Cp4.1LG13g01110 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG13g01110
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionlong chain acyl-CoA synthetase 4-like
LocationCp4.1LG13: 795971 .. 804244 (+)
RNA-Seq ExpressionCp4.1LG13g01110
SyntenyCp4.1LG13g01110
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACTTGTTGAAGTTTCAGAAGAGGAAGAAAAATGCAGAGGAAGTATCTAGTTCAGGTGGAGGAGGGTAAGGAAGCCGCCGCCGGACGGCCGTCGGTCGGGCCGGTTTACCGGAGTGTTTTTGCTAAAGATGGGTTTCCTCCGTCGGTTCAAGGCTTGGAAACCTGCTGGGATATATTTAGGTATGTATTTTTTCTGGTTTGTTAGTCTTTTTTGATGGATGAGAAACCCCAAGAGGAAAATTCTAAAAGTTTAGATAGATTTTAGAATGTGAATTCTGGTTTCTTTGTGTTTTTGTTGAATCTTTGTGGATCTCGCATCGGTTGGAGAAGGGAAGGAAGCATTACTTATAAGCGTGTATAAACCTCTCTATGTCAGACGTGTTTTGAAACCTTGAGGGGAACCCATAAGGGAAAATCCAAAGTATCTGCTCACCGTGGACTTGGGCTGTTTCAAATGGTATCAAAGCAAGACATCGAGCGGTGTGCCAGTGAGGACGCTGGGTCCCCAAGGGGGTGGATTGTGAGATCCCACATCGATTGGAGAGGAGAACGAAACATTCCTTATAAGTGTGGAAACCTCTCCCTATTAGACTGGTTGGGAAAGCCCAAAAAGGACAATATCGGTTAGTGGTGGATTTGAGCTGTTACAAATGGTATCAAAGTCAGACACCGGGTGGTGTGCCAGCAAGGACTTTAGGCCCCAAGTGAGGTGGATTGTGAGATCCCACGTCGGATGGAGAGGGAACGAAATATTCCTTATAAGTGTGTGGAAACCCCTCCTTAATAGACCTGTTTTCAAACCTTGAGGGAGAAGCACAGAAGTGAAAGCCCAAAAATGACAATATCGGCTAGTGGTAGACTTGGGCTATTACAAATGGTATCAAAACCTTGAGGGAGAAGCACAGAAGTGAAAGCCCAAAAATGACAATATCGGCTAGTGGTAGACTTGGGCTATTACAAATGGTATCAAAACCTTGAGGGAGAAGCACAGAAGTGAAAGCCCAAAAATGACAATATCGGCTAGTGGTAGACTTGGGCTATTACAAATGGTATCAAAACCTTGAGGGAGAAGCACAGAAGTGAAAGCCCAAAAATGACAATATCGGCTAGTGGTAGACTTGGGCTATTACAAATGGTATCAAAGTCAGACACTGGGCAGTGTGCCAGTGAGGACTTTAAACCACCCCAAGGGGGATGGATTGTGAGATCCCACGTCGGAAACATTCCTTATAATGGTGTGGCCCCTATGAATTTGTAATTTGTTTCAAGAAAGGTTTGAGGAGCTAGAAATCTTGTATGTTGTAACCAAATATAGGCTCGACTTTACAACTCCGAACTATACATATCGACCTCGTAAATCAAACACTTGTGACCATAATGCTTGATGCAGAATGTCTGTGGAGAAAAATCCCGGAAATCGGATGCTCGGGCGTCGGAAAATAATCGAAGGAAATCTAGTAAGTTACTGATAAATAAGTTGCTTTGGAATTGAACTGAAGAGGGGTAAAACTTTATGGTGGATTATGTGCATCTGCAGGCTAAAGCTGGTGAGTATGAGTGGCTAACTTACCAACAAGTCTATGAATTGGTGTTGAAAATTGGAAACGCAATGCGCAGCCTGGGTTATGGACCAGTAAGTTCTTGAAGATTTTTGTGTTTGTAGAATGCTCTGAGAGTAAATTTGTGGCATGTTTTACTGAGTTTGTAGGGTGAAAAATGTGGAATTTACGGTGCCAATTGTCCTGAGTGGATCATTAGCATGGAGGTATGATTAATGCATTATATCCTTCCTTAGTTCATTCTTAGTTGTATAAATTAATGTAAGATTCTCTTTGGTGTCTTTTTTTGCTTGCAGGCTTGCAATGCTCATGGGCTATATTGTGTTCCTTTATATGATACTTTAGGTATATGTCATTTTATATTGAAAACACACCTTTTAGTCCTTGATGGTTAGGTGCAAGTTTTCGTACGAATTTGTGTTCTTGTTAAACGATTCGTAGAAAAGTAATAAGGGTTAAGTCAATATTTGTGAGATCCCATATCAACTGGAGAGGGGAACGATGCATTCCTCGTAAGGGTGTGGAAACCTCTCCCTAGTGGACGCGTTTTAAAACCTTGAGGGGAAGCTCGGAAGGGAAAGTCCAAAGAGGACAATATTTGTTAACAGTGGACTTGGGTTGTTATAAATGGTATTAGAGCCAGACACCGGGTGGTGTGCCAACGAGAACGCTGGCCCTCAAGGGGGTGGATTGTGAAATCCTANCCTAGCGTCCCTGCTGGCACTCGTTCCCCTCTCCAATCGATGTGGGATCTCACAATCTACCCCCTTCGGGCTAGCGTCCTCGTTAGTACTCCTNCATCGGTTGGAGAGGGGAACGAAGAATTCCTTATAAGGGTGTAGAAAACTCTCTCTAGTGGACATGTTTTAAAACCTTGAGAGGAAGCCCCAAGATGACGGTGGACTTGAGCTGTTATAAATGGTATCAGAGCCAGACATCAGGTGGTGTGCTAGCGAGGATGTTGACCCCTANGCGTCCTCGTTAGTACTCCTTCCTCTCTCTAATCGATGTGGGATCTCACAATCCACCCCCTTCGTGGCTCAGTGTCCTCACTGGCACAGTGCACNTGGACGCATTTTAAAACCTTGAGGGAAAGCCCGGAAGGGAAATTCCAAAGAGGACAATATCTGCTAGAGGTGGACTTGGTCTGTTACAAATGGTGATATCAGAGCCCGACACCGGGTGGTGTGCCAAAGAGGACGTTGGGCCCTCAAGGGGGGTGGATTGTGAGATCCCACATCGGTTGGAGAGGGGAACGAAGCATCCCTCGTAAGGGTGTGGAAACCTCTCTCTAGTGGACGCGTTTTAAAACCTTGAGGGGAAGCCCAGAAGGGAAATTCCAAAGAAGACAATATTTGCTAGCGGTAGACGTGGATTGTTACAATATTTATGCAATATTTGGGGACTAAAATAAAACGTTCAAAAGTTTAGAGATCGAAATAGAATAAGTAAGAAAACGGATCGAAAAACGATGGACGACCCTTCTTAGCCATGTCTCTCAAGTTTTTCAGGTTTGAACATTTTGTAACATGTTTCAGGTGCTGGTGCCATAGAATTCATAATATGCCATGCAGAGACTTCCATTGCTTTTGTAGAAGAGAAGAAGATCCCAGAGGTTCATAGCCATTTCTTTGCACCTAATTAAGAGCCATCTAGTTTCAAGTAAACTGTTTATGATATTAACTTTTAACTCTCTCTTATTCAGCTGTTGAAAACACTTCCCAACACAGCAAATGTTCTTAAAAGTAAGCCCTTGCTCGTTTCTCATACACCACCCAAAATGGCCTGTCTCTAACAGGATTTTAATTTCGCTATGCAGCACTTGTGAGCTTTGGGAAAGTTTCAAATAATCACAAGGAAGAAGTTAACAAATTTGGTTTGGAAATATATTCTTGGGAAGAGTTTCTGCAAAAGGTAAGTTATATTGCTCTCAGTTTTTGGCAGTCTATGGACTAGTAAGGACTCGACTCGTGTCTCGTGTTTGGATTGTGATTAAAAAATAATAGTGTAACAGCTCAAGCCCACCGCTAGTCGATATTGTCCTTTTTGGGCTTTTCCTTTCGGACTTCTCTTTAAGATTTTTAAAACGTGTATGCTAGAGAGAGGTTTTCATACTCTTATAAAGAATGCTTCGTTCTTCTCTCCAACTGATATGAGATCTCACAATCCAACCCCCTTCAGGGCCTAGCGTCCCTGCTGGCACTCGTTCCCCTCTCCAATCGATGTGGGATCTCACAATCTACCCCCTTCGGGCTAGCGTCCTCGTTAGTACTCCTTCCTCTCTCTAATCGATGTGGGATCTCACAATCCACCCCCTTCGTGGCTCAGTGTCCTCACTGGCACAGTGCACAGTGTCTGGCTCTGATACCATTTGTAACAGCCCAAGCCCACCGCTAGCAGATATTGTCCTCCTTGGGTTTCCCTCAGGTTTTTAAAATGCGTCTGCTAGGAAAAGATTTCCACACCCTTATAAAAAATGCTTGGTTCTCCTTCCCAACCTATGTGGGATCTCACCATCCACCCTCCTTCAGGGCACAGTGTCCTCGTTGGCACTCGTTCCCCTCTCCAATCAATGTCATTCCCCTCTCCAATCGATGTGGGATCTCACAAATAGTTTATTTACATCAGTGTTTGATTTGTGAGTATAATTTTTAGAATTAACTTTGAGTATAATTTCTTGTTTTGAAGAACAATTTAGATCATTTAAGACTACTTTTACTGTCATCTATGAACAAAAGTGAGTATCATACATTTTTCTTCACTGAGAATGTTGTTTTTTTTTTACCACTGGAAACAGGGAGATAGCCAACAATATGATCTTCCAGTGAAAGGGAAAGGTGATATTTGCACTATCATGTATACCAGTGGAACAACTGGTGATCCCAAGGGAGTGCTTTTATCCAATAAAGCCATTATAGCTCTTATAGCTGGTGTCAAACATTTGCTTGCAACTGTTAAGGAAGAGGTGATCTCTGTTCTTCCCATTCCCTAACATAACTCAATTTTCAAGCTTGTTGATTCACGAGATGCATATATGTTCGTTGTTCTTGTTATAGGTTCAAGAAAACGACGTTTACATATCATATCTTCCACTCGCGCATATCTTCGACCGGGTGATCGAGGAGGTGTTCATTTTGAATGGTGCCTCCATTGGATTCTGGCGTGGGGTGAGAAATCATATCTTGATCTTTCCCTTTATATGGCAAAGAAATTGAAGAACTTATATCATGTTCATGAGTTTTGTAGGATGTCAAATTGTTAGTTGAAGACATTGGAGAGTTGAAACCAACTATTTTCTGTGCAGTTCCTCGTGTTCTTGAAAGAATTTATGGTGGTGAGCATATGATATGTTGCTTTTGGGTTTGATACTCATTTATCATGCTAGATGTGTTGAATGCAAACCGTTTTAATGTCGTTTCGTCCTCGTTCGATATTAATAGTCTTTGTTCTTAGCTTCGAGCTAAAAGTTGAGCCAATGTGTAAAAAGTATTAGTGTAAGTCGAGTTTTTGCTTATTAGATGGTGGATAACGTCGAGTTTAACGGAATTGTTTAGGTTTGAACCAGAAGATATCGTCGGGTGGCCTCTTGAAAAGATCGTTGTTTAACTTTGCATACTCCTAGTAAGTACATTTTCCCTCTAAATCATTCACTGCTAGTAGATATTGTCATTTTTTAGCTTTCTCTTTTGGGTTTCCCCTCAAGGTTTTAAAAACGCATCTACTAGGGAGAGGTTTCCATACCACTTTAAGGAATGTTTCGTTCCCCTCTCCAACCGATGTGGGATCTCACAATCCACCCTCTTGGGGTCCTAGCGTCCTCGCTAGCACACCGCCCAGTGTCTGGCTCTGATACCATTTGTAACGGCCCAAGGCTACTGCTAGTAGATATTGTCCTCTTTGAACTTTTCCTTGCGGGCTTCCCCTCAAGGTTTTAAAATGCGTCTATTAGGGAGAGGTTTTCATACCCTTATAAGGAATGCTTCGTTTCCTTCTCCAACCGATGTGGGATCTCACAATCTACCCCCTTGGAGTCCCAACGTCCTCGCTAGCACACCGCCTGGTGTCTGGCTCTGATACCATTTGTAACGACTCAAGGCCACTGTTAGTAGATATTGTCCTATTTGGGCTTTCCCTTTTGGGCTTCCCTTCAAGGTTTTAAAATACGTCCACTAGGGAGAGGTTCCCATACTCTTTTAAGGAATGCTTCGTTCCCTCTCCAGCCGATGTGGGATCTCACAGTCCACCCCCCTTGGGGTCCCATCGTCCTCACTGACATACCACCGGTGTCTGGCTCTGATACCATTTGTAACGACCCAAGGCTACTGCTAGTAGGTAGATATTGTCCTGTTTGGGCTTTCCCTTTCGAGCTTCCCTTCCAGGTTTTAAAACACGTCTACTAGGGGGAGGTTCTCATACTCTTTTAAGAAATGTCTTGTTCCCCTCTTCAACCGATGTGGGATCTCACAATCCACCCCCTTGGGGTCCCAGCGTCCTCACTGACACACCGCCTAGTGTCTGCCTCTGATACCATTTGTAACGGCCCAAGGCTACTTCTAGTAGATATTTTCCTCTTCGAGCTTTCCCTTTTGTGCTTCCCCTCAAGGTTTTAAAACGAGTCCACTAGGGAGTGGTTTCCATACCCTTACAAGGAATGATTCGTTCCCGCTCCAACCAACGTAGGATCTCACATTTGAATTCTTCCATGTTGTTCTATCTATATCTTTATCTGTGTACTTTTGCAGCAAATATAACCAAATGCAGAAGGGGAAAAAACATGAACAGGCAGCCACACTCTTTGATAAACTGGTCTTTGACAAGGTAAAAGGGCAATAGACAAGCCATGAGGCTTCACTTCAATCAAAATCATCTAAAAAAATTTAAGAACTCATTCATGTTCTTGCAAGGATATGTTTAGGTGAAGAAGGGATTAGGAGGAAAAGTTCGAATCATTTTATCTGGAGCAGCCCCGCTCGCAGCTCATATCGAAACGTTCTTTCGAGTTGTATCGTGTGCTCATGTTCTACAAGGATATGGTATGTCATCCTTTTACTTCCATTGCCACTAAACAAAGCAAGCAGAGCCCAAATAACATACATTTCTATTGCAGGTTTGACAGAAACTTGTGGTGGAACTTTTGTTACACTACCAAATGAATTGCGAATGTTGGGAACAGTAGGTCCTCCAGTGCCTAATGTTGATATCTGTCTCGAATCAGTACCCGAAATGGGGTACGATTCGCTCGCTAGCACGCCTCGAGGTGAAGTATGTATCAAGGGAGATACATTGTTTGCAGGATACTATAAACGTGAAGATCTTACCACAGAAGTCTTGGTTGATGGATGGTTTCATACAGGTACTCCCTAGTTTTCATGTCGATATTGCTCATGAAAGGTTTGTATGAGATCCCACATCGGTTTGAGAGGGGAATGAAACATTCTTTATAAGGATGTGGAAACCTCTCCCTAATAGACAGGTGAAGCTCAAAGAGGACAATATCTGCTAGCGGTGAGCTTGAGCTATTAAAGTTTGATTCTTACATTCTTTTCTGAATCGTATTAGGGGATGTTGGCGAATGGCAACCAGATGGAAGCTTGAAAATCATTGACCGTAAGAAGAACATCTTCAAACTTTCCCAAGGTGAATATGTTGCAGTCGAAAATCTAGAGCTGATCTATGGCCTTGTTTCCGACATCGAAATGGTATGTATTCTATATCCTAATGGAAAACGAGTCGTGGGCGTCCAAAGACGTCGTCATAACTTTAGCATCAAAACTATGAGATCCCACATCGATTAGAGAGAAACGAGTGCCAGCAAGAACGCTGGGCCTTGAAGGGGGTGGATTGTGAGATCCCACGTCAGTTGGAGAGGAGAACGAAACATTCCTTATAAGGGTGTGGAAACCTCATCCTAGCAGGCCCGTTTTAAAAACTTTGAGGGAAAGCCCAAAAAGGACGATGTCTATTAGCGGTGGACTTGGCCTGTTCTTAGTGAGACCGATAATGTTTGATCGGGATTGTTGCAGATATGGATATATGGAAATAGCTTTGAGGCCTTCCTTGTTGCTGTTGTTAACCCACAAAGGCAAGCACTTGAACATTGGGCAGAAGAAAATGGCATAAAAGGGGATTTCAATGCTCTTTGTGAAGACAAAAGGGCCAAAGACTATATACTTGGACAACTCTCAAAGATTGCCAAAGAAAAAAAGGTCAATTTTTTCTCTCTTTTCTCATGATGATGAACTTGTTGAAAGTAGCTTGAATTGAAGTCGTGGTGGTCGTTTTGCAGCTAAAAGGTTACGAAGTTATCAAAGCGGTTCATCTCGATCCCCTTCCGTTCGATATAGAGCGTGACCTTCTTACTCCATCGTTTAAGAAGAAGAGACCACAAATGCTCAAATACTATCAGGTTCTTCTCGATTCATAGTATTACTTTACACTATAGAGATGATAATGAAACTTTAAAATGAAATTAAAACGGAATGCATAACATAGACTAATAATCGAGCTTTTTTGTTGACAGAAAGAAATCGATGATATGTATAAGGGCGGAAACAAGCCAGTTGCCTAAGGAAGATGAAGATTGAAGAAGGCAAGGATGTAAAGATTCATATTTATATGGAAGTTCAGCTCTTCCCACTTTTTTTTTTTTTTTCTGGTTTGTTTGGATGTAAAGATTCATACTTACTTTTGTTATAATTAATCTGAAAATGGGAAG

mRNA sequence

AACTTGTTGAAGTTTCAGAAGAGGAAGAAAAATGCAGAGGAAGTATCTAGTTCAGGTGGAGGAGGGTAAGGAAGCCGCCGCCGGACGGCCGTCGGTCGGGCCGGTTTACCGGAGTGTTTTTGCTAAAGATGGGTTTCCTCCGTCGGTTCAAGGCTTGGAAACCTGCTGGGATATATTTAGAATGTCTGTGGAGAAAAATCCCGGAAATCGGATGCTCGGGCGTCGGAAAATAATCGAAGGAAATCTAGCTAAAGCTGGTGAGTATGAGTGGCTