Cp4.1LG13g03490 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG13g03490
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionPolcalcin Jun o
LocationCp4.1LG13: 3498993 .. 3499565 (+)
RNA-Seq ExpressionCp4.1LG13g03490
SyntenyCp4.1LG13g03490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGATTCAAATCTCTCTTCTCCCTAAAGATCAAGAAATCCGATGCCTCCACCGACTCACCGCTTCGATCTACGGCGGTTCCCCTTCTCGGATCTCGAACGCCGTCTTTCAATTCTCCAGCGCAACTCGCGGAGCTCCAGCAAGTATTCAAGAAATTCGATGTCAACGGCGACGGCAAGATCTGCTCCTCTGAACTCGGATCCATTATGCGGAGCCTCGGCCAGGCGGCCACCGAGGAGGAGCTTCAGAGCATGATCGAGGAGGTGGATGCTGACGGCGACGGCTACATCGACCTCCACGAGTTCATCGAGCTCAACACCAAGGACGTGGATTCCGAGGAGGTGCTGGCGAATCTGAAGGACGCGTTCTCTGTTTACGACATGGACGGAAACGGATCCATTACGGCGGAGGAATTGCAGGAGGTTTTGAAGAGCTTGGGCGACGAATGCTCCTTGGCGGATTGCCGGAAGATGATCACCGGCGTCGACAAAAACGGCGACGGTATGATTTGTTTCGATGAATTCAAAGTGATGATGATGATGAGCGGTTCTCGATCCGGCTTCAATGCTTAA

mRNA sequence

ATGGGATTCAAATCTCTCTTCTCCCTAAAGATCAAGAAATCCGATGCCTCCACCGACTCACCGCTTCGATCTACGGCGGTTCCCCTTCTCGGATCTCGAACGCCGTCTTTCAATTCTCCAGCGCAACTCGCGGAGCTCCAGCAAGTATTCAAGAAATTCGATGTCAACGGCGACGGCAAGATCTGCTCCTCTGAACTCGGATCCATTATGCGGAGCCTCGGCCAGGCGGCCACCGAGGAGGAGCTTCAGAGCATGATCGAGGAGGTGGATGCTGACGGCGACGGCTACATCGACCTCCACGAGTTCATCGAGCTCAACACCAAGGACGTGGATTCCGAGGAGGTGCTGGCGAATCTGAAGGACGCGTTCTCTGTTTACGACATGGACGGAAACGGATCCATTACGGCGGAGGAATTGCAGGAGGTTTTGAAGAGCTTGGGCGACGAATGCTCCTTGGCGGATTGCCGGAAGATGATCACCGGCGTCGACAAAAACGGCGACGGTATGATTTGTTTCGATGAATTCAAAGTGATGATGATGATGAGCGGTTCTCGATCCGGCTTCAATGCTTAA

Coding sequence (CDS)

ATGGGATTCAAATCTCTCTTCTCCCTAAAGATCAAGAAATCCGATGCCTCCACCGACTCACCGCTTCGATCTACGGCGGTTCCCCTTCTCGGATCTCGAACGCCGTCTTTCAATTCTCCAGCGCAACTCGCGGAGCTCCAGCAAGTATTCAAGAAATTCGATGTCAACGGCGACGGCAAGATCTGCTCCTCTGAACTCGGATCCATTATGCGGAGCCTCGGCCAGGCGGCCACCGAGGAGGAGCTTCAGAGCATGATCGAGGAGGTGGATGCTGACGGCGACGGCTACATCGACCTCCACGAGTTCATCGAGCTCAACACCAAGGACGTGGATTCCGAGGAGGTGCTGGCGAATCTGAAGGACGCGTTCTCTGTTTACGACATGGACGGAAACGGATCCATTACGGCGGAGGAATTGCAGGAGGTTTTGAAGAGCTTGGGCGACGAATGCTCCTTGGCGGATTGCCGGAAGATGATCACCGGCGTCGACAAAAACGGCGACGGTATGATTTGTTTCGATGAATTCAAAGTGATGATGATGATGAGCGGTTCTCGATCCGGCTTCAATGCTTAA

Protein sequence

MGFKSLFSLKIKKSDASTDSPLRSTAVPLLGSRTPSFNSPAQLAELQQVFKKFDVNGDGKICSSELGSIMRSLGQAATEEELQSMIEEVDADGDGYIDLHEFIELNTKDVDSEEVLANLKDAFSVYDMDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMICFDEFKVMMMMSGSRSGFNA
Homology
BLAST of Cp4.1LG13g03490 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 1.5e-50
Identity = 98/144 (68.06%), Postives = 116/144 (80.56%), Query Frame = 0

Query: 42  QLAELQQVFKKFDVNGDGKICSSELGSIMRSLGQAATEEELQSMIEEVDADGDGYIDLHE 101
           ++ EL+ VFKKFDVNGDGKI S ELG+IM SLG    EEEL+  I E+D  GDGYI+  E
Sbjct: 34  EIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEE 93

Query: 102 FIELNTKDVDSEEVLANLKDAFSVYDMDGNGSITAEELQEVLKSLGDECSLADCRKMITG 161
           F+ELNTK +D  +VL NLKDAFSVYD+DGNGSI+AEEL EVL+SLGDECS+A+CRKMI G
Sbjct: 94  FVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGG 153

Query: 162 VDKNGDGMICFDEFKVMMMMSGSR 186
           VDK+GDG I F+EFK+MM M   R
Sbjct: 154 VDKDGDGTIDFEEFKIMMTMGSRR 177

BLAST of Cp4.1LG13g03490 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 1.5e-37
Identity = 85/170 (50.00%), Postives = 114/170 (67.06%), Query Frame = 0

Query: 16  ASTDSPLRSTAVPLLGSRTPSFNSPAQLAELQQVFKKFDVNGDGKICSSELGSIMRSLGQ 75
           + + SP    A P  G  +P   +P    E+++VF+KFD NGDG+I  SELG++  SLG 
Sbjct: 14  SGSKSPPLPQADPASGGGSP---APTPEEEMERVFRKFDANGDGRISRSELGALFESLGH 73

Query: 76  AATEEELQSMIEEVDADGDGYIDLHEFIELN-TKDVDSEEVLANLKDAFSVYDMDGNGSI 135
           AAT++EL  M+ E DADGDG+I L EF  LN T   D+  V  +L+ AF V+D DGNG+I
Sbjct: 74  AATDDELARMMAEADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVFDADGNGTI 133

Query: 136 TAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMICFDEFKVMMMMSGS 185
           +A EL  VL  LG++ ++  CR+MI GVD+NGDG+I F+EFKVMM   GS
Sbjct: 134 SAAELARVLHGLGEKATVQQCRRMIEGVDQNGDGLISFEEFKVMMAGGGS 180

BLAST of Cp4.1LG13g03490 vs. ExPASy Swiss-Prot
Match: O64943 (Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2)

HSP 1 Score: 144.4 bits (363), Expect = 1.3e-33
Identity = 71/135 (52.59%), Postives = 97/135 (71.85%), Query Frame = 0

Query: 45  ELQQVFKKFDVNGDGKICSSELGSIMRSLGQAATEEELQSMIEEVDADGDGYIDLHEFIE 104
           EL++VFKKFD NGDGKI  SEL  I+RSLG    E E+++M+EE DADGDGY+ L EF++
Sbjct: 26  ELEEVFKKFDANGDGKISGSELADILRSLGSDVGEAEVKAMMEEADADGDGYVSLQEFVD 85

Query: 105 LNTKDVDSEEVLANLKDAFSVYDMDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDK 164
           LN K       + +LK+AF V+D D NGSI+A EL   L+S+G+ C++ + + +I  VDK
Sbjct: 86  LNNKGAS----VKDLKNAFKVFDRDCNGSISAAELCHTLESVGEPCTIEESKNIIHNVDK 145

Query: 165 NGDGMICFDEFKVMM 180
           NGDG+I  +EF+ MM
Sbjct: 146 NGDGLISVEEFQTMM 156

BLAST of Cp4.1LG13g03490 vs. ExPASy Swiss-Prot
Match: A0T2M3 (Polcalcin Cup a 4 OS=Hesperocyparis arizonica OX=49011 PE=1 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 4.9e-33
Identity = 69/135 (51.11%), Postives = 96/135 (71.11%), Query Frame = 0

Query: 45  ELQQVFKKFDVNGDGKICSSELGSIMRSLGQAATEEELQSMIEEVDADGDGYIDLHEFIE 104
           EL++VFKKFD NGDGKI  SEL  I+RS+G    E E+++M+EE D DGDGY+ L EF++
Sbjct: 26  ELEEVFKKFDANGDGKISGSELADILRSMGSEVDEAEVKAMMEEADTDGDGYVSLQEFVD 85

Query: 105 LNTKDVDSEEVLANLKDAFSVYDMDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDK 164
           LN K       + +LK+AF V+D D NG+I+  EL E LKS+G+ C++ + + +I  VDK
Sbjct: 86  LNIKGA----TVKDLKNAFKVFDRDCNGTISPAELCETLKSVGEPCTIEESKNIIHNVDK 145

Query: 165 NGDGMICFDEFKVMM 180
           NGDG+I  +EF+ MM
Sbjct: 146 NGDGLINVEEFQTMM 156

BLAST of Cp4.1LG13g03490 vs. ExPASy Swiss-Prot
Match: Q6L4D4 (Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica OX=39947 GN=CML15 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.1e-32
Identity = 77/186 (41.40%), Postives = 115/186 (61.83%), Query Frame = 0

Query: 4   KSLFSLKIKKSDASTDSPLRSTAVPLLGSRTPSF---------NSPAQLAELQQVFKKFD 63
           ++ FS K + + +     +R  A+ +  S  PS            PA   E ++VF+KFD
Sbjct: 5   RAFFSRKGRGNSSGRSRSMREAAMNVDWSPRPSDLAAAAAAKPRPPAAEDETERVFRKFD 64

Query: 64  VNGDGKICSSELGSIMRSLGQAATEEELQSMIEEVDADGDGYIDLHEFIELNTKDV-DSE 123
            NGDG+I  +EL ++ RS+G A T++E+  M++E D+DGDGYI L EF  ++     D+ 
Sbjct: 65  ANGDGRISRAELAALFRSVGHAVTDDEVARMMQEADSDGDGYISLGEFAAISAPPPGDAA 124

Query: 124 EVLANLKDAFSVYDMDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMICFD 180
               +L+ AF V+D DGNG IT  EL  VL+ +G+  ++A CR+MI GVD+NGDG+I F+
Sbjct: 125 AAEEDLRHAFGVFDADGNGVITPAELARVLRGIGEAATVAQCRRMIDGVDRNGDGLINFE 184

BLAST of Cp4.1LG13g03490 vs. NCBI nr
Match: XP_023551072.1 (probable calcium-binding protein CML25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 367 bits (942), Expect = 7.82e-128
Identity = 190/190 (100.00%), Postives = 190/190 (100.00%), Query Frame = 0

Query: 1   MGFKSLFSLKIKKSDASTDSPLRSTAVPLLGSRTPSFNSPAQLAELQQVFKKFDVNGDGK 60
           MGFKSLFSLKIKKSDASTDSPLRSTAVPLLGSRTPSFNSPAQLAELQQVFKKFDVNGDGK
Sbjct: 1   MGFKSLFSLKIKKSDASTDSPLRSTAVPLLGSRTPSFNSPAQLAELQQVFKKFDVNGDGK 60

Query: 61  ICSSELGSIMRSLGQAATEEELQSMIEEVDADGDGYIDLHEFIELNTKDVDSEEVLANLK 120
           ICSSELGSIMRSLGQAATEEELQSMIEEVDADGDGYIDLHEFIELNTKDVDSEEVLANLK
Sbjct: 61  ICSSELGSIMRSLGQAATEEELQSMIEEVDADGDGYIDLHEFIELNTKDVDSEEVLANLK 120

Query: 121 DAFSVYDMDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMICFDEFKVMMM 180
           DAFSVYDMDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMICFDEFKVMMM
Sbjct: 121 DAFSVYDMDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMICFDEFKVMMM 180

Query: 181 MSGSRSGFNA 190
           MSGSRSGFNA
Sbjct: 181 MSGSRSGFNA 190

BLAST of Cp4.1LG13g03490 vs. NCBI nr
Match: KAG7029021.1 (putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 347 bits (889), Expect = 9.42e-120
Identity = 180/190 (94.74%), Postives = 185/190 (97.37%), Query Frame = 0

Query: 1   MGFKSLFSLKIKKSDASTDSPLRSTAVPLLGSRTPSFNSPAQLAELQQVFKKFDVNGDGK 60
           MGFKSLFSLKIKKSDASTDSPL STAVP+LGSRTPSFNS AQLAELQQVFKKFDVNGD K
Sbjct: 1   MGFKSLFSLKIKKSDASTDSPLPSTAVPVLGSRTPSFNSRAQLAELQQVFKKFDVNGDAK 60

Query: 61  ICSSELGSIMRSLGQAATEEELQSMIEEVDADGDGYIDLHEFIELNTKDVDSEEVLANLK 120
           ICSSELGSIMRSLGQ ATEEE+QSMIEEVDADGDGYIDLHEFIELNTKDVDS+EVLANLK
Sbjct: 61  ICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLANLK 120

Query: 121 DAFSVYDMDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMICFDEFKVMMM 180
           DAFSVYD+DGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMI FDEFKVMMM
Sbjct: 121 DAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVMMM 180

Query: 181 MSGSRSGFNA 190
           +SGSRSGFNA
Sbjct: 181 VSGSRSGFNA 190

BLAST of Cp4.1LG13g03490 vs. NCBI nr
Match: XP_022939351.1 (probable calcium-binding protein CML25 [Cucurbita moschata])

HSP 1 Score: 346 bits (887), Expect = 1.90e-119
Identity = 179/190 (94.21%), Postives = 185/190 (97.37%), Query Frame = 0

Query: 1   MGFKSLFSLKIKKSDASTDSPLRSTAVPLLGSRTPSFNSPAQLAELQQVFKKFDVNGDGK 60
           MGFKSLFSLKIKKSDASTDSPL ST VP+LGSRTPSFNS AQLA+LQQVFKKFDVNGDGK
Sbjct: 1   MGFKSLFSLKIKKSDASTDSPLPSTPVPVLGSRTPSFNSRAQLADLQQVFKKFDVNGDGK 60

Query: 61  ICSSELGSIMRSLGQAATEEELQSMIEEVDADGDGYIDLHEFIELNTKDVDSEEVLANLK 120
           ICSSELGSIMRSLGQ ATEEE+QSMIEEVDADGDGYIDLHEFIELNTKDVDS+EVLANLK
Sbjct: 61  ICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLANLK 120

Query: 121 DAFSVYDMDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMICFDEFKVMMM 180
           DAFSVYD+DGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMI FDEFKVMMM
Sbjct: 121 DAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVMMM 180

Query: 181 MSGSRSGFNA 190
           +SGSRSGFNA
Sbjct: 181 VSGSRSGFNA 190

BLAST of Cp4.1LG13g03490 vs. NCBI nr
Match: KAG6579273.1 (putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 343 bits (881), Expect = 1.56e-118
Identity = 178/190 (93.68%), Postives = 184/190 (96.84%), Query Frame = 0

Query: 1   MGFKSLFSLKIKKSDASTDSPLRSTAVPLLGSRTPSFNSPAQLAELQQVFKKFDVNGDGK 60
           MGFKSLFSLKIKKSDASTDSPL STAVP+LGSRTPSFNS AQLAELQQVFKKFDVNGD K
Sbjct: 1   MGFKSLFSLKIKKSDASTDSPLPSTAVPVLGSRTPSFNSRAQLAELQQVFKKFDVNGDAK 60

Query: 61  ICSSELGSIMRSLGQAATEEELQSMIEEVDADGDGYIDLHEFIELNTKDVDSEEVLANLK 120
           ICSSELGSIMRSLGQ ATEEE+QSMIEEVDADGDGYIDLHEFIELNTKDVDS+EVLANLK
Sbjct: 61  ICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLANLK 120

Query: 121 DAFSVYDMDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMICFDEFKVMMM 180
           DAFSVYD+DGNGSITAEELQEVLKS+GDECSLADCRKMITGVDKNGDGMI FDEFKVMM 
Sbjct: 121 DAFSVYDIDGNGSITAEELQEVLKSMGDECSLADCRKMITGVDKNGDGMISFDEFKVMMT 180

Query: 181 MSGSRSGFNA 190
           +SGSRSGFNA
Sbjct: 181 VSGSRSGFNA 190

BLAST of Cp4.1LG13g03490 vs. NCBI nr
Match: XP_022973553.1 (probable calcium-binding protein CML25 [Cucurbita maxima])

HSP 1 Score: 340 bits (872), Expect = 3.94e-117
Identity = 179/191 (93.72%), Postives = 184/191 (96.34%), Query Frame = 0

Query: 1   MGFKSLFSLKIKKSDASTDSPLRSTAVPLLGSRTPSFNSPA--QLAELQQVFKKFDVNGD 60
           MGFKSLFSLKIKKSDASTDSPLRSTAVP+LGSRTPSFNS +  QLAELQQVFKKFDVNGD
Sbjct: 1   MGFKSLFSLKIKKSDASTDSPLRSTAVPVLGSRTPSFNSNSREQLAELQQVFKKFDVNGD 60

Query: 61  GKICSSELGSIMRSLGQAATEEELQSMIEEVDADGDGYIDLHEFIELNTKDVDSEEVLAN 120
           GKICSSELGSIMRSLGQ ATEEELQSMIEEVDADGDGYIDLHEFIELN KDVDS+EVLAN
Sbjct: 61  GKICSSELGSIMRSLGQPATEEELQSMIEEVDADGDGYIDLHEFIELNIKDVDSDEVLAN 120

Query: 121 LKDAFSVYDMDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMICFDEFKVM 180
           LKDAFSVYD+DGNGSITAEEL EVLKSLGDECSLADCRKMITGVDKNGDGMI FDEFKVM
Sbjct: 121 LKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVM 180

Query: 181 MMMSGSRSGFN 189
           MMMSGSRSGF+
Sbjct: 181 MMMSGSRSGFS 191

BLAST of Cp4.1LG13g03490 vs. ExPASy TrEMBL
Match: A0A6J1FFM7 (probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC111445291 PE=4 SV=1)

HSP 1 Score: 346 bits (887), Expect = 9.20e-120
Identity = 179/190 (94.21%), Postives = 185/190 (97.37%), Query Frame = 0

Query: 1   MGFKSLFSLKIKKSDASTDSPLRSTAVPLLGSRTPSFNSPAQLAELQQVFKKFDVNGDGK 60
           MGFKSLFSLKIKKSDASTDSPL ST VP+LGSRTPSFNS AQLA+LQQVFKKFDVNGDGK
Sbjct: 1   MGFKSLFSLKIKKSDASTDSPLPSTPVPVLGSRTPSFNSRAQLADLQQVFKKFDVNGDGK 60

Query: 61  ICSSELGSIMRSLGQAATEEELQSMIEEVDADGDGYIDLHEFIELNTKDVDSEEVLANLK 120
           ICSSELGSIMRSLGQ ATEEE+QSMIEEVDADGDGYIDLHEFIELNTKDVDS+EVLANLK
Sbjct: 61  ICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLANLK 120

Query: 121 DAFSVYDMDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMICFDEFKVMMM 180
           DAFSVYD+DGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMI FDEFKVMMM
Sbjct: 121 DAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVMMM 180

Query: 181 MSGSRSGFNA 190
           +SGSRSGFNA
Sbjct: 181 VSGSRSGFNA 190

BLAST of Cp4.1LG13g03490 vs. ExPASy TrEMBL
Match: A0A6J1IDH5 (probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC111472087 PE=4 SV=1)

HSP 1 Score: 340 bits (872), Expect = 1.91e-117
Identity = 179/191 (93.72%), Postives = 184/191 (96.34%), Query Frame = 0

Query: 1   MGFKSLFSLKIKKSDASTDSPLRSTAVPLLGSRTPSFNSPA--QLAELQQVFKKFDVNGD 60
           MGFKSLFSLKIKKSDASTDSPLRSTAVP+LGSRTPSFNS +  QLAELQQVFKKFDVNGD
Sbjct: 1   MGFKSLFSLKIKKSDASTDSPLRSTAVPVLGSRTPSFNSNSREQLAELQQVFKKFDVNGD 60

Query: 61  GKICSSELGSIMRSLGQAATEEELQSMIEEVDADGDGYIDLHEFIELNTKDVDSEEVLAN 120
           GKICSSELGSIMRSLGQ ATEEELQSMIEEVDADGDGYIDLHEFIELN KDVDS+EVLAN
Sbjct: 61  GKICSSELGSIMRSLGQPATEEELQSMIEEVDADGDGYIDLHEFIELNIKDVDSDEVLAN 120

Query: 121 LKDAFSVYDMDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMICFDEFKVM 180
           LKDAFSVYD+DGNGSITAEEL EVLKSLGDECSLADCRKMITGVDKNGDGMI FDEFKVM
Sbjct: 121 LKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVM 180

Query: 181 MMMSGSRSGFN 189
           MMMSGSRSGF+
Sbjct: 181 MMMSGSRSGFS 191

BLAST of Cp4.1LG13g03490 vs. ExPASy TrEMBL
Match: A0A6J1JTS9 (probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC111487441 PE=4 SV=1)

HSP 1 Score: 303 bits (777), Expect = 5.16e-103
Identity = 160/186 (86.02%), Postives = 172/186 (92.47%), Query Frame = 0

Query: 1   MGFKSLFSLKIKKSDASTDSPLRSTAVPLLGSRTPSFNSPAQLAELQQVFKKFDVNGDGK 60
           MGFKSLFS K KKSD+S+DSPL ST VP+LGSRTPSFNS AQ+AEL+QVFKKFDVNGDGK
Sbjct: 1   MGFKSLFSRK-KKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGK 60

Query: 61  ICSSELGSIMRSLGQAATEEELQSMIEEVDADGDGYIDLHEFIELNTKDVDSEEVLANLK 120
           ICSSELGSIM SLGQ ATEEELQ+MI+EVDADGDGYIDLHEFIELNTK VDS+EVL NLK
Sbjct: 61  ICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLK 120

Query: 121 DAFSVYDMDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMICFDEFKVMMM 180
           DAFSVYD+DGNGSITAEEL EVLKSLGD+CSLADCR MI+GVD+NGDGMI FDEFK MMM
Sbjct: 121 DAFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMM 180

Query: 181 MSGSRS 186
            SGSRS
Sbjct: 181 -SGSRS 184

BLAST of Cp4.1LG13g03490 vs. ExPASy TrEMBL
Match: A0A6J1HID9 (probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC111463873 PE=4 SV=1)

HSP 1 Score: 303 bits (777), Expect = 5.16e-103
Identity = 160/186 (86.02%), Postives = 172/186 (92.47%), Query Frame = 0

Query: 1   MGFKSLFSLKIKKSDASTDSPLRSTAVPLLGSRTPSFNSPAQLAELQQVFKKFDVNGDGK 60
           MGFKSLFS K KKSD+S+DSPL ST VP+LGSRTPSFNS AQ+AEL+QVFKKFDVNGDGK
Sbjct: 1   MGFKSLFSRK-KKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGK 60

Query: 61  ICSSELGSIMRSLGQAATEEELQSMIEEVDADGDGYIDLHEFIELNTKDVDSEEVLANLK 120
           ICSSELGSIM SLGQ ATEEELQ+MI+EVDADGDGYIDLHEFIELNTK VDS+EVL NLK
Sbjct: 61  ICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLK 120

Query: 121 DAFSVYDMDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMICFDEFKVMMM 180
           DAFSVYD+DGNGSITAEEL EVLKSLGD+CSLADCR MI+GVD+NGDGMI FDEFK MMM
Sbjct: 121 DAFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMM 180

Query: 181 MSGSRS 186
            SGSRS
Sbjct: 181 -SGSRS 184

BLAST of Cp4.1LG13g03490 vs. ExPASy TrEMBL
Match: A0A6J1CZ20 (probable calcium-binding protein CML25 OS=Momordica charantia OX=3673 GN=LOC111015568 PE=4 SV=1)

HSP 1 Score: 297 bits (760), Expect = 2.01e-100
Identity = 160/186 (86.02%), Postives = 170/186 (91.40%), Query Frame = 0

Query: 1   MGFKSLFSLKIKKSDASTDSPLRSTAVPLLGSRTPSFNSPAQLAELQQVFKKFDVNGDGK 60
           MGFKSLFS K KKSDAS+DSP  STAVP+LGSRTPSFN   Q+AEL+QVFKKFDVNGDGK
Sbjct: 1   MGFKSLFSRK-KKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGK 60

Query: 61  ICSSELGSIMRSLGQAATEEELQSMIEEVDADGDGYIDLHEFIELNTKDVDSEEVLANLK 120
           I SSELGSIM SLGQ ATEEE+Q+MI+EVDADGDG+IDL EFIELNTK VDSEEVL NLK
Sbjct: 61  ISSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLK 120

Query: 121 DAFSVYDMDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMICFDEFKVMMM 180
           DAFSVYD+DGNGSITAEEL EVLKSLGDECSLADCRKMITGVD+NGDGMI FDEFKVMMM
Sbjct: 121 DAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMM 180

Query: 181 MSGSRS 186
            SGSRS
Sbjct: 181 -SGSRS 184

BLAST of Cp4.1LG13g03490 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 200.7 bits (509), Expect = 1.1e-51
Identity = 98/144 (68.06%), Postives = 116/144 (80.56%), Query Frame = 0

Query: 42  QLAELQQVFKKFDVNGDGKICSSELGSIMRSLGQAATEEELQSMIEEVDADGDGYIDLHE 101
           ++ EL+ VFKKFDVNGDGKI S ELG+IM SLG    EEEL+  I E+D  GDGYI+  E
Sbjct: 34  EIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEE 93

Query: 102 FIELNTKDVDSEEVLANLKDAFSVYDMDGNGSITAEELQEVLKSLGDECSLADCRKMITG 161
           F+ELNTK +D  +VL NLKDAFSVYD+DGNGSI+AEEL EVL+SLGDECS+A+CRKMI G
Sbjct: 94  FVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGG 153

Query: 162 VDKNGDGMICFDEFKVMMMMSGSR 186
           VDK+GDG I F+EFK+MM M   R
Sbjct: 154 VDKDGDGTIDFEEFKIMMTMGSRR 177

BLAST of Cp4.1LG13g03490 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 137.9 bits (346), Expect = 8.5e-33
Identity = 67/155 (43.23%), Postives = 107/155 (69.03%), Query Frame = 0

Query: 32  SRTPSFNSPAQLAELQQVFKKFDVNGDGKICSSELGSIMRSLGQAATEEELQSMIEEVDA 91
           S+  S N    + ++++VF++FD N DGKI   EL  ++ +L   A++EE ++M++E D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 92  DGDGYIDLHEFIEL--NTKDVDSEEVLANLKDAFSVYDMDGNGSITAEELQEVLKSLGDE 151
           DG+G+IDL EF+ L   +    +   + +LK+AF +YD+D NG I+A EL  V+K+LG++
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 152 CSLADCRKMITGVDKNGDGMICFDEFKVMMMMSGS 185
           CS+ DC++MI  VD +GDG + F+EFK MMM++GS
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMMMINGS 156

BLAST of Cp4.1LG13g03490 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 136.3 bits (342), Expect = 2.5e-32
Identity = 66/143 (46.15%), Postives = 103/143 (72.03%), Query Frame = 0

Query: 45  ELQQVFKKFDVNGDGKICSSELGSIMRSLGQAATEEELQSMIEEVDADGDGYIDLHEFIE 104
           ++++VF++FD NGDGKI   EL  ++R+L   A+ EE  +M+++ D DG+G+IDL EF+ 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 105 LNTKDV----DSEEVLANLKDAFSVYDMDGNGSITAEELQEVLKSLGDECSLADCRKMIT 164
           L    +    ++   +++LK+AF +YD+DGNG I+A+EL  V+K+LG++CS+ DC+KMI+
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 165 GVDKNGDGMICFDEFKVMMMMSG 184
            VD +GDG + FDEFK MM   G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNGG 159

BLAST of Cp4.1LG13g03490 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 131.3 bits (329), Expect = 8.0e-31
Identity = 73/159 (45.91%), Postives = 98/159 (61.64%), Query Frame = 0

Query: 24  STAVPLLGSRTPSFNSPAQLAELQQVFKKFDVNGDGKICSSELGSIMRSLGQAATEEELQ 83
           ++A P     TP+    A   EL++VF +FD NGDGKI   ELG + +++G + TE EL 
Sbjct: 2   ASANPETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELN 61

Query: 84  SMIEEVDADGDGYIDLHEFIELNTKDVDSEEVLANLKDAFSVYDMDGNGSITAEELQEVL 143
            ++EEVD D DGYI+L EF  L      S    A ++DAF +YD D NG I+A EL +VL
Sbjct: 62  RVLEEVDTDRDGYINLDEFSTL----CRSSSSAAEIRDAFDLYDQDKNGLISASELHQVL 121

Query: 144 KSLGDECSLADCRKMITGVDKNGDGMICFDEFKVMMMMS 183
             LG  CS+ DC +MI  VD +GDG + F+EF+ MM  S
Sbjct: 122 NRLGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSS 156

BLAST of Cp4.1LG13g03490 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 131.3 bits (329), Expect = 8.0e-31
Identity = 73/159 (45.91%), Postives = 98/159 (61.64%), Query Frame = 0

Query: 24  STAVPLLGSRTPSFNSPAQLAELQQVFKKFDVNGDGKICSSELGSIMRSLGQAATEEELQ 83
           ++A P     TP+    A   EL++VF +FD NGDGKI   ELG + +++G + TE EL 
Sbjct: 2   ASANPETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELN 61

Query: 84  SMIEEVDADGDGYIDLHEFIELNTKDVDSEEVLANLKDAFSVYDMDGNGSITAEELQEVL 143
            ++EEVD D DGYI+L EF  L      S    A ++DAF +YD D NG I+A EL +VL
Sbjct: 62  RVLEEVDTDRDGYINLDEFSTL----CRSSSSAAEIRDAFDLYDQDKNGLISASELHQVL 121

Query: 144 KSLGDECSLADCRKMITGVDKNGDGMICFDEFKVMMMMS 183
             LG  CS+ DC +MI  VD +GDG + F+EF+ MM  S
Sbjct: 122 NRLGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSS 156

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FYK21.5e-5068.06Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ... [more]
Q8RZB51.5e-3750.00Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
O649431.3e-3352.59Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2[more]
A0T2M34.9e-3351.11Polcalcin Cup a 4 OS=Hesperocyparis arizonica OX=49011 PE=1 SV=1[more]
Q6L4D41.1e-3241.40Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Match NameE-valueIdentityDescription
XP_023551072.17.82e-128100.00probable calcium-binding protein CML25 [Cucurbita pepo subsp. pepo][more]
KAG7029021.19.42e-12094.74putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. a... [more]
XP_022939351.11.90e-11994.21probable calcium-binding protein CML25 [Cucurbita moschata][more]
KAG6579273.11.56e-11893.68putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. s... [more]
XP_022973553.13.94e-11793.72probable calcium-binding protein CML25 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1FFM79.20e-12094.21probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1IDH51.91e-11793.72probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC1114720... [more]
A0A6J1JTS95.16e-10386.02probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC1114874... [more]
A0A6J1HID95.16e-10386.02probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
A0A6J1CZ202.01e-10086.02probable calcium-binding protein CML25 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
Match NameE-valueIdentityDescription
AT1G24620.11.1e-5168.06EF hand calcium-binding protein family [more]
AT1G66400.18.5e-3343.23calmodulin like 23 [more]
AT5G37770.12.5e-3246.15EF hand calcium-binding protein family [more]
AT1G18210.18.0e-3145.91Calcium-binding EF-hand family protein [more]
AT1G18210.28.0e-3145.91Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 118..146
e-value: 2.6E-5
score: 33.7
coord: 154..182
e-value: 5.3E-5
score: 32.6
coord: 81..109
e-value: 6.2E-5
score: 32.4
coord: 45..73
e-value: 8.6E-7
score: 38.6
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 45..104
e-value: 5.4E-14
score: 52.4
coord: 118..179
e-value: 1.2E-10
score: 41.7
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 41..76
score: 15.099209
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 77..112
score: 12.951263
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 114..149
score: 15.154999
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 150..185
score: 12.616518
IPR002048EF-hand domainCDDcd00051EFhcoord: 45..105
e-value: 1.06109E-19
score: 76.8177
IPR002048EF-hand domainCDDcd00051EFhcoord: 119..180
e-value: 3.37987E-19
score: 75.2769
NoneNo IPR availableGENE3D1.10.238.10coord: 108..185
e-value: 3.1E-24
score: 87.1
coord: 32..107
e-value: 1.4E-25
score: 91.4
NoneNo IPR availablePANTHERPTHR23050:SF382CALCIUM-BINDING PROTEIN CML25-RELATEDcoord: 28..185
NoneNo IPR availablePANTHERPTHR23050CALCIUM BINDING PROTEINcoord: 28..185
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 90..102
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 54..66
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 127..139
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 163..175
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 33..181

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG13g03490.1Cp4.1LG13g03490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050790 regulation of catalytic activity
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0030234 enzyme regulator activity