Cp4.1LG13g08620 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG13g08620
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Translation factor GUF1 homolog, mitochondrial
Location	Cp4.1LG13: 7927405 .. 7931359 (-)
RNA-Seq Expression	Cp4.1LG13g08620
Synteny	Cp4.1LG13g08620

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAAACGTCGAAGAAGTTGGAATCGATTTAACGCAGTTCCCTCCCGATCGGATTAGAAACTTTTCTATAATCGCTCATGTGGATCACGGCAAGTATACATTGGCTGATCGACTTTTGGAGCTCCCTGGTACTATTAAGCGAGGCCATGGTCGGCCTCTATACCTCGATAAGTTGCAGGTTCGTTGGCCATGCTTAGCGACTTTTATTGGAATATGATTACTTTCTTACACTTTCGTATGGTCGCCAACTTTTGTCGAAGAAATTGTGTTTGTGTAAGTCTATTGTCAAATTAACTAGGAAAACTAAGTTTGCCAAAGTCGTTATTTGCTAATTTTCTTCATGATTCCGCTGTATCTGTTGAGAAATTAGGTATGCATTGCTTGAAGGTTTTCCTGTTGATCTTGTTCTATTATCGAAGTCGTTGTTTAACTCAGCATGGGATCTTTTTTTCTTTTCTTAGGTGGAAAATTTACATTCTCTAGTTATTCTGTCATCTTATGCTATAAAACTGATTTCCTTAATGGTTTTTCATTTTTTTTAAAAAAAGCTTGATGAAGCCCAATTATTTAGGTGGAGAGGGAGAGGGGAATTACTGTTAAAGCTCAGACTGCAACCATGTATCACAAGTATAATCTTGTTGGTGACAACACTAGCGAACGCCTGGCCATGTTGATTTCAGCTATGAAGTGTCGAGATCCTTAGCAGCTTGTCAGGGTCCTCTTTTGATTGTGGATGCGGCCCAAGGTGTTCAAGCCCAAACAGTGGCTAATTTTTATCTTGCTTTTGAATCTAATTTAGCAATTTTACCGGTTATTAACAAAATTGACCAGCCAACTGCTGATCCTGATCGTGTTAAAGCTCAACTGAAATCAATGTTCTGCACATGCTATGTCGACATCTGCCAAAACAGGGCAAGGTCTTGAGCATGTCCTTCCTGCCATTATAGAGCACATACCTCCACCTCCTGGGAAGAGCTCTTTACCTTTGCGAATGCTTTTATTAGATTTGTACTATGATGAATACAAGGGAGTGATATGCCATGTTGCTGTTGTTGGTGGTGTGCTGCGCAAGGGGGATAAGATTTCTTCTGTAGGCACTGGTCATGCTTTTGAAGTCTTGGATGTTGGGATCATGCATCCCGAACTTACCAGTACAGGGATCCTTCTTACCAGTCAAGTGGGTTATGTTGTGAGTGGAATGCGTTCTACCAAAGAGGCAAGAATTGGGGATACACTTCATCAGAGTAAAAGTGTTGTTGAACCCCTTCCCTGTAAATCCTTCATTTTCTTTTGCTACTTTGGTGTCTGCAATGGCTTGTCTGTTTCGTTTGCTACCTCTTAGTGACATTTGTCCTTCTTGTAGGTTTTAAGCCTGTTAAGCACAGGGTTTTCTCTGGTCTATATCCAGCTGACGGGTCAGATTTTGATGCACTCAACCATGCAATAGAGAGACTAACATGTAATGATGCCAGCGTCTCTGTAACTAAGGAGACTAGTACAGCATTAGGGCTTGATTTTAGGTACGGCTCCCACTGGCTGAATTGTTTCCTCTTGCTACGAAAATGGATTTATATTAGCTTAATTTTTCATGTATGTGAACCCATAATAATTGAGTCCTCTACGCAGGTGTGGTTCCTTTTCGTCTCCATTGACAGGTTTTTTTTTTTTTCCTTAAAGCTTAGAAGTTAGTACCCTCGAGTGCCTCTTGTTTGTTAGTGGCAATGTGTTAATATTCATGAATTCTTGTATTTTACCAAGAACATAATATTTTGTTTTTTTGTTTTCTTTTTTTTGTTTTTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNTTTATCCTGCAGTCAAAGGCATTTATGAATTATCGCGTACCTTTAAGGGAATTATTGTTGACTTTTACAATGAATTAAAGAGTATAACATCAGGATATGCATCATTCGATTACGAGGATTCAGAGTAACTCTATTGCCTTCTCTATTTCCATTTATAAACCTTAGCTATCGAATCTCCTTTAGTCTTTTTCTGCATGTTCTTAATGAAAGATGGTTAATTTTTTCATATTGTTTTCTTATTCAACATTGAATCATGGTCAATGTCTCTAGCTAATATGTATTCTCAGTTCTGTGGAAAACCTGATGTATATGTAGATTGTGAACAATTATGATTATTAGTATTATAGGCCCTTCTTAGATGGCTGTGCTGATATGATGAGTTTTTCAAGGATAGGTAATATGATTACATCATCCATATATTGATGTATTGATGATGGGTGCGATTACCAGGTATCAACAAGCAGATTTGGTGAAACTAGATAGCCTATTGAATGGGCAGCCAGTTTTCATAATTTGAAGGCACAAAGGGTCGGACGCGAATTGGTGGAAAAACTGAAGAAGTTCATTGACAGGCAGCAAATGTTTGAGATTACAATACAAGCTGCGATTGGATCTAATATTATTGCAAGAGAAACGTAATGTTCTTATTTTATTTACTTATTTTCTCTAGAAGGTTTTTACTCGGATTTTTATTACATATTCAATATTTGAGTGGCCCAAAACCTTGTAGTCGAACTCTTTCGCCCTGACACGAAATTATAGTTACTCATTGATTGCCTTCAATTTTCATTGTTAGACTGAATTATTTATGTTGATTTTAAGATCTCGGCCACGAGGAAAAATGTTCTTGCAAAGTGTTATGGTGGAGATGTTACTCGGAAGAGGAAGCTGTTGGAGAAACAAAAGGAAGGGAAAAAATGTATGAACGTGTTGGATCTGTTGATATTCCTCAGGAAGCAATTCATGAAATTCTGAAGGTTTCATAGGTGGAAAAATTCAGAGTAAATTTATGAACCATACTTATTACAGGTACGGTTCTTTTTTATCCATATGAATCACGTTTGGATTTCATTTCCACTTTGATGTTAATGATAATCGTGACATAAGTTTACAATTTTCAAATGGAAGTTTATTAAATAATATTTCAGTAAGTGAGGAGTATACTACATGTAGTTTTAGAGACCTCCAATTCTGCTGTCACAGTTTTGCCTAATCATATTGGTTTGCAACTCCGAATGATCTGTCCTGGCAGCTCATACTCAATCATCCCGTCATATCCCAAGATGCCAAGGTCGGGAGATTGTTAGTAGAATAGATTAGTCTAAGTTAGAGACATCTTTTTGGGTGGTGCAGTTAATCATTATTTATTTGTCATTTCCCTTGTATGTATTGTATGCGTAGCTGATCGATACAATATCAGATAAATTGAGATATCTTGATTTTAATGTGAGCCGTGTGACATTTACTGATTAGTTGCCATTATTAAACCTTTGATTTTGTGCATGTATTTGTGTGTTGGCTTTTGCTCGTGAGTTGTTATAGGCTATTGCTTCTGTAGAGGATCGTGAAAGCTTGAAGGGTGTTTCTTTTTCTTCAACCTTTTTTAATACTTGTTTGGGAAGGGTCTGATCAGTCGCCAGTCAATGTAGGAGCCTTATTTAATTCTCTCCCTATTCCTACTACCAAAGTAATTACTCAATATCATTAATATAGCTTTCTTTCATCATTCCTCCCATGTGAATAATCATTTAGTATGATTAACATAATTTTAACTCAAGCTTTGATATCATGAGTTTATAAAAGAGAGATTCAAAAATATTTCGTAGATTTGTATCTAATGATGACCAAAATGATAACCACTTCGAAACATCATTTATTACAGCAAGAGTTTTCTCAAATGGCCAGATACCCAGAAGATATATTCCACCGGGGTCTTGATCGATGATAGATAAATGTGGGATCTTCAACATGCTCCCTCAAGATCGTGTAAGCCTCTTCTTCTTTTTTATGTACGATAATCAACAAAAGTTCACATTTAATAATTTTTTTTTGCAAGATATCTGTTGATTTCCAATACTTTGTCAAAATAGGCAG

mRNA sequence

CAAAACGTCGAAGAAGTTGGAATCGATTTAACGCAGTTCCCTCCCGATCGGATTAGAAACTTTTCTATAATCGCTCATGTGGATCACGGCAAGTATACATTGGCTGATCGACTTTTGGAGCTCCCTGGTACTATTAAGCGAGGCCATGGTCGGCCTCTATACCTCGATAAGTTGCAGCGAACGCCTGGCCATGTTGATTTCAGCTATGAAGTGTCGAGATCCTTAGCAGCTTGTCAGGGTCCTCTTTTGATTGTGGATGCGGCCCAAGGGCAAGGTCTTGAGCATGTCCTTCCTGCCATTATAGAGCACATACCTCCACCTCCTGGGAAGAGCTCTTTACCTTTGCGAATGCTTTTATTAGATTTGTACTATGATGAATACAAGGGAGTGATATGCCATGTTGCTGTTGTTGGTGGTGTGCTGCGCAAGGGGGATAAGATTTCTTCTGTAGGCACTGGTCATGCTTTTGAAGTCTTGGATGTTGGGATCATGCATCCCGAACTTACCAGTACAGGGATCCTTCTTACCAGTCAAGTGGGTTATGTTGTGAGTGGAATGCGTTCTACCAAAGAGGCAAGAATTGGGGATACACTTCATCAGAGTAAAAGTGTTGTTGAACCCCTTCCCTTGACATTTGTCCTTCTTGTAGGTTTTAAGCCTGTTAAGCACAGGGTTTTCTCTGGTCTATATCCAGCTGACGGGTCAGATTTTGATGCACTCAACCATGCAATAGAGAGACTAACATGTAATGATGCCAGCGTCTCTGTAACTAAGGAGACTAGTACAGCATTAGGGCTTGATTTTAGGTACGGCTCCCACTGGCTGAATTGTTTCCTCTTGCTACGAAAATGGATTTATATTAGCTTAATTTTTCATATCTCGGCCACGAGGAAAAATGTTCTTGCAAAGTGTTATGGTGGAGATGTTACTCGGAAGAGGAAGCTGTTGGAGAAACAAAAGGAAGGGAAAAAATGTATGAACGTGTTGGATCTGTTGATATTCCTCAGGAAGCAATTCATGAAATTCTGAAGGTTTCATAGGTGGAAAAATTCAGAGTAAATTTATGAACCATACTTATTACAGCAAGAGTTTTCTCAAATGGCCAGATACCCAGAAGATATATTCCACCGGGGTCTTGATCGATGATAGATAAATGTGGGATCTTCAACATGCTCCCTCAAGATCGTGTAAGCCTCTTCTTCTTTTTTATGTACGATAATCAACAAAAGTTCACATTTAATAATTTTTTTTTGCAAGATATCTGTTGATTTCCAATACTTTGTCAAAATAGGCAG

Coding sequence (CDS)

CAAAACGTCGAAGAAGTTGGAATCGATTTAACGCAGTTCCCTCCCGATCGGATTAGAAACTTTTCTATAATCGCTCATGTGGATCACGGCAAGTATACATTGGCTGATCGACTTTTGGAGCTCCCTGGTACTATTAAGCGAGGCCATGGTCGGCCTCTATACCTCGATAAGTTGCAGCGAACGCCTGGCCATGTTGATTTCAGCTATGAAGTGTCGAGATCCTTAGCAGCTTGTCAGGGTCCTCTTTTGATTGTGGATGCGGCCCAAGGGCAAGGTCTTGAGCATGTCCTTCCTGCCATTATAGAGCACATACCTCCACCTCCTGGGAAGAGCTCTTTACCTTTGCGAATGCTTTTATTAGATTTGTACTATGATGAATACAAGGGAGTGATATGCCATGTTGCTGTTGTTGGTGGTGTGCTGCGCAAGGGGGATAAGATTTCTTCTGTAGGCACTGGTCATGCTTTTGAAGTCTTGGATGTTGGGATCATGCATCCCGAACTTACCAGTACAGGGATCCTTCTTACCAGTCAAGTGGGTTATGTTGTGAGTGGAATGCGTTCTACCAAAGAGGCAAGAATTGGGGATACACTTCATCAGAGTAAAAGTGTTGTTGAACCCCTTCCCTTGACATTTGTCCTTCTTGTAGGTTTTAAGCCTGTTAAGCACAGGGTTTTCTCTGGTCTATATCCAGCTGACGGGTCAGATTTTGATGCACTCAACCATGCAATAGAGAGACTAACATGTAATGATGCCAGCGTCTCTGTAACTAAGGAGACTAGTACAGCATTAGGGCTTGATTTTAGGTACGGCTCCCACTGGCTGAATTGTTTCCTCTTGCTACGAAAATGGATTTATATTAGCTTAATTTTTCATATCTCGGCCACGAGGAAAAATGTTCTTGCAAAGTGTTATGGTGGAGATGTTACTCGGAAGAGGAAGCTGTTGGAGAAACAAAAGGAAGGGAAAAAATGTATGAACGTGTTGGATCTGTTGATATTCCTCAGGAAGCAATTCATGAAATTCTGA

Protein sequence

QNVEEVGIDLTQFPPDRIRNFSIIAHVDHGKYTLADRLLELPGTIKRGHGRPLYLDKLQRTPGHVDFSYEVSRSLAACQGPLLIVDAAQGQGLEHVLPAIIEHIPPPPGKSSLPLRMLLLDLYYDEYKGVICHVAVVGGVLRKGDKISSVGTGHAFEVLDVGIMHPELTSTGILLTSQVGYVVSGMRSTKEARIGDTLHQSKSVVEPLPLTFVLLVGFKPVKHRVFSGLYPADGSDFDALNHAIERLTCNDASVSVTKETSTALGLDFRYGSHWLNCFLLLRKWIYISLIFHISATRKNVLAKCYGGDVTRKRKLLEKQKEGKKCMNVLDLLIFLRKQFMKF

Homology

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BLAST of Cp4.1LG13g08620 vs. ExPASy Swiss-Prot
Match: B9RUN8 (Translation factor GUF1 homolog, mitochondrial OS=Ricinus communis OX=3988 GN=RCOM_0855130 PE=3 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 1.9e-107
Identity = 215/364 (59.07%), Postives = 233/364 (64.01%), Query Frame = 0


Query: 8   IDLTQFPPDRIRNFSIIAHVDHGKYTLADRLLELPGTIKRGHGRPLYLDKLQ-------- 67
           IDL+++P +RIRNFSIIAHVDHGK TLADRLLEL GTIKRGHG+P YLDKLQ        
Sbjct: 60  IDLSKYPTERIRNFSIIAHVDHGKSTLADRLLELTGTIKRGHGQPQYLDKLQVERERGIT 119

Query: 68  ---------------------------------RTPGHVDFSYEVSRSLAACQGPLLIVD 127
                                             TPGHVDFSYEVSRSLAACQG LL+VD
Sbjct: 120 VKAQTATMFHKYNFHGPNIGDAHEPPTFLLNLIDTPGHVDFSYEVSRSLAACQGALLVVD 179

Query: 128 AAQ--------------------------------------------------------- 187
           AAQ                                                         
Sbjct: 180 AAQGVQAQTVANFYLAFESNLTVIPVINKIDQPTADPDRVKAQLKSMFDLEPSDCLLTSA 239

Query: 188 --GQGLEHVLPAIIEHIPPPPGKSSLPLRMLLLDLYYDEYKGVICHVAVVGGVLRKGDKI 247
             GQGLE VLPA+IE IP PPG S+ PLRMLLLD YYDEYKGVICHVAVV G+LRKGDKI
Sbjct: 240 KTGQGLEQVLPAVIERIPSPPGYSNSPLRMLLLDSYYDEYKGVICHVAVVDGMLRKGDKI 299

Query: 248 SSVGTGHAFEVLDVGIMHPELTSTGILLTSQVGYVVSGMRSTKEARIGDTLHQSKSVVEP 272
           SS  TGH++E+LDVG MHPELT TGILLT QVGYVVSGMRSTKEAR+GDTL+ S++ VEP
Sbjct: 300 SSAATGHSYEILDVGFMHPELTPTGILLTGQVGYVVSGMRSTKEARVGDTLYHSRTSVEP 359

HSP 2 Score: 65.1 bits (157), Expect = 1.8e-09
Identity = 32/34 (94.12%), Postives = 32/34 (94.12%), Query Frame = 0


Query: 293 ISATRKNVLAKCYGGDVTRKRKLLEKQKEGKKCM 327
           ISA RKNVLAKCYGGDVTRKRKLLEKQKEGKK M
Sbjct: 620 ISAMRKNVLAKCYGGDVTRKRKLLEKQKEGKKRM 653

[Close]

BLAST of Cp4.1LG13g08620 vs. ExPASy Swiss-Prot
Match: B8B2R1 (Translation factor GUF1 homolog, mitochondrial OS=Oryza sativa subsp. indica OX=39946 GN=OsI_21607 PE=3 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 6.0e-106
Identity = 209/366 (57.10%), Postives = 230/366 (62.84%), Query Frame = 0


Query: 6   VGIDLTQFPPDRIRNFSIIAHVDHGKYTLADRLLELPGTIKRGHGRPLYLDKLQ------ 65
           +G +L ++PP+R+RNFSIIAHVDHGK TLADRLLEL GTIK+GHG+P YLDKLQ      
Sbjct: 45  LGSELGRYPPERVRNFSIIAHVDHGKSTLADRLLELTGTIKKGHGQPQYLDKLQVERERG 104

Query: 66  -----------------------------------RTPGHVDFSYEVSRSLAACQGPLLI 125
                                               TPGHVDFSYEVSRSLAACQG LL+
Sbjct: 105 ITVKAQTATMFYRHANNQLPASDQPDAPSYLLNLIDTPGHVDFSYEVSRSLAACQGALLV 164

Query: 126 VDAAQ------------------------------------------------------- 185
           VDAAQ                                                       
Sbjct: 165 VDAAQGVQAQTIANFYLAFESNLSIIPVINKIDQPTADPDNVKAQLKRLFDIDPSEALLT 224

Query: 186 ----GQGLEHVLPAIIEHIPPPPGKSSLPLRMLLLDLYYDEYKGVICHVAVVGGVLRKGD 245
               GQGL  VLPA+IE IP PPGK   P+RMLLLD YYDEYKGVICHVAVV G L KGD
Sbjct: 225 SAKTGQGLSQVLPAVIERIPSPPGKCDSPVRMLLLDSYYDEYKGVICHVAVVDGALHKGD 284

Query: 246 KISSVGTGHAFEVLDVGIMHPELTSTGILLTSQVGYVVSGMRSTKEARIGDTLHQSKSVV 272
           KI+S  TG  +EVLDVGIMHPELT TG+L T QVGYV+SGMRSTKEARIGDTLHQ+KS+V
Sbjct: 285 KIASAATGRTYEVLDVGIMHPELTPTGVLYTGQVGYVISGMRSTKEARIGDTLHQAKSIV 344

HSP 2 Score: 62.8 bits (151), Expect = 8.8e-09
Identity = 29/34 (85.29%), Postives = 32/34 (94.12%), Query Frame = 0


Query: 293 ISATRKNVLAKCYGGDVTRKRKLLEKQKEGKKCM 327
           +SA RKNVLAKCYGGD+TRK+KLLEKQKEGKK M
Sbjct: 592 LSAMRKNVLAKCYGGDITRKKKLLEKQKEGKKRM 625

[Close]

BLAST of Cp4.1LG13g08620 vs. ExPASy Swiss-Prot
Match: Q5VQ69 (Translation factor GUF1 homolog, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0144800 PE=3 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 1.3e-105
Identity = 209/366 (57.10%), Postives = 229/366 (62.57%), Query Frame = 0


Query: 6   VGIDLTQFPPDRIRNFSIIAHVDHGKYTLADRLLELPGTIKRGHGRPLYLDKLQ------ 65
           +G +L  +PP+R+RNFSIIAHVDHGK TLADRLLEL GTIK+GHG+P YLDKLQ      
Sbjct: 45  LGSELGLYPPERVRNFSIIAHVDHGKSTLADRLLELTGTIKKGHGQPQYLDKLQVERERG 104

Query: 66  -----------------------------------RTPGHVDFSYEVSRSLAACQGPLLI 125
                                               TPGHVDFSYEVSRSLAACQG LL+
Sbjct: 105 ITVKAQTATMFYRHANNQLPASDQPDAPSYLLNLIDTPGHVDFSYEVSRSLAACQGALLV 164

Query: 126 VDAAQ------------------------------------------------------- 185
           VDAAQ                                                       
Sbjct: 165 VDAAQGVQAQTIANFYLAFESNLSIIPVINKIDQPTADPDNVKAQLKRLFDIDPSEALLT 224

Query: 186 ----GQGLEHVLPAIIEHIPPPPGKSSLPLRMLLLDLYYDEYKGVICHVAVVGGVLRKGD 245
               GQGL  VLPA+IE IP PPGK   P+RMLLLD YYDEYKGVICHVAVV G L KGD
Sbjct: 225 SAKTGQGLSQVLPAVIERIPSPPGKCDSPVRMLLLDSYYDEYKGVICHVAVVDGALHKGD 284

Query: 246 KISSVGTGHAFEVLDVGIMHPELTSTGILLTSQVGYVVSGMRSTKEARIGDTLHQSKSVV 272
           KI+S  TG  +EVLDVGIMHPELT TG+L T QVGYV+SGMRSTKEARIGDTLHQ+KS+V
Sbjct: 285 KIASAATGRTYEVLDVGIMHPELTPTGVLYTGQVGYVISGMRSTKEARIGDTLHQAKSIV 344

HSP 2 Score: 62.8 bits (151), Expect = 8.8e-09
Identity = 29/34 (85.29%), Postives = 32/34 (94.12%), Query Frame = 0


Query: 293 ISATRKNVLAKCYGGDVTRKRKLLEKQKEGKKCM 327
           +SA RKNVLAKCYGGD+TRK+KLLEKQKEGKK M
Sbjct: 607 LSAMRKNVLAKCYGGDITRKKKLLEKQKEGKKRM 640

[Close]

BLAST of Cp4.1LG13g08620 vs. ExPASy Swiss-Prot
Match: C5Z3W1 (Translation factor GUF1 homolog, mitochondrial OS=Sorghum bicolor OX=4558 GN=Sb10g003070 PE=3 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 1.7e-105
Identity = 207/361 (57.34%), Postives = 228/361 (63.16%), Query Frame = 0


Query: 7   GIDLTQFPPDRIRNFSIIAHVDHGKYTLADRLLELPGTIKRGHGRPLYLDKLQ------- 66
           G +L  +PP+R+RNFSIIAHVDHGK TLADRLLEL GTI++GHG+P YLDKLQ       
Sbjct: 52  GSELALYPPERVRNFSIIAHVDHGKSTLADRLLELTGTIQKGHGQPQYLDKLQVERERGI 111

Query: 67  ------------------------------RTPGHVDFSYEVSRSLAACQGPLLIVDAAQ 126
                                          TPGHVDFSYEVSRSLAACQG LL+VDAAQ
Sbjct: 112 TVKAQTATMFYRHVSASQDSDTPKYLLNLIDTPGHVDFSYEVSRSLAACQGALLVVDAAQ 171

Query: 127 -----------------------------------------------------------G 186
                                                                      G
Sbjct: 172 GVQAQTIANFYLAFESNLSIIPVINKIDQPTADPDNVKDQLKRLFDIDPSEALLTSAKTG 231

Query: 187 QGLEHVLPAIIEHIPPPPGKSSLPLRMLLLDLYYDEYKGVICHVAVVGGVLRKGDKISSV 246
           QGLE VLPA+IE IP PPGK   P+RMLLLD YYDEYKGVICHVA+V G LRKGDKI+S 
Sbjct: 232 QGLEQVLPAVIERIPSPPGKCDAPVRMLLLDSYYDEYKGVICHVAIVDGALRKGDKIASA 291

Query: 247 GTGHAFEVLDVGIMHPELTSTGILLTSQVGYVVSGMRSTKEARIGDTLHQSKSVVEPLPL 272
            TG A+EVLDVGIMHPEL  TG+L T QVGYV+SGMRSTKEARIGDTLHQ+KS VEPLP 
Sbjct: 292 ATGRAYEVLDVGIMHPELKPTGVLYTGQVGYVISGMRSTKEARIGDTLHQAKSTVEPLP- 351

HSP 2 Score: 62.8 bits (151), Expect = 8.8e-09
Identity = 29/34 (85.29%), Postives = 32/34 (94.12%), Query Frame = 0


Query: 293 ISATRKNVLAKCYGGDVTRKRKLLEKQKEGKKCM 327
           +SA RKNVLAKCYGGD+TRK+KLLEKQKEGKK M
Sbjct: 609 LSAMRKNVLAKCYGGDITRKKKLLEKQKEGKKRM 642

[Close]

BLAST of Cp4.1LG13g08620 vs. ExPASy Swiss-Prot
Match: Q9FLE4 (Translation factor GUF1 homolog, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g39900 PE=2 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 1.5e-101
Identity = 198/365 (54.25%), Postives = 235/365 (64.38%), Query Frame = 0


Query: 1   QNVEEVGIDLTQFPPDRIRNFSIIAHVDHGKYTLADRLLELPGTIKRGHGRPLYLDKLQ- 60
           Q+ +E  IDLT+FP ++IRNFSIIAH+DHGK TLADRL+EL GTIK+GHG+P YLDKLQ 
Sbjct: 49  QSSKEPTIDLTKFPSEKIRNFSIIAHIDHGKSTLADRLMELTGTIKKGHGQPQYLDKLQV 108

Query: 61  ----------------------------------RTPGHVDFSYEVSRSLAACQGPLLIV 120
                                              TPGHVDFSYEVSRSL+ACQG LL+V
Sbjct: 109 ERERGITVKAQTATMFYENKVEDQEASGYLLNLIDTPGHVDFSYEVSRSLSACQGALLVV 168

Query: 121 DAAQ-------------------------------------------------------- 180
           DAAQ                                                        
Sbjct: 169 DAAQGVQAQTVANFYLAFEANLTIVPVINKIDQPTADPERVKAQLKSMFDLDTEDVLLVS 228

Query: 181 ---GQGLEHVLPAIIEHIPPPPGKSSLPLRMLLLDLYYDEYKGVICHVAVVGGVLRKGDK 240
              G GLEHVLPA+IE IPPPPG S  PLRMLL D +++EYKGVIC+V+VV G+L KGDK
Sbjct: 229 AKTGLGLEHVLPAVIERIPPPPGISESPLRMLLFDSFFNEYKGVICYVSVVDGMLSKGDK 288

Query: 241 ISSVGTGHAFEVLDVGIMHPELTSTGILLTSQVGYVVSGMRSTKEARIGDTLHQSKSVVE 272
           +S   +G ++EVLDVGIMHPELTSTG+LLT QVGY+V+GMR+TKEARIGDT++++K+ VE
Sbjct: 289 VSFAASGQSYEVLDVGIMHPELTSTGMLLTGQVGYIVTGMRTTKEARIGDTIYRTKTTVE 348

HSP 2 Score: 63.5 bits (153), Expect = 5.2e-09
Identity = 30/34 (88.24%), Postives = 32/34 (94.12%), Query Frame = 0


Query: 293 ISATRKNVLAKCYGGDVTRKRKLLEKQKEGKKCM 327
           ISA RKNVLAKCYGGD+TRK+KLLEKQKEGKK M
Sbjct: 610 ISAMRKNVLAKCYGGDITRKKKLLEKQKEGKKRM 643

[Close]

BLAST of Cp4.1LG13g08620 vs. NCBI nr
Match: XP_038890032.1 (translation factor GUF1 homolog, mitochondrial isoform X4 [Benincasa hispida])

HSP 1 Score: 433 bits (1114), Expect = 5.01e-146
Identity = 240/368 (65.22%), Postives = 247/368 (67.12%), Query Frame = 0


Query: 1   QNVEEVGIDLTQFPPDRIRNFSIIAHVDHGKYTLADRLLELPGTIKRGHGRPLYLDKLQ- 60
           QNV+EVGIDLTQ+PP+RIRNFSIIAHVDHGK TLADRLLEL GTIKRGHG+P YLDKLQ 
Sbjct: 51  QNVKEVGIDLTQYPPERIRNFSIIAHVDHGKSTLADRLLELTGTIKRGHGQPQYLDKLQV 110

Query: 61  -------------------------------------RTPGHVDFSYEVSRSLAACQGPL 120
                                                 TPGHVDFSYEVSRSLAACQG L
Sbjct: 111 ERERGITVKAQTATMFHKHNLVGNNTSEQPPFLINLIDTPGHVDFSYEVSRSLAACQGAL 170

Query: 121 LIVDAAQG---------------------------------------------------- 180
           L+VDAAQG                                                    
Sbjct: 171 LVVDAAQGVQAQTVANFYLAFESNLAIIPVINKIDQPTADPDRVKAQLKSMFDLEPTHAL 230

Query: 181 -------QGLEHVLPAIIEHIPPPPGKSSLPLRMLLLDLYYDEYKGVICHVAVVGGVLRK 240
                  QGLEHVLPAIIE IPPPPGKSS PLRMLLLD YYDEYKGVICHVAVV GVL K
Sbjct: 231 LTSAKTGQGLEHVLPAIIERIPPPPGKSSSPLRMLLLDSYYDEYKGVICHVAVVDGVLHK 290

Query: 241 GDKISSVGTGHAFEVLDVGIMHPELTSTGILLTSQVGYVVSGMRSTKEARIGDTLHQSKS 271
           GDKISS  TG A+EVLDVGIMHPELTSTGILLT QVGYVVSGMRSTKEARIGDTLHQSK+
Sbjct: 291 GDKISSAATGQAYEVLDVGIMHPELTSTGILLTGQVGYVVSGMRSTKEARIGDTLHQSKT 350

[Close]

BLAST of Cp4.1LG13g08620 vs. NCBI nr
Match: XP_038890029.1 (translation factor GUF1 homolog, mitochondrial isoform X2 [Benincasa hispida])

HSP 1 Score: 433 bits (1114), Expect = 1.18e-145
Identity = 240/368 (65.22%), Postives = 247/368 (67.12%), Query Frame = 0


Query: 1   QNVEEVGIDLTQFPPDRIRNFSIIAHVDHGKYTLADRLLELPGTIKRGHGRPLYLDKLQ- 60
           QNV+EVGIDLTQ+PP+RIRNFSIIAHVDHGK TLADRLLEL GTIKRGHG+P YLDKLQ 
Sbjct: 51  QNVKEVGIDLTQYPPERIRNFSIIAHVDHGKSTLADRLLELTGTIKRGHGQPQYLDKLQV 110

Query: 61  -------------------------------------RTPGHVDFSYEVSRSLAACQGPL 120
                                                 TPGHVDFSYEVSRSLAACQG L
Sbjct: 111 ERERGITVKAQTATMFHKHNLVGNNTSEQPPFLINLIDTPGHVDFSYEVSRSLAACQGAL 170

Query: 121 LIVDAAQG---------------------------------------------------- 180
           L+VDAAQG                                                    
Sbjct: 171 LVVDAAQGVQAQTVANFYLAFESNLAIIPVINKIDQPTADPDRVKAQLKSMFDLEPTHAL 230

Query: 181 -------QGLEHVLPAIIEHIPPPPGKSSLPLRMLLLDLYYDEYKGVICHVAVVGGVLRK 240
                  QGLEHVLPAIIE IPPPPGKSS PLRMLLLD YYDEYKGVICHVAVV GVL K
Sbjct: 231 LTSAKTGQGLEHVLPAIIERIPPPPGKSSSPLRMLLLDSYYDEYKGVICHVAVVDGVLHK 290

