Cp4.1LG13g01190 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG13g01190
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionshort-chain dehydrogenase TIC 32, chloroplastic-like
LocationCp4.1LG13: 787034 .. 789745 (-)
RNA-Seq ExpressionCp4.1LG13g01190
SyntenyCp4.1LG13g01190
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCCATTCTATCAAGTACCTCGCCGGCTCCCCCGGCCCCTCCGGCTTCGGCTCCAAATCCTCCGCCGAACACGTCACCGCCTCCGCCCTCCACCTCCCTTCCATCACCGCCATCATCACCGGTAAGCTCAACTCCAACCTCCTCTCTTACTCTCATCGTTAAACTCTACTTAATCTCTTCTTCTTTCAGGCGCGACGTCGGGGATTGGAGCGGAGACGGCGCGGGTTCTTGCCAAACGCGGGGCTCGGTTAATCCTCCCGTCGCGGAACTTAAAGGCGGCCGAGGAAGCCAAGGCGCGGATCGAAGCTGAATGCTCTGGCTCGGAAATCATCGCCATGGCTCTGGATTTAAGCTCTCTGGCTTCCGTTAGAACCTTCGTTGCCCAATTTGAATCGCTGAATCTGCCGCTGAATCTCCTCATGTAAATCTCTGAAGAATCCATTGATTTCTGTTTTCCTCTGTCAATTGGGATTTTAATTTTTTAAAATTTTTCAGAAACAACGCCGGGAAATTCTCTCACGAGCACGCGATTTCGGAGGACGGGATCGAGATGACCTTCGCCACTAATTATTTGGGTAATTAAAAAAAAAATTAATTAAAATAATTATAATTAGAAATGGCGAATTAATGTTGTGAAATGTTTTCAGGTCATTTTTTATTGACGAAATTGTTGGTGAATAAGATGGTGGAGACGGCGGCGGCGACGGGGATTCAGGGGCGGATTGTGAACGTGTCCTCGAGCATTCACGGCTGGTTCTCCGGCGACATTATCAAATATCTCGGCCAGATAAGCCGAAATAAATGGTAAATTTAATATAAAAAAGGGTTTTCTTTTAATTTGTTTATTAATTTACAAATTTATCACACACAATTAAAATTTTGAAATTTTAGGACATCAAATTTTAATTTATTTAAAATTTAATATAAAAAGGGTTTTTAAAAAAAAAAAATAAAAAATTCTACGTTTATTGACTAAATTCTTTAAACCATCCATTTTGAAGTTTTTTTATGAATTCACAAATTGATGGACACAAAATAAAAAATAAAAAATTTAGGATATTAAATTCAAATTTATATATAACAAATTAGTTAATATAAAATTTTTCAGTTTTAAAATTTATTAAAAATGAATAAAAATGGTATTTAATTTAATATAATAATAGTATTTTTTTTTTTTGGGTAAAAAAAGATGGTGATTCTAAAACAGTTACTGTGGGTGAATTTCCAGCAGGAATTACGATGCGACACGTGCGTACGCGCTCTCTAAGCTTGCGAACGTTCTGCACACGCACGAACTTGCTCGCAGATTGAAGGTTCTTTACATTTATAATATTCCTTAAATACCCCACTCTTCCTTCTTTATTATAGGGTTTAATTCGTCTTTTTCCAACTCAGCTTTGACTCATATATTTTTTTTAATTTGCATTAATCATTATTATTGATTCTTTTCGTTATTAATTTTCTTAATGAAAAAGTAATTCTAAAAATAAATAAAAAAGGTTTTAATTTAAAATTTATTATACAAAATTAAAAATTATAATTCAAGAATTTGGTTAATTATTAATTTTTTATTATTTTAATTTAGAAAATGGGAGCTAATGTCACTGTGAATTGCGTTCATCCGGGAATCGTTAGAACTCGACTCACTAGAGATCGTGAAGGTTTCATTACAGGTACCAATTTTATTTAATTTTTTCTTTTATTACGAAGATTAAAATTAATTTATTTAATTTATGTTGAGGATGGTTGGGAGTGAGTCGGCTAATTTAGAGAATGATCATGAGTTTAAGTAAAAGAATACATTTGCATGAGTATGAGGCCTTTTGGAGAAGCCTAAAGCAAGTGGACAATATTATAGTTATTTCTAACCATTTATTTATTTATTTTGCAGATTTGGTGTTCTTTATAACTTCTAAACTGTTGAAGACAATCCCTCAGGTAATCAACCTAAACCTCTTAATACTTTTTATTCTTTCGCAGTCTTGTTGGGAGTGAGTCCCATAGCCATCCTGGAATTATAAATAAAGGAATACATCTCCATTAGCATGAGATCTTTTGGAGAAGTTCAAAGAAAAACCATGAGAGTTTATGCTCAAAGTGGACAATATCATATTATTGTGGAGAGTCGTGAGTTTTAACAGTTTTGATGTTGTTCTTCGTCTTCCACAGGCGGCGGCAACCACATGCTATGTGGCGGCAAATCCAAGACTAAGTAACATCACCGGGAAATACTTCGCCGACTGCAACGAAGCCTCGCCGTCGAAAATTGCCGCTAGTCTGACGGAAGCCGCCCGTCTCTGGTCGGCGTCGGAGATCATGGTCAACACAAACTCAAAACTCGTATTTAATCCAAACAATGCCTTGGACTAATTTAGTTTATTTAAAAAAAAAAAAAAAGAATTAAATACCTGAAAAAATAAATATAAATATATATTGTTTTGTTAGGAGAGAGAATGTTAGTCGAGAAATGAAATATAAAATATAATGGTAGATGGTAGGTAGACAAAAAGCAGGAAGAAGGTAGGTAGGTAGTGGAGGGAATGAATATATTTAGGTTTACAAATATAATATATAGTTTTTAGCTTTATTTTGTGTATAATATATAGTTTCTTTTAGGTTTTAATTTAATGTTGGTTGTGACATATAATAAGCCAAGTGCTCTCTTTATACTTATATTTAATCATTGGACCCATCAAGTATTGGAGGATT

