Cp4.1LG13g01910 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG13g01910 vs. ExPASy Swiss-Prot
Match: Q9LNA5 (ACT domain-containing protein ACR8 OS=Arabidopsis thaliana OX=3702 GN=ACR8 PE=2 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 4.9e-161
Identity = 294/444 (66.22%), Postives = 357/444 (80.41%), Query Frame = 0

Query: 4   MEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLNLSI 63
           M   G LDEY KL+ RMN PRVVIDN VC +AT+VKVDS+RR+GILLEAVQ+LTDLNLSI
Sbjct: 1   MAMKGYLDEYEKLVIRMNTPRVVIDNGVCSSATIVKVDSSRRNGILLEAVQILTDLNLSI 60

Query: 64  QKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALELTG 123
           +KAYISSDG W MDVFHVTD+ GNKL DQ V+ Y+E+S+ T++ G+    N  TALELTG
Sbjct: 61  KKAYISSDGTWNMDVFHVTDLNGNKLNDQSVLRYIEQSIETVYYGENIEVNGLTALELTG 120

Query: 124 TDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSQKIDTIVGR 183
           TDR+GLLSE+FAVL++L CDVV+AK+WTHNGR+AS+IY+KDC S +PI DS +I  I GR
Sbjct: 121 TDRIGLLSEMFAVLSDLNCDVVDAKLWTHNGRVASVIYLKDCISGAPILDSHRISKIEGR 180

Query: 184 LRNVLKGDDDI-LYAKTSVSM-AVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTVQ 243
           L+NVL GD+D+   AKT V++ ++ H ERRLHQ+MF DRDYER+   +H      VVTVQ
Sbjct: 181 LKNVLNGDNDVNSAAKTCVTVDSMMHIERRLHQLMFEDRDYERRS-KKHERSPMVVVTVQ 240

Query: 244 NWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGTP 303
           NW +RGYSVVNV C+DR KLLFDVVCTLTDM+Y VFH TI TA D A+LEFYIRH DG+P
Sbjct: 241 NWAERGYSVVNVHCRDRTKLLFDVVCTLTDMEYAVFHATINTAEDQAHLEFYIRHKDGSP 300

Query: 304 ISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVST 363
           ISSEAERQRVIQCL+A+VERR  +GVRLEL   D+  LLA+VTRTFRENGLNVTR E+ST
Sbjct: 301 ISSEAERQRVIQCLEAAVERRALEGVRLELRHPDKQGLLAEVTRTFRENGLNVTRTEIST 360

Query: 364 SAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQKTEGEEQAAGVGG 423
           S+++A N+FYVT+A+G   D K IESVREKIGL  L+VKE+P +  +K +GEEQ      
Sbjct: 361 SSDMATNIFYVTDANGDEPDFKLIESVREKIGLECLRVKEMPTMYHKKGDGEEQQQ--TK 420

Query: 424 AVLLSLGSILRRNLYNLGLIRSYS 446
           AVL+SLGS++ RNL+N GLI+S S
Sbjct: 421 AVLVSLGSLVWRNLFNFGLIKSCS 441

BLAST of Cp4.1LG13g01910 vs. ExPASy Swiss-Prot
Match: Q8LJW1 (ACT domain-containing protein ACR7 OS=Arabidopsis thaliana OX=3702 GN=ACR7 PE=2 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 3.0e-150
Identity = 280/452 (61.95%), Postives = 345/452 (76.33%), Query Frame = 0

Query: 4   MEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLNLSI 63
           ME     +EY KL+ RMN PRVVIDN VC  +T+VK+DSAR  GILLE+VQ+LTD+NL I
Sbjct: 1   MELSDCSNEYEKLVVRMNMPRVVIDNGVCPNSTVVKIDSARSPGILLESVQLLTDMNLWI 60

Query: 64  QKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALELTG 123
           +KAYISSDG+W MDVFHV+D+ G+KLTD+ +I Y+EKS+ T H  K       TALELTG
Sbjct: 61  KKAYISSDGKWNMDVFHVSDLNGDKLTDENLIRYIEKSIETSHYCKTEGYTGLTALELTG 120

Query: 124 TDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPIN-DSQKIDTIVG 183
           TDRVGLLSE+FAVLA+L+CDVVEAK WTHNGRIAS+IYVKD NS +PI+ DS ++  + G
Sbjct: 121 TDRVGLLSEVFAVLADLECDVVEAKAWTHNGRIASMIYVKDGNSGTPIDGDSDRVQRVEG 180

Query: 184 RLRNVLKGDDDILY-AKTSVSM-AVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTV 243
           +LRN+LK DD      +T VS    TH ERRLHQ MF DRDYE+K       + SP+V+V
Sbjct: 181 QLRNLLKADDGYQNDTRTCVSYGGNTHMERRLHQRMFMDRDYEKK----FDIEKSPIVSV 240

Query: 244 QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT 303
           QN  KRGYSVVN+QCKDRMKLLFDVVCTLTDM Y+VFH  I T  +TA+LEFY+RH+DG 
Sbjct: 241 QNLPKRGYSVVNLQCKDRMKLLFDVVCTLTDMAYIVFHAAIRTVGETAFLEFYVRHSDGH 300

Query: 304 PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS 363
           P+SSE ERQR+IQCLQA++ERRT +GVRLELCT DRP LLA+VTR  RENGLN+ RAE+S
Sbjct: 301 PVSSEPERQRLIQCLQAAIERRTVKGVRLELCTADRPGLLAEVTRILRENGLNIARAEIS 360

Query: 364 TSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKE-LPLISQQKTEGEE----- 423
           T   +A N+FYVT+A+G+  D + I+S+REKIG+  L VKE  P+  ++  E E+     
Sbjct: 361 TKDSIARNVFYVTDANGNLIDPEIIKSIREKIGIDDLSVKEPFPISCREAVEKEQHIEPQ 420

Query: 424 --QAAGVGGAVLLSLGSILRRNLYNLGLIRSY 445
             Q    GG VL+SLGS++ RNLY+LGLI+SY
Sbjct: 421 DHQGRYGGGTVLVSLGSLVMRNLYHLGLIKSY 448

BLAST of Cp4.1LG13g01910 vs. ExPASy Swiss-Prot
Match: Q8LJW3 (ACT domain-containing protein ACR4 OS=Arabidopsis thaliana OX=3702 GN=ACR4 PE=2 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 5.2e-126
Identity = 243/450 (54.00%), Postives = 309/450 (68.67%), Query Frame = 0

Query: 11  DEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLNLSIQKAYISS 70
           +EY KLI RMN PRVVIDN  C+ AT+++VDSA  +GILLE VQ+LTDLNL+I KAYISS
Sbjct: 9   NEYEKLIRRMNPPRVVIDNDSCKKATVIRVDSANEYGILLEVVQILTDLNLTITKAYISS 68

Query: 71  DGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAA-------ASNDFTALELTG 130
           DG WFMDVF+VTD +GNK+TD+ V+ Y++KSL    C   +        S D T +ELTG
Sbjct: 69  DGGWFMDVFNVTDQDGNKVTDEVVLDYIQKSLGPEACFSTSMRSVGVIPSTDSTVIELTG 128

Query: 131 TDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSQKIDTIVGR 190
            DR GLLSE+ AVL  L+C V+ A++WTHN R A+++ V D  +   I+D +++  I   
Sbjct: 129 CDRPGLLSELSAVLTHLKCSVLNAEIWTHNTRAAAVMQVTDDLTGCGISDPERLSRIKNL 188

Query: 191 LRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDDDS-------- 250
           LRNVLKG +    AKT VS    HT+RRLHQMMF DRDYE + V    DDDS        
Sbjct: 189 LRNVLKGSNTPREAKTVVSHGEVHTDRRLHQMMFEDRDYEHRLV----DDDSSIQDERQR 248

Query: 251 PVVTVQNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIR 310
           P V V NW+ + YSVV V+CKDR KLLFD VCTLTDM YVVFHG++ T    A+ E+Y+R
Sbjct: 249 PDVCVDNWLDKDYSVVTVRCKDRPKLLFDTVCTLTDMQYVVFHGSVDTEGTEAFQEYYVR 308

Query: 311 HTDGTPISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVT 370
           H DG+P+ SEAE+QRVIQCL+A+++RR S+G++LELCTTDR  LL++VTR FREN L VT
Sbjct: 309 HIDGSPVKSEAEKQRVIQCLEAAIKRRVSEGLKLELCTTDRVGLLSNVTRIFRENSLTVT 368

Query: 371 RAEVSTSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQKTEGEEQ 430
           RAEV T    ALN FYV++A G + DAKTI+S+R+ IG + LKVK  P   QQ+ +    
Sbjct: 369 RAEVKTKGGKALNTFYVSDASGYSIDAKTIDSIRQTIGQTILKVKNNPQEQQQRQKSPSH 428

Query: 431 AAGVGGAVLLSLGSILRRNLYNLGLIRSYS 446
            +       L  G    ++  N GL+RSYS
Sbjct: 429 ESPTR---FLFGGLFKSKSFVNFGLVRSYS 451

BLAST of Cp4.1LG13g01910 vs. ExPASy Swiss-Prot
Match: Q9ZPQ8 (ACT domain-containing protein ACR5 OS=Arabidopsis thaliana OX=3702 GN=ACR5 PE=2 SV=2)

HSP 1 Score: 404.1 bits (1037), Expect = 2.1e-111
Identity = 231/454 (50.88%), Postives = 297/454 (65.42%), Query Frame = 0

Query: 11  DEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLNLSIQKAYISS 70
           DE +K I R+N PRVVIDN VC+  T++KVDSA +HGILLE VQVLT+LNL+I+KAYISS
Sbjct: 13  DEIAKFIRRVNPPRVVIDNEVCKDVTVIKVDSANKHGILLEVVQVLTELNLTIKKAYISS 72

Query: 71  DGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCG----------KAAASNDFTALE 130
           DG WFMDVF+VTD +GNK+TD+ V+ Y+ KSL                    S D+T +E
Sbjct: 73  DGGWFMDVFNVTDQDGNKVTDEIVLEYIRKSLGPDESSCFSPSMRSTIGVKQSVDYTVVE 132

Query: 131 LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSQKIDTI 190
           LTGTDR GLLSE+ AVL +LQC+VV A++WTH  + A+++ V D  + S I D +++  I
Sbjct: 133 LTGTDRPGLLSELCAVLMDLQCNVVNAEIWTHRAKAAAVLQVTDEETCSAITDPERLSKI 192

Query: 191 VGRLRNVLKGDDD---ILYAKTSVSMAV--THTERRLHQMMFADRDYERKPVLQHSDDDS 250
              L  VL G          KT+VS A+  THT+R+LHQ+MFADRDY+      + DD+ 
Sbjct: 193 RKLLGYVLTGGSSGRRFREPKTTVSSALNETHTDRKLHQLMFADRDYDEWE--NNVDDED 252

Query: 251 ------PVVTVQNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAY 310
                 P V V N     YS+V ++CKDR KLLFD V TLTDM+YVV H +I      AY
Sbjct: 253 KCGRVIPDVDVSNLHDLDYSIVMIKCKDRPKLLFDTVFTLTDMNYVVSHASIDAEGPQAY 312

Query: 311 LEFYIRHTDGTPISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRE 370
            E+YIRHTDG+P+ SEAERQRVI+CL+A+++RR S+G++LELCT+DR  LL+DVTR FRE
Sbjct: 313 QEYYIRHTDGSPVKSEAERQRVIKCLKAAIQRRVSEGLKLELCTSDRVGLLSDVTRIFRE 372

Query: 371 NGLNVTRAEVSTSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQK 430
           N L VTRAEV T  + ALN FYV +A G   D KTIES+R+ IG + L+VK     +  K
Sbjct: 373 NSLTVTRAEVKTKGDKALNTFYVRDASGYQVDTKTIESIRQVIGQTILQVKGGN--TDAK 432

Query: 431 TEGEEQAAGVGGAVLLSLGSILRRNLYNLGLIRS 444
              ++   G         G    R+  N GLIRS
Sbjct: 433 PSPQDSPTG------FLFGVFKSRSFVNFGLIRS 456

BLAST of Cp4.1LG13g01910 vs. ExPASy Swiss-Prot
Match: Q9SGA0 (ACT domain-containing protein ACR6 OS=Arabidopsis thaliana OX=3702 GN=ACR6 PE=1 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 4.0e-110
Identity = 211/401 (52.62%), Postives = 284/401 (70.82%), Query Frame = 0

Query: 11  DEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLNLSIQKAYISS 70
           DEY+KLI RMN PRVVIDN   + AT+++VDS  +HG LLE VQVLTD+NL I+KAYISS
Sbjct: 4   DEYAKLIRRMNPPRVVIDNNASDDATVIQVDSVNKHGTLLEVVQVLTDMNLVIKKAYISS 63

Query: 71  DGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCG----------KAAASNDFTALE 130
           DG WFMDVF V D +GNK+ D  V+ Y++K + + + G              ++++T++E
Sbjct: 64  DGGWFMDVFKVIDQDGNKIRDTQVLDYIQKRIES-NAGWFIPPLRSSVGVMPTDEYTSIE 123

Query: 131 LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSQKIDTI 190
           L GTDR GLLSE+ AVL +L C+VV A++WTHN R A++I+V D ++ S I D  ++ TI
Sbjct: 124 LAGTDRPGLLSEVSAVLTDLHCNVVNAEIWTHNTRAAAVIHVTDNSTHSAITDPIRLSTI 183

Query: 191 VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTV 250
              L NV++ +     AKT  S + TH ERRLHQ+MF DRDYE     + S    P VT+
Sbjct: 184 KELLCNVVRTNSGSRAAKTVFSCSDTHRERRLHQIMFDDRDYEGVKRARTS-ASRPSVTL 243

Query: 251 QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT 310
            N +++ Y+VV ++ KDR KL+FDVVCTLTDM YVVFHG ++T    AY EFYIRH DG 
Sbjct: 244 MN-IEKDYTVVTMRSKDRPKLVFDVVCTLTDMQYVVFHGMVSTEPVEAYQEFYIRHVDGL 303

Query: 311 PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS 370
           PI+SEAE++RVIQCL+A++ERR S+G+ LEL   DR  LL+D+TRTFREN L + RAE+S
Sbjct: 304 PINSEAEQERVIQCLEAAIERRASEGLELELSAEDRVGLLSDITRTFRENSLTIVRAEIS 363

Query: 371 TSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKE 402
           T    A + FYVT+  G+  ++K +ES+R++IG+S LKVK+
Sbjct: 364 TREGKAKDTFYVTDVTGNPVESKIVESIRQQIGVSKLKVKK 401

BLAST of Cp4.1LG13g01910 vs. NCBI nr
Match: XP_023549665.1 (ACT domain-containing protein ACR8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 871 bits (2251), Expect = 0.0
Identity = 445/445 (100.00%), Postives = 445/445 (100.00%), Query Frame = 0

Query: 1   MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLN 60
           MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLN
Sbjct: 1   MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLN 60

Query: 61  LSIQKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALE 120
           LSIQKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALE
Sbjct: 61  LSIQKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALE 120

Query: 121 LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSQKIDTI 180
           LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSQKIDTI
Sbjct: 121 LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSQKIDTI 180

Query: 181 VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTV 240
           VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTV
Sbjct: 181 VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTV 240

Query: 241 QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT 300
           QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT
Sbjct: 241 QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT 300

Query: 301 PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS 360
           PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS
Sbjct: 301 PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS 360

Query: 361 TSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQKTEGEEQAAGVG 420
           TSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQKTEGEEQAAGVG
Sbjct: 361 TSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQKTEGEEQAAGVG 420

Query: 421 GAVLLSLGSILRRNLYNLGLIRSYS 445
           GAVLLSLGSILRRNLYNLGLIRSYS
Sbjct: 421 GAVLLSLGSILRRNLYNLGLIRSYS 445

BLAST of Cp4.1LG13g01910 vs. NCBI nr
Match: XP_022970122.1 (ACT domain-containing protein ACR8-like [Cucurbita maxima])

HSP 1 Score: 867 bits (2241), Expect = 0.0
Identity = 442/445 (99.33%), Postives = 444/445 (99.78%), Query Frame = 0

Query: 1   MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLN 60
           MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGIL+EAVQVLTDLN
Sbjct: 1   MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILVEAVQVLTDLN 60

Query: 61  LSIQKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALE 120
           LSIQKAYISSDGRWFMDVFHVTDIEGNKLTD+GVISYLEKSLATIHCGK AASNDFTALE
Sbjct: 61  LSIQKAYISSDGRWFMDVFHVTDIEGNKLTDEGVISYLEKSLATIHCGKTAASNDFTALE 120

Query: 121 LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSQKIDTI 180
           LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSQKIDTI
Sbjct: 121 LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSQKIDTI 180

Query: 181 VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTV 240
           VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTV
Sbjct: 181 VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTV 240

Query: 241 QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT 300
           QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT
Sbjct: 241 QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT 300

Query: 301 PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS 360
           PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS
Sbjct: 301 PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS 360

Query: 361 TSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQKTEGEEQAAGVG 420
           TSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQKTEGEEQAAGVG
Sbjct: 361 TSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQKTEGEEQAAGVG 420

Query: 421 GAVLLSLGSILRRNLYNLGLIRSYS 445
           GAVLLSLGSILRRNLYNLGLIRSYS
Sbjct: 421 GAVLLSLGSILRRNLYNLGLIRSYS 445

BLAST of Cp4.1LG13g01910 vs. NCBI nr
Match: XP_022922407.1 (ACT domain-containing protein ACR8-like [Cucurbita moschata])

HSP 1 Score: 865 bits (2234), Expect = 0.0
Identity = 440/445 (98.88%), Postives = 444/445 (99.78%), Query Frame = 0

Query: 1   MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLN 60
           MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLN
Sbjct: 1   MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLN 60

Query: 61  LSIQKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALE 120
           LSIQKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALE
Sbjct: 61  LSIQKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALE 120

Query: 121 LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSQKIDTI 180
           LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPIND+QKIDTI
Sbjct: 121 LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDAQKIDTI 180

Query: 181 VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTV 240
           VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQH DDDSPVVTV
Sbjct: 181 VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHGDDDSPVVTV 240

Query: 241 QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT 300
           QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT
Sbjct: 241 QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT 300

Query: 301 PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS 360
           PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS
Sbjct: 301 PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS 360

Query: 361 TSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQKTEGEEQAAGVG 420
           TS++VALNLFYVTNAHGSAADAKTI+SVREKIGLSSLKVKELPLISQQKTEGEEQAAGVG
Sbjct: 361 TSSDVALNLFYVTNAHGSAADAKTIKSVREKIGLSSLKVKELPLISQQKTEGEEQAAGVG 420

Query: 421 GAVLLSLGSILRRNLYNLGLIRSYS 445
           GAVLLSLGSILRRNLYNLGLIRSYS
Sbjct: 421 GAVLLSLGSILRRNLYNLGLIRSYS 445

BLAST of Cp4.1LG13g01910 vs. NCBI nr
Match: KAG6579451.1 (ACT domain-containing protein ACR8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 846 bits (2185), Expect = 5.49e-308
Identity = 434/445 (97.53%), Postives = 437/445 (98.20%), Query Frame = 0

Query: 1   MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLN 60
           MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLN
Sbjct: 53  MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLN 112

Query: 61  LSIQKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALE 120
           LSIQKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALE
Sbjct: 113 LSIQKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALE 172

Query: 121 LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSQKIDTI 180
           LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDS+KIDTI
Sbjct: 173 LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSEKIDTI 232

Query: 181 VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTV 240
           VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTV
Sbjct: 233 VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTV 292

Query: 241 QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT 300
           QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT
Sbjct: 293 QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT 352

Query: 301 PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS 360
           PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS
Sbjct: 353 PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS 412

Query: 361 TSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQKTEGEEQAAGVG 420
           TS++VALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQKTEGEE      
Sbjct: 413 TSSDVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQKTEGEEH----- 472

Query: 421 GAVLLSLGSILRRNLYNLGLIRSYS 445
             VLLSLGSILRRNLYNLGLIRSYS
Sbjct: 473 --VLLSLGSILRRNLYNLGLIRSYS 490

BLAST of Cp4.1LG13g01910 vs. NCBI nr
Match: KAG7016924.1 (ACT domain-containing protein ACR8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 833 bits (2153), Expect = 8.44e-304
Identity = 432/455 (94.95%), Postives = 435/455 (95.60%), Query Frame = 0

Query: 1   MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLN 60
           MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLN
Sbjct: 1   MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLN 60

Query: 61  LSIQKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALE 120
           LSIQKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALE
Sbjct: 61  LSIQKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALE 120

Query: 121 LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSQKIDTI 180
           LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDS+KIDTI
Sbjct: 121 LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSEKIDTI 180

Query: 181 VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTV 240
           VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTV
Sbjct: 181 VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTV 240

Query: 241 QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYL---------- 300
           QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYL          
Sbjct: 241 QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLSHQLSVTSHQ 300

Query: 301 EFYIRHTDGTPISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFREN 360
           EFYIRHTDGTPISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFREN
Sbjct: 301 EFYIRHTDGTPISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFREN 360

Query: 361 GLNVTRAEVSTSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQKT 420
           GLNVTRAEVSTS++VALNLFYVTNAHGSAAD KTIESVREKIGLSSLKVKELPLISQQKT
Sbjct: 361 GLNVTRAEVSTSSDVALNLFYVTNAHGSAADVKTIESVREKIGLSSLKVKELPLISQQKT 420

Query: 421 EGEEQAAGVGGAVLLSLGSILRRNLYNLGLIRSYS 445
            GEE        VLLSLGSILRRNLYNLGLIRSYS
Sbjct: 421 GGEEH-------VLLSLGSILRRNLYNLGLIRSYS 448

BLAST of Cp4.1LG13g01910 vs. ExPASy TrEMBL
Match: A0A6J1HY87 (ACT domain-containing protein ACR8-like OS=Cucurbita maxima OX=3661 GN=LOC111469115 PE=4 SV=1)

HSP 1 Score: 867 bits (2241), Expect = 0.0
Identity = 442/445 (99.33%), Postives = 444/445 (99.78%), Query Frame = 0

Query: 1   MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLN 60
           MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGIL+EAVQVLTDLN
Sbjct: 1   MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILVEAVQVLTDLN 60

Query: 61  LSIQKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALE 120
           LSIQKAYISSDGRWFMDVFHVTDIEGNKLTD+GVISYLEKSLATIHCGK AASNDFTALE
Sbjct: 61  LSIQKAYISSDGRWFMDVFHVTDIEGNKLTDEGVISYLEKSLATIHCGKTAASNDFTALE 120

Query: 121 LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSQKIDTI 180
           LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSQKIDTI
Sbjct: 121 LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSQKIDTI 180

Query: 181 VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTV 240
           VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTV
Sbjct: 181 VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTV 240

Query: 241 QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT 300
           QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT
Sbjct: 241 QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT 300

Query: 301 PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS 360
           PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS
Sbjct: 301 PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS 360

Query: 361 TSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQKTEGEEQAAGVG 420
           TSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQKTEGEEQAAGVG
Sbjct: 361 TSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQKTEGEEQAAGVG 420

Query: 421 GAVLLSLGSILRRNLYNLGLIRSYS 445
           GAVLLSLGSILRRNLYNLGLIRSYS
Sbjct: 421 GAVLLSLGSILRRNLYNLGLIRSYS 445

BLAST of Cp4.1LG13g01910 vs. ExPASy TrEMBL
Match: A0A6J1E360 (ACT domain-containing protein ACR8-like OS=Cucurbita moschata OX=3662 GN=LOC111430413 PE=4 SV=1)

HSP 1 Score: 865 bits (2234), Expect = 0.0
Identity = 440/445 (98.88%), Postives = 444/445 (99.78%), Query Frame = 0

Query: 1   MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLN 60
           MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLN
Sbjct: 1   MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLN 60

Query: 61  LSIQKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALE 120
           LSIQKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALE
Sbjct: 61  LSIQKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALE 120

Query: 121 LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSQKIDTI 180
           LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPIND+QKIDTI
Sbjct: 121 LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDAQKIDTI 180

Query: 181 VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTV 240
           VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQH DDDSPVVTV
Sbjct: 181 VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHGDDDSPVVTV 240

Query: 241 QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT 300
           QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT
Sbjct: 241 QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT 300

Query: 301 PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS 360
           PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS
Sbjct: 301 PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS 360

Query: 361 TSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQKTEGEEQAAGVG 420
           TS++VALNLFYVTNAHGSAADAKTI+SVREKIGLSSLKVKELPLISQQKTEGEEQAAGVG
Sbjct: 361 TSSDVALNLFYVTNAHGSAADAKTIKSVREKIGLSSLKVKELPLISQQKTEGEEQAAGVG 420

Query: 421 GAVLLSLGSILRRNLYNLGLIRSYS 445
           GAVLLSLGSILRRNLYNLGLIRSYS
Sbjct: 421 GAVLLSLGSILRRNLYNLGLIRSYS 445

BLAST of Cp4.1LG13g01910 vs. ExPASy TrEMBL
Match: A0A5A7THS3 (ACT domain-containing protein ACR8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003490 PE=4 SV=1)

HSP 1 Score: 803 bits (2074), Expect = 4.45e-292
Identity = 408/445 (91.69%), Postives = 426/445 (95.73%), Query Frame = 0

Query: 1   MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLN 60
           MV++EWPGSLDEYSKLINRMN PRVVIDN VCETATLVKVDSARRHGILLEAVQVLTDLN
Sbjct: 5   MVDIEWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 64

Query: 61  LSIQKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALE 120
           LSIQKAYISSDG WFMDVFHVTD+EGNKLTD+GVISYLE+SLATIHCGK A SND TALE
Sbjct: 65  LSIQKAYISSDGIWFMDVFHVTDLEGNKLTDEGVISYLEQSLATIHCGKPATSNDLTALE 124

Query: 121 LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSQKIDTI 180
           LTGTDRVGLLSE+FAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNS SPI DS+KIDTI
Sbjct: 125 LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSEKIDTI 184

Query: 181 VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTV 240
           VGRLRNVLKGDDDILYAKTSVSM VTHTERRLHQMMFADRDYERKPVLQHS+D SPVVTV
Sbjct: 185 VGRLRNVLKGDDDILYAKTSVSMTVTHTERRLHQMMFADRDYERKPVLQHSED-SPVVTV 244

Query: 241 QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT 300
           QN V+RGYSVVNVQCKDRMKLLFDV+CT+TDMDYVVFHGTITT+   AYLEFYIRHTDGT
Sbjct: 245 QNLVERGYSVVNVQCKDRMKLLFDVICTMTDMDYVVFHGTITTSRHRAYLEFYIRHTDGT 304

Query: 301 PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS 360
           PISSEAERQRVIQCLQAS+ERRTS+GVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS
Sbjct: 305 PISSEAERQRVIQCLQASIERRTSRGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS 364

Query: 361 TSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQKTEGEEQAAGVG 420
           TS EVALNLFYVT+ HGSAAD K I+SVREKIG+S+LKVKELPLISQQKTEGEEQAA VG
Sbjct: 365 TSQEVALNLFYVTDGHGSAADTKMIDSVREKIGVSNLKVKELPLISQQKTEGEEQAASVG 424

Query: 421 GAVLLSLGSILRRNLYNLGLIRSYS 445
           GAVLLSLGSILRRNLYNLGLIRSYS
Sbjct: 425 GAVLLSLGSILRRNLYNLGLIRSYS 448

BLAST of Cp4.1LG13g01910 vs. ExPASy TrEMBL
Match: A0A1S3ATY2 (ACT domain-containing protein ACR8-like OS=Cucumis melo OX=3656 GN=LOC103482670 PE=4 SV=1)

HSP 1 Score: 803 bits (2074), Expect = 4.45e-292
Identity = 408/445 (91.69%), Postives = 426/445 (95.73%), Query Frame = 0

Query: 1   MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLN 60
           MV++EWPGSLDEYSKLINRMN PRVVIDN VCETATLVKVDSARRHGILLEAVQVLTDLN
Sbjct: 5   MVDIEWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 64

Query: 61  LSIQKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALE 120
           LSIQKAYISSDG WFMDVFHVTD+EGNKLTD+GVISYLE+SLATIHCGK A SND TALE
Sbjct: 65  LSIQKAYISSDGIWFMDVFHVTDLEGNKLTDEGVISYLEQSLATIHCGKPATSNDLTALE 124

Query: 121 LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSQKIDTI 180
           LTGTDRVGLLSE+FAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNS SPI DS+KIDTI
Sbjct: 125 LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSEKIDTI 184

Query: 181 VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTV 240
           VGRLRNVLKGDDDILYAKTSVSM VTHTERRLHQMMFADRDYERKPVLQHS+D SPVVTV
Sbjct: 185 VGRLRNVLKGDDDILYAKTSVSMTVTHTERRLHQMMFADRDYERKPVLQHSED-SPVVTV 244

Query: 241 QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT 300
           QN V+RGYSVVNVQCKDRMKLLFDV+CT+TDMDYVVFHGTITT+   AYLEFYIRHTDGT
Sbjct: 245 QNLVERGYSVVNVQCKDRMKLLFDVICTMTDMDYVVFHGTITTSRHRAYLEFYIRHTDGT 304

Query: 301 PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS 360
           PISSEAERQRVIQCLQAS+ERRTS+GVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS
Sbjct: 305 PISSEAERQRVIQCLQASIERRTSRGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS 364

Query: 361 TSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQKTEGEEQAAGVG 420
           TS EVALNLFYVT+ HGSAAD K I+SVREKIG+S+LKVKELPLISQQKTEGEEQAA VG
Sbjct: 365 TSQEVALNLFYVTDGHGSAADTKMIDSVREKIGVSNLKVKELPLISQQKTEGEEQAASVG 424

Query: 421 GAVLLSLGSILRRNLYNLGLIRSYS 445
           GAVLLSLGSILRRNLYNLGLIRSYS
Sbjct: 425 GAVLLSLGSILRRNLYNLGLIRSYS 448

BLAST of Cp4.1LG13g01910 vs. ExPASy TrEMBL
Match: A0A6J1H146 (ACT domain-containing protein ACR8-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459196 PE=4 SV=1)

HSP 1 Score: 803 bits (2073), Expect = 5.86e-292
Identity = 412/445 (92.58%), Postives = 422/445 (94.83%), Query Frame = 0

Query: 1   MVEMEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLN 60
           MVEMEWPGSLDEYSKLINR+N PRVVIDN VCETATLVKVDSARRHGILLEAVQVLTDLN
Sbjct: 1   MVEMEWPGSLDEYSKLINRINIPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 60

Query: 61  LSIQKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALE 120
           LSIQKAYISSDG WFMDVFHVTD++GNKL D+GVISYLEKSLATIHCGK AASND TALE
Sbjct: 61  LSIQKAYISSDGIWFMDVFHVTDLDGNKLIDEGVISYLEKSLATIHCGKPAASNDLTALE 120

Query: 121 LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSQKIDTI 180
           LTGTDRVGLLSE+FAVLAEL+CDVVEAKVWTHNGRIASLIYVKDCNS SPI DSQKIDTI
Sbjct: 121 LTGTDRVGLLSEVFAVLAELECDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTI 180

Query: 181 VGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTV 240
           VGRLRNVLKG DDILYAKTSVSM VTHTERRLHQMMFADRDYERKPVLQHSDD SPVVTV
Sbjct: 181 VGRLRNVLKGQDDILYAKTSVSMTVTHTERRLHQMMFADRDYERKPVLQHSDD-SPVVTV 240

Query: 241 QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT 300
           QNWV+RGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTA D AYLE YIRHTDGT
Sbjct: 241 QNWVERGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAKDRAYLELYIRHTDGT 300

Query: 301 PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS 360
           PISSEAERQRVIQCLQASVERRTSQGVRLEL TTDRPCLLADVTRTFRENGLNVTRAEVS
Sbjct: 301 PISSEAERQRVIQCLQASVERRTSQGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVS 360

Query: 361 TSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQKTEGEEQAAGVG 420
           TSA+VALNLFYVT+A G+AAD KTI SVREKIGLS LKVKELPLISQQKTEGEEQ  GVG
Sbjct: 361 TSADVALNLFYVTDALGNAADVKTINSVREKIGLSCLKVKELPLISQQKTEGEEQGVGVG 420

Query: 421 GAVLLSLGSILRRNLYNLGLIRSYS 445
           G VL SLGSILRRNLYNLGLIRSYS
Sbjct: 421 GTVLFSLGSILRRNLYNLGLIRSYS 444

BLAST of Cp4.1LG13g01910 vs. TAIR 10
Match: AT1G12420.1 (ACT domain repeat 8 )

HSP 1 Score: 568.9 bits (1465), Expect = 3.5e-162
Identity = 294/444 (66.22%), Postives = 357/444 (80.41%), Query Frame = 0

Query: 4   MEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLNLSI 63
           M   G LDEY KL+ RMN PRVVIDN VC +AT+VKVDS+RR+GILLEAVQ+LTDLNLSI
Sbjct: 1   MAMKGYLDEYEKLVIRMNTPRVVIDNGVCSSATIVKVDSSRRNGILLEAVQILTDLNLSI 60

Query: 64  QKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALELTG 123
           +KAYISSDG W MDVFHVTD+ GNKL DQ V+ Y+E+S+ T++ G+    N  TALELTG
Sbjct: 61  KKAYISSDGTWNMDVFHVTDLNGNKLNDQSVLRYIEQSIETVYYGENIEVNGLTALELTG 120

Query: 124 TDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSQKIDTIVGR 183
           TDR+GLLSE+FAVL++L CDVV+AK+WTHNGR+AS+IY+KDC S +PI DS +I  I GR
Sbjct: 121 TDRIGLLSEMFAVLSDLNCDVVDAKLWTHNGRVASVIYLKDCISGAPILDSHRISKIEGR 180

Query: 184 LRNVLKGDDDI-LYAKTSVSM-AVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTVQ 243
           L+NVL GD+D+   AKT V++ ++ H ERRLHQ+MF DRDYER+   +H      VVTVQ
Sbjct: 181 LKNVLNGDNDVNSAAKTCVTVDSMMHIERRLHQLMFEDRDYERRS-KKHERSPMVVVTVQ 240

Query: 244 NWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGTP 303
           NW +RGYSVVNV C+DR KLLFDVVCTLTDM+Y VFH TI TA D A+LEFYIRH DG+P
Sbjct: 241 NWAERGYSVVNVHCRDRTKLLFDVVCTLTDMEYAVFHATINTAEDQAHLEFYIRHKDGSP 300

Query: 304 ISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVST 363
           ISSEAERQRVIQCL+A+VERR  +GVRLEL   D+  LLA+VTRTFRENGLNVTR E+ST
Sbjct: 301 ISSEAERQRVIQCLEAAVERRALEGVRLELRHPDKQGLLAEVTRTFRENGLNVTRTEIST 360

Query: 364 SAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQKTEGEEQAAGVGG 423
           S+++A N+FYVT+A+G   D K IESVREKIGL  L+VKE+P +  +K +GEEQ      
Sbjct: 361 SSDMATNIFYVTDANGDEPDFKLIESVREKIGLECLRVKEMPTMYHKKGDGEEQQQ--TK 420

Query: 424 AVLLSLGSILRRNLYNLGLIRSYS 446
           AVL+SLGS++ RNL+N GLI+S S
Sbjct: 421 AVLVSLGSLVWRNLFNFGLIKSCS 441

BLAST of Cp4.1LG13g01910 vs. TAIR 10
Match: AT4G22780.1 (ACT domain repeat 7 )

HSP 1 Score: 533.1 bits (1372), Expect = 2.1e-151
Identity = 280/452 (61.95%), Postives = 345/452 (76.33%), Query Frame = 0

Query: 4   MEWPGSLDEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLNLSI 63
           ME     +EY KL+ RMN PRVVIDN VC  +T+VK+DSAR  GILLE+VQ+LTD+NL I
Sbjct: 1   MELSDCSNEYEKLVVRMNMPRVVIDNGVCPNSTVVKIDSARSPGILLESVQLLTDMNLWI 60

Query: 64  QKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAAASNDFTALELTG 123
           +KAYISSDG+W MDVFHV+D+ G+KLTD+ +I Y+EKS+ T H  K       TALELTG
Sbjct: 61  KKAYISSDGKWNMDVFHVSDLNGDKLTDENLIRYIEKSIETSHYCKTEGYTGLTALELTG 120

Query: 124 TDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPIN-DSQKIDTIVG 183
           TDRVGLLSE+FAVLA+L+CDVVEAK WTHNGRIAS+IYVKD NS +PI+ DS ++  + G
Sbjct: 121 TDRVGLLSEVFAVLADLECDVVEAKAWTHNGRIASMIYVKDGNSGTPIDGDSDRVQRVEG 180

Query: 184 RLRNVLKGDDDILY-AKTSVSM-AVTHTERRLHQMMFADRDYERKPVLQHSDDDSPVVTV 243
           +LRN+LK DD      +T VS    TH ERRLHQ MF DRDYE+K       + SP+V+V
Sbjct: 181 QLRNLLKADDGYQNDTRTCVSYGGNTHMERRLHQRMFMDRDYEKK----FDIEKSPIVSV 240

Query: 244 QNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIRHTDGT 303
           QN  KRGYSVVN+QCKDRMKLLFDVVCTLTDM Y+VFH  I T  +TA+LEFY+RH+DG 
Sbjct: 241 QNLPKRGYSVVNLQCKDRMKLLFDVVCTLTDMAYIVFHAAIRTVGETAFLEFYVRHSDGH 300

Query: 304 PISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVS 363
           P+SSE ERQR+IQCLQA++ERRT +GVRLELCT DRP LLA+VTR  RENGLN+ RAE+S
Sbjct: 301 PVSSEPERQRLIQCLQAAIERRTVKGVRLELCTADRPGLLAEVTRILRENGLNIARAEIS 360

Query: 364 TSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKE-LPLISQQKTEGEE----- 423
           T   +A N+FYVT+A+G+  D + I+S+REKIG+  L VKE  P+  ++  E E+     
Sbjct: 361 TKDSIARNVFYVTDANGNLIDPEIIKSIREKIGIDDLSVKEPFPISCREAVEKEQHIEPQ 420

Query: 424 --QAAGVGGAVLLSLGSILRRNLYNLGLIRSY 445
             Q    GG VL+SLGS++ RNLY+LGLI+SY
Sbjct: 421 DHQGRYGGGTVLVSLGSLVMRNLYHLGLIKSY 448

BLAST of Cp4.1LG13g01910 vs. TAIR 10
Match: AT1G69040.2 (ACT domain repeat 4 )

HSP 1 Score: 453.8 bits (1166), Expect = 1.6e-127
Identity = 246/460 (53.48%), Postives = 313/460 (68.04%), Query Frame = 0

Query: 2   VEMEWPGSLD-EYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLN 61
           V M +   +D EY KLI RMN PRVVIDN  C+ AT+++VDSA  +GILLE VQ+LTDLN
Sbjct: 3   VSMSFSQDMDNEYEKLIRRMNPPRVVIDNDSCKKATVIRVDSANEYGILLEVVQILTDLN 62

Query: 62  LSIQKAYISSDGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAA-------AS 121
           L+I KAYISSDG WFMDVF+VTD +GNK+TD+ V+ Y++KSL    C   +        S
Sbjct: 63  LTITKAYISSDGGWFMDVFNVTDQDGNKVTDEVVLDYIQKSLGPEACFSTSMRSVGVIPS 122

Query: 122 NDFTALELTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPIND 181
            D T +ELTG DR GLLSE+ AVL  L+C V+ A++WTHN R A+++ V D  +   I+D
Sbjct: 123 TDSTVIELTGCDRPGLLSELSAVLTHLKCSVLNAEIWTHNTRAAAVMQVTDDLTGCGISD 182

Query: 182 SQKIDTIVGRLRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDD 241
            +++  I   LRNVLKG +    AKT VS    HT+RRLHQMMF DRDYE + V    DD
Sbjct: 183 PERLSRIKNLLRNVLKGSNTPREAKTVVSHGEVHTDRRLHQMMFEDRDYEHRLV----DD 242

Query: 242 DS--------PVVTVQNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAM 301
           DS        P V V NW+ + YSVV V+CKDR KLLFD VCTLTDM YVVFHG++ T  
Sbjct: 243 DSSIQDERQRPDVCVDNWLDKDYSVVTVRCKDRPKLLFDTVCTLTDMQYVVFHGSVDTEG 302

Query: 302 DTAYLEFYIRHTDGTPISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTR 361
             A+ E+Y+RH DG+P+ SEAE+QRVIQCL+A+++RR S+G++LELCTTDR  LL++VTR
Sbjct: 303 TEAFQEYYVRHIDGSPVKSEAEKQRVIQCLEAAIKRRVSEGLKLELCTTDRVGLLSNVTR 362

Query: 362 TFRENGLNVTRAEVSTSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLI 421
            FREN L VTRAEV T    ALN FYV++A G + DAKTI+S+R+ IG + LKVK  P  
Sbjct: 363 IFRENSLTVTRAEVKTKGGKALNTFYVSDASGYSIDAKTIDSIRQTIGQTILKVKNNPQE 422

Query: 422 SQQKTEGEEQAAGVGGAVLLSLGSILRRNLYNLGLIRSYS 446
            QQ+ +     +       L  G    ++  N GL+RSYS
Sbjct: 423 QQQRQKSPSHESPTR---FLFGGLFKSKSFVNFGLVRSYS 455

BLAST of Cp4.1LG13g01910 vs. TAIR 10
Match: AT1G69040.1 (ACT domain repeat 4 )

HSP 1 Score: 452.6 bits (1163), Expect = 3.7e-127
Identity = 243/450 (54.00%), Postives = 309/450 (68.67%), Query Frame = 0

Query: 11  DEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLNLSIQKAYISS 70
           +EY KLI RMN PRVVIDN  C+ AT+++VDSA  +GILLE VQ+LTDLNL+I KAYISS
Sbjct: 9   NEYEKLIRRMNPPRVVIDNDSCKKATVIRVDSANEYGILLEVVQILTDLNLTITKAYISS 68

Query: 71  DGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCGKAA-------ASNDFTALELTG 130
           DG WFMDVF+VTD +GNK+TD+ V+ Y++KSL    C   +        S D T +ELTG
Sbjct: 69  DGGWFMDVFNVTDQDGNKVTDEVVLDYIQKSLGPEACFSTSMRSVGVIPSTDSTVIELTG 128

Query: 131 TDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSQKIDTIVGR 190
            DR GLLSE+ AVL  L+C V+ A++WTHN R A+++ V D  +   I+D +++  I   
Sbjct: 129 CDRPGLLSELSAVLTHLKCSVLNAEIWTHNTRAAAVMQVTDDLTGCGISDPERLSRIKNL 188

Query: 191 LRNVLKGDDDILYAKTSVSMAVTHTERRLHQMMFADRDYERKPVLQHSDDDS-------- 250
           LRNVLKG +    AKT VS    HT+RRLHQMMF DRDYE + V    DDDS        
Sbjct: 189 LRNVLKGSNTPREAKTVVSHGEVHTDRRLHQMMFEDRDYEHRLV----DDDSSIQDERQR 248

Query: 251 PVVTVQNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAYLEFYIR 310
           P V V NW+ + YSVV V+CKDR KLLFD VCTLTDM YVVFHG++ T    A+ E+Y+R
Sbjct: 249 PDVCVDNWLDKDYSVVTVRCKDRPKLLFDTVCTLTDMQYVVFHGSVDTEGTEAFQEYYVR 308

Query: 311 HTDGTPISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRENGLNVT 370
           H DG+P+ SEAE+QRVIQCL+A+++RR S+G++LELCTTDR  LL++VTR FREN L VT
Sbjct: 309 HIDGSPVKSEAEKQRVIQCLEAAIKRRVSEGLKLELCTTDRVGLLSNVTRIFRENSLTVT 368

Query: 371 RAEVSTSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQKTEGEEQ 430
           RAEV T    ALN FYV++A G + DAKTI+S+R+ IG + LKVK  P   QQ+ +    
Sbjct: 369 RAEVKTKGGKALNTFYVSDASGYSIDAKTIDSIRQTIGQTILKVKNNPQEQQQRQKSPSH 428

Query: 431 AAGVGGAVLLSLGSILRRNLYNLGLIRSYS 446
            +       L  G    ++  N GL+RSYS
Sbjct: 429 ESPTR---FLFGGLFKSKSFVNFGLVRSYS 451

BLAST of Cp4.1LG13g01910 vs. TAIR 10
Match: AT2G03730.1 (ACT domain repeat 5 )

HSP 1 Score: 404.1 bits (1037), Expect = 1.5e-112
Identity = 231/454 (50.88%), Postives = 297/454 (65.42%), Query Frame = 0

Query: 11  DEYSKLINRMNAPRVVIDNTVCETATLVKVDSARRHGILLEAVQVLTDLNLSIQKAYISS 70
           DE +K I R+N PRVVIDN VC+  T++KVDSA +HGILLE VQVLT+LNL+I+KAYISS
Sbjct: 13  DEIAKFIRRVNPPRVVIDNEVCKDVTVIKVDSANKHGILLEVVQVLTELNLTIKKAYISS 72

Query: 71  DGRWFMDVFHVTDIEGNKLTDQGVISYLEKSLATIHCG----------KAAASNDFTALE 130
           DG WFMDVF+VTD +GNK+TD+ V+ Y+ KSL                    S D+T +E
Sbjct: 73  DGGWFMDVFNVTDQDGNKVTDEIVLEYIRKSLGPDESSCFSPSMRSTIGVKQSVDYTVVE 132

Query: 131 LTGTDRVGLLSEIFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSDSPINDSQKIDTI 190
           LTGTDR GLLSE+ AVL +LQC+VV A++WTH  + A+++ V D  + S I D +++  I
Sbjct: 133 LTGTDRPGLLSELCAVLMDLQCNVVNAEIWTHRAKAAAVLQVTDEETCSAITDPERLSKI 192

Query: 191 VGRLRNVLKGDDD---ILYAKTSVSMAV--THTERRLHQMMFADRDYERKPVLQHSDDDS 250
              L  VL G          KT+VS A+  THT+R+LHQ+MFADRDY+      + DD+ 
Sbjct: 193 RKLLGYVLTGGSSGRRFREPKTTVSSALNETHTDRKLHQLMFADRDYDEWE--NNVDDED 252

Query: 251 ------PVVTVQNWVKRGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAMDTAY 310
                 P V V N     YS+V ++CKDR KLLFD V TLTDM+YVV H +I      AY
Sbjct: 253 KCGRVIPDVDVSNLHDLDYSIVMIKCKDRPKLLFDTVFTLTDMNYVVSHASIDAEGPQAY 312

Query: 311 LEFYIRHTDGTPISSEAERQRVIQCLQASVERRTSQGVRLELCTTDRPCLLADVTRTFRE 370
            E+YIRHTDG+P+ SEAERQRVI+CL+A+++RR S+G++LELCT+DR  LL+DVTR FRE
Sbjct: 313 QEYYIRHTDGSPVKSEAERQRVIKCLKAAIQRRVSEGLKLELCTSDRVGLLSDVTRIFRE 372

Query: 371 NGLNVTRAEVSTSAEVALNLFYVTNAHGSAADAKTIESVREKIGLSSLKVKELPLISQQK 430
           N L VTRAEV T  + ALN FYV +A G   D KTIES+R+ IG + L+VK     +  K
Sbjct: 373 NSLTVTRAEVKTKGDKALNTFYVRDASGYQVDTKTIESIRQVIGQTILQVKGGN--TDAK 432

Query: 431 TEGEEQAAGVGGAVLLSLGSILRRNLYNLGLIRS 444
              ++   G         G    R+  N GLIRS
Sbjct: 433 PSPQDSPTG------FLFGVFKSRSFVNFGLIRS 456