Cp4.1LG13g10920 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG13g10920
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptiontRNA pseudouridine(38/39) synthase
LocationCp4.1LG13: 6576434 .. 6586101 (+)
RNA-Seq ExpressionCp4.1LG13g10920
SyntenyCp4.1LG13g10920
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGATGGTGTAAGCGATAGATGTAGCCATCGTACAGGCCAAGACAACGAACACAATCCGCCATCCAATTACTGCCGAGATCGCCATTGCAGCGCCTTGGACTTGAGCACTGTTGCTTTTATAGATGTGACAGTACTCACTTTTTAGACGGAGACGTTTGTGGCTCCCGGCTATTTTAATTTTGGATATTAATTATTTACTTATTTATATATTTTTTAATTTACAAATGTATTGAATTTATATTAAACATGTGATTCAATTTAATGTATTTAATATTGAAGTATGATTAATTTTGTCAATTAAAGTTACAATTAATAATAATAAACTATAATTAATATAAATTAATAAAGTATGAATAATTATTTTATACATAAATAATTTATATTGTTTTAGTAATAAATGGTATAAATGTGATTTATTCCACCCTATACCACATTGGATTCTTGAAACGGTTGGATGAAAGTCTCACGTCGGTTCAGGAATACTGTCTTCATTGGTGTGAGCTCTTTTGAGAAGCCCAAAGTAAAGCTACTGGAGCATCATACCACTGTGAGAGTCATGTTCATCTAACATGATAGTCATGCCCTAGATTTAGCAATGTCAATAGAATGAAGAAAGGACACTAAAAGAAAAAGATTCGAGCCTCGATTGAAGAGAGGTGTACTTTGTTCGAACCCAAATGTTGAATGAAAGTCCCACATCGGCTCGAGAGGCATACTTTGTTCGAGCCCATGTGTTGGATGAAAGTTTCACGTCCCCTACTTAAGCCCACGGAAGATACCGTACATGGTCTGCTACGCAAATTAGTACATCGGCGTGTATACTTGCTCTATAAATACGTTCCCGCCTTCTGACTTGGCTTTCTGAATAGACTATTTCGTCTCTTTCTTCTCGTCTTCAACCGCTGAATCTCAGTTTTCAGAGAACGGTCTCTGCTAGGGTTTTAGGGTTTCTTTTGTGATTTGATGCAACCTGGTGTTATATGATTTGCGGAATTTTTCCATTGCTGTAACATTGTGCTTCTTCTGTTCTTCAAGAAGCTTTCGTATGTGTTCTCTCGTCGTTTCTATGCGTCAATTCTCTTTTACCTCGCGATGAATTCCTACTTCTGTCTGAACTTTGGGGCCTTATAATTTGTTTTTCAGTGGTCGTTTGAACTCTGGGGCTGTATTTCTTGACGTACAAGGGCTAATGGACGGAGCGGAACATTCCCTCGATTACAGAGGAGGTACGGGAGCAATGGAAACGACCTCAGGAACTGACGAGTTTCAACCCTTCGATGGAAACCTAATCGGAAATCTTCAATCCCAGCTTCGTCTTCTCCAAGGCAGGGTTAAGGTTTGAATGTCTACTCTCATTTCAACTTCCGGTTGATTAATGACATAATGGAGAAGTATCGGTAGAGACAAGACTGATGTTCGATCTCAATCCACTAATCTGTCAGGAATTAGAGGAGGAAAATTCCAATCTATCCACTCGACTTGCAACTTGCTGCTGTTCTGAGGTTTGGTCGACTATGTTAGAATGAAATCAGAGCTATTTGGTTGTCATGCTAGCCCAGCTAACCTTAGGGTAGATTTTTCGTCAGGGCTAACTAAACATGAATTCGTGCAGAGGAGGATGAACTTTGACGACGGGAGTAGTTCGATTGAGAAAAGCAAGAAATATGGAAGTCTGAAAGACAAGACTACTAAGAAGAAAGCAGTAGAAAAAACGCCAGGCTAGTTGTTACTTTATGATCAAATAGAGTCTATTTTCTAATTCCTCCATAGTTGCGAAAATCAAATCAATTTTTTTCTTTTAGATAAGAGGATCACCAAAGAATGGTGCCATGGGGGAGTAGTAGTAGTAGTAACTCACATGTACATACGACCAAAGGAGGCAGAATGGAGAAGAATGTAATATGTAACAGTGGAGTTCATATAAATATATTAATGAGATTTTTATGAAATTCAAGAATCAAGATTAAGAAATGATGATTTGTGAGTCTGAACTATAGTCAAATTATGCATTGACATACAATAAAATACTCAAATGTGGCTGTTTCACAATAACTGTTGCATATGGATTTGAAAATTTGCATTTCCCCAATAGCCACTGGATAAACCAAGTAGTTTTTCCTACCTGCCAGACAAAAAGGTGAAAAATGATGAACCTTAGACATACGTTTGGAATGTTTATGAAGTTTAGATGAAATAATTTACGGTAGTTCTCATGCATATTATCAACCTTGTTTATCTACTGGAATGATTTGCTTGTTATTTTATGGGCGAAGGTTATAACACAAGGATCTTGGATCACTGTTCAAAGAGATATATTGCTCTAAAAGTAATGTATTTCGGGCGGAGGTATGTAATCTAGCGATAATGGGAACAAGACCAGTACCTTTTACTTTCCCCTGCATTAATTTCCGTACATTTCAGTCCTCCAAAACCTTCCTCCGAAATATGGAGAAATCTTGTTAAAAGAAAACCCCCCACAACAGTTGTTATTTAAAACTCTATCGTCGTAGCTTTTTGTTATACTGTGAAAATTTAACTTGATGTCAGATAGTGCCTGAAATTCTGGTTACTTCATCTGTTCAGGTTCTATGGCTTTGCTTCAGAGGCACAAATGGAACCAACAGTTGAGGTACAGTTTCAAATTGAACTTGAACAAATTATTTATGATTTATCAATTACAGTGATACTGATGGGGGATACTTGTCAAGAACGCACAATATTGATTCTTAATGTTTACTGGGAGTGCCTAAACATTTAAATAATGCATTTAGAGGATCCTAATATCAATTTTTTTAACCTTCCATCTTTTCTAATTGAGGTTTGATCACACTATAGCATCGTGCGATTCTCAAGAGTAAATATAAGGTTTTCTTTGTCAATGAGTATTCTTCCCTGCCTGCTTGCTAAGGAATGCCATCGAGATTAGTTTGATGCTGTGTTTAATTATGGATTTGGATTATAATAAAGAATGCTAGAAAGCACAATCAACTTTCTGATTCATCCGACCATTAGCTTTTTTATGAGTTTGAGAAATAAATGTCATGAATTTCTAAGTATGAATTATGGGGTAACCAGACTCCCTAGATTTCAGGAGGCTTGGATTTAACTAGAGTTTCACTATAGTAATGTGTAGCCCCTCCCTCCTTTACCCTTGCAAGTCCTCCTTACAGCCACCCTTTTTTCACCTTTCCCCCTCCTTTGTGATTCTCTTGCGATCCCAAAAGCAAACAAAAGGCTTAAGAGGTAAATGGAGCCAATAAAGGGAACTAAAGAGTAGGAATTGTCACTTGAAAAGACTTTTGACAAAGTTGATTGGCAACCTTTGAATGCTACATAAAAGTTGAGGGGCTTAGGAAATAGGTGAGCATGACTTCATGCGCAAGGCCATGGTCTGGGTGGGCGTAAACATTTAGCATCAATTTGTTTTAAGGGGGAGGGGGTTACGGGAAGGTAGATTTGGTGGTTGTGAGGAATGTTGGGTGAGAAGAGATTTACTTGTAAATTTTGTATCCAAAAAAAGAAGTTAATTTGTTATCAAATCTTCCTGCAGTCCGAAATTTTCAGAGCTCTTGAGAAAGCCAGGCTTCTTGTTGGTGGTAGGAAGGAGTCTTGTTATTCAAGATGTGGGAGAACAGACAGGGGGGTCTCATCTGTTGGGCAAGTAAGACATTTAAAATCTTTAGAGAATAACATTGTACAGCTAGAATAGCAACGATAAATTTATGCCAATTTTCTGAATACGTTGACTTCCTCATCACGAATTAGTTCACTTTTGGTTATTCTACTAGCTGTTCAAAATGACATTTACTGCCACCTCTTTCATGTCCTGGGGGTGTTTAGTTGAGGTGGGGAAATTGAAATCTAACAATACCTCTTTTATGTAGTTAACCCGAAATATCTTGTGATGTTCTTTACGTCTTTCTAATTTATATAAACACACAGATGACACACGAGAATCTAGCTCAAAAAGCTTTATTTTTTTAATATGAAAACCAAGGAGATGAAGATGTATAACATAATTCTTTTAGTTATTTGGTGTTTTCTACTATGGTTCTAATATTATTATGAAGGCTGCAGTTTTTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCTTTTACAAAAGCTTTTCTTGTTCTGTAAGTCATCAGTCACCTAGTACACAGAGAATCAGATTTTGAATTAGGGAACTTTGTTGTCCTTTCAACCATTATTTGAATTAATTTCCCCTTTCTAGCAATCACACTATTGTTGCTGCAGGTAATTGCTCTCTATTTACGGTCCAACCTTAAGGATCCCCCTAACGGTGAAAATGGCAATCATAATTCGGAAGAGCACCATGGTCAGCTAAGTTGATGTTGCCGAAGTTGTTCTCTGTTTTTAATAACTTGAATATTGAATCTAATCAGCTGAATGATATGGAGTGCATCAGACTTCTTGCTTTCTGTTACGGATAGCACTCTTGTTTGAAACATCAAAATATAGCAATATTCTTTACTGCATTAGGAAAGTAGATGGTAGCGATACTTTATACAGGGAACTGCAGACATGACCCTGGCTTTTATTTTTTGAAAGGATGTCATCTATAGTTTCATGTGCTTATTATTAGTTTTTAATAACATTTTCAATTGGGATAATTTTTTCTTTCTAAGTTCTAACTGGAACGATGCATACTTTTTCCATGTCTATTACCTCAACCTCACTTCCATTTATTATTTACTCTCAGATGGAGAAATTGATTATGTGAGAGTAATGAATAGAGTTCTTCCCAATGACATTCGGGTTTTAGGCTGGTGTACTGTTCCTGTTGGCTTCAGTGCAAGGTTTGTATTGCTCGTGATTTTATTTTTATAGATTTGGGTATTTTCCTTTTCTTGAGAGGAATATGATGTGTTAATTTTTAATGTGAAGTTAGGAACTTCATGAGACTAGCATTCAACAGATTTAAAAATCAATATAACTGGCTTTGACTGCTGAGTTTATGTGCCTACATGAAATTCTGCAGAGTTGTCTTGCTATTCTAAATGTCTGTACACCTCTCTAAGAAGTGCAGTGACCAGTAGCATCTCCATTACCCCTTACGTCATACAAGCAGCTTTACTTATGTGTTCTTTGTTAGATACAGCTGAATCTTTGTTAGATACAGCTGGATCTACATGCTGCTTAAGTTCTCTAGACATGCTTAACTTTGCCAGTGTATGGCATCATGGTCTGGGGATGACATTTTTTTTATTACGTTGGGGCATTGGTTTGAAACCAAGAACCATGTTAATTTTTTGGTTACTTTCTAAAAATTTAGTTTACACATCTTACATTTTTTCTAAAGATTAACATTTTTCTTCCAAGGGACTGGACCAATGGGGGTTATGGTTCATAATTATGTTAAAAATTAGTTTTGGAAAAGCCACATAGTAATTTATACCTAAACAATTCAAGAATATTCAAAATTTTAAGGTGTTATTGATAATCATGGGTACTTCATGATGCTCCCCATACACGAATACTCAATGCATGACCAATACTTCTTTGAGGAACTTATTATGTGAATTTGTTCCCGAAGTGTAAAACTTATCTATTCAACATAATATTTCTTTAAGGTATTAATCTGTTGGAAAGACTCCAGTTCTAGTTTCTAACATACATGCTTATATTATCGGAAAAATATTAATGCTTACTCTGGCATTTCTTGTTGCCTCTTTATGCAGCACAGATTTAGTTGTTTAAGAAGAAAGTATAAATATTTATTCTGGGGAGCAAACTTGAACATCTCGGTATGCAAATTATTTTGTATGAGTCTTGTAAAGCTTTTGCAGAAGTAATTTGGATGCTGCTTCAACAAAAGGTTGAAAAATTTTATCTGCATTTTCACCAGGCTATGGAGAGGGCCGGTAAGAAATTTGTAGGGGAACATGACTTCAGAAACTTCTGCAAAATGGATGCAGCAAATGTTCACAATTATAGGCGATGCATCATGTCATTTGACATTTCTCCTTGTGATGTGAGGTTGGCGTTGTTATCAGATTTCTAATCTGTTTTCCAATTCAACTTGTTGTTCCATGCTTAATTGAAACACGCCTTGCATTGTATATTTTTATTGTCAAATCTGATTAGCATATTTGCTTCTCAGGCCATCCAAAAGAGCCTTCCTCTAGCTGTTGTTCAAGCTTATGTTACTTTATTCTCCCTGTTTTTGGTCTCTTAAGTTCATTAACTATGTTCTCTCCTAGGTGTTGCCAAGCTGCCAGTTATTTACTAACTAGAATAAGAGTGCTTTTCTGACCATGTTCATTTTGAGTTGTTGATAATCATGTTGAAATTGACCATCTCTGTGGTTTTTATCATTTGCGGGGGTAGATTCATATTATGCAAGATGCTTTGAACTTCAGAAGAATGATCATGTTAACTTTTTAAGCGACCTGCAGTTATTGCTGTGTTAGATTTCTATTTATTCCAAATCTAGGATGCATTAAGACGACTACGCAAATTAAAAGAATGTGAAATATACTTGTGGGAGAAAGAAGCGTGGTACCATAGAACCCGTGTTTTATGATTGAATTCTATTGGATATATCTTTTGTAACTTCATTCTACATCATCGTGTCCTGAGCTAAGATGTCTTCTTGGCTCAAGACATCTACTTTTTTTTTTTTTGGTAAACAAATTAATCTCTCTCTCTCTCTCTCTCTCACAATCTTTTTAGTCTCTGAATCTTTTTGGTACTCTGACAGGATGGAAGGCAATCTATTATGGGCATTCAACATAGAAGGCAGTGCTTTCCTCTGGCATCAGGTCCGCTGCATGGTTGCTGTAGTATACATGGTTGGGCAAGGTCTTGAAGATCCTGATGTGCGTGCCAGACGTCTTGACATCTGATTGCTTTATTATATTTGCATAAACACTTTTAATATGTTAAACTGCTTGCTTTTGCTCATGAAAATGTAGTCATCTTATACTTCATAGGCCTCAACACTCTCATTTCAGCTTATTATATCTCTAGAATGGCCATAAAAGATAAACAACTAATGGTAAATTTGGTAACTAGGAGCTAGTGTTTTCCAGAATTGATAGCCGTATTTATTTTATATAGATGCATCTGCCGTTGACAATGAACCTTCCTAGCCACTGTTTCTTTTCAATACATGTGCACTTCCCCCCTTTACCGAAGTTATCAATCTTATTTCTTGGTTGGTAAACCCTAAGCATGACTTCGTTGCTCTTTTGAAATATGTGTTGTTTTGCTAATGGTCACACAATTAGCACTCATGTCAAGATTTCAACATTTAAAGTCGCTGTACACAGTGGTTTCCCAATTATCAGCTTTACTTGGCCTGTGGTGTGATTTTGTTATGCTATGTGAAGTATAAAATGCATCTCAATCTATTATATGAAGATGACTGGTAAAATTTGTAGGTGGTTGATATTCTACTGGATCCATTAAGAACACCAAGGAAGCCTCAGTATGTTATGGCTTCAGAAAATCCTCTGGTTCTTCATTCTTGTGAATTTGAAGGTGTCAAATTTAGGTGTTCAACAGGTACTTTTCTTCAGCTTCATTAAGGGATTAATTGATTATAGATAACGTGACTTCTACCTCCTGTATATTTTCTTTTTTCACCTCAGTTGTCATTATACTATTGTTCTATATTTAAAGGATTTCCATGCCTGCATTTCTCCTAGATGCTGGAGAAGCTCTACAGTTGCATTTAACAAATGAAATCCGCACATATGAGCTCCAAGCTGCAATATTCAAAGAAGCTCTTCTAAGCTGTTTGCCAATCACAAATGGTTTGAATTGCAATCTTTTCCTATTATACATTTTTGTCATTATTGTCGGTTGATTATGATACTGATGTGGCAACTTCCATCTCTCCAGATGAGAGCTTTCCAGACAACAGAAAGATAAAGAAGAAAATTTGCCATGTTCCCCTATTTTCAAGACCAACTGAACGTAAGTTCTGAACAAAAAGACTGCTGACTATTTTGGGATTATTTATTTGATAATGTCATATAGTACTACACTATCTGTGATTCTGTATCTAACCAATGACTAAACAACTGAATATATTATTTTAGTATCAAAGTTAAAAAGAAAAACACTTCAGCTAAACGCAAATCCAATGACCCTTGTGTACATGTCAGTTAATAATTTGGTGTGAGCTGTTTTGTCCATATCCATTTACATAATGTTTTTAGTTAATGTGGAGCTTTTGTTTTGTCCATAGCAGAATCACCTCTCTTTCTTTGCTAATGATTTATGGGCCAGTAAGTCTAGCTGTCATGGAGGAAGATGTTCATCGAGAAATGTGAGATCCCACATCGGTTGAAGAGGGGAACGAAGCATTCCTTATAAGGGTGTGGAAACCTCTCCCTAGTAGACGCATTATAAAACCTTGAGGGGAAGTCTTTGAAAGAAAGCTCAAAGAAAACAATATTTGCTAGCGGCGGGCTTGGTCTGTTACAAGAAATTAAAATATTAGCTACATGATTGGTTTTCATTTAATTTATTTGATTTATTGAATCCCTCTATCTTAAGCGTTGCCTTTTCCCCTTCTCATCCCCAAACTATATTATTAACTACCTCCAAAAGCATACCTTTCCCTCTGTTATTTCAATATGTTAGTTTATATGTATTGGTTTATGCTGTTCCGTTTCCAGCTTCTTATGAAGAGCGGCGTGCCAAGCTCAACTCATCCTCAAAGAGTTCATCAAATACATCTTCATAGGCACAGGTAACATAGTTTTCATGTTTTTTGAAAAATAAAAAATAAATCTCTGAGCATCTGTTTCTAAGATCTAATTTCTTCCCTTTCAAAAAGTTATCAAGTGAACATGAAAGCCTAATTAGACTAATTATGATACTATATGGATACAAGATTATATCTTAGAGCAGCTCTCATCCCATTTCATAAGAGACTGAAGTCTTTTAAAACTGCATTTGGCCTAACTTTTAGAATACTAATTAAGGAACTTCTCAAATCTACTGTTCAATGTTCTTTATATTTATGTTTATTTCTTATTATAACTCAAAGAAACATTTGAATTAGTGTACAATAAGGGGGTAAATTTAGAGATAATGAGTGTATCTGTAGTTTTGCAGAGTTTGAACGCCATTAAAAGAGCAGAAGTGGCAATGATGAGCATCCGGTGATGAAGGGGAGATGAGTCTAAAGAAAAAGCTGACAGCCCAACCACACCCCAACATCAACATGTACCATCATCATCATGAATCCAGGTGCATGAACTCCGACCAAATCATTTTTCTCACTTGCCCTGTCCCCCTCTCCTCTATGGTTTTATTAATGTTGAGAATGACTTGTGACGGTTCTGTGCTGTGTGTTTTTGTCCTGAACCATGTGAAAGCTTAAACCATTTTAGAGGAAGCTATGTTTTTTTAGTTAAAATCTAGGTTAAATTATATAAATTACCCCGAACTTCTGTATCAGGCCTCAATTATATACCTATAGGCTTCGAAAAGTTTCATTTTTACTCTTGAACTTTGAAATTTGACCCAAAGAATAATACTCTTAGAGCGGAAAATGTTTCTTGTCTTTTTAAACACTATTCTCGAAGTGTTAGGGTAAAGTTGAAAACTTTAGAAGTCGTAGTGTCTTAAAATTTAACCATGATCAACTATGGTGAAAATATAAATTTCTTATCGATTTA

mRNA sequence

ATGGCGATGACTATTTCGTCTCTTTCTTCTCGTCTTCAACCGCTGAATCTCAGTTTTCAGAGAACGGTCTCTGCTAGGGTTTTAGGTGGTCGTTTGAACTCTGGGGCTGTATTTCTTGACGTACAAGGGCTAATGGACGGAGCGGAACATTCCCTCGATTACAGAGGAGGTACGGGAGCAATGGAAACGACCTCAGGAACTGACGAGTTTCAACCCTTCGATGGAAACCTAATCGGAAATCTTCAATCCCAGCTTCGTCTTCTCCAAGGCAGGGTTAAGGAATTAGAGGAGGAAAATTCCAATCTATCCACTCGACTTGCAACTTGCTGCTGTTCTGAGAGGAGGATGAACTTTGACGACGGGAGTAGTTCGATTGAGAAAAGCAAGAAATATGGAAGTCTGAAAGACAAGACTACTAAGAAGAAAGCAATAAGAGGATCACCAAAGAATGGTTATAACACAAGGATCTTGGATCACTGTTCAAAGAGATATATTGCTCTAAAAGTAATGTATTTCGGGCGGAGGTTCTATGGCTTTGCTTCAGAGGCACAAATGGAACCAACAGTTGAGTCCGAAATTTTCAGAGCTCTTGAGAAAGCCAGGCTTCTTGTTGGTGGTAGGAAGGAGTCTTGTTATTCAAGATGTGGGAGAACAGACAGGGGGGTCTCATCTGTTGGGCAAGTAATTGCTCTCTATTTACGGTCCAACCTTAAGGATCCCCCTAACGGTGAAAATGGCAATCATAATTCGGAAGAGCACCATGATGGAGAAATTGATTATGTGAGAGTAATGAATAGAGTTCTTCCCAATGACATTCGGGTTTTAGGCTGGTGTACTGTTCCTGTTGGCTTCAGTGCAAGATTTAGTTGTTTAAGAAGAAAGTATAAATATTTATTCTGGGGAGCAAACTTGAACATCTCGGCTATGGAGAGGGCCGGTAAGAAATTTGTAGGGGAACATGACTTCAGAAACTTCTGCAAAATGGATGCAGCAAATGTTCACAATTATAGGCGATGCATCATGTCATTTGACATTTCTCCTTGTGATGTGAGGATGGAAGGCAATCTATTATGGGCATTCAACATAGAAGGCAGTGCTTTCCTCTGGCATCAGGTCCGCTGCATGGTTGCTGTAGTATACATGGTTGGGCAAGGTCTTGAAGATCCTGATGTGGTTGATATTCTACTGGATCCATTAAGAACACCAAGGAAGCCTCAGTATGTTATGGCTTCAGAAAATCCTCTGGTTCTTCATTCTTGTGAATTTGAAGGTGTCAAATTTAGGATTTCCATGCCTGCATTTCTCCTAGATGCTGGAGAAGCTCTACAGTTGCATTTAACAAATGAAATCCGCACATATGAGCTCCAAGCTGCAATATTCAAAGAAGCTCTTCTAAGCTGTTTGCCAATCACAAATGATGAGAGCTTTCCAGACAACAGAAAGATAAAGAAGAAAATTTGCCATGTTCCCCTATTTTCAAGACCAACTGAACTTTTGCAGAGTTTGAACGCCATTAAAAGAGCAGAAGTGGCAATGATGAGCATCCGGTGATGAAGGGGAGATGAGTCTAAAGAAAAAGCTGACAGCCCAACCACACCCCAACATCAACATGTACCATCATCATCATGAATCCAGGTGCATGAACTCCGACCAAATCATTTTTCTCACTTGCCCTGTCCCCCTCTCCTCTATGGTTTTATTAATGTTGAGAATGACTTGTGACGGTTCTGTGCTGTGTGTTTTTGTCCTGAACCATGTGAAAGCTTAAACCATTTTAGAGGAAGCTATGTTTTTTTAGTTAAAATCTAGGTTAAATTATATAAATTACCCCGAACTTCTGTATCAGGCCTCAATTATATACCTATAGGCTTCGAAAAGTTTCATTTTTACTCTTGAACTTTGAAATTTGACCCAAAGAATAATACTCTTAGAGCGGAAAATGTTTCTTGTCTTTTTAAACACTATTCTCGAAGTGTTAGGGTAAAGTTGAAAACTTTAGAAGTCGTAGTGTCTTAAAATTTAACCATGATCAACTATGGTGAAAATATAAATTTCTTATCGATTTA

Coding sequence (CDS)

ATGGCGATGACTATTTCGTCTCTTTCTTCTCGTCTTCAACCGCTGAATCTCAGTTTTCAGAGAACGGTCTCTGCTAGGGTTTTAGGTGGTCGTTTGAACTCTGGGGCTGTATTTCTTGACGTACAAGGGCTAATGGACGGAGCGGAACATTCCCTCGATTACAGAGGAGGTACGGGAGCAATGGAAACGACCTCAGGAACTGACGAGTTTCAACCCTTCGATGGAAACCTAATCGGAAATCTTCAATCCCAGCTTCGTCTTCTCCAAGGCAGGGTTAAGGAATTAGAGGAGGAAAATTCCAATCTATCCACTCGACTTGCAACTTGCTGCTGTTCTGAGAGGAGGATGAACTTTGACGACGGGAGTAGTTCGATTGAGAAAAGCAAGAAATATGGAAGTCTGAAAGACAAGACTACTAAGAAGAAAGCAATAAGAGGATCACCAAAGAATGGTTATAACACAAGGATCTTGGATCACTGTTCAAAGAGATATATTGCTCTAAAAGTAATGTATTTCGGGCGGAGGTTCTATGGCTTTGCTTCAGAGGCACAAATGGAACCAACAGTTGAGTCCGAAATTTTCAGAGCTCTTGAGAAAGCCAGGCTTCTTGTTGGTGGTAGGAAGGAGTCTTGTTATTCAAGATGTGGGAGAACAGACAGGGGGGTCTCATCTGTTGGGCAAGTAATTGCTCTCTATTTACGGTCCAACCTTAAGGATCCCCCTAACGGTGAAAATGGCAATCATAATTCGGAAGAGCACCATGATGGAGAAATTGATTATGTGAGAGTAATGAATAGAGTTCTTCCCAATGACATTCGGGTTTTAGGCTGGTGTACTGTTCCTGTTGGCTTCAGTGCAAGATTTAGTTGTTTAAGAAGAAAGTATAAATATTTATTCTGGGGAGCAAACTTGAACATCTCGGCTATGGAGAGGGCCGGTAAGAAATTTGTAGGGGAACATGACTTCAGAAACTTCTGCAAAATGGATGCAGCAAATGTTCACAATTATAGGCGATGCATCATGTCATTTGACATTTCTCCTTGTGATGTGAGGATGGAAGGCAATCTATTATGGGCATTCAACATAGAAGGCAGTGCTTTCCTCTGGCATCAGGTCCGCTGCATGGTTGCTGTAGTATACATGGTTGGGCAAGGTCTTGAAGATCCTGATGTGGTTGATATTCTACTGGATCCATTAAGAACACCAAGGAAGCCTCAGTATGTTATGGCTTCAGAAAATCCTCTGGTTCTTCATTCTTGTGAATTTGAAGGTGTCAAATTTAGGATTTCCATGCCTGCATTTCTCCTAGATGCTGGAGAAGCTCTACAGTTGCATTTAACAAATGAAATCCGCACATATGAGCTCCAAGCTGCAATATTCAAAGAAGCTCTTCTAAGCTGTTTGCCAATCACAAATGATGAGAGCTTTCCAGACAACAGAAAGATAAAGAAGAAAATTTGCCATGTTCCCCTATTTTCAAGACCAACTGAACTTTTGCAGAGTTTGAACGCCATTAAAAGAGCAGAAGTGGCAATGATGAGCATCCGGTGA

Protein sequence

MAMTISSLSSRLQPLNLSFQRTVSARVLGGRLNSGAVFLDVQGLMDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLSTRLATCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAIRGSPKNGYNTRILDHCSKRYIALKVMYFGRRFYGFASEAQMEPTVESEIFRALEKARLLVGGRKESCYSRCGRTDRGVSSVGQVIALYLRSNLKDPPNGENGNHNSEEHHDGEIDYVRVMNRVLPNDIRVLGWCTVPVGFSARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVYMVGQGLEDPDVVDILLDPLRTPRKPQYVMASENPLVLHSCEFEGVKFRISMPAFLLDAGEALQLHLTNEIRTYELQAAIFKEALLSCLPITNDESFPDNRKIKKKICHVPLFSRPTELLQSLNAIKRAEVAMMSIR
Homology
BLAST of Cp4.1LG13g10920 vs. ExPASy Swiss-Prot
Match: Q9JI38 (tRNA pseudouridine(38/39) synthase OS=Mus musculus OX=10090 GN=Pus3 PE=1 SV=2)

HSP 1 Score: 258.8 bits (660), Expect = 1.3e-67
Identity = 156/416 (37.50%), Postives = 233/416 (56.01%), Query Frame = 0

Query: 82  QSQLRLLQGRVKELEEENSNLSTRLATCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKK 141
           ++Q+  L  RVKELE+E   L                       +K ++  ++KD + ++
Sbjct: 7   RNQIEKLLNRVKELEQEVERLK----------------------KKKEQANNIKDSSIRE 66

Query: 142 KAI-RGSPKNGYNTRILDHCSKRYIALKVMYFGRRFYGFASEAQMEPTVESEIFRALEKA 201
            ++  G  K  ++        +R++ALK+ Y G  + GFAS+     T+E ++F AL K 
Sbjct: 67  NSLGSGKAKRAFD---FSAHGRRHVALKIAYLGWGYQGFASQENTSNTIEEKLFEALTKT 126

Query: 202 RLLVGGRKESCYSRCGRTDRGVSSVGQVIALYLRSNLKDPPNGENGNHNSE-EHHDGEID 261
           R LV  R+ S Y RCGRTD+GVS+ GQVI+L LRS      + E+ N   E +    EI 
Sbjct: 127 R-LVESRQTSNYHRCGRTDKGVSAFGQVISLDLRSQFPTSRDSEDSNLKHEADDLAKEIR 186

Query: 262 YVRVMNRVLPNDIRVLGWCTVPVGFSARFSCLRRKYKYLFWGANLNISAMERAGKKFVGE 321
           Y  ++NRVLP DIRVL W  V   FSARFSCL R Y+Y F  A+L+I+ M  A +K+VG 
Sbjct: 187 YTHILNRVLPADIRVLAWAPVEPSFSARFSCLERTYRYFFPRADLDIATMNYAAQKYVGT 246

Query: 322 HDFRNFCKMDAAN-VHNYRRCIMSFDI-----SPCDV-RMEGNLLWAFNIEGSAFLWHQV 381
           HDFRN CKMD AN V N++R I+   +     SP +  R E   L  F + G AFL+HQV
Sbjct: 247 HDFRNLCKMDVANGVINFQRTILCAQVQLVAQSPGEERRQEPFQLCQFEVIGQAFLYHQV 306

Query: 382 RCMVAVVYMVGQGLEDPDVVDILLDPLRTPRKPQYVMASENPLVLHSCEFEGVKFRISMP 441
           RCM+A+++++GQG+E P+++D LL+  + P+KPQY MA E PLVL+ C+FE  K+     
Sbjct: 307 RCMMAILFLIGQGMEKPEIIDELLNIQKNPQKPQYSMAVEFPLVLYDCKFENTKWIYDHE 366

Query: 442 AFLLDAGEALQLHLTNEIRTYELQAAI--FKEALLSCLPITNDESFPDNRKIKKKI 487
               +     QL   + ++T+ L + +      +++C   T  +   + R I+  +
Sbjct: 367 VQEFNVTHLQQLWANHAVKTHMLYSMLQGLDSVMVTCAAGTKMDEATEWRNIQPPV 396

BLAST of Cp4.1LG13g10920 vs. ExPASy Swiss-Prot
Match: Q3SX07 (tRNA pseudouridine(38/39) synthase OS=Bos taurus OX=9913 GN=PUS3 PE=2 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.8e-66
Identity = 144/341 (42.23%), Postives = 204/341 (59.82%), Query Frame = 0

Query: 129 KKYGSLKDKTTKKKA--IRGSPKNGYNTRILDHCSKRYIALKVMYFGRRFYGFASEAQME 188
           K+  + KD   ++ +    G PK  ++        +R++ALK+ Y G  + GFAS+    
Sbjct: 30  KEQANNKDSNIRENSSGAGGKPKRAFD---FSAHGQRHVALKIAYLGWGYQGFASQENTS 89

Query: 189 PTVESEIFRALEKARLLVGGRKESCYSRCGRTDRGVSSVGQVIALYLRSNLKDPPNGENG 248
            T+E ++F AL K R LV  R+ S Y RCGRTD+GVS+ GQVI+L LRS++    + E+ 
Sbjct: 90  NTIEEKLFEALTKTR-LVENRQTSNYHRCGRTDKGVSAFGQVISLDLRSHIPKGRDSEHF 149

Query: 249 NHNSEEHHDG-EIDYVRVMNRVLPNDIRVLGWCTVPVGFSARFSCLRRKYKYLFWGANLN 308
           N  +E +    EI Y  ++NRVLP DIRVL W  V   FSARFSCL R Y+Y F  ANL+
Sbjct: 150 NLKNEVNDVATEIRYTHILNRVLPPDIRVLAWAPVETSFSARFSCLERTYRYFFPRANLD 209

Query: 309 ISAMERAGKKFVGEHDFRNFCKMDAAN-VHNYRRCIMSFDISPCDVRM------EGNLLW 368
           I  M  A +K+VG HDFRN CKMD AN V N++R I+S  +      +      E   L 
Sbjct: 210 IVTMNYAAQKYVGTHDFRNLCKMDVANGVINFQRTILSAQVQRVGQNLGEEGWQEPFQLC 269

Query: 369 AFNIEGSAFLWHQVRCMVAVVYMVGQGLEDPDVVDILLDPLRTPRKPQYVMASENPLVLH 428
            F + G AFL+HQVRCM+AV++++GQG+E P+V+D LL+  + P+KPQY MA E PLVL+
Sbjct: 270 QFEVTGQAFLYHQVRCMMAVLFLIGQGMEKPEVIDELLNIEKNPQKPQYSMAVEFPLVLY 329

Query: 429 SCEFEGVKFRISMPAFLLDAGEALQLHLTNEIRTYELQAAI 460
            C+FE +K+         +     QL   + ++T  L + +
Sbjct: 330 DCKFENIKWIYDREVQEFNVTHLQQLWANHAVKTQMLYSML 366

BLAST of Cp4.1LG13g10920 vs. ExPASy Swiss-Prot
Match: Q9BZE2 (tRNA pseudouridine(38/39) synthase OS=Homo sapiens OX=9606 GN=PUS3 PE=1 SV=3)

HSP 1 Score: 254.6 bits (649), Expect = 2.4e-66
Identity = 153/386 (39.64%), Postives = 222/386 (57.51%), Query Frame = 0

Query: 82  QSQLRLLQGRVKELEEENSNLSTRLATCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKK 141
           ++Q   L  RV+ELE+E   L    A                   K+K+  ++++ +   
Sbjct: 8   RNQTEKLLKRVRELEQEVQRLKKEQA-------------------KNKEDSNIRENS--- 67

Query: 142 KAIRGSPKNGYNTRILDHCSKRYIALKVMYFGRRFYGFASEAQMEPTVESEIFRALEKAR 201
            A  G  K  ++        +R++AL++ Y G  + GFAS+     T+E ++F AL K R
Sbjct: 68  -AGAGKTKRAFD---FSAHGRRHVALRIAYMGWGYQGFASQENTNNTIEEKLFEALTKTR 127

Query: 202 LLVGGRKESCYSRCGRTDRGVSSVGQVIALYLRSNLKDPPNGENGNHNSEEHHDG-EIDY 261
            LV  R+ S Y RCGRTD+GVS+ GQVI+L LRS      + E+ N   E +    EI Y
Sbjct: 128 -LVESRQTSNYHRCGRTDKGVSAFGQVISLDLRSQFPRGRDSEDFNVKEEANAAAEEIRY 187

Query: 262 VRVMNRVLPNDIRVLGWCTVPVGFSARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEH 321
             ++NRVLP DIR+L W  V   FSARFSCL R Y+Y F  A+L+I  M+ A +K+VG H
Sbjct: 188 THILNRVLPPDIRILAWAPVEPSFSARFSCLERTYRYFFPRADLDIVTMDYAAQKYVGTH 247

Query: 322 DFRNFCKMDAAN-VHNYRRCIMSFDI-----SPCDVR-MEGNLLWAFNIEGSAFLWHQVR 381
           DFRN CKMD AN V N++R I+S  +     SP + R  E   L  F + G AFL+HQVR
Sbjct: 248 DFRNLCKMDVANGVINFQRTILSAQVQLVGQSPGEGRWQEPFQLCQFEVTGQAFLYHQVR 307

Query: 382 CMVAVVYMVGQGLEDPDVVDILLDPLRTPRKPQYVMASENPLVLHSCEFEGVKFRISMPA 441
           CM+A+++++GQG+E P+++D LL+  + P+KPQY MA E PLVL+ C+FE VK+     A
Sbjct: 308 CMMAILFLIGQGMEKPEIIDELLNIEKNPQKPQYSMAVEFPLVLYDCKFENVKWIYDQEA 366

Query: 442 FLLDAGEALQLHLTNEIRTYELQAAI 460
              +     QL   + ++T+ L + +
Sbjct: 368 QEFNITHLQQLWANHAVKTHMLYSML 366

BLAST of Cp4.1LG13g10920 vs. ExPASy Swiss-Prot
Match: P31115 (tRNA pseudouridine(38/39) synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DEG1 PE=1 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 9.7e-60
Identity = 143/362 (39.50%), Postives = 212/362 (58.56%), Query Frame = 0

Query: 125 IEKSKKYGSLKDKTTKKKAIRGSPKNGYNTRILDHCSKRYIALKVMYFGRRFYGFASEAQ 184
           IE +KK    +++ T+ KA +   K  ++       + R+IAL+  Y G  + G A + +
Sbjct: 59  IEDNKKRKISQEEVTRSKAKKAPKKFDFSKH-----NTRFIALRFAYLGWNYNGLAVQKE 118

Query: 185 ME--PTVESEIFRALEKARLLVG-GRKESCYSRCGRTDRGVSSVGQVIALYLRSNLKDPP 244
               PTVE  I  A+ K +L+     ++  +SRCGRTD+GVS++ QVI+L +RSNL D  
Sbjct: 119 YTPLPTVEGTILEAMNKCKLVPSMVLQDYKFSRCGRTDKGVSAMNQVISLEVRSNLTDEE 178

Query: 245 NGENGNHNSEEHHDGEIDYVRVMNRVLPNDIRVLGWC-TVPVGFSARFSCLRRKYKYLFW 304
             +  N +       EI YV V+N++LP+DIR+   C   P  F ARFSC+ R YKY+F 
Sbjct: 179 QRDPTNDSR------EIPYVHVLNQLLPDDIRISAVCLRPPPNFDARFSCVHRHYKYIFN 238

Query: 305 GANLNISAMERAGKKFVGEHDFRNFCKMDAA-NVHNYRRCIMSFDISPCDVRMEGNLLWA 364
           G NLNI  M +A   FVGE DFRNFCK+D +  + N++R I+S  I P          + 
Sbjct: 239 GKNLNIEKMSKAASYFVGERDFRNFCKLDGSKQITNFKRTIISSKILPL-----SETFYC 298

Query: 365 FNIEGSAFLWHQVRCMVAVVYMVGQGLEDPDVVDILLDPLRTPRKPQYVMASENPLVLHS 424
           F++ GSAFLWHQVRCM+A++++VGQ LE P++V  L D  +TP++P Y MA++ PL+L+ 
Sbjct: 299 FDLVGSAFLWHQVRCMMAILFLVGQSLEVPEIVLRLTDIEKTPQRPVYEMANDIPLLLYD 358

Query: 425 CEFEGVKFRISMPAFLLDAGEALQLHLTNEIRT--YELQAA---IFKEALLSCLPITNDE 477
           C+F  + ++       +D  +A++     E  T  YEL+AA   IFK+     LP  N  
Sbjct: 359 CKFPEMDWQEP----TVDDYKAIKFTTATEALTLHYELKAAVCNIFKDV----LPTANTN 396

BLAST of Cp4.1LG13g10920 vs. ExPASy Swiss-Prot
Match: Q9UTB3 (Putative tRNA pseudouridine synthase C25B8.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC25B8.05 PE=3 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.9e-55
Identity = 117/274 (42.70%), Postives = 171/274 (62.41%), Query Frame = 0

Query: 164 YIALKVMYFGRRFYGFASEAQME--PTVESEIFRALEKARLLVGGRKESC-YSRCGRTDR 223
           ++A K  Y G  + G A + +    PTVE ++F AL +A L+      SC +SRCGRTD+
Sbjct: 93  FVAFKFAYCGWNYNGLAYQLEPTPLPTVEGKVFEALLRAHLITD--PSSCSFSRCGRTDK 152

Query: 224 GVSSVGQVIALYLRSNLKDPPNGENGNHNSEEHHDGEIDYVRVMNRVLPNDIRVLGWCTV 283
           GVS++GQVI+L +R + + P                 I Y  V+NR+LP DIR+ G+   
Sbjct: 153 GVSAMGQVISLNVRHSERRP-----------------IIYCDVLNRLLPADIRITGYAFP 212

Query: 284 PVGFSARFSCLRRKYKYLFW----GANLNISAMERAGKKFVGEHDFRNFCKMDAA-NVHN 343
           P GF ARFSC +R YKYLF     G +L+I  M  A   ++GEHDFRNFCK+DA+  + N
Sbjct: 213 PEGFDARFSCKQRHYKYLFTKDYPGGSLDIDRMNEAAALYLGEHDFRNFCKIDASKQITN 272

Query: 344 YRRCIMSFDISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVYMVGQGLEDPDVVDIL 403
           Y R I+S  +   D       L+AF+++G+AFLWHQVRCM+A+++++GQ LE   +++ L
Sbjct: 273 YHRRILSSKVICID---PSTGLYAFDLQGTAFLWHQVRCMMAILFLIGQKLEPASLINDL 332

Query: 404 LDPLRTPRKPQYVMASENPLVLHSCEFEGVKFRI 430
           LD  + P KP Y MASE PL+L+ C+F+ +++ +
Sbjct: 333 LDIKKVPTKPIYDMASEYPLILYDCDFDNIEWTL 344

BLAST of Cp4.1LG13g10920 vs. NCBI nr
Match: KAG6578858.1 (putative F-actin-capping protein subunit beta, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 904 bits (2337), Expect = 0.0
Identity = 449/470 (95.53%), Postives = 455/470 (96.81%), Query Frame = 0

Query: 28  LGGRLNSGAVFLDVQGLMDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRL 87
           L GRLNSGAVFLDVQGLMDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRL
Sbjct: 3   LSGRLNSGAVFLDVQGLMDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRL 62

Query: 88  LQGRVKELEEENSNLSTRLATCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAIRGS 147
           LQGRVKELEEENSNLS+RLA+CCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKA+  +
Sbjct: 63  LQGRVKELEEENSNLSSRLASCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAVEKT 122

Query: 148 PKNGYNTRILDHCSKRYIALKVMYFGRRFYGFASEAQMEPTVESEIFRALEKARLLVGGR 207
           P  GYNTRILDHCSKRYIALKVMYFGRRFYGFASEAQMEPTVESEIFRALEKARLLVGGR
Sbjct: 123 P--GYNTRILDHCSKRYIALKVMYFGRRFYGFASEAQMEPTVESEIFRALEKARLLVGGR 182

Query: 208 KESCYSRCGRTDRGVSSVGQVIALYLRSNLKDPPNGENGNHNSEEHHDGEIDYVRVMNRV 267
           KESCYSRCGRTDRGVSSVGQVIALYLRSNLKDPPNGENGNHNSEEHHDGEIDYVRVMNRV
Sbjct: 183 KESCYSRCGRTDRGVSSVGQVIALYLRSNLKDPPNGENGNHNSEEHHDGEIDYVRVMNRV 242

Query: 268 LPNDIRVLGWCTVPVGFSARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCK 327
           LPNDIRVLGWCTVPVGFSARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCK
Sbjct: 243 LPNDIRVLGWCTVPVGFSARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCK 302

Query: 328 MDAANVHNYRRCIMSFDISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVYMVGQGLE 387
           MDAANVHNYRRCIMSFDISPCDVRMEGN LWAFNIEGSAFLWHQVRCMVAVV+MVGQGLE
Sbjct: 303 MDAANVHNYRRCIMSFDISPCDVRMEGNPLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLE 362

Query: 388 DPDVVDILLDPLRTPRKPQYVMASENPLVLHSCEFEGVKFRISMPAFLLDAGEALQLHLT 447
           DPDVVDILLDP+RTPRKPQYVMASENPLVLHSCEFEGVKFR S      DAGEALQLHLT
Sbjct: 363 DPDVVDILLDPVRTPRKPQYVMASENPLVLHSCEFEGVKFRCST-----DAGEALQLHLT 422

Query: 448 NEIRTYELQAAIFKEALLSCLPITNDESFPDNRKIKKKICHVPLFSRPTE 497
           NEIRTYELQAAIFKEALLSCLPITNDESF DNRKIKKKICHVPL SRPTE
Sbjct: 423 NEIRTYELQAAIFKEALLSCLPITNDESFSDNRKIKKKICHVPLLSRPTE 465

BLAST of Cp4.1LG13g10920 vs. NCBI nr
Match: XP_023551060.1 (tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita pepo subsp. pepo] >XP_023551061.1 tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 889 bits (2296), Expect = 0.0
Identity = 440/453 (97.13%), Postives = 442/453 (97.57%), Query Frame = 0

Query: 45  MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLST 104
           MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLST
Sbjct: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLST 60

Query: 105 RLATCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAIRGSPKNGYNTRILDHCSKRY 164
           RLATCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKA+  +P  GYNTRILDHCSKRY
Sbjct: 61  RLATCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAVEKTP--GYNTRILDHCSKRY 120

Query: 165 IALKVMYFGRRFYGFASEAQMEPTVESEIFRALEKARLLVGGRKESCYSRCGRTDRGVSS 224
           IALKVMYFGRRFYGFASEAQMEPTVESEIFRALEKARLLVGGRKESCYSRCGRTDRGVSS
Sbjct: 121 IALKVMYFGRRFYGFASEAQMEPTVESEIFRALEKARLLVGGRKESCYSRCGRTDRGVSS 180

Query: 225 VGQVIALYLRSNLKDPPNGENGNHNSEEHHDGEIDYVRVMNRVLPNDIRVLGWCTVPVGF 284
           VGQVIALYLRSNLKDPPNGENGNHNSEEHHDGEIDYVRVMNRVLPNDIRVLGWCTVPVGF
Sbjct: 181 VGQVIALYLRSNLKDPPNGENGNHNSEEHHDGEIDYVRVMNRVLPNDIRVLGWCTVPVGF 240

Query: 285 SARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD 344
           SARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD
Sbjct: 241 SARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD 300

Query: 345 ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVYMVGQGLEDPDVVDILLDPLRTPRK 404
           ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVYMVGQGLEDPDVVDILLDPLRTPRK
Sbjct: 301 ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVYMVGQGLEDPDVVDILLDPLRTPRK 360

Query: 405 PQYVMASENPLVLHSCEFEGVKFRISMPAFLLDAGEALQLHLTNEIRTYELQAAIFKEAL 464
           PQYVMASENPLVLHSCEFEGVKFR S      DAGEALQLHLTNEIRTYELQAAIFKEAL
Sbjct: 361 PQYVMASENPLVLHSCEFEGVKFRCST-----DAGEALQLHLTNEIRTYELQAAIFKEAL 420

Query: 465 LSCLPITNDESFPDNRKIKKKICHVPLFSRPTE 497
           LSCLPITNDESFPDNRKIKKKICHVPLFSRPTE
Sbjct: 421 LSCLPITNDESFPDNRKIKKKICHVPLFSRPTE 446

BLAST of Cp4.1LG13g10920 vs. NCBI nr
Match: XP_022938732.1 (tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita moschata] >XP_022938734.1 tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita moschata])

HSP 1 Score: 865 bits (2235), Expect = 9.85e-315
Identity = 427/453 (94.26%), Postives = 438/453 (96.69%), Query Frame = 0

Query: 45  MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLST 104
           MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQS+LRLLQGRVK LEEENSNLS+
Sbjct: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSRLRLLQGRVKVLEEENSNLSS 60

Query: 105 RLATCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAIRGSPKNGYNTRILDHCSKRY 164
           RLATCCCSERRMNFDDGSSSIEKS+KYGSLKDKTTKKKA+  +P  GYNTRILDHCSKRY
Sbjct: 61  RLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTP--GYNTRILDHCSKRY 120

Query: 165 IALKVMYFGRRFYGFASEAQMEPTVESEIFRALEKARLLVGGRKESCYSRCGRTDRGVSS 224
           IALKVMYFGRRFYGFASEAQMEPTVESEIFRAL+KARLLVGGRKESCYSRCGRTDRGVSS
Sbjct: 121 IALKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSS 180

Query: 225 VGQVIALYLRSNLKDPPNGENGNHNSEEHHDGEIDYVRVMNRVLPNDIRVLGWCTVPVGF 284
           VGQVIALYLRSNLKDPPNGENGNHN+EEHHDGEIDYVRV+NRVLPNDIRVLGWCTVPVGF
Sbjct: 181 VGQVIALYLRSNLKDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGF 240

Query: 285 SARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD 344
           SARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD
Sbjct: 241 SARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD 300

Query: 345 ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVYMVGQGLEDPDVVDILLDPLRTPRK 404
           ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVV+MVGQGLEDPDVVDILLDPLRTPRK
Sbjct: 301 ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPLRTPRK 360

Query: 405 PQYVMASENPLVLHSCEFEGVKFRISMPAFLLDAGEALQLHLTNEIRTYELQAAIFKEAL 464
           PQYVMASENPLVLHSCEFEGVKFR S      DAGEALQLHL+NEIRTYELQAAIFKEAL
Sbjct: 361 PQYVMASENPLVLHSCEFEGVKFRCST-----DAGEALQLHLSNEIRTYELQAAIFKEAL 420

Query: 465 LSCLPITNDESFPDNRKIKKKICHVPLFSRPTE 497
           LSCLPIT+DES  DNRKIKKKICHVPL SRPTE
Sbjct: 421 LSCLPITDDESLSDNRKIKKKICHVPLLSRPTE 446

BLAST of Cp4.1LG13g10920 vs. NCBI nr
Match: XP_022992675.1 (tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita maxima] >XP_022992676.1 tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita maxima])

HSP 1 Score: 852 bits (2202), Expect = 1.04e-309
Identity = 422/453 (93.16%), Postives = 435/453 (96.03%), Query Frame = 0