mRNA sequence

ATGCTCCATTCTATCAAGTACCTCGCCGGCTCCCCCGGCCCCTCCGGCTTCGGCTCCAAATCCTCCGCCGAACACGTCACCGCCTCCGCCCTCCACCTCCCTTCCATCACCGCCATCATCACCGGCGCGACGTCGGGGATTGGAGCGGAGACGGCGCGGGTTCTTGCCAAACGCGGGGCTCGGTTAATCCTCCCGTCGCGGAACTTAAAGGCGGCCGAGGAAGCCAAGGCGCGGATCGAAGCTGAATGCTCTGGCTCGGAAATCATCGCCATGGCTCTGGATTTAAGCTCTCTGGCTTCCGTTAGAACCTTCGTTGCCCAATTTGAATCGCTGAATCTGCCGCTGAATCTCCTCATAAACAACGCCGGGAAATTCTCTCACGAGCACGCGATTTCGGAGGACGGGATCGAGATGACCTTCGCCACTAATTATTTGGGTCATTTTTTATTGACGAAATTGTTGGTGAATAAGATGGTGGAGACGGCGGCGGCGACGGGGATTCAGGGGCGGATTGTGAACGTGTCCTCGAGCATTCACGGCTGGTTCTCCGGCGACATTATCAAATATCTCGGCCAGATAAGCCGAAATAAATGCAGGAATTACGATGCGACACGTGCGTACGCGCTCTCTAAGCTTGCGAACGTTCTGCACACGCACGAACTTGCTCGCAGATTGAAGAAAATGGGAGCTAATGTCACTGTGAATTGCGTTCATCCGGGAATCGTTAGAACTCGACTCACTAGAGATCGTGAAGGTTTCATTACAGATTTGGTGTTCTTTATAACTTCTAAACTGTTGAAGACAATCCCTCAGGCGGCGGCAACCACATGCTATGTGGCGGCAAATCCAAGACTAAGTAACATCACCGGGAAATACTTCGCCGACTGCAACGAAGCCTCGCCGTCGAAAATTGCCGCTAGTCTGACGGAAGCCGCCCGTCTCTGGTCGGCGTCGGAGATCATGGTCAACACAAACTCAAAACTCGTATTTAATCCAAACAATGCCTTGGACTAATTTAGTTTATTTAAAAAAAAAAAAAAAGAATTAAATACCTGAAAAAATAAATATAAATATATATTGTTTTGTTAGGAGAGAGAATGTTAGTCGAGAAATGAAATATAAAATATAATGGTAGATGGTAGGTAGACAAAAAGCAGGAAGAAGGTAGGTAGGTAGTGGAGGGAATGAATATATTTAGGTTTACAAATATAATATATAGTTTTTAGCTTTATTTTGTGTATAATATATAGTTTCTTTTAGGTTTTAATTTAATGTTGGTTGTGACATATAATAAGCCAAGTGCTCTCTTTATACTTATATTTAATCATTGGACCCATCAAGTATTGGAGGATT

Coding sequence (CDS)

ATGCTCCATTCTATCAAGTACCTCGCCGGCTCCCCCGGCCCCTCCGGCTTCGGCTCCAAATCCTCCGCCGAACACGTCACCGCCTCCGCCCTCCACCTCCCTTCCATCACCGCCATCATCACCGGCGCGACGTCGGGGATTGGAGCGGAGACGGCGCGGGTTCTTGCCAAACGCGGGGCTCGGTTAATCCTCCCGTCGCGGAACTTAAAGGCGGCCGAGGAAGCCAAGGCGCGGATCGAAGCTGAATGCTCTGGCTCGGAAATCATCGCCATGGCTCTGGATTTAAGCTCTCTGGCTTCCGTTAGAACCTTCGTTGCCCAATTTGAATCGCTGAATCTGCCGCTGAATCTCCTCATAAACAACGCCGGGAAATTCTCTCACGAGCACGCGATTTCGGAGGACGGGATCGAGATGACCTTCGCCACTAATTATTTGGGTCATTTTTTATTGACGAAATTGTTGGTGAATAAGATGGTGGAGACGGCGGCGGCGACGGGGATTCAGGGGCGGATTGTGAACGTGTCCTCGAGCATTCACGGCTGGTTCTCCGGCGACATTATCAAATATCTCGGCCAGATAAGCCGAAATAAATGCAGGAATTACGATGCGACACGTGCGTACGCGCTCTCTAAGCTTGCGAACGTTCTGCACACGCACGAACTTGCTCGCAGATTGAAGAAAATGGGAGCTAATGTCACTGTGAATTGCGTTCATCCGGGAATCGTTAGAACTCGACTCACTAGAGATCGTGAAGGTTTCATTACAGATTTGGTGTTCTTTATAACTTCTAAACTGTTGAAGACAATCCCTCAGGCGGCGGCAACCACATGCTATGTGGCGGCAAATCCAAGACTAAGTAACATCACCGGGAAATACTTCGCCGACTGCAACGAAGCCTCGCCGTCGAAAATTGCCGCTAGTCTGACGGAAGCCGCCCGTCTCTGGTCGGCGTCGGAGATCATGGTCAACACAAACTCAAAACTCGTATTTAATCCAAACAATGCCTTGGACTAA

Protein sequence

MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGARLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLINNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAAATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNITGKYFADCNEASPSKIAASLTEAARLWSASEIMVNTNSKLVFNPNNALD
Homology
[Close]
BLAST of Cp4.1LG13g01190 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 1.5e-69
Identity = 155/319 (48.59%), Postives = 207/319 (64.89%), Query Frame = 0 

Query: 8   LAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGARLILPSR 67
           + G  G SG+GS S+AE VT S +    +TAIITG TSGIG E ARVL  RGA +I+ SR
Sbjct: 7   VTGKAGKSGYGSASTAEDVTHS-IDAKHLTAIITGGTSGIGLEAARVLGMRGAHVIIASR 66

Query: 68  NLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLINNAGKFSH 127
           N KAA ++K  I      + I  + LDLSS+ SVR+F+ QF +LN+PLN+LINNAG    
Sbjct: 67  NTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVMFC 126

Query: 128 EHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAAATGIQGRIVNVSSSIH--GWFSGD 187
              +SEDGIE  FATN++GHFLLT LL++KM  +A  +GI+GRIVN+SS  H   +  G 
Sbjct: 127 PFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTEGI 186

Query: 188 IIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPGIVRTR 247
           +  Y+    R     Y   +AY  SKLAN+LH++ L+R+L++ G N+T+N VHPG++ T 
Sbjct: 187 MFDYINDPDR-----YSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTN 246

Query: 248 LTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNITGKYFADCNEASPSKIA 307
           L R   G    ++  ++  L K IPQ AATTCYVA +P L ++TGKYFADCN  +PS  A
Sbjct: 247 LFR-HSGLGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKDVTGKYFADCNVTTPSNFA 306

Query: 308 ASLTEAARLWSASEIMVNT 325
              T A +LW  S  +V +
Sbjct: 307 IDTTLADKLWDFSIKLVES 318

[Close]
BLAST of Cp4.1LG13g01190 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 9.8e-69
Identity = 153/319 (47.96%), Postives = 206/319 (64.58%), Query Frame = 0 

Query: 8   LAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGARLILPSR 67
           + G  G SG+GS S+AE VT S +    +TAIITG TSGIG E ARVL  RGA +I+ +R
Sbjct: 7   VTGKAGKSGYGSASTAEDVTHS-IDAKHLTAIITGGTSGIGLEAARVLGMRGAHVIIAAR 66

Query: 68  NLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLINNAGKFSH 127
           N KAA ++K  I      + I  + LDLSS+ SVR+F+ QF +LN+PLN+LINNAG    
Sbjct: 67  NTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVMFC 126

Query: 128 EHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAAATGIQGRIVNVSSSIH--GWFSGD 187
              +SEDGIE  FATN++GHFLLT LL++KM  +A  +GI+GRIVN+SS  H   +  G 
Sbjct: 127 PFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTEGI 186

Query: 188 IIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPGIVRTR 247
           +  Y+    R     Y   +AY  SKLAN+LH++ L+R+L++ G N+T+N VHPG++ T 
Sbjct: 187 MFDYINDPDR-----YSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTN 246

Query: 248 LTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNITGKYFADCNEASPSKIA 307
           L R   G    ++  ++  L K IPQ AATTCYVA +P L  +TGKYF DCN  +PS  A
Sbjct: 247 LFR-HSGLGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKGVTGKYFTDCNVTTPSNFA 306

Query: 308 ASLTEAARLWSASEIMVNT 325
              T A +LW  S  +V++
Sbjct: 307 IDTTLADKLWDFSIKLVDS 318

[Close]
BLAST of Cp4.1LG13g01190 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 2.1e-63
Identity = 137/317 (43.22%), Postives = 201/317 (63.41%), Query Frame = 0 

Query: 10  GSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGARLILPSRNL 69
           GS G SGF S+S+AE VT   +    +TAI+TGA+SGIG ETARVL+ RG  +++  RN 
Sbjct: 5   GSKGASGFSSRSTAEEVT-HGVDGTGLTAIVTGASSGIGVETARVLSLRGVHVVMAVRNT 64

Query: 70  KAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLINNAGKFSHEH 129
            +  + K  I  +  G+++  M LDLSS+ SVR F ++++S  LPLNLLINNAG  +   
Sbjct: 65  DSGAKVKEDIVKQVPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMACPF 124

Query: 130 AISEDGIEMTFATNYLGHFLLTKLLVNKMVETAAATGIQGRIVNVSSSIHGWFSGDIIKY 189
            +S+D IE+ FATN+LGHFLLTKLL++ M  T+  +  +GRIVN+SS  H +   + +++
Sbjct: 125 MLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEGVRF 184

Query: 190 LGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPGIVRTRLTRD 249
                 N   +Y + RAY  SKL NVLH +EL ++LK+ G N+T N +HPG + T L R 
Sbjct: 185 ---DKINDKSSYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIMTNLGRY 244

Query: 250 REGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNITGKYFADCNEASPSKIAASLT 309
              ++   V  +   +LK++PQ AATTCYVA NP+++ ++G+YF D N A P  +     
Sbjct: 245 FNPYLAVAVGAVAKYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDTE 304

Query: 310 EAARLWSASEIMVNTNS 327
            A ++W  S  + ++ S
Sbjct: 305 LAKKVWDFSTKLTDSQS 317

[Close]
BLAST of Cp4.1LG13g01190 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 3.5e-58
Identity = 133/306 (43.46%), Postives = 191/306 (62.42%), Query Frame = 0 

Query: 13  GPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGARLILPSRNLKAA 72
           G SGF   S+AE VT   +    +TAI+TGA+SGIGAET RVLA RGA +I+  RN+ AA
Sbjct: 9   GVSGFSGSSTAEQVT-HGIDATGLTAIVTGASSGIGAETTRVLALRGAHVIMGVRNMVAA 68

Query: 73  EEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLINNAGKFSHEHAIS 132
           ++ K  I  +   +++ A+ LDLSSL SV+ F ++F S   PLN+LINNAG  +    +S
Sbjct: 69  KDVKDTILKDIPSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIMACPFKLS 128

Query: 133 EDGIEMTFATNYLGHFLLTKLLVNKMVETAAATGIQGRIVNVSSSIHGWFSGDIIKYLGQ 192
           +D IE+ FATN++GHFLLT LL++ M +T   +  +GRIVNV+S  H +   + I++   
Sbjct: 129 KDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPEGIRF--- 188

Query: 193 ISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPGIVRTRLTRDREG 252
              N   +Y+  RAY  SKLANVLH ++L + LK+ G N+T N +HPG + T L R    
Sbjct: 189 DKINDQSSYNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFRHNSA 248

Query: 253 FITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNITGKYFADCNEASPSKIAASLTEAA 312
            +  L+  I   +LK + Q AATTCYVA +P++  ++G+YF+D N    +     +  A 
Sbjct: 249 -VNGLINVIGKLVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVYKTTPHGKDVDLAK 308

Query: 313 RLWSAS 319
           +LW  S
Sbjct: 309 KLWDFS 309

[Close]
BLAST of Cp4.1LG13g01190 vs. ExPASy Swiss-Prot
Match: P59837 (Retinol dehydrogenase 12 OS=Bos taurus OX=9913 GN=RDH12 PE=2 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 2.1e-42
Identity = 116/283 (40.99%), Postives = 164/283 (57.95%), Query Frame = 0 

Query: 39  IITGATSGIGAETARVLAKRGARLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSL 98
           +ITGA +GIG ETAR LA+RGAR+ +  R++   E A + I+A+   S+++   LDLS  
Sbjct: 43  VITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIQADTKNSQVLVRKLDLSDT 102

Query: 99  ASVRTFVAQFESLNLPLNLLINNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKM 158
            S+R F   F +    L++LINNAG     ++ + DG E   A N+LGHFLLT LL+ ++
Sbjct: 103 KSIRAFAEGFLAEEKQLHILINNAGVMLCPYSKTADGFETHLAVNHLGHFLLTHLLLGRL 162

Query: 159 VETAAATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRN---YDATRAYALSKLANV 218
            E+A A     R+VN+SS  H         +LG+I  +  +    Y+   AY  SKLANV
Sbjct: 163 KESAPA-----RVVNLSSVAH---------HLGKIRFHDLQGDKYYNLGFAYCHSKLANV 222

Query: 219 LHTHELARRLKKMGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIPQAAAT 278
           L T ELA+RLK  G  VT   VHPGIVR++L   R  F+  L++ + S  LKT  + A T
Sbjct: 223 LFTRELAKRLK--GTGVTTYAVHPGIVRSKLV--RHSFLLCLLWRLFSPFLKTTWEGAQT 282

Query: 279 TCYVAANPRLSNITGKYFADCNEASPSKIAASLTEAARLWSAS 319
           + + A    L  ++GKYF+DC +   S  A +   A RLW+ S
Sbjct: 283 SLHCALAEGLEPLSGKYFSDCKKTWVSPRARNNKTAERLWNVS 307

[Close]
BLAST of Cp4.1LG13g01190 vs. NCBI nr
Match: XP_023550055.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 647 bits (1668), Expect = 1.38e-233
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0 

Query: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA 60
           MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA
Sbjct: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA 60

Query: 61  RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLIN 120
           RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLIN
Sbjct: 61  RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLIN 120

Query: 121 NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAAATGIQGRIVNVSSSIHG 180
           NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAAATGIQGRIVNVSSSIHG
Sbjct: 121 NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAAATGIQGRIVNVSSSIHG 180

Query: 181 WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPG 240
           WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPG
Sbjct: 181 WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPG 240

Query: 241 IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNITGKYFADCNEAS 300
           IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNITGKYFADCNEAS
Sbjct: 241 IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNITGKYFADCNEAS 300

Query: 301 PSKIAASLTEAARLWSASEIMVNTNSKLVFNPNNALD 337
           PSKIAASLTEAARLWSASEIMVNTNSKLVFNPNNALD
Sbjct: 301 PSKIAASLTEAARLWSASEIMVNTNSKLVFNPNNALD 337

[Close]
BLAST of Cp4.1LG13g01190 vs. NCBI nr
Match: XP_022970067.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 640 bits (1650), Expect = 7.65e-231
Identity = 332/337 (98.52%), Postives = 335/337 (99.41%), Query Frame = 0 

Query: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA 60
           MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA
Sbjct: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA 60

Query: 61  RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLIN 120
           RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLIN
Sbjct: 61  RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLIN 120

Query: 121 NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAAATGIQGRIVNVSSSIHG 180
           NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVN MVETA ATGIQGRIVNVSSSIHG
Sbjct: 121 NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNTMVETAKATGIQGRIVNVSSSIHG 180

Query: 181 WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPG 240
           WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPG
Sbjct: 181 WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPG 240

Query: 241 IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNITGKYFADCNEAS 300
           IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSN+TGKYFADCNEAS
Sbjct: 241 IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNVTGKYFADCNEAS 300

Query: 301 PSKIAASLTEAARLWSASEIMVNTNSKLVFNPNNALD 337
           PSK+AASLTEAARLWSASEIMVNTNSKLVFNPNNAL+
Sbjct: 301 PSKLAASLTEAARLWSASEIMVNTNSKLVFNPNNALE 337

[Close]
BLAST of Cp4.1LG13g01190 vs. NCBI nr
Match: XP_022929143.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 637 bits (1642), Expect = 1.27e-229
Identity = 329/337 (97.63%), Postives = 333/337 (98.81%), Query Frame = 0 

Query: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA 60
           MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA
Sbjct: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA 60

Query: 61  RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLIN 120
           RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLL+N
Sbjct: 61  RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLVN 120

Query: 121 NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAAATGIQGRIVNVSSSIHG 180
           NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVN MVET  ATGIQGRIVNVSSSIHG
Sbjct: 121 NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNTMVETTKATGIQGRIVNVSSSIHG 180

Query: 181 WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPG 240
           WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKK GAN+TVNCVHPG
Sbjct: 181 WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKTGANITVNCVHPG 240

Query: 241 IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNITGKYFADCNEAS 300
           IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSN+TGKYFADCNEAS
Sbjct: 241 IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNVTGKYFADCNEAS 300

Query: 301 PSKIAASLTEAARLWSASEIMVNTNSKLVFNPNNALD 337
           PSK+AASLTEAARLWSASEIMVNTNSKLVFNPNNALD
Sbjct: 301 PSKLAASLTEAARLWSASEIMVNTNSKLVFNPNNALD 337

[Close]
BLAST of Cp4.1LG13g01190 vs. NCBI nr
Match: KAG6579556.1 (Short-chain dehydrogenase TIC 32 A, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 634 bits (1636), Expect = 1.04e-228
Identity = 329/337 (97.63%), Postives = 333/337 (98.81%), Query Frame = 0 

Query: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA 60
           MLHSIKYLAGSPGPSGFGSKSSAEHV+ASALHLPSITAIITGATSGIGAETARVLAKRGA
Sbjct: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVSASALHLPSITAIITGATSGIGAETARVLAKRGA 60

Query: 61  RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLIN 120
           RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLL+N
Sbjct: 61  RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLVN 120

Query: 121 NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAAATGIQGRIVNVSSSIHG 180
           NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVN MVETA ATGIQGRIVNVSSSIHG
Sbjct: 121 NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNTMVETAKATGIQGRIVNVSSSIHG 180

Query: 181 WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPG 240
           WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKK GANVTVNCVHPG
Sbjct: 181 WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKTGANVTVNCVHPG 240

Query: 241 IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNITGKYFADCNEAS 300
           IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSN+TGKYFADCNEAS
Sbjct: 241 IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNVTGKYFADCNEAS 300

Query: 301 PSKIAASLTEAARLWSASEIMVNTNSKLVFNPNNALD 337
           PSK+AAS TEAARLWSASEIMVNTNSKLVFNPNNALD
Sbjct: 301 PSKLAASPTEAARLWSASEIMVNTNSKLVFNPNNALD 337

[Close]
BLAST of Cp4.1LG13g01190 vs. NCBI nr
Match: XP_022970068.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 629 bits (1622), Expect = 1.36e-226
Identity = 330/337 (97.92%), Postives = 333/337 (98.81%), Query Frame = 0 

Query: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA 60
           MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA
Sbjct: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA 60

Query: 61  RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLIN 120
           RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLIN
Sbjct: 61  RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLIN 120

Query: 121 NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAAATGIQGRIVNVSSSIHG 180
           NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVN MVETA ATGIQGRIVNVSSSIHG
Sbjct: 121 NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNTMVETAKATGIQGRIVNVSSSIHG 180

Query: 181 WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPG 240
           WFSGDIIKYLGQISRNK  NYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPG
Sbjct: 181 WFSGDIIKYLGQISRNKW-NYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPG 240

Query: 241 IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNITGKYFADCNEAS 300
           IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSN+TGKYFADCNEAS
Sbjct: 241 IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNVTGKYFADCNEAS 300

Query: 301 PSKIAASLTEAARLWSASEIMVNTNSKLVFNPNNALD 337
           PSK+AASLTEAARLWSASEIMVNTNSKLVFNPNNAL+
Sbjct: 301 PSKLAASLTEAARLWSASEIMVNTNSKLVFNPNNALE 336

[Close]
BLAST of Cp4.1LG13g01190 vs. ExPASy TrEMBL
Match: A0A6J1HY34 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111469067 PE=3 SV=1)

HSP 1 Score: 640 bits (1650), Expect = 3.70e-231
Identity = 332/337 (98.52%), Postives = 335/337 (99.41%), Query Frame = 0 

Query: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA 60
           MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA
Sbjct: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA 60

Query: 61  RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLIN 120
           RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLIN
Sbjct: 61  RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLIN 120

Query: 121 NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAAATGIQGRIVNVSSSIHG 180
           NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVN MVETA ATGIQGRIVNVSSSIHG
Sbjct: 121 NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNTMVETAKATGIQGRIVNVSSSIHG 180

Query: 181 WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPG 240
           WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPG
Sbjct: 181 WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPG 240

Query: 241 IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNITGKYFADCNEAS 300
           IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSN+TGKYFADCNEAS
Sbjct: 241 IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNVTGKYFADCNEAS 300

Query: 301 PSKIAASLTEAARLWSASEIMVNTNSKLVFNPNNALD 337
           PSK+AASLTEAARLWSASEIMVNTNSKLVFNPNNAL+
Sbjct: 301 PSKLAASLTEAARLWSASEIMVNTNSKLVFNPNNALE 337

[Close]
BLAST of Cp4.1LG13g01190 vs. ExPASy TrEMBL
Match: A0A6J1ER89 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435812 PE=3 SV=1)

HSP 1 Score: 637 bits (1642), Expect = 6.13e-230
Identity = 329/337 (97.63%), Postives = 333/337 (98.81%), Query Frame = 0 

Query: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA 60
           MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA
Sbjct: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA 60

Query: 61  RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLIN 120
           RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLL+N
Sbjct: 61  RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLVN 120

Query: 121 NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAAATGIQGRIVNVSSSIHG 180
           NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVN MVET  ATGIQGRIVNVSSSIHG
Sbjct: 121 NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNTMVETTKATGIQGRIVNVSSSIHG 180

Query: 181 WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPG 240
           WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKK GAN+TVNCVHPG
Sbjct: 181 WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKTGANITVNCVHPG 240

Query: 241 IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNITGKYFADCNEAS 300
           IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSN+TGKYFADCNEAS
Sbjct: 241 IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNVTGKYFADCNEAS 300

Query: 301 PSKIAASLTEAARLWSASEIMVNTNSKLVFNPNNALD 337
           PSK+AASLTEAARLWSASEIMVNTNSKLVFNPNNALD
Sbjct: 301 PSKLAASLTEAARLWSASEIMVNTNSKLVFNPNNALD 337

[Close]
BLAST of Cp4.1LG13g01190 vs. ExPASy TrEMBL
Match: A0A6J1I2S6 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111469067 PE=3 SV=1)

HSP 1 Score: 629 bits (1622), Expect = 6.60e-227
Identity = 330/337 (97.92%), Postives = 333/337 (98.81%), Query Frame = 0 

Query: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA 60
           MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA
Sbjct: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA 60

Query: 61  RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLIN 120
           RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLIN
Sbjct: 61  RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLIN 120

Query: 121 NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAAATGIQGRIVNVSSSIHG 180
           NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVN MVETA ATGIQGRIVNVSSSIHG
Sbjct: 121 NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNTMVETAKATGIQGRIVNVSSSIHG 180

Query: 181 WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPG 240
           WFSGDIIKYLGQISRNK  NYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPG
Sbjct: 181 WFSGDIIKYLGQISRNKW-NYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPG 240

Query: 241 IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNITGKYFADCNEAS 300
           IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSN+TGKYFADCNEAS
Sbjct: 241 IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNVTGKYFADCNEAS 300

Query: 301 PSKIAASLTEAARLWSASEIMVNTNSKLVFNPNNALD 337
           PSK+AASLTEAARLWSASEIMVNTNSKLVFNPNNAL+
Sbjct: 301 PSKLAASLTEAARLWSASEIMVNTNSKLVFNPNNALE 336

[Close]
BLAST of Cp4.1LG13g01190 vs. ExPASy TrEMBL
Match: A0A6J1EMX3 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111435812 PE=3 SV=1)

HSP 1 Score: 626 bits (1614), Expect = 1.09e-225
Identity = 327/337 (97.03%), Postives = 331/337 (98.22%), Query Frame = 0 

Query: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA 60
           MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA
Sbjct: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA 60

Query: 61  RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLIN 120
           RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLL+N
Sbjct: 61  RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLVN 120

Query: 121 NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAAATGIQGRIVNVSSSIHG 180
           NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVN MVET  ATGIQGRIVNVSSSIHG
Sbjct: 121 NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNTMVETTKATGIQGRIVNVSSSIHG 180

Query: 181 WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPG 240
           WFSGDIIKYLGQISRNK  NYDATRAYALSKLANVLHTHELARRLKK GAN+TVNCVHPG
Sbjct: 181 WFSGDIIKYLGQISRNKW-NYDATRAYALSKLANVLHTHELARRLKKTGANITVNCVHPG 240

Query: 241 IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNITGKYFADCNEAS 300
           IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSN+TGKYFADCNEAS
Sbjct: 241 IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNVTGKYFADCNEAS 300

Query: 301 PSKIAASLTEAARLWSASEIMVNTNSKLVFNPNNALD 337
           PSK+AASLTEAARLWSASEIMVNTNSKLVFNPNNALD
Sbjct: 301 PSKLAASLTEAARLWSASEIMVNTNSKLVFNPNNALD 336

[Close]
BLAST of Cp4.1LG13g01190 vs. ExPASy TrEMBL
Match: A0A0A0KKE2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G149870 PE=3 SV=1)

HSP 1 Score: 571 bits (1471), Expect = 6.47e-204
Identity = 293/337 (86.94%), Postives = 315/337 (93.47%), Query Frame = 0 

Query: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA 60
           ML S  YL+GSPGPSGFGSKS+AEHVTAS    PS TAIITGATSGIGAETAR+LAKRGA
Sbjct: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTASLP--PSFTAIITGATSGIGAETARILAKRGA 60

Query: 61  RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLIN 120
           RLILPSRN+KAAEE KARIE+ECS SEII M+LDLSSL+SVRTFV+QFESLNLPLNLLIN
Sbjct: 61  RLILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLLIN 120

Query: 121 NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAAATGIQGRIVNVSSSIHG 180
           NAGKFSHEHAI+EDGIEMTFATNYLGHFLLTKLLVNKMVETA  TG++GRIVNVSSSIHG
Sbjct: 121 NAGKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSIHG 180

Query: 181 WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPG 240
           WFSGDI+KYLGQISRNK RNYDATRAYALSKLANVLHTHELARR K+MGANVTVNCVHPG
Sbjct: 181 WFSGDILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHPG 240

Query: 241 IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNITGKYFADCNEAS 300
           IVRTRLTRDREGFITDLVFF+ SKLLKTIPQAAATTCYVA NPRL ++TGKYF DCNE+S
Sbjct: 241 IVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNESS 300

Query: 301 PSKIAASLTEAARLWSASEIMVNTNSKLVFNPNNALD 337
           PSK+A S +EAARLWSASEIMVN NSKL+F+PNNAL+
Sbjct: 301 PSKLAGSPSEAARLWSASEIMVNANSKLLFDPNNALE 335

[Close]
BLAST of Cp4.1LG13g01190 vs. TAIR 10
Match: AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 469.5 bits (1207), Expect = 2.2e-132
Identity = 242/332 (72.89%), Postives = 285/332 (85.84%), Query Frame = 0 

Query: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA 60
           M+ + KYL G+ G SGFGSKS+AE VT +   L SITA+ITGATSGIGAETARVLAKRGA
Sbjct: 1   MIETGKYLLGAAGASGFGSKSTAEEVTENC-DLRSITAVITGATSGIGAETARVLAKRGA 60

Query: 61  RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLIN 120
           RLI P+RN+KAAEEAK RI +E   +EI+ M LDLSS+ASVR FVA FESL+LPLNLLIN
Sbjct: 61  RLIFPARNVKAAEEAKERIVSEFPETEIVVMKLDLSSIASVRNFVADFESLDLPLNLLIN 120

Query: 121 NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAAATGIQGRIVNVSSSIHG 180
           NAGK +HEHAISEDGIEMTFATNYLGHFLLT LL+NKM++TA  TG+QGRIVNV+S IHG
Sbjct: 121 NAGKLAHEHAISEDGIEMTFATNYLGHFLLTNLLLNKMIQTAEETGVQGRIVNVTSGIHG 180

Query: 181 WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPG 240
           WFSGD+I+YL  IS+ KC+ +DATRAYALSKLANVLHT EL+ RL+K+GANVTVNCVHPG
Sbjct: 181 WFSGDLIEYLRLISQPKCQ-FDATRAYALSKLANVLHTKELSSRLQKIGANVTVNCVHPG 240

Query: 241 IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNITGKYFADCNEAS 300
           +VRTRLTRDREG +TDLVFF+ SKL+KT+PQAAATTCYVA NPRL N++GKYF DCNE +
Sbjct: 241 VVRTRLTRDREGLLTDLVFFLASKLVKTVPQAAATTCYVATNPRLVNVSGKYFTDCNETT 300

Query: 301 PSKIAASLTEAARLWSASEIMVNTNSKLVFNP 333
           PS +  + +EA +LW+ASEI+V  +SK  F+P
Sbjct: 301 PSGLGTNSSEATKLWAASEILVTQHSKTSFDP 330

[Close]
BLAST of Cp4.1LG13g01190 vs. TAIR 10
Match: AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 455.7 bits (1171), Expect = 3.3e-128
Identity = 233/323 (72.14%), Postives = 279/323 (86.38%), Query Frame = 0 

Query: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGA 60
           M+ ++K+L GS GPSGFGS+S+A+HVT ++  L S+TAIITGATSGIGAETARVLAKRGA
Sbjct: 1   MIETVKHLIGSGGPSGFGSRSTADHVTCNS-DLRSLTAIITGATSGIGAETARVLAKRGA 60

Query: 61  RLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLIN 120
           RL+LP+R++K AEE KARI +E   +EII M LDLSSL SVR FV  FESLNLPLN+LIN
Sbjct: 61  RLVLPARSVKTAEETKARILSEFPDAEIIVMHLDLSSLTSVRRFVDDFESLNLPLNILIN 120

Query: 121 NAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAAATGIQGRIVNVSSSIHG 180
           NAGK++H+HA+SEDG+EMTFATNYLGHFLLTKLL+ KM+ETAA TG+QGRIVNV+S +H 
Sbjct: 121 NAGKYAHKHALSEDGVEMTFATNYLGHFLLTKLLLKKMIETAAQTGVQGRIVNVTSVVHS 180

Query: 181 WFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPG 240
           WFSGD+++YL  ISRN  RNYDATRAYALSKLANVLHT EL+R L KM ANVT NCVHPG
Sbjct: 181 WFSGDMLQYLADISRNN-RNYDATRAYALSKLANVLHTVELSRLLHKMDANVTANCVHPG 240

Query: 241 IVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNITGKYFADCNEAS 300
           IV+TRLTRDREG +TDLVFF+TSKLLK++PQAAATTCYVA +PRL N+ GKYF+DCNEA 
Sbjct: 241 IVKTRLTRDREGVVTDLVFFLTSKLLKSVPQAAATTCYVATSPRLRNVCGKYFSDCNEAR 300

Query: 301 PSKIAASLTEAARLWSASEIMVN 324
            SK  +   +A RLW+AS+++V+
Sbjct: 301 SSKSGSCNLKAQRLWTASDLLVS 321

[Close]
BLAST of Cp4.1LG13g01190 vs. TAIR 10
Match: AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 305.4 bits (781), Expect = 5.5e-83
Identity = 172/327 (52.60%), Postives = 225/327 (68.81%), Query Frame = 0 

Query: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTA-SALHLPSITAIITGATSGIGAETARVLAKRG 60
           M  +++YLAG  GP+GFGS+S+AE VT  S      +TAIITG TSGIGAETARVLAKRG
Sbjct: 1   MKGTLRYLAGIAGPNGFGSRSTAEQVTQHSFFPCSHLTAIITGGTSGIGAETARVLAKRG 60

Query: 61  ARLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLI 120
            R+++  R++K AE  K RI  E   ++II   +DLSSL+SV  F +QF S +LPLN+LI
Sbjct: 61  VRVVMAVRDMKKAEMVKERIIRENPEADIILFEIDLSSLSSVARFCSQFLSQDLPLNILI 120

Query: 121 NNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAAATGIQGRIVNVSSSIH 180
           NNAG FS     SE+ IE+TFATN+LGH+LLT++L+ KM++TA  +GI+GRI+N+SS IH
Sbjct: 121 NNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVIH 180

Query: 181 GWFSGDII---KYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNC 240
            W   D     K L  ISR     Y+ TRAYA SKLA +LH   L+++LK   ANVT+N 
Sbjct: 181 NWVKPDCFSFPKLLHPISR-----YNGTRAYAQSKLATILHAKALSKQLKDRNANVTINA 240

Query: 241 VHPGIVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNITGKYFADC 300
           VHPGIV+T + R  +G  TD +F I SKLLK+I Q AATTCYVA +     ++GKYFADC
Sbjct: 241 VHPGIVKTGIIRAHKGLFTDSLFLIASKLLKSISQGAATTCYVALSNETKGLSGKYFADC 300

Query: 301 NEASPSKIAASLTEAARLWSASEIMVN 324
           NE + S +A     A +L + S  +++
Sbjct: 301 NETNCSDLANDEYVALKLCTQSRALIH 322

[Close]
BLAST of Cp4.1LG13g01190 vs. TAIR 10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 285.8 bits (730), Expect = 4.5e-77
Identity = 159/320 (49.69%), Postives = 218/320 (68.12%), Query Frame = 0 

Query: 8   LAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGARLILPSR 67
           + G  GPSGFGS S+AE VT   +   ++TAIITG T GIG ETARVL+KRGA +++ +R
Sbjct: 7   ITGRRGPSGFGSASTAEEVT-QGIDATNLTAIITGGTGGIGMETARVLSKRGAHVVIGAR 66

Query: 68  NLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLINNAGKFSH 127
           N+ AAE AK  I  + + + +  + LDLSS+ S++ FV +F +L+LPLNLLINNAG    
Sbjct: 67  NMGAAENAKTEILRQNANARVTLLQLDLSSIKSIKAFVREFHALHLPLNLLINNAGVMFC 126

Query: 128 EHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAAATGIQGRIVNVSSSIHGWFSGDII 187
            + +SEDGIE+ FATN++GHFLLT LL++ M  TA  +G++GRI+NVSS  H +   + I
Sbjct: 127 PYQLSEDGIELQFATNHIGHFLLTNLLLDTMKNTAKTSGVEGRILNVSSVAHIYTYQEGI 186

Query: 188 KYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPGIVRTRLT 247
           ++    S N   +Y   RAY  SKLAN+LH +EL+R+L++ G N+T N VHPG++ T L 
Sbjct: 187 QF---DSINDICSYSDKRAYGQSKLANILHANELSRQLQEEGVNITANSVHPGLILTNLF 246

Query: 248 RDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNITGKYFADCNEASPSKIAAS 307
           +     +  L FF +  L K IPQ AATTCYVA +P +  +TGKYFADCNE +PSK+A  
Sbjct: 247 QHTALLMRFLKFF-SFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADCNEVTPSKLARD 306

Query: 308 LTEAARLWSASEIMVNTNSK 328
            T A +LW  S  ++N+ SK
Sbjct: 307 ETLAQKLWDFSVKLINSVSK 321

[Close]
BLAST of Cp4.1LG13g01190 vs. TAIR 10
Match: AT2G37540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 275.4 bits (703), Expect = 6.1e-74
Identity = 159/320 (49.69%), Postives = 211/320 (65.94%), Query Frame = 0 

Query: 8   LAGSPGPSGFGSKSSAEHVTASALHLPSITAIITGATSGIGAETARVLAKRGARLILPSR 67
           + G  G SGFGS S+AE VT  A+    +TAIITG TSGIG E ARVLA RGA +I+ +R
Sbjct: 7   MTGKKGKSGFGSASTAEDVT-QAIDASHLTAIITGGTSGIGLEAARVLAMRGAHVIIAAR 66

Query: 68  NLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLLINNAGKFSH 127
           N KAA E+K  I      + +  + +D+SS+ SVR+FV QF +LN+PLN+LINNAG    
Sbjct: 67  NPKAANESKEMILQMNPNARVDYLQIDVSSIKSVRSFVDQFLALNVPLNILINNAGVMFC 126

Query: 128 EHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAAATGIQGRIVNVSSSIHGWFSGDII 187
              ++EDGIE  FATN++GHFLLT LL++KM  TA  +G+QGRIVN+SS  H +   + I
Sbjct: 127 PFKLTEDGIESQFATNHIGHFLLTNLLLDKMKSTARESGVQGRIVNLSSIAHTYTYSEGI 186

Query: 188 KYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHPGIVRTRLT 247
           K+ G    N    Y   RAY  SKL+N+LH++ L+RRL++ G N+T+N VHPG+V T L 
Sbjct: 187 KFQG---INDPAGYSERRAYGQSKLSNLLHSNALSRRLQEEGVNITINSVHPGLVTTNLF 246

Query: 248 RDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNITGKYFADCNEASPSKIAAS 307
           R   GF   +   +T    K IPQ AATTCYVA +P L  +TGKYF DCN  +PSK A +
Sbjct: 247 R-YSGFSMKVFRAMTFLFWKNIPQGAATTCYVALHPDLEGVTGKYFGDCNIVAPSKFATN 306

Query: 308 LTEAARLWSASEIMVNTNSK 328
            + A +LW  S  ++++ SK
Sbjct: 307 NSLADKLWDFSVFLIDSISK 321

The following BLAST results are available for this feature:
BLAST of Cp4.1LG13g01190 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
A0A078IS661.5e-6948.59Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T... [more]
A0A078ISJ69.8e-6947.96Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T... [more]
A2RVM02.1e-6343.22Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
Q6RVV43.5e-5843.46Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3... [more]
P598372.1e-4240.99Retinol dehydrogenase 12 OS=Bos taurus OX=9913 GN=RDH12 PE=2 SV=1[more]
BLAST of Cp4.1LG13g01190 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023550055.11.38e-233100.00short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita pepo subsp. pepo... [more]
XP_022970067.17.65e-23198.52short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita maxim... [more]
XP_022929143.11.27e-22997.63short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita mosch... [more]
KAG6579556.11.04e-22897.63Short-chain dehydrogenase TIC 32 A, chloroplastic, partial [Cucurbita argyrosper... [more]
XP_022970068.11.36e-22697.92short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita maxim... [more]
BLAST of Cp4.1LG13g01190 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1HY343.70e-23198.52short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita max... [more]
A0A6J1ER896.13e-23097.63short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita mos... [more]
A0A6J1I2S66.60e-22797.92short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita max... [more]
A0A6J1EMX31.09e-22597.03short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita mos... [more]
A0A0A0KKE26.47e-20486.94Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G149870 PE=3 SV=1[more]
BLAST of Cp4.1LG13g01190 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G24050.12.2e-13272.89NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G64590.13.3e-12872.14NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G50130.15.5e-8352.60NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G02540.14.5e-7749.69NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G37540.16.1e-7449.69NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 113..124
score: 44.39
coord: 207..226
score: 31.71
coord: 169..177
score: 54.94
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 207..226
score: 30.81
coord: 37..54
score: 45.52
coord: 230..247
score: 37.54
coord: 163..179
score: 29.89
coord: 113..124
score: 42.05
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 37..177
e-value: 3.7E-28
score: 98.3
NoneNo IPR availableGENE3D3.40.50.720coord: 26..327
e-value: 4.3E-74
score: 251.2
NoneNo IPR availablePANTHERPTHR24320:SF210MITOCHONDRIAL PYRUVATE CARRIERcoord: 4..327
NoneNo IPR availablePANTHERPTHR24320RETINOL DEHYDROGENASEcoord: 4..327
NoneNo IPR availableCDDcd05327retinol-DH_like_SDR_c_likecoord: 37..315
e-value: 1.45545E-86
score: 259.849
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 37..300

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG13g01190.1Cp4.1LG13g01190.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity