Cp4.1LG13g03460 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG13g03460
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionE3 ubiquitin protein ligase RIE1-like
LocationCp4.1LG13: 3434254 .. 3436352 (+)
RNA-Seq ExpressionCp4.1LG13g03460
SyntenyCp4.1LG13g03460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAACCTTCGTAGGGAAAAACAAAGGATGAGAATGGGGAGAGAGAGGGGAGAAGAAGAAGGCAGGATTGAAATTTTGGAATCAACAAAGTGAAGAATGGACGTGATACTCCCTTGGATCATGAACTTTCTATCAATTTCTAAACCCACGAACCAGCAGCAGAAGATTCTCCTTCTCAATCAAGACGCCCCCAATTCATAACCCTTCTAATTTCATTCAATCAAATCCTCACCGGCGACCATGCCTTCTCCTTCCATGGAGGCGCCTGCCCAACAGATCCCTCTCTTACCCTCCCGTCATCCCTCCAACTCTGACGCCTCCACTTCCCCCATCGGCCCCCTCGCTGTTCTCTTCGCTCACGCCACTGGCCGCCGTGGAACTTCTATGGTGGTCCGGGAGACAGCGGCGCGTGAACTTGAAGAGCGCCGCATCGATTGGGGGTATTCCAAGCCGGTGGTGGCTCTTGACATTATGTGGAATTTGGCTTTCGTTTTCGCTTCTCTGGGTGTGCTTATTCACTCGGCCGATGAAAAACCTAACACGCCAATTCGGCTTTGGATTGTCGTTTATGCGGCTCAATGCTTGGTGCACGCTGTGCTTGTGTGGTTTGAATTCAGAAGGAGGAATGCGAGGAGGGCTAGGGATATGGAGACGCAGCATAATGCATATGTAGCTAATGAAAGCGATGAAGAAGATGGTACCCCCTGGAATCTCTCCGGGTGAGTAAAACTTCAATTCTCTCTGCTATATGAAGTTGTGACCGTCTTGAACTCAAACATTGCTTATTTTTACTCAAATTTGATATGGGTCTGAACTCGGTTTTGATTGAATTACAAAGCAGTGGATAGCTTTTCTGATTTAAATGTTGTATTGATTGAATGAATGAGTGAGTAAATTGGTCAGGATCGGTTATAGATTCCTGATTTGATGATTTATAAGTCATTAGCATGTAATTGACTTGGTGGTGGATGAGCAGTGTTGCCAAAAGATGTGAGTCAGTGAACACCCTGATATCATTAATTTGGTGGTTGTTTGGCTTTTACTGGGTCGTGGCTGGGGGTAACATCCTAATGCAGAATGCTCCATCTTTATACTGGTATGATTTTGAACCATCGTGATTCTGGCTGTGGTGGGGGGACATGCATCATTTATCTGTTTCTAATTCTGAGTTTATGCAGGTTGGCTGTGGTATTTCTGGCATTTGATGTGTTCTTTGCCATCTTTTGTGTTGTTTTGGCATGTTTGATAGGAATTGCTCTCTGTTGCTGCTTGCCTTGCATTATAGCTATTCTTTATGCTGTTGCAGGCCAGGTAAGAGGAAATCCCTGTTTCATGGCTGCCACACAGAGATGTTAGCCAAATTCAGGCCCCATTGAAACCTGCTAACTTCTTTCTTTCCTTCCTTTCCCCCTCACATCTCAACAGGAAGGTGCAACCGATGCTGATTTAAGTATGCTTCCTAAGTATAGATATCAAGTGTCCAATGAGCCTTCTTCCGGGGATGGTTTGATGGTTCCCGTCGAATCTAGCAGCAGACACTTAACGACTGAGCGTGTTCTATTACGCGAGGATGCGGTAAAGCGAACTCGTTTATCGTTATTCAGAAATGATCTTTCTCCTCTTTGTTGAATCAGTTTTCTTTCTAAATCCTTAATCATCTTTATGATCTTTGTTGTAGGAGTGCTGTATATGCCTTAGTCCTTACGAGAACGGAGCCGAGCTTCATGCTCTTCCATGCAATCACCATTTTCATTATGCGTGTATCACAAAATGGCTGAAGATGAATGCAACATGTCCTCTGTGCAAGTACAACATTCTAAAAAATTGTGAACCCGTGTAACGCTGAAGATGAAACGATGCTGTGAATTCCTGGTATGTACATATAGATCACGTCGTGATCCTGCTTCGTTCTGGTTCAAACTTGTTAGCCTTTTCTTGTTTCCGTTTCTTTGTGTTCATGCTGCCTGATCTCTGCTGTGCTTATTCTGTCTGAGACTACTTTGATCTGTAGCAGAAATTATGAATATCTTTCTGGTCAATGTGTTCAGATTAATATTTCATTATTCTATTTCAATTATTCTCATCCTATTTATTATGGCTT

mRNA sequence

TAACCTTCGTAGGGAAAAACAAAGGATGAGAATGGGGAGAGAGAGGGGAGAAGAAGAAGGCAGGATTGAAATTTTGGAATCAACAAAGTGAAGAATGGACGTGATACTCCCTTGGATCATGAACTTTCTATCAATTTCTAAACCCACGAACCAGCAGCAGAAGATTCTCCTTCTCAATCAAGACGCCCCCAATTCATAACCCTTCTAATTTCATTCAATCAAATCCTCACCGGCGACCATGCCTTCTCCTTCCATGGAGGCGCCTGCCCAACAGATCCCTCTCTTACCCTCCCGTCATCCCTCCAACTCTGACGCCTCCACTTCCCCCATCGGCCCCCTCGCTGTTCTCTTCGCTCACGCCACTGGCCGCCGTGGAACTTCTATGGTGGTCCGGGAGACAGCGGCGCGTGAACTTGAAGAGCGCCGCATCGATTGGGGGTATTCCAAGCCGGTGGTGGCTCTTGACATTATGTGGAATTTGGCTTTCGTTTTCGCTTCTCTGGGTGTGCTTATTCACTCGGCCGATGAAAAACCTAACACGCCAATTCGGCTTTGGATTGTCGTTTATGCGGCTCAATGCTTGGTGCACGCTGTGCTTGTGTGGTTTGAATTCAGAAGGAGGAATGCGAGGAGGGCTAGGGATATGGAGACGCAGCATAATGCATATGTAGCTAATGAAAGCGATGAAGAAGATGGTACCCCCTGGAATCTCTCCGGTGTTGCCAAAAGATGTGAGTCAGTGAACACCCTGATATCATTAATTTGGTGGTTGTTTGGCTTTTACTGGGTCGTGGCTGGGGGTAACATCCTAATGCAGAATGCTCCATCTTTATACTGGTTGGCTGTGGTATTTCTGGCATTTGATGTGTTCTTTGCCATCTTTTGTGTTGTTTTGGCATGTTTGATAGGAATTGCTCTCTGTTGCTGCTTGCCTTGCATTATAGCTATTCTTTATGCTGTTGCAGGCCAGGAAGGTGCAACCGATGCTGATTTAAGTATGCTTCCTAAGTATAGATATCAAGTGTCCAATGAGCCTTCTTCCGGGGATGGTTTGATGGTTCCCGTCGAATCTAGCAGCAGACACTTAACGACTGAGCGTGTTCTATTACGCGAGGATGCGGAGTGCTGTATATGCCTTAGTCCTTACGAGAACGGAGCCGAGCTTCATGCTCTTCCATGCAATCACCATTTTCATTATGCGTGTATCACAAAATGGCTGAAGATGAATGCAACATGTCCTCTGTGCAAGTACAACATTCTAAAAAATTGTGAACCCGTGTAACGCTGAAGATGAAACGATGCTGTGAATTCCTGGTATGTACATATAGATCACGTCGTGATCCTGCTTCGTTCTGGTTCAAACTTGTTAGCCTTTTCTTGTTTCCGTTTCTTTGTGTTCATGCTGCCTGATCTCTGCTGTGCTTATTCTGTCTGAGACTACTTTGATCTGTAGCAGAAATTATGAATATCTTTCTGGTCAATGTGTTCAGATTAATATTTCATTATTCTATTTCAATTATTCTCATCCTATTTATTATGGCTT

Coding sequence (CDS)

ATGCCTTCTCCTTCCATGGAGGCGCCTGCCCAACAGATCCCTCTCTTACCCTCCCGTCATCCCTCCAACTCTGACGCCTCCACTTCCCCCATCGGCCCCCTCGCTGTTCTCTTCGCTCACGCCACTGGCCGCCGTGGAACTTCTATGGTGGTCCGGGAGACAGCGGCGCGTGAACTTGAAGAGCGCCGCATCGATTGGGGGTATTCCAAGCCGGTGGTGGCTCTTGACATTATGTGGAATTTGGCTTTCGTTTTCGCTTCTCTGGGTGTGCTTATTCACTCGGCCGATGAAAAACCTAACACGCCAATTCGGCTTTGGATTGTCGTTTATGCGGCTCAATGCTTGGTGCACGCTGTGCTTGTGTGGTTTGAATTCAGAAGGAGGAATGCGAGGAGGGCTAGGGATATGGAGACGCAGCATAATGCATATGTAGCTAATGAAAGCGATGAAGAAGATGGTACCCCCTGGAATCTCTCCGGTGTTGCCAAAAGATGTGAGTCAGTGAACACCCTGATATCATTAATTTGGTGGTTGTTTGGCTTTTACTGGGTCGTGGCTGGGGGTAACATCCTAATGCAGAATGCTCCATCTTTATACTGGTTGGCTGTGGTATTTCTGGCATTTGATGTGTTCTTTGCCATCTTTTGTGTTGTTTTGGCATGTTTGATAGGAATTGCTCTCTGTTGCTGCTTGCCTTGCATTATAGCTATTCTTTATGCTGTTGCAGGCCAGGAAGGTGCAACCGATGCTGATTTAAGTATGCTTCCTAAGTATAGATATCAAGTGTCCAATGAGCCTTCTTCCGGGGATGGTTTGATGGTTCCCGTCGAATCTAGCAGCAGACACTTAACGACTGAGCGTGTTCTATTACGCGAGGATGCGGAGTGCTGTATATGCCTTAGTCCTTACGAGAACGGAGCCGAGCTTCATGCTCTTCCATGCAATCACCATTTTCATTATGCGTGTATCACAAAATGGCTGAAGATGAATGCAACATGTCCTCTGTGCAAGTACAACATTCTAAAAAATTGTGAACCCGTGTAA

Protein sequence

MPSPSMEAPAQQIPLLPSRHPSNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELEERRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAVLVWFEFRRRNARRARDMETQHNAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWWLFGFYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYAVAGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVPVESSSRHLTTERVLLREDAECCICLSPYENGAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKNCEPV
Homology
BLAST of Cp4.1LG13g03460 vs. ExPASy Swiss-Prot
Match: Q8GUU2 (E3 ubiquitin protein ligase RIE1 OS=Arabidopsis thaliana OX=3702 GN=RIE1 PE=2 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 1.0e-113
Identity = 213/350 (60.86%), Postives = 255/350 (72.86%), Query Frame = 0

Query: 3   SPSMEAPAQQIPLLPSRHPSNSDASTSPIGP--LAVLFAHATGRRGTSMVVRETAARELE 62
           S S  A  Q  PLL  RH  +  +S+S   P  LAVL    TG R  SM+VRETAAR LE
Sbjct: 6   SDSTAARDQHAPLLRPRHDGSFSSSSSSARPTALAVLLGRITGHRAPSMLVRETAARALE 65

Query: 63  ERRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAVL 122
           ERRIDWGYSKPVVA DI+WN A V AS  +L+ + +E+PN PIR+WI VY  QCL H VL
Sbjct: 66  ERRIDWGYSKPVVAADILWNAALVLASAVMLVGTVEERPNEPIRVWICVYGLQCLFHVVL 125

Query: 123 VWFEFRRRNA-RRARDMET----QHNAYVANESDEEDGTPWNLSGVAKRCESVNTLISLI 182
           VW E+ RRN+ RRARD+E+     +N     E D +D +        KRCES+NT+IS I
Sbjct: 126 VWSEYWRRNSTRRARDLESYDHEDYNIEYDYEQDSDDNS--TTYSFVKRCESINTVISFI 185

Query: 183 WWLFGFYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCII 242
           WW+ GFYWVV GG+ L+  AP+LYWL+V+FLA DVFFA+FCVVLACL+GIALCCCLPCII
Sbjct: 186 WWIIGFYWVVEGGDKLLGEAPNLYWLSVIFLAIDVFFAVFCVVLACLVGIALCCCLPCII 245

Query: 243 AILYAVAGQEGATDADLSMLPKYRYQV--SNEPS-SGDGLMVPVESSSRHLTTERVLLRE 302
           A+LYAVAG EG ++A+L +LP Y+++   SNE + +G G MVP+  +   L TER LL E
Sbjct: 246 ALLYAVAGTEGVSEAELGVLPLYKFKAFHSNEKNITGPGKMVPIPINGLCLATERTLLAE 305

Query: 303 DAECCICLSPYENGAELHALPCNHHFHYACITKWLKMNATCPLCKYNILK 343
           DA+CCICLS YE+GAELHALPCNHHFH  CI KWLKM ATCPLCKYNILK
Sbjct: 306 DADCCICLSSYEDGAELHALPCNHHFHSTCIVKWLKMRATCPLCKYNILK 353

BLAST of Cp4.1LG13g03460 vs. ExPASy Swiss-Prot
Match: Q8LDB8 (E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g63170 PE=2 SV=2)

HSP 1 Score: 332.4 bits (851), Expect = 6.1e-90
Identity = 176/343 (51.31%), Postives = 221/343 (64.43%), Query Frame = 0

Query: 35  AVLFAHA-TGR---RGTSMVVRETAARELEERRIDWGYSKPVVALDIMWNLAFVFASLGV 94
           A L  HA +GR   R  SM+VRE AA +LEER+ DW YSKPVV LD +WNLAFV  +  V
Sbjct: 33  ARLLRHASSGRMMMREPSMLVREAAAEQLEERQSDWAYSKPVVVLDFVWNLAFVVVATAV 92

Query: 95  LIHSADEKPNTPIRLWIVVYAAQCLVHAVLVWFEFRRRNARRARDMETQHNAYVANES-- 154
           L+ S+DE PN P+R+WI+ Y  QC++H V V  E+RRRN+RR RD+  + ++  ++ S  
Sbjct: 93  LVLSSDENPNMPLRVWIIGYGLQCMMHMVCVCVEYRRRNSRRRRDLSPRSSSSSSSSSSS 152

Query: 155 -DEEDGTPWNL----------------SGVAKRCESVNTLISLIWWLFGFYWVVAGGNIL 214
            DEE+G   +                 +  AK  ES NT+IS IWW+ GFYWV +GG  L
Sbjct: 153 MDEEEGLGLSRNSDERYLELGQLENENNSFAKHLESANTMISFIWWVIGFYWVSSGGQEL 212

Query: 215 MQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYAVAGQEGATDAD 274
            Q +P LYWL +VFL FDVFF +FCV LAC+IGIA+CCCLPCIIA+LYAVA QEGA+  D
Sbjct: 213 AQGSPQLYWLCIVFLGFDVFFVVFCVALACVIGIAVCCCLPCIIAVLYAVAEQEGASKED 272

Query: 275 LSMLPKYRYQ---------VSNEPSSGD--GLMVPVESSSRHLTTERVLLREDAECCICL 334
           +  L K++++         V  E   GD  G+M    + S     E  L  EDAECCICL
Sbjct: 273 IDQLTKFKFRKVGDTMKHTVDEEQGQGDSGGVMTECGTDS---PVEHALPHEDAECCICL 332

Query: 335 SPYENGAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKN 344
           S YE+  EL  LPC HHFH  C+ KWL +NATCPLCKYNILK+
Sbjct: 333 SAYEDETELRELPCGHHFHCGCVDKWLYINATCPLCKYNILKS 372

BLAST of Cp4.1LG13g03460 vs. ExPASy Swiss-Prot
Match: Q93Z92 (E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g11680 PE=2 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 2.0e-88
Identity = 173/338 (51.18%), Postives = 211/338 (62.43%), Query Frame = 0

Query: 20  HPSNSDASTSPIGPLAVLFAHATGR---RGTSMVVRETAARELEERRIDWGYSKPVVALD 79
           +P +  A    +   A    HA  R   R  SM+VRETAA +LEER+ DW YSKPVV LD
Sbjct: 54  NPIHRSARRQGLREAARFLRHAGSRRMMREPSMLVRETAAEQLEERQSDWAYSKPVVFLD 113

Query: 80  IMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAVLVWFEFRRRNARRARD- 139
           I+WNLAFV   + VLI S DEKPN P+R+W+V Y  QC +H   V  E+RRR  RR  + 
Sbjct: 114 ILWNLAFVAIGVAVLILSRDEKPNMPLRVWVVGYGIQCWLHMACVCVEYRRRRRRRHPED 173

Query: 140 ------METQHNAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWWLFGFYWVVAGGN 199
                   +    YV+    E+ G     S  AK  ES NT+ S IWW+ GFYWV AGG 
Sbjct: 174 GGGSGLTNSSSQQYVSLAQLEDRG---ETSNPAKHLESANTMFSFIWWIIGFYWVSAGGQ 233

Query: 200 ILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYAVAGQEGATD 259
            L  ++P LYWL ++FL FDVFF +FCV LAC+IG+A+CCCLPCIIAILYAVA QEGA+ 
Sbjct: 234 TLSSDSPQLYWLCIIFLGFDVFFVVFCVALACVIGLAVCCCLPCIIAILYAVADQEGASK 293

Query: 260 ADLSMLPKYRY----QVSNEPSSGDGLMVPVESSSRHLTTERVLLREDAECCICLSPYEN 319
            D+  +PK+R+     V        G+M    + S     ER L  EDAECCICL  YE+
Sbjct: 294 NDIDQMPKFRFTKTGNVEKLSGKARGIMTECGTDS---PIERSLSPEDAECCICLCEYED 353

Query: 320 GAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKN 344
           G EL  LPCNHHFH  CI KWL +N+ CPLCK+NILKN
Sbjct: 354 GVELRELPCNHHFHCTCIDKWLHINSRCPLCKFNILKN 385

BLAST of Cp4.1LG13g03460 vs. ExPASy Swiss-Prot
Match: Q9LN71 (E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g12760 PE=1 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 1.1e-86
Identity = 166/331 (50.15%), Postives = 215/331 (64.95%), Query Frame = 0

Query: 45  RGTSMVVRETAARELEERRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIR 104
           R  SM+VRE AA +LEER+ DW YSKPVV LDI+WNLAFV  +  +L+ S  E P  P+R
Sbjct: 73  REPSMLVREAAAEQLEERQSDWAYSKPVVVLDIVWNLAFVSVATAILVMSRKEHPIMPLR 132

Query: 105 LWIVVYAAQCLVHAVLVWFEFRRRNARRA---------RDMETQHNAYVANESDEEDGTP 164
           +W++ YA QC++H V V  E+RRRN RR          R   +  ++  ++  +E  G+ 
Sbjct: 133 VWLLGYALQCVLHMVCVCVEYRRRNRRRTNRTTTTTPPRSRSSSSSSSSSSLEEEALGSR 192

Query: 165 WN--------------LSGVAKRCESVNTLISLIWWLFGFYWVVAGGNILMQNAPSLYWL 224
            N               S VAK  ES NT+ S IWW+ GFYWV AGG  L Q +P +YWL
Sbjct: 193 RNSGVQDLSLGHLDTESSSVAKHLESANTMFSFIWWIIGFYWVSAGGQELAQESPRIYWL 252

Query: 225 AVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYAVAGQEGATDADLSMLPKYRYQ 284
           ++VFL FDVFF +FCV LAC+IGIA+CCCLPCIIA+LYAVA QEGA+  D+  L K++++
Sbjct: 253 SIVFLGFDVFFVVFCVALACVIGIAVCCCLPCIIAVLYAVADQEGASKEDIEQLTKFKFR 312

Query: 285 V-------SNEPSSG--DGLMVPVESSSRHLTTERVLLREDAECCICLSPYENGAELHAL 344
                   +N+ + G  +G+M    + S     E  LL+EDAECCICLS YE+G EL  L
Sbjct: 313 KLGDANKHTNDEAQGTTEGIMTECGTDS---PIEHTLLQEDAECCICLSAYEDGTELREL 372

BLAST of Cp4.1LG13g03460 vs. ExPASy Swiss-Prot
Match: Q08CG8 (RING finger protein 44 OS=Danio rerio OX=7955 GN=rnf44 PE=2 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 8.7e-12
Identity = 34/96 (35.42%), Postives = 43/96 (44.79%), Query Frame = 0

Query: 242 AGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVPVESSSRHLTTERVLLREDAECCICLS 301
           A   G T AD+  LP YR+ + N  S                        E   C +C S
Sbjct: 366 AKPRGLTKADIEQLPSYRFNLENHQS------------------------EQTLCVVCFS 425

Query: 302 PYENGAELHALPCNHHFHYACITKWLKMNATCPLCK 338
            +E+   L  LPCNH FH  C+ KWLK N TCP+C+
Sbjct: 426 DFESRQLLRVLPCNHEFHAKCVDKWLKTNRTCPICR 437

BLAST of Cp4.1LG13g03460 vs. NCBI nr
Match: XP_023551599.1 (E3 ubiquitin protein ligase RIE1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 703 bits (1815), Expect = 1.17e-255
Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 0

Query: 1   MPSPSMEAPAQQIPLLPSRHPSNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELE 60
           MPSPSMEAPAQQIPLLPSRHPSNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELE
Sbjct: 1   MPSPSMEAPAQQIPLLPSRHPSNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELE 60

Query: 61  ERRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAVL 120
           ERRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAVL
Sbjct: 61  ERRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAVL 120

Query: 121 VWFEFRRRNARRARDMETQHNAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWWLFG 180
           VWFEFRRRNARRARDMETQHNAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWWLFG
Sbjct: 121 VWFEFRRRNARRARDMETQHNAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWWLFG 180

Query: 181 FYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYA 240
           FYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYA
Sbjct: 181 FYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYA 240

Query: 241 VAGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVPVESSSRHLTTERVLLREDAECCICL 300
           VAGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVPVESSSRHLTTERVLLREDAECCICL
Sbjct: 241 VAGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVPVESSSRHLTTERVLLREDAECCICL 300

Query: 301 SPYENGAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKNCEPV 347
           SPYENGAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKNCEPV
Sbjct: 301 SPYENGAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKNCEPV 347

BLAST of Cp4.1LG13g03460 vs. NCBI nr
Match: XP_022938968.1 (E3 ubiquitin protein ligase RIE1-like [Cucurbita moschata])

HSP 1 Score: 692 bits (1786), Expect = 3.10e-251
Identity = 342/347 (98.56%), Postives = 344/347 (99.14%), Query Frame = 0

Query: 1   MPSPSMEAPAQQIPLLPSRHPSNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELE 60
           M SPSMEAPAQQIPLLPSRHPSNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELE
Sbjct: 1   MSSPSMEAPAQQIPLLPSRHPSNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELE 60

Query: 61  ERRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAVL 120
           ERRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYA QCLVHAVL
Sbjct: 61  ERRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYAVQCLVHAVL 120

Query: 121 VWFEFRRRNARRARDMETQHNAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWWLFG 180
           VWFEFRRRNARRARDMETQHNAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWWLFG
Sbjct: 121 VWFEFRRRNARRARDMETQHNAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWWLFG 180

Query: 181 FYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYA 240
           FYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYA
Sbjct: 181 FYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYA 240

Query: 241 VAGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVPVESSSRHLTTERVLLREDAECCICL 300
           VAGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVP+ESSSRHLTTERVLLREDAECCICL
Sbjct: 241 VAGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVPIESSSRHLTTERVLLREDAECCICL 300

Query: 301 SPYENGAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKNCEPV 347
           SPYE+GAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKN EPV
Sbjct: 301 SPYEDGAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKNYEPV 347

BLAST of Cp4.1LG13g03460 vs. NCBI nr
Match: XP_022973549.1 (E3 ubiquitin protein ligase RIE1-like [Cucurbita maxima])

HSP 1 Score: 688 bits (1775), Expect = 1.47e-249
Identity = 341/347 (98.27%), Postives = 343/347 (98.85%), Query Frame = 0

Query: 1   MPSPSMEAPAQQIPLLPSRHPSNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELE 60
           M SPSMEAPAQQIPLLPSR PSNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELE
Sbjct: 1   MSSPSMEAPAQQIPLLPSRPPSNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELE 60

Query: 61  ERRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAVL 120
           ERRIDWGYSKPVVALDIMWNLAFVF SLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAVL
Sbjct: 61  ERRIDWGYSKPVVALDIMWNLAFVFVSLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAVL 120

Query: 121 VWFEFRRRNARRARDMETQHNAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWWLFG 180
           VWFEFRRRNARRARDME QHNAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWWLFG
Sbjct: 121 VWFEFRRRNARRARDMEMQHNAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWWLFG 180

Query: 181 FYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYA 240
           FYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYA
Sbjct: 181 FYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYA 240

Query: 241 VAGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVPVESSSRHLTTERVLLREDAECCICL 300
           VAGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVPVESSSR+LTTERVLLREDAECCICL
Sbjct: 241 VAGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVPVESSSRNLTTERVLLREDAECCICL 300

Query: 301 SPYENGAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKNCEPV 347
           SPYE+GAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKNCEPV
Sbjct: 301 SPYEDGAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKNCEPV 347

BLAST of Cp4.1LG13g03460 vs. NCBI nr
Match: KAG6579275.1 (E3 ubiquitin protein ligase RIE1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 671 bits (1730), Expect = 1.94e-242
Identity = 332/337 (98.52%), Postives = 334/337 (99.11%), Query Frame = 0

Query: 1   MPSPSMEAPAQQIPLLPSRHPSNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELE 60
           M SPSMEAPAQQIPLLPSRHPSNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELE
Sbjct: 1   MSSPSMEAPAQQIPLLPSRHPSNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELE 60

Query: 61  ERRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAVL 120
           ERRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYA QCLVHAVL
Sbjct: 61  ERRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYAVQCLVHAVL 120

Query: 121 VWFEFRRRNARRARDMETQHNAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWWLFG 180
           VWFEFRRRNAR ARDMETQHNAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWWLFG
Sbjct: 121 VWFEFRRRNARGARDMETQHNAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWWLFG 180

Query: 181 FYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYA 240
           FYWVVAGGNIL+QNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYA
Sbjct: 181 FYWVVAGGNILIQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYA 240

Query: 241 VAGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVPVESSSRHLTTERVLLREDAECCICL 300
           VAGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVPVESSSRHLTTERVLLREDAECCICL
Sbjct: 241 VAGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVPVESSSRHLTTERVLLREDAECCICL 300

Query: 301 SPYENGAELHALPCNHHFHYACITKWLKMNATCPLCK 337
           SPYE+GAELHALPCNHHFHYACITKWLKMNATCPLCK
Sbjct: 301 SPYEDGAELHALPCNHHFHYACITKWLKMNATCPLCK 337

BLAST of Cp4.1LG13g03460 vs. NCBI nr
Match: XP_023521351.1 (E3 ubiquitin protein ligase RIE1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 634 bits (1636), Expect = 2.82e-228
Identity = 315/353 (89.24%), Postives = 333/353 (94.33%), Query Frame = 0

Query: 1   MPSPSMEAPAQQIPLLPSRHPSNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELE 60
           M SPSMEAPAQQIPLLPSRHPSNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELE
Sbjct: 1   MSSPSMEAPAQQIPLLPSRHPSNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELE 60

Query: 61  ERRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAVL 120
           ERRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAVL
Sbjct: 61  ERRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAVL 120

Query: 121 VWFEFRRRNARRARDMET-QH-----NAYVANESDEEDGTPWNLSGVAKRCESVNTLISL 180
           VWFEFRRRNARRA DMET QH     + Y +NES++EDG+P +LSGVA+RCE+VNTL+SL
Sbjct: 121 VWFEFRRRNARRAMDMETPQHPQPDIDGYFSNESNDEDGSPRSLSGVARRCETVNTLVSL 180

Query: 181 IWWLFGFYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCI 240
           +WWL GFYWV+AGGN+L+QNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCI
Sbjct: 181 LWWLLGFYWVMAGGNLLLQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCI 240

Query: 241 IAILYAVAGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVPVESSSRHLTTERVLLREDA 300
           IAILYAVAGQEGAT+ADLS L KYRYQVSN PS GDGLMVP+E+SSR+LTTE VLLREDA
Sbjct: 241 IAILYAVAGQEGATEADLSTLSKYRYQVSNNPSPGDGLMVPIETSSRYLTTEHVLLREDA 300

Query: 301 ECCICLSPYENGAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKNCEPV 347
           ECCICLS YE+G ELHALPCNHHFHYAC TKWLKMNATCPLCKYNILKNCEPV
Sbjct: 301 ECCICLSSYEDGVELHALPCNHHFHYACSTKWLKMNATCPLCKYNILKNCEPV 353

BLAST of Cp4.1LG13g03460 vs. ExPASy TrEMBL
Match: A0A6J1FKD5 (E3 ubiquitin protein ligase RIE1-like OS=Cucurbita moschata OX=3662 GN=LOC111445019 PE=4 SV=1)

HSP 1 Score: 692 bits (1786), Expect = 1.50e-251
Identity = 342/347 (98.56%), Postives = 344/347 (99.14%), Query Frame = 0

Query: 1   MPSPSMEAPAQQIPLLPSRHPSNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELE 60
           M SPSMEAPAQQIPLLPSRHPSNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELE
Sbjct: 1   MSSPSMEAPAQQIPLLPSRHPSNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELE 60

Query: 61  ERRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAVL 120
           ERRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYA QCLVHAVL
Sbjct: 61  ERRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYAVQCLVHAVL 120

Query: 121 VWFEFRRRNARRARDMETQHNAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWWLFG 180
           VWFEFRRRNARRARDMETQHNAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWWLFG
Sbjct: 121 VWFEFRRRNARRARDMETQHNAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWWLFG 180

Query: 181 FYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYA 240
           FYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYA
Sbjct: 181 FYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYA 240

Query: 241 VAGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVPVESSSRHLTTERVLLREDAECCICL 300
           VAGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVP+ESSSRHLTTERVLLREDAECCICL
Sbjct: 241 VAGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVPIESSSRHLTTERVLLREDAECCICL 300

Query: 301 SPYENGAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKNCEPV 347
           SPYE+GAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKN EPV
Sbjct: 301 SPYEDGAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKNYEPV 347

BLAST of Cp4.1LG13g03460 vs. ExPASy TrEMBL
Match: A0A6J1IEW9 (E3 ubiquitin protein ligase RIE1-like OS=Cucurbita maxima OX=3661 GN=LOC111472083 PE=4 SV=1)

HSP 1 Score: 688 bits (1775), Expect = 7.13e-250
Identity = 341/347 (98.27%), Postives = 343/347 (98.85%), Query Frame = 0

Query: 1   MPSPSMEAPAQQIPLLPSRHPSNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELE 60
           M SPSMEAPAQQIPLLPSR PSNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELE
Sbjct: 1   MSSPSMEAPAQQIPLLPSRPPSNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELE 60

Query: 61  ERRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAVL 120
           ERRIDWGYSKPVVALDIMWNLAFVF SLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAVL
Sbjct: 61  ERRIDWGYSKPVVALDIMWNLAFVFVSLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAVL 120

Query: 121 VWFEFRRRNARRARDMETQHNAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWWLFG 180
           VWFEFRRRNARRARDME QHNAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWWLFG
Sbjct: 121 VWFEFRRRNARRARDMEMQHNAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWWLFG 180

Query: 181 FYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYA 240
           FYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYA
Sbjct: 181 FYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYA 240

Query: 241 VAGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVPVESSSRHLTTERVLLREDAECCICL 300
           VAGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVPVESSSR+LTTERVLLREDAECCICL
Sbjct: 241 VAGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVPVESSSRNLTTERVLLREDAECCICL 300

Query: 301 SPYENGAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKNCEPV 347
           SPYE+GAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKNCEPV
Sbjct: 301 SPYEDGAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKNCEPV 347

BLAST of Cp4.1LG13g03460 vs. ExPASy TrEMBL
Match: A0A0A0KRG7 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G561780 PE=4 SV=1)

HSP 1 Score: 618 bits (1594), Expect = 3.83e-222
Identity = 309/350 (88.29%), Postives = 323/350 (92.29%), Query Frame = 0

Query: 3   SPSMEAPAQQIPLLPSRHP-SNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELEE 62
           SPSM+ PAQQIPLLPSR P SNSDASTSPIGPLA+L   ATGRRGTSM+VRETAARELEE
Sbjct: 7   SPSMDPPAQQIPLLPSRQPPSNSDASTSPIGPLALLLGRATGRRGTSMLVRETAARELEE 66

Query: 63  RRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAVLV 122
           RRIDWGYSKPVVALDI WNLAFVF SLGVLIHS  EKPNTPIRLWIVVYAAQCLVH VLV
Sbjct: 67  RRIDWGYSKPVVALDITWNLAFVFVSLGVLIHSVHEKPNTPIRLWIVVYAAQCLVHVVLV 126

Query: 123 WFEFRRRNARRARDMETQH----NAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWW 182
           WFEF+RRNARRARDMETQ     + YV NESDE+DG   + S VAKRCESVNT++SLIWW
Sbjct: 127 WFEFKRRNARRARDMETQQQQEPDGYVTNESDEDDGGQRSFSSVAKRCESVNTMVSLIWW 186

Query: 183 LFGFYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAI 242
           LFGFYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFA+FCVVLACLIGIALCCCLPCIIAI
Sbjct: 187 LFGFYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAVFCVVLACLIGIALCCCLPCIIAI 246

Query: 243 LYAVAGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVPVESSSRHLTTERVLLREDAECC 302
           LYAVAGQEGATDADLSMLPKYRY+VSNEPS GDGLMVPVE+SSR+LTTERVLL EDAECC
Sbjct: 247 LYAVAGQEGATDADLSMLPKYRYRVSNEPSPGDGLMVPVETSSRYLTTERVLLCEDAECC 306

Query: 303 ICLSPYENGAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKNCEPV 347
           ICLSPYE+G ELHALPCNHHFHYACITKWLKMNATCPLCKYNILKNCE V
Sbjct: 307 ICLSPYEDGVELHALPCNHHFHYACITKWLKMNATCPLCKYNILKNCEHV 356

BLAST of Cp4.1LG13g03460 vs. ExPASy TrEMBL
Match: A0A5A7TTE4 (E3 ubiquitin protein ligase RIE1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold663G00270 PE=4 SV=1)

HSP 1 Score: 617 bits (1590), Expect = 1.56e-221
Identity = 309/348 (88.79%), Postives = 322/348 (92.53%), Query Frame = 0

Query: 5   SMEAPAQQIPLLPSRHP-SNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELEERR 64
           SM+ PAQQIPLLPSR P SNSDASTSPIGPLA+L   ATGRRGTSM+VRETAARELEERR
Sbjct: 9   SMDPPAQQIPLLPSRQPPSNSDASTSPIGPLALLLGRATGRRGTSMLVRETAARELEERR 68

Query: 65  IDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAVLVWF 124
           IDWGYSKPVVALDI WNLAFVF SLGVLI S  EKPNTPIRLWIVVYAAQCLVH VLVWF
Sbjct: 69  IDWGYSKPVVALDITWNLAFVFVSLGVLIRSVHEKPNTPIRLWIVVYAAQCLVHVVLVWF 128

Query: 125 EFRRRNARRARDMETQH----NAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWWLF 184
           EF+RRNARRARDMETQ     + YV NESDE+DG   +LS VAKRCESVNTL+SLIWWLF
Sbjct: 129 EFKRRNARRARDMETQQQQQPDGYVTNESDEDDGGQRSLSSVAKRCESVNTLVSLIWWLF 188

Query: 185 GFYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILY 244
           GFYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFA+FCVVLACLIGIALCCCLPCIIAILY
Sbjct: 189 GFYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAVFCVVLACLIGIALCCCLPCIIAILY 248

Query: 245 AVAGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVPVESSSRHLTTERVLLREDAECCIC 304
           AVAGQEGATDADLSMLPKYRY+VSNEPS GDGLMVPVE+SSR+LTTERVLLREDAECCIC
Sbjct: 249 AVAGQEGATDADLSMLPKYRYRVSNEPSPGDGLMVPVETSSRYLTTERVLLREDAECCIC 308

Query: 305 LSPYENGAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKNCEPV 347
           LSPYE+G ELHALPCNHHFHYACITKWLKMNATCPLCKYNILKN EPV
Sbjct: 309 LSPYEDGVELHALPCNHHFHYACITKWLKMNATCPLCKYNILKNSEPV 356

BLAST of Cp4.1LG13g03460 vs. ExPASy TrEMBL
Match: E5GB66 (Zinc finger protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 617 bits (1590), Expect = 1.56e-221
Identity = 309/348 (88.79%), Postives = 322/348 (92.53%), Query Frame = 0

Query: 5   SMEAPAQQIPLLPSRHP-SNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAARELEERR 64
           SM+ PAQQIPLLPSR P SNSDASTSPIGPLA+L   ATGRRGTSM+VRETAARELEERR
Sbjct: 9   SMDPPAQQIPLLPSRQPPSNSDASTSPIGPLALLLGRATGRRGTSMLVRETAARELEERR 68

Query: 65  IDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAVLVWF 124
           IDWGYSKPVVALDI WNLAFVF SLGVLI S  EKPNTPIRLWIVVYAAQCLVH VLVWF
Sbjct: 69  IDWGYSKPVVALDITWNLAFVFVSLGVLIRSVHEKPNTPIRLWIVVYAAQCLVHVVLVWF 128

Query: 125 EFRRRNARRARDMETQH----NAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWWLF 184
           EF+RRNARRARDMETQ     + YV NESDE+DG   +LS VAKRCESVNTL+SLIWWLF
Sbjct: 129 EFKRRNARRARDMETQQQQQPDGYVTNESDEDDGGQRSLSSVAKRCESVNTLVSLIWWLF 188

Query: 185 GFYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILY 244
           GFYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFA+FCVVLACLIGIALCCCLPCIIAILY
Sbjct: 189 GFYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAVFCVVLACLIGIALCCCLPCIIAILY 248

Query: 245 AVAGQEGATDADLSMLPKYRYQVSNEPSSGDGLMVPVESSSRHLTTERVLLREDAECCIC 304
           AVAGQEGATDADLSMLPKYRY+VSNEPS GDGLMVPVE+SSR+LTTERVLLREDAECCIC
Sbjct: 249 AVAGQEGATDADLSMLPKYRYRVSNEPSPGDGLMVPVETSSRYLTTERVLLREDAECCIC 308

Query: 305 LSPYENGAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKNCEPV 347
           LSPYE+G ELHALPCNHHFHYACITKWLKMNATCPLCKYNILKN EPV
Sbjct: 309 LSPYEDGVELHALPCNHHFHYACITKWLKMNATCPLCKYNILKNSEPV 356

BLAST of Cp4.1LG13g03460 vs. TAIR 10
Match: AT1G68070.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 422.5 bits (1085), Expect = 3.2e-118
Identity = 224/351 (63.82%), Postives = 264/351 (75.21%), Query Frame = 0

Query: 1   MPSP-SMEAPAQQIPLLPSRHPSNSDASTSPIGPLAVLFAHATGRRGTSMVVRETAAREL 60
           M SP S        PLL SR    S     P+  +AVL   A+GRRG SMVVRETAA+EL
Sbjct: 1   MSSPESPSGSDSSTPLLRSR---QSSPRRQPV--IAVLLNRASGRRGASMVVRETAAQEL 60

Query: 61  EERRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAV 120
           EERR DWGYSKPVVALD++WN AFV  ++ +L+   +EKPN PIR+WI  YA QCLVH V
Sbjct: 61  EERRADWGYSKPVVALDMLWNTAFVLVAIVMLLVFKEEKPNVPIRIWICGYAIQCLVHVV 120

Query: 121 LVWFEFRRRNAR-RARDMETQHNAYVAN-ESDEEDGTPWNLSGVAKRCESVNTLISLIWW 180
           LVW EFR+RNAR R  D+E    A   N +S++ED     LS   K CES+NT+IS +WW
Sbjct: 121 LVWLEFRKRNARSRPGDLEA---AQATNQDSEDEDNDERFLS--TKTCESMNTIISFVWW 180

Query: 181 LFGFYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAI 240
           + GFYW+V+GG+IL+QNA  LYWL  VFLAFDVFFAIFCVVLACLIGIALCCCLPCIIA+
Sbjct: 181 IVGFYWLVSGGDILLQNATHLYWLTFVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAL 240

Query: 241 LYAVAGQEGATDADLSMLPKYRYQVSN---EPSSGDGLMVPVESSSRHLTTERVLLREDA 300
           LYAVAGQEGA++ADLS+LPKYR+   N   + S G G M+PV+++S +L  ERVLL EDA
Sbjct: 241 LYAVAGQEGASEADLSILPKYRFHTMNNDEKQSDGGGKMIPVDAASENLGNERVLLPEDA 300

Query: 301 ECCICLSPYENGAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKNCE 346
           +CCICLS YE+GAEL +LPCNHHFH  CI KWLKMNATCPLCK+NILK  E
Sbjct: 301 DCCICLSSYEDGAELVSLPCNHHFHSTCIVKWLKMNATCPLCKFNILKGNE 341

BLAST of Cp4.1LG13g03460 vs. TAIR 10
Match: AT2G01735.1 (RING-finger protein for embryogenesis )

HSP 1 Score: 411.4 bits (1056), Expect = 7.3e-115
Identity = 213/350 (60.86%), Postives = 255/350 (72.86%), Query Frame = 0

Query: 3   SPSMEAPAQQIPLLPSRHPSNSDASTSPIGP--LAVLFAHATGRRGTSMVVRETAARELE 62
           S S  A  Q  PLL  RH  +  +S+S   P  LAVL    TG R  SM+VRETAAR LE
Sbjct: 6   SDSTAARDQHAPLLRPRHDGSFSSSSSSARPTALAVLLGRITGHRAPSMLVRETAARALE 65

Query: 63  ERRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAVL 122
           ERRIDWGYSKPVVA DI+WN A V AS  +L+ + +E+PN PIR+WI VY  QCL H VL
Sbjct: 66  ERRIDWGYSKPVVAADILWNAALVLASAVMLVGTVEERPNEPIRVWICVYGLQCLFHVVL 125

Query: 123 VWFEFRRRNA-RRARDMET----QHNAYVANESDEEDGTPWNLSGVAKRCESVNTLISLI 182
           VW E+ RRN+ RRARD+E+     +N     E D +D +        KRCES+NT+IS I
Sbjct: 126 VWSEYWRRNSTRRARDLESYDHEDYNIEYDYEQDSDDNS--TTYSFVKRCESINTVISFI 185

Query: 183 WWLFGFYWVVAGGNILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCII 242
           WW+ GFYWVV GG+ L+  AP+LYWL+V+FLA DVFFA+FCVVLACL+GIALCCCLPCII
Sbjct: 186 WWIIGFYWVVEGGDKLLGEAPNLYWLSVIFLAIDVFFAVFCVVLACLVGIALCCCLPCII 245

Query: 243 AILYAVAGQEGATDADLSMLPKYRYQV--SNEPS-SGDGLMVPVESSSRHLTTERVLLRE 302
           A+LYAVAG EG ++A+L +LP Y+++   SNE + +G G MVP+  +   L TER LL E
Sbjct: 246 ALLYAVAGTEGVSEAELGVLPLYKFKAFHSNEKNITGPGKMVPIPINGLCLATERTLLAE 305

Query: 303 DAECCICLSPYENGAELHALPCNHHFHYACITKWLKMNATCPLCKYNILK 343
           DA+CCICLS YE+GAELHALPCNHHFH  CI KWLKM ATCPLCKYNILK
Sbjct: 306 DADCCICLSSYEDGAELHALPCNHHFHSTCIVKWLKMRATCPLCKYNILK 353

BLAST of Cp4.1LG13g03460 vs. TAIR 10
Match: AT1G63170.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 332.4 bits (851), Expect = 4.3e-91
Identity = 176/343 (51.31%), Postives = 221/343 (64.43%), Query Frame = 0

Query: 35  AVLFAHA-TGR---RGTSMVVRETAARELEERRIDWGYSKPVVALDIMWNLAFVFASLGV 94
           A L  HA +GR   R  SM+VRE AA +LEER+ DW YSKPVV LD +WNLAFV  +  V
Sbjct: 33  ARLLRHASSGRMMMREPSMLVREAAAEQLEERQSDWAYSKPVVVLDFVWNLAFVVVATAV 92

Query: 95  LIHSADEKPNTPIRLWIVVYAAQCLVHAVLVWFEFRRRNARRARDMETQHNAYVANES-- 154
           L+ S+DE PN P+R+WI+ Y  QC++H V V  E+RRRN+RR RD+  + ++  ++ S  
Sbjct: 93  LVLSSDENPNMPLRVWIIGYGLQCMMHMVCVCVEYRRRNSRRRRDLSPRSSSSSSSSSSS 152

Query: 155 -DEEDGTPWNL----------------SGVAKRCESVNTLISLIWWLFGFYWVVAGGNIL 214
            DEE+G   +                 +  AK  ES NT+IS IWW+ GFYWV +GG  L
Sbjct: 153 MDEEEGLGLSRNSDERYLELGQLENENNSFAKHLESANTMISFIWWVIGFYWVSSGGQEL 212

Query: 215 MQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYAVAGQEGATDAD 274
            Q +P LYWL +VFL FDVFF +FCV LAC+IGIA+CCCLPCIIA+LYAVA QEGA+  D
Sbjct: 213 AQGSPQLYWLCIVFLGFDVFFVVFCVALACVIGIAVCCCLPCIIAVLYAVAEQEGASKED 272

Query: 275 LSMLPKYRYQ---------VSNEPSSGD--GLMVPVESSSRHLTTERVLLREDAECCICL 334
           +  L K++++         V  E   GD  G+M    + S     E  L  EDAECCICL
Sbjct: 273 IDQLTKFKFRKVGDTMKHTVDEEQGQGDSGGVMTECGTDS---PVEHALPHEDAECCICL 332

Query: 335 SPYENGAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKN 344
           S YE+  EL  LPC HHFH  C+ KWL +NATCPLCKYNILK+
Sbjct: 333 SAYEDETELRELPCGHHFHCGCVDKWLYINATCPLCKYNILKS 372

BLAST of Cp4.1LG13g03460 vs. TAIR 10
Match: AT4G11680.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 327.4 bits (838), Expect = 1.4e-89
Identity = 173/338 (51.18%), Postives = 211/338 (62.43%), Query Frame = 0

Query: 20  HPSNSDASTSPIGPLAVLFAHATGR---RGTSMVVRETAARELEERRIDWGYSKPVVALD 79
           +P +  A    +   A    HA  R   R  SM+VRETAA +LEER+ DW YSKPVV LD
Sbjct: 54  NPIHRSARRQGLREAARFLRHAGSRRMMREPSMLVRETAAEQLEERQSDWAYSKPVVFLD 113

Query: 80  IMWNLAFVFASLGVLIHSADEKPNTPIRLWIVVYAAQCLVHAVLVWFEFRRRNARRARD- 139
           I+WNLAFV   + VLI S DEKPN P+R+W+V Y  QC +H   V  E+RRR  RR  + 
Sbjct: 114 ILWNLAFVAIGVAVLILSRDEKPNMPLRVWVVGYGIQCWLHMACVCVEYRRRRRRRHPED 173

Query: 140 ------METQHNAYVANESDEEDGTPWNLSGVAKRCESVNTLISLIWWLFGFYWVVAGGN 199
                   +    YV+    E+ G     S  AK  ES NT+ S IWW+ GFYWV AGG 
Sbjct: 174 GGGSGLTNSSSQQYVSLAQLEDRG---ETSNPAKHLESANTMFSFIWWIIGFYWVSAGGQ 233

Query: 200 ILMQNAPSLYWLAVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYAVAGQEGATD 259
            L  ++P LYWL ++FL FDVFF +FCV LAC+IG+A+CCCLPCIIAILYAVA QEGA+ 
Sbjct: 234 TLSSDSPQLYWLCIIFLGFDVFFVVFCVALACVIGLAVCCCLPCIIAILYAVADQEGASK 293

Query: 260 ADLSMLPKYRY----QVSNEPSSGDGLMVPVESSSRHLTTERVLLREDAECCICLSPYEN 319
            D+  +PK+R+     V        G+M    + S     ER L  EDAECCICL  YE+
Sbjct: 294 NDIDQMPKFRFTKTGNVEKLSGKARGIMTECGTDS---PIERSLSPEDAECCICLCEYED 353

Query: 320 GAELHALPCNHHFHYACITKWLKMNATCPLCKYNILKN 344
           G EL  LPCNHHFH  CI KWL +N+ CPLCK+NILKN
Sbjct: 354 GVELRELPCNHHFHCTCIDKWLHINSRCPLCKFNILKN 385

BLAST of Cp4.1LG13g03460 vs. TAIR 10
Match: AT1G12760.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 321.6 bits (823), Expect = 7.6e-88
Identity = 166/331 (50.15%), Postives = 215/331 (64.95%), Query Frame = 0

Query: 45  RGTSMVVRETAARELEERRIDWGYSKPVVALDIMWNLAFVFASLGVLIHSADEKPNTPIR 104
           R  SM+VRE AA +LEER+ DW YSKPVV LDI+WNLAFV  +  +L+ S  E P  P+R
Sbjct: 73  REPSMLVREAAAEQLEERQSDWAYSKPVVVLDIVWNLAFVSVATAILVMSRKEHPIMPLR 132

Query: 105 LWIVVYAAQCLVHAVLVWFEFRRRNARRA---------RDMETQHNAYVANESDEEDGTP 164
           +W++ YA QC++H V V  E+RRRN RR          R   +  ++  ++  +E  G+ 
Sbjct: 133 VWLLGYALQCVLHMVCVCVEYRRRNRRRTNRTTTTTPPRSRSSSSSSSSSSLEEEALGSR 192

Query: 165 WN--------------LSGVAKRCESVNTLISLIWWLFGFYWVVAGGNILMQNAPSLYWL 224
            N               S VAK  ES NT+ S IWW+ GFYWV AGG  L Q +P +YWL
Sbjct: 193 RNSGVQDLSLGHLDTESSSVAKHLESANTMFSFIWWIIGFYWVSAGGQELAQESPRIYWL 252

Query: 225 AVVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAILYAVAGQEGATDADLSMLPKYRYQ 284
           ++VFL FDVFF +FCV LAC+IGIA+CCCLPCIIA+LYAVA QEGA+  D+  L K++++
Sbjct: 253 SIVFLGFDVFFVVFCVALACVIGIAVCCCLPCIIAVLYAVADQEGASKEDIEQLTKFKFR 312

Query: 285 V-------SNEPSSG--DGLMVPVESSSRHLTTERVLLREDAECCICLSPYENGAELHAL 344
                   +N+ + G  +G+M    + S     E  LL+EDAECCICLS YE+G EL  L
Sbjct: 313 KLGDANKHTNDEAQGTTEGIMTECGTDS---PIEHTLLQEDAECCICLSAYEDGTELREL 372

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GUU21.0e-11360.86E3 ubiquitin protein ligase RIE1 OS=Arabidopsis thaliana OX=3702 GN=RIE1 PE=2 SV... [more]
Q8LDB86.1e-9051.31E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g631... [more]
Q93Z922.0e-8851.18E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g116... [more]
Q9LN711.1e-8650.15E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g127... [more]
Q08CG88.7e-1235.42RING finger protein 44 OS=Danio rerio OX=7955 GN=rnf44 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023551599.11.17e-255100.00E3 ubiquitin protein ligase RIE1-like [Cucurbita pepo subsp. pepo][more]
XP_022938968.13.10e-25198.56E3 ubiquitin protein ligase RIE1-like [Cucurbita moschata][more]
XP_022973549.11.47e-24998.27E3 ubiquitin protein ligase RIE1-like [Cucurbita maxima][more]
KAG6579275.11.94e-24298.52E3 ubiquitin protein ligase RIE1, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_023521351.12.82e-22889.24E3 ubiquitin protein ligase RIE1-like isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1FKD51.50e-25198.56E3 ubiquitin protein ligase RIE1-like OS=Cucurbita moschata OX=3662 GN=LOC111445... [more]
A0A6J1IEW97.13e-25098.27E3 ubiquitin protein ligase RIE1-like OS=Cucurbita maxima OX=3661 GN=LOC11147208... [more]
A0A0A0KRG73.83e-22288.29RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G561780 P... [more]
A0A5A7TTE41.56e-22188.79E3 ubiquitin protein ligase RIE1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
E5GB661.56e-22188.79Zinc finger protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G68070.13.2e-11863.82Zinc finger, C3HC4 type (RING finger) family protein [more]
AT2G01735.17.3e-11560.86RING-finger protein for embryogenesis [more]
AT1G63170.14.3e-9151.31Zinc finger, C3HC4 type (RING finger) family protein [more]
AT4G11680.11.4e-8951.18Zinc finger, C3HC4 type (RING finger) family protein [more]
AT1G12760.17.6e-8850.15Zinc finger, C3HC4 type (RING finger) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 296..336
e-value: 2.5E-7
score: 40.3
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 295..337
e-value: 5.0E-14
score: 52.4
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 296..337
score: 12.902362
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 276..343
e-value: 1.4E-18
score: 67.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..27
NoneNo IPR availablePANTHERPTHR45977TARGET OF ERK KINASE MPK-1coord: 11..343
NoneNo IPR availablePANTHERPTHR45977:SF11E3 UBIQUITIN PROTEIN LIGASE RIE1-LIKEcoord: 11..343
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 283..342

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG13g03460.1Cp4.1LG13g03460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane