 121 NVFKSCMKSRSVKRVVLTSTISTLTGKDVDGKRRRFVDESCQTFVDQVWKNKPSGWVYAL 180

Query: 181 SKRLSEDAAFKFAYENGIDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIVST 240
           SKRLSEDAAFKFA ENGIDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIVST
Sbjct: 181 SKRLSEDAAFKFASENGIDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIVST 240

Query: 241 VNERIGSVAVVHTKDICRAHIFLMEHTKGKGRYLCCVGSYTLSELTERLSQHCDVKLERC 292
           VNERIGSVAVVHT+DICRAHIFLMEHTK KGRYLCCVGSYTL ELT+RLS+HCDVKLERC
Sbjct: 241 VNERIGSVAVVHTRDICRAHIFLMEHTKAKGRYLCCVGSYTLLELTKRLSRHCDVKLERC 300

BLAST of Cp4.1LG14g00070 vs. ExPASy TrEMBL
Match: A0A6J1ES23 (putative anthocyanidin reductase OS=Cucurbita moschata OX=3662 GN=LOC111435998 PE=3 SV=1)

HSP 1 Score: 510 bits (1314), Expect = 1.57e-180
Identity = 269/351 (76.64%), Postives = 281/351 (80.06%), Query Frame = 0

Query: 1   MEEEGSERGR--GSPTT-YCVTGATGFIGSSLVNLLLQNGYNVHATLRHP---------- 60
           MEE GSE GR  G+PTT YCVTGATGFI SSLVNLLLQ+GYNVHATLRHP          
Sbjct: 1   MEESGSEGGRRRGTPTTTYCVTGATGFIASSLVNLLLQSGYNVHATLRHPAKSLKLLSLW 60

Query: 61  ----------------------------------------------EEYVQTNVINPEIK 120
                                                         EEYVQTNVINPEIK
Sbjct: 61  MNTDRLQLFKADLREEGSFDEAVKGCDGVFHVAASMTFNVDQQDNIEEYVQTNVINPEIK 120

Query: 121 GTINVLKSCMKSRSVKRVVLTSTISTLTGKDANGKRKRFVDESCQTFVDQVWKNKPSGWV 180
           GT+NVLKSCMKSRSVKRVVLTSTISTLTGKD +GKR+ FVDESCQTFVDQVWKNKPSGWV
Sbjct: 121 GTLNVLKSCMKSRSVKRVVLTSTISTLTGKDVDGKRRCFVDESCQTFVDQVWKNKPSGWV 180

Query: 181 YALSKRLSEDAAFKFAYENGIDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRI 240
           YALSKRLSEDAAFKFA EN IDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFL+I
Sbjct: 181 YALSKRLSEDAAFKFASENDIDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLKI 240

Query: 241 VSTVNERIGSVAVVHTKDICRAHIFLMEHTKGKGRYLCCVGSYTLSELTERLSQHCDVKL 292
           +STVNERIGSVAVVHT DICRAHIFLME+TK KGRYLCCVGSYTLSELTERLS+HC VKL
Sbjct: 241 ISTVNERIGSVAVVHTSDICRAHIFLMENTKAKGRYLCCVGSYTLSELTERLSRHCHVKL 300

BLAST of Cp4.1LG14g00070 vs. ExPASy TrEMBL
Match: A0A6J1CP63 (putative anthocyanidin reductase OS=Momordica charantia OX=3673 GN=LOC111012896 PE=3 SV=1)

HSP 1 Score: 417 bits (1073), Expect = 8.14e-144
Identity = 221/343 (64.43%), Postives = 249/343 (72.59%), Query Frame = 0

Query: 7   ERGRGSPT-TYCVTGATGFIGSSLVNLLLQNGYNVHATLRHPE----------------- 66
           E GRGS + TYCVTGATGFIGS LV LLLQ GY VHATLR+PE                 
Sbjct: 12  EDGRGSKSATYCVTGATGFIGSWLVKLLLQRGYTVHATLRNPEKSLNLLSLWTGSDRLRL 71

Query: 67  ---------------------------------------EYVQTNVINPEIKGTINVLKS 126
                                                  +YVQ+NVINPEI GTI++LKS
Sbjct: 72  FRADLREEGSFDEAVKGCDGVFHVAASMTFSVDQQDNIEDYVQSNVINPEINGTISLLKS 131

Query: 127 CMKSRSVKRVVLTSTISTLTGKDANGKRKRFVDESCQTFVDQVWKNKPSGWVYALSKRLS 186
           C+KS SVKRVVLTSTISTLTGKDANG+R+  VDESC T +D VWK KPSGW+YA+SKRL+
Sbjct: 132 CLKSGSVKRVVLTSTISTLTGKDANGERRHVVDESCLTSMDHVWKTKPSGWIYAISKRLT 191

Query: 187 EDAAFKFAYENGIDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIVSTVNERI 246
           E+AAFKFAYENGIDIVSVITSTVSGPFL SN+P+SVQVL APIT DP FL IVS VNERI
Sbjct: 192 EEAAFKFAYENGIDIVSVITSTVSGPFLISNIPASVQVLTAPITGDPRFLGIVSNVNERI 251

Query: 247 GSVAVVHTKDICRAHIFLMEHTKGKGRYLCCVGSYTLSELTERLSQHCDVKLERCVDEKQ 292
           GSVA+VHT DICRAHIFLMEH   KGRYLCC+GS+ LS+L E LS+H    L+R VDEKQ
Sbjct: 252 GSVALVHTNDICRAHIFLMEHADAKGRYLCCIGSWPLSKLVECLSRHYPSILQRFVDEKQ 311

BLAST of Cp4.1LG14g00070 vs. ExPASy TrEMBL
Match: A0A1S3BSY6 (dihydroflavonol-4-reductase isoform X8 OS=Cucumis melo OX=3656 GN=LOC103493381 PE=3 SV=1)

HSP 1 Score: 416 bits (1070), Expect = 2.67e-143
Identity = 217/334 (64.97%), Postives = 241/334 (72.16%), Query Frame = 0

Query: 13  PTTYCVTGATGFIGSSLVNLLLQNGYNVHATLRHP------------------------- 72
           P TYCVTGATGFIGS LVNLLL NGY VHAT R P                         
Sbjct: 20  PKTYCVTGATGFIGSWLVNLLLLNGYKVHATARDPAKFLKLLSSWTVTDRLRLFKADLRE 79

Query: 73  -------------------------------EEYVQTNVINPEIKGTINVLKSCMKSRSV 132
                                          EEYV+ NVI+PEIKGTI++LKSC+KS SV
Sbjct: 80  EGSFDEAVKGCDGVFHVAASMIFNVDQQDNIEEYVRKNVIDPEIKGTISLLKSCLKSESV 139

Query: 133 KRVVLTSTISTLTGKDANGKRKRFVDESCQTFVDQVWKNKPSGWVYALSKRLSEDAAFKF 192
           KRVVLTSTISTLTGKDA+G+R+R VDESCQT VDQVWKNKPSGW+Y+L KRLSEDAAF F
Sbjct: 140 KRVVLTSTISTLTGKDADGERRRLVDESCQTSVDQVWKNKPSGWIYSLLKRLSEDAAFNF 199

Query: 193 AYENGIDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIVSTVNERIGSVAVVH 252
           A EN IDIVSVITSTVSGPFLTS +PSS++V  APIT D +FLRI+S VNER+GSVAVVH
Sbjct: 200 ASENSIDIVSVITSTVSGPFLTSYIPSSIRVFTAPITGDSDFLRILSNVNERMGSVAVVH 259

Query: 253 TKDICRAHIFLMEHTKGKGRYLCCVGSYTLSELTERLSQHCDVKLERCVDEKQNWMRSEV 290
           T DICRAHIFLMEH   KGRYLCCV S  LSEL +RLS+HC V  +R VDEK+NWM SEV
Sbjct: 260 TNDICRAHIFLMEHENAKGRYLCCVESCALSELVKRLSRHCAVNFQRSVDEKKNWMPSEV 319

BLAST of Cp4.1LG14g00070 vs. ExPASy TrEMBL
Match: A0A1S4DZ50 (dihydroflavonol-4-reductase isoform X7 OS=Cucumis melo OX=3656 GN=LOC103493381 PE=3 SV=1)

HSP 1 Score: 416 bits (1070), Expect = 3.78e-143
Identity = 217/334 (64.97%), Postives = 241/334 (72.16%), Query Frame = 0

Query: 13  PTTYCVTGATGFIGSSLVNLLLQNGYNVHATLRHP------------------------- 72
           P TYCVTGATGFIGS LVNLLL NGY VHAT R P                         
Sbjct: 30  PKTYCVTGATGFIGSWLVNLLLLNGYKVHATARDPAKFLKLLSSWTVTDRLRLFKADLRE 89

Query: 73  -------------------------------EEYVQTNVINPEIKGTINVLKSCMKSRSV 132
                                          EEYV+ NVI+PEIKGTI++LKSC+KS SV
Sbjct: 90  EGSFDEAVKGCDGVFHVAASMIFNVDQQDNIEEYVRKNVIDPEIKGTISLLKSCLKSESV 149

Query: 133 KRVVLTSTISTLTGKDANGKRKRFVDESCQTFVDQVWKNKPSGWVYALSKRLSEDAAFKF 192
           KRVVLTSTISTLTGKDA+G+R+R VDESCQT VDQVWKNKPSGW+Y+L KRLSEDAAF F
Sbjct: 150 KRVVLTSTISTLTGKDADGERRRLVDESCQTSVDQVWKNKPSGWIYSLLKRLSEDAAFNF 209

Query: 193 AYENGIDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIVSTVNERIGSVAVVH 252
           A EN IDIVSVITSTVSGPFLTS +PSS++V  APIT D +FLRI+S VNER+GSVAVVH
Sbjct: 210 ASENSIDIVSVITSTVSGPFLTSYIPSSIRVFTAPITGDSDFLRILSNVNERMGSVAVVH 269

Query: 253 TKDICRAHIFLMEHTKGKGRYLCCVGSYTLSELTERLSQHCDVKLERCVDEKQNWMRSEV 290
           T DICRAHIFLMEH   KGRYLCCV S  LSEL +RLS+HC V  +R VDEK+NWM SEV
Sbjct: 270 TNDICRAHIFLMEHENAKGRYLCCVESCALSELVKRLSRHCAVNFQRSVDEKKNWMPSEV 329

BLAST of Cp4.1LG14g00070 vs. TAIR 10
Match: AT4G27250.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 279.6 bits (714), Expect = 2.8e-75
Identity = 156/351 (44.44%), Postives = 212/351 (60.40%), Query Frame = 0

Query: 1   MEEEGSERGRGSPTTYCVTGATGFIGSSLVNLLLQNGYNVHATLR--------------- 60
           ME +G E       TYCVTGA+G+IGS LV  LLQ GY VHATLR               
Sbjct: 1   MELQGEE---SKTATYCVTGASGYIGSWLVKSLLQRGYTVHATLRDLAKSEYFQSKWKEN 60

Query: 61  ------------------------------------------HPEEYVQTNVINPEIKGT 120
                                                     + E YVQ+ VI P +KG 
Sbjct: 61  ERLRLFRADLRDDGSFDDAVKGCDGVFHVAASMEFDISSDHVNLESYVQSKVIEPALKGV 120

Query: 121 INVLKSCMKSRSVKRVVLTSTISTLTGKDANGKRKRFVDESCQTFVDQVWKNKPSGWVYA 180
            NVL SC+KS+SVKRVV TS+ISTLT KD N + + FVDE+C+  VD V K + SGW+Y 
Sbjct: 121 RNVLSSCLKSKSVKRVVFTSSISTLTAKDENERMRSFVDETCKAHVDHVLKTQASGWIYV 180

Query: 181 LSKRLSEDAAFKFAYENGIDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIVS 240
           LSK +SE+ AF++A E G+D+VSVIT+TVSGPFLT  VPSSVQVL++PIT D     I+S
Sbjct: 181 LSKLVSEEEAFRYAKERGMDLVSVITTTVSGPFLTPFVPSSVQVLLSPITGDSKLFAILS 240

Query: 241 TVNERIGSVAVVHTKDICRAHIFLMEHTKGKGRYLCCVGSYTLSEL-TERLSQH--CDVK 292
            VN+R+GS+A+VH +DICRAH+FLME  K KG+Y+CCV +  + EL     S+   C V+
Sbjct: 241 AVNKRMGSIALVHIEDICRAHLFLMEQPKAKGQYICCVDNIDMHELMLHHFSKDYLCKVQ 300

BLAST of Cp4.1LG14g00070 vs. TAIR 10
Match: AT4G27250.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 266.9 bits (681), Expect = 1.9e-71
Identity = 153/351 (43.59%), Postives = 208/351 (59.26%), Query Frame = 0

Query: 1   MEEEGSERGRGSPTTYCVTGATGFIGSSLVNLLLQNGYNVHATLR--------------- 60
           ME +G E       TYCVTGA+G+IGS LV  LLQ GY VHATLR               
Sbjct: 1   MELQGEE---SKTATYCVTGASGYIGSWLVKSLLQRGYTVHATLRDLAKSEYFQSKWKEN 60

Query: 61  ------------------------------------------HPEEYVQTNVINPEIKGT 120
                                                     + E YVQ+ VI P +KG 
Sbjct: 61  ERLRLFRADLRDDGSFDDAVKGCDGVFHVAASMEFDISSDHVNLESYVQSKVIEPALKGV 120

Query: 121 INVLKSCMKSRSVKRVVLTSTISTLTGKDANGKRKRFVDESCQTFVDQVWKNKPSGWVYA 180
            NVL SC+KS+SVKRVV TS+ISTLT KD N + + FVDE+C+  VD V K + SGW+Y 
Sbjct: 121 RNVLSSCLKSKSVKRVVFTSSISTLTAKDENERMRSFVDETCKAHVDHVLKTQASGWIYV 180

Query: 181 LSKRLSEDAAFKFAYENGIDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIVS 240
           LSK +SE+ AF++A E G+D+VSVIT+TVSGPFLT  VPSSVQVL++PIT          
Sbjct: 181 LSKLVSEEEAFRYAKERGMDLVSVITTTVSGPFLTPFVPSSVQVLLSPIT---------- 240

Query: 241 TVNERIGSVAVVHTKDICRAHIFLMEHTKGKGRYLCCVGSYTLSEL-TERLSQH--CDVK 292
            VN+R+GS+A+VH +DICRAH+FLME  K KG+Y+CCV +  + EL     S+   C V+
Sbjct: 241 AVNKRMGSIALVHIEDICRAHLFLMEQPKAKGQYICCVDNIDMHELMLHHFSKDYLCKVQ 300

BLAST of Cp4.1LG14g00070 vs. TAIR 10
Match: AT5G42800.1 (dihydroflavonol 4-reductase )

HSP 1 Score: 158.7 bits (400), Expect = 7.2e-39
Identity = 101/334 (30.24%), Postives = 168/334 (50.30%), Query Frame = 0

Query: 15  TYCVTGATGFIGSSLVNLLLQNGYNVHATLRHP--------------------------- 74
           T CVTGA+GFIGS LV  LL+ GY V AT+R P                           
Sbjct: 7   TVCVTGASGFIGSWLVMRLLERGYFVRATVRDPGNLKKVQHLLDLPNAKTLLTLWKADLS 66

Query: 75  -------------------------EEYVQTNVINPEIKGTINVLKSCMKSRSVKRVVLT 134
                                     +  +  VI P + G + ++K+C+K+++V+R V T
Sbjct: 67  EEGSYDDAINGCDGVFHVATPMDFESKDPENEVIKPTVNGMLGIMKACVKAKTVRRFVFT 126

Query: 135 STISTLTGKDANGKRKRFVDESCQTFVDQVWKNKPSGWVYALSKRLSEDAAFKFAYENGI 194
           S+  T+  ++    +K   DE+  + ++ +   K +GW+Y +SK L+E AA+ FA E G+
Sbjct: 127 SSAGTVNVEE---HQKNVYDENDWSDLEFIMSKKMTGWMYFVSKTLAEKAAWDFAEEKGL 186

Query: 195 DIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIVSTVNERIGSVAVVHTKDICR 254
           D +S+I + V GPF+T+++P S+   ++PITR+     I+     R G    VH  D+C 
Sbjct: 187 DFISIIPTLVVGPFITTSMPPSLITALSPITRNEAHYSII-----RQGQ--YVHLDDLCN 246

Query: 255 AHIFLMEHTKGKGRYLCCVGSYTLSELTE----RLSQHCDVKLERCVDEKQNWMRSEVGN 293
           AHIFL E    KGRY+C     T+  +++    +  ++        VDE  N    E  +
Sbjct: 247 AHIFLYEQAAAKGRYICSSHDATILTISKFLRPKYPEYNVPSTFEGVDE--NLKSIEFSS 306

BLAST of Cp4.1LG14g00070 vs. TAIR 10
Match: AT1G61720.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 146.4 bits (368), Expect = 3.7e-35
Identity = 99/325 (30.46%), Postives = 165/325 (50.77%), Query Frame = 0

Query: 17  CVTGATGFIGSSLVNLLLQNGYNVHATLRHPE---------------------------- 76
           CV G TG + S L+  LLQ+GY V+ T+R PE                            
Sbjct: 14  CVIGGTGNLASILIKHLLQSGYKVNTTVRDPENEKKIAHLRKLQELGDLKIFKADLTDED 73

Query: 77  ---------EYV--------------QTNVINPEIKGTINVLKSCMKSRSVKRVVLTSTI 136
                    EY+              + ++I P I+G INVLKSC+KS+SVKRV+ TS+ 
Sbjct: 74  SFESSFSGCEYIFHVATPINFKSEDPEKDMIKPAIQGVINVLKSCLKSKSVKRVIYTSSA 133

Query: 137 STLTGKDANGKRKRFVDESCQTFVDQVWKNKPSGWVYALSKRLSEDAAFKFAYENGIDIV 196
           + ++  + +G     ++E   T V+ + + KP  W Y +SK L+E  A++FA EN I++V
Sbjct: 134 AAVSINNLSG-TGIVMNEENWTDVEFLTEEKPFNWGYPISKVLAEKTAWEFAKENKINLV 193

Query: 197 SVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIVSTVNERIGSVAVVHTKDICRAHI 256
           +VI + ++G  L S+ PSS+ + M+ IT     +  +  + +  GS++ VH  D+ RAH+
Sbjct: 194 TVIPALIAGNSLLSDPPSSLSLSMSFITGKEMHVTGLKEMQKLSGSISFVHVDDLARAHL 253

Query: 257 FLMEHTKGKGRYLCCVGSYTLSELTERLSQ-HCDVKLERCVDEKQNWMRSEVGNKKLKDL 290
           FL E     GRY+CC  + ++ E+ + L Q +    +    +E  +  +  + ++KL + 
Sbjct: 254 FLAEKETASGRYICCAYNTSVPEIADFLIQRYPKYNVLSEFEEGLSIPKLTLSSQKLINE 313

BLAST of Cp4.1LG14g00070 vs. TAIR 10
Match: AT4G35420.1 (dihydroflavonol 4-reductase-like1 )

HSP 1 Score: 130.6 bits (327), Expect = 2.1e-30
Identity = 102/331 (30.82%), Postives = 155/331 (46.83%), Query Frame = 0

Query: 17  CVTGATGFIGSSLVNLLLQNGYNVHATLR------------------------------- 76
           CVTGA+GF+ S LV  LL  GY V  T+R                               
Sbjct: 9   CVTGASGFLASWLVKRLLLEGYEVIGTVRDPGNEKKLAHLWKLEGAKERLRLVKADLMEE 68

Query: 77  ---------------------HPEEYVQTNVINPEIKGTINVLKSCMKSRSVKRVVLTST 136
                                 P    +  ++ P I+GT+NVL+SC K+ S+KRVVLTS+
Sbjct: 69  GSFDNAIMGCQGVFHTASPVLKPTSNPEEEILRPAIEGTLNVLRSCRKNPSLKRVVLTSS 128

Query: 137 ISTLTGKDANGKRKRFVDESCQTFVD-----QVWKNKPSGWVYALSKRLSEDAAFKFAYE 196
            ST+  +D +   K  +DES  T V+     QVW        YALSK L+E AA+KF+ E
Sbjct: 129 SSTVRIRD-DFDPKIPLDESIWTSVELCKRFQVW--------YALSKTLAEQAAWKFSEE 188

Query: 197 NGIDIVSVITSTVSGPFLTSNVPSSVQVLMAPITRDPNFLRIVSTVNERIGSVAVVHTKD 256
           NGID+V+V+ S + GP L  ++ S+   ++         L+  +   +  G +  VH  D
Sbjct: 189 NGIDLVTVLPSFLVGPSLPPDLCSTASDVL-------GLLKGETEKFQWHGQMGYVHIDD 248

Query: 257 ICRAHIFLMEHTKGKGRYLCCVGSYTLSELTERLSQHCDVKLERCVDEKQNWMRSEVGNK 291
           + R HI + EH   +GRY+C     +L EL   LS            EK N +  +    
Sbjct: 249 VARTHIVVFEHEAAQGRYICSSNVISLEELVSFLSARYPSLPIPKRFEKLNRLHYDFDTS 308

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5XLY01.7e-4534.98Putative anthocyanidin reductase OS=Ginkgo biloba OX=3311 PE=2 SV=1[more]
Q5FB349.8e-4132.70Anthocyanidin reductase ((2S)-flavan-3-ol-forming) OS=Vitis vinifera OX=29760 GN... [more]
D7U6G69.8e-4132.39Anthocyanidin reductase ((2S)-flavan-3-ol-forming) OS=Vitis vinifera OX=29760 GN... [more]
Q7PCC42.8e-4032.39Anthocyanidin reductase ((2S)-flavan-3-ol-forming) OS=Vitis vinifera OX=29760 GN... [more]
P511021.0e-3730.24Dihydroflavonol 4-reductase OS=Arabidopsis thaliana OX=3702 GN=DFRA PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_023552763.17.97e-20183.91putative anthocyanidin reductase [Cucurbita pepo subsp. pepo][more]
XP_022985246.15.80e-18778.45putative anthocyanidin reductase [Cucurbita maxima][more]
KAG6577404.11.30e-18678.92hypothetical protein SDJN03_24978, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022929423.13.25e-18076.64putative anthocyanidin reductase [Cucurbita moschata][more]
XP_011650548.13.14e-14864.86putative anthocyanidin reductase [Cucumis sativus] >KAE8650161.1 hypothetical pr... [more]
Match NameE-valueIdentityDescription
A0A6J1J7L92.81e-18778.45putative anthocyanidin reductase OS=Cucurbita maxima OX=3661 GN=LOC111483297 PE=... [more]
A0A6J1ES231.57e-18076.64putative anthocyanidin reductase OS=Cucurbita moschata OX=3662 GN=LOC111435998 P... [more]
A0A6J1CP638.14e-14464.43putative anthocyanidin reductase OS=Momordica charantia OX=3673 GN=LOC111012896 ... [more]
A0A1S3BSY62.67e-14364.97dihydroflavonol-4-reductase isoform X8 OS=Cucumis melo OX=3656 GN=LOC103493381 P... [more]
A0A1S4DZ503.78e-14364.97dihydroflavonol-4-reductase isoform X7 OS=Cucumis melo OX=3656 GN=LOC103493381 P... [more]
Match NameE-valueIdentityDescription
AT4G27250.12.8e-7544.44NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G27250.21.9e-7143.59NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G42800.17.2e-3930.24dihydroflavonol 4-reductase [more]
AT1G61720.13.7e-3530.46NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G35420.12.1e-3030.82dihydroflavonol 4-reductase-like1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016040NAD(P)-binding domainPFAMPF16363GDP_Man_Dehydcoord: 18..51
e-value: 1.8E-6
score: 27.6
NoneNo IPR availableGENE3D3.40.50.720coord: 8..51
e-value: 5.2E-12
score: 47.5
NoneNo IPR availableGENE3D3.40.50.720coord: 52..290
e-value: 7.4E-59
score: 201.6
NoneNo IPR availablePANTHERPTHR10366:SF628NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEINcoord: 13..47
NoneNo IPR availablePANTHERPTHR10366:SF628NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEINcoord: 49..280
NoneNo IPR availablePANTHERPTHR10366NAD DEPENDENT EPIMERASE/DEHYDRATASEcoord: 49..280
NoneNo IPR availablePANTHERPTHR10366NAD DEPENDENT EPIMERASE/DEHYDRATASEcoord: 13..47
IPR001509NAD-dependent epimerase/dehydratasePFAMPF01370Epimerasecoord: 54..213
e-value: 9.1E-10
score: 38.4
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 13..286

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG14g00070.1Cp4.1LG14g00070.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
molecular_function GO:0003824 catalytic activity