Query: 241 GDKISSVGTGHAFEVLDVGIMHPELTSTGILLTSQVGYVVSGMRSTKEARIGDTLHQSKS 271
           GDKISS  TG A+EVLDVGIMHPELTSTGILLT QVGYVVSGMRSTKEARIGDTLHQSK+
Sbjct: 291 GDKISSAATGQAYEVLDVGIMHPELTSTGILLTGQVGYVVSGMRSTKEARIGDTLHQSKT 350

[Close]

BLAST of Cp4.1LG13g08620 vs. NCBI nr
Match: XP_038890027.1 (translation factor GUF1 homolog, mitochondrial isoform X1 [Benincasa hispida] >XP_038890028.1 translation factor GUF1 homolog, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 433 bits (1114), Expect = 1.84e-144
Identity = 240/368 (65.22%), Postives = 247/368 (67.12%), Query Frame = 0


Query: 1   QNVEEVGIDLTQFPPDRIRNFSIIAHVDHGKYTLADRLLELPGTIKRGHGRPLYLDKLQ- 60
           QNV+EVGIDLTQ+PP+RIRNFSIIAHVDHGK TLADRLLEL GTIKRGHG+P YLDKLQ 
Sbjct: 51  QNVKEVGIDLTQYPPERIRNFSIIAHVDHGKSTLADRLLELTGTIKRGHGQPQYLDKLQV 110

Query: 61  -------------------------------------RTPGHVDFSYEVSRSLAACQGPL 120
                                                 TPGHVDFSYEVSRSLAACQG L
Sbjct: 111 ERERGITVKAQTATMFHKHNLVGNNTSEQPPFLINLIDTPGHVDFSYEVSRSLAACQGAL 170

Query: 121 LIVDAAQG---------------------------------------------------- 180
           L+VDAAQG                                                    
Sbjct: 171 LVVDAAQGVQAQTVANFYLAFESNLAIIPVINKIDQPTADPDRVKAQLKSMFDLEPTHAL 230

Query: 181 -------QGLEHVLPAIIEHIPPPPGKSSLPLRMLLLDLYYDEYKGVICHVAVVGGVLRK 240
                  QGLEHVLPAIIE IPPPPGKSS PLRMLLLD YYDEYKGVICHVAVV GVL K
Sbjct: 231 LTSAKTGQGLEHVLPAIIERIPPPPGKSSSPLRMLLLDSYYDEYKGVICHVAVVDGVLHK 290

Query: 241 GDKISSVGTGHAFEVLDVGIMHPELTSTGILLTSQVGYVVSGMRSTKEARIGDTLHQSKS 271
           GDKISS  TG A+EVLDVGIMHPELTSTGILLT QVGYVVSGMRSTKEARIGDTLHQSK+
Sbjct: 291 GDKISSAATGQAYEVLDVGIMHPELTSTGILLTGQVGYVVSGMRSTKEARIGDTLHQSKT 350

[Close]

BLAST of Cp4.1LG13g08620 vs. NCBI nr
Match: XP_011655990.1 (translation factor GUF1 homolog, mitochondrial isoform X1 [Cucumis sativus] >KAE8648915.1 hypothetical protein Csa_008764 [Cucumis sativus])

HSP 1 Score: 427 bits (1097), Expect = 6.71e-142
Identity = 236/368 (64.13%), Postives = 246/368 (66.85%), Query Frame = 0


Query: 1   QNVEEVGIDLTQFPPDRIRNFSIIAHVDHGKYTLADRLLELPGTIKRGHGRPLYLDKLQ- 60
           QN++E GIDLTQ+PP+RIRNFSIIAHVDHGK TLADRLLEL GTIKRGHG+P YLDKLQ 
Sbjct: 51  QNLKEAGIDLTQYPPERIRNFSIIAHVDHGKSTLADRLLELTGTIKRGHGQPQYLDKLQV 110

Query: 61  -------------------------------------RTPGHVDFSYEVSRSLAACQGPL 120
                                                 TPGHVDFSYEVSRSLAACQG L
Sbjct: 111 ERERGITVKAQTATMFHKCNLVGDNTSEQPPFLINLIDTPGHVDFSYEVSRSLAACQGAL 170

Query: 121 LIVDAAQG---------------------------------------------------- 180
           L+VDAAQG                                                    
Sbjct: 171 LVVDAAQGVQAQTVANFYLAFESNLTVIPVINKIDQPTADPDRVKAQLKSMFDLEPTHAL 230

Query: 181 -------QGLEHVLPAIIEHIPPPPGKSSLPLRMLLLDLYYDEYKGVICHVAVVGGVLRK 240
                  QGLE VLPAIIE IPPPPGKS+ PLRMLLLD YYDEYKGVICHVAVV GVLRK
Sbjct: 231 LTSAKTGQGLEQVLPAIIERIPPPPGKSTSPLRMLLLDSYYDEYKGVICHVAVVDGVLRK 290

Query: 241 GDKISSVGTGHAFEVLDVGIMHPELTSTGILLTSQVGYVVSGMRSTKEARIGDTLHQSKS 271
           GDKISS  TG A+EVLDVGIMHPELTSTGILLT QVGYVVSGMRSTKEARIGDTLHQSK+
Sbjct: 291 GDKISSAATGQAYEVLDVGIMHPELTSTGILLTGQVGYVVSGMRSTKEARIGDTLHQSKT 350

[Close]

BLAST of Cp4.1LG13g08620 vs. NCBI nr
Match: XP_022991182.1 (translation factor GUF1 homolog, mitochondrial isoform X1 [Cucurbita maxima] >XP_022991183.1 translation factor GUF1 homolog, mitochondrial isoform X1 [Cucurbita maxima])

HSP 1 Score: 426 bits (1096), Expect = 9.50e-142
Identity = 237/368 (64.40%), Postives = 245/368 (66.58%), Query Frame = 0


Query: 1   QNVEEVGIDLTQFPPDRIRNFSIIAHVDHGKYTLADRLLELPGTIKRGHGRPLYLDKLQ- 60
           Q  +EV IDL+Q+PP+RIRNFSIIAHVDHGK TLADRLLEL GTIKRGHG+P YLDKLQ 
Sbjct: 51  QKSKEVDIDLSQYPPERIRNFSIIAHVDHGKSTLADRLLELTGTIKRGHGQPQYLDKLQV 110

Query: 61  -------------------------------------RTPGHVDFSYEVSRSLAACQGPL 120
                                                 TPGHVDFSYEVSRSLAACQG L
Sbjct: 111 ERERGITVKAQTATMFHKYNPVGDNASEQPPFLINLIDTPGHVDFSYEVSRSLAACQGAL 170

Query: 121 LIVDAAQG---------------------------------------------------- 180
           L+VDAAQG                                                    
Sbjct: 171 LVVDAAQGVQAQTVANFYLAFESNLTIIPVINKIDQPTADPDRVKAQLKSMFDLDSAHAV 230

Query: 181 -------QGLEHVLPAIIEHIPPPPGKSSLPLRMLLLDLYYDEYKGVICHVAVVGGVLRK 240
                  QGLEHVLPAIIE IPPPPGKSS PLRMLLLD YYDEYKGVICHVAVV GVLRK
Sbjct: 231 LTSAKTGQGLEHVLPAIIERIPPPPGKSSSPLRMLLLDSYYDEYKGVICHVAVVDGVLRK 290

Query: 241 GDKISSVGTGHAFEVLDVGIMHPELTSTGILLTSQVGYVVSGMRSTKEARIGDTLHQSKS 271
           GDKISS  TG A+EVLDVGIMHPELTSTGILLT QVGYVVSGMRSTKEARIGDTLHQSK+
Sbjct: 291 GDKISSAATGQAYEVLDVGIMHPELTSTGILLTGQVGYVVSGMRSTKEARIGDTLHQSKT 350

[Close]

BLAST of Cp4.1LG13g08620 vs. ExPASy TrEMBL
Match: A0A6J1JL24 (Translation factor GUF1 homolog, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111487856 PE=3 SV=1)

HSP 1 Score: 426 bits (1096), Expect = 4.60e-142
Identity = 237/368 (64.40%), Postives = 245/368 (66.58%), Query Frame = 0


Query: 1   QNVEEVGIDLTQFPPDRIRNFSIIAHVDHGKYTLADRLLELPGTIKRGHGRPLYLDKLQ- 60
           Q  +EV IDL+Q+PP+RIRNFSIIAHVDHGK TLADRLLEL GTIKRGHG+P YLDKLQ 
Sbjct: 51  QKSKEVDIDLSQYPPERIRNFSIIAHVDHGKSTLADRLLELTGTIKRGHGQPQYLDKLQV 110

Query: 61  -------------------------------------RTPGHVDFSYEVSRSLAACQGPL 120
                                                 TPGHVDFSYEVSRSLAACQG L
Sbjct: 111 ERERGITVKAQTATMFHKYNPVGDNASEQPPFLINLIDTPGHVDFSYEVSRSLAACQGAL 170

Query: 121 LIVDAAQG---------------------------------------------------- 180
           L+VDAAQG                                                    
Sbjct: 171 LVVDAAQGVQAQTVANFYLAFESNLTIIPVINKIDQPTADPDRVKAQLKSMFDLDSAHAV 230

Query: 181 -------QGLEHVLPAIIEHIPPPPGKSSLPLRMLLLDLYYDEYKGVICHVAVVGGVLRK 240
                  QGLEHVLPAIIE IPPPPGKSS PLRMLLLD YYDEYKGVICHVAVV GVLRK
Sbjct: 231 LTSAKTGQGLEHVLPAIIERIPPPPGKSSSPLRMLLLDSYYDEYKGVICHVAVVDGVLRK 290

Query: 241 GDKISSVGTGHAFEVLDVGIMHPELTSTGILLTSQVGYVVSGMRSTKEARIGDTLHQSKS 271
           GDKISS  TG A+EVLDVGIMHPELTSTGILLT QVGYVVSGMRSTKEARIGDTLHQSK+
Sbjct: 291 GDKISSAATGQAYEVLDVGIMHPELTSTGILLTGQVGYVVSGMRSTKEARIGDTLHQSKT 350

[Close]

BLAST of Cp4.1LG13g08620 vs. ExPASy TrEMBL
Match: A0A1S3C9C6 (Translation factor GUF1 homolog, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103498472 PE=3 SV=1)

HSP 1 Score: 425 bits (1093), Expect = 1.30e-141
Identity = 235/368 (63.86%), Postives = 245/368 (66.58%), Query Frame = 0


Query: 1   QNVEEVGIDLTQFPPDRIRNFSIIAHVDHGKYTLADRLLELPGTIKRGHGRPLYLDKLQ- 60
           QN++E GIDLTQ+PP+RIRNFSIIAHVDHGK TLADRLLEL GTIKRGHG+P YLDKLQ 
Sbjct: 51  QNLKEAGIDLTQYPPERIRNFSIIAHVDHGKSTLADRLLELTGTIKRGHGQPQYLDKLQV 110

Query: 61  -------------------------------------RTPGHVDFSYEVSRSLAACQGPL 120
                                                 TPGHVDFSYEVSRSLAACQG L
Sbjct: 111 ERERGITVKAQTATMFHKCNLVGDNTSEQPPFLINLIDTPGHVDFSYEVSRSLAACQGAL 170

Query: 121 LIVDAAQG---------------------------------------------------- 180
           L+VDAAQG                                                    
Sbjct: 171 LVVDAAQGVQAQTVANFYLAFESNLTIIPVINKIDQPTADPDRVKAQLKSMFDLEPTHAL 230

Query: 181 -------QGLEHVLPAIIEHIPPPPGKSSLPLRMLLLDLYYDEYKGVICHVAVVGGVLRK 240
                  QGLE VLPAIIE IPPPPGKS+ PLRMLLLD YYDEYKGVICHVAVV GVLRK
Sbjct: 231 LTSAKTGQGLEQVLPAIIERIPPPPGKSTSPLRMLLLDSYYDEYKGVICHVAVVDGVLRK 290

Query: 241 GDKISSVGTGHAFEVLDVGIMHPELTSTGILLTSQVGYVVSGMRSTKEARIGDTLHQSKS 271
           GDKISS  TG A+EVLDVGIMHPELTSTGILLT QVGYVVSGMRSTKEARIGDTLHQSK+
Sbjct: 291 GDKISSAATGQAYEVLDVGIMHPELTSTGILLTGQVGYVVSGMRSTKEARIGDTLHQSKT 350

[Close]

BLAST of Cp4.1LG13g08620 vs. ExPASy TrEMBL
Match: A0A6J1HK65 (Translation factor GUF1 homolog, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111464855 PE=3 SV=1)

HSP 1 Score: 425 bits (1093), Expect = 1.30e-141
Identity = 236/368 (64.13%), Postives = 245/368 (66.58%), Query Frame = 0


Query: 1   QNVEEVGIDLTQFPPDRIRNFSIIAHVDHGKYTLADRLLELPGTIKRGHGRPLYLDKLQ- 60
           Q  +EV IDL+Q+PP+RIRNFSIIAHVDHGK TLADRLLEL GTIKRGHG+P YLDKLQ 
Sbjct: 51  QKSKEVDIDLSQYPPERIRNFSIIAHVDHGKSTLADRLLELTGTIKRGHGQPQYLDKLQV 110

Query: 61  -------------------------------------RTPGHVDFSYEVSRSLAACQGPL 120
                                                 TPGHVDFSYEVSRSLAACQG L
Sbjct: 111 ERERGITVKAQTATMFHKYNPVGDNASEQPPFLINLIDTPGHVDFSYEVSRSLAACQGAL 170

Query: 121 LIVDAAQG---------------------------------------------------- 180
           L+VDAAQG                                                    
Sbjct: 171 LVVDAAQGVQAQTVANFYLAFESNLTIIPVINKIDQPTADPDRVKAQLKSMFDLDSAHAL 230

Query: 181 -------QGLEHVLPAIIEHIPPPPGKSSLPLRMLLLDLYYDEYKGVICHVAVVGGVLRK 240
                  QGLEHVLPAIIE IPPPPGKSS PLRMLLLD YYDEYKGVICHVAVV GVLRK
Sbjct: 231 LTSAKTGQGLEHVLPAIIERIPPPPGKSSSPLRMLLLDSYYDEYKGVICHVAVVDGVLRK 290

Query: 241 GDKISSVGTGHAFEVLDVGIMHPELTSTGILLTSQVGYVVSGMRSTKEARIGDTLHQSKS 271
           GDKISS  TG A+EVLDVGIMHPELTSTGILLT QVGYVVSGMRSTKEARIGDTLHQSK+
Sbjct: 291 GDKISSAATGQAYEVLDVGIMHPELTSTGILLTGQVGYVVSGMRSTKEARIGDTLHQSKT 350

[Close]

BLAST of Cp4.1LG13g08620 vs. ExPASy TrEMBL
Match: A0A5D3CYM9 (Translation factor GUF1 homolog, mitochondrial OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold892G00460 PE=3 SV=1)

HSP 1 Score: 424 bits (1089), Expect = 3.88e-141
Identity = 234/368 (63.59%), Postives = 245/368 (66.58%), Query Frame = 0


Query: 1   QNVEEVGIDLTQFPPDRIRNFSIIAHVDHGKYTLADRLLELPGTIKRGHGRPLYLDKLQ- 60
           QN++E GIDLT++PP+RIRNFSIIAHVDHGK TLADRLLEL GTIKRGHG+P YLDKLQ 
Sbjct: 41  QNLKEAGIDLTKYPPERIRNFSIIAHVDHGKSTLADRLLELTGTIKRGHGQPQYLDKLQV 100

Query: 61  -------------------------------------RTPGHVDFSYEVSRSLAACQGPL 120
                                                 TPGHVDFSYEVSRSLAACQG L
Sbjct: 101 ERERGITVKAQTATMFHKCNLVGDNTSEQPPFLINLIDTPGHVDFSYEVSRSLAACQGAL 160

Query: 121 LIVDAAQG---------------------------------------------------- 180
           L+VDAAQG                                                    
Sbjct: 161 LVVDAAQGVQAQTVANFYLAFESNLTIIPVINKIDQPTADPDRVKAQLKSMFDLEPTHAL 220

Query: 181 -------QGLEHVLPAIIEHIPPPPGKSSLPLRMLLLDLYYDEYKGVICHVAVVGGVLRK 240
                  QGLE VLPAIIE IPPPPGKS+ PLRMLLLD YYDEYKGVICHVAVV GVLRK
Sbjct: 221 LTSAKTGQGLEQVLPAIIERIPPPPGKSTSPLRMLLLDSYYDEYKGVICHVAVVDGVLRK 280

Query: 241 GDKISSVGTGHAFEVLDVGIMHPELTSTGILLTSQVGYVVSGMRSTKEARIGDTLHQSKS 271
           GDKISS  TG A+EVLDVGIMHPELTSTGILLT QVGYVVSGMRSTKEARIGDTLHQSK+
Sbjct: 281 GDKISSAATGQAYEVLDVGIMHPELTSTGILLTGQVGYVVSGMRSTKEARIGDTLHQSKT 340

[Close]

BLAST of Cp4.1LG13g08620 vs. ExPASy TrEMBL
Match: A0A0A0KVI1 (Translation factor GUF1 homolog, mitochondrial OS=Cucumis sativus OX=3659 GN=Csa_5G635420 PE=3 SV=1)

HSP 1 Score: 427 bits (1097), Expect = 5.35e-141
Identity = 236/368 (64.13%), Postives = 246/368 (66.85%), Query Frame = 0


Query: 1   QNVEEVGIDLTQFPPDRIRNFSIIAHVDHGKYTLADRLLELPGTIKRGHGRPLYLDKLQ- 60
           QN++E GIDLTQ+PP+RIRNFSIIAHVDHGK TLADRLLEL GTIKRGHG+P YLDKLQ 
Sbjct: 149 QNLKEAGIDLTQYPPERIRNFSIIAHVDHGKSTLADRLLELTGTIKRGHGQPQYLDKLQV 208

Query: 61  -------------------------------------RTPGHVDFSYEVSRSLAACQGPL 120
                                                 TPGHVDFSYEVSRSLAACQG L
Sbjct: 209 ERERGITVKAQTATMFHKCNLVGDNTSEQPPFLINLIDTPGHVDFSYEVSRSLAACQGAL 268

Query: 121 LIVDAAQG---------------------------------------------------- 180
           L+VDAAQG                                                    
Sbjct: 269 LVVDAAQGVQAQTVANFYLAFESNLTVIPVINKIDQPTADPDRVKAQLKSMFDLEPTHAL 328

Query: 181 -------QGLEHVLPAIIEHIPPPPGKSSLPLRMLLLDLYYDEYKGVICHVAVVGGVLRK 240
                  QGLE VLPAIIE IPPPPGKS+ PLRMLLLD YYDEYKGVICHVAVV GVLRK
Sbjct: 329 LTSAKTGQGLEQVLPAIIERIPPPPGKSTSPLRMLLLDSYYDEYKGVICHVAVVDGVLRK 388

Query: 241 GDKISSVGTGHAFEVLDVGIMHPELTSTGILLTSQVGYVVSGMRSTKEARIGDTLHQSKS 271
           GDKISS  TG A+EVLDVGIMHPELTSTGILLT QVGYVVSGMRSTKEARIGDTLHQSK+
Sbjct: 389 GDKISSAATGQAYEVLDVGIMHPELTSTGILLTGQVGYVVSGMRSTKEARIGDTLHQSKT 448

[Close]

BLAST of Cp4.1LG13g08620 vs. TAIR 10
Match: AT5G39900.1 (Small GTP-binding protein )

HSP 1 Score: 370.9 bits (951), Expect = 1.1e-102
Identity = 198/365 (54.25%), Postives = 235/365 (64.38%), Query Frame = 0


Query: 1   QNVEEVGIDLTQFPPDRIRNFSIIAHVDHGKYTLADRLLELPGTIKRGHGRPLYLDKLQ- 60
           Q+ +E  IDLT+FP ++IRNFSIIAH+DHGK TLADRL+EL GTIK+GHG+P YLDKLQ 
Sbjct: 49  QSSKEPTIDLTKFPSEKIRNFSIIAHIDHGKSTLADRLMELTGTIKKGHGQPQYLDKLQV 108

Query: 61  ----------------------------------RTPGHVDFSYEVSRSLAACQGPLLIV 120
                                              TPGHVDFSYEVSRSL+ACQG LL+V
Sbjct: 109 ERERGITVKAQTATMFYENKVEDQEASGYLLNLIDTPGHVDFSYEVSRSLSACQGALLVV 168

Query: 121 DAAQ-------------------------------------------------------- 180
           DAAQ                                                        
Sbjct: 169 DAAQGVQAQTVANFYLAFEANLTIVPVINKIDQPTADPERVKAQLKSMFDLDTEDVLLVS 228

Query: 181 ---GQGLEHVLPAIIEHIPPPPGKSSLPLRMLLLDLYYDEYKGVICHVAVVGGVLRKGDK 240
              G GLEHVLPA+IE IPPPPG S  PLRMLL D +++EYKGVIC+V+VV G+L KGDK
Sbjct: 229 AKTGLGLEHVLPAVIERIPPPPGISESPLRMLLFDSFFNEYKGVICYVSVVDGMLSKGDK 288

Query: 241 ISSVGTGHAFEVLDVGIMHPELTSTGILLTSQVGYVVSGMRSTKEARIGDTLHQSKSVVE 272
           +S   +G ++EVLDVGIMHPELTSTG+LLT QVGY+V+GMR+TKEARIGDT++++K+ VE
Sbjct: 289 VSFAASGQSYEVLDVGIMHPELTSTGMLLTGQVGYIVTGMRTTKEARIGDTIYRTKTTVE 348

HSP 2 Score: 63.5 bits (153), Expect = 3.7e-10
Identity = 30/34 (88.24%), Postives = 32/34 (94.12%), Query Frame = 0


Query: 293 ISATRKNVLAKCYGGDVTRKRKLLEKQKEGKKCM 327
           ISA RKNVLAKCYGGD+TRK+KLLEKQKEGKK M
Sbjct: 610 ISAMRKNVLAKCYGGDITRKKKLLEKQKEGKKRM 643

[Close]

BLAST of Cp4.1LG13g08620 vs. TAIR 10
Match: AT5G08650.1 (Small GTP-binding protein )

HSP 1 Score: 199.1 bits (505), Expect = 5.6e-51
Identity = 121/352 (34.38%), Postives = 168/352 (47.73%), Query Frame = 0


Query: 10  LTQFPPDRIRNFSIIAHVDHGKYTLADRLLELPGTIKRGHGRPLYLD------------K 69
           L + P   IRNFSIIAH+DHGK TLAD+LL++ GT++    +  +LD            K
Sbjct: 78  LLKVPISNIRNFSIIAHIDHGKSTLADKLLQVTGTVQNRDMKEQFLDNMDLERERGITIK 137

Query: 70  LQR------------------TPGHVDFSYEVSRSLAACQGPLLIVDAAQGQ-------- 129
           LQ                   TPGHVDFSYEVSRSLAAC+G LL+VDA+QG         
Sbjct: 138 LQAARMRYVYEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQGVEAQTLANV 197

Query: 130 ---------------------------------------------------GLEHVLPAI 189
                                                              G+  +L AI
Sbjct: 198 YLALENNLEIIPVLNKIDLPGAEPEKVLREIEEVIGLDCSKAIFCSAKEGIGITEILDAI 257

Query: 190 IEHIPPPPGKSSLPLRMLLLDLYYDEYKGVICHVAVVGGVLRKGDKISSVGTGHAFEVLD 249
           ++ IP P   +  PLR L+ D YYD Y+GVI +  V+ G ++KGD+I  + +G  +   +
Sbjct: 258 VQRIPAPLDTAGKPLRALIFDSYYDPYRGVIVYFRVIDGKVKKGDRIFFMASGKDYFADE 317

Query: 250 VGIMHPELTSTGILLTSQVGYVVSGMRSTKEARIGDTL-HQSKSVVEPLPLTFVLLVGFK 272
           VG++ P       L   +VGY+ + +RS  +AR+GDT+ H S+     LP       G++
Sbjct: 318 VGVLSPNQIQVDELYAGEVGYIAASVRSVADARVGDTITHYSRKAESSLP-------GYE 377

HSP 2 Score: 53.5 bits (127), Expect = 3.8e-07
Identity = 26/48 (54.17%), Postives = 36/48 (75.00%), Query Frame = 0


Query: 293 ISATRKNVLAKCYGGDVTRKRKLLEKQKEGKKCMNVLDLLIFLRKQFM 341
           +SA RK+VLAKCYGGD++RK+KLL+KQ  GKK M  +  +   ++ FM
Sbjct: 624 LSAIRKDVLAKCYGGDISRKKKLLKKQAAGKKRMKAIGRVDVPQEAFM 671

[Close]

BLAST of Cp4.1LG13g08620 vs. TAIR 10
Match: AT2G31060.2 (elongation factor family protein )

HSP 1 Score: 64.3 bits (155), Expect = 2.2e-10
Identity = 39/108 (36.11%), Postives = 51/108 (47.22%), Query Frame = 0


Query: 7   GIDLTQFPPDRIRNFSIIAHVDHGKYTLADRLLELPGTIKRGHGRPLYLDKLQR------ 66
           G   +   P+R+RN ++IAHVDHGK TL DRLL   G     H R +    L+R      
Sbjct: 48  GAPNSSLDPNRLRNVAVIAHVDHGKTTLMDRLLRQCGA-DIPHERAMDSINLERERGITI 107

Query: 67  ------------------TPGHVDFSYEVSRSLAACQGPLLIVDAAQG 91
                             TPGH DF  EV R +   +G +L+VDA +G
Sbjct: 108 SSKVTSIFWKDNELNMVDTPGHADFGGEVERVVGMVEGAILVVDAGEG 154

[Close]

BLAST of Cp4.1LG13g08620 vs. TAIR 10
Match: AT2G31060.3 (elongation factor family protein )

HSP 1 Score: 62.8 bits (151), Expect = 6.3e-10
Identity = 39/112 (34.82%), Postives = 51/112 (45.54%), Query Frame = 0


Query: 7   GIDLTQFPPDRIRNFSIIAHVDHGKYTLADRLLELPGTIKRGHGRPLYLDKLQR------ 66
           G   +   P+R+RN ++IAHVDHGK TL DRLL   G     H R +    L+R      
Sbjct: 48  GAPNSSLDPNRLRNVAVIAHVDHGKTTLMDRLLRQCGA-DIPHERAMDSINLERERGITI 107

Query: 67  ----------------------TPGHVDFSYEVSRSLAACQGPLLIVDAAQG 91
                                 TPGH DF  EV R +   +G +L+VDA +G
Sbjct: 108 SSKVSPFVTSIFWKDNELNMVDTPGHADFGGEVERVVGMVEGAILVVDAGEG 158

[Close]

BLAST of Cp4.1LG13g08620 vs. TAIR 10
Match: AT3G22980.1 (Ribosomal protein S5/Elongation factor G/III/V family protein )

HSP 1 Score: 54.3 bits (129), Expect = 2.2e-07
Identity = 37/103 (35.92%), Postives = 49/103 (47.57%), Query Frame = 0


Query: 17  RIRNFSIIAHVDHGKYTLADRLLELPG-----TIKRGHGRPL-YLDKLQR---------- 76
           ++RN  I+AHVDHGK TLAD L+   G         G  R + YLD+ QR          
Sbjct: 8   KVRNICILAHVDHGKTTLADHLIASSGGGVLHPRLAGKLRFMDYLDEEQRRAITMKSSSI 67

Query: 77  -------------TPGHVDFSYEVSRSLAACQGPLLIVDAAQG 91
                        +PGH+DF  EVS +     G L++VDA +G
Sbjct: 68  SLKYKDYSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110

The following BLAST results are available for this feature:

BLAST of Cp4.1LG13g08620 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B9RUN8	1.9e-107	59.07	Translation factor GUF1 homolog, mitochondrial OS=Ricinus communis OX=3988 GN=RC...	[more]
B8B2R1	6.0e-106	57.10	Translation factor GUF1 homolog, mitochondrial OS=Oryza sativa subsp. indica OX=...	[more]
Q5VQ69	1.3e-105	57.10	Translation factor GUF1 homolog, mitochondrial OS=Oryza sativa subsp. japonica O...	[more]
C5Z3W1	1.7e-105	57.34	Translation factor GUF1 homolog, mitochondrial OS=Sorghum bicolor OX=4558 GN=Sb1...	[more]
Q9FLE4	1.5e-101	54.25	Translation factor GUF1 homolog, mitochondrial OS=Arabidopsis thaliana OX=3702 G...	[more]

BLAST of Cp4.1LG13g08620 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038890032.1	5.01e-146	65.22	translation factor GUF1 homolog, mitochondrial isoform X4 [Benincasa hispida]	[more]
XP_038890029.1	1.18e-145	65.22	translation factor GUF1 homolog, mitochondrial isoform X2 [Benincasa hispida]	[more]
XP_038890027.1	1.84e-144	65.22	translation factor GUF1 homolog, mitochondrial isoform X1 [Benincasa hispida] >X...	[more]
XP_011655990.1	6.71e-142	64.13	translation factor GUF1 homolog, mitochondrial isoform X1 [Cucumis sativus] >KAE...	[more]
XP_022991182.1	9.50e-142	64.40	translation factor GUF1 homolog, mitochondrial isoform X1 [Cucurbita maxima] >XP...	[more]

BLAST of Cp4.1LG13g08620 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JL24	4.60e-142	64.40	Translation factor GUF1 homolog, mitochondrial OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A1S3C9C6	1.30e-141	63.86	Translation factor GUF1 homolog, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A6J1HK65	1.30e-141	64.13	Translation factor GUF1 homolog, mitochondrial OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A5D3CYM9	3.88e-141	63.59	Translation factor GUF1 homolog, mitochondrial OS=Cucumis melo var. makuwa OX=11...	[more]
A0A0A0KVI1	5.35e-141	64.13	Translation factor GUF1 homolog, mitochondrial OS=Cucumis sativus OX=3659 GN=Csa...	[more]

BLAST of Cp4.1LG13g08620 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G39900.1	1.1e-102	54.25	Small GTP-binding protein	[more]
AT5G08650.1	5.6e-51	34.38	Small GTP-binding protein	[more]
AT2G31060.2	2.2e-10	36.11	elongation factor family protein	[more]
AT2G31060.3	6.3e-10	34.82	elongation factor family protein	[more]
AT3G22980.1	2.2e-07	35.92	Ribosomal protein S5/Elongation factor G/III/V family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.70.870	Elongation Factor G (Translational Gtpase), domain 3	coord: 216..275 e-value: 5.0E-14 score: 53.9
None	No IPR available	GENE3D	2.40.30.10	Translation factors	coord: 111..208 e-value: 7.6E-30 score: 104.8
None	No IPR available	PANTHER	PTHR43512:SF4	TRANSLATION FACTOR GUF1, MITOCHONDRIAL	coord: 89..271
None	No IPR available	PANTHER	PTHR43512:SF4	TRANSLATION FACTOR GUF1, MITOCHONDRIAL	coord: 292..328
None	No IPR available	PANTHER	PTHR43512:SF4	TRANSLATION FACTOR GUF1, MITOCHONDRIAL	coord: 61..90 coord: 7..59
IPR000795	Translational (tr)-type GTP-binding domain	PFAM	PF00009	GTP_EFTU	coord: 61..96 e-value: 3.9E-7 score: 29.8 coord: 17..49 e-value: 1.5E-7 score: 31.2
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 59..106 e-value: 5.9E-7 score: 30.8
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 9..58 e-value: 1.0E-8 score: 37.0
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 14..91
IPR013842	GTP-binding protein LepA, C-terminal	PFAM	PF06421	LepA_C	coord: 292..333 e-value: 1.8E-14 score: 53.8
IPR006297	Elongation factor 4	PANTHER	PTHR43512	TRANSLATION FACTOR GUF1-RELATED	coord: 61..90 coord: 292..328
IPR006297	Elongation factor 4	PANTHER	PTHR43512	TRANSLATION FACTOR GUF1-RELATED	coord: 7..59
IPR006297	Elongation factor 4	PANTHER	PTHR43512	TRANSLATION FACTOR GUF1-RELATED	coord: 89..271
IPR009000	Translation protein, beta-barrel domain superfamily	SUPERFAMILY	50447	Translation proteins	coord: 90..205

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG13g08620.1	Cp4.1LG13g08620.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0003924	GTPase activity
molecular_function	GO:0005525	GTP binding
molecular_function	GO:0043022	ribosome binding