Query: 45  MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLST 104
           MDGAEHSLD+RG TGAMETTSGTDEFQPFD NLI NLQSQLRLLQGRVKELEEENSNLS+
Sbjct: 1   MDGAEHSLDHRGATGAMETTSGTDEFQPFDRNLIENLQSQLRLLQGRVKELEEENSNLSS 60

Query: 105 RLATCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAIRGSPKNGYNTRILDHCSKRY 164
           RLA+C CSERRMNFDDGSSSIEKSKKYGSLKDKTT+KKA+  +P  GYNTRILDHCSKRY
Sbjct: 61  RLASCYCSERRMNFDDGSSSIEKSKKYGSLKDKTTEKKAVEKTP--GYNTRILDHCSKRY 120

Query: 165 IALKVMYFGRRFYGFASEAQMEPTVESEIFRALEKARLLVGGRKESCYSRCGRTDRGVSS 224
           IALKVMYFGRRFYGFASEAQMEPTVESEIFRAL+KARLLVGGRKESCYSRCGRTD+GVSS
Sbjct: 121 IALKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDKGVSS 180

Query: 225 VGQVIALYLRSNLKDPPNGENGNHNSEEHHDGEIDYVRVMNRVLPNDIRVLGWCTVPVGF 284
           VGQVIALYLRSNLKDPPNGENGNHNS+EHHDGEIDYVRV+NRVLPNDIRVLGWCTVPVGF
Sbjct: 181 VGQVIALYLRSNLKDPPNGENGNHNSKEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGF 240

Query: 285 SARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD 344
           SARFSCLRR+YKYLFWGANLNISAME AGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD
Sbjct: 241 SARFSCLRRRYKYLFWGANLNISAMESAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD 300

Query: 345 ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVYMVGQGLEDPDVVDILLDPLRTPRK 404
           ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVV+MVGQGLEDPDVVDILLDP+RTPRK
Sbjct: 301 ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPVRTPRK 360

Query: 405 PQYVMASENPLVLHSCEFEGVKFRISMPAFLLDAGEALQLHLTNEIRTYELQAAIFKEAL 464
           PQYVMASENPLVLHSCEFEGVKFR S      DAGEALQLHLTNEIRTYELQAAIFKEAL
Sbjct: 361 PQYVMASENPLVLHSCEFEGVKFRCST-----DAGEALQLHLTNEIRTYELQAAIFKEAL 420

Query: 465 LSCLPITNDESFPDNRKIKKKICHVPLFSRPTE 497
           LSCLPITNDESF DNRKIKKKICHVPL SRPTE
Sbjct: 421 LSCLPITNDESFSDNRKIKKKICHVPLLSRPTE 446

BLAST of Cp4.1LG13g10920 vs. NCBI nr
Match: XP_022992677.1 (tRNA pseudouridine(38/39) synthase isoform X2 [Cucurbita maxima])

HSP 1 Score: 850 bits (2195), Expect = 1.21e-308
Identity = 421/453 (92.94%), Postives = 434/453 (95.81%), Query Frame = 0

Query: 45  MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLST 104
           MDGAEHSLD+RG TGAMETTSGTDEFQPFD NLI NLQSQLRLLQGRVK LEEENSNLS+
Sbjct: 1   MDGAEHSLDHRGATGAMETTSGTDEFQPFDRNLIENLQSQLRLLQGRVKVLEEENSNLSS 60

Query: 105 RLATCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAIRGSPKNGYNTRILDHCSKRY 164
           RLA+C CSERRMNFDDGSSSIEKSKKYGSLKDKTT+KKA+  +P  GYNTRILDHCSKRY
Sbjct: 61  RLASCYCSERRMNFDDGSSSIEKSKKYGSLKDKTTEKKAVEKTP--GYNTRILDHCSKRY 120

Query: 165 IALKVMYFGRRFYGFASEAQMEPTVESEIFRALEKARLLVGGRKESCYSRCGRTDRGVSS 224
           IALKVMYFGRRFYGFASEAQMEPTVESEIFRAL+KARLLVGGRKESCYSRCGRTD+GVSS
Sbjct: 121 IALKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDKGVSS 180

Query: 225 VGQVIALYLRSNLKDPPNGENGNHNSEEHHDGEIDYVRVMNRVLPNDIRVLGWCTVPVGF 284
           VGQVIALYLRSNLKDPPNGENGNHNS+EHHDGEIDYVRV+NRVLPNDIRVLGWCTVPVGF
Sbjct: 181 VGQVIALYLRSNLKDPPNGENGNHNSKEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGF 240

Query: 285 SARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD 344
           SARFSCLRR+YKYLFWGANLNISAME AGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD
Sbjct: 241 SARFSCLRRRYKYLFWGANLNISAMESAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD 300

Query: 345 ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVYMVGQGLEDPDVVDILLDPLRTPRK 404
           ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVV+MVGQGLEDPDVVDILLDP+RTPRK
Sbjct: 301 ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPVRTPRK 360

Query: 405 PQYVMASENPLVLHSCEFEGVKFRISMPAFLLDAGEALQLHLTNEIRTYELQAAIFKEAL 464
           PQYVMASENPLVLHSCEFEGVKFR S      DAGEALQLHLTNEIRTYELQAAIFKEAL
Sbjct: 361 PQYVMASENPLVLHSCEFEGVKFRCST-----DAGEALQLHLTNEIRTYELQAAIFKEAL 420

Query: 465 LSCLPITNDESFPDNRKIKKKICHVPLFSRPTE 497
           LSCLPITNDESF DNRKIKKKICHVPL SRPTE
Sbjct: 421 LSCLPITNDESFSDNRKIKKKICHVPLLSRPTE 446

BLAST of Cp4.1LG13g10920 vs. ExPASy TrEMBL
Match: A0A6J1FEX0 (tRNA pseudouridine(38/39) synthase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444871 PE=3 SV=1)

HSP 1 Score: 865 bits (2235), Expect = 4.77e-315
Identity = 427/453 (94.26%), Postives = 438/453 (96.69%), Query Frame = 0

Query: 45  MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLST 104
           MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQS+LRLLQGRVK LEEENSNLS+
Sbjct: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSRLRLLQGRVKVLEEENSNLSS 60

Query: 105 RLATCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAIRGSPKNGYNTRILDHCSKRY 164
           RLATCCCSERRMNFDDGSSSIEKS+KYGSLKDKTTKKKA+  +P  GYNTRILDHCSKRY
Sbjct: 61  RLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTP--GYNTRILDHCSKRY 120

Query: 165 IALKVMYFGRRFYGFASEAQMEPTVESEIFRALEKARLLVGGRKESCYSRCGRTDRGVSS 224
           IALKVMYFGRRFYGFASEAQMEPTVESEIFRAL+KARLLVGGRKESCYSRCGRTDRGVSS
Sbjct: 121 IALKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSS 180

Query: 225 VGQVIALYLRSNLKDPPNGENGNHNSEEHHDGEIDYVRVMNRVLPNDIRVLGWCTVPVGF 284
           VGQVIALYLRSNLKDPPNGENGNHN+EEHHDGEIDYVRV+NRVLPNDIRVLGWCTVPVGF
Sbjct: 181 VGQVIALYLRSNLKDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGF 240

Query: 285 SARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD 344
           SARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD
Sbjct: 241 SARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD 300

Query: 345 ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVYMVGQGLEDPDVVDILLDPLRTPRK 404
           ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVV+MVGQGLEDPDVVDILLDPLRTPRK
Sbjct: 301 ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPLRTPRK 360

Query: 405 PQYVMASENPLVLHSCEFEGVKFRISMPAFLLDAGEALQLHLTNEIRTYELQAAIFKEAL 464
           PQYVMASENPLVLHSCEFEGVKFR S      DAGEALQLHL+NEIRTYELQAAIFKEAL
Sbjct: 361 PQYVMASENPLVLHSCEFEGVKFRCST-----DAGEALQLHLSNEIRTYELQAAIFKEAL 420

Query: 465 LSCLPITNDESFPDNRKIKKKICHVPLFSRPTE 497
           LSCLPIT+DES  DNRKIKKKICHVPL SRPTE
Sbjct: 421 LSCLPITDDESLSDNRKIKKKICHVPLLSRPTE 446

BLAST of Cp4.1LG13g10920 vs. ExPASy TrEMBL
Match: A0A6J1JWD4 (tRNA pseudouridine(38/39) synthase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488950 PE=3 SV=1)

HSP 1 Score: 852 bits (2202), Expect = 5.03e-310
Identity = 422/453 (93.16%), Postives = 435/453 (96.03%), Query Frame = 0

Query: 45  MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLST 104
           MDGAEHSLD+RG TGAMETTSGTDEFQPFD NLI NLQSQLRLLQGRVKELEEENSNLS+
Sbjct: 1   MDGAEHSLDHRGATGAMETTSGTDEFQPFDRNLIENLQSQLRLLQGRVKELEEENSNLSS 60

Query: 105 RLATCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAIRGSPKNGYNTRILDHCSKRY 164
           RLA+C CSERRMNFDDGSSSIEKSKKYGSLKDKTT+KKA+  +P  GYNTRILDHCSKRY
Sbjct: 61  RLASCYCSERRMNFDDGSSSIEKSKKYGSLKDKTTEKKAVEKTP--GYNTRILDHCSKRY 120

Query: 165 IALKVMYFGRRFYGFASEAQMEPTVESEIFRALEKARLLVGGRKESCYSRCGRTDRGVSS 224
           IALKVMYFGRRFYGFASEAQMEPTVESEIFRAL+KARLLVGGRKESCYSRCGRTD+GVSS
Sbjct: 121 IALKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDKGVSS 180

Query: 225 VGQVIALYLRSNLKDPPNGENGNHNSEEHHDGEIDYVRVMNRVLPNDIRVLGWCTVPVGF 284
           VGQVIALYLRSNLKDPPNGENGNHNS+EHHDGEIDYVRV+NRVLPNDIRVLGWCTVPVGF
Sbjct: 181 VGQVIALYLRSNLKDPPNGENGNHNSKEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGF 240

Query: 285 SARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD 344
           SARFSCLRR+YKYLFWGANLNISAME AGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD
Sbjct: 241 SARFSCLRRRYKYLFWGANLNISAMESAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD 300

Query: 345 ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVYMVGQGLEDPDVVDILLDPLRTPRK 404
           ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVV+MVGQGLEDPDVVDILLDP+RTPRK
Sbjct: 301 ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPVRTPRK 360

Query: 405 PQYVMASENPLVLHSCEFEGVKFRISMPAFLLDAGEALQLHLTNEIRTYELQAAIFKEAL 464
           PQYVMASENPLVLHSCEFEGVKFR S      DAGEALQLHLTNEIRTYELQAAIFKEAL
Sbjct: 361 PQYVMASENPLVLHSCEFEGVKFRCST-----DAGEALQLHLTNEIRTYELQAAIFKEAL 420

Query: 465 LSCLPITNDESFPDNRKIKKKICHVPLFSRPTE 497
           LSCLPITNDESF DNRKIKKKICHVPL SRPTE
Sbjct: 421 LSCLPITNDESFSDNRKIKKKICHVPLLSRPTE 446

BLAST of Cp4.1LG13g10920 vs. ExPASy TrEMBL
Match: A0A6J1JQK1 (tRNA pseudouridine(38/39) synthase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111488950 PE=3 SV=1)

HSP 1 Score: 850 bits (2195), Expect = 5.86e-309
Identity = 421/453 (92.94%), Postives = 434/453 (95.81%), Query Frame = 0

Query: 45  MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLST 104
           MDGAEHSLD+RG TGAMETTSGTDEFQPFD NLI NLQSQLRLLQGRVK LEEENSNLS+
Sbjct: 1   MDGAEHSLDHRGATGAMETTSGTDEFQPFDRNLIENLQSQLRLLQGRVKVLEEENSNLSS 60

Query: 105 RLATCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAIRGSPKNGYNTRILDHCSKRY 164
           RLA+C CSERRMNFDDGSSSIEKSKKYGSLKDKTT+KKA+  +P  GYNTRILDHCSKRY
Sbjct: 61  RLASCYCSERRMNFDDGSSSIEKSKKYGSLKDKTTEKKAVEKTP--GYNTRILDHCSKRY 120

Query: 165 IALKVMYFGRRFYGFASEAQMEPTVESEIFRALEKARLLVGGRKESCYSRCGRTDRGVSS 224
           IALKVMYFGRRFYGFASEAQMEPTVESEIFRAL+KARLLVGGRKESCYSRCGRTD+GVSS
Sbjct: 121 IALKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDKGVSS 180

Query: 225 VGQVIALYLRSNLKDPPNGENGNHNSEEHHDGEIDYVRVMNRVLPNDIRVLGWCTVPVGF 284
           VGQVIALYLRSNLKDPPNGENGNHNS+EHHDGEIDYVRV+NRVLPNDIRVLGWCTVPVGF
Sbjct: 181 VGQVIALYLRSNLKDPPNGENGNHNSKEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGF 240

Query: 285 SARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD 344
           SARFSCLRR+YKYLFWGANLNISAME AGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD
Sbjct: 241 SARFSCLRRRYKYLFWGANLNISAMESAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD 300

Query: 345 ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVYMVGQGLEDPDVVDILLDPLRTPRK 404
           ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVV+MVGQGLEDPDVVDILLDP+RTPRK
Sbjct: 301 ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPVRTPRK 360

Query: 405 PQYVMASENPLVLHSCEFEGVKFRISMPAFLLDAGEALQLHLTNEIRTYELQAAIFKEAL 464
           PQYVMASENPLVLHSCEFEGVKFR S      DAGEALQLHLTNEIRTYELQAAIFKEAL
Sbjct: 361 PQYVMASENPLVLHSCEFEGVKFRCST-----DAGEALQLHLTNEIRTYELQAAIFKEAL 420

Query: 465 LSCLPITNDESFPDNRKIKKKICHVPLFSRPTE 497
           LSCLPITNDESF DNRKIKKKICHVPL SRPTE
Sbjct: 421 LSCLPITNDESFSDNRKIKKKICHVPLLSRPTE 446

BLAST of Cp4.1LG13g10920 vs. ExPASy TrEMBL
Match: A0A6J1FEX6 (tRNA pseudouridine(38/39) synthase isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111444871 PE=3 SV=1)

HSP 1 Score: 761 bits (1965), Expect = 3.68e-275
Identity = 371/386 (96.11%), Postives = 380/386 (98.45%), Query Frame = 0

Query: 45  MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLST 104
           MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQS+LRLLQGRVK LEEENSNLS+
Sbjct: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSRLRLLQGRVKVLEEENSNLSS 60

Query: 105 RLATCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAIRGSPKNGYNTRILDHCSKRY 164
           RLATCCCSERRMNFDDGSSSIEKS+KYGSLKDKTTKKKA+  +P  GYNTRILDHCSKRY
Sbjct: 61  RLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTP--GYNTRILDHCSKRY 120

Query: 165 IALKVMYFGRRFYGFASEAQMEPTVESEIFRALEKARLLVGGRKESCYSRCGRTDRGVSS 224
           IALKVMYFGRRFYGFASEAQMEPTVESEIFRAL+KARLLVGGRKESCYSRCGRTDRGVSS
Sbjct: 121 IALKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSS 180

Query: 225 VGQVIALYLRSNLKDPPNGENGNHNSEEHHDGEIDYVRVMNRVLPNDIRVLGWCTVPVGF 284
           VGQVIALYLRSNLKDPPNGENGNHN+EEHHDGEIDYVRV+NRVLPNDIRVLGWCTVPVGF
Sbjct: 181 VGQVIALYLRSNLKDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGF 240

Query: 285 SARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD 344
           SARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD
Sbjct: 241 SARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD 300

Query: 345 ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVYMVGQGLEDPDVVDILLDPLRTPRK 404
           ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVV+MVGQGLEDPDVVDILLDPLRTPRK
Sbjct: 301 ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPLRTPRK 360

Query: 405 PQYVMASENPLVLHSCEFEGVKFRIS 430
           PQYVMASENPLVLHSCEFEGVKFR S
Sbjct: 361 PQYVMASENPLVLHSCEFEGVKFRCS 384

BLAST of Cp4.1LG13g10920 vs. ExPASy TrEMBL
Match: A0A6J1JU85 (tRNA pseudouridine(38/39) synthase isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111488950 PE=3 SV=1)

HSP 1 Score: 743 bits (1917), Expect = 7.49e-268
Identity = 363/386 (94.04%), Postives = 376/386 (97.41%), Query Frame = 0

Query: 45  MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLST 104
           MDGAEHSLD+RG TGAMETTSGTDEFQPFD NLI NLQSQLRLLQGRVKELEEENSNLS+
Sbjct: 1   MDGAEHSLDHRGATGAMETTSGTDEFQPFDRNLIENLQSQLRLLQGRVKELEEENSNLSS 60

Query: 105 RLATCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAIRGSPKNGYNTRILDHCSKRY 164
           RLA+C CSERRMNFDDGSSSIEKSKKYGSLKDKTT+KKA+  +P  GYNTRILDHCSKRY
Sbjct: 61  RLASCYCSERRMNFDDGSSSIEKSKKYGSLKDKTTEKKAVEKTP--GYNTRILDHCSKRY 120

Query: 165 IALKVMYFGRRFYGFASEAQMEPTVESEIFRALEKARLLVGGRKESCYSRCGRTDRGVSS 224
           IALKVMYFGRRFYGFASEAQMEPTVESEIFRAL+KARLLVGGRKESCYSRCGRTD+GVSS
Sbjct: 121 IALKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDKGVSS 180

Query: 225 VGQVIALYLRSNLKDPPNGENGNHNSEEHHDGEIDYVRVMNRVLPNDIRVLGWCTVPVGF 284
           VGQVIALYLRSNLKDPPNGENGNHNS+EHHDGEIDYVRV+NRVLPNDIRVLGWCTVPVGF
Sbjct: 181 VGQVIALYLRSNLKDPPNGENGNHNSKEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGF 240

Query: 285 SARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD 344
           SARFSCLRR+YKYLFWGANLNISAME AGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD
Sbjct: 241 SARFSCLRRRYKYLFWGANLNISAMESAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFD 300

Query: 345 ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVYMVGQGLEDPDVVDILLDPLRTPRK 404
           ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVV+MVGQGLEDPDVVDILLDP+RTPRK
Sbjct: 301 ISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPVRTPRK 360

Query: 405 PQYVMASENPLVLHSCEFEGVKFRIS 430
           PQYVMASENPLVLHSCEFEGVKFR S
Sbjct: 361 PQYVMASENPLVLHSCEFEGVKFRCS 384

BLAST of Cp4.1LG13g10920 vs. TAIR 10
Match: AT1G34150.1 (Pseudouridine synthase family protein )

HSP 1 Score: 453.4 bits (1165), Expect = 2.5e-127
Identity = 224/421 (53.21%), Postives = 294/421 (69.83%), Query Frame = 0

Query: 82  QSQLRLLQGRVKELEEENSNLSTRLATCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKK 141
           +++L  L+ RVKELE EN+ L +++++C C +  +  D   S                ++
Sbjct: 22  ETELVFLRNRVKELEVENAKLLSQVSSCQCQQMEVKHDRSLSD----------SSSLVRR 81

Query: 142 KAIRGSPKNGYNTRILDHCSKRYIALKVMYFGRRFYGFASEAQMEPTVESEIFRALEKAR 201
           + +R   KN   + ++   SKRY+ALK+MYFG+RFYGF++EAQMEP++ESE+F+ALE+ R
Sbjct: 82  RRVRKGDKNSIPSHLI---SKRYVALKIMYFGKRFYGFSAEAQMEPSIESEVFKALERTR 141

Query: 202 LLVGGRKESCYSRCGRTDRGVSSVGQVIALYLRSNLKDPPNGENGNHNSEEHHDGEIDYV 261
           LLVG +K+SCYSRCGRTD+GVSS GQVIAL+LRS LK PP       N       E DYV
Sbjct: 142 LLVGDKKDSCYSRCGRTDKGVSSTGQVIALFLRSRLKSPPGDSKAQVNGRTGERPEYDYV 201

Query: 262 RVMNRVLPNDIRVLGWCTVPVGFSARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHD 321
           RV+NR LP+DIRV+GW   P+ F ARFSC  R+YKY FW  NLN+SAM+ AGKKF+GEHD
Sbjct: 202 RVLNRALPDDIRVIGWSPAPIDFHARFSCYAREYKYFFWRQNLNLSAMDFAGKKFIGEHD 261

Query: 322 FRNFCKMDAANVHNYRRCIMSFDISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVYM 381
           FRNFCKMD ANVH Y R +  F++SPC    EG+ L  F + GSAFLWHQ+RCMVAV++M
Sbjct: 262 FRNFCKMDVANVHCYTRRVTFFEVSPCQNSHEGDQLCTFTMRGSAFLWHQIRCMVAVLFM 321

Query: 382 VGQGLEDPDVVDILLDPLRTPRKPQYVMASENPLVLHSCEFEGVKFRISMPAFLLDAGEA 441
           +GQG+E  DV+D LLD  +TPRKPQY++ASE PLVL +CEFE V F I  P     A E+
Sbjct: 322 IGQGVESVDVIDTLLDTKKTPRKPQYLLASEIPLVLRTCEFENVDF-ICSPG----AAES 381

Query: 442 LQLHLTNEIRTYELQAAIFKEALLSCLPITNDESFPDN-----RKIKKKICHVPLFSRPT 498
           L+ H  NE  TY+L++ I++EAL +CLP++N+ S  ++      K KK+  HVPL SRPT
Sbjct: 382 LRSHFKNESLTYQLESVIYQEALRNCLPLSNNVSTEESSCNGVEKKKKRAEHVPLLSRPT 424

BLAST of Cp4.1LG13g10920 vs. TAIR 10
Match: AT3G06950.1 (Pseudouridine synthase family protein )

HSP 1 Score: 89.0 bits (219), Expect = 1.2e-17
Identity = 85/292 (29.11%), Postives = 132/292 (45.21%), Query Frame = 0

Query: 167 LKVMYFGRRFYGFASEAQMEPTVESEIFRALEKARLLVGGRKESCYSRCGRTDRGVSSVG 226
           L + Y G RF G+  + +  PT++S + +AL +   L  GRKE      GRTD GV + G
Sbjct: 45  LVIAYDGTRFAGWQYQ-ESPPTIQSMLEKALIQITEL--GRKELQLIGAGRTDAGVHAWG 104

Query: 227 QVIALYLRSNLKDPPNGENGNHNSEEHHDGEIDYVRVMNRVLPNDIRVLGWCTVPVGFSA 286
           QV      ++   P N           +     +   +N +LP DIRV         F A
Sbjct: 105 QV------AHFVTPFN-----------YTSLDSFHAALNGLLPKDIRVRELSAAVPEFHA 164

Query: 287 RFSCLRRKYKYLFW----------------GANLNISAMERAGKKFVGEHDFRNFCKMDA 346
           RFS   + Y+Y  +                   LN S M  A   FVG+HDF  F     
Sbjct: 165 RFSASSKVYRYQIYNDTFMDPFQRHWAYHCAYKLNASKMREAANLFVGKHDFSAFANATR 224

Query: 347 AN-VHNYRRCIMSFDISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVYMVGQGLEDP 406
            + V +  + I  FD+    ++M G+LL    +EGS FL+ QVR MVA++  +G+   D 
Sbjct: 225 EDGVPDPLKTISRFDV----IQM-GSLL-QLEVEGSGFLYRQVRNMVALLIQIGKEALDS 284

Query: 407 DVVDILLD--PLRTPRKPQYVMASENPLVLHSCEFEGVKFRISMPAFLLDAG 440
           D+V ++L+    R   K   + AS + L L S +++    ++ +   +  +G
Sbjct: 285 DIVPMILETKDRRVLAKYTSLPASPHGLCLVSVKYKEDHLKLPLDCPVTSSG 310

BLAST of Cp4.1LG13g10920 vs. TAIR 10
Match: AT1G76120.1 (Pseudouridine synthase family protein )

HSP 1 Score: 53.5 bits (127), Expect = 5.8e-07
Identity = 36/114 (31.58%), Postives = 55/114 (48.25%), Query Frame = 0

Query: 185 MEPTVESEIFRALEKARLLVGGRKESCYSRCGRTDRGVSSVGQVIALYLRSNLKDPPNGE 244
           +E  +E  +F A      + G  K   ++R  RTD+GVS+VGQV++      + DP    
Sbjct: 47  IEGELEEALFHAGAVPESIRGKPKLYDFARSARTDKGVSAVGQVVS---GRFIVDP---- 106

Query: 245 NGNHNSEEHHDGEIDYVRVMNRVLPNDIRVLGWCTVPVGFSARFSCLRRKYKYL 299
                        + +V  +N  LPN IR+ G+  V   FS++  C RR+Y YL
Sbjct: 107 -------------LGFVNRLNSNLPNQIRIFGYKHVTPSFSSKKFCDRRRYVYL 140

BLAST of Cp4.1LG13g10920 vs. TAIR 10
Match: AT1G20370.1 (Pseudouridine synthase family protein )

HSP 1 Score: 53.5 bits (127), Expect = 5.8e-07
Identity = 42/143 (29.37%), Postives = 66/143 (46.15%), Query Frame = 0

Query: 162 KRYIALKVMYFGRRFYGFASEAQMEPTVESEIFRALEKARLLVGGRKESC----YSRCGR 221
           +R +A+   + G  + G       + T+E E+  AL  A  +    +       ++R  R
Sbjct: 57  RRKVAIVFAFCGVGYQGMQKNPGAK-TIEGELEEALFHAGAVPDADRNKPRNYEWARSAR 116

Query: 222 TDRGVSSVGQVIA--LYLRSNLKDPPNGENGNHNSEEHHDGEIDYVRVMNRVLPNDIRVL 281
           TD+GVS+VGQV++   Y+     DPP                  +V  +N  LP+ IRV 
Sbjct: 117 TDKGVSAVGQVVSGRFYV-----DPPG-----------------FVERLNSKLPDQIRVF 176

Query: 282 GWCTVPVGFSARFSCLRRKYKYL 299
           G+  V   FS++  C RR+Y YL
Sbjct: 177 GYKRVAPSFSSKKFCDRRRYVYL 176

BLAST of Cp4.1LG13g10920 vs. TAIR 10
Match: AT1G76120.2 (Pseudouridine synthase family protein )

HSP 1 Score: 53.5 bits (127), Expect = 5.8e-07
Identity = 36/114 (31.58%), Postives = 55/114 (48.25%), Query Frame = 0

Query: 185 MEPTVESEIFRALEKARLLVGGRKESCYSRCGRTDRGVSSVGQVIALYLRSNLKDPPNGE 244
           +E  +E  +F A      + G  K   ++R  RTD+GVS+VGQV++      + DP    
Sbjct: 47  IEGELEEALFHAGAVPESIRGKPKLYDFARSARTDKGVSAVGQVVS---GRFIVDP---- 106

Query: 245 NGNHNSEEHHDGEIDYVRVMNRVLPNDIRVLGWCTVPVGFSARFSCLRRKYKYL 299
                        + +V  +N  LPN IR+ G+  V   FS++  C RR+Y YL
Sbjct: 107 -------------LGFVNRLNSNLPNQIRIFGYKHVTPSFSSKKFCDRRRYVYL 140

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9JI381.3e-6737.50tRNA pseudouridine(38/39) synthase OS=Mus musculus OX=10090 GN=Pus3 PE=1 SV=2[more]
Q3SX071.8e-6642.23tRNA pseudouridine(38/39) synthase OS=Bos taurus OX=9913 GN=PUS3 PE=2 SV=1[more]
Q9BZE22.4e-6639.64tRNA pseudouridine(38/39) synthase OS=Homo sapiens OX=9606 GN=PUS3 PE=1 SV=3[more]
P311159.7e-6039.50tRNA pseudouridine(38/39) synthase OS=Saccharomyces cerevisiae (strain ATCC 2045... [more]
Q9UTB31.9e-5542.70Putative tRNA pseudouridine synthase C25B8.05 OS=Schizosaccharomyces pombe (stra... [more]
Match NameE-valueIdentityDescription
KAG6578858.10.095.53putative F-actin-capping protein subunit beta, partial [Cucurbita argyrosperma s... [more]
XP_023551060.10.097.13tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita pepo subsp. pepo] >XP_0... [more]
XP_022938732.19.85e-31594.26tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita moschata] >XP_022938734... [more]
XP_022992675.11.04e-30993.16tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita maxima] >XP_022992676.1... [more]
XP_022992677.11.21e-30892.94tRNA pseudouridine(38/39) synthase isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1FEX04.77e-31594.26tRNA pseudouridine(38/39) synthase isoform X1 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1JWD45.03e-31093.16tRNA pseudouridine(38/39) synthase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1JQK15.86e-30992.94tRNA pseudouridine(38/39) synthase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1FEX63.68e-27596.11tRNA pseudouridine(38/39) synthase isoform X3 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1JU857.49e-26894.04tRNA pseudouridine(38/39) synthase isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT1G34150.12.5e-12753.21Pseudouridine synthase family protein [more]
AT3G06950.11.2e-1729.11Pseudouridine synthase family protein [more]
AT1G76120.15.8e-0731.58Pseudouridine synthase family protein [more]
AT1G20370.15.8e-0729.37Pseudouridine synthase family protein [more]
AT1G76120.25.8e-0731.58Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 78..105
NoneNo IPR availablePIRSRPIRSR001430-2PIRSR001430-2coord: 208..417
e-value: 8.3E-34
score: 114.9
NoneNo IPR availablePANTHERPTHR11142:SF5TRNA PSEUDOURIDINE(38/39) SYNTHASEcoord: 82..498
IPR020097Pseudouridine synthase I, TruA, alpha/beta domainPFAMPF01416PseudoU_synth_1coord: 313..423
e-value: 5.5E-28
score: 97.6
IPR020095Pseudouridine synthase I, TruA, C-terminalGENE3D3.30.70.660coord: 291..468
e-value: 2.9E-39
score: 136.6
IPR001406Pseudouridine synthase I, TruATIGRFAMTIGR00071TIGR00071coord: 164..409
e-value: 1.7E-50
score: 169.8
IPR001406Pseudouridine synthase I, TruAPANTHERPTHR11142PSEUDOURIDYLATE SYNTHASEcoord: 82..498
IPR001406Pseudouridine synthase I, TruAHAMAPMF_00171TruAcoord: 163..422
score: 23.41695
IPR020094Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminalGENE3D3.30.70.580coord: 154..290
e-value: 3.6E-32
score: 112.9
IPR041707Pseudouridine synthase Pus3-likeCDDcd02569PseudoU_synth_ScPus3coord: 167..422
e-value: 3.95071E-135
score: 389.723
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 162..432

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG13g10920.1Cp4.1LG13g10920.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0008033 tRNA processing
biological_process GO:0009451 RNA modification
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding