Cp4.1LG13g02550 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG13g02550
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionalpha/beta-Hydrolases superfamily protein
LocationCp4.1LG13: 2018719 .. 2023341 (+)
RNA-Seq ExpressionCp4.1LG13g02550
SyntenyCp4.1LG13g02550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACACACACGTAGCCACTAACCAATAATTCACAATTTTCCCCCAACAAATTCTCTCCCTCTCAAGAATCCAATCCACCATGTTCTTCTCCAGATCCAATTTTCCCCCAAAATGCACACAATCTCCCAAATTCCTAAGCCCAAAATCAGACGTTGACTTCCAGGTACAATCTGTTCTTGGCTAACTTTAGATTTATATGCATTTCGGATTTGAGTTTCTACGAATGGTTCTCGAGGGAGTTTATAGCTAAGTCCACAGCATTCACTCAGTTTCCTCTGTCTCTCTTGTTAATTTGAAGAAATCCAGCGTTTAATTCCACATTTTTCCATATTTGAAACTCTGATTCTTAGTTAAGAATGTTCTTGTATGATTCTGATTCTCAAATTATTGACCGGAAGTGTGAATTTGTCCCTATCTTCTTCGTTTTCACTTCCGTTGCTTCTGTTTTTTGCTCTTGATGCTCGAAATTTGAAACCTAGATCTTCCGTTGTTTTCGTGTTCATCCCTAAATTAGTTTAGCTCCATGGGATTCAGTGATTGGAAGCTACTGGAAGGTTCATATGGTATTGGTTGATGAATGAATTCCCGCATTCAAGTATTTGTGTTCTTCTCTTGTTATTTGATTCTTTGGAAAGAAGTTAAAGATTTATTTTCTTGTGAAGTATGCTTTCTAGTTCGCCCAAAATGATCGATCGAAATATTTTGAAGTTCTTCGGCGCCCCATAATCATCTACCACCTGTTATCAACTCCAAGACTTGTATCAATAGAGCTTGTAGTTTCCTTGCATCAAATTTCTTTATGTTTCTTCATAACTCGGGCATTTACTAGAAAAATCTGTATGATCTATCATTCCTTTGAGCTTAGATCATAGGCAACAATGTCTATCATATGTGGTGTACCTATCCTTGAGTGCGTATGCTGTCTGGGATGTGCACGTTGGGTGTGGAAACGCTGTCTCCACACAGCTGGTCATGACAGTGAAACTTGGGGCTTTGCCACGGCCGATGAGTTTGAACCTATTCCCCGAATTTGTCGATATATCCTAGCTGTGTATGAAGATGATATTCGAAAACCTCTTTGGGAACCAGTTGGTGGGTATGGAATCAATCCAGATTGGTTAATCTTGAAGAAGACATACAAAGATACACGAGGGCGGGCTCCTCCATATATTTTATATCTCGATCATGATCATGCCGATATTGTTCTTGCCATCAGGGGATTGAATTTGGCAAAGGAGAGTGATTATGCAGTTTTATTGGACAACAAGCTGGGGAAGATGAAATTTGATGGTGGATATGTTCACAATGGGCTTCTGAAGGCAGCTGGATGGGTTTTGGACAGTGAGAATGAAACTTTGAAGGATTTGGTGAAGAAATATCCGGATTATACCTTGACGTTTGCTGGGCATTCTCTTGGCTCTGGAGTAGCAGCCATGCTAACTTTGGTAGTAGTACAGAATCACGAAAAATTGGAAAATATTGATCGGAAGCGGATAAGGTGCTATGCTATCGCTCCTGCTAGGTGTATGTCCCTGAATTTGGCTGTTAGATATGCAGATGTCATCAACTCTGTTGTTCTTCAGGTAAACTACAGTTTTTTTCTTTAGCTATCATTTTCATTATAAATCGATATTGTATTCTGTAAAAGAGAACAATCTTTTTTGTTGTACGTGTACAAAGTCCCGACTGTTCAAAAATATTTTTTGATTATCGTTTATTGAAGCTGAATATGTGTCAAATTTATACTTGCATCTCAAAATGTAACGGCGATTATTATACTGGACCAGGATGACTTTTTACCCAGGACAGCCACACCCTTGGAAGACATTTTTAAGTCACTCTTCTGGTATGGTTCAGGATTCATGACCCTTTATGGTGTTAACTCCTTTTACGTTGTTGACAACTCGATTGCTGCAAGCATGGAAACTAGAGCTCTATTCTTGCAATGTGAACTCATTCTTATATTAACGCTCATTGCATAAAAGTGAAAACAAAATCTATGCGTTCTTATGAAAAGAATAAATTGGAAATGGTAGTTTAGATTTATTCAGTGTGATTCTTAGTTTCTCAATCATCATTTGAAATTCTCTTCCCAACCATGAATATTCATAAATTGTTGTGTTTCCCTTTTAGTTTGCCATGCCTTCTATGCCTGGGGTGCCTGCGGGATACATGCATATCGGAGGACAAGATGCTTAAAGATCCGAGAAGACTTTACACACCCGGTCGACTCTATCACATTGTTGAGCGAAAGCCCTTCAGGTAAATGCCATGTATTTTCCATATTAAGTTGGTTTATGTAATGGAGAATTCATGTTTCGTTCAAACAAGAAAATGTACCACTTAATACCGGGCTTGTTGTGTGGAAAGGAATTAACGTTTTATTGCGAGCATCTGTTCAAAAAGTTGAGATTCTGAGTTTATGGCCTTCCCTTACTTGTTGTGTGGAAAGAAAGCCTCTCTTAACCTTATCAATTCTTAAAATGTTGGCTTTCTGACCATTGAACCGGAAACATGTCCTGCTCTAACATTTGAATGAATTCATCTGTTTCTTGCGAAAGGTGTGGAAGGTTTCCGCCAGTTGTGAAGACGGCCGTCCCGGTGGATGGGAGGTTTGAACACATAGTTCTTTCTTGTAATGCAACTTCTGACCATGCTATCATTTGGATTGAGAAAGAAGCCAAATGGGCCCTGGAAGTAAGTTTCATCATTCAACCAAACTTTTATGCTGATTGCTTGAAGTTTAATGAAATTTTGGAATATGAGCATTCTTTTGCATTTCATTATATTACATTTTCATCTGCGGTTCACGTTTCCCTCTCTATAATTGTCCACGAAGTCGAAGTTGCCATTGGTGAATCAAATGTTAAGTTGTCACCCCACATCCCTCTCTATAATTGTCCACGAAGTCAAAGTTGCCATTGATGAATCAAATTTTAAGTTGTCACCCCACATCCCCGAATCGCACGCTCGAAAACTGCTCGAGTAATTTGCTATGGTGTGCTGCGAATTGAATTCCGATTCTTTAAAGTTTGGTGGACGCTGTTTATCTTGATCGCTTGAACTACTTTTAGATGTTGTATATCAGTAGACATAGATGGGTGCTTTTGCCGATTTCTTCTCGAAAACCAAGACAATTATTGTTCTCTTTGGGCTTTACCTTTTAGGCTTTTCCTCAAGGTTTTTGAAACGTGTCTTTAGAGGTTTTGAAACGTGGTTCTCACAATTCACCTCCTTTCGCTACCCAGTGTCCTCGCTGACACTCGTTCCTCTCTCCAATCAACATGGGATCTCGTAATCCACCCCCTTCAGGCCTAATGTCCTCGTTGGCACACCGCCCGGTGTCTGGCTCTGGTACCATTTATAATGGCCCAAACCCACCGCGAGCAGATATTGTCCTCTTTGGGCTTTCCCTCAAGGTTTTTAAAAAGTGTCTGCTAGGGAGAGGGTTCCACATCTTATAAAGAATGCTTCGTTCCCTTCTCCAACCAATGTGGGGTCTTACAATCCATCCTCCTTCGAGGCTAGCGTCCTCGCTAACCTTCGTTTCTCTCAAATCAATATGGATCTCACAATTATTAGACATGTTATTCCTTCAGTTTATCCTTCAGTTTACTGAAAAAGAAACTATCTGATATCTGATCTACACTGTTTATTTTAGATTCAGGGCACAAAGATCAATGGTTTCTTTCTGCACTGCGAAAGTTTAAGATCCATGATCATTCAAAACAAGGAGTTCCACCATCTTATGATTAACATGAAGATTATCATCATTCTCCCAAATTTAAAATGTCAAATTTTAGCTGCCTTATCTTGTTCATTGAATGTATTCATGTGAATATAATGCGTGTATTGTCGTAATTTACGTACAAGTACAATGAAATCATTGCTGAAAAACGAAAAACATCCATCTGCTTCTGTCAACTTCAGTTAATGCTGGAGGATAATAAGGTCATGGAGATACCACCCCAACAAAAGATGGAGAGGCAAAATACTTTAGCAAGAGAGCACACTGAAGAGTACAAGGCTGCATTGCAGCGGGCCGTGACGTTATCTGTGCCACATGCATACGCACTTTCCCCGTATGGAACCTTCAGCGAAACAGTTGAAGGTGAAGAAGAAAAAGAAGAAGAATCACCAGCATCGAGTGGAGGGTCATCGAGGAAGAGGAAAGAAACTTGGGATGAACTGATCGAGCGTCTTTACGACAAGGATGACTCAAGACACAACAAGAATGAGTCGAGATACACCGTGCTGAAGAAATCATTCAGTAGTATTTGACCCTACGGATTATATTCATTCATATCAATACTTGGTTACCTGATGAAGCTTCGAGGATGTCGATTTTCTTGGATTCATCCGAAGTTTGAGGCGTGATGACGTTCAGCAATTGTTTAAATTCAGGAAGTGAAAATTGGTAGTGTGTGGAGGTAAGCTAGTCCATATTTTTTGTTAGGATTAGCTCATTCTATACAATTGTTTAAATGTTTGAGTATATTATATACCAATAGCTCTATGTAATAGTTGGTGACCAAGCTTCACATTCTCATCCACTAATACAAGATCCATGCTTCCCTTTGATATTTGCTTTTGGACAAAATATACTTCTGGTTGCTGAAGTTAAG

mRNA sequence

ACACACACGTAGCCACTAACCAATAATTCACAATTTTCCCCCAACAAATTCTCTCCCTCTCAAGAATCCAATCCACCATGTTCTTCTCCAGATCCAATTTTCCCCCAAAATGCACACAATCTCCCAAATTCCTAAGCCCAAAATCAGACGTTGACTTCCAGGTACAATCTGTTCTTGGCTAACTTTAGATTTATATGCATTTCGGATTTGAGTTTCTACGAATGGTTCTCGAGGGAGTTTATAGCTAAGTCCACAGCATTCACTCAGTTTCCTCTGTCTCTCTTGTTAATTTGAAGAAATCCAGCGTTTAATTCCACATTTTTCCATATTTGAAACTCTGATTCTTAGTTAAGAATGTTCTTGTATGATTCTGATTCTCAAATTATTGACCGGAAGTGTGAATTTGTCCCTATCTTCTTCGTTTTCACTTCCGTTGCTTCTGTTTTTTGCTCTTGATGCTCGAAATTTGAAACCTAGATCTTCCGTTGTTTTCGTGTTCATCCCTAAATTAGTTTAGCTCCATGGGATTCAGTGATTGGAAGCTACTGGAAGGTTCATATGGTATTGGTTGATGAATGAATTCCCGCATTCAAGTATTTGTGTTCTTCTCTTGTTATTTGATTCTTTGGAAAGAAGTTAAAGATTTATTTTCTTGTGAAGTATGCTTTCTAGTTCGCCCAAAATGATCGATCGAAATATTTTGAAGTTCTTCGGCGCCCCATAATCATCTACCACCTGTTATCAACTCCAAGACTTGTATCAATAGAGCTTGTAGTTTCCTTGCATCAAATTTCTTTATGTTTCTTCATAACTCGGGCATTTACTAGAAAAATCTGTATGATCTATCATTCCTTTGAGCTTAGATCATAGGCAACAATGTCTATCATATGTGGTGTACCTATCCTTGAGTGCGTATGCTGTCTGGGATGTGCACGTTGGGTGTGGAAACGCTGTCTCCACACAGCTGGTCATGACAGTGAAACTTGGGGCTTTGCCACGGCCGATGAGTTTGAACCTATTCCCCGAATTTGTCGATATATCCTAGCTGTGTATGAAGATGATATTCGAAAACCTCTTTGGGAACCAGTTGGTGGGTATGGAATCAATCCAGATTGGTTAATCTTGAAGAAGACATACAAAGATACACGAGGGCGGGCTCCTCCATATATTTTATATCTCGATCATGATCATGCCGATATTGTTCTTGCCATCAGGGGATTGAATTTGGCAAAGGAGAGTGATTATGCAGTTTTATTGGACAACAAGCTGGGGAAGATGAAATTTGATGGTGGATATGTTCACAATGGGCTTCTGAAGGCAGCTGGATGGGTTTTGGACAGTGAGAATGAAACTTTGAAGGATTTGGTGAAGAAATATCCGGATTATACCTTGACGTTTGCTGGGCATTCTCTTGGCTCTGGAGTAGCAGCCATGCTAACTTTGGTAGTAGTACAGAATCACGAAAAATTGGAAAATATTGATCGGAAGCGGATAAGGTGCTATGCTATCGCTCCTGCTAGGTGTATGTCCCTGAATTTGGCTGTTAGATATGCAGATGTCATCAACTCTGTTGTTCTTCAGGATGACTTTTTACCCAGGACAGCCACACCCTTGGAAGACATTTTTAAGTCACTCTTCTGTTTGCCATGCCTTCTATGCCTGGGGTGCCTGCGGGATACATGCATATCGGAGGACAAGATGCTTAAAGATCCGAGAAGACTTTACACACCCGGTCGACTCTATCACATTGTTGAGCGAAAGCCCTTCAGGTGTGGAAGGTTTCCGCCAGTTGTGAAGACGGCCGTCCCGGTGGATGGGAGGTTTGAACACATAGTTCTTTCTTGTAATGCAACTTCTGACCATGCTATCATTTGGATTGAGAAAGAAGCCAAATGGGCCCTGGAATTAATGCTGGAGGATAATAAGGTCATGGAGATACCACCCCAACAAAAGATGGAGAGGCAAAATACTTTAGCAAGAGAGCACACTGAAGAGTACAAGGCTGCATTGCAGCGGGCCGTGACGTTATCTGTGCCACATGCATACGCACTTTCCCCGTATGGAACCTTCAGCGAAACAGTTGAAGGTGAAGAAGAAAAAGAAGAAGAATCACCAGCATCGAGTGGAGGGTCATCGAGGAAGAGGAAAGAAACTTGGGATGAACTGATCGAGCGTCTTTACGACAAGGATGACTCAAGACACAACAAGAATGAGTCGAGATACACCGTGCTGAAGAAATCATTCAGTAGTATTTGACCCTACGGATTATATTCATTCATATCAATACTTGGTTACCTGATGAAGCTTCGAGGATGTCGATTTTCTTGGATTCATCCGAAGTTTGAGGCGTGATGACGTTCAGCAATTGTTTAAATTCAGGAAGTGAAAATTGGTAGTGTGTGGAGGTAAGCTAGTCCATATTTTTTGTTAGGATTAGCTCATTCTATACAATTGTTTAAATGTTTGAGTATATTATATACCAATAGCTCTATGTAATAGTTGGTGACCAAGCTTCACATTCTCATCCACTAATACAAGATCCATGCTTCCCTTTGATATTTGCTTTTGGACAAAATATACTTCTGGTTGCTGAAGTTAAG

Coding sequence (CDS)

ATGTCTATCATATGTGGTGTACCTATCCTTGAGTGCGTATGCTGTCTGGGATGTGCACGTTGGGTGTGGAAACGCTGTCTCCACACAGCTGGTCATGACAGTGAAACTTGGGGCTTTGCCACGGCCGATGAGTTTGAACCTATTCCCCGAATTTGTCGATATATCCTAGCTGTGTATGAAGATGATATTCGAAAACCTCTTTGGGAACCAGTTGGTGGGTATGGAATCAATCCAGATTGGTTAATCTTGAAGAAGACATACAAAGATACACGAGGGCGGGCTCCTCCATATATTTTATATCTCGATCATGATCATGCCGATATTGTTCTTGCCATCAGGGGATTGAATTTGGCAAAGGAGAGTGATTATGCAGTTTTATTGGACAACAAGCTGGGGAAGATGAAATTTGATGGTGGATATGTTCACAATGGGCTTCTGAAGGCAGCTGGATGGGTTTTGGACAGTGAGAATGAAACTTTGAAGGATTTGGTGAAGAAATATCCGGATTATACCTTGACGTTTGCTGGGCATTCTCTTGGCTCTGGAGTAGCAGCCATGCTAACTTTGGTAGTAGTACAGAATCACGAAAAATTGGAAAATATTGATCGGAAGCGGATAAGGTGCTATGCTATCGCTCCTGCTAGGTGTATGTCCCTGAATTTGGCTGTTAGATATGCAGATGTCATCAACTCTGTTGTTCTTCAGGATGACTTTTTACCCAGGACAGCCACACCCTTGGAAGACATTTTTAAGTCACTCTTCTGTTTGCCATGCCTTCTATGCCTGGGGTGCCTGCGGGATACATGCATATCGGAGGACAAGATGCTTAAAGATCCGAGAAGACTTTACACACCCGGTCGACTCTATCACATTGTTGAGCGAAAGCCCTTCAGGTGTGGAAGGTTTCCGCCAGTTGTGAAGACGGCCGTCCCGGTGGATGGGAGGTTTGAACACATAGTTCTTTCTTGTAATGCAACTTCTGACCATGCTATCATTTGGATTGAGAAAGAAGCCAAATGGGCCCTGGAATTAATGCTGGAGGATAATAAGGTCATGGAGATACCACCCCAACAAAAGATGGAGAGGCAAAATACTTTAGCAAGAGAGCACACTGAAGAGTACAAGGCTGCATTGCAGCGGGCCGTGACGTTATCTGTGCCACATGCATACGCACTTTCCCCGTATGGAACCTTCAGCGAAACAGTTGAAGGTGAAGAAGAAAAAGAAGAAGAATCACCAGCATCGAGTGGAGGGTCATCGAGGAAGAGGAAAGAAACTTGGGATGAACTGATCGAGCGTCTTTACGACAAGGATGACTCAAGACACAACAAGAATGAGTCGAGATACACCGTGCTGAAGAAATCATTCAGTAGTATTTGA

Protein sequence

MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYEDDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKESDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLGSGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLPRTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCGRFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKMERQNTLAREHTEEYKAALQRAVTLSVPHAYALSPYGTFSETVEGEEEKEEESPASSGGSSRKRKETWDELIERLYDKDDSRHNKNESRYTVLKKSFSSI
Homology
BLAST of Cp4.1LG13g02550 vs. ExPASy Swiss-Prot
Match: Q8NCG7 (Diacylglycerol lipase-beta OS=Homo sapiens OX=9606 GN=DAGLB PE=1 SV=2)

HSP 1 Score: 60.5 bits (145), Expect = 5.9e-08
Identity = 45/160 (28.12%), Postives = 76/160 (47.50%), Query Frame = 0

Query: 96  PYILYLDHDHADIVLAIRGLNLAKE--SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVL 155
           P+++ LDH    +V+A+RG    ++  +D +   +    + +      H G+ +AA +V 
Sbjct: 357 PFLVALDHRKESVVVAVRGTMSLQDVLTDLSAESEVLDVECEVQDRLAHKGISQAARYVY 416

Query: 156 DS--ENETLKDLVKKYPDYTLTFAGHSLGSGVAAMLTLVVVQNHEKLENIDRKRIRCYAI 215
                +  L       P+Y L   GHSLG G AA+L  ++   +         ++RCYA 
Sbjct: 417 QRLINDGILSQAFSIAPEYRLVIVGHSLGGGAAALLATMLRAAY--------PQVRCYAF 476

Query: 216 APARCMSLNLAVRYA-DVINSVVLQDDFLPR-TATPLEDI 250
           +P R +       Y+   I S+VL  D +PR + T LED+
Sbjct: 477 SPPRGLWSKALQEYSQSFIVSLVLGKDVIPRLSVTNLEDL 508

BLAST of Cp4.1LG13g02550 vs. ExPASy Swiss-Prot
Match: P0C1S9 (Diacylglycerol lipase-beta OS=Rattus norvegicus OX=10116 GN=Daglb PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 5.9e-08
Identity = 43/160 (26.88%), Postives = 79/160 (49.38%), Query Frame = 0

Query: 96  PYILYLDHDHADIVLAIRGLNLAKE--SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVL 155
           P+I+ LDH    +V+A+RG    ++  +D +   +N    ++      H G+ +AA ++ 
Sbjct: 357 PFIVVLDHRKESVVVAVRGTMSLQDVLTDLSAESENLELDIELQDCVAHKGIAQAARYIY 416

Query: 156 DS--ENETLKDLVKKYPDYTLTFAGHSLGSGVAAMLTLVVVQNHEKLENIDRKRIRCYAI 215
                +  L       P+Y L   GHSLG+G AA+L +++   +         ++R YA 
Sbjct: 417 RRLVNDGILSQAFSVAPEYRLVVVGHSLGAGAAALLAIMLRGAY--------PQVRAYAF 476

Query: 216 APAR-CMSLNLAVRYADVINSVVLQDDFLPR-TATPLEDI 250
           +P R  +S +L     D + S++L  D +PR +   +ED+
Sbjct: 477 SPPRGLLSKSLFEYSKDFVVSLILGMDVIPRLSVANMEDL 508

BLAST of Cp4.1LG13g02550 vs. ExPASy Swiss-Prot
Match: Q91WC9 (Diacylglycerol lipase-beta OS=Mus musculus OX=10090 GN=Daglb PE=1 SV=2)

HSP 1 Score: 59.3 bits (142), Expect = 1.3e-07
Identity = 43/160 (26.88%), Postives = 79/160 (49.38%), Query Frame = 0

Query: 96  PYILYLDHDHADIVLAIRGLNLAKE--SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVL 155
           P+I+ LDH    +V+A+RG    ++  +D +   +     ++      H G+ +AA ++ 
Sbjct: 357 PFIVVLDHRKESVVVAVRGTMSLQDVLTDLSAESETLELGIELQDCVAHKGIAQAARYIH 416

Query: 156 DS--ENETLKDLVKKYPDYTLTFAGHSLGSGVAAMLTLVVVQNHEKLENIDRKRIRCYAI 215
                +  L       P+Y L   GHSLG+G AA+L +++   +         ++R YA 
Sbjct: 417 RRLVNDGILSQAFSVAPEYQLVLVGHSLGAGAAALLAIMLRGAY--------PQVRAYAF 476

Query: 216 APAR-CMSLNLAVRYADVINSVVLQDDFLPR-TATPLEDI 250
           +P R  +S +L     D + S++L  D +PR + T +ED+
Sbjct: 477 SPPRGLLSKSLYEYSKDFVVSLILGMDVIPRLSVTNMEDL 508

BLAST of Cp4.1LG13g02550 vs. ExPASy Swiss-Prot
Match: Q6WQJ1 (Diacylglycerol lipase-alpha OS=Mus musculus OX=10090 GN=Dagla PE=1 SV=2)

HSP 1 Score: 53.9 bits (128), Expect = 5.5e-06
Identity = 55/242 (22.73%), Postives = 103/242 (42.56%), Query Frame = 0

Query: 96  PYILYLDHDHADIVLAIRGLNLAKESDYAVLLD-NKLGKMKFDGGYV-HNGLLKAAGWVL 155
           P+ + +DHD   +V++IRG    K++   +  D  +L      G ++ H G++ +A ++ 
Sbjct: 381 PFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHRGTWLGHKGMVLSAEYIK 440

Query: 156 DSENETL-------KDLVKKYPDYTLTFAGHSLGSGVAAMLTLVVVQNHEKLENIDRKRI 215
               + +       +DL +    Y L   GHSLG+G AA+L+ ++   +  L        
Sbjct: 441 KKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTL-------- 500

Query: 216 RCYAIAPARCMSLNLAVRYA-DVINSVVLQDDFLPRTA--------TPLEDIFKSLFCLP 275
           +C+A +P   +    A+ Y+ + + +VVL  D +PR            L D+ +      
Sbjct: 501 KCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPK 560

Query: 276 CLLCLG---CLRDTCISEDKM--------------------LKDPRRLYTPGRLYHIVER 297
             + +G   C+  + + ED++                    L     LY PGR+ H+V  
Sbjct: 561 WRIIVGATKCIPKSELPEDQVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHN 614

BLAST of Cp4.1LG13g02550 vs. ExPASy Swiss-Prot
Match: Q5YLM1 (Diacylglycerol lipase-alpha OS=Rattus norvegicus OX=10116 GN=Dagla PE=1 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 5.5e-06
Identity = 55/242 (22.73%), Postives = 103/242 (42.56%), Query Frame = 0

Query: 96  PYILYLDHDHADIVLAIRGLNLAKESDYAVLLD-NKLGKMKFDGGYV-HNGLLKAAGWVL 155
           P+ + +DHD   +V++IRG    K++   +  D  +L      G ++ H G++ +A ++ 
Sbjct: 381 PFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHRGTWLGHKGMVLSAEYIK 440

Query: 156 DSENETL-------KDLVKKYPDYTLTFAGHSLGSGVAAMLTLVVVQNHEKLENIDRKRI 215
               + +       +DL +    Y L   GHSLG+G AA+L+ ++   +  L        
Sbjct: 441 KKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTL-------- 500

Query: 216 RCYAIAPARCMSLNLAVRYA-DVINSVVLQDDFLPRTA--------TPLEDIFKSLFCLP 275
           +C+A +P   +    A+ Y+ + + +VVL  D +PR            L D+ +      
Sbjct: 501 KCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPK 560

Query: 276 CLLCLG---CLRDTCISEDKM--------------------LKDPRRLYTPGRLYHIVER 297
             + +G   C+  + + ED++                    L     LY PGR+ H+V  
Sbjct: 561 WRIIVGATKCIPKSELPEDQVEVTALASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHN 614

BLAST of Cp4.1LG13g02550 vs. NCBI nr
Match: XP_023550858.1 (uncharacterized protein LOC111808873 [Cucurbita pepo subsp. pepo] >XP_023550859.1 uncharacterized protein LOC111808873 [Cucurbita pepo subsp. pepo] >XP_023550860.1 uncharacterized protein LOC111808873 [Cucurbita pepo subsp. pepo] >XP_023550861.1 uncharacterized protein LOC111808873 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 938 bits (2425), Expect = 0.0
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE 60
           MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE
Sbjct: 1   MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKE 120
           DDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKE
Sbjct: 61  DDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKE 120

Query: 121 SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG
Sbjct: 121 SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG 180

Query: 181 SGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240
           SGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKM 360

Query: 361 ERQNTLAREHTEEYKAALQRAVTLSVPHAYALSPYGTFSETVEGEEEKEEESPASSGGSS 420
           ERQNTLAREHTEEYKAALQRAVTLSVPHAYALSPYGTFSETVEGEEEKEEESPASSGGSS
Sbjct: 361 ERQNTLAREHTEEYKAALQRAVTLSVPHAYALSPYGTFSETVEGEEEKEEESPASSGGSS 420

Query: 421 RKRKETWDELIERLYDKDDSRHNKNESRYTVLKKSFSSI 459
           RKRKETWDELIERLYDKDDSRHNKNESRYTVLKKSFSSI
Sbjct: 421 RKRKETWDELIERLYDKDDSRHNKNESRYTVLKKSFSSI 459

BLAST of Cp4.1LG13g02550 vs. NCBI nr
Match: XP_022922227.1 (uncharacterized protein LOC111430275 [Cucurbita moschata] >XP_022922228.1 uncharacterized protein LOC111430275 [Cucurbita moschata] >XP_022922229.1 uncharacterized protein LOC111430275 [Cucurbita moschata] >XP_022922230.1 uncharacterized protein LOC111430275 [Cucurbita moschata])

HSP 1 Score: 934 bits (2413), Expect = 0.0
Identity = 456/459 (99.35%), Postives = 458/459 (99.78%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE 60
           MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE
Sbjct: 1   MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKE 120
           DDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKE
Sbjct: 61  DDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKE 120

Query: 121 SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG
Sbjct: 121 SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG 180

Query: 181 SGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240
           SGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKM 360

Query: 361 ERQNTLAREHTEEYKAALQRAVTLSVPHAYALSPYGTFSETVEGEEEKEEESPASSGGSS 420
           ERQNTLAREHTEEYKAALQRAVTL+VPHAY LSPYGTFSETVEGEEEKEEESPASSGGSS
Sbjct: 361 ERQNTLAREHTEEYKAALQRAVTLAVPHAYTLSPYGTFSETVEGEEEKEEESPASSGGSS 420

Query: 421 RKRKETWDELIERLYDKDDSRHNKNESRYTVLKKSFSSI 459
           RKRKETWDELIERLYDKDDSRH+KNESRYTVLKKSFSSI
Sbjct: 421 RKRKETWDELIERLYDKDDSRHDKNESRYTVLKKSFSSI 459

BLAST of Cp4.1LG13g02550 vs. NCBI nr
Match: KAG6579384.1 (hypothetical protein SDJN03_23832, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016864.1 hypothetical protein SDJN02_21975 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 931 bits (2405), Expect = 0.0
Identity = 455/459 (99.13%), Postives = 457/459 (99.56%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE 60
           MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE
Sbjct: 1   MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKE 120
           DDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKE
Sbjct: 61  DDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKE 120

Query: 121 SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG
Sbjct: 121 SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG 180

Query: 181 SGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240
           SGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKM 360

Query: 361 ERQNTLAREHTEEYKAALQRAVTLSVPHAYALSPYGTFSETVEGEEEKEEESPASSGGSS 420
           ERQNTLAREHTEEYKAALQRAVTL+VPHAY LSPYGTFSETVEGEEEKEEESPASSGGSS
Sbjct: 361 ERQNTLAREHTEEYKAALQRAVTLAVPHAYTLSPYGTFSETVEGEEEKEEESPASSGGSS 420

Query: 421 RKRKETWDELIERLYDKDDSRHNKNESRYTVLKKSFSSI 459
           RKRKETWDELIERLYDKDDS H+KNESRYTVLKKSFSSI
Sbjct: 421 RKRKETWDELIERLYDKDDSIHDKNESRYTVLKKSFSSI 459

BLAST of Cp4.1LG13g02550 vs. NCBI nr
Match: XP_022972992.1 (uncharacterized protein LOC111471517 [Cucurbita maxima] >XP_022972993.1 uncharacterized protein LOC111471517 [Cucurbita maxima] >XP_022972994.1 uncharacterized protein LOC111471517 [Cucurbita maxima] >XP_022972995.1 uncharacterized protein LOC111471517 [Cucurbita maxima])

HSP 1 Score: 923 bits (2386), Expect = 0.0
Identity = 450/459 (98.04%), Postives = 454/459 (98.91%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE 60
           MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFAT DEFEPIPRICRYILAVYE
Sbjct: 1   MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATTDEFEPIPRICRYILAVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKE 120
           DDIRKPLWEPVGGYGINPDWL+LKKTYKDTRGRAPPYILYLDHDH DIVLAIRGLNLAKE
Sbjct: 61  DDIRKPLWEPVGGYGINPDWLLLKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNLAKE 120

Query: 121 SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG
Sbjct: 121 SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG 180

Query: 181 SGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240
           SGVAAMLTLVVVQNH+KLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVVQNHKKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVM IPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMGIPPQQKM 360

Query: 361 ERQNTLAREHTEEYKAALQRAVTLSVPHAYALSPYGTFSETVEGEEEKEEESPASSGGSS 420
           ERQNTLAREHTEEYKAALQRAVTL+VPHAY LSPYGTFSETVEGEEEKEEESPASSGGS 
Sbjct: 361 ERQNTLAREHTEEYKAALQRAVTLAVPHAYTLSPYGTFSETVEGEEEKEEESPASSGGSL 420

Query: 421 RKRKETWDELIERLYDKDDSRHNKNESRYTVLKKSFSSI 459
           RKRKETWDELIERLYDKDDSRH+KNESRYTVLKKSFSSI
Sbjct: 421 RKRKETWDELIERLYDKDDSRHDKNESRYTVLKKSFSSI 459

BLAST of Cp4.1LG13g02550 vs. NCBI nr
Match: XP_004152416.1 (uncharacterized protein LOC101207943 [Cucumis sativus] >XP_011654833.1 uncharacterized protein LOC101207943 [Cucumis sativus] >KGN50301.1 hypothetical protein Csa_000149 [Cucumis sativus])

HSP 1 Score: 856 bits (2211), Expect = 2.23e-312
Identity = 417/459 (90.85%), Postives = 437/459 (95.21%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE 60
           MSI+CGVPILECVCCLGCARWVWKRCLHTAGHDSE WGFAT DEFEPIPRICRYIL+VYE
Sbjct: 1   MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKE 120
           DDIRKPLWEPVGGYGINPDWL++KKTYKDTRGRAPPYILYLDH HADIVLAIRGLN+AKE
Sbjct: 61  DDIRKPLWEPVGGYGINPDWLLMKKTYKDTRGRAPPYILYLDHVHADIVLAIRGLNMAKE 120

Query: 121 SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDN+LGK KFDGGYVHNGLLKAAGWVLD+ENETLKDLVKKYPDYTLTFAGHSLG
Sbjct: 121 SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180

Query: 181 SGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240
           SGVAAMLTL+VVQN EKLENIDRKRIRC+AIAPARCMSLNLAVRYADVINSVVLQDDFLP
Sbjct: 181 SGVAAMLTLLVVQNREKLENIDRKRIRCFAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLFCLPCLLCL C+RDTC+SE+KMLKDPRRLY PGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELM +D+K+MEIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMRKDDKIMEIPPQQKM 360

Query: 361 ERQNTLAREHTEEYKAALQRAVTLSVPHAYALSPYGTFSETVEGEEEKEEESPASSGGSS 420
           ERQ TLAREH+EEYKAALQRAVTL+VPHAYA+SPYGTFS+T EGEEEK   SPASSGGSS
Sbjct: 361 ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEK---SPASSGGSS 420

Query: 421 RKRKETWDELIERLYDKDDSRHNKNESRYTVLKKSFSSI 459
           R+RKETWDELIERLYDKDDSRH       TVLKKS S I
Sbjct: 421 RRRKETWDELIERLYDKDDSRH-------TVLKKSLSCI 449

BLAST of Cp4.1LG13g02550 vs. ExPASy TrEMBL
Match: A0A6J1E2T3 (uncharacterized protein LOC111430275 OS=Cucurbita moschata OX=3662 GN=LOC111430275 PE=4 SV=1)

HSP 1 Score: 934 bits (2413), Expect = 0.0
Identity = 456/459 (99.35%), Postives = 458/459 (99.78%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE 60
           MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE
Sbjct: 1   MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKE 120
           DDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKE
Sbjct: 61  DDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKE 120

Query: 121 SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG
Sbjct: 121 SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG 180

Query: 181 SGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240
           SGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKM 360

Query: 361 ERQNTLAREHTEEYKAALQRAVTLSVPHAYALSPYGTFSETVEGEEEKEEESPASSGGSS 420
           ERQNTLAREHTEEYKAALQRAVTL+VPHAY LSPYGTFSETVEGEEEKEEESPASSGGSS
Sbjct: 361 ERQNTLAREHTEEYKAALQRAVTLAVPHAYTLSPYGTFSETVEGEEEKEEESPASSGGSS 420

Query: 421 RKRKETWDELIERLYDKDDSRHNKNESRYTVLKKSFSSI 459
           RKRKETWDELIERLYDKDDSRH+KNESRYTVLKKSFSSI
Sbjct: 421 RKRKETWDELIERLYDKDDSRHDKNESRYTVLKKSFSSI 459

BLAST of Cp4.1LG13g02550 vs. ExPASy TrEMBL
Match: A0A6J1I6B2 (uncharacterized protein LOC111471517 OS=Cucurbita maxima OX=3661 GN=LOC111471517 PE=4 SV=1)

HSP 1 Score: 923 bits (2386), Expect = 0.0
Identity = 450/459 (98.04%), Postives = 454/459 (98.91%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE 60
           MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFAT DEFEPIPRICRYILAVYE
Sbjct: 1   MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATTDEFEPIPRICRYILAVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKE 120
           DDIRKPLWEPVGGYGINPDWL+LKKTYKDTRGRAPPYILYLDHDH DIVLAIRGLNLAKE
Sbjct: 61  DDIRKPLWEPVGGYGINPDWLLLKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNLAKE 120

Query: 121 SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG
Sbjct: 121 SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG 180

Query: 181 SGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240
           SGVAAMLTLVVVQNH+KLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVVQNHKKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVM IPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMGIPPQQKM 360

Query: 361 ERQNTLAREHTEEYKAALQRAVTLSVPHAYALSPYGTFSETVEGEEEKEEESPASSGGSS 420
           ERQNTLAREHTEEYKAALQRAVTL+VPHAY LSPYGTFSETVEGEEEKEEESPASSGGS 
Sbjct: 361 ERQNTLAREHTEEYKAALQRAVTLAVPHAYTLSPYGTFSETVEGEEEKEEESPASSGGSL 420

Query: 421 RKRKETWDELIERLYDKDDSRHNKNESRYTVLKKSFSSI 459
           RKRKETWDELIERLYDKDDSRH+KNESRYTVLKKSFSSI
Sbjct: 421 RKRKETWDELIERLYDKDDSRHDKNESRYTVLKKSFSSI 459

BLAST of Cp4.1LG13g02550 vs. ExPASy TrEMBL
Match: A0A0A0KRF0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G166450 PE=4 SV=1)

HSP 1 Score: 856 bits (2211), Expect = 1.08e-312
Identity = 417/459 (90.85%), Postives = 437/459 (95.21%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE 60
           MSI+CGVPILECVCCLGCARWVWKRCLHTAGHDSE WGFAT DEFEPIPRICRYIL+VYE
Sbjct: 1   MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKE 120
           DDIRKPLWEPVGGYGINPDWL++KKTYKDTRGRAPPYILYLDH HADIVLAIRGLN+AKE
Sbjct: 61  DDIRKPLWEPVGGYGINPDWLLMKKTYKDTRGRAPPYILYLDHVHADIVLAIRGLNMAKE 120

Query: 121 SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDN+LGK KFDGGYVHNGLLKAAGWVLD+ENETLKDLVKKYPDYTLTFAGHSLG
Sbjct: 121 SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180

Query: 181 SGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240
           SGVAAMLTL+VVQN EKLENIDRKRIRC+AIAPARCMSLNLAVRYADVINSVVLQDDFLP
Sbjct: 181 SGVAAMLTLLVVQNREKLENIDRKRIRCFAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLFCLPCLLCL C+RDTC+SE+KMLKDPRRLY PGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELM +D+K+MEIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMRKDDKIMEIPPQQKM 360

Query: 361 ERQNTLAREHTEEYKAALQRAVTLSVPHAYALSPYGTFSETVEGEEEKEEESPASSGGSS 420
           ERQ TLAREH+EEYKAALQRAVTL+VPHAYA+SPYGTFS+T EGEEEK   SPASSGGSS
Sbjct: 361 ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEK---SPASSGGSS 420

Query: 421 RKRKETWDELIERLYDKDDSRHNKNESRYTVLKKSFSSI 459
           R+RKETWDELIERLYDKDDSRH       TVLKKS S I
Sbjct: 421 RRRKETWDELIERLYDKDDSRH-------TVLKKSLSCI 449

BLAST of Cp4.1LG13g02550 vs. ExPASy TrEMBL
Match: A0A1S3ASL8 (uncharacterized protein LOC103482554 OS=Cucumis melo OX=3656 GN=LOC103482554 PE=4 SV=1)

HSP 1 Score: 854 bits (2207), Expect = 4.39e-312
Identity = 418/459 (91.07%), Postives = 435/459 (94.77%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE 60
           MSI+CGVPILECVCCLGCARWVWKRCLHTAGHDSE WGFAT DEFEPIPRICRYIL+VYE
Sbjct: 1   MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKE 120
           DDIRKPLWEPVGGYGINPDWLI+KKTYKDTRGRAPPYILYLDHDHADIVLAIRGLN+ KE
Sbjct: 61  DDIRKPLWEPVGGYGINPDWLIMKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNMVKE 120

Query: 121 SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDN+LGK KFDGGYVHNGLLKAAGWVLD+ENETLKDLVKKYPDYTLTF GHSLG
Sbjct: 121 SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFTGHSLG 180

Query: 181 SGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240
           SGVAAMLTLVVVQN EKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVVQNREKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLFCLPCLLCL C+RDTC+SE+KMLKDPRRLY PGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAK ALELM +D+K+MEIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKRALELMRKDDKIMEIPPQQKM 360

Query: 361 ERQNTLAREHTEEYKAALQRAVTLSVPHAYALSPYGTFSETVEGEEEKEEESPASSGGSS 420
           ERQ TLAREH+EEYKAALQRAVTL+VPHAYA+SPYGTFS+T EGEEEK   SPASSGGSS
Sbjct: 361 ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEK---SPASSGGSS 420

Query: 421 RKRKETWDELIERLYDKDDSRHNKNESRYTVLKKSFSSI 459
           R+RKETWDELIERLYDKDDSRH       TVLKKS S I
Sbjct: 421 RRRKETWDELIERLYDKDDSRH-------TVLKKSLSCI 449

BLAST of Cp4.1LG13g02550 vs. ExPASy TrEMBL
Match: A0A5A7TNW3 (Alpha/beta-Hydrolases superfamily protein isoform 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1167G00050 PE=4 SV=1)

HSP 1 Score: 854 bits (2207), Expect = 4.39e-312
Identity = 418/459 (91.07%), Postives = 435/459 (94.77%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE 60
           MSI+CGVPILECVCCLGCARWVWKRCLHTAGHDSE WGFAT DEFEPIPRICRYIL+VYE
Sbjct: 1   MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKE 120
           DDIRKPLWEPVGGYGINPDWLI+KKTYKDTRGRAPPYILYLDHDHADIVLAIRGLN+ KE
Sbjct: 61  DDIRKPLWEPVGGYGINPDWLIMKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNMVKE 120

Query: 121 SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDN+LGK KFDGGYVHNGLLKAAGWVLD+ENETLKDLVKKYPDYTLTF GHSLG
Sbjct: 121 SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFTGHSLG 180

Query: 181 SGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240
           SGVAAMLTLVVVQN EKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVVQNREKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLFCLPCLLCL C+RDTC+SE+KMLKDPRRLY PGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAK ALELM +D+K+MEIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKRALELMRKDDKIMEIPPQQKM 360

Query: 361 ERQNTLAREHTEEYKAALQRAVTLSVPHAYALSPYGTFSETVEGEEEKEEESPASSGGSS 420
           ERQ TLAREH+EEYKAALQRAVTL+VPHAYA+SPYGTFS+T EGEEEK   SPASSGGSS
Sbjct: 361 ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEK---SPASSGGSS 420

Query: 421 RKRKETWDELIERLYDKDDSRHNKNESRYTVLKKSFSSI 459
           R+RKETWDELIERLYDKDDSRH       TVLKKS S I
Sbjct: 421 RRRKETWDELIERLYDKDDSRH-------TVLKKSLSCI 449

BLAST of Cp4.1LG13g02550 vs. TAIR 10
Match: AT3G49050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 690.6 bits (1781), Expect = 8.3e-199
Identity = 339/465 (72.90%), Postives = 392/465 (84.30%), Query Frame = 0

Query: 1   MSIICG-VPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVY 60
           MSI+CG  P+LECV CLGCARW +KRCL+TAGHDSE WG AT DEFEP+PR CRYILAVY
Sbjct: 1   MSILCGCCPLLECVYCLGCARWGYKRCLYTAGHDSEDWGLATTDEFEPVPRFCRYILAVY 60

Query: 61  EDDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAK 120
           EDDIR PLWEP  GYGINPDWL+LKKTY+DT+GRAP YILYLDH H DIV+AIRGLNLAK
Sbjct: 61  EDDIRNPLWEPPEGYGINPDWLLLKKTYEDTQGRAPAYILYLDHVHQDIVVAIRGLNLAK 120

Query: 121 ESDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSL 180
           ESDYA+LLDNKLG+ KFDGGYVHNGL+K+AG+VLD E + LK+LVKKYP YTLTFAGHSL
Sbjct: 121 ESDYAMLLDNKLGERKFDGGYVHNGLVKSAGYVLDEECKVLKELVKKYPSYTLTFAGHSL 180

Query: 181 GSGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFL 240
           GSGVA ML L+VV++ E+L NIDRKR+RC+AIAPARCMSLNLAVRYADVINSV+LQDDFL
Sbjct: 181 GSGVATMLALLVVRHPERLGNIDRKRVRCFAIAPARCMSLNLAVRYADVINSVILQDDFL 240

Query: 241 PRTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRC 300
           PRTATPLEDIFKS+FCLPCLLC+ C++DTC+ E KMLKDPRRLY PGR+YHIVERKP R 
Sbjct: 241 PRTATPLEDIFKSVFCLPCLLCIRCMKDTCVPEQKMLKDPRRLYAPGRMYHIVERKPCRL 300

Query: 301 GRFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQK 360
           GR+PPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIE+EA+ AL LM+E+ K MEIP +Q+
Sbjct: 301 GRYPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEREAQRALNLMMENEKKMEIPEKQR 360

Query: 361 MERQNTLAREHTEEYKAALQRAVTLSVPHAYALS-PYGTFSETVEGE--------EEKEE 420
           MERQ +LAREH  EY+AAL+RAVTL VPHA +++  YGTF +T E E        EE+EE
Sbjct: 361 MERQESLAREHNLEYRAALRRAVTLDVPHAESMAYEYGTFDKTQEDETEEEEVETEEEEE 420

Query: 421 ESPA-------SSGGSS--------RKRKETWDELIERLYDKDDS 441
           ++ +       SS  SS        R R+ +WDELIE L+++D+S
Sbjct: 421 DTDSIAPMVGESSSSSSVKPTYRIRRNRRVSWDELIEHLFERDES 465

BLAST of Cp4.1LG13g02550 vs. TAIR 10
Match: AT4G00500.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 587.4 bits (1513), Expect = 9.8e-168
Identity = 282/456 (61.84%), Postives = 358/456 (78.51%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE 60
           MSI+C VP+LECV CLGC  W+WK+CL++AGH+SE WG AT+DEFEPIPRICR ILAVYE
Sbjct: 1   MSILCCVPVLECVYCLGCTHWLWKKCLYSAGHESENWGLATSDEFEPIPRICRLILAVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKE 120
           +++  P+W P  GYGI+P+ +ILKK Y  T GR  PY++YLDH++ D+VLAIRGLNLAKE
Sbjct: 61  ENLHDPMWAPPDGYGIDPNHVILKKDYDQTEGRVTPYMIYLDHENGDVVLAIRGLNLAKE 120

Query: 121 SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG 180
            DYAVLLDNKLG+ KFDGGYVHNGLLKAA WV + E+  L++L++  P Y+LTF GHSLG
Sbjct: 121 CDYAVLLDNKLGQTKFDGGYVHNGLLKAAMWVFEEEHVVLRELLEANPSYSLTFVGHSLG 180

Query: 181 SGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240
           +GV ++L L V+QN  +L NI+RKRIRC+AIAP RCMSL+LAV YADVINSVVLQDDFLP
Sbjct: 181 AGVVSLLVLFVIQNRVRLGNIERKRIRCFAIAPPRCMSLHLAVTYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG 300
           RT T LE++FKS+ CLPCLLCL CL+DT   E++ LKD RRLY PGRLYHIV RKP R G
Sbjct: 241 RTTTALENVFKSIICLPCLLCLTCLKDTFTFEERKLKDARRLYAPGRLYHIVVRKPLRLG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKM 360
           R+PPVV+TAVPVDGRFE IVLSCNAT+DHAIIWIE+E++ AL+LM+E+++VM+IP +QK+
Sbjct: 301 RYPPVVRTAVPVDGRFEQIVLSCNATADHAIIWIERESQRALDLMVEEDQVMQIPVEQKI 360

Query: 361 ERQNTLAREHTEEYKAALQRAVTLSVPHAYALSPYGTFSETVEGEEEKEEESPAS-SGGS 420
            RQ ++  +H EEY+AA+ +A +L++P + + S YGTF +T EGE         S SG S
Sbjct: 361 VRQKSIVEDHDEEYRAAIMKAASLNIPMSPSPS-YGTFHDTEEGESSAGSGMEGSPSGWS 420

Query: 421 SRKRKETWDELIERLYD-KDDSRHN--KNESRYTVL 453
            +  +  WD+ I+  +   D+S H   KN+    +L
Sbjct: 421 FKGMRRKWDQFIDCHFPVNDNSEHMIFKNQESQALL 455

BLAST of Cp4.1LG13g02550 vs. TAIR 10
Match: AT4G00500.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 587.4 bits (1513), Expect = 9.8e-168
Identity = 282/456 (61.84%), Postives = 358/456 (78.51%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE 60
           MSI+C VP+LECV CLGC  W+WK+CL++AGH+SE WG AT+DEFEPIPRICR ILAVYE
Sbjct: 1   MSILCCVPVLECVYCLGCTHWLWKKCLYSAGHESENWGLATSDEFEPIPRICRLILAVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKE 120
           +++  P+W P  GYGI+P+ +ILKK Y  T GR  PY++YLDH++ D+VLAIRGLNLAKE
Sbjct: 61  ENLHDPMWAPPDGYGIDPNHVILKKDYDQTEGRVTPYMIYLDHENGDVVLAIRGLNLAKE 120

Query: 121 SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG 180
            DYAVLLDNKLG+ KFDGGYVHNGLLKAA WV + E+  L++L++  P Y+LTF GHSLG
Sbjct: 121 CDYAVLLDNKLGQTKFDGGYVHNGLLKAAMWVFEEEHVVLRELLEANPSYSLTFVGHSLG 180

Query: 181 SGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240
           +GV ++L L V+QN  +L NI+RKRIRC+AIAP RCMSL+LAV YADVINSVVLQDDFLP
Sbjct: 181 AGVVSLLVLFVIQNRVRLGNIERKRIRCFAIAPPRCMSLHLAVTYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG 300
           RT T LE++FKS+ CLPCLLCL CL+DT   E++ LKD RRLY PGRLYHIV RKP R G
Sbjct: 241 RTTTALENVFKSIICLPCLLCLTCLKDTFTFEERKLKDARRLYAPGRLYHIVVRKPLRLG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKM 360
           R+PPVV+TAVPVDGRFE IVLSCNAT+DHAIIWIE+E++ AL+LM+E+++VM+IP +QK+
Sbjct: 301 RYPPVVRTAVPVDGRFEQIVLSCNATADHAIIWIERESQRALDLMVEEDQVMQIPVEQKI 360

Query: 361 ERQNTLAREHTEEYKAALQRAVTLSVPHAYALSPYGTFSETVEGEEEKEEESPAS-SGGS 420
            RQ ++  +H EEY+AA+ +A +L++P + + S YGTF +T EGE         S SG S
Sbjct: 361 VRQKSIVEDHDEEYRAAIMKAASLNIPMSPSPS-YGTFHDTEEGESSAGSGMEGSPSGWS 420

Query: 421 SRKRKETWDELIERLYD-KDDSRHN--KNESRYTVL 453
            +  +  WD+ I+  +   D+S H   KN+    +L
Sbjct: 421 FKGMRRKWDQFIDCHFPVNDNSEHMIFKNQESQALL 455

BLAST of Cp4.1LG13g02550 vs. TAIR 10
Match: AT5G37710.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 507.7 bits (1306), Expect = 9.9e-144
Identity = 256/445 (57.53%), Postives = 330/445 (74.16%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE 60
           MS+ CG   LECV C+G +RW WKRC H    DS TW  AT +EFEPIPRI R ILAVYE
Sbjct: 1   MSVACG---LECVFCVGFSRWAWKRCTHVGSDDSATWTSATPEEFEPIPRISRVILAVYE 60

Query: 61  DDIRKPLWEP-VGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAK 120
            D+R P   P +G + +NP+W+I + T++ T+GR+PPYI+Y+DHDH +IVLAIRGLNLAK
Sbjct: 61  PDLRNPKISPSLGTFDLNPEWVIKRVTHEKTQGRSPPYIIYIDHDHREIVLAIRGLNLAK 120

Query: 121 ESDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETL-KDLVKKYPDYTLTFAGHS 180
           ESDY +LLDNKLG+    GGYVH GLLK+A WVL+ E+ETL +   +   +Y L FAGHS
Sbjct: 121 ESDYKILLDNKLGQKMLGGGYVHRGLLKSAAWVLNQESETLWRVWEENGREYDLVFAGHS 180

Query: 181 LGSGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDF 240
           LGSGVAA++ ++VV     + +I R ++RC+A+APARCMSLNLAV+YADVI+SV+LQDDF
Sbjct: 181 LGSGVAALMAVLVVNTPAMIGDIPRNKVRCFALAPARCMSLNLAVKYADVISSVILQDDF 240

Query: 241 LPRTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFR 300
           LPRTATPLEDIFKS+FCLPCLL L CLRDT I E + L+DPRRLY PGR+YHIVERK   
Sbjct: 241 LPRTATPLEDIFKSVFCLPCLLFLVCLRDTFIPEGRKLRDPRRLYAPGRIYHIVERK--- 300

Query: 301 CGRFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLE---DNKVMEIP 360
             RFPP V+TA+PVDGRFEHIVLS NATSDHAI+WIE+EA+ AL+++ E   +  V   P
Sbjct: 301 FCRFPPEVRTAIPVDGRFEHIVLSSNATSDHAILWIEREAEKALQILREKSSETVVTMAP 360

Query: 361 PQQKMERQNTLAREHTEEYKAALQRAVTLSVPHAYALSPYGTFSETVEGEEEKEEESPAS 420
            +++MER +TL +EH    K AL+RAV+L++PHA            V   EE+EE +   
Sbjct: 361 KEKRMERLSTLEKEH----KDALERAVSLNIPHA------------VSTAEEEEECNNGE 420

Query: 421 SGGSSRKRKETWDELIERLYDKDDS 441
           +    + +K+ WDE++++L+ + +S
Sbjct: 421 ASAELKTKKKNWDEVVDKLFHRSNS 423

BLAST of Cp4.1LG13g02550 vs. TAIR 10
Match: AT2G42450.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 83.2 bits (204), Expect = 6.0e-16
Identity = 44/157 (28.03%), Postives = 80/157 (50.96%), Query Frame = 0

Query: 85  KTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKESDYAVLLDNKLGKMKFDGGYVHNG 144
           K  KD+    P Y + +DH    +V  IRG +   +     ++ +   ++ F+G   H G
Sbjct: 219 KFVKDSSVMRPGYYIGVDHRRKLVVFGIRGTHTIYDL-ITDIVSSSDEEVTFEGYSTHFG 278

Query: 145 LLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLGSGVAAMLTLVVVQNHEKLENIDRK 204
             +AA W L+ E +T++  + KY  Y L   GHSLG  +A+++ +++ +   +    D +
Sbjct: 279 TAEAARWFLNHELQTIRRCLAKYEGYKLRLVGHSLGGAIASLMAIMLKKMPREELGFDAE 338

Query: 205 RIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLPR 242
            I     A   C+S  LA   ++ + ++V+QDD +PR
Sbjct: 339 IISAVGYATPPCVSKELAENCSEFVTTIVMQDDIIPR 374

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8NCG75.9e-0828.13Diacylglycerol lipase-beta OS=Homo sapiens OX=9606 GN=DAGLB PE=1 SV=2[more]
P0C1S95.9e-0826.88Diacylglycerol lipase-beta OS=Rattus norvegicus OX=10116 GN=Daglb PE=1 SV=1[more]
Q91WC91.3e-0726.88Diacylglycerol lipase-beta OS=Mus musculus OX=10090 GN=Daglb PE=1 SV=2[more]
Q6WQJ15.5e-0622.73Diacylglycerol lipase-alpha OS=Mus musculus OX=10090 GN=Dagla PE=1 SV=2[more]
Q5YLM15.5e-0622.73Diacylglycerol lipase-alpha OS=Rattus norvegicus OX=10116 GN=Dagla PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_023550858.10.0100.00uncharacterized protein LOC111808873 [Cucurbita pepo subsp. pepo] >XP_023550859.... [more]
XP_022922227.10.099.35uncharacterized protein LOC111430275 [Cucurbita moschata] >XP_022922228.1 unchar... [more]
KAG6579384.10.099.13hypothetical protein SDJN03_23832, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022972992.10.098.04uncharacterized protein LOC111471517 [Cucurbita maxima] >XP_022972993.1 uncharac... [more]
XP_004152416.12.23e-31290.85uncharacterized protein LOC101207943 [Cucumis sativus] >XP_011654833.1 uncharact... [more]
Match NameE-valueIdentityDescription
A0A6J1E2T30.099.35uncharacterized protein LOC111430275 OS=Cucurbita moschata OX=3662 GN=LOC1114302... [more]
A0A6J1I6B20.098.04uncharacterized protein LOC111471517 OS=Cucurbita maxima OX=3661 GN=LOC111471517... [more]
A0A0A0KRF01.08e-31290.85Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G166450 PE=4 SV=1[more]
A0A1S3ASL84.39e-31291.07uncharacterized protein LOC103482554 OS=Cucumis melo OX=3656 GN=LOC103482554 PE=... [more]
A0A5A7TNW34.39e-31291.07Alpha/beta-Hydrolases superfamily protein isoform 1 OS=Cucumis melo var. makuwa ... [more]
Match NameE-valueIdentityDescription
AT3G49050.18.3e-19972.90alpha/beta-Hydrolases superfamily protein [more]
AT4G00500.19.8e-16861.84alpha/beta-Hydrolases superfamily protein [more]
AT4G00500.29.8e-16861.84alpha/beta-Hydrolases superfamily protein [more]
AT5G37710.19.9e-14457.53alpha/beta-Hydrolases superfamily protein [more]
AT2G42450.16.0e-1628.03alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 360..380
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 411..425
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 400..425
NoneNo IPR availablePANTHERPTHR46398:SF4ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 1..443
NoneNo IPR availablePANTHERPTHR46398ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 1..443
NoneNo IPR availableCDDcd00519Lipase_3coord: 44..241
e-value: 1.43027E-30
score: 116.037
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 39..252
e-value: 4.2E-27
score: 96.9
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 42..273
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 109..243
e-value: 2.2E-24
score: 85.9
IPR005592Mono-/di-acylglycerol lipase, N-terminalPFAMPF03893Lipase3_Ncoord: 10..73
e-value: 3.5E-20
score: 71.8

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG13g02550.1Cp4.1LG13g02550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016042 lipid catabolic process
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0016787 hydrolase activity