Cp4.1LG13g02110 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG13g02110
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	Cp4.1LG13: 1638605 .. 1639258 (+)
RNA-Seq Expression	Cp4.1LG13g02110
Synteny	Cp4.1LG13g02110

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGAATTTCCATTTTTCTCTTCCCAAATTCCTGTTTCTCCTCCTCCTCCTCCTCATAATTTCCGACCAAATTCATGTCTGCTCCGCCGCCGGAAGAACCCACAAATCCCCTGCTAGATTCAGAACAAACACAGAGTATGTGAGAACCTCGTGCAGTGCCACTTCATACCCTCGCCTCTGTTACAATTCACTCTCGGTGTACGCCGGCAAGATCAAGACCAACCCCAAGACGCTATCTCTCGCTGCCCTCCATGTCAATCTCGCCTCCGCCCGATCCACGGCTGTGGCAATGAGAAGACTAGCCAAAACCCGTGGACTACGACGGAGAGACGCGTCGGCAATCTCCGATTGCATAGAGGAGGTAGGCGACTCAGTGTTCGAGCTTCATAGGGCGATTCGCGAATTGTGTCGTCCTAGAGGGTATGATTTTGGGGCTTTGATTAGCGACATTGAGACATGGGTTAGCTCTGCTCTAACCGACGAAGAGACGTGTATGGAAGGGTTTGGCGGGCGGAGTGTTAGTAATAGGTTCAGTGTGAAGGCTAAGGTCCGACGGCACATTGTTAGAGTTGCGCATTTGACCAGCAATTCACTTGCTCTTATTAACACCTATGCTTCGTCGGCAGCGGCGGCCCAAGGCGTTCTGCCCTAG

mRNA sequence

ATGGGGAATTTCCATTTTTCTCTTCCCAAATTCCTGTTTCTCCTCCTCCTCCTCCTCATAATTTCCGACCAAATTCATGTCTGCTCCGCCGCCGGAAGAACCCACAAATCCCCTGCTAGATTCAGAACAAACACAGAGTATGTGAGAACCTCGTGCAGTGCCACTTCATACCCTCGCCTCTGTTACAATTCACTCTCGGTGTACGCCGGCAAGATCAAGACCAACCCCAAGACGCTATCTCTCGCTGCCCTCCATGTCAATCTCGCCTCCGCCCGATCCACGGCTGTGGCAATGAGAAGACTAGCCAAAACCCGTGGACTACGACGGAGAGACGCGTCGGCAATCTCCGATTGCATAGAGGAGGTAGGCGACTCAGTGTTCGAGCTTCATAGGGCGATTCGCGAATTGTGTCGTCCTAGAGGGTATGATTTTGGGGCTTTGATTAGCGACATTGAGACATGGGTTAGCTCTGCTCTAACCGACGAAGAGACGTGTATGGAAGGGTTTGGCGGGCGGAGTGTTAGTAATAGGTTCAGTGTGAAGGCTAAGGTCCGACGGCACATTGTTAGAGTTGCGCATTTGACCAGCAATTCACTTGCTCTTATTAACACCTATGCTTCGTCGGCAGCGGCGGCCCAAGGCGTTCTGCCCTAG

Coding sequence (CDS)

ATGGGGAATTTCCATTTTTCTCTTCCCAAATTCCTGTTTCTCCTCCTCCTCCTCCTCATAATTTCCGACCAAATTCATGTCTGCTCCGCCGCCGGAAGAACCCACAAATCCCCTGCTAGATTCAGAACAAACACAGAGTATGTGAGAACCTCGTGCAGTGCCACTTCATACCCTCGCCTCTGTTACAATTCACTCTCGGTGTACGCCGGCAAGATCAAGACCAACCCCAAGACGCTATCTCTCGCTGCCCTCCATGTCAATCTCGCCTCCGCCCGATCCACGGCTGTGGCAATGAGAAGACTAGCCAAAACCCGTGGACTACGACGGAGAGACGCGTCGGCAATCTCCGATTGCATAGAGGAGGTAGGCGACTCAGTGTTCGAGCTTCATAGGGCGATTCGCGAATTGTGTCGTCCTAGAGGGTATGATTTTGGGGCTTTGATTAGCGACATTGAGACATGGGTTAGCTCTGCTCTAACCGACGAAGAGACGTGTATGGAAGGGTTTGGCGGGCGGAGTGTTAGTAATAGGTTCAGTGTGAAGGCTAAGGTCCGACGGCACATTGTTAGAGTTGCGCATTTGACCAGCAATTCACTTGCTCTTATTAACACCTATGCTTCGTCGGCAGCGGCGGCCCAAGGCGTTCTGCCCTAG

Protein sequence

MGNFHFSLPKFLFLLLLLLIISDQIHVCSAAGRTHKSPARFRTNTEYVRTSCSATSYPRLCYNSLSVYAGKIKTNPKTLSLAALHVNLASARSTAVAMRRLAKTRGLRRRDASAISDCIEEVGDSVFELHRAIRELCRPRGYDFGALISDIETWVSSALTDEETCMEGFGGRSVSNRFSVKAKVRRHIVRVAHLTSNSLALINTYASSAAAAQGVLP

Homology

[Close]

BLAST of Cp4.1LG13g02110 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 5.4e-36
Identity = 81/170 (47.65%), Postives = 120/170 (70.59%), Query Frame = 0


Query: 42  RTNTEYVRTSCSATSYPRLCYNSLSVYAGKIKTNPKTLSLAALHVNLASARSTAVAMRRL 101
           +TN +Y++TSC+ T Y  +CYNSLS YA  I++NP+ L++ AL++ L+SA+S +  ++ +
Sbjct: 142 QTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKFVKNI 201

Query: 102 AKTRGLRRRDASAISDCIEEVGDSVFELHRAIRELCRPRGYD---FGALISDIETWVSSA 161
           +   GL R +  A++DC+EE+GDSV  L  +IREL      D   F  ++SD+ETWVS+A
Sbjct: 202 SHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETWVSAA 261

Query: 162 LTDEETCMEGFGGRSVSNRFSVKAKVRRHIVRVAHLTSNSLALINTYASS 209
           LT+++TCM+GF       + +VK  VRRH+V VA LTSN+LALIN YAS+
Sbjct: 262 LTNDDTCMDGFS----LVKTAVKDLVRRHVVEVARLTSNALALINMYAST 307

[Close]

BLAST of Cp4.1LG13g02110 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.2e-32
Identity = 72/165 (43.64%), Postives = 110/165 (66.67%), Query Frame = 0


Query: 46  EYVRTSCSATSYPRLCYNSLSVYAGKIKTNPKTLSLAALHVNLASARSTAVAMRRLAKTR 105
           ++++TSC+ T+YP +C  SLS YA  I+ NP+ L+  AL V+L   +     M+RL K +
Sbjct: 27  QFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQVSLTRTQQAQTFMKRLNKFK 86

Query: 106 GLRRRDASAISDCIEEVGDSVFELHRA---IRELCRPRGYDFGALISDIETWVSSALTDE 165
           GL+ R  +AI DC+EEV DS+  + R+   ++ L   +G DF   +S++ETWVS+ALTDE
Sbjct: 87  GLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGNDFTFRMSNVETWVSAALTDE 146

Query: 166 ETCMEGFGGRSVSNRFSVKAKVRRHIVRVAHLTSNSLALINTYAS 208
            TCM+GF G+ +  +  +K  VR  +V VA +TSN+LAL+N +A+
Sbjct: 147 TTCMDGFAGKGMDGK--IKESVRAQVVAVARVTSNALALVNNFAA 189

[Close]

BLAST of Cp4.1LG13g02110 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 2.8e-32
Identity = 72/168 (42.86%), Postives = 110/168 (65.48%), Query Frame = 0


Query: 44  NTEYVRTSCSATSYPRLCYNSLSVYAGKIKTNPKTLSLAALHVNLASARSTAVAMRRLAK 103
           +TE+++ SC  TSYP  C+ SLS YA +IK  P+ L+  AL V++A A+S    +  +  
Sbjct: 34  DTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALAVSIARAKSAKTYVSEMTD 93

Query: 104 TRGLRRRDASAISDCIEEVGDSVFELHRAIRELCR----PRGYDFGALISDIETWVSSAL 163
            +G+ +R   A++DC+EE+GD+V  L  +++EL        G DF   +S++ TW S+AL
Sbjct: 94  YKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGDSGEDFWFCLSNVRTWTSAAL 153

Query: 164 TDEETCMEGFGGRSVSNRFSVKAKVRRHIVRVAHLTSNSLALINTYAS 208
           TDE  CM+GFGG++++    +K+ +R HIV VA  TSN+LALIN +AS
Sbjct: 154 TDETACMDGFGGKAMAG--ELKSLIRTHIVSVAEETSNALALINDFAS 199

[Close]

BLAST of Cp4.1LG13g02110 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.2e-27
Identity = 77/203 (37.93%), Postives = 121/203 (59.61%), Query Frame = 0


Query: 8   LPKFLFLLLLLLIISDQIHVCSAAGRTHKSPARFRTNTEYVRTSCSATSYPRLCYNSLSV 67
           + K +F  L L ++S  I   +A+    ++    +    +++ SC AT+YP +C NSL+ 
Sbjct: 1   MAKQIFYTLFLFLLSTAI--LTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTG 60

Query: 68  YAGKIKTNPKTLSLAALHVNLASARSTAVAMRRLAKTRGLRRRDASAISDCIEEVGDSVF 127
           YA  I+T+P+ L+  AL+V +  A+ST V + RL +   L++R+  A+ DCIEE+ D+V 
Sbjct: 61  YANSIQTSPRRLAETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVD 120

Query: 128 ELHRAIRE--LC-RPRGYD-FGALISDIETWVSSALTDEETCMEGFGGRSVSNRFSVKAK 187
            L  +I E  +C   +G D F   +S+ +TW S+ALT+  TC +GF GR +  R  VK  
Sbjct: 121 RLTMSIHEVKMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGR--VKNS 180

Query: 188 VRRHIVRVAHLTSNSLALINTYA 207
           VR  I+ +   TSN+LALIN +A
Sbjct: 181 VRARILNLGRGTSNALALINAFA 199

[Close]

BLAST of Cp4.1LG13g02110 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.3e-26
Identity = 79/205 (38.54%), Postives = 126/205 (61.46%), Query Frame = 0


Query: 8   LPKFLFLLLLLLIISDQIHVCSAAGRTHKSPARFRTNTEYVRTSCSATSYPRLCYNSLSV 67
           L   +FL++LL I   Q    S++   ++S      + +++ +SC  T YP LC ++LS 
Sbjct: 3   LKNTIFLVILLSITILQ----SSSATPNRS-----ESDQFIVSSCQTTQYPSLCVHTLSA 62

Query: 68  YAGKIK-TNPKTLSLAALHVNLASARSTAVAMRRLAK-TRGLRRRDASAISDCIEEVGDS 127
           YA KI+  N + L+  AL ++LA A+S  + + +L K T   +RR+  AI DCIE +G+S
Sbjct: 63  YATKIRHNNDQDLAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNS 122

Query: 128 VFELHRAIRELCRP----RGYDFGALISDIETWVSSALTDEETCMEGFGGRSVSNRFSVK 187
           V  L ++++EL R        DF   +S+++TWVS+ALTDE TC++GF  R++  +  VK
Sbjct: 123 VDRLAQSVKELARAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGK--VK 182

Query: 188 AKVRRHIVRVAHLTSNSLALINTYA 207
             +R  +V VA +TSN+LAL+N +A
Sbjct: 183 RLIRYKVVHVAQVTSNALALVNQFA 196

[Close]

BLAST of Cp4.1LG13g02110 vs. NCBI nr
Match: XP_023550543.1 (21 kDa protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 419 bits (1077), Expect = 1.61e-147
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0


Query: 1   MGNFHFSLPKFLFLLLLLLIISDQIHVCSAAGRTHKSPARFRTNTEYVRTSCSATSYPRL 60
           MGNFHFSLPKFLFLLLLLLIISDQIHVCSAAGRTHKSPARFRTNTEYVRTSCSATSYPRL
Sbjct: 1   MGNFHFSLPKFLFLLLLLLIISDQIHVCSAAGRTHKSPARFRTNTEYVRTSCSATSYPRL 60

Query: 61  CYNSLSVYAGKIKTNPKTLSLAALHVNLASARSTAVAMRRLAKTRGLRRRDASAISDCIE 120
           CYNSLSVYAGKIKTNPKTLSLAALHVNLASARSTAVAMRRLAKTRGLRRRDASAISDCIE
Sbjct: 61  CYNSLSVYAGKIKTNPKTLSLAALHVNLASARSTAVAMRRLAKTRGLRRRDASAISDCIE 120

Query: 121 EVGDSVFELHRAIRELCRPRGYDFGALISDIETWVSSALTDEETCMEGFGGRSVSNRFSV 180
           EVGDSVFELHRAIRELCRPRGYDFGALISDIETWVSSALTDEETCMEGFGGRSVSNRFSV
Sbjct: 121 EVGDSVFELHRAIRELCRPRGYDFGALISDIETWVSSALTDEETCMEGFGGRSVSNRFSV 180

Query: 181 KAKVRRHIVRVAHLTSNSLALINTYASSAAAAQGVLP 217
           KAKVRRHIVRVAHLTSNSLALINTYASSAAAAQGVLP
Sbjct: 181 KAKVRRHIVRVAHLTSNSLALINTYASSAAAAQGVLP 217

[Close]

BLAST of Cp4.1LG13g02110 vs. NCBI nr
Match: KAG6579432.1 (Pectinesterase inhibitor 10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 390 bits (1001), Expect = 7.67e-136
Identity = 207/223 (92.83%), Postives = 212/223 (95.07%), Query Frame = 0


Query: 1   MGNFHFSLPKFLFLLLLLL--IISDQIHVCSAAG----RTHKSPARFRTNTEYVRTSCSA 60
           M NFHFSLPKFLFLLLLLL  +ISDQIH+CSAA     RTH+SPAR RTNTEYVRTSCSA
Sbjct: 1   MENFHFSLPKFLFLLLLLLLLVISDQIHICSAAAAAAARTHRSPARIRTNTEYVRTSCSA 60

Query: 61  TSYPRLCYNSLSVYAGKIKTNPKTLSLAALHVNLASARSTAVAMRRLAKTRGLRRRDASA 120
           TSYPRLCYNSLSVYAGKIKTNPKTL+LAALHVNLASARSTAVAMRRLAKTRGLRRRDASA
Sbjct: 61  TSYPRLCYNSLSVYAGKIKTNPKTLALAALHVNLASARSTAVAMRRLAKTRGLRRRDASA 120

Query: 121 ISDCIEEVGDSVFELHRAIRELCRPRGYDFGALISDIETWVSSALTDEETCMEGFGGRSV 180
           ISDCIEEVGDSVFEL RAIRELCRPR YDFGALISDIETWVSSALTDEETCMEGFGGRSV
Sbjct: 121 ISDCIEEVGDSVFELQRAIRELCRPRVYDFGALISDIETWVSSALTDEETCMEGFGGRSV 180

Query: 181 SNRFSVKAKVRRHIVRVAHLTSNSLALINTYASSAAAAQGVLP 217
           +NRFSVKAKVRRHIVRVAHLTSNSLALINTYASSAAAAQGVLP
Sbjct: 181 NNRFSVKAKVRRHIVRVAHLTSNSLALINTYASSAAAAQGVLP 223

[Close]

BLAST of Cp4.1LG13g02110 vs. NCBI nr
Match: XP_022922163.1 (21 kDa protein-like [Cucurbita moschata])

HSP 1 Score: 389 bits (1000), Expect = 9.78e-136
Identity = 207/221 (93.67%), Postives = 211/221 (95.48%), Query Frame = 0


Query: 1   MGNFHFSLPKFLFLLLLLLIISDQIHVCSAAG----RTHKSPARFRTNTEYVRTSCSATS 60
           M NFHFSLPKFLFLLLLL IISDQIH+CSAA     RTH+SPAR RTNTEYVRTSCSATS
Sbjct: 1   MENFHFSLPKFLFLLLLL-IISDQIHICSAAAAAAARTHRSPARIRTNTEYVRTSCSATS 60

Query: 61  YPRLCYNSLSVYAGKIKTNPKTLSLAALHVNLASARSTAVAMRRLAKTRGLRRRDASAIS 120
           YPRLCYNSLSVYAGKIKTNPKTL+LAALHVNLASARSTAVAMRRLAKTRGLRRRDASAIS
Sbjct: 61  YPRLCYNSLSVYAGKIKTNPKTLALAALHVNLASARSTAVAMRRLAKTRGLRRRDASAIS 120

Query: 121 DCIEEVGDSVFELHRAIRELCRPRGYDFGALISDIETWVSSALTDEETCMEGFGGRSVSN 180
           DCIEEVGDSVFEL RAIRELCRPR YDFGALISDIETWVSSALTDEETCMEGFGGRSV+N
Sbjct: 121 DCIEEVGDSVFELQRAIRELCRPRVYDFGALISDIETWVSSALTDEETCMEGFGGRSVNN 180

Query: 181 RFSVKAKVRRHIVRVAHLTSNSLALINTYASSAAAAQGVLP 217
           RFSVKAKVRRHIVRVAHLTSNSLALINTYASSAAAAQGVLP
Sbjct: 181 RFSVKAKVRRHIVRVAHLTSNSLALINTYASSAAAAQGVLP 220

[Close]

BLAST of Cp4.1LG13g02110 vs. NCBI nr
Match: XP_022969879.1 (21 kDa protein-like [Cucurbita maxima])

HSP 1 Score: 385 bits (989), Expect = 4.17e-134
Identity = 206/219 (94.06%), Postives = 208/219 (94.98%), Query Frame = 0


Query: 1   MGNFHFSLPKFLFLLLLLLIISDQIHVCSAAG--RTHKSPARFRTNTEYVRTSCSATSYP 60
           M NFHFSLPKFLFLLLL  IISDQIH+CSAA   RTHKSPAR RTNTEYVRTSCSATSYP
Sbjct: 1   MENFHFSLPKFLFLLLL--IISDQIHICSAAAATRTHKSPARIRTNTEYVRTSCSATSYP 60

Query: 61  RLCYNSLSVYAGKIKTNPKTLSLAALHVNLASARSTAVAMRRLAKTRGLRRRDASAISDC 120
           RLCYNSLSVYAGKIKTNPKTL LAALHVNLASARSTAVAMRRLAKTRGLRRRDASAISDC
Sbjct: 61  RLCYNSLSVYAGKIKTNPKTLVLAALHVNLASARSTAVAMRRLAKTRGLRRRDASAISDC 120

Query: 121 IEEVGDSVFELHRAIRELCRPRGYDFGALISDIETWVSSALTDEETCMEGFGGRSVSNRF 180
           IEEVGDSVFEL RAIREL RPRGYDFGALISDIETWVSSALTDEETCMEGFGGRSV+NRF
Sbjct: 121 IEEVGDSVFELQRAIRELRRPRGYDFGALISDIETWVSSALTDEETCMEGFGGRSVNNRF 180

Query: 181 SVKAKVRRHIVRVAHLTSNSLALINTYASSAAAAQGVLP 217
           SVKA VRRHIVRVAHLTSNSLALINTYASSAAAAQGVLP
Sbjct: 181 SVKAMVRRHIVRVAHLTSNSLALINTYASSAAAAQGVLP 217

[Close]

BLAST of Cp4.1LG13g02110 vs. NCBI nr
Match: XP_038906530.1 (21 kDa protein-like [Benincasa hispida])

HSP 1 Score: 325 bits (832), Expect = 3.84e-110
Identity = 177/221 (80.09%), Postives = 190/221 (85.97%), Query Frame = 0


Query: 1   MGNFHF----SLPKFLFLLLLLLIISDQIHVCSAAGRTHKSPARFRTNTEYVRTSCSATS 60
           M N HF    + P FL LLLLLLIIS+Q  + SAA R  KSPA  R NTEYVRTSCS TS
Sbjct: 1   MENSHFPSIQTRPTFL-LLLLLLIISNQTQIFSAASRIPKSPAGIRKNTEYVRTSCSTTS 60

Query: 61  YPRLCYNSLSVYAGKIKTNPKTLSLAALHVNLASARSTAVAMRRLAKTRGLRRRDASAIS 120
           YPRLCYNSLSVYA KIKTNPKTL+LAALHVNLA+ARS+A +MRRLAKT GLRRR+ASAIS
Sbjct: 61  YPRLCYNSLSVYASKIKTNPKTLALAALHVNLAAARSSAASMRRLAKTHGLRRREASAIS 120

Query: 121 DCIEEVGDSVFELHRAIRELCRPRGYDFGALISDIETWVSSALTDEETCMEGFGGRSVSN 180
           DC+EEVGDSVFEL RAIREL RPRGYDFG+LISDIETWVSSALTDEETCMEGFGGR V+ 
Sbjct: 121 DCVEEVGDSVFELQRAIRELGRPRGYDFGSLISDIETWVSSALTDEETCMEGFGGRRVNG 180

Query: 181 RFSVKAKVRRHIVRVAHLTSNSLALINTYASSAAAAQGVLP 217
             SVKAKVRRHIVRV HLTSNSLALIN+YASSAAA +GV P
Sbjct: 181 VNSVKAKVRRHIVRVTHLTSNSLALINSYASSAAAEEGVFP 220

[Close]

BLAST of Cp4.1LG13g02110 vs. ExPASy TrEMBL
Match: A0A6J1E5T4 (21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430226 PE=4 SV=1)

HSP 1 Score: 389 bits (1000), Expect = 4.74e-136
Identity = 207/221 (93.67%), Postives = 211/221 (95.48%), Query Frame = 0


Query: 1   MGNFHFSLPKFLFLLLLLLIISDQIHVCSAAG----RTHKSPARFRTNTEYVRTSCSATS 60
           M NFHFSLPKFLFLLLLL IISDQIH+CSAA     RTH+SPAR RTNTEYVRTSCSATS
Sbjct: 1   MENFHFSLPKFLFLLLLL-IISDQIHICSAAAAAAARTHRSPARIRTNTEYVRTSCSATS 60

Query: 61  YPRLCYNSLSVYAGKIKTNPKTLSLAALHVNLASARSTAVAMRRLAKTRGLRRRDASAIS 120
           YPRLCYNSLSVYAGKIKTNPKTL+LAALHVNLASARSTAVAMRRLAKTRGLRRRDASAIS
Sbjct: 61  YPRLCYNSLSVYAGKIKTNPKTLALAALHVNLASARSTAVAMRRLAKTRGLRRRDASAIS 120

Query: 121 DCIEEVGDSVFELHRAIRELCRPRGYDFGALISDIETWVSSALTDEETCMEGFGGRSVSN 180
           DCIEEVGDSVFEL RAIRELCRPR YDFGALISDIETWVSSALTDEETCMEGFGGRSV+N
Sbjct: 121 DCIEEVGDSVFELQRAIRELCRPRVYDFGALISDIETWVSSALTDEETCMEGFGGRSVNN 180

Query: 181 RFSVKAKVRRHIVRVAHLTSNSLALINTYASSAAAAQGVLP 217
           RFSVKAKVRRHIVRVAHLTSNSLALINTYASSAAAAQGVLP
Sbjct: 181 RFSVKAKVRRHIVRVAHLTSNSLALINTYASSAAAAQGVLP 220

[Close]

BLAST of Cp4.1LG13g02110 vs. ExPASy TrEMBL
Match: A0A6J1I3X9 (21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111468944 PE=4 SV=1)

HSP 1 Score: 385 bits (989), Expect = 2.02e-134
Identity = 206/219 (94.06%), Postives = 208/219 (94.98%), Query Frame = 0


Query: 1   MGNFHFSLPKFLFLLLLLLIISDQIHVCSAAG--RTHKSPARFRTNTEYVRTSCSATSYP 60
           M NFHFSLPKFLFLLLL  IISDQIH+CSAA   RTHKSPAR RTNTEYVRTSCSATSYP
Sbjct: 1   MENFHFSLPKFLFLLLL--IISDQIHICSAAAATRTHKSPARIRTNTEYVRTSCSATSYP 60

Query: 61  RLCYNSLSVYAGKIKTNPKTLSLAALHVNLASARSTAVAMRRLAKTRGLRRRDASAISDC 120
           RLCYNSLSVYAGKIKTNPKTL LAALHVNLASARSTAVAMRRLAKTRGLRRRDASAISDC
Sbjct: 61  RLCYNSLSVYAGKIKTNPKTLVLAALHVNLASARSTAVAMRRLAKTRGLRRRDASAISDC 120

Query: 121 IEEVGDSVFELHRAIRELCRPRGYDFGALISDIETWVSSALTDEETCMEGFGGRSVSNRF 180
           IEEVGDSVFEL RAIREL RPRGYDFGALISDIETWVSSALTDEETCMEGFGGRSV+NRF
Sbjct: 121 IEEVGDSVFELQRAIRELRRPRGYDFGALISDIETWVSSALTDEETCMEGFGGRSVNNRF 180

Query: 181 SVKAKVRRHIVRVAHLTSNSLALINTYASSAAAAQGVLP 217
           SVKA VRRHIVRVAHLTSNSLALINTYASSAAAAQGVLP
Sbjct: 181 SVKAMVRRHIVRVAHLTSNSLALINTYASSAAAAQGVLP 217

[Close]

BLAST of Cp4.1LG13g02110 vs. ExPASy TrEMBL
Match: A0A5A7TEM1 (21 kDa protein isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00100 PE=4 SV=1)

HSP 1 Score: 322 bits (826), Expect = 1.32e-109
Identity = 176/205 (85.85%), Postives = 184/205 (89.76%), Query Frame = 0


Query: 14  LLLLLLIISDQIHVCSAAGRT-HKSPARFRTNTEYVRTSCSATSYPRLCYNSLSVYAGKI 73
           LLLLLLIISDQ  + S A  T  KS AR RTNTEYVRTSCS TSYPRLCYNSLSVYAGKI
Sbjct: 13  LLLLLLIISDQTQIFSVAASTLPKSSARIRTNTEYVRTSCSTTSYPRLCYNSLSVYAGKI 72

Query: 74  KTNPKTLSLAALHVNLASARSTAVAMRRLAKTRGLRRRDASAISDCIEEVGDSVFELHRA 133
           KTNPKTL+LAALHVNLA+ARS+A +MRRLAKTRGLRRRDASAISDC+EEVGDSVFEL RA
Sbjct: 73  KTNPKTLALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEVGDSVFELQRA 132

Query: 134 IRELCRPRGYDFGALISDIETWVSSALTDEETCMEGFGGRSVSNRFSVKAKVRRHIVRVA 193
           IREL RPRGYDF  LISDIETWVSSALTDEETCMEGFGGR V N  SVKAKVRRHIVRVA
Sbjct: 133 IRELGRPRGYDFMGLISDIETWVSSALTDEETCMEGFGGRRV-NGVSVKAKVRRHIVRVA 192

Query: 194 HLTSNSLALINTYASSAAAAQGVLP 217
           HLTSNSLALIN+YASSAAA +GVLP
Sbjct: 193 HLTSNSLALINSYASSAAAEEGVLP 216

[Close]

BLAST of Cp4.1LG13g02110 vs. ExPASy TrEMBL
Match: A0A6J1DS46 (pectinesterase inhibitor 10-like OS=Momordica charantia OX=3673 GN=LOC111023355 PE=4 SV=1)

HSP 1 Score: 271 bits (692), Expect = 2.09e-89
Identity = 151/212 (71.23%), Postives = 168/212 (79.25%), Query Frame = 0


Query: 1   MGNFHFSLPKFLFLLLLLLIISDQIHVCSAAGRTHKSPARFRTNTEYVRTSCSATSYPRL 60
           M  FHF   +F  L+  LL++        AAG         RTNTEY+RTSCSATSYPRL
Sbjct: 1   MKRFHFPSIQFSTLVSFLLLVG-------AAG--------IRTNTEYLRTSCSATSYPRL 60

Query: 61  CYNSLSVYAGKIKTNPKTLSLAALHVNLASARSTAVAMRRLAKTRGLRRRDASAISDCIE 120
           CY SLSVYAGKIKTNP+TL+LAALHVNLA+ARS+A +MRRLA+TRGLRRRDA AI DC+E
Sbjct: 61  CYTSLSVYAGKIKTNPRTLALAALHVNLAAARSSAASMRRLARTRGLRRRDALAIGDCVE 120

Query: 121 EVGDSVFELHRAIRELCRPRGYDFGALISDIETWVSSALTDEETCMEGFGGRSVSN--RF 180
           EVGDSVFEL R+I EL R R  DFGALISDIETWVSSALTDEETCMEGFGG    N  R+
Sbjct: 121 EVGDSVFELQRSIEELGRSRERDFGALISDIETWVSSALTDEETCMEGFGGGRAINGSRY 180

Query: 181 SVKAKVRRHIVRVAHLTSNSLALINTYASSAA 210
           +VKAKVRRHIV+VAH TSNSLALIN YASS+A
Sbjct: 181 NVKAKVRRHIVKVAHFTSNSLALINAYASSSA 197

[Close]

BLAST of Cp4.1LG13g02110 vs. ExPASy TrEMBL
Match: A0A1S3AUN6 (21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103483061 PE=4 SV=1)

HSP 1 Score: 263 bits (673), Expect = 9.12e-87
Identity = 141/168 (83.93%), Postives = 147/168 (87.50%), Query Frame = 0


Query: 5   HFSLPKFLFLLLLLLIISDQIHVCSAAGRT-HKSPARFRTNTEYVRTSCSATSYPRLCYN 64
           H S P    LLLLLLIISDQ  + S A  T  KSPAR RTNTEYVRTSCS TSYPRLCYN
Sbjct: 19  HSSSPS---LLLLLLIISDQTQIFSVAASTLPKSPARIRTNTEYVRTSCSTTSYPRLCYN 78

Query: 65  SLSVYAGKIKTNPKTLSLAALHVNLASARSTAVAMRRLAKTRGLRRRDASAISDCIEEVG 124
           SLSVYAGKIKTNPKTL+LAALHVNLA+ARS+A +MRRLAKTRGLRRRDASAISDC+EEVG
Sbjct: 79  SLSVYAGKIKTNPKTLALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEVG 138

Query: 125 DSVFELHRAIRELCRPRGYDFGALISDIETWVSSALTDEETCMEGFGG 171
           DSVFEL RAIREL RPRGYDF  LISDIETWVSSALTDEETCMEGFGG
Sbjct: 139 DSVFELQRAIRELGRPRGYDFMGLISDIETWVSSALTDEETCMEGFGG 183

[Close]

BLAST of Cp4.1LG13g02110 vs. TAIR 10
Match: AT5G62360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 162.2 bits (409), Expect = 4.8e-40
Identity = 90/205 (43.90%), Postives = 137/205 (66.83%), Query Frame = 0


Query: 3   NFHFSLPKFLFLLLLLLIISDQIHVCSAAGRTHKSPARFRTNTEYVRTSCSATSYPRLCY 62
           N H  L  FL ++ +L ++            T  +     TNTE+V++SC+ T+YPRLC+
Sbjct: 9   NHHHFLTTFLIIIAMLKLVH----------TTTTTTTTTTTNTEFVKSSCTFTTYPRLCF 68

Query: 63  NSLSVYAGKIKTNPKTLSLAALHVNLASARSTAVAMRRLAKTRGLRRRDASAISDCIEEV 122
           +SLS +A  I+T+PK ++ AAL++ LASA+ T+  M RL+ +R L+ ++ SA+ DC+EE+
Sbjct: 69  SSLSTHASLIQTSPKLMAHAALNITLASAKVTSAMMVRLSNSR-LKPKEVSAMRDCVEEL 128

Query: 123 GDSVFELHRAIRELCRPRGYDFGALISDIETWVSSALTDEETCMEGFGGRSVSNRFSVKA 182
           GD++ EL ++I E+C+  G ++   ISDI+TWVS+ALTD  TC +GF G  +  +  VK 
Sbjct: 129 GDTLEELRKSIGEMCQLSGSNYEVYISDIQTWVSAALTDVNTCTDGFEGEDMDGK--VKV 188

Query: 183 KVRRHIVRVAHLTSNSLALINTYAS 208
            VR  I+ +AHLTSN+LALIN +AS
Sbjct: 189 LVRGRILVIAHLTSNALALINHFAS 200

[Close]

BLAST of Cp4.1LG13g02110 vs. TAIR 10
Match: AT1G62760.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 152.5 bits (384), Expect = 3.8e-37
Identity = 81/170 (47.65%), Postives = 120/170 (70.59%), Query Frame = 0


Query: 42  RTNTEYVRTSCSATSYPRLCYNSLSVYAGKIKTNPKTLSLAALHVNLASARSTAVAMRRL 101
           +TN +Y++TSC+ T Y  +CYNSLS YA  I++NP+ L++ AL++ L+SA+S +  ++ +
Sbjct: 142 QTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKFVKNI 201

Query: 102 AKTRGLRRRDASAISDCIEEVGDSVFELHRAIRELCRPRGYD---FGALISDIETWVSSA 161
           +   GL R +  A++DC+EE+GDSV  L  +IREL      D   F  ++SD+ETWVS+A
Sbjct: 202 SHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETWVSAA 261

Query: 162 LTDEETCMEGFGGRSVSNRFSVKAKVRRHIVRVAHLTSNSLALINTYASS 209
           LT+++TCM+GF       + +VK  VRRH+V VA LTSN+LALIN YAS+
Sbjct: 262 LTNDDTCMDGFS----LVKTAVKDLVRRHVVEVARLTSNALALINMYAST 307

[Close]

BLAST of Cp4.1LG13g02110 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 140.2 bits (352), Expect = 2.0e-33
Identity = 72/168 (42.86%), Postives = 110/168 (65.48%), Query Frame = 0


Query: 44  NTEYVRTSCSATSYPRLCYNSLSVYAGKIKTNPKTLSLAALHVNLASARSTAVAMRRLAK 103
           +TE+++ SC  TSYP  C+ SLS YA +IK  P+ L+  AL V++A A+S    +  +  
Sbjct: 34  DTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALAVSIARAKSAKTYVSEMTD 93

Query: 104 TRGLRRRDASAISDCIEEVGDSVFELHRAIRELCR----PRGYDFGALISDIETWVSSAL 163
            +G+ +R   A++DC+EE+GD+V  L  +++EL        G DF   +S++ TW S+AL
Sbjct: 94  YKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGDSGEDFWFCLSNVRTWTSAAL 153

Query: 164 TDEETCMEGFGGRSVSNRFSVKAKVRRHIVRVAHLTSNSLALINTYAS 208
           TDE  CM+GFGG++++    +K+ +R HIV VA  TSN+LALIN +AS
Sbjct: 154 TDETACMDGFGGKAMAG--ELKSLIRTHIVSVAEETSNALALINDFAS 199

[Close]

BLAST of Cp4.1LG13g02110 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 139.4 bits (350), Expect = 3.4e-33
Identity = 82/204 (40.20%), Postives = 132/204 (64.71%), Query Frame = 0


Query: 10  KFLFLLL-LLLIISDQIHVCSAAGRTHKSPARFRTNTEYVRTSCSATSYPRLCYNSLSVY 69
           ++LF+LL +  ++S ++   +AA +T  S    +    ++++SC  T+YP LC +SLSVY
Sbjct: 4   QYLFVLLSISYLLSLELTAATAASQTGAS----KKAINFIQSSCKTTTYPALCVHSLSVY 63

Query: 70  AGKIKTNPKTLSLAALHVNLASARSTAVAMRRLAKTRGLRRRDASAISDCIEEVGDSVFE 129
           A  I+T+PK L+  A+ V L+ A+ST + + RL + +GL++R+  AI DC+EE+ D+V  
Sbjct: 64  ANDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDR 123

Query: 130 LHRAIRE--LC-RPRGYD-FGALISDIETWVSSALTDEETCMEGFGGRSVSNRFSVKAKV 189
           L ++++E  LC   +  D F   +S+ +TW S+ALTDE TC +GF GR +  R  +K  V
Sbjct: 124 LTKSVQELKLCGSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGR--IKNSV 183

Query: 190 RRHIVRVAHLTSNSLALINTYASS 209
           R  I+ V H TSN+L+LIN +A +
Sbjct: 184 RARIMNVGHETSNALSLINAFAKT 201

[Close]

BLAST of Cp4.1LG13g02110 vs. TAIR 10
Match: AT3G47380.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 124.8 bits (312), Expect = 8.5e-29
Identity = 77/203 (37.93%), Postives = 121/203 (59.61%), Query Frame = 0


Query: 8   LPKFLFLLLLLLIISDQIHVCSAAGRTHKSPARFRTNTEYVRTSCSATSYPRLCYNSLSV 67
           + K +F  L L ++S  I   +A+    ++    +    +++ SC AT+YP +C NSL+ 
Sbjct: 1   MAKQIFYTLFLFLLSTAI--LTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTG 60

Query: 68  YAGKIKTNPKTLSLAALHVNLASARSTAVAMRRLAKTRGLRRRDASAISDCIEEVGDSVF 127
           YA  I+T+P+ L+  AL+V +  A+ST V + RL +   L++R+  A+ DCIEE+ D+V 
Sbjct: 61  YANSIQTSPRRLAETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVD 120

Query: 128 ELHRAIRE--LC-RPRGYD-FGALISDIETWVSSALTDEETCMEGFGGRSVSNRFSVKAK 187
            L  +I E  +C   +G D F   +S+ +TW S+ALT+  TC +GF GR +  R  VK  
Sbjct: 121 RLTMSIHEVKMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGR--VKNS 180

Query: 188 VRRHIVRVAHLTSNSLALINTYA 207
           VR  I+ +   TSN+LALIN +A
Sbjct: 181 VRARILNLGRGTSNALALINAFA 199

The following BLAST results are available for this feature:

BLAST of Cp4.1LG13g02110 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SI74	5.4e-36	47.65	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]
P17407	1.2e-32	43.64	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SB37	2.8e-32	42.86	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
Q9STY5	1.2e-27	37.93	Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1	[more]
Q9SI72	1.3e-26	38.54	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]

BLAST of Cp4.1LG13g02110 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023550543.1	1.61e-147	100.00	21 kDa protein-like [Cucurbita pepo subsp. pepo]	[more]
KAG6579432.1	7.67e-136	92.83	Pectinesterase inhibitor 10, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022922163.1	9.78e-136	93.67	21 kDa protein-like [Cucurbita moschata]	[more]
XP_022969879.1	4.17e-134	94.06	21 kDa protein-like [Cucurbita maxima]	[more]
XP_038906530.1	3.84e-110	80.09	21 kDa protein-like [Benincasa hispida]	[more]

BLAST of Cp4.1LG13g02110 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E5T4	4.74e-136	93.67	21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430226 PE=4 SV=1	[more]
A0A6J1I3X9	2.02e-134	94.06	21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111468944 PE=4 SV=1	[more]
A0A5A7TEM1	1.32e-109	85.85	21 kDa protein isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]
A0A6J1DS46	2.09e-89	71.23	pectinesterase inhibitor 10-like OS=Momordica charantia OX=3673 GN=LOC111023355 ...	[more]
A0A1S3AUN6	9.12e-87	83.93	21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103483061 PE=4 SV=1	[more]

BLAST of Cp4.1LG13g02110 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62360.1	4.8e-40	43.90	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62760.1	3.8e-37	47.65	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	2.0e-33	42.86	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	3.4e-33	40.20	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G47380.1	8.5e-29	37.93	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 43..201 e-value: 1.4E-43 score: 160.8
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 47..201 e-value: 1.4E-30 score: 106.6
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 29..205 e-value: 5.4E-33 score: 111.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 43..205 e-value: 2.5E-34 score: 120.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 46..205
None	No IPR available	PANTHER	PTHR31080:SF118	PECTINESTERASE INHIBITOR 10	coord: 10..208
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 10..208
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 50..207 e-value: 5.02443E-45 score: 145.274

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG13g02110.1	Cp4.1LG13g02110.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity