Cp4.1LG13g06780 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG13g06780
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionadenylate isopentenyltransferase-like
LocationCp4.1LG13: 4430977 .. 4431984 (+)
RNA-Seq ExpressionCp4.1LG13g06780
SyntenyCp4.1LG13g06780
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACACTTTCCCTCTCCTTCTCCCATTTTAATGCCCCTTTTTCCTGTTCTTCCCGCCGCCCCCGATCCCCACTTGTCCTCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCCCCTCCCCCTCCCATAATCTCATCCTCCTCCTCGGCGCCACCGGCTCCGGCAAGTCCTCCCTCTCCATTGACCTCGCCTCCCGCTTCCCCTCCGAGATTATTAATTCTGACAAAATGCAACTCTACACAGGCCTCGACATCACCACTAACAAGCTCCCCCTCTCCGACCGCCGCGCCGTCCCCCACCATCTTCTCGGCGACTTCGATCCTCTCGCCGGCGAAGTCTCCGCATCAGCATTCCGCTCCCACGCCGCGGCCGTTATCTCCGGCGTAGTCTCCCGAGGGAACCTTCCTCTCGTCGTCGGCGGTTCCAACTCCTTCATTCACGCGCTTGTCGCGGCGGATTTCGATCCAACCCACGACGTCTTCGGATGCTCTCGGAATCGGGTGTCTACGGAGTTCCGGTACCGGTGCTGCTTTCTGTGGGTGGACGTGTCGTTTCCGGTTCTGAAACGGTACTTGTCGAAGCGGGTTGACGAGATGTTAGAGGCCGGGATGGTAAGGGAGTTGGCGGAGTTTTACGACCCGGAAATTGCCGATTCAGAGACCCGAGTGGGGCTGAGGAAGGCGATCGGAGTGGCGGAATTCGAGGAGTTTTTCCGGCGGCATCACCCGAAAGGGAGAGAGTACAAAGAGGGGAGGGATCCAGTGGTGGCGAGCGCATACAAAGAGGCGGTAAGGAAAATAAAGGAAAACACGTGGAAGCTAACAAAGAGGCAGTTAGGGAAGATCGGACGGCTGAGAAGAGCGGGGTGGGACCTGAAAAGAGTGGACGCGACAGCGGCGGTTGTGGCGGTGCTGGGGTCGGATGCGGCGGAGAAACGGTCAGAAATATGGGAGGCAGAGGTAGTGGAAGCAAGCGTAAAGATTGTGAAGAGGTTCATTGCGTAG

mRNA sequence

ATGACACTTTCCCTCTCCTTCTCCCATTTTAATGCCCCTTTTTCCTGTTCTTCCCGCCGCCCCCGATCCCCACTTGTCCTCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCCCCTCCCCCTCCCATAATCTCATCCTCCTCCTCGGCGCCACCGGCTCCGGCAAGTCCTCCCTCTCCATTGACCTCGCCTCCCGCTTCCCCTCCGAGATTATTAATTCTGACAAAATGCAACTCTACACAGGCCTCGACATCACCACTAACAAGCTCCCCCTCTCCGACCGCCGCGCCGTCCCCCACCATCTTCTCGGCGACTTCGATCCTCTCGCCGGCGAAGTCTCCGCATCAGCATTCCGCTCCCACGCCGCGGCCGTTATCTCCGGCGTAGTCTCCCGAGGGAACCTTCCTCTCGTCGTCGGCGGTTCCAACTCCTTCATTCACGCGCTTGTCGCGGCGGATTTCGATCCAACCCACGACGTCTTCGGATGCTCTCGGAATCGGGTGTCTACGGAGTTCCGGTACCGGTGCTGCTTTCTGTGGGTGGACGTGTCGTTTCCGGTTCTGAAACGGTACTTGTCGAAGCGGGTTGACGAGATGTTAGAGGCCGGGATGGTAAGGGAGTTGGCGGAGTTTTACGACCCGGAAATTGCCGATTCAGAGACCCGAGTGGGGCTGAGGAAGGCGATCGGAGTGGCGGAATTCGAGGAGTTTTTCCGGCGGCATCACCCGAAAGGGAGAGAGTACAAAGAGGGGAGGGATCCAGTGGTGGCGAGCGCATACAAAGAGGCGGTAAGGAAAATAAAGGAAAACACGTGGAAGCTAACAAAGAGGCAGTTAGGGAAGATCGGACGGCTGAGAAGAGCGGGGTGGGACCTGAAAAGAGTGGACGCGACAGCGGCGGTTGTGGCGGTGCTGGGGTCGGATGCGGCGGAGAAACGGTCAGAAATATGGGAGGCAGAGGTAGTGGAAGCAAGCGTAAAGATTGTGAAGAGGTTCATTGCGTAG

Coding sequence (CDS)

ATGACACTTTCCCTCTCCTTCTCCCATTTTAATGCCCCTTTTTCCTGTTCTTCCCGCCGCCCCCGATCCCCACTTGTCCTCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCCCCTCCCCCTCCCATAATCTCATCCTCCTCCTCGGCGCCACCGGCTCCGGCAAGTCCTCCCTCTCCATTGACCTCGCCTCCCGCTTCCCCTCCGAGATTATTAATTCTGACAAAATGCAACTCTACACAGGCCTCGACATCACCACTAACAAGCTCCCCCTCTCCGACCGCCGCGCCGTCCCCCACCATCTTCTCGGCGACTTCGATCCTCTCGCCGGCGAAGTCTCCGCATCAGCATTCCGCTCCCACGCCGCGGCCGTTATCTCCGGCGTAGTCTCCCGAGGGAACCTTCCTCTCGTCGTCGGCGGTTCCAACTCCTTCATTCACGCGCTTGTCGCGGCGGATTTCGATCCAACCCACGACGTCTTCGGATGCTCTCGGAATCGGGTGTCTACGGAGTTCCGGTACCGGTGCTGCTTTCTGTGGGTGGACGTGTCGTTTCCGGTTCTGAAACGGTACTTGTCGAAGCGGGTTGACGAGATGTTAGAGGCCGGGATGGTAAGGGAGTTGGCGGAGTTTTACGACCCGGAAATTGCCGATTCAGAGACCCGAGTGGGGCTGAGGAAGGCGATCGGAGTGGCGGAATTCGAGGAGTTTTTCCGGCGGCATCACCCGAAAGGGAGAGAGTACAAAGAGGGGAGGGATCCAGTGGTGGCGAGCGCATACAAAGAGGCGGTAAGGAAAATAAAGGAAAACACGTGGAAGCTAACAAAGAGGCAGTTAGGGAAGATCGGACGGCTGAGAAGAGCGGGGTGGGACCTGAAAAGAGTGGACGCGACAGCGGCGGTTGTGGCGGTGCTGGGGTCGGATGCGGCGGAGAAACGGTCAGAAATATGGGAGGCAGAGGTAGTGGAAGCAAGCGTAAAGATTGTGAAGAGGTTCATTGCGTAG

Protein sequence

MTLSLSFSHFNAPFSCSSRRPRSPLVLSSSSSSSSSSSSPSPSHNLILLLGATGSGKSSLSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFRSHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPTHDVFGCSRNRVSTEFRYRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATAAVVAVLGSDAAEKRSEIWEAEVVEASVKIVKRFIA
Homology
BLAST of Cp4.1LG13g06780 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 1.7e-84
Identity = 173/296 (58.45%), Postives = 214/296 (72.30%), Query Frame = 0

Query: 46  LILLLGATGSGKSSLSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLG 105
           L++L+GATG+GKS LSIDLA+ FP E+INSDKMQ+Y GLDITTNK+ + DR  VPHHLLG
Sbjct: 32  LLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTNKISVPDRGGVPHHLLG 91

Query: 106 DFDPLAGEVSASAFRSHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPT-HDVF-G 165
           + DP  GE++ + FRS A   +S +  R  LP++VGGSNSFIHAL+   FD +   VF  
Sbjct: 92  EVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHALLVDRFDSSGPGVFEE 151

Query: 166 CSRNRVSTEFRYRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPE----IAD 225
            S + VS+E RY CCFLWVDVS  VL  YL+KRVD+MLE GM  ELAEFY PE      D
Sbjct: 152 GSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMFDELAEFYSPEDEDHDED 211

Query: 226 SETRVGLRKAIGVAEFEEFFRRHHPKGREYKE-GRDPVVASAYKEAVRKIKENTWKLTKR 285
           S TR GLRKAIGV EF+ +F +  P   E ++ GRD V   A++EAVR IKENT  L KR
Sbjct: 212 SATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRRGAFEEAVRAIKENTCHLAKR 271

Query: 286 QLGKIGRLRRAGWDLKRVDATAAVVAVLGSDAAEKRSEIWEAEVVEASVKIVKRFI 335
           Q+GKI RL+ AGWDL+R+DAT +  A + SD+ EK +EIWE +V+E SVKIV RF+
Sbjct: 272 QIGKILRLKGAGWDLRRLDATESFRAAMTSDSGEKCTEIWEKQVLEPSVKIVSRFL 327

BLAST of Cp4.1LG13g06780 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 302.4 bits (773), Expect = 6.5e-81
Identity = 170/339 (50.15%), Postives = 229/339 (67.55%), Query Frame = 0

Query: 2   TLSLSFSHFNAPFS-----CSSRRPRSPLVLSSSSSSSSSSSSPSPSHNLILLLGATGSG 61
           T S SFS  ++  S        RR   PLV   SS     S S +    ++++LGATG+G
Sbjct: 21  TTSPSFSSHSSSSSSLLSFTKRRRKHQPLV---SSIRMEQSRSRNRKDKVVVILGATGAG 80

Query: 62  KSSLSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSA 121
           KS LS+DLA+RFPSEIINSDK+Q+Y GL+ITTN++ L DRR VPHHLLG  +P  GE++A
Sbjct: 81  KSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTA 140

Query: 122 SAFRSHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPTHDVFGCSRNRVSTEFRYR 181
             FRS A+ V+  + SR  +P++ GGSNSF+HAL+A  FDP  D F      +S++ RY 
Sbjct: 141 GEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYE 200

Query: 182 CCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFE 241
           CCF+WVDVS  VL  YL +RVDEM+++GM  EL+ FYDP  +  ETR G+RKAIGV EF+
Sbjct: 201 CCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFD 260

Query: 242 EFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRV 301
            +F+ + P+ +  K   D +  +AY +AV  IK NTW L KRQ+ KI  L+ AGW+++RV
Sbjct: 261 GYFKEYPPEKKMIK--WDALRKAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDAGWEIERV 320

Query: 302 DATAAVVAVLGSDAAEKR-SEIWEAEVVEASVKIVKRFI 335
           DATA+  AV+   ++EK+  E WE +V+E SVKIVKR +
Sbjct: 321 DATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHL 354

BLAST of Cp4.1LG13g06780 vs. ExPASy Swiss-Prot
Match: Q9LJL4 (Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT8 PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.3e-73
Identity = 143/295 (48.47%), Postives = 198/295 (67.12%), Query Frame = 0

Query: 41  SPSHNLILLLGATGSGKSSLSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVP 100
           S    +++++GATGSGKS LSIDLA+RF  EI+NSDK+Q Y GL +TTN++ + +R  VP
Sbjct: 40  SYKQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVP 99

Query: 101 HHLLGDFDPLAGEVSASAFRSHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPTHD 160
           HHLLG+  P   E++ S FRS A+  IS + +RGNLP++ GGSNSFIHAL+   FDP   
Sbjct: 100 HHLLGELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTY 159

Query: 161 VFGCSRNRVSTEFRYRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADS 220
            F  S   +S+  RY CCFLWVDVS  VL  YLSKRVD+M+E+GM  ELA FYDP  + S
Sbjct: 160 PFS-SETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGS 219

Query: 221 ETRV-GLRKAIGVAEFEEFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQ 280
             R  G+ K IG+ EF+ +F  + P+ ++     D     AY EAV++IKENTW+L K+Q
Sbjct: 220 AIRAHGIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAVQEIKENTWRLAKKQ 279

Query: 281 LGKIGRLRRAGWDLKRVDATAAVVAVLGSDAAEKRSEIWEAEVVEASVKIVKRFI 335
           + +I +L+ +GWD++R+DAT +              EIW+  V++ S+K+VKRF+
Sbjct: 280 IERIMKLKSSGWDIQRLDATPSF--------GRSSREIWDNTVLDESIKVVKRFL 325

BLAST of Cp4.1LG13g06780 vs. ExPASy Swiss-Prot
Match: Q9C6L1 (Adenylate isopentenyltransferase 6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT6 PE=2 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 2.5e-72
Identity = 150/296 (50.68%), Postives = 206/296 (69.59%), Query Frame = 0

Query: 46  LILLLGATGSGKSSLSIDLASR-FPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLL 105
           ++L+ G TG+GKS LS+DLA+R FP+EIINSDKMQ+Y G +I TN +PL ++  VPHHLL
Sbjct: 47  VVLITGTTGTGKSRLSVDLATRFFPAEIINSDKMQIYKGFEIVTNLIPLHEQGGVPHHLL 106

Query: 106 GDFDPLAGEVSASAFRSHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPTHDVF-- 165
           G F P  GE++ + FRS A   IS ++S   LP+VVGGSNSF HAL+A  FDP  D F  
Sbjct: 107 GQFHPQDGELTPAEFRSLATLSISKLISSKKLPIVVGGSNSFNHALLAERFDPDIDPFSP 166

Query: 166 GCSRNRVSTEFRYRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSET 225
           G S + + ++ RY+CC LWVDV  PVL ++L  RVD+M+E+G+V +LAE YDP + DS  
Sbjct: 167 GSSLSTICSDLRYKCCILWVDVLEPVLFQHLCNRVDQMIESGLVEQLAELYDP-VVDSGR 226

Query: 226 RVGLRKAIGVAEFEEFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGK 285
           R+G+RK IGV EF+ +F R +PK  + K   D    +AY+E V+ +KE T +L K+Q  K
Sbjct: 227 RLGVRKTIGVEEFDRYF-RVYPKEMD-KGIWDLARKAAYEETVKGMKERTCRLVKKQKEK 286

Query: 286 IGRLRRAGWDLKRVDATAAVVAVLGSDAAEKRS----EIWEAEVVEASVKIVKRFI 335
           I +L R GW++KR+DATAA++A L    A+       EIWE  +V+ SV+IVK+F+
Sbjct: 287 IMKLIRGGWEIKRLDATAAIMAELNQSTAKGEGKNGREIWEKHIVDESVEIVKKFL 339

BLAST of Cp4.1LG13g06780 vs. ExPASy Swiss-Prot
Match: Q9SB60 (Adenylate isopentenyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=IPT4 PE=1 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 4.2e-72
Identity = 145/291 (49.83%), Postives = 200/291 (68.73%), Query Frame = 0

Query: 46  LILLLGATGSGKSSLSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLG 105
           +++++GATGSGKSSLS+DLA  F +EIINSDKMQ Y GL ITTN+  + DRR VPHHLLG
Sbjct: 7   MVVIMGATGSGKSSLSVDLALHFKAEIINSDKMQFYDGLKITTNQSTIEDRRGVPHHLLG 66

Query: 106 DFDPLAGEVSASAFRSHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPTHDVFGCS 165
           + +P AGEV+A+ FR  AA  IS +  R  LP++ GGSNS+IHAL+A  +DP +  F   
Sbjct: 67  ELNPEAGEVTAAEFRVMAAEAISEITQRKKLPILAGGSNSYIHALLAKSYDPENYPFSDH 126

Query: 166 RNRVSTEFRYRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVG 225
           +  + +E +Y CCF+W+DV   VL  YLS R+D M+++GM  E+AEF+  + A  E  +G
Sbjct: 127 KGSICSELKYDCCFIWIDVDQSVLFEYLSLRLDLMMKSGMFEEIAEFHRSKKAPKEP-LG 186

Query: 226 LRKAIGVAEFEEFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGR 285
           + KAIGV EF+++ + +  K     +  DP+   AY++AVR IKENT++LTK Q+ KI +
Sbjct: 187 IWKAIGVQEFDDYLKMY--KWDNDMDKWDPMRKEAYEKAVRAIKENTFQLTKDQITKINK 246

Query: 286 LRRAGWDLKRVDATAAV-----VAVLGSDAAEKRSEIWEAEVVEASVKIVK 332
           LR AGWD+K+VDATA+       A  G   AE + +IW  EV+E  VKIV+
Sbjct: 247 LRNAGWDIKKVDATASFREAIRAAKEGEGVAEMQRKIWNKEVLEPCVKIVR 294

BLAST of Cp4.1LG13g06780 vs. NCBI nr
Match: XP_023551170.1 (adenylate isopentenyltransferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 643 bits (1659), Expect = 1.85e-231
Identity = 335/335 (100.00%), Postives = 335/335 (100.00%), Query Frame = 0

Query: 1   MTLSLSFSHFNAPFSCSSRRPRSPLVLSSSSSSSSSSSSPSPSHNLILLLGATGSGKSSL 60
           MTLSLSFSHFNAPFSCSSRRPRSPLVLSSSSSSSSSSSSPSPSHNLILLLGATGSGKSSL
Sbjct: 52  MTLSLSFSHFNAPFSCSSRRPRSPLVLSSSSSSSSSSSSPSPSHNLILLLGATGSGKSSL 111

Query: 61  SIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFR 120
           SIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFR
Sbjct: 112 SIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFR 171

Query: 121 SHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPTHDVFGCSRNRVSTEFRYRCCFL 180
           SHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPTHDVFGCSRNRVSTEFRYRCCFL
Sbjct: 172 SHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPTHDVFGCSRNRVSTEFRYRCCFL 231

Query: 181 WVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFR 240
           WVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFR
Sbjct: 232 WVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFR 291

Query: 241 RHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATA 300
           RHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATA
Sbjct: 292 RHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATA 351

Query: 301 AVVAVLGSDAAEKRSEIWEAEVVEASVKIVKRFIA 335
           AVVAVLGSDAAEKRSEIWEAEVVEASVKIVKRFIA
Sbjct: 352 AVVAVLGSDAAEKRSEIWEAEVVEASVKIVKRFIA 386

BLAST of Cp4.1LG13g06780 vs. NCBI nr
Match: KAG6579205.1 (hypothetical protein SDJN03_23653, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016719.1 hypothetical protein SDJN02_21829, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 617 bits (1590), Expect = 6.53e-222
Identity = 320/335 (95.52%), Postives = 324/335 (96.72%), Query Frame = 0

Query: 1   MTLSLSFSHFNAPFSCSSRRPRSPLVLSSSSSSSSSSSSPSPSHNLILLLGATGSGKSSL 60
           MTLSLSFSHFNAPFSCSSRRPRSPLVLSSSS          PSHNLILLLGATGSGKSSL
Sbjct: 1   MTLSLSFSHFNAPFSCSSRRPRSPLVLSSSS----------PSHNLILLLGATGSGKSSL 60

Query: 61  SIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFR 120
           SIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFR
Sbjct: 61  SIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFR 120

Query: 121 SHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPTHDVFGCSRNRVSTEFRYRCCFL 180
           SHAAAVISGV+SRGNLPLVVGGSNSFIHALV ADFDP+HDVFGCSRNRVSTEFRYRCCFL
Sbjct: 121 SHAAAVISGVISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCFL 180

Query: 181 WVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFR 240
           WVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFR
Sbjct: 181 WVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFR 240

Query: 241 RHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATA 300
           RHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATA
Sbjct: 241 RHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATA 300

Query: 301 AVVAVLGSDAAEKRSEIWEAEVVEASVKIVKRFIA 335
           AVVA+LGS+AAEKRSEIWEAEVVEASVKIVKRFIA
Sbjct: 301 AVVAMLGSEAAEKRSEIWEAEVVEASVKIVKRFIA 325

BLAST of Cp4.1LG13g06780 vs. NCBI nr
Match: XP_022939170.1 (adenylate isopentenyltransferase-like [Cucurbita moschata])

HSP 1 Score: 611 bits (1576), Expect = 5.07e-219
Identity = 321/341 (94.13%), Postives = 324/341 (95.01%), Query Frame = 0

Query: 1   MTLSLSFSH------FNAPFSCSSRRPRSPLVLSSSSSSSSSSSSPSPSHNLILLLGATG 60
           MTLSLSFSH      FNAPFSCSSRRPRSPLVLSSSS          PSHNLILLLGATG
Sbjct: 43  MTLSLSFSHSSLHSHFNAPFSCSSRRPRSPLVLSSSS----------PSHNLILLLGATG 102

Query: 61  SGKSSLSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEV 120
           SGKSSLSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEV
Sbjct: 103 SGKSSLSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEV 162

Query: 121 SASAFRSHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPTHDVFGCSRNRVSTEFR 180
           SASAFRSHAAAVISGV+SRGNLPLVVGGSNSFIHALV ADFDP+HDVFGCSRNRVSTEFR
Sbjct: 163 SASAFRSHAAAVISGVISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFR 222

Query: 181 YRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAE 240
           YRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAE
Sbjct: 223 YRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAE 282

Query: 241 FEEFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLK 300
           FEEFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLK
Sbjct: 283 FEEFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLK 342

Query: 301 RVDATAAVVAVLGSDAAEKRSEIWEAEVVEASVKIVKRFIA 335
           RVDATAAVVAVLGS+AAEKRSEIWEAEVVEASVKIVKRFIA
Sbjct: 343 RVDATAAVVAVLGSEAAEKRSEIWEAEVVEASVKIVKRFIA 373

BLAST of Cp4.1LG13g06780 vs. NCBI nr
Match: XP_008445640.1 (PREDICTED: adenylate isopentenyltransferase-like [Cucumis melo] >KAA0049650.1 adenylate isopentenyltransferase-like [Cucumis melo var. makuwa] >TYK08909.1 adenylate isopentenyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 512 bits (1318), Expect = 1.43e-180
Identity = 273/334 (81.74%), Postives = 295/334 (88.32%), Query Frame = 0

Query: 1   MTLSLSFSHFNAPFSCSSRRPRSPLVLSSSSSSSSSSSSPSPSHNLILLLGATGSGKSSL 60
           MTLSL FSH + P S   RRPR PLVLSS+           PS +L+LLLGATGSGKSSL
Sbjct: 1   MTLSLPFSH-SFPLS---RRPRCPLVLSSA-----------PSDSLLLLLGATGSGKSSL 60

Query: 61  SIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFR 120
           SIDLA+RFPSEIINSDKMQLY GLDITTNKLPLS RRAVPHHLLGD+DPLAGEVS +AFR
Sbjct: 61  SIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDYDPLAGEVSPAAFR 120

Query: 121 SHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPTHDVFGCSRNRVSTEFRYRCCFL 180
           SHA+AVISGV+SRGNLPLVVGGSNSFIHALVAADFD T DVF  SRNRVS EFRYRCCFL
Sbjct: 121 SHASAVISGVISRGNLPLVVGGSNSFIHALVAADFDDTQDVFCGSRNRVSAEFRYRCCFL 180

Query: 181 WVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFR 240
           WVDVSFPVLKRYLSKRVDEML+ GM+ ELA+FYDPE+AD ETRVGLRKAIGV EFEE+FR
Sbjct: 181 WVDVSFPVLKRYLSKRVDEMLDGGMLEELAQFYDPEMADLETRVGLRKAIGVPEFEEYFR 240

Query: 241 RHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATA 300
           RHHPKGR+Y+EG DP+ ASA++EAVRKIKENTW+LTKRQL KIGRLRRAGWDLKRVDATA
Sbjct: 241 RHHPKGRDYREG-DPLQASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATA 300

Query: 301 AVVAVLGSDAAEKRSEIWEAEVVEASVKIVKRFI 334
           AVVAVL S+AAEKRSEIWE +VVE SVKIVK FI
Sbjct: 301 AVVAVLASEAAEKRSEIWETQVVEPSVKIVKGFI 318

BLAST of Cp4.1LG13g06780 vs. NCBI nr
Match: XP_004150914.1 (adenylate isopentenyltransferase [Cucumis sativus] >KGN45943.1 hypothetical protein Csa_005709 [Cucumis sativus])

HSP 1 Score: 509 bits (1311), Expect = 1.66e-179
Identity = 272/334 (81.44%), Postives = 293/334 (87.72%), Query Frame = 0

Query: 1   MTLSLSFSHFNAPFSCSSRRPRSPLVLSSSSSSSSSSSSPSPSHNLILLLGATGSGKSSL 60
           MTLSL FSH    F  S RRPR PL+LSS+           PS +L+LLLGATGSGKSSL
Sbjct: 1   MTLSLPFSH---SFLLS-RRPRCPLILSSA-----------PSDSLLLLLGATGSGKSSL 60

Query: 61  SIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFR 120
           SIDLA+RFPSEIINSDKMQLY GLDITTNKLPLS RRAVPHHLLGDFDPLAGEVS +AFR
Sbjct: 61  SIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFR 120

Query: 121 SHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPTHDVFGCSRNRVSTEFRYRCCFL 180
           SHA+AVISGV+SRGNLPLVVGGSNSF+HALVAADFD T DVF  SRNRVSTEF YRCCFL
Sbjct: 121 SHASAVISGVISRGNLPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFL 180

Query: 181 WVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFR 240
           WVDVSFPVLKRYLSKRVDEML+ GMV ELA+FYDPE+ADSETRVGLRKAIGV EFEE+FR
Sbjct: 181 WVDVSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFR 240

Query: 241 RHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATA 300
           RHHPKGR+Y+EG DP+ A A++EAVRKIKENTW+LTKRQL KIGRLRRAGWDLKRVDATA
Sbjct: 241 RHHPKGRDYREG-DPLQARAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATA 300

Query: 301 AVVAVLGSDAAEKRSEIWEAEVVEASVKIVKRFI 334
           AVVAVL S+ AEKRSEIWE +VVE SVKIVK FI
Sbjct: 301 AVVAVLASEVAEKRSEIWETQVVEPSVKIVKGFI 318

BLAST of Cp4.1LG13g06780 vs. ExPASy TrEMBL
Match: A0A6J1FLX4 (adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111445165 PE=4 SV=1)

HSP 1 Score: 611 bits (1576), Expect = 2.45e-219
Identity = 321/341 (94.13%), Postives = 324/341 (95.01%), Query Frame = 0

Query: 1   MTLSLSFSH------FNAPFSCSSRRPRSPLVLSSSSSSSSSSSSPSPSHNLILLLGATG 60
           MTLSLSFSH      FNAPFSCSSRRPRSPLVLSSSS          PSHNLILLLGATG
Sbjct: 43  MTLSLSFSHSSLHSHFNAPFSCSSRRPRSPLVLSSSS----------PSHNLILLLGATG 102

Query: 61  SGKSSLSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEV 120
           SGKSSLSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEV
Sbjct: 103 SGKSSLSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEV 162

Query: 121 SASAFRSHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPTHDVFGCSRNRVSTEFR 180
           SASAFRSHAAAVISGV+SRGNLPLVVGGSNSFIHALV ADFDP+HDVFGCSRNRVSTEFR
Sbjct: 163 SASAFRSHAAAVISGVISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFR 222

Query: 181 YRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAE 240
           YRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAE
Sbjct: 223 YRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAE 282

Query: 241 FEEFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLK 300
           FEEFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLK
Sbjct: 283 FEEFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLK 342

Query: 301 RVDATAAVVAVLGSDAAEKRSEIWEAEVVEASVKIVKRFIA 335
           RVDATAAVVAVLGS+AAEKRSEIWEAEVVEASVKIVKRFIA
Sbjct: 343 RVDATAAVVAVLGSEAAEKRSEIWEAEVVEASVKIVKRFIA 373

BLAST of Cp4.1LG13g06780 vs. ExPASy TrEMBL
Match: A0A5D3CF13 (Adenylate isopentenyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold383G00180 PE=4 SV=1)

HSP 1 Score: 512 bits (1318), Expect = 6.90e-181
Identity = 273/334 (81.74%), Postives = 295/334 (88.32%), Query Frame = 0

Query: 1   MTLSLSFSHFNAPFSCSSRRPRSPLVLSSSSSSSSSSSSPSPSHNLILLLGATGSGKSSL 60
           MTLSL FSH + P S   RRPR PLVLSS+           PS +L+LLLGATGSGKSSL
Sbjct: 1   MTLSLPFSH-SFPLS---RRPRCPLVLSSA-----------PSDSLLLLLGATGSGKSSL 60

Query: 61  SIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFR 120
           SIDLA+RFPSEIINSDKMQLY GLDITTNKLPLS RRAVPHHLLGD+DPLAGEVS +AFR
Sbjct: 61  SIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDYDPLAGEVSPAAFR 120

Query: 121 SHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPTHDVFGCSRNRVSTEFRYRCCFL 180
           SHA+AVISGV+SRGNLPLVVGGSNSFIHALVAADFD T DVF  SRNRVS EFRYRCCFL
Sbjct: 121 SHASAVISGVISRGNLPLVVGGSNSFIHALVAADFDDTQDVFCGSRNRVSAEFRYRCCFL 180

Query: 181 WVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFR 240
           WVDVSFPVLKRYLSKRVDEML+ GM+ ELA+FYDPE+AD ETRVGLRKAIGV EFEE+FR
Sbjct: 181 WVDVSFPVLKRYLSKRVDEMLDGGMLEELAQFYDPEMADLETRVGLRKAIGVPEFEEYFR 240

Query: 241 RHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATA 300
           RHHPKGR+Y+EG DP+ ASA++EAVRKIKENTW+LTKRQL KIGRLRRAGWDLKRVDATA
Sbjct: 241 RHHPKGRDYREG-DPLQASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATA 300

Query: 301 AVVAVLGSDAAEKRSEIWEAEVVEASVKIVKRFI 334
           AVVAVL S+AAEKRSEIWE +VVE SVKIVK FI
Sbjct: 301 AVVAVLASEAAEKRSEIWETQVVEPSVKIVKGFI 318

BLAST of Cp4.1LG13g06780 vs. ExPASy TrEMBL
Match: A0A1S3BD73 (adenylate isopentenyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103488599 PE=4 SV=1)

HSP 1 Score: 512 bits (1318), Expect = 6.90e-181
Identity = 273/334 (81.74%), Postives = 295/334 (88.32%), Query Frame = 0

Query: 1   MTLSLSFSHFNAPFSCSSRRPRSPLVLSSSSSSSSSSSSPSPSHNLILLLGATGSGKSSL 60
           MTLSL FSH + P S   RRPR PLVLSS+           PS +L+LLLGATGSGKSSL
Sbjct: 1   MTLSLPFSH-SFPLS---RRPRCPLVLSSA-----------PSDSLLLLLGATGSGKSSL 60

Query: 61  SIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFR 120
           SIDLA+RFPSEIINSDKMQLY GLDITTNKLPLS RRAVPHHLLGD+DPLAGEVS +AFR
Sbjct: 61  SIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDYDPLAGEVSPAAFR 120

Query: 121 SHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPTHDVFGCSRNRVSTEFRYRCCFL 180
           SHA+AVISGV+SRGNLPLVVGGSNSFIHALVAADFD T DVF  SRNRVS EFRYRCCFL
Sbjct: 121 SHASAVISGVISRGNLPLVVGGSNSFIHALVAADFDDTQDVFCGSRNRVSAEFRYRCCFL 180

Query: 181 WVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFR 240
           WVDVSFPVLKRYLSKRVDEML+ GM+ ELA+FYDPE+AD ETRVGLRKAIGV EFEE+FR
Sbjct: 181 WVDVSFPVLKRYLSKRVDEMLDGGMLEELAQFYDPEMADLETRVGLRKAIGVPEFEEYFR 240

Query: 241 RHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATA 300
           RHHPKGR+Y+EG DP+ ASA++EAVRKIKENTW+LTKRQL KIGRLRRAGWDLKRVDATA
Sbjct: 241 RHHPKGRDYREG-DPLQASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATA 300

Query: 301 AVVAVLGSDAAEKRSEIWEAEVVEASVKIVKRFI 334
           AVVAVL S+AAEKRSEIWE +VVE SVKIVK FI
Sbjct: 301 AVVAVLASEAAEKRSEIWETQVVEPSVKIVKGFI 318

BLAST of Cp4.1LG13g06780 vs. ExPASy TrEMBL
Match: A0A0A0K943 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G030440 PE=4 SV=1)

HSP 1 Score: 509 bits (1311), Expect = 8.03e-180
Identity = 272/334 (81.44%), Postives = 293/334 (87.72%), Query Frame = 0

Query: 1   MTLSLSFSHFNAPFSCSSRRPRSPLVLSSSSSSSSSSSSPSPSHNLILLLGATGSGKSSL 60
           MTLSL FSH    F  S RRPR PL+LSS+           PS +L+LLLGATGSGKSSL
Sbjct: 1   MTLSLPFSH---SFLLS-RRPRCPLILSSA-----------PSDSLLLLLGATGSGKSSL 60

Query: 61  SIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFR 120
           SIDLA+RFPSEIINSDKMQLY GLDITTNKLPLS RRAVPHHLLGDFDPLAGEVS +AFR
Sbjct: 61  SIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFR 120

Query: 121 SHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPTHDVFGCSRNRVSTEFRYRCCFL 180
           SHA+AVISGV+SRGNLPLVVGGSNSF+HALVAADFD T DVF  SRNRVSTEF YRCCFL
Sbjct: 121 SHASAVISGVISRGNLPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFL 180

Query: 181 WVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFR 240
           WVDVSFPVLKRYLSKRVDEML+ GMV ELA+FYDPE+ADSETRVGLRKAIGV EFEE+FR
Sbjct: 181 WVDVSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFR 240

Query: 241 RHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATA 300
           RHHPKGR+Y+EG DP+ A A++EAVRKIKENTW+LTKRQL KIGRLRRAGWDLKRVDATA
Sbjct: 241 RHHPKGRDYREG-DPLQARAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATA 300

Query: 301 AVVAVLGSDAAEKRSEIWEAEVVEASVKIVKRFI 334
           AVVAVL S+ AEKRSEIWE +VVE SVKIVK FI
Sbjct: 301 AVVAVLASEVAEKRSEIWETQVVEPSVKIVKGFI 318

BLAST of Cp4.1LG13g06780 vs. ExPASy TrEMBL
Match: A0A6J1HF40 (adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111463593 PE=4 SV=1)

HSP 1 Score: 491 bits (1263), Expect = 2.70e-172
Identity = 265/344 (77.03%), Postives = 289/344 (84.01%), Query Frame = 0

Query: 1   MTLSLSFSHFNAP----------FSCSSRRPRSPLVLSSSSSSSSSSSSPSPSHNLILLL 60
           MTLSL FSH +            F   SRR + P +LSS            PS NL+LLL
Sbjct: 1   MTLSLPFSHSSLHYHHHHHPFPLFLSPSRRFQWPRMLSSL-----------PSDNLLLLL 60

Query: 61  GATGSGKSSLSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPL 120
           GATGSGKSSLSID+A+RFPSEIINSDKMQLY GLD+TTNKLP SDRR VPHHLLGDFDPL
Sbjct: 61  GATGSGKSSLSIDIAARFPSEIINSDKMQLYRGLDVTTNKLPFSDRRGVPHHLLGDFDPL 120

Query: 121 AGEVSASAFRSHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPTHDVFGCSRNRVS 180
           AGEVSA+AFRS A+AVISGVVSRGNLP+VVGGSNS+IHALV A FD  +DVF    NRVS
Sbjct: 121 AGEVSAAAFRSQASAVISGVVSRGNLPVVVGGSNSYIHALVTAGFDQDYDVFDGPLNRVS 180

Query: 181 TEFRYRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAI 240
            EFRYRCCFLWVDVSFPVLK+YLSKRVD+MLEAGMV ELAEFYDPE+A++E RVGLRKAI
Sbjct: 181 AEFRYRCCFLWVDVSFPVLKQYLSKRVDDMLEAGMVGELAEFYDPEMAETEPRVGLRKAI 240

Query: 241 GVAEFEEFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAG 300
           GV EFEEFFRR+HPKGREY+EG DP+ A AY+EAVRKIKENTW+LTKRQLGKIGRLR AG
Sbjct: 241 GVPEFEEFFRRYHPKGREYREG-DPLRAGAYEEAVRKIKENTWQLTKRQLGKIGRLRMAG 300

Query: 301 WDLKRVDATAAVVAVLGSDAAEKRSEIWEAEVVEASVKIVKRFI 334
           WDLKRVDATAAVVAVL S+AAEKRSEIWEA+VVE SVKIVKRFI
Sbjct: 301 WDLKRVDATAAVVAVLASEAAEKRSEIWEAQVVEPSVKIVKRFI 332

BLAST of Cp4.1LG13g06780 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 302.4 bits (773), Expect = 4.6e-82
Identity = 170/339 (50.15%), Postives = 229/339 (67.55%), Query Frame = 0

Query: 2   TLSLSFSHFNAPFS-----CSSRRPRSPLVLSSSSSSSSSSSSPSPSHNLILLLGATGSG 61
           T S SFS  ++  S        RR   PLV   SS     S S +    ++++LGATG+G
Sbjct: 21  TTSPSFSSHSSSSSSLLSFTKRRRKHQPLV---SSIRMEQSRSRNRKDKVVVILGATGAG 80

Query: 62  KSSLSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSA 121
           KS LS+DLA+RFPSEIINSDK+Q+Y GL+ITTN++ L DRR VPHHLLG  +P  GE++A
Sbjct: 81  KSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTA 140

Query: 122 SAFRSHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPTHDVFGCSRNRVSTEFRYR 181
             FRS A+ V+  + SR  +P++ GGSNSF+HAL+A  FDP  D F      +S++ RY 
Sbjct: 141 GEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYE 200

Query: 182 CCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFE 241
           CCF+WVDVS  VL  YL +RVDEM+++GM  EL+ FYDP  +  ETR G+RKAIGV EF+
Sbjct: 201 CCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFD 260

Query: 242 EFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRV 301
            +F+ + P+ +  K   D +  +AY +AV  IK NTW L KRQ+ KI  L+ AGW+++RV
Sbjct: 261 GYFKEYPPEKKMIK--WDALRKAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDAGWEIERV 320

Query: 302 DATAAVVAVLGSDAAEKR-SEIWEAEVVEASVKIVKRFI 335
           DATA+  AV+   ++EK+  E WE +V+E SVKIVKR +
Sbjct: 321 DATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHL 354

BLAST of Cp4.1LG13g06780 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 278.1 bits (710), Expect = 9.3e-75
Identity = 143/295 (48.47%), Postives = 198/295 (67.12%), Query Frame = 0

Query: 41  SPSHNLILLLGATGSGKSSLSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVP 100
           S    +++++GATGSGKS LSIDLA+RF  EI+NSDK+Q Y GL +TTN++ + +R  VP
Sbjct: 40  SYKQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVP 99

Query: 101 HHLLGDFDPLAGEVSASAFRSHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPTHD 160
           HHLLG+  P   E++ S FRS A+  IS + +RGNLP++ GGSNSFIHAL+   FDP   
Sbjct: 100 HHLLGELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTY 159

Query: 161 VFGCSRNRVSTEFRYRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADS 220
            F  S   +S+  RY CCFLWVDVS  VL  YLSKRVD+M+E+GM  ELA FYDP  + S
Sbjct: 160 PFS-SETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGS 219

Query: 221 ETRV-GLRKAIGVAEFEEFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQ 280
             R  G+ K IG+ EF+ +F  + P+ ++     D     AY EAV++IKENTW+L K+Q
Sbjct: 220 AIRAHGIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAVQEIKENTWRLAKKQ 279

Query: 281 LGKIGRLRRAGWDLKRVDATAAVVAVLGSDAAEKRSEIWEAEVVEASVKIVKRFI 335
           + +I +L+ +GWD++R+DAT +              EIW+  V++ S+K+VKRF+
Sbjct: 280 IERIMKLKSSGWDIQRLDATPSF--------GRSSREIWDNTVLDESIKVVKRFL 325

BLAST of Cp4.1LG13g06780 vs. TAIR 10
Match: AT1G25410.1 (isopentenyltransferase 6 )

HSP 1 Score: 273.9 bits (699), Expect = 1.8e-73
Identity = 150/296 (50.68%), Postives = 206/296 (69.59%), Query Frame = 0

Query: 46  LILLLGATGSGKSSLSIDLASR-FPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLL 105
           ++L+ G TG+GKS LS+DLA+R FP+EIINSDKMQ+Y G +I TN +PL ++  VPHHLL
Sbjct: 47  VVLITGTTGTGKSRLSVDLATRFFPAEIINSDKMQIYKGFEIVTNLIPLHEQGGVPHHLL 106

Query: 106 GDFDPLAGEVSASAFRSHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPTHDVF-- 165
           G F P  GE++ + FRS A   IS ++S   LP+VVGGSNSF HAL+A  FDP  D F  
Sbjct: 107 GQFHPQDGELTPAEFRSLATLSISKLISSKKLPIVVGGSNSFNHALLAERFDPDIDPFSP 166

Query: 166 GCSRNRVSTEFRYRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSET 225
           G S + + ++ RY+CC LWVDV  PVL ++L  RVD+M+E+G+V +LAE YDP + DS  
Sbjct: 167 GSSLSTICSDLRYKCCILWVDVLEPVLFQHLCNRVDQMIESGLVEQLAELYDP-VVDSGR 226

Query: 226 RVGLRKAIGVAEFEEFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGK 285
           R+G+RK IGV EF+ +F R +PK  + K   D    +AY+E V+ +KE T +L K+Q  K
Sbjct: 227 RLGVRKTIGVEEFDRYF-RVYPKEMD-KGIWDLARKAAYEETVKGMKERTCRLVKKQKEK 286

Query: 286 IGRLRRAGWDLKRVDATAAVVAVLGSDAAEKRS----EIWEAEVVEASVKIVKRFI 335
           I +L R GW++KR+DATAA++A L    A+       EIWE  +V+ SV+IVK+F+
Sbjct: 287 IMKLIRGGWEIKRLDATAAIMAELNQSTAKGEGKNGREIWEKHIVDESVEIVKKFL 339

BLAST of Cp4.1LG13g06780 vs. TAIR 10
Match: AT4G24650.1 (isopentenyltransferase 4 )

HSP 1 Score: 273.1 bits (697), Expect = 3.0e-73
Identity = 145/291 (49.83%), Postives = 200/291 (68.73%), Query Frame = 0

Query: 46  LILLLGATGSGKSSLSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLG 105
           +++++GATGSGKSSLS+DLA  F +EIINSDKMQ Y GL ITTN+  + DRR VPHHLLG
Sbjct: 7   MVVIMGATGSGKSSLSVDLALHFKAEIINSDKMQFYDGLKITTNQSTIEDRRGVPHHLLG 66

Query: 106 DFDPLAGEVSASAFRSHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPTHDVFGCS 165
           + +P AGEV+A+ FR  AA  IS +  R  LP++ GGSNS+IHAL+A  +DP +  F   
Sbjct: 67  ELNPEAGEVTAAEFRVMAAEAISEITQRKKLPILAGGSNSYIHALLAKSYDPENYPFSDH 126

Query: 166 RNRVSTEFRYRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVG 225
           +  + +E +Y CCF+W+DV   VL  YLS R+D M+++GM  E+AEF+  + A  E  +G
Sbjct: 127 KGSICSELKYDCCFIWIDVDQSVLFEYLSLRLDLMMKSGMFEEIAEFHRSKKAPKEP-LG 186

Query: 226 LRKAIGVAEFEEFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGR 285
           + KAIGV EF+++ + +  K     +  DP+   AY++AVR IKENT++LTK Q+ KI +
Sbjct: 187 IWKAIGVQEFDDYLKMY--KWDNDMDKWDPMRKEAYEKAVRAIKENTFQLTKDQITKINK 246

Query: 286 LRRAGWDLKRVDATAAV-----VAVLGSDAAEKRSEIWEAEVVEASVKIVK 332
           LR AGWD+K+VDATA+       A  G   AE + +IW  EV+E  VKIV+
Sbjct: 247 LRNAGWDIKKVDATASFREAIRAAKEGEGVAEMQRKIWNKEVLEPCVKIVR 294

BLAST of Cp4.1LG13g06780 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 201.8 bits (512), Expect = 8.5e-52
Identity = 117/291 (40.21%), Postives = 168/291 (57.73%), Query Frame = 0

Query: 46  LILLLGATGSGKSSLSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLG 105
           ++ ++GATG+GKS L+IDLA+RFP+EI+NSDK+Q+Y GLDI TNK+   +   VPHHLLG
Sbjct: 35  VVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDIVTNKVTPEESLGVPHHLLG 94

Query: 106 DFDPLAGEVSASAFRSHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPTHDVFGCS 165
                  + +A  F+  A   +  +V R  +P++ GGSNS+I ALV              
Sbjct: 95  TVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALV-------------- 154

Query: 166 RNRVSTEFRYRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVG 225
            + V    RY CCFLWVDVS PVL  ++S+RVD+M++ G+V E+   +DP  + S+   G
Sbjct: 155 NDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDP--SSSDYSAG 214

Query: 226 LRKAIGVAEFEEFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGR 285
           +R+AIGV E +EF R       E +           + A+ KIKENT  L  RQL KI R
Sbjct: 215 IRRAIGVPELDEFLR------SEMRNYPAETTERLLETAIEKIKENTCLLACRQLQKIQR 274

Query: 286 L-RRAGWDLKRVDATAAVVAVLGSDAAEKRSEIWEAEVVEASVKIVKRFIA 336
           L ++  W++ RVDAT   +        E+  E W+  V   S   V++F++
Sbjct: 275 LYKQWKWNMHRVDATEVFLR-----RGEEADEAWDNSVAHPSALAVEKFLS 298

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5GHF71.7e-8458.45Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1[more]
Q94ID36.5e-8150.15Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q9LJL41.3e-7348.47Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q9C6L12.5e-7250.68Adenylate isopentenyltransferase 6, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q9SB604.2e-7249.83Adenylate isopentenyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=IPT4 PE=1 ... [more]
Match NameE-valueIdentityDescription
XP_023551170.11.85e-231100.00adenylate isopentenyltransferase-like [Cucurbita pepo subsp. pepo][more]
KAG6579205.16.53e-22295.52hypothetical protein SDJN03_23653, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022939170.15.07e-21994.13adenylate isopentenyltransferase-like [Cucurbita moschata][more]
XP_008445640.11.43e-18081.74PREDICTED: adenylate isopentenyltransferase-like [Cucumis melo] >KAA0049650.1 ad... [more]
XP_004150914.11.66e-17981.44adenylate isopentenyltransferase [Cucumis sativus] >KGN45943.1 hypothetical prot... [more]
Match NameE-valueIdentityDescription
A0A6J1FLX42.45e-21994.13adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111445... [more]
A0A5D3CF136.90e-18181.74Adenylate isopentenyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3BD736.90e-18181.74adenylate isopentenyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103488599 PE... [more]
A0A0A0K9438.03e-18081.44Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G030440 PE=4 SV=1[more]
A0A6J1HF402.70e-17277.03adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111463... [more]
Match NameE-valueIdentityDescription
AT1G68460.14.6e-8250.15isopentenyltransferase 1 [more]
AT3G19160.19.3e-7548.47ATP/ADP isopentenyltransferases [more]
AT1G25410.11.8e-7350.68isopentenyltransferase 6 [more]
AT4G24650.13.0e-7349.83isopentenyltransferase 4 [more]
AT5G19040.18.5e-5240.21isopentenyltransferase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 45..334
e-value: 3.5E-121
score: 405.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 43..220
NoneNo IPR availablePFAMPF01715IPPTcoord: 79..153
e-value: 5.6E-16
score: 59.0
coord: 170..280
e-value: 2.1E-12
score: 47.3
NoneNo IPR availableGENE3D1.10.287.890Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domaincoord: 186..293
e-value: 3.5E-121
score: 405.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 15..42
NoneNo IPR availablePANTHERPTHR11088:SF65ADENYLATE ISOPENTENYLTRANSFERASE 4-RELATEDcoord: 38..334
IPR039657DimethylallyltransferasePANTHERPTHR11088TRNA DIMETHYLALLYLTRANSFERASEcoord: 38..334

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG13g06780.1Cp4.1LG13g06780.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006400 tRNA modification
cellular_component GO:0005739 mitochondrion
molecular_function GO:0009824 AMP dimethylallyltransferase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0052381 tRNA dimethylallyltransferase activity