Cp4.1LG13g09430 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG13g09430
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	receptor-like cytoplasmic kinase 176
Location	Cp4.1LG13: 7518272 .. 7519777 (+)
RNA-Seq Expression	Cp4.1LG13g09430
Synteny	Cp4.1LG13g09430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTATATATAATGCCTCCTACACAGGCCTGAGCTCCAAGGATTCAAGAGATGATAAAGGTAATTCAATCAGCAAGTCTTCATCGGTCTCGACCCCGCGGACTCCACGAACAGAGGGCGAGATCTTGCAGTCCTCCAACTTGAAAAACTTCCCCTACAATGAGCTTAAAACCGCCACTCGAAACTTCAGGCCTGATAGTGTGTTGGGTGAGGGTGGTTTTGGTTCTGTCTTTAAAGGGTGGATTGATGAGCAGTCCTTTGCAGCTACCAAGCCCGGGACTGGCTTGGTCATTGCAGTGAAAAGGCTCAACCAAGAAGGCTTTCAGGGTCACAGAGAATGGCTGGTTAGTATCTCTCTATCCATTGACGTGTACTCTTCATGCTACTCTGCTTTTGGAATGGATTCATTTATGGATGTCTCAATTTACAGACAGAAATAGATTACCTTGGTCAGCTGCATCATCCTAATCTTGTGAGATTGATTGGCTTTTGTTTAGAGGACGAGCATCGACTTCTCGTTTACGAGTTTATGCCTCGAGGCAGCTTGGAAAACCATCTATTTAGGAGTAAGCTATTGACTTATTTCACCTGTTGAATTGGTATTGCAAGAATGACTTAGATTCTCCTTCTATGTCTTTGGTGGATCAGGGAGTTCTCGGTTTCAACCTCTATCATGGAGCCTGAGAATGAAAGTTGCCCTTGGTGCAGCTAAAGGACTAGCTTTTCTACATAGCGACGAAGCGAAAGTAATATACCGAGACTTCAAAAGCTCGAATATCCTGCTCGACTCAGTATGTGAGACAATTCAACCTCTTCAAGCTTATGTTTCTGACATATTTGTTGTTGATCTCATTTGTATTGGACTTTGAAATGACAGAGCTATAATGCAAAACTCTCTGATTTTGGATTAGCCAAGGATGGTCCTACAGGTGATAAAAGCTATGTTTCCACGAGAGTTATGGGAACATTTGGCTATGCTGCTCCTGAATATATGATCACAGGTAGCTTTCTAGTTGAGTTTGACTATGACTATGACTACTAGTTATTTAACGACGTCCTCTTCTTTTGAAGGTCATCTCACTTCGAAGAGCGATGTTTATAGTTTTGGAGTTGTTCTTCTTGAGATCCTATCGGGGAGACGGGCTATAGACAAAAACCGACCATCTGGAGAACATAATCTTGTGGAATGGGCGAAACCTTACCTCGCTAGCAAACGAAGAATTCTTCGAATATTGGATGCACGTATCGAAGGCCAGTATTCGACAGGAGGAGCACTGAAAGCAGCTAAACTTGCCATTCAATGCATATCAACTGAACCCAAGTTAAGGCCAAACATGAATGTGGTGGTGAAAGCATTGGAGCAAATTCAGGATTCCAGTGAAACAAATGGATCAAGGGGTGGCCCTGTAAGCAAACCCCTAAAGAACTCCAGTCAAGGCCGTGGCTCCACAAATAACAAAGCTGTTTCTTATCCTAGACCATCAGCTTCAGTACTAAATCTTTAG

mRNA sequence

ATGGTTATATATAATGCCTCCTACACAGGCCTGAGCTCCAAGGATTCAAGAGATGATAAAGGTAATTCAATCAGCAAGTCTTCATCGGTCTCGACCCCGCGGACTCCACGAACAGAGGGCGAGATCTTGCAGTCCTCCAACTTGAAAAACTTCCCCTACAATGAGCTTAAAACCGCCACTCGAAACTTCAGGCCTGATAGTGTGTTGGGTGAGGGTGGTTTTGGTTCTGTCTTTAAAGGGTGGATTGATGAGCAGTCCTTTGCAGCTACCAAGCCCGGGACTGGCTTGGTCATTGCAGTGAAAAGGCTCAACCAAGAAGGCTTTCAGGGTCACAGAGAATGGCTGGTTAGTATCTCTCTATCCATTGACGTATTCTCCTTCTATGTCTTTGGTGGATCAGGGAGTTCTCGGTTTCAACCTCTATCATGGAGCCTGAGAATGAAAGTTGCCCTTGGTGCAGCTAAAGGACTAGCTTTTCTACATAGCGACGAAGCGAAAGTAATATACCGAGACTTCAAAAGCTCGAATATCCTGCTCGACTCAAGCTATAATGCAAAACTCTCTGATTTTGGATTAGCCAAGGATGGTCCTACAGGTGATAAAAGCTATGTTTCCACGAGAGTTATGGGAACATTTGGCTATGCTGCTCCTGAATATATGATCACAGGAGGAGCACTGAAAGCAGCTAAACTTGCCATTCAATGCATATCAACTGAACCCAAGTTAAGGCCAAACATGAATGTGGTGGTGAAAGCATTGGAGCAAATTCAGGATTCCAGTGAAACAAATGGATCAAGGGGTGGCCCTGTAAGCAAACCCCTAAAGAACTCCAGTCAAGGCCGTGGCTCCACAAATAACAAAGCTGTTTCTTATCCTAGACCATCAGCTTCAGTACTAAATCTTTAG

Coding sequence (CDS)

ATGGTTATATATAATGCCTCCTACACAGGCCTGAGCTCCAAGGATTCAAGAGATGATAAAGGTAATTCAATCAGCAAGTCTTCATCGGTCTCGACCCCGCGGACTCCACGAACAGAGGGCGAGATCTTGCAGTCCTCCAACTTGAAAAACTTCCCCTACAATGAGCTTAAAACCGCCACTCGAAACTTCAGGCCTGATAGTGTGTTGGGTGAGGGTGGTTTTGGTTCTGTCTTTAAAGGGTGGATTGATGAGCAGTCCTTTGCAGCTACCAAGCCCGGGACTGGCTTGGTCATTGCAGTGAAAAGGCTCAACCAAGAAGGCTTTCAGGGTCACAGAGAATGGCTGGTTAGTATCTCTCTATCCATTGACGTATTCTCCTTCTATGTCTTTGGTGGATCAGGGAGTTCTCGGTTTCAACCTCTATCATGGAGCCTGAGAATGAAAGTTGCCCTTGGTGCAGCTAAAGGACTAGCTTTTCTACATAGCGACGAAGCGAAAGTAATATACCGAGACTTCAAAAGCTCGAATATCCTGCTCGACTCAAGCTATAATGCAAAACTCTCTGATTTTGGATTAGCCAAGGATGGTCCTACAGGTGATAAAAGCTATGTTTCCACGAGAGTTATGGGAACATTTGGCTATGCTGCTCCTGAATATATGATCACAGGAGGAGCACTGAAAGCAGCTAAACTTGCCATTCAATGCATATCAACTGAACCCAAGTTAAGGCCAAACATGAATGTGGTGGTGAAAGCATTGGAGCAAATTCAGGATTCCAGTGAAACAAATGGATCAAGGGGTGGCCCTGTAAGCAAACCCCTAAAGAACTCCAGTCAAGGCCGTGGCTCCACAAATAACAAAGCTGTTTCTTATCCTAGACCATCAGCTTCAGTACTAAATCTTTAG

Protein sequence

MVIYNASYTGLSSKDSRDDKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRPDSVLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLVSISLSIDVFSFYVFGGSGSSRFQPLSWSLRMKVALGAAKGLAFLHSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAPEYMITGGALKAAKLAIQCISTEPKLRPNMNVVVKALEQIQDSSETNGSRGGPVSKPLKNSSQGRGSTNNKAVSYPRPSASVLNL

Homology

[Close]

BLAST of Cp4.1LG13g09430 vs. ExPASy Swiss-Prot
Match: Q65XV8 (Receptor-like cytoplasmic kinase 176 OS=Oryza sativa subsp. japonica OX=39947 GN=RLCK176 PE=1 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 4.0e-82
Identity = 184/376 (48.94%), Postives = 219/376 (58.24%), Query Frame = 0


Query: 12  SSKDSRDDKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRPDSVLGE 71
           SS      K  S S  S+ S P TPR+E EIL+++N+K F +NEL+TATRNFRPDSVLGE
Sbjct: 19  SSPSGGTSKYASNSSVSAASVPPTPRSEDEILEAANVKAFAFNELRTATRNFRPDSVLGE 78

Query: 72  GGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLVSISL----------- 131
           GGFGSVFKGWIDE++ A TKPGTG+VIAVK+LNQEG QGHREWL  ++            
Sbjct: 79  GGFGSVFKGWIDEKTLAPTKPGTGMVIAVKKLNQEGHQGHREWLAEVNYLGQLSHPYLVR 138

Query: 132 ----------SIDVFSFYVFGG------SGSSRFQPLSWSLRMKVALGAAKGLAFLHSDE 191
                      + V+ F   G         S+ FQPLSW+LRMK+ALGAAKGLAFLHSD+
Sbjct: 139 LVGYCVEDEQRLLVYEFMPRGSLENHLFRRSTHFQPLSWNLRMKIALGAAKGLAFLHSDK 198

Query: 192 AKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAPEYMITG- 251
            KVIYRDFK+SN+LLD++Y+AKLSDFGLAKDGPTGDKS+VSTRVMGT+GYAAPEY+ TG 
Sbjct: 199 VKVIYRDFKTSNVLLDANYDAKLSDFGLAKDGPTGDKSHVSTRVMGTYGYAAPEYLATGH 258

Query: 252 ------------------------------------------------------------ 296
                                                                       
Sbjct: 259 LTTKSDVYSFGVVLLEMLSGRRALDKNRPTGEHNLVEWARPYLMSKRRIFRILDARLGGQ 318

[Close]

BLAST of Cp4.1LG13g09430 vs. ExPASy Swiss-Prot
Match: P46573 (Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 GN=PBL10 PE=1 SV=2)

HSP 1 Score: 298.1 bits (762), Expect = 1.1e-79
Identity = 190/397 (47.86%), Postives = 222/397 (55.92%), Query Frame = 0


Query: 10  GLSSKDSRDDKGNSI-SKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRPDSV 69
           G S K    +  +S+ SKSSSVS    PRTEGEILQS NLK+F + ELK ATRNFRPDSV
Sbjct: 15  GASPKYMSSEANDSLGSKSSSVSIRTNPRTEGEILQSPNLKSFTFAELKAATRNFRPDSV 74

Query: 70  LGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLVSISLSIDVFS-- 129
           LGEGGFGSVFKGWIDEQ+  A+KPGTG+VIAVK+LNQ+G+QGH+EWL  ++  +  FS  
Sbjct: 75  LGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKKLNQDGWQGHQEWLAEVNY-LGQFSHP 134

Query: 130 -----------------FYVFGGSGS---------SRFQPLSWSLRMKVALGAAKGLAFL 189
                             Y F   GS         S FQPLSW+LR+KVALGAAKGLAFL
Sbjct: 135 NLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQPLSWTLRLKVALGAAKGLAFL 194

Query: 190 HSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAPEYM 249
           H+ E  VIYRDFK+SNILLDS YNAKLSDFGLAKDGPTGDKS+VSTR+MGT+GYAAPEY+
Sbjct: 195 HNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYL 254

Query: 250 ITG--------------------------------------------------------- 300
            TG                                                         
Sbjct: 255 ATGHLTTKSDVYSYGVVLLEVLSGRRAVDKNRPPGEQKLVEWARPLLANKRKLFRVIDNR 314

[Close]

BLAST of Cp4.1LG13g09430 vs. ExPASy Swiss-Prot
Match: Q06548 (Probable serine/threonine-protein kinase PBL9 OS=Arabidopsis thaliana OX=3702 GN=PBL9 PE=1 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 1.4e-79
Identity = 188/399 (47.12%), Postives = 223/399 (55.89%), Query Frame = 0


Query: 6   ASYTGLSSKDSRDDKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRP 65
           A  +G S+K    D G+  SK+SSVS   +PRTEGEILQS NLK+F + ELK+ATRNFRP
Sbjct: 11  AESSGASTKYDAKDIGSLGSKASSVSVRPSPRTEGEILQSPNLKSFSFAELKSATRNFRP 70

Query: 66  DSVLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLVSISLSIDVF 125
           DSVLGEGGFG VFKGWIDE+S  A++PGTGLVIAVK+LNQ+G+QGH+EWL  ++  +  F
Sbjct: 71  DSVLGEGGFGCVFKGWIDEKSLTASRPGTGLVIAVKKLNQDGWQGHQEWLAEVNY-LGQF 130

Query: 126 S-------------------FYVFGGSGSSR---------FQPLSWSLRMKVALGAAKGL 185
           S                    Y F   GS           FQPLSW LR+KVALGAAKGL
Sbjct: 131 SHRHLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGLYFQPLSWKLRLKVALGAAKGL 190

Query: 186 AFLHSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAP 245
           AFLHS E +VIYRDFK+SNILLDS YNAKLSDFGLAKDGP GDKS+VSTRVMGT GYAAP
Sbjct: 191 AFLHSSETRVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRVMGTHGYAAP 250

Query: 246 EYMITG------------------------------------------------------ 300
           EY+ TG                                                      
Sbjct: 251 EYLATGHLTTKSDVYSFGVVLLELLSGRRAVDKNRPSGERNLVEWAKPYLVNKRKIFRVI 310

[Close]

BLAST of Cp4.1LG13g09430 vs. ExPASy Swiss-Prot
Match: P43293 (Probable serine/threonine-protein kinase PBL11 OS=Arabidopsis thaliana OX=3702 GN=PBL11 PE=1 SV=2)

HSP 1 Score: 278.5 bits (711), Expect = 9.0e-74
Identity = 179/384 (46.61%), Postives = 217/384 (56.51%), Query Frame = 0


Query: 6   ASYTGLSSKD-SRD-DKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNF 65
           AS T LSSK  SRD  KG     SS+ S    PRTEGEILQ++NLKNF  +ELK+ATRNF
Sbjct: 14  ASSTWLSSKFLSRDGSKG-----SSTASFSYMPRTEGEILQNANLKNFSLSELKSATRNF 73

Query: 66  RPDSVLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLVSISL--- 125
           RPDSV+GEGGFG VFKGWIDE S A +KPGTG+VIAVKRLNQEGFQGHREWL  I+    
Sbjct: 74  RPDSVVGEGGFGCVFKGWIDESSLAPSKPGTGIVIAVKRLNQEGFQGHREWLAEINYLGQ 133

Query: 126 ------------------SIDVFSFYVFGGSGSSRF------QPLSWSLRMKVALGAAKG 185
                              + V+ F   G   +  F      QPLSW+ R+++ALGAA+G
Sbjct: 134 LDHPNLVKLIGYCLEEEHRLLVYEFMTRGSLENHLFRRGTFYQPLSWNTRVRMALGAARG 193

Query: 186 LAFLHSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAA 245
           LAFLH+ + +VIYRDFK+SNILLDS+YNAKLSDFGLA+DGP GD S+VSTRVMGT GYAA
Sbjct: 194 LAFLHNAQPQVIYRDFKASNILLDSNYNAKLSDFGLARDGPMGDNSHVSTRVMGTQGYAA 253

Query: 246 PEYMITG----------------------------------------------------- 296
           PEY+ TG                                                     
Sbjct: 254 PEYLATGHLSVKSDVYSFGVVLLELLSGRRAIDKNQPVGEHNLVDWARPYLTNKRRLLRV 313

[Close]

BLAST of Cp4.1LG13g09430 vs. ExPASy Swiss-Prot
Match: O48814 (Serine/threonine-protein kinase BIK1 OS=Arabidopsis thaliana OX=3702 GN=BIK1 PE=1 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 1.9e-71
Identity = 164/345 (47.54%), Postives = 197/345 (57.10%), Query Frame = 0


Query: 16  SRDDKGNSISKSSSVST-PRTPRTEGEILQSSNLKNFPYNELKTATRNFRPDSVLGEGGF 75
           S D  G S+S   S ST     +TEGEIL S+ +K+F +NELK ATRNFRPDSV+GEGGF
Sbjct: 19  SSDLYGLSLSSRKSSSTVAAAQKTEGEILSSTPVKSFTFNELKLATRNFRPDSVIGEGGF 78

Query: 76  GSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLVSISL--------SIDVFS 135
           G VFKGW+DE +   TKPGTGLVIAVK+LNQEGFQGHREWL  I+          + +  
Sbjct: 79  GCVFKGWLDESTLTPTKPGTGLVIAVKKLNQEGFQGHREWLTEINYLGQLSHPNLVKLIG 138

Query: 136 F----------YVFGGSGS---------SRFQPLSWSLRMKVALGAAKGLAFLHSDEAKV 195
           +          Y F   GS         + F+PL W LR+ VAL AAKGLAFLHSD  KV
Sbjct: 139 YCLEDEHRLLVYEFMQKGSLENHLFRRGAYFKPLPWFLRVNVALDAAKGLAFLHSDPVKV 198

Query: 196 IYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAPEYMITG---- 255
           IYRD K+SNILLD+ YNAKLSDFGLA+DGP GD SYVSTRVMGT+GYAAPEYM +G    
Sbjct: 199 IYRDIKASNILLDADYNAKLSDFGLARDGPMGDLSYVSTRVMGTYGYAAPEYMSSGHLNA 258

Query: 256 ------------------------------------------------------------ 264
                                                                       
Sbjct: 259 RSDVYSFGVLLLEILSGKRALDHNRPAKEENLVDWARPYLTSKRKVLLIVDNRLDTQYLP 318

[Close]

BLAST of Cp4.1LG13g09430 vs. NCBI nr
Match: XP_023549888.1 (receptor-like cytoplasmic kinase 176 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 507 bits (1306), Expect = 3.46e-178
Identity = 286/393 (72.77%), Postives = 288/393 (73.28%), Query Frame = 0


Query: 1   MVIYNASYTGLSSKDSRDDKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTAT 60
           MVIYNASYTGLSSKDSRDDKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTAT
Sbjct: 1   MVIYNASYTGLSSKDSRDDKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTAT 60

Query: 61  RNFRPDSVLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLVSISL 120
           RNFRPDSVLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWL  I  
Sbjct: 61  RNFRPDSVLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLTEIDY 120

Query: 121 ---------------------SIDVFSFYVFGG------SGSSRFQPLSWSLRMKVALGA 180
                                 + V+ F   G         SSRFQPLSWSLRMKVALGA
Sbjct: 121 LGQLHHPNLVRLIGFCLEDEHRLLVYEFMPRGSLENHLFRRSSRFQPLSWSLRMKVALGA 180

Query: 181 AKGLAFLHSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFG 240
           AKGLAFLHSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFG
Sbjct: 181 AKGLAFLHSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFG 240

Query: 241 YAAPEYMITG-------------------------------------------------- 300
           YAAPEYMITG                                                  
Sbjct: 241 YAAPEYMITGHLTSKSDVYSFGVVLLEILSGRRAIDKNRPSGEHNLVEWAKPYLASKRRI 300

[Close]

BLAST of Cp4.1LG13g09430 vs. NCBI nr
Match: KAG7016231.1 (putative serine/threonine-protein kinase PBL9, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 498 bits (1283), Expect = 1.51e-173
Identity = 283/395 (71.65%), Postives = 287/395 (72.66%), Query Frame = 0


Query: 1   MVIYNASYTGLSSKDSRD--DKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKT 60
           MV+YNA YTGLSSKD+RD  DKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKT
Sbjct: 74  MVVYNAFYTGLSSKDARDGDDKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKT 133

Query: 61  ATRNFRPDSVLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLVSI 120
           ATRNFRPDSVLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWL  I
Sbjct: 134 ATRNFRPDSVLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLTEI 193

Query: 121 SL---------------------SIDVFSFYVFGG------SGSSRFQPLSWSLRMKVAL 180
                                   + V+ F   G         SSRFQPLSWSLRMKVAL
Sbjct: 194 DYLGQLHHPNLVRLIGFCLEDEHRLLVYEFMPRGSLENHLFRRSSRFQPLSWSLRMKVAL 253

Query: 181 GAAKGLAFLHSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGT 240
           GAAKGLAFLHSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGT
Sbjct: 254 GAAKGLAFLHSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGT 313

Query: 241 FGYAAPEYMITG------------------------------------------------ 300
           FGYAAPEYMITG                                                
Sbjct: 314 FGYAAPEYMITGHLTSKSDVYSFGVVLLEILSGRRAIDKNRPSGEHNLVEWAKPYLASKR 373

[Close]

BLAST of Cp4.1LG13g09430 vs. NCBI nr
Match: XP_023549887.1 (receptor-like cytoplasmic kinase 176 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 490 bits (1261), Expect = 3.22e-171
Identity = 277/386 (71.76%), Postives = 280/386 (72.54%), Query Frame = 0


Query: 8   YTGLSSKDSRDDKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRPDS 67
           + GLSSKDSRDDKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRPDS
Sbjct: 16  HNGLSSKDSRDDKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRPDS 75

Query: 68  VLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLVSISL------- 127
           VLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWL  I         
Sbjct: 76  VLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLTEIDYLGQLHHP 135

Query: 128 --------------SIDVFSFYVFGG------SGSSRFQPLSWSLRMKVALGAAKGLAFL 187
                          + V+ F   G         SSRFQPLSWSLRMKVALGAAKGLAFL
Sbjct: 136 NLVRLIGFCLEDEHRLLVYEFMPRGSLENHLFRRSSRFQPLSWSLRMKVALGAAKGLAFL 195

Query: 188 HSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAPEYM 247
           HSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAPEYM
Sbjct: 196 HSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAPEYM 255

Query: 248 ITG--------------------------------------------------------- 301
           ITG                                                         
Sbjct: 256 ITGHLTSKSDVYSFGVVLLEILSGRRAIDKNRPSGEHNLVEWAKPYLASKRRILRILDAR 315

[Close]

BLAST of Cp4.1LG13g09430 vs. NCBI nr
Match: XP_022992695.1 (receptor-like cytoplasmic kinase 176 [Cucurbita maxima])

HSP 1 Score: 486 bits (1251), Expect = 1.07e-169
Identity = 275/386 (71.24%), Postives = 279/386 (72.28%), Query Frame = 0


Query: 8   YTGLSSKDSRDDKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRPDS 67
           + GLSSKDSRDDKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRPDS
Sbjct: 16  HNGLSSKDSRDDKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRPDS 75

Query: 68  VLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLVSISL------- 127
           VLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWL  I         
Sbjct: 76  VLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLTEIDYLGQLHHP 135

Query: 128 --------------SIDVFSFYVFGG------SGSSRFQPLSWSLRMKVALGAAKGLAFL 187
                          + V+ F   G         SSRFQPLSWSLRMKVALGAAKGLAFL
Sbjct: 136 NLVRLIGFCLEDEHRLLVYEFMPRGSLENHLFRRSSRFQPLSWSLRMKVALGAAKGLAFL 195

Query: 188 HSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAPEYM 247
           HSDEAKVIYRDFKSSNILLDSSY+AKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAPEYM
Sbjct: 196 HSDEAKVIYRDFKSSNILLDSSYSAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAPEYM 255

Query: 248 ITG--------------------------------------------------------- 301
           ITG                                                         
Sbjct: 256 ITGHLTSKSDVYSFGVVLLEILSGRRAIDKNRPSGEHNLVEWAKPYLASKRRILRILDAR 315

[Close]

BLAST of Cp4.1LG13g09430 vs. NCBI nr
Match: XP_022939262.1 (receptor-like cytoplasmic kinase 176 [Cucurbita moschata])

HSP 1 Score: 484 bits (1245), Expect = 9.37e-169
Identity = 276/388 (71.13%), Postives = 280/388 (72.16%), Query Frame = 0


Query: 8   YTGLSSKDSRD--DKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRP 67
           + GLSSKD+RD  DKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRP
Sbjct: 16  HNGLSSKDARDGDDKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRP 75

Query: 68  DSVLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLVSISL----- 127
           DSVLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWL  I       
Sbjct: 76  DSVLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLTEIDYLGQLH 135

Query: 128 ----------------SIDVFSFYVFGG------SGSSRFQPLSWSLRMKVALGAAKGLA 187
                            + V+ F   G         SSRFQPLSWSLRMKVALGAAKGLA
Sbjct: 136 HPNLVRLIGFCLEDEHRLLVYEFMPRGSLENHLFRRSSRFQPLSWSLRMKVALGAAKGLA 195

Query: 188 FLHSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAPE 247
           FLHSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAPE
Sbjct: 196 FLHSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAPE 255

Query: 248 YMITG------------------------------------------------------- 301
           YMITG                                                       
Sbjct: 256 YMITGHLTSKSDVYSFGVVLLEILSGRRAIDKNRPSGEHNLVEWAKPYLASKRRILRILD 315

[Close]

BLAST of Cp4.1LG13g09430 vs. ExPASy TrEMBL
Match: A0A6J1JWF4 (receptor-like cytoplasmic kinase 176 OS=Cucurbita maxima OX=3661 GN=LOC111488958 PE=3 SV=1)

HSP 1 Score: 486 bits (1251), Expect = 5.17e-170
Identity = 275/386 (71.24%), Postives = 279/386 (72.28%), Query Frame = 0


Query: 8   YTGLSSKDSRDDKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRPDS 67
           + GLSSKDSRDDKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRPDS
Sbjct: 16  HNGLSSKDSRDDKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRPDS 75

Query: 68  VLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLVSISL------- 127
           VLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWL  I         
Sbjct: 76  VLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLTEIDYLGQLHHP 135

Query: 128 --------------SIDVFSFYVFGG------SGSSRFQPLSWSLRMKVALGAAKGLAFL 187
                          + V+ F   G         SSRFQPLSWSLRMKVALGAAKGLAFL
Sbjct: 136 NLVRLIGFCLEDEHRLLVYEFMPRGSLENHLFRRSSRFQPLSWSLRMKVALGAAKGLAFL 195

Query: 188 HSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAPEYM 247
           HSDEAKVIYRDFKSSNILLDSSY+AKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAPEYM
Sbjct: 196 HSDEAKVIYRDFKSSNILLDSSYSAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAPEYM 255

Query: 248 ITG--------------------------------------------------------- 301
           ITG                                                         
Sbjct: 256 ITGHLTSKSDVYSFGVVLLEILSGRRAIDKNRPSGEHNLVEWAKPYLASKRRILRILDAR 315

[Close]

BLAST of Cp4.1LG13g09430 vs. ExPASy TrEMBL
Match: A0A6J1FGB3 (receptor-like cytoplasmic kinase 176 OS=Cucurbita moschata OX=3662 GN=LOC111445231 PE=3 SV=1)

HSP 1 Score: 484 bits (1245), Expect = 4.54e-169
Identity = 276/388 (71.13%), Postives = 280/388 (72.16%), Query Frame = 0


Query: 8   YTGLSSKDSRD--DKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRP 67
           + GLSSKD+RD  DKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRP
Sbjct: 16  HNGLSSKDARDGDDKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRP 75

Query: 68  DSVLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLVSISL----- 127
           DSVLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWL  I       
Sbjct: 76  DSVLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLTEIDYLGQLH 135

Query: 128 ----------------SIDVFSFYVFGG------SGSSRFQPLSWSLRMKVALGAAKGLA 187
                            + V+ F   G         SSRFQPLSWSLRMKVALGAAKGLA
Sbjct: 136 HPNLVRLIGFCLEDEHRLLVYEFMPRGSLENHLFRRSSRFQPLSWSLRMKVALGAAKGLA 195

Query: 188 FLHSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAPE 247
           FLHSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAPE
Sbjct: 196 FLHSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAPE 255

Query: 248 YMITG------------------------------------------------------- 301
           YMITG                                                       
Sbjct: 256 YMITGHLTSKSDVYSFGVVLLEILSGRRAIDKNRPSGEHNLVEWAKPYLASKRRILRILD 315

[Close]

BLAST of Cp4.1LG13g09430 vs. ExPASy TrEMBL
Match: A0A6J1E1G2 (receptor-like cytoplasmic kinase 176 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429916 PE=3 SV=1)

HSP 1 Score: 451 bits (1161), Expect = 6.55e-156
Identity = 258/418 (61.72%), Postives = 279/418 (66.75%), Query Frame = 0


Query: 3   IYNASYTGLSSKDSRD--DKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTAT 62
           + N   TGLS K +RD  DKGNSISKSSSVSTPRTPRTEGEILQSSNLKNF YN+LKTAT
Sbjct: 12  LNNGFCTGLSFKSARDGNDKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFTYNDLKTAT 71

Query: 63  RNFRPDSVLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLVSI-- 122
           RNFRPDSVLGEGGFGSVFKGW+DEQSFAATKPGTGLVIAVKRLNQEGFQGHREWL  I  
Sbjct: 72  RNFRPDSVLGEGGFGSVFKGWVDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLTEIDY 131

Query: 123 --------------------------------SLSIDVF------------------SFY 182
                                           SL   +F                  SFY
Sbjct: 132 LGQLHHPNLVRLIGFCLEDEHRLLVYEFMPRGSLENHLFRSKLFTYSTCIPTCLLRFSFY 191

Query: 183 VFGGSGSSRFQPLSWSLRMKVALGAAKGLAFLHSDEAKVIYRDFKSSNILLDSSYNAKLS 242
           +FG SGS+ FQPLSWSLR+KVALGAAKGLAFLHS+EAKVIYRDFKSSNILLDS YNAKLS
Sbjct: 192 LFGASGSAHFQPLSWSLRLKVALGAAKGLAFLHSEEAKVIYRDFKSSNILLDSDYNAKLS 251

Query: 243 DFGLAKDGPTGDKSYVSTRVMGTFGYAAPEYMITG------------------------- 301
           DFGLAKDGPTGD+SYVSTRVMGT+GYAAPEYMITG                         
Sbjct: 252 DFGLAKDGPTGDRSYVSTRVMGTYGYAAPEYMITGHLTAKSDVYSFGVVLLEILSGKRAI 311

[Close]

BLAST of Cp4.1LG13g09430 vs. ExPASy TrEMBL
Match: A0A6J1E6R8 (receptor-like cytoplasmic kinase 176 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111429916 PE=3 SV=1)

HSP 1 Score: 449 bits (1156), Expect = 3.64e-155
Identity = 256/413 (61.99%), Postives = 277/413 (67.07%), Query Frame = 0


Query: 8   YTGLSSKDSRD--DKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRP 67
           + GLS K +RD  DKGNSISKSSSVSTPRTPRTEGEILQSSNLKNF YN+LKTATRNFRP
Sbjct: 16  HIGLSFKSARDGNDKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFTYNDLKTATRNFRP 75

Query: 68  DSVLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLVSI------- 127
           DSVLGEGGFGSVFKGW+DEQSFAATKPGTGLVIAVKRLNQEGFQGHREWL  I       
Sbjct: 76  DSVLGEGGFGSVFKGWVDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLTEIDYLGQLH 135

Query: 128 ---------------------------SLSIDVF------------------SFYVFGGS 187
                                      SL   +F                  SFY+FG S
Sbjct: 136 HPNLVRLIGFCLEDEHRLLVYEFMPRGSLENHLFRSKLFTYSTCIPTCLLRFSFYLFGAS 195

Query: 188 GSSRFQPLSWSLRMKVALGAAKGLAFLHSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLA 247
           GS+ FQPLSWSLR+KVALGAAKGLAFLHS+EAKVIYRDFKSSNILLDS YNAKLSDFGLA
Sbjct: 196 GSAHFQPLSWSLRLKVALGAAKGLAFLHSEEAKVIYRDFKSSNILLDSDYNAKLSDFGLA 255

Query: 248 KDGPTGDKSYVSTRVMGTFGYAAPEYMITG------------------------------ 301
           KDGPTGD+SYVSTRVMGT+GYAAPEYMITG                              
Sbjct: 256 KDGPTGDRSYVSTRVMGTYGYAAPEYMITGHLTAKSDVYSFGVVLLEILSGKRAIDKNRP 315

[Close]

BLAST of Cp4.1LG13g09430 vs. ExPASy TrEMBL
Match: A0A6J1JJX4 (receptor-like cytoplasmic kinase 176 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111487599 PE=3 SV=1)

HSP 1 Score: 449 bits (1156), Expect = 3.76e-155
Identity = 258/419 (61.58%), Postives = 278/419 (66.35%), Query Frame = 0


Query: 2   VIYNASYTGLSSKDSRD--DKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTA 61
            + N   TGLS K +RD  DKGNSISKSSSVSTPRTPRTEGEILQSSNLKNF YN+LKTA
Sbjct: 11  ALNNGFCTGLSFKSARDGNDKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFTYNDLKTA 70

Query: 62  TRNFRPDSVLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLVSI- 121
           TRNFRPDSVLGEGGFGSVFKGW+DEQSFAATKPGTGLVIAVKRLNQEGFQGHREWL  I 
Sbjct: 71  TRNFRPDSVLGEGGFGSVFKGWVDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLTEID 130

Query: 122 ---------------------------------SLSIDVF------------------SF 181
                                            SL   +F                  SF
Sbjct: 131 YLGQLHHPNLVRLIGFCLEDEHRLLVYEFMPRGSLENHLFRSKLFTYSTCIATCLLRFSF 190

Query: 182 YVFGGSGSSRFQPLSWSLRMKVALGAAKGLAFLHSDEAKVIYRDFKSSNILLDSSYNAKL 241
            +FG SGS+ FQPLSWSLR+KVALGAAKGLAFLHS+EAKVIYRDFKSSNILLDS YNAKL
Sbjct: 191 SLFGASGSAHFQPLSWSLRLKVALGAAKGLAFLHSEEAKVIYRDFKSSNILLDSDYNAKL 250

Query: 242 SDFGLAKDGPTGDKSYVSTRVMGTFGYAAPEYMITG------------------------ 301
           SDFGLAKDGPTGD+SYVSTRVMGT+GYAAPEYMITG                        
Sbjct: 251 SDFGLAKDGPTGDRSYVSTRVMGTYGYAAPEYMITGHLTAKSDVYSFGVVLLEILSGRRA 310

[Close]

BLAST of Cp4.1LG13g09430 vs. TAIR 10
Match: AT2G28930.1 (protein kinase 1B )

HSP 1 Score: 298.1 bits (762), Expect = 7.8e-81
Identity = 190/397 (47.86%), Postives = 222/397 (55.92%), Query Frame = 0


Query: 10  GLSSKDSRDDKGNSI-SKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRPDSV 69
           G S K    +  +S+ SKSSSVS    PRTEGEILQS NLK+F + ELK ATRNFRPDSV
Sbjct: 26  GASPKYMSSEANDSLGSKSSSVSIRTNPRTEGEILQSPNLKSFTFAELKAATRNFRPDSV 85

Query: 70  LGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLVSISLSIDVFS-- 129
           LGEGGFGSVFKGWIDEQ+  A+KPGTG+VIAVK+LNQ+G+QGH+EWL  ++  +  FS  
Sbjct: 86  LGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKKLNQDGWQGHQEWLAEVNY-LGQFSHP 145

Query: 130 -----------------FYVFGGSGS---------SRFQPLSWSLRMKVALGAAKGLAFL 189
                             Y F   GS         S FQPLSW+LR+KVALGAAKGLAFL
Sbjct: 146 NLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQPLSWTLRLKVALGAAKGLAFL 205

Query: 190 HSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAPEYM 249
           H+ E  VIYRDFK+SNILLDS YNAKLSDFGLAKDGPTGDKS+VSTR+MGT+GYAAPEY+
Sbjct: 206 HNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYL 265

Query: 250 ITG--------------------------------------------------------- 300
            TG                                                         
Sbjct: 266 ATGHLTTKSDVYSYGVVLLEVLSGRRAVDKNRPPGEQKLVEWARPLLANKRKLFRVIDNR 325

[Close]

BLAST of Cp4.1LG13g09430 vs. TAIR 10
Match: AT2G28930.2 (protein kinase 1B )

HSP 1 Score: 298.1 bits (762), Expect = 7.8e-81
Identity = 190/397 (47.86%), Postives = 222/397 (55.92%), Query Frame = 0


Query: 10  GLSSKDSRDDKGNSI-SKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRPDSV 69
           G S K    +  +S+ SKSSSVS    PRTEGEILQS NLK+F + ELK ATRNFRPDSV
Sbjct: 15  GASPKYMSSEANDSLGSKSSSVSIRTNPRTEGEILQSPNLKSFTFAELKAATRNFRPDSV 74

Query: 70  LGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLVSISLSIDVFS-- 129
           LGEGGFGSVFKGWIDEQ+  A+KPGTG+VIAVK+LNQ+G+QGH+EWL  ++  +  FS  
Sbjct: 75  LGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKKLNQDGWQGHQEWLAEVNY-LGQFSHP 134

Query: 130 -----------------FYVFGGSGS---------SRFQPLSWSLRMKVALGAAKGLAFL 189
                             Y F   GS         S FQPLSW+LR+KVALGAAKGLAFL
Sbjct: 135 NLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQPLSWTLRLKVALGAAKGLAFL 194

Query: 190 HSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAPEYM 249
           H+ E  VIYRDFK+SNILLDS YNAKLSDFGLAKDGPTGDKS+VSTR+MGT+GYAAPEY+
Sbjct: 195 HNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYL 254

Query: 250 ITG--------------------------------------------------------- 300
            TG                                                         
Sbjct: 255 ATGHLTTKSDVYSYGVVLLEVLSGRRAVDKNRPPGEQKLVEWARPLLANKRKLFRVIDNR 314

[Close]

BLAST of Cp4.1LG13g09430 vs. TAIR 10
Match: AT2G28930.3 (protein kinase 1B )

HSP 1 Score: 298.1 bits (762), Expect = 7.8e-81
Identity = 190/397 (47.86%), Postives = 222/397 (55.92%), Query Frame = 0


Query: 10  GLSSKDSRDDKGNSI-SKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRPDSV 69
           G S K    +  +S+ SKSSSVS    PRTEGEILQS NLK+F + ELK ATRNFRPDSV
Sbjct: 18  GASPKYMSSEANDSLGSKSSSVSIRTNPRTEGEILQSPNLKSFTFAELKAATRNFRPDSV 77

Query: 70  LGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLVSISLSIDVFS-- 129
           LGEGGFGSVFKGWIDEQ+  A+KPGTG+VIAVK+LNQ+G+QGH+EWL  ++  +  FS  
Sbjct: 78  LGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKKLNQDGWQGHQEWLAEVNY-LGQFSHP 137

Query: 130 -----------------FYVFGGSGS---------SRFQPLSWSLRMKVALGAAKGLAFL 189
                             Y F   GS         S FQPLSW+LR+KVALGAAKGLAFL
Sbjct: 138 NLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQPLSWTLRLKVALGAAKGLAFL 197

Query: 190 HSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAPEYM 249
           H+ E  VIYRDFK+SNILLDS YNAKLSDFGLAKDGPTGDKS+VSTR+MGT+GYAAPEY+
Sbjct: 198 HNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYL 257

Query: 250 ITG--------------------------------------------------------- 300
            TG                                                         
Sbjct: 258 ATGHLTTKSDVYSYGVVLLEVLSGRRAVDKNRPPGEQKLVEWARPLLANKRKLFRVIDNR 317

[Close]

BLAST of Cp4.1LG13g09430 vs. TAIR 10
Match: AT1G07570.2 (Protein kinase superfamily protein )

HSP 1 Score: 297.7 bits (761), Expect = 1.0e-80
Identity = 188/399 (47.12%), Postives = 223/399 (55.89%), Query Frame = 0


Query: 6   ASYTGLSSKDSRDDKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRP 65
           A  +G S+K    D G+  SK+SSVS   +PRTEGEILQS NLK+F + ELK+ATRNFRP
Sbjct: 11  AESSGASTKYDAKDIGSLGSKASSVSVRPSPRTEGEILQSPNLKSFSFAELKSATRNFRP 70

Query: 66  DSVLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLVSISLSIDVF 125
           DSVLGEGGFG VFKGWIDE+S  A++PGTGLVIAVK+LNQ+G+QGH+EWL  ++  +  F
Sbjct: 71  DSVLGEGGFGCVFKGWIDEKSLTASRPGTGLVIAVKKLNQDGWQGHQEWLAEVNY-LGQF 130

Query: 126 S-------------------FYVFGGSGSSR---------FQPLSWSLRMKVALGAAKGL 185
           S                    Y F   GS           FQPLSW LR+KVALGAAKGL
Sbjct: 131 SHRHLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGLYFQPLSWKLRLKVALGAAKGL 190

Query: 186 AFLHSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAP 245
           AFLHS E +VIYRDFK+SNILLDS YNAKLSDFGLAKDGP GDKS+VSTRVMGT GYAAP
Sbjct: 191 AFLHSSETRVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRVMGTHGYAAP 250

Query: 246 EYMITG------------------------------------------------------ 300
           EY+ TG                                                      
Sbjct: 251 EYLATGHLTTKSDVYSFGVVLLELLSGRRAVDKNRPSGERNLVEWAKPYLVNKRKIFRVI 310

[Close]

BLAST of Cp4.1LG13g09430 vs. TAIR 10
Match: AT1G07570.1 (Protein kinase superfamily protein )

HSP 1 Score: 297.7 bits (761), Expect = 1.0e-80
Identity = 188/399 (47.12%), Postives = 223/399 (55.89%), Query Frame = 0


Query: 6   ASYTGLSSKDSRDDKGNSISKSSSVSTPRTPRTEGEILQSSNLKNFPYNELKTATRNFRP 65
           A  +G S+K    D G+  SK+SSVS   +PRTEGEILQS NLK+F + ELK+ATRNFRP
Sbjct: 11  AESSGASTKYDAKDIGSLGSKASSVSVRPSPRTEGEILQSPNLKSFSFAELKSATRNFRP 70

Query: 66  DSVLGEGGFGSVFKGWIDEQSFAATKPGTGLVIAVKRLNQEGFQGHREWLVSISLSIDVF 125
           DSVLGEGGFG VFKGWIDE+S  A++PGTGLVIAVK+LNQ+G+QGH+EWL  ++  +  F
Sbjct: 71  DSVLGEGGFGCVFKGWIDEKSLTASRPGTGLVIAVKKLNQDGWQGHQEWLAEVNY-LGQF 130

Query: 126 S-------------------FYVFGGSGSSR---------FQPLSWSLRMKVALGAAKGL 185
           S                    Y F   GS           FQPLSW LR+KVALGAAKGL
Sbjct: 131 SHRHLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGLYFQPLSWKLRLKVALGAAKGL 190

Query: 186 AFLHSDEAKVIYRDFKSSNILLDSSYNAKLSDFGLAKDGPTGDKSYVSTRVMGTFGYAAP 245
           AFLHS E +VIYRDFK+SNILLDS YNAKLSDFGLAKDGP GDKS+VSTRVMGT GYAAP
Sbjct: 191 AFLHSSETRVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRVMGTHGYAAP 250

Query: 246 EYMITG------------------------------------------------------ 300
           EY+ TG                                                      
Sbjct: 251 EYLATGHLTTKSDVYSFGVVLLELLSGRRAVDKNRPSGERNLVEWAKPYLVNKRKIFRVI 310

The following BLAST results are available for this feature:

BLAST of Cp4.1LG13g09430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q65XV8	4.0e-82	48.94	Receptor-like cytoplasmic kinase 176 OS=Oryza sativa subsp. japonica OX=39947 GN...	[more]
P46573	1.1e-79	47.86	Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q06548	1.4e-79	47.12	Probable serine/threonine-protein kinase PBL9 OS=Arabidopsis thaliana OX=3702 GN...	[more]
P43293	9.0e-74	46.61	Probable serine/threonine-protein kinase PBL11 OS=Arabidopsis thaliana OX=3702 G...	[more]
O48814	1.9e-71	47.54	Serine/threonine-protein kinase BIK1 OS=Arabidopsis thaliana OX=3702 GN=BIK1 PE=...	[more]

BLAST of Cp4.1LG13g09430 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023549888.1	3.46e-178	72.77	receptor-like cytoplasmic kinase 176 isoform X2 [Cucurbita pepo subsp. pepo]	[more]
KAG7016231.1	1.51e-173	71.65	putative serine/threonine-protein kinase PBL9, partial [Cucurbita argyrosperma s...	[more]
XP_023549887.1	3.22e-171	71.76	receptor-like cytoplasmic kinase 176 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022992695.1	1.07e-169	71.24	receptor-like cytoplasmic kinase 176 [Cucurbita maxima]	[more]
XP_022939262.1	9.37e-169	71.13	receptor-like cytoplasmic kinase 176 [Cucurbita moschata]	[more]

BLAST of Cp4.1LG13g09430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JWF4	5.17e-170	71.24	receptor-like cytoplasmic kinase 176 OS=Cucurbita maxima OX=3661 GN=LOC111488958...	[more]
A0A6J1FGB3	4.54e-169	71.13	receptor-like cytoplasmic kinase 176 OS=Cucurbita moschata OX=3662 GN=LOC1114452...	[more]
A0A6J1E1G2	6.55e-156	61.72	receptor-like cytoplasmic kinase 176 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1E6R8	3.64e-155	61.99	receptor-like cytoplasmic kinase 176 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1JJX4	3.76e-155	61.58	receptor-like cytoplasmic kinase 176 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]

BLAST of Cp4.1LG13g09430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28930.1	7.8e-81	47.86	protein kinase 1B	[more]
AT2G28930.2	7.8e-81	47.86	protein kinase 1B	[more]
AT2G28930.3	7.8e-81	47.86	protein kinase 1B	[more]
AT1G07570.2	1.0e-80	47.12	Protein kinase superfamily protein	[more]
AT1G07570.1	1.0e-80	47.12	Protein kinase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 133..229 e-value: 2.5E-27 score: 97.4
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 34..124 e-value: 5.2E-15 score: 57.4
None	No IPR available	PIRSR	PIRSR000620-2	PIRSR000620-2	coord: 147..200 e-value: 0.0037 score: 14.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 259..301
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..43
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 23..43
None	No IPR available	PANTHER	PTHR45621:SF184	KINASE 1B	coord: 135..223 coord: 225..287
None	No IPR available	PANTHER	PTHR45621:SF184	KINASE 1B	coord: 18..119
None	No IPR available	PANTHER	PTHR45621	OS01G0588500 PROTEIN-RELATED	coord: 135..223
None	No IPR available	PANTHER	PTHR45621	OS01G0588500 PROTEIN-RELATED	coord: 18..119 coord: 225..287
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 148..220 e-value: 1.4E-15 score: 57.4
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 63..301 score: 17.276709
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 167..179
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 69..101
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 39..255

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG13g09430.1	Cp4.1LG13g09430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity