Cp4.1LG13g01730 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG13g01730
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionProtein DETOXIFICATION
LocationCp4.1LG13: 1382965 .. 1384464 (-)
RNA-Seq ExpressionCp4.1LG13g01730
SyntenyCp4.1LG13g01730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTGTTCTGGTTCTGTACTTTCCGACCAGCCAAAGATTCCGACCAATGCTACTACTTCGCTTAATGCTCCTCTGATCTCCGAAACAGAGAGATTTCCAAATCTGTGTGTTGTTCTGACAGAGCTCAAATGCATAGCCGACATCGCACTTCCGATGATTCTTGTCGGCTTTTTGATGTACTCTCGTTCGATGATCTCCATGTTGTTTCTCGGCCGATTGGGTGGTTTGTCCTTGGCTGGTGGTTCGCTTGCGATTGGGTTTGCTAATATTACTGGCTACTCTGTTCTCTCTGGCCTCGCCGTCGGCATGGAGCCCATTTGCGGCCAAGCTTTTGGGGCTAAAAAATTCAAACTTTTGGGCCTTGCCCTTCAAAGAACAGTCATTCTTCTCCTCTTTTCGTCAATACCCATTTCTTTTTTGTGGTTTAACATGAAGAAAATCCTCCTCCTTTGTGGCCAAAACGATGATATTTCCAGTGAAGCTCATTCCTACATACTCTGTTCTGTCCCTGATTTAATTGCTCTGTCTTTTCTCCACCCTTTACGGATTTATCTTCGTAGTCAATCGATTAACCTCCCTCTCACATACTGCGCTGGATTAGCCATTATATTTCATATCCCAATCAATTATCTTCTCGTCTCTGTTTTCGATTTGGGAATTTACGGCGTCGCTTTGGGAGCTGTGTGGACCAATTTCAACCTTGTCGGATTACTGATTTTATTCATTGTACTCTCCGGCGTGTACGAGAAAACCTGGCCGGGAATGTCGTCGGAGTGTTTGAAGGAATGGAAATCGCTACTTGAATTGGCGATTCCGAGCTGTATTTCGGTGTGTTTAGAATGGTGGTGGTATGAAATCATGATTTTGCTAAGTGGGTTCATGATGAATCCTCAATCAACAGTGGCTTCCATGGGGATTTTGATACAAACCACTGCTTTGATCTACATTTTCCCTTCGTCATTAAGCTTTGGAGTATCAACCAGAGTGGGAAACGAATTGGGTGCAAATCGTCCAAACAAAGCCAAATTAGCAGCCATTGTTGGGCTCTGTACAAGCTTCTTCCTTGGGTTATCAGCTTTGTCGTTTGCTTTCAATGTCCGGAAAGTATGGGCGAAAATGTTCACAAATGACACAGGAATCATCGAATTGACATGTCTGGTTCTCCCAATCATCGGACTCTGCGAGCTGGGGAACTGCCCACAGACGACAAGCTGTGGAGTATTGAGGGGCACCGCGCGGCCAAAATTGGGGGCGAACATAAATTTGGGATGTTTTTACATGGTGGGCATGCCAATTGCTATATGGCTGAGCTTTTACGGCGGGTGGGACTTCAAAGGGCTGTGGATTGGGCTGTTGGCGGCGCAAGTGTCTTGTGCCATAGCGATGCTGATGGCTCTGGTTCGGACCAATTGGGAAGAACAGGCCGAGAGGGCTAAGGAGCTGACTGGAAGTGGGTCGGACGATGATGATGAAAAAACAGAGCTTTTGAATGCGTAG

mRNA sequence

ATGTCTTGTTCTGGTTCTGTACTTTCCGACCAGCCAAAGATTCCGACCAATGCTACTACTTCGCTTAATGCTCCTCTGATCTCCGAAACAGAGAGATTTCCAAATCTGTGTGTTGTTCTGACAGAGCTCAAATGCATAGCCGACATCGCACTTCCGATGATTCTTGTCGGCTTTTTGATGTACTCTCGTTCGATGATCTCCATGTTGTTTCTCGGCCGATTGGGTGGTTTGTCCTTGGCTGGTGGTTCGCTTGCGATTGGGTTTGCTAATATTACTGGCTACTCTGTTCTCTCTGGCCTCGCCGTCGGCATGGAGCCCATTTGCGGCCAAGCTTTTGGGGCTAAAAAATTCAAACTTTTGGGCCTTGCCCTTCAAAGAACAGTCATTCTTCTCCTCTTTTCGTCAATACCCATTTCTTTTTTGTGGTTTAACATGAAGAAAATCCTCCTCCTTTGTGGCCAAAACGATGATATTTCCAGTGAAGCTCATTCCTACATACTCTGTTCTGTCCCTGATTTAATTGCTCTGTCTTTTCTCCACCCTTTACGGATTTATCTTCGTAGTCAATCGATTAACCTCCCTCTCACATACTGCGCTGGATTAGCCATTATATTTCATATCCCAATCAATTATCTTCTCGTCTCTGTTTTCGATTTGGGAATTTACGGCGTCGCTTTGGGAGCTGTGTGGACCAATTTCAACCTTGTCGGATTACTGATTTTATTCATTGTACTCTCCGGCGTGTACGAGAAAACCTGGCCGGGAATGTCGTCGGAGTGTTTGAAGGAATGGAAATCGCTACTTGAATTGGCGATTCCGAGCTGTATTTCGGTGTGTTTAGAATGGTGGTGGTATGAAATCATGATTTTGCTAAGTGGGTTCATGATGAATCCTCAATCAACAGTGGCTTCCATGGGGATTTTGATACAAACCACTGCTTTGATCTACATTTTCCCTTCGTCATTAAGCTTTGGAGTATCAACCAGAGTGGGAAACGAATTGGGTGCAAATCGTCCAAACAAAGCCAAATTAGCAGCCATTGTTGGGCTCTGTACAAGCTTCTTCCTTGGGTTATCAGCTTTGTCGTTTGCTTTCAATGTCCGGAAAGTATGGGCGAAAATGTTCACAAATGACACAGGAATCATCGAATTGACATGTCTGGTTCTCCCAATCATCGGACTCTGCGAGCTGGGGAACTGCCCACAGACGACAAGCTGTGGAGTATTGAGGGGCACCGCGCGGCCAAAATTGGGGGCGAACATAAATTTGGGATGTTTTTACATGGTGGGCATGCCAATTGCTATATGGCTGAGCTTTTACGGCGGGTGGGACTTCAAAGGGCTGTGGATTGGGCTGTTGGCGGCGCAAGTGTCTTGTGCCATAGCGATGCTGATGGCTCTGGTTCGGACCAATTGGGAAGAACAGGCCGAGAGGGCTAAGGAGCTGACTGGAAGTGGGTCGGACGATGATGATGAAAAAACAGAGCTTTTGAATGCGTAG

Coding sequence (CDS)

ATGTCTTGTTCTGGTTCTGTACTTTCCGACCAGCCAAAGATTCCGACCAATGCTACTACTTCGCTTAATGCTCCTCTGATCTCCGAAACAGAGAGATTTCCAAATCTGTGTGTTGTTCTGACAGAGCTCAAATGCATAGCCGACATCGCACTTCCGATGATTCTTGTCGGCTTTTTGATGTACTCTCGTTCGATGATCTCCATGTTGTTTCTCGGCCGATTGGGTGGTTTGTCCTTGGCTGGTGGTTCGCTTGCGATTGGGTTTGCTAATATTACTGGCTACTCTGTTCTCTCTGGCCTCGCCGTCGGCATGGAGCCCATTTGCGGCCAAGCTTTTGGGGCTAAAAAATTCAAACTTTTGGGCCTTGCCCTTCAAAGAACAGTCATTCTTCTCCTCTTTTCGTCAATACCCATTTCTTTTTTGTGGTTTAACATGAAGAAAATCCTCCTCCTTTGTGGCCAAAACGATGATATTTCCAGTGAAGCTCATTCCTACATACTCTGTTCTGTCCCTGATTTAATTGCTCTGTCTTTTCTCCACCCTTTACGGATTTATCTTCGTAGTCAATCGATTAACCTCCCTCTCACATACTGCGCTGGATTAGCCATTATATTTCATATCCCAATCAATTATCTTCTCGTCTCTGTTTTCGATTTGGGAATTTACGGCGTCGCTTTGGGAGCTGTGTGGACCAATTTCAACCTTGTCGGATTACTGATTTTATTCATTGTACTCTCCGGCGTGTACGAGAAAACCTGGCCGGGAATGTCGTCGGAGTGTTTGAAGGAATGGAAATCGCTACTTGAATTGGCGATTCCGAGCTGTATTTCGGTGTGTTTAGAATGGTGGTGGTATGAAATCATGATTTTGCTAAGTGGGTTCATGATGAATCCTCAATCAACAGTGGCTTCCATGGGGATTTTGATACAAACCACTGCTTTGATCTACATTTTCCCTTCGTCATTAAGCTTTGGAGTATCAACCAGAGTGGGAAACGAATTGGGTGCAAATCGTCCAAACAAAGCCAAATTAGCAGCCATTGTTGGGCTCTGTACAAGCTTCTTCCTTGGGTTATCAGCTTTGTCGTTTGCTTTCAATGTCCGGAAAGTATGGGCGAAAATGTTCACAAATGACACAGGAATCATCGAATTGACATGTCTGGTTCTCCCAATCATCGGACTCTGCGAGCTGGGGAACTGCCCACAGACGACAAGCTGTGGAGTATTGAGGGGCACCGCGCGGCCAAAATTGGGGGCGAACATAAATTTGGGATGTTTTTACATGGTGGGCATGCCAATTGCTATATGGCTGAGCTTTTACGGCGGGTGGGACTTCAAAGGGCTGTGGATTGGGCTGTTGGCGGCGCAAGTGTCTTGTGCCATAGCGATGCTGATGGCTCTGGTTCGGACCAATTGGGAAGAACAGGCCGAGAGGGCTAAGGAGCTGACTGGAAGTGGGTCGGACGATGATGATGAAAAAACAGAGCTTTTGAATGCGTAG

Protein sequence

MSCSGSVLSDQPKIPTNATTSLNAPLISETERFPNLCVVLTELKCIADIALPMILVGFLMYSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFKLLGLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDISSEAHSYILCSVPDLIALSFLHPLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGLLILFIVLSGVYEKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSALSFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGANINLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAERAKELTGSGSDDDDEKTELLNA
Homology
BLAST of Cp4.1LG13g01730 vs. ExPASy Swiss-Prot
Match: O82752 (Protein DETOXIFICATION 49 OS=Arabidopsis thaliana OX=3702 GN=DTX49 PE=2 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 2.2e-178
Identity = 317/454 (69.82%), Postives = 378/454 (83.26%), Query Frame = 0

Query: 40  LTELKCIADIALPMILVGFLMYSRSMISMLFLGRLGGLS-LAGGSLAIGFANITGYSVLS 99
           + E K IA I+LP+IL G L+YSRSMISMLFLGRL  LS L+GGSLA+GFANITGYS+LS
Sbjct: 30  IQEAKSIAKISLPLILTGLLLYSRSMISMLFLGRLNDLSALSGGSLALGFANITGYSLLS 89

Query: 100 GLAVGMEPICGQAFGAKKFKLLGLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDI 159
           GL++GMEPIC QAFGAK+FKLLGLALQRT +LLL  S+PIS LW N+KKILL  GQ+++I
Sbjct: 90  GLSIGMEPICVQAFGAKRFKLLGLALQRTTLLLLLCSLPISILWLNIKKILLFFGQDEEI 149

Query: 160 SSEAHSYILCSVPDLIALSFLHPLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFD 219
           S++A  +IL S+PDLI  SFLHP+RIYLRSQSI LPLTY A  A++ HIPINYLLVS   
Sbjct: 150 SNQAEIFILFSLPDLILQSFLHPIRIYLRSQSITLPLTYSAFFAVLLHIPINYLLVSSLG 209

Query: 220 LGIYGVALGAVWTNFNLVGLLILFIVLSGVYEKTWPGMSSECLKEWKSLLELAIPSCISV 279
           LG+ GVALGA+WTN NL+G LI++IV SGVY+KTW G S +C K W+SL++LAIPSC+SV
Sbjct: 210 LGLKGVALGAIWTNVNLLGFLIIYIVFSGVYQKTWGGFSMDCFKGWRSLMKLAIPSCVSV 269

Query: 280 CLEWWWYEIMILLSGFMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANR 339
           CLEWWWYEIMILL G ++NPQ+TVASMGILIQTTALIYIFPSSLS  VSTRVGNELGAN+
Sbjct: 270 CLEWWWYEIMILLCGLLLNPQATVASMGILIQTTALIYIFPSSLSISVSTRVGNELGANQ 329

Query: 340 PNKAKLAAIVGLCTSFFLGLSALSFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELG 399
           P+KA++AA  GL  S  LGL A+ FA  VR  WA++FT++  I++LT +VLPIIGLCELG
Sbjct: 330 PDKARIAARTGLSLSLGLGLLAMFFALMVRNCWARLFTDEEEIVKLTSMVLPIIGLCELG 389

Query: 400 NCPQTTSCGVLRGTARPKLGANINLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQVS 459
           NCPQTT CGVLRG+ARPKLGANINL CFY VGMP+A+WLSF+ G+DFKGLW+GL AAQ S
Sbjct: 390 NCPQTTLCGVLRGSARPKLGANINLCCFYFVGMPVAVWLSFFSGFDFKGLWLGLFAAQGS 449

Query: 460 CAIAMLMALVRTNWEEQAERAKELTGSGSDDDDE 493
           C I+ML+ L RT+WE +  RAKEL     D D++
Sbjct: 450 CLISMLVVLARTDWEVEVHRAKELMTRSCDGDED 483

BLAST of Cp4.1LG13g01730 vs. ExPASy Swiss-Prot
Match: Q9SLV0 (Protein DETOXIFICATION 48 OS=Arabidopsis thaliana OX=3702 GN=DTX48 PE=2 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 9.2e-156
Identity = 289/492 (58.74%), Postives = 363/492 (73.78%), Query Frame = 0

Query: 2   SCSGSVLS--DQPKIPTNATTSLNAPLISE--------TERFPNLCVVLTELKCIADIAL 61
           S S S+LS  D+  I    T   + P  SE         +R+P+    L E+K I  I+ 
Sbjct: 8   SASSSLLSCKDKTHISKLETCDTDNPHYSEFRDTDSLDLKRWPSFLEGLEEVKAIGKISG 67

Query: 62  PMILVGFLMYSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQA 121
           P  + G LMYSR+MISMLFLG LG L LAGGSL+IGFANITGYSV+SGL++GMEPICGQA
Sbjct: 68  PTAMTGLLMYSRAMISMLFLGYLGELELAGGSLSIGFANITGYSVISGLSMGMEPICGQA 127

Query: 122 FGAKKFKLLGLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDISSEAHSYILCSVP 181
           +GAK+ KLLGL LQRTV+LLL  S+PISF W NM++ILL CGQ+++ISS A  ++L ++P
Sbjct: 128 YGAKQMKLLGLTLQRTVLLLLSCSVPISFSWLNMRRILLWCGQDEEISSVAQQFLLFAIP 187

Query: 182 DLIALSFLHPLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWT 241
           DL  LS LHPLRIYLR+Q+I LP+TY   ++++ H+P+NYLLV   ++G+ GVA+  V T
Sbjct: 188 DLFLLSLLHPLRIYLRTQNITLPVTYSTAVSVLLHVPLNYLLVVKLEMGVAGVAIAMVLT 247

Query: 242 NFNLVGLLILFIVLSGVYEKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILL 301
           N NLV LL  F+  + V+  TW  ++ + LK W +LL LAIP+C+SVCLEWWWYE MI+L
Sbjct: 248 NLNLVVLLSSFVYFTSVHSDTWVPITIDSLKGWSALLSLAIPTCVSVCLEWWWYEFMIIL 307

Query: 302 SGFMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLC 361
            G + NP++TVASMGILIQTTAL+Y+FPSSLS GVSTR+ NELGA RP KA+++ I+ L 
Sbjct: 308 CGLLANPRATVASMGILIQTTALVYVFPSSLSLGVSTRISNELGAKRPAKARVSMIISLF 367

Query: 362 TSFFLGLSALSFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRG 421
            +  LGL A+ FA  VR  W ++FT D  I++LT + LPI+GLCELGNCPQTT CGVLRG
Sbjct: 368 CAIALGLMAMVFAVLVRHHWGRLFTTDAEILQLTSIALPIVGLCELGNCPQTTGCGVLRG 427

Query: 422 TARPKLGANINLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTN 481
            ARP LGANINLG FY VGMP+AI   F     F GLW GLLAAQ +CA  ML AL+RT+
Sbjct: 428 CARPTLGANINLGSFYFVGMPVAILFGFVFKQGFPGLWFGLLAAQATCASLMLCALLRTD 487

Query: 482 WEEQAERAKELT 484
           W+ QAERA+ELT
Sbjct: 488 WKVQAERAEELT 499

BLAST of Cp4.1LG13g01730 vs. ExPASy Swiss-Prot
Match: Q4PSF4 (Protein DETOXIFICATION 52 OS=Arabidopsis thaliana OX=3702 GN=DTX52 PE=2 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 5.2e-143
Identity = 259/468 (55.34%), Postives = 339/468 (72.44%), Query Frame = 0

Query: 16  TNATTSLNAPLISETERFPNLCVVLTELKCIADIALPMILVGFLMYSRSMISMLFLGRLG 75
           TN  + ++    +    FP +  + +E + +  +A P IL   ++Y+RS ISMLFLG +G
Sbjct: 10  TNLLSKIDLEKQNPAPIFPTITELKSEARSLFSLAFPTILAALILYARSAISMLFLGHIG 69

Query: 76  GLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFKLLGLALQRTVILLLFSS 135
            L LAGGSLAI FANITGYSVL+GLA+GM+P+C QAFGA + KLL L LQRTV+ LL SS
Sbjct: 70  ELELAGGSLAIAFANITGYSVLAGLALGMDPLCSQAFGAGRPKLLSLTLQRTVLFLLTSS 129

Query: 136 IPISFLWFNMKKILLLCGQNDDISSEAHSYILCSVPDLIALSFLHPLRIYLRSQSINLPL 195
           + I  LW N+ KI++   Q+  ISS A +YILCS+PDL+  SFLHPLRIYLR+Q I  PL
Sbjct: 130 VVIVALWLNLGKIMIYLHQDPSISSLAQTYILCSIPDLLTNSFLHPLRIYLRAQGITSPL 189

Query: 196 TYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGLLILFIVLSGVYEKTWPG 255
           T       IFHIP+N+ LVS    G  GV++ A  +N  +V  L+  + ++G+++ TW  
Sbjct: 190 TLATLAGTIFHIPMNFFLVSYLGWGFMGVSMAAAASNLLVVIFLVAHVWIAGLHQPTWTR 249

Query: 256 MSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNPQSTVASMGILIQTTALI 315
            SSEC K+W  ++ LAIPSCI VCLEWWWYEIM +L G +++P + VASMGILIQTT+L+
Sbjct: 250 PSSECFKDWGPVVTLAIPSCIGVCLEWWWYEIMTVLCGLLIDPSTPVASMGILIQTTSLL 309

Query: 316 YIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSALSFAFNVRKVWAKMF 375
           YIFPSSL   VSTRVGNELG+NRPNKA+L+AIV +  +  +GL+A +FA+ V  VW  +F
Sbjct: 310 YIFPSSLGLAVSTRVGNELGSNRPNKARLSAIVAVSFAGVMGLTASAFAWGVSDVWGWIF 369

Query: 376 TNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGANINLGCFYMVGMPIAI 435
           TND  II+LT   LPI+GLCELGNCPQT  CGV+RGTARP + ANINLG FY+VG P+A+
Sbjct: 370 TNDVAIIKLTAAALPILGLCELGNCPQTVGCGVVRGTARPSMAANINLGAFYLVGTPVAV 429

Query: 436 WLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAERAKELT 484
            L+F+  + F GLW+GLLAAQ+ CA  ML  +  T+WE++A RA++LT
Sbjct: 430 GLTFWAAYGFCGLWVGLLAAQICCAAMMLYVVATTDWEKEAIRARKLT 477

BLAST of Cp4.1LG13g01730 vs. ExPASy Swiss-Prot
Match: Q9FJ87 (Protein DETOXIFICATION 50 OS=Arabidopsis thaliana OX=3702 GN=DTX50 PE=2 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 1.7e-141
Identity = 267/485 (55.05%), Postives = 349/485 (71.96%), Query Frame = 0

Query: 25  PLISETERFPN----LCVVLTELKCIADIALPMILVGFLMYSRSMISMLFLGRLGGLSLA 84
           PL+ +T    N    L V L E   I  I+ P++L G  +Y RS +S+ FLG LG  +LA
Sbjct: 14  PLLQKTSHLKNHSSVLSVFLNEAISICKISYPLVLTGLFLYVRSFVSLSFLGGLGDATLA 73

Query: 85  GGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFKLLGLALQRTVILLLFSSIPISF 144
           GGSLA  FANITGYS+ SGL +G+E IC QAFGA+++  +  +++R +ILLL +S+P++ 
Sbjct: 74  GGSLAAAFANITGYSLFSGLTMGVESICSQAFGARRYNYVCASVKRGIILLLVTSLPVTL 133

Query: 145 LWFNMKKILLLCGQNDDISSEAHSYILCSVPDLIALSFLHPLRIYLRSQSINLPLTYCAG 204
           LW NM+KILL+  Q+  ++SEAH ++L SVPDL+A SFLHPLR+YLR+QS  LPL+ C  
Sbjct: 134 LWMNMEKILLILKQDKKLASEAHIFLLYSVPDLVAQSFLHPLRVYLRTQSKTLPLSICTV 193

Query: 205 LAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGLLILFI------VLSGVYEKTWP 264
           +A   H+PI + LVS   LGI G+AL  V +NFNLV  L L+I      +     EK   
Sbjct: 194 IASFLHLPITFFLVSYLGLGIKGIALSGVVSNFNLVAFLFLYICFFEDKLSVNEDEKITE 253

Query: 265 GMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNPQSTVASMGILIQTTAL 324
               + ++EWK LL LAIPSCISVCLEWW YEIMILL GF+++P+++VASMGILIQ T+L
Sbjct: 254 ETCEDSVREWKKLLCLAIPSCISVCLEWWCYEIMILLCGFLLDPKASVASMGILIQITSL 313

Query: 325 IYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSALSFAFNVRKVWAKM 384
           +YIFP SLS GVSTRVGNELG+N+P +A+ AAIVGL  S  LG +A +F  +VR  WA  
Sbjct: 314 VYIFPHSLSLGVSTRVGNELGSNQPKRARRAAIVGLGLSIALGFTAFAFTVSVRNTWAMF 373

Query: 385 FTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGANINLGCFYMVGMPIA 444
           FT+D  I++LT + LPI+GLCELGNCPQTT CGVLRG+ARPK+GANIN   FY VG+P+ 
Sbjct: 374 FTDDKEIMKLTAMALPIVGLCELGNCPQTTGCGVLRGSARPKIGANINGVAFYAVGIPVG 433

Query: 445 IWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAERAKELTGS---GSDDDD 497
             L+F+ G+ FKGLW+G+LAAQ++C I M+ A  RT+WE +AERAK LT +   GS DDD
Sbjct: 434 AVLAFWFGFGFKGLWLGMLAAQITCVIGMMAATCRTDWELEAERAKVLTTAVDCGSSDDD 493

BLAST of Cp4.1LG13g01730 vs. ExPASy Swiss-Prot
Match: Q9SZE2 (Protein DETOXIFICATION 51 OS=Arabidopsis thaliana OX=3702 GN=DTX51 PE=2 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 3.9e-138
Identity = 246/463 (53.13%), Postives = 330/463 (71.27%), Query Frame = 0

Query: 29  ETERFPNLCVVLTELKCIADIALPMILVGFLMYSRSMISMLFLGRLGGLSLAGGSLAIGF 88
           E    P +   +TE K +  +A P+ +   ++Y RS +SM FLG+LG L LA GSLAI F
Sbjct: 43  ENRGSPLMAEAVTEAKSLFTLAFPIAVTALVLYLRSAVSMFFLGQLGDLELAAGSLAIAF 102

Query: 89  ANITGYSVLSGLAVGMEPICGQAFGAKKFKLLGLALQRTVILLLFSSIPISFLWFNMKKI 148
           ANITGYSVLSGLA+GMEP+C QAFGA +FKLL L L RTV+ LL   +PIS LWFN+ KI
Sbjct: 103 ANITGYSVLSGLALGMEPLCSQAFGAHRFKLLSLTLHRTVVFLLVCCVPISVLWFNVGKI 162

Query: 149 LLLCGQNDDISSEAHSYILCSVPDLIALSFLHPLRIYLRSQSINLPLTYCAGLAIIFHIP 208
            +   Q+ DI+  A +Y++ S+PDL+  + LHP+RIYLR+Q I  P+T  +    +FH+P
Sbjct: 163 SVYLHQDPDIAKLAQTYLIFSLPDLLTNTLLHPIRIYLRAQGIIHPVTLASLSGAVFHLP 222

Query: 209 INYLLVSVFDLGIYGVALGAVWTNFNLVGLLILFIVLSGVYEKTWPGMSSECLKEWKSLL 268
            N  LVS   LG+ GVA+ +  TN  +V  L+ ++  SG++  TW   + +C + W  LL
Sbjct: 223 ANLFLVSYLRLGLTGVAVASSITNIFVVAFLVCYVWASGLHAPTWTDPTRDCFRGWAPLL 282

Query: 269 ELAIPSCISVCLEWWWYEIMILLSGFMMNPQSTVASMGILIQTTALIYIFPSSLSFGVST 328
            LA PSC+SVCLEWWWYEIMI+L G ++NP+STVA+MG+LIQTT+ +Y+FPSSLSF VST
Sbjct: 283 RLAGPSCVSVCLEWWWYEIMIVLCGLLVNPRSTVAAMGVLIQTTSFLYVFPSSLSFAVST 342

Query: 329 RVGNELGANRPNKAKLAAIVGLCTSFFLGLSALSFAFNVRKVWAKMFTNDTGIIELTCLV 388
           RVGNELGANRP  AKL A V +  +   G+ A +FA++VR  W ++FT D  I++LT   
Sbjct: 343 RVGNELGANRPKTAKLTATVAIVFAAVTGIIAAAFAYSVRNAWGRIFTGDKEILQLTAAA 402

Query: 389 LPIIGLCELGNCPQTTSCGVLRGTARPKLGANINLGCFYMVGMPIAIWLSFYGGWDFKGL 448
           LPI+GLCE+GNCPQT  CGV+RGTARP   AN+NLG FY+VGMP+A+ L F+ G  F GL
Sbjct: 403 LPILGLCEIGNCPQTVGCGVVRGTARPSTAANVNLGAFYLVGMPVAVGLGFWAGIGFNGL 462

Query: 449 WIGLLAAQVSCAIAMLMALVRTNWEEQAERAKELTGSGSDDDD 492
           W+GLLAAQ+SCA  M+  +  T+WE +A++A+ LT + + ++D
Sbjct: 463 WVGLLAAQISCAGLMMYVVGTTDWESEAKKAQTLTCAETVEND 505

BLAST of Cp4.1LG13g01730 vs. NCBI nr
Match: XP_023551348.1 (protein DETOXIFICATION 49-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 972 bits (2513), Expect = 0.0
Identity = 499/499 (100.00%), Postives = 499/499 (100.00%), Query Frame = 0

Query: 1   MSCSGSVLSDQPKIPTNATTSLNAPLISETERFPNLCVVLTELKCIADIALPMILVGFLM 60
           MSCSGSVLSDQPKIPTNATTSLNAPLISETERFPNLCVVLTELKCIADIALPMILVGFLM
Sbjct: 1   MSCSGSVLSDQPKIPTNATTSLNAPLISETERFPNLCVVLTELKCIADIALPMILVGFLM 60

Query: 61  YSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFKLL 120
           YSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFKLL
Sbjct: 61  YSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFKLL 120

Query: 121 GLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDISSEAHSYILCSVPDLIALSFLH 180
           GLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDISSEAHSYILCSVPDLIALSFLH
Sbjct: 121 GLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDISSEAHSYILCSVPDLIALSFLH 180

Query: 181 PLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGLLI 240
           PLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGLLI
Sbjct: 181 PLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGLLI 240

Query: 241 LFIVLSGVYEKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNPQS 300
           LFIVLSGVYEKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNPQS
Sbjct: 241 LFIVLSGVYEKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNPQS 300

Query: 301 TVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSA 360
           TVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSA
Sbjct: 301 TVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSA 360

Query: 361 LSFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGAN 420
           LSFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGAN
Sbjct: 361 LSFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGAN 420

Query: 421 INLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAERAK 480
           INLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAERAK
Sbjct: 421 INLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAERAK 480

Query: 481 ELTGSGSDDDDEKTELLNA 499
           ELTGSGSDDDDEKTELLNA
Sbjct: 481 ELTGSGSDDDDEKTELLNA 499

BLAST of Cp4.1LG13g01730 vs. NCBI nr
Match: KAG7016948.1 (Protein DETOXIFICATION 49, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 961 bits (2485), Expect = 0.0
Identity = 493/499 (98.80%), Postives = 497/499 (99.60%), Query Frame = 0

Query: 1   MSCSGSVLSDQPKIPTNATTSLNAPLISETERFPNLCVVLTELKCIADIALPMILVGFLM 60
           MS SGSVLSDQPKIPTN TTSLNAPLISETERFPNLCVVLTELKCIADIALPMILVGFLM
Sbjct: 1   MSGSGSVLSDQPKIPTNTTTSLNAPLISETERFPNLCVVLTELKCIADIALPMILVGFLM 60

Query: 61  YSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFKLL 120
           YSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFKLL
Sbjct: 61  YSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFKLL 120

Query: 121 GLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDISSEAHSYILCSVPDLIALSFLH 180
           GLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDI+SEAHSYILCSVPDLIALSFLH
Sbjct: 121 GLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDIASEAHSYILCSVPDLIALSFLH 180

Query: 181 PLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGLLI 240
           PLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGLLI
Sbjct: 181 PLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGLLI 240

Query: 241 LFIVLSGVYEKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNPQS 300
           LFIV+SGVYEKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNPQS
Sbjct: 241 LFIVVSGVYEKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNPQS 300

Query: 301 TVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSA 360
           TVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSA
Sbjct: 301 TVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSA 360

Query: 361 LSFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGAN 420
           L+FAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGAN
Sbjct: 361 LAFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGAN 420

Query: 421 INLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAERAK 480
           INLGCFY+VGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAERAK
Sbjct: 421 INLGCFYLVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAERAK 480

Query: 481 ELTGSGSDDDDEKTELLNA 499
           ELTGSGSDDDDEKTELLNA
Sbjct: 481 ELTGSGSDDDDEKTELLNA 499

BLAST of Cp4.1LG13g01730 vs. NCBI nr
Match: KAG6579480.1 (Protein DETOXIFICATION 49, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 959 bits (2480), Expect = 0.0
Identity = 492/499 (98.60%), Postives = 497/499 (99.60%), Query Frame = 0

Query: 1   MSCSGSVLSDQPKIPTNATTSLNAPLISETERFPNLCVVLTELKCIADIALPMILVGFLM 60
           MS SGSVLSDQPKIPTN TTSLNAPLISETERFPNLCVVLTELKCIADIALPMILVGFLM
Sbjct: 1   MSGSGSVLSDQPKIPTNTTTSLNAPLISETERFPNLCVVLTELKCIADIALPMILVGFLM 60

Query: 61  YSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFKLL 120
           YSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFKLL
Sbjct: 61  YSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFKLL 120

Query: 121 GLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDISSEAHSYILCSVPDLIALSFLH 180
           GLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDI+SEAHSYILCSVPDLIALSFLH
Sbjct: 121 GLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDIASEAHSYILCSVPDLIALSFLH 180

Query: 181 PLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGLLI 240
           PLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGLLI
Sbjct: 181 PLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGLLI 240

Query: 241 LFIVLSGVYEKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNPQS 300
           LFIV+SGVYEKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNPQS
Sbjct: 241 LFIVVSGVYEKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNPQS 300

Query: 301 TVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSA 360
           TVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRP+KAKLAAIVGLCTSFFLGLSA
Sbjct: 301 TVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPSKAKLAAIVGLCTSFFLGLSA 360

Query: 361 LSFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGAN 420
           L+FAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGAN
Sbjct: 361 LAFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGAN 420

Query: 421 INLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAERAK 480
           INLGCFY+VGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAERAK
Sbjct: 421 INLGCFYLVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAERAK 480

Query: 481 ELTGSGSDDDDEKTELLNA 499
           ELTGSGSDDDDEKTELLNA
Sbjct: 481 ELTGSGSDDDDEKTELLNA 499

BLAST of Cp4.1LG13g01730 vs. NCBI nr
Match: XP_022922331.1 (protein DETOXIFICATION 49-like [Cucurbita moschata])

HSP 1 Score: 957 bits (2473), Expect = 0.0
Identity = 489/499 (98.00%), Postives = 496/499 (99.40%), Query Frame = 0

Query: 1   MSCSGSVLSDQPKIPTNATTSLNAPLISETERFPNLCVVLTELKCIADIALPMILVGFLM 60
           MS SGSVLSDQPKIPTN TTSLNAPLISETERFPNLCVVLTELKCIADIALPMILVGFL+
Sbjct: 1   MSGSGSVLSDQPKIPTNTTTSLNAPLISETERFPNLCVVLTELKCIADIALPMILVGFLI 60

Query: 61  YSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFKLL 120
           YSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFKLL
Sbjct: 61  YSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFKLL 120

Query: 121 GLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDISSEAHSYILCSVPDLIALSFLH 180
           GLALQRTV+LLLFSS+PISFLWFNMKKILLLCGQNDDI+SEAHSYILCSVPDLIALSFLH
Sbjct: 121 GLALQRTVVLLLFSSVPISFLWFNMKKILLLCGQNDDIASEAHSYILCSVPDLIALSFLH 180

Query: 181 PLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGLLI 240
           PLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGLLI
Sbjct: 181 PLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGLLI 240

Query: 241 LFIVLSGVYEKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNPQS 300
           LFIV+SGVYE TWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFM+NPQS
Sbjct: 241 LFIVVSGVYENTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMINPQS 300

Query: 301 TVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSA 360
           TVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSA
Sbjct: 301 TVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSA 360

Query: 361 LSFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGAN 420
           L+FAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGAN
Sbjct: 361 LAFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGAN 420

Query: 421 INLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAERAK 480
           INLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAERAK
Sbjct: 421 INLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAERAK 480

Query: 481 ELTGSGSDDDDEKTELLNA 499
           ELTGSGSDDDDEKTELLNA
Sbjct: 481 ELTGSGSDDDDEKTELLNA 499

BLAST of Cp4.1LG13g01730 vs. NCBI nr
Match: XP_022970176.1 (protein DETOXIFICATION 49-like [Cucurbita maxima])

HSP 1 Score: 939 bits (2428), Expect = 0.0
Identity = 481/496 (96.98%), Postives = 492/496 (99.19%), Query Frame = 0

Query: 4   SGSVLSDQPKIPTNATTSLNAPLISETERFPNLCVVLTELKCIADIALPMILVGFLMYSR 63
           SGSVLSDQPKIPTN TTSL APLISETERFPNLC+VLTELKCIADIALPM+LVGFLMYSR
Sbjct: 8   SGSVLSDQPKIPTN-TTSLKAPLISETERFPNLCLVLTELKCIADIALPMVLVGFLMYSR 67

Query: 64  SMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFKLLGLA 123
           SMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGA+KFKLLGLA
Sbjct: 68  SMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAQKFKLLGLA 127

Query: 124 LQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDISSEAHSYILCSVPDLIALSFLHPLR 183
           LQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDI+SEAHSYILCSVPDLIALSFLHPLR
Sbjct: 128 LQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDIASEAHSYILCSVPDLIALSFLHPLR 187

Query: 184 IYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGLLILFI 243
           IYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFD GIYGVALGAVWTNFNLVGLLILFI
Sbjct: 188 IYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDWGIYGVALGAVWTNFNLVGLLILFI 247

Query: 244 VLSGVYEKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNPQSTVA 303
           ++SGVY+KTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNPQSTVA
Sbjct: 248 IVSGVYKKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNPQSTVA 307

Query: 304 SMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSALSF 363
           SMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSAL+F
Sbjct: 308 SMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSALAF 367

Query: 364 AFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGANINL 423
           AFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGANINL
Sbjct: 368 AFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGANINL 427

Query: 424 GCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAERAKELT 483
           GCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQ SCAIAMLMALVRTNWEE+AERAKELT
Sbjct: 428 GCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQGSCAIAMLMALVRTNWEEEAERAKELT 487

Query: 484 GSGSDDDDEKTELLNA 499
           GSGSDD+DEKTELLN+
Sbjct: 488 GSGSDDNDEKTELLNS 502

BLAST of Cp4.1LG13g01730 vs. ExPASy TrEMBL
Match: A0A6J1E3V0 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111430342 PE=3 SV=1)

HSP 1 Score: 957 bits (2473), Expect = 0.0
Identity = 489/499 (98.00%), Postives = 496/499 (99.40%), Query Frame = 0

Query: 1   MSCSGSVLSDQPKIPTNATTSLNAPLISETERFPNLCVVLTELKCIADIALPMILVGFLM 60
           MS SGSVLSDQPKIPTN TTSLNAPLISETERFPNLCVVLTELKCIADIALPMILVGFL+
Sbjct: 1   MSGSGSVLSDQPKIPTNTTTSLNAPLISETERFPNLCVVLTELKCIADIALPMILVGFLI 60

Query: 61  YSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFKLL 120
           YSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFKLL
Sbjct: 61  YSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFKLL 120

Query: 121 GLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDISSEAHSYILCSVPDLIALSFLH 180
           GLALQRTV+LLLFSS+PISFLWFNMKKILLLCGQNDDI+SEAHSYILCSVPDLIALSFLH
Sbjct: 121 GLALQRTVVLLLFSSVPISFLWFNMKKILLLCGQNDDIASEAHSYILCSVPDLIALSFLH 180

Query: 181 PLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGLLI 240
           PLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGLLI
Sbjct: 181 PLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGLLI 240

Query: 241 LFIVLSGVYEKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNPQS 300
           LFIV+SGVYE TWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFM+NPQS
Sbjct: 241 LFIVVSGVYENTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMINPQS 300

Query: 301 TVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSA 360
           TVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSA
Sbjct: 301 TVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSA 360

Query: 361 LSFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGAN 420
           L+FAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGAN
Sbjct: 361 LAFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGAN 420

Query: 421 INLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAERAK 480
           INLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAERAK
Sbjct: 421 INLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAERAK 480

Query: 481 ELTGSGSDDDDEKTELLNA 499
           ELTGSGSDDDDEKTELLNA
Sbjct: 481 ELTGSGSDDDDEKTELLNA 499

BLAST of Cp4.1LG13g01730 vs. ExPASy TrEMBL
Match: A0A6J1HZW9 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111469149 PE=3 SV=1)

HSP 1 Score: 939 bits (2428), Expect = 0.0
Identity = 481/496 (96.98%), Postives = 492/496 (99.19%), Query Frame = 0

Query: 4   SGSVLSDQPKIPTNATTSLNAPLISETERFPNLCVVLTELKCIADIALPMILVGFLMYSR 63
           SGSVLSDQPKIPTN TTSL APLISETERFPNLC+VLTELKCIADIALPM+LVGFLMYSR
Sbjct: 8   SGSVLSDQPKIPTN-TTSLKAPLISETERFPNLCLVLTELKCIADIALPMVLVGFLMYSR 67

Query: 64  SMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFKLLGLA 123
           SMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGA+KFKLLGLA
Sbjct: 68  SMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAQKFKLLGLA 127

Query: 124 LQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDISSEAHSYILCSVPDLIALSFLHPLR 183
           LQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDI+SEAHSYILCSVPDLIALSFLHPLR
Sbjct: 128 LQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDIASEAHSYILCSVPDLIALSFLHPLR 187

Query: 184 IYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGLLILFI 243
           IYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFD GIYGVALGAVWTNFNLVGLLILFI
Sbjct: 188 IYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDWGIYGVALGAVWTNFNLVGLLILFI 247

Query: 244 VLSGVYEKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNPQSTVA 303
           ++SGVY+KTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNPQSTVA
Sbjct: 248 IVSGVYKKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNPQSTVA 307

Query: 304 SMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSALSF 363
           SMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSAL+F
Sbjct: 308 SMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSALAF 367

Query: 364 AFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGANINL 423
           AFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGANINL
Sbjct: 368 AFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGANINL 427

Query: 424 GCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAERAKELT 483
           GCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQ SCAIAMLMALVRTNWEE+AERAKELT
Sbjct: 428 GCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQGSCAIAMLMALVRTNWEEEAERAKELT 487

Query: 484 GSGSDDDDEKTELLNA 499
           GSGSDD+DEKTELLN+
Sbjct: 488 GSGSDDNDEKTELLNS 502

BLAST of Cp4.1LG13g01730 vs. ExPASy TrEMBL
Match: A0A6J1H5E0 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111459742 PE=3 SV=1)

HSP 1 Score: 792 bits (2045), Expect = 2.11e-285
Identity = 404/488 (82.79%), Postives = 439/488 (89.96%), Query Frame = 0

Query: 7   VLSDQPKIPTNATTSLNAPLISET--------ERFPNLCVVLTELKCIADIALPMILVGF 66
           +LSDQP  PT    SL APLISE         +++P LC++L+E K IA +ALPMI VGF
Sbjct: 8   LLSDQPNSPTT-NHSLTAPLISENPTISTTVADKYPFLCLLLSECKSIAHVALPMIFVGF 67

Query: 67  LMYSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFK 126
            MYSRSMISMLFLG+LG LSLAGGSLAIGFANITGYSVLSGLA GMEPICGQAFGAK+FK
Sbjct: 68  FMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAFGAKRFK 127

Query: 127 LLGLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDISSEAHSYILCSVPDLIALSF 186
           LLGLALQRTVILLL SSIPISFLW NMKKIL+ CGQ DDI++EAHS+ILCS+PDLIALSF
Sbjct: 128 LLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPDLIALSF 187

Query: 187 LHPLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGL 246
           LHPLRIYLR QSINLPLTYC GL+++FH+PINYLLVSV DLGI+GVALGAVWTNFNLVG 
Sbjct: 188 LHPLRIYLRCQSINLPLTYCVGLSVLFHVPINYLLVSVLDLGIHGVALGAVWTNFNLVGS 247

Query: 247 LILFIVLSGVYEKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNP 306
           +I+FI++ GVY+ +WPG SSECLKEWKSLL LAIPSC+SVCLEWWWYEIMI+LSGFMMNP
Sbjct: 248 VIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLSGFMMNP 307

Query: 307 QSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGL 366
           QSTVASMGILIQTTALIYIFPSSLS GVSTRVGNELGAN+PN+AKLAAIVGLC SFF GL
Sbjct: 308 QSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCFSFFFGL 367

Query: 367 SALSFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLG 426
           SAL FAF VRKVWA MFTNDT IIELT LVLPIIGLCELGNCPQTT+CGVLRGTARP LG
Sbjct: 368 SALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGTARPTLG 427

Query: 427 ANINLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAER 486
           ANINL CFY+VGMPIAIWLSFYGGWDFKGLWIGLLAAQ SCA+AMLMAL RTNWEEQAER
Sbjct: 428 ANINLVCFYIVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNWEEQAER 487

BLAST of Cp4.1LG13g01730 vs. ExPASy TrEMBL
Match: A0A0A0KKM4 (Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_5G155540 PE=3 SV=1)

HSP 1 Score: 789 bits (2037), Expect = 4.04e-284
Identity = 405/483 (83.85%), Postives = 436/483 (90.27%), Query Frame = 0

Query: 21  SLNAPLISETE-RFP-NLCVVLTELKCIADIALPMILVGFLMYSRSMISMLFLGRLGGLS 80
           +L  PLI ETE +FP     VL+E K IADIA PMILVGFLMYSRSMISMLFLGRLGGLS
Sbjct: 12  TLKTPLIEETETKFPYRFSHVLSEAKSIADIAFPMILVGFLMYSRSMISMLFLGRLGGLS 71

Query: 81  LAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFKLLGLALQRTVILLLFSSIPI 140
           LAGGSLAIGFANITGYS+LSGLA+GMEPICGQAFGAK+FKLLGL LQRT+ILLL SS+PI
Sbjct: 72  LAGGSLAIGFANITGYSLLSGLAMGMEPICGQAFGAKRFKLLGLTLQRTIILLLISSLPI 131

Query: 141 SFLWFNMKKILLLCGQNDDISSEAHSYILCSVPDLIALSFLHPLRIYLRSQSINLPLTYC 200
           SFLWFNMKKILL CGQ+ DI++EAHSYILCS+PDL+ALSFLHPLRIYLRSQSINLPLTYC
Sbjct: 132 SFLWFNMKKILLFCGQDVDIANEAHSYILCSLPDLVALSFLHPLRIYLRSQSINLPLTYC 191

Query: 201 AGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGLLILFIVLSGVYEKTWPGMSS 260
           A LAI+FHIPINY  V VF+ GI GVALGAVWTNFN VG L++F++ SGVY+KTWPGMSS
Sbjct: 192 AILAILFHIPINYFFVCVFEWGIRGVALGAVWTNFNFVGSLVVFVLFSGVYKKTWPGMSS 251

Query: 261 ECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNPQSTVASMGILIQTTALIYIF 320
           +CLKEWKSLL LAIPSCISVCLEWWWYEIMILLSGFM+NPQSTVASMGILIQTTALIYIF
Sbjct: 252 DCLKEWKSLLGLAIPSCISVCLEWWWYEIMILLSGFMLNPQSTVASMGILIQTTALIYIF 311

Query: 321 PSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSALSFAFNVRKVWAKMFTND 380
           PSSLSFGVSTRVGNELGAN PNKAKLAAIVGLC SFFLG+SAL FAF +RKVWA MFT D
Sbjct: 312 PSSLSFGVSTRVGNELGANHPNKAKLAAIVGLCISFFLGISALLFAFKIRKVWATMFTED 371

Query: 381 TGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGANINLGCFYMVGMPIAIWLS 440
             IIELT L+LPIIGLCELGNCPQTTSCGVLRGTARPKLGANINLGCFYMVGMP+AIWLS
Sbjct: 372 IQIIELTSLILPIIGLCELGNCPQTTSCGVLRGTARPKLGANINLGCFYMVGMPVAIWLS 431

Query: 441 FYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAERAKELTGSGSD---DDDEKTE 498
           FYGGWDFKGLWIGLLAAQ SCA+ MLM L RTNWEEQAERAKELT +G +   DD+E+ E
Sbjct: 432 FYGGWDFKGLWIGLLAAQASCAMTMLMVLTRTNWEEQAERAKELTKNGLEEIEDDEEEEE 491

BLAST of Cp4.1LG13g01730 vs. ExPASy TrEMBL
Match: A0A6J1K4X4 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111491221 PE=3 SV=1)

HSP 1 Score: 782 bits (2020), Expect = 1.35e-281
Identity = 401/488 (82.17%), Postives = 434/488 (88.93%), Query Frame = 0

Query: 7   VLSDQPKIPTNATTSLNAPLISET--------ERFPNLCVVLTELKCIADIALPMILVGF 66
           +LSDQP  PT    SL APLISE         +++P LC+VL+E K IA +ALPMI VGF
Sbjct: 8   LLSDQPNSPTT-NHSLTAPLISENPTISTTVADKYPFLCLVLSECKSIAHVALPMIFVGF 67

Query: 67  LMYSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFK 126
            MYSRSMISMLFLG+LG LSLAGGSLAIGFANITGYSVLSGLA GMEPICGQAFGAK+FK
Sbjct: 68  FMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAFGAKRFK 127

Query: 127 LLGLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDISSEAHSYILCSVPDLIALSF 186
           LLGLALQRTVILLL SSIPISF W NMKKIL+ CGQ DDI++EA S+ILCS+PDLIALSF
Sbjct: 128 LLGLALQRTVILLLLSSIPISFSWLNMKKILIFCGQEDDIANEAQSFILCSIPDLIALSF 187

Query: 187 LHPLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGL 246
           LHPLRIYLR QSINLPLTYC  L+++ H+PINYLLVSV DLGI+GVALGAVWTNFNLVG 
Sbjct: 188 LHPLRIYLRCQSINLPLTYCVALSVLLHVPINYLLVSVLDLGIHGVALGAVWTNFNLVGS 247

Query: 247 LILFIVLSGVYEKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNP 306
           +I+FI++ GVY+ +WPG SSECLKEWKSLL LAIPSCISVCLEWWWYEIM++LSGFMMNP
Sbjct: 248 VIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCISVCLEWWWYEIMVMLSGFMMNP 307

Query: 307 QSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGL 366
           QSTVASMGILIQTTALIYIFPSSLS GVSTRVG ELGAN+PN+AKLAAIVGLC SFF GL
Sbjct: 308 QSTVASMGILIQTTALIYIFPSSLSVGVSTRVGKELGANQPNRAKLAAIVGLCFSFFFGL 367

Query: 367 SALSFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLG 426
           SAL FAF VRKVWA MFTNDT IIELT LVLPIIGLCELGNCPQTT+CGVLRGTARP LG
Sbjct: 368 SALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGTARPTLG 427

Query: 427 ANINLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAER 486
           ANINL CFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQ SCA+AMLMAL RTNWEEQAER
Sbjct: 428 ANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNWEEQAER 487

BLAST of Cp4.1LG13g01730 vs. TAIR 10
Match: AT4G23030.1 (MATE efflux family protein )

HSP 1 Score: 626.7 bits (1615), Expect = 1.6e-179
Identity = 317/454 (69.82%), Postives = 378/454 (83.26%), Query Frame = 0

Query: 40  LTELKCIADIALPMILVGFLMYSRSMISMLFLGRLGGLS-LAGGSLAIGFANITGYSVLS 99
           + E K IA I+LP+IL G L+YSRSMISMLFLGRL  LS L+GGSLA+GFANITGYS+LS
Sbjct: 30  IQEAKSIAKISLPLILTGLLLYSRSMISMLFLGRLNDLSALSGGSLALGFANITGYSLLS 89

Query: 100 GLAVGMEPICGQAFGAKKFKLLGLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDI 159
           GL++GMEPIC QAFGAK+FKLLGLALQRT +LLL  S+PIS LW N+KKILL  GQ+++I
Sbjct: 90  GLSIGMEPICVQAFGAKRFKLLGLALQRTTLLLLLCSLPISILWLNIKKILLFFGQDEEI 149

Query: 160 SSEAHSYILCSVPDLIALSFLHPLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFD 219
           S++A  +IL S+PDLI  SFLHP+RIYLRSQSI LPLTY A  A++ HIPINYLLVS   
Sbjct: 150 SNQAEIFILFSLPDLILQSFLHPIRIYLRSQSITLPLTYSAFFAVLLHIPINYLLVSSLG 209

Query: 220 LGIYGVALGAVWTNFNLVGLLILFIVLSGVYEKTWPGMSSECLKEWKSLLELAIPSCISV 279
           LG+ GVALGA+WTN NL+G LI++IV SGVY+KTW G S +C K W+SL++LAIPSC+SV
Sbjct: 210 LGLKGVALGAIWTNVNLLGFLIIYIVFSGVYQKTWGGFSMDCFKGWRSLMKLAIPSCVSV 269

Query: 280 CLEWWWYEIMILLSGFMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANR 339
           CLEWWWYEIMILL G ++NPQ+TVASMGILIQTTALIYIFPSSLS  VSTRVGNELGAN+
Sbjct: 270 CLEWWWYEIMILLCGLLLNPQATVASMGILIQTTALIYIFPSSLSISVSTRVGNELGANQ 329

Query: 340 PNKAKLAAIVGLCTSFFLGLSALSFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELG 399
           P+KA++AA  GL  S  LGL A+ FA  VR  WA++FT++  I++LT +VLPIIGLCELG
Sbjct: 330 PDKARIAARTGLSLSLGLGLLAMFFALMVRNCWARLFTDEEEIVKLTSMVLPIIGLCELG 389

Query: 400 NCPQTTSCGVLRGTARPKLGANINLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQVS 459
           NCPQTT CGVLRG+ARPKLGANINL CFY VGMP+A+WLSF+ G+DFKGLW+GL AAQ S
Sbjct: 390 NCPQTTLCGVLRGSARPKLGANINLCCFYFVGMPVAVWLSFFSGFDFKGLWLGLFAAQGS 449

Query: 460 CAIAMLMALVRTNWEEQAERAKELTGSGSDDDDE 493
           C I+ML+ L RT+WE +  RAKEL     D D++
Sbjct: 450 CLISMLVVLARTDWEVEVHRAKELMTRSCDGDED 483

BLAST of Cp4.1LG13g01730 vs. TAIR 10
Match: AT1G58340.1 (MATE efflux family protein )

HSP 1 Score: 551.6 bits (1420), Expect = 6.5e-157
Identity = 289/492 (58.74%), Postives = 363/492 (73.78%), Query Frame = 0

Query: 2   SCSGSVLS--DQPKIPTNATTSLNAPLISE--------TERFPNLCVVLTELKCIADIAL 61
           S S S+LS  D+  I    T   + P  SE         +R+P+    L E+K I  I+ 
Sbjct: 8   SASSSLLSCKDKTHISKLETCDTDNPHYSEFRDTDSLDLKRWPSFLEGLEEVKAIGKISG 67

Query: 62  PMILVGFLMYSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQA 121
           P  + G LMYSR+MISMLFLG LG L LAGGSL+IGFANITGYSV+SGL++GMEPICGQA
Sbjct: 68  PTAMTGLLMYSRAMISMLFLGYLGELELAGGSLSIGFANITGYSVISGLSMGMEPICGQA 127

Query: 122 FGAKKFKLLGLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDISSEAHSYILCSVP 181
           +GAK+ KLLGL LQRTV+LLL  S+PISF W NM++ILL CGQ+++ISS A  ++L ++P
Sbjct: 128 YGAKQMKLLGLTLQRTVLLLLSCSVPISFSWLNMRRILLWCGQDEEISSVAQQFLLFAIP 187

Query: 182 DLIALSFLHPLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWT 241
           DL  LS LHPLRIYLR+Q+I LP+TY   ++++ H+P+NYLLV   ++G+ GVA+  V T
Sbjct: 188 DLFLLSLLHPLRIYLRTQNITLPVTYSTAVSVLLHVPLNYLLVVKLEMGVAGVAIAMVLT 247

Query: 242 NFNLVGLLILFIVLSGVYEKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILL 301
           N NLV LL  F+  + V+  TW  ++ + LK W +LL LAIP+C+SVCLEWWWYE MI+L
Sbjct: 248 NLNLVVLLSSFVYFTSVHSDTWVPITIDSLKGWSALLSLAIPTCVSVCLEWWWYEFMIIL 307

Query: 302 SGFMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLC 361
            G + NP++TVASMGILIQTTAL+Y+FPSSLS GVSTR+ NELGA RP KA+++ I+ L 
Sbjct: 308 CGLLANPRATVASMGILIQTTALVYVFPSSLSLGVSTRISNELGAKRPAKARVSMIISLF 367

Query: 362 TSFFLGLSALSFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRG 421
            +  LGL A+ FA  VR  W ++FT D  I++LT + LPI+GLCELGNCPQTT CGVLRG
Sbjct: 368 CAIALGLMAMVFAVLVRHHWGRLFTTDAEILQLTSIALPIVGLCELGNCPQTTGCGVLRG 427

Query: 422 TARPKLGANINLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTN 481
            ARP LGANINLG FY VGMP+AI   F     F GLW GLLAAQ +CA  ML AL+RT+
Sbjct: 428 CARPTLGANINLGSFYFVGMPVAILFGFVFKQGFPGLWFGLLAAQATCASLMLCALLRTD 487

Query: 482 WEEQAERAKELT 484
           W+ QAERA+ELT
Sbjct: 488 WKVQAERAEELT 499

BLAST of Cp4.1LG13g01730 vs. TAIR 10
Match: AT5G19700.1 (MATE efflux family protein )

HSP 1 Score: 509.2 bits (1310), Expect = 3.7e-144
Identity = 259/468 (55.34%), Postives = 339/468 (72.44%), Query Frame = 0

Query: 16  TNATTSLNAPLISETERFPNLCVVLTELKCIADIALPMILVGFLMYSRSMISMLFLGRLG 75
           TN  + ++    +    FP +  + +E + +  +A P IL   ++Y+RS ISMLFLG +G
Sbjct: 10  TNLLSKIDLEKQNPAPIFPTITELKSEARSLFSLAFPTILAALILYARSAISMLFLGHIG 69

Query: 76  GLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFKLLGLALQRTVILLLFSS 135
            L LAGGSLAI FANITGYSVL+GLA+GM+P+C QAFGA + KLL L LQRTV+ LL SS
Sbjct: 70  ELELAGGSLAIAFANITGYSVLAGLALGMDPLCSQAFGAGRPKLLSLTLQRTVLFLLTSS 129

Query: 136 IPISFLWFNMKKILLLCGQNDDISSEAHSYILCSVPDLIALSFLHPLRIYLRSQSINLPL 195
           + I  LW N+ KI++   Q+  ISS A +YILCS+PDL+  SFLHPLRIYLR+Q I  PL
Sbjct: 130 VVIVALWLNLGKIMIYLHQDPSISSLAQTYILCSIPDLLTNSFLHPLRIYLRAQGITSPL 189

Query: 196 TYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGLLILFIVLSGVYEKTWPG 255
           T       IFHIP+N+ LVS    G  GV++ A  +N  +V  L+  + ++G+++ TW  
Sbjct: 190 TLATLAGTIFHIPMNFFLVSYLGWGFMGVSMAAAASNLLVVIFLVAHVWIAGLHQPTWTR 249

Query: 256 MSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNPQSTVASMGILIQTTALI 315
            SSEC K+W  ++ LAIPSCI VCLEWWWYEIM +L G +++P + VASMGILIQTT+L+
Sbjct: 250 PSSECFKDWGPVVTLAIPSCIGVCLEWWWYEIMTVLCGLLIDPSTPVASMGILIQTTSLL 309

Query: 316 YIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSALSFAFNVRKVWAKMF 375
           YIFPSSL   VSTRVGNELG+NRPNKA+L+AIV +  +  +GL+A +FA+ V  VW  +F
Sbjct: 310 YIFPSSLGLAVSTRVGNELGSNRPNKARLSAIVAVSFAGVMGLTASAFAWGVSDVWGWIF 369

Query: 376 TNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGANINLGCFYMVGMPIAI 435
           TND  II+LT   LPI+GLCELGNCPQT  CGV+RGTARP + ANINLG FY+VG P+A+
Sbjct: 370 TNDVAIIKLTAAALPILGLCELGNCPQTVGCGVVRGTARPSMAANINLGAFYLVGTPVAV 429

Query: 436 WLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAERAKELT 484
            L+F+  + F GLW+GLLAAQ+ CA  ML  +  T+WE++A RA++LT
Sbjct: 430 GLTFWAAYGFCGLWVGLLAAQICCAAMMLYVVATTDWEKEAIRARKLT 477

BLAST of Cp4.1LG13g01730 vs. TAIR 10
Match: AT5G52050.1 (MATE efflux family protein )

HSP 1 Score: 504.2 bits (1297), Expect = 1.2e-142
Identity = 267/485 (55.05%), Postives = 349/485 (71.96%), Query Frame = 0

Query: 25  PLISETERFPN----LCVVLTELKCIADIALPMILVGFLMYSRSMISMLFLGRLGGLSLA 84
           PL+ +T    N    L V L E   I  I+ P++L G  +Y RS +S+ FLG LG  +LA
Sbjct: 14  PLLQKTSHLKNHSSVLSVFLNEAISICKISYPLVLTGLFLYVRSFVSLSFLGGLGDATLA 73

Query: 85  GGSLAIGFANITGYSVLSGLAVGMEPICGQAFGAKKFKLLGLALQRTVILLLFSSIPISF 144
           GGSLA  FANITGYS+ SGL +G+E IC QAFGA+++  +  +++R +ILLL +S+P++ 
Sbjct: 74  GGSLAAAFANITGYSLFSGLTMGVESICSQAFGARRYNYVCASVKRGIILLLVTSLPVTL 133

Query: 145 LWFNMKKILLLCGQNDDISSEAHSYILCSVPDLIALSFLHPLRIYLRSQSINLPLTYCAG 204
           LW NM+KILL+  Q+  ++SEAH ++L SVPDL+A SFLHPLR+YLR+QS  LPL+ C  
Sbjct: 134 LWMNMEKILLILKQDKKLASEAHIFLLYSVPDLVAQSFLHPLRVYLRTQSKTLPLSICTV 193

Query: 205 LAIIFHIPINYLLVSVFDLGIYGVALGAVWTNFNLVGLLILFI------VLSGVYEKTWP 264
           +A   H+PI + LVS   LGI G+AL  V +NFNLV  L L+I      +     EK   
Sbjct: 194 IASFLHLPITFFLVSYLGLGIKGIALSGVVSNFNLVAFLFLYICFFEDKLSVNEDEKITE 253

Query: 265 GMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGFMMNPQSTVASMGILIQTTAL 324
               + ++EWK LL LAIPSCISVCLEWW YEIMILL GF+++P+++VASMGILIQ T+L
Sbjct: 254 ETCEDSVREWKKLLCLAIPSCISVCLEWWCYEIMILLCGFLLDPKASVASMGILIQITSL 313

Query: 325 IYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSFFLGLSALSFAFNVRKVWAKM 384
           +YIFP SLS GVSTRVGNELG+N+P +A+ AAIVGL  S  LG +A +F  +VR  WA  
Sbjct: 314 VYIFPHSLSLGVSTRVGNELGSNQPKRARRAAIVGLGLSIALGFTAFAFTVSVRNTWAMF 373

Query: 385 FTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTARPKLGANINLGCFYMVGMPIA 444
           FT+D  I++LT + LPI+GLCELGNCPQTT CGVLRG+ARPK+GANIN   FY VG+P+ 
Sbjct: 374 FTDDKEIMKLTAMALPIVGLCELGNCPQTTGCGVLRGSARPKIGANINGVAFYAVGIPVG 433

Query: 445 IWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWEEQAERAKELTGS---GSDDDD 497
             L+F+ G+ FKGLW+G+LAAQ++C I M+ A  RT+WE +AERAK LT +   GS DDD
Sbjct: 434 AVLAFWFGFGFKGLWLGMLAAQITCVIGMMAATCRTDWELEAERAKVLTTAVDCGSSDDD 493

BLAST of Cp4.1LG13g01730 vs. TAIR 10
Match: AT4G29140.1 (MATE efflux family protein )

HSP 1 Score: 493.0 bits (1268), Expect = 2.7e-139
Identity = 246/463 (53.13%), Postives = 330/463 (71.27%), Query Frame = 0

Query: 29  ETERFPNLCVVLTELKCIADIALPMILVGFLMYSRSMISMLFLGRLGGLSLAGGSLAIGF 88
           E    P +   +TE K +  +A P+ +   ++Y RS +SM FLG+LG L LA GSLAI F
Sbjct: 43  ENRGSPLMAEAVTEAKSLFTLAFPIAVTALVLYLRSAVSMFFLGQLGDLELAAGSLAIAF 102

Query: 89  ANITGYSVLSGLAVGMEPICGQAFGAKKFKLLGLALQRTVILLLFSSIPISFLWFNMKKI 148
           ANITGYSVLSGLA+GMEP+C QAFGA +FKLL L L RTV+ LL   +PIS LWFN+ KI
Sbjct: 103 ANITGYSVLSGLALGMEPLCSQAFGAHRFKLLSLTLHRTVVFLLVCCVPISVLWFNVGKI 162

Query: 149 LLLCGQNDDISSEAHSYILCSVPDLIALSFLHPLRIYLRSQSINLPLTYCAGLAIIFHIP 208
            +   Q+ DI+  A +Y++ S+PDL+  + LHP+RIYLR+Q I  P+T  +    +FH+P
Sbjct: 163 SVYLHQDPDIAKLAQTYLIFSLPDLLTNTLLHPIRIYLRAQGIIHPVTLASLSGAVFHLP 222

Query: 209 INYLLVSVFDLGIYGVALGAVWTNFNLVGLLILFIVLSGVYEKTWPGMSSECLKEWKSLL 268
            N  LVS   LG+ GVA+ +  TN  +V  L+ ++  SG++  TW   + +C + W  LL
Sbjct: 223 ANLFLVSYLRLGLTGVAVASSITNIFVVAFLVCYVWASGLHAPTWTDPTRDCFRGWAPLL 282

Query: 269 ELAIPSCISVCLEWWWYEIMILLSGFMMNPQSTVASMGILIQTTALIYIFPSSLSFGVST 328
            LA PSC+SVCLEWWWYEIMI+L G ++NP+STVA+MG+LIQTT+ +Y+FPSSLSF VST
Sbjct: 283 RLAGPSCVSVCLEWWWYEIMIVLCGLLVNPRSTVAAMGVLIQTTSFLYVFPSSLSFAVST 342

Query: 329 RVGNELGANRPNKAKLAAIVGLCTSFFLGLSALSFAFNVRKVWAKMFTNDTGIIELTCLV 388
           RVGNELGANRP  AKL A V +  +   G+ A +FA++VR  W ++FT D  I++LT   
Sbjct: 343 RVGNELGANRPKTAKLTATVAIVFAAVTGIIAAAFAYSVRNAWGRIFTGDKEILQLTAAA 402

Query: 389 LPIIGLCELGNCPQTTSCGVLRGTARPKLGANINLGCFYMVGMPIAIWLSFYGGWDFKGL 448
           LPI+GLCE+GNCPQT  CGV+RGTARP   AN+NLG FY+VGMP+A+ L F+ G  F GL
Sbjct: 403 LPILGLCEIGNCPQTVGCGVVRGTARPSTAANVNLGAFYLVGMPVAVGLGFWAGIGFNGL 462

Query: 449 WIGLLAAQVSCAIAMLMALVRTNWEEQAERAKELTGSGSDDDD 492
           W+GLLAAQ+SCA  M+  +  T+WE +A++A+ LT + + ++D
Sbjct: 463 WVGLLAAQISCAGLMMYVVGTTDWESEAKKAQTLTCAETVEND 505

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O827522.2e-17869.82Protein DETOXIFICATION 49 OS=Arabidopsis thaliana OX=3702 GN=DTX49 PE=2 SV=1[more]
Q9SLV09.2e-15658.74Protein DETOXIFICATION 48 OS=Arabidopsis thaliana OX=3702 GN=DTX48 PE=2 SV=1[more]
Q4PSF45.2e-14355.34Protein DETOXIFICATION 52 OS=Arabidopsis thaliana OX=3702 GN=DTX52 PE=2 SV=1[more]
Q9FJ871.7e-14155.05Protein DETOXIFICATION 50 OS=Arabidopsis thaliana OX=3702 GN=DTX50 PE=2 SV=1[more]
Q9SZE23.9e-13853.13Protein DETOXIFICATION 51 OS=Arabidopsis thaliana OX=3702 GN=DTX51 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023551348.10.0100.00protein DETOXIFICATION 49-like [Cucurbita pepo subsp. pepo][more]
KAG7016948.10.098.80Protein DETOXIFICATION 49, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6579480.10.098.60Protein DETOXIFICATION 49, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022922331.10.098.00protein DETOXIFICATION 49-like [Cucurbita moschata][more]
XP_022970176.10.096.98protein DETOXIFICATION 49-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1E3V00.098.00Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111430342 PE=3 SV=1[more]
A0A6J1HZW90.096.98Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111469149 PE=3 SV=1[more]
A0A6J1H5E02.11e-28582.79Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111459742 PE=3 SV=1[more]
A0A0A0KKM44.04e-28483.85Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_5G155540 PE=3 SV=1[more]
A0A6J1K4X41.35e-28182.17Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111491221 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G23030.11.6e-17969.82MATE efflux family protein [more]
AT1G58340.16.5e-15758.74MATE efflux family protein [more]
AT5G19700.13.7e-14455.34MATE efflux family protein [more]
AT5G52050.11.2e-14255.05MATE efflux family protein [more]
AT4G29140.12.7e-13953.13MATE efflux family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002528Multi antimicrobial extrusion proteinPFAMPF01554MatEcoord: 273..435
e-value: 1.7E-32
score: 112.3
coord: 52..212
e-value: 3.6E-36
score: 124.3
IPR002528Multi antimicrobial extrusion proteinTIGRFAMTIGR00797TIGR00797coord: 52..449
e-value: 3.2E-62
score: 208.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 478..499
NoneNo IPR availablePANTHERPTHR11206:SF387PROTEIN DETOXIFICATIONcoord: 29..488
NoneNo IPR availablePANTHERPTHR11206MULTIDRUG RESISTANCE PROTEINcoord: 29..488
IPR045069Multidrug and toxic compound extrusion family, eukaryoticCDDcd13132MATE_eukaryoticcoord: 42..478
e-value: 7.76233E-173
score: 491.702

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG13g01730.1Cp4.1LG13g01730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015297 antiporter activity
molecular_function GO:0042910 xenobiotic transmembrane transporter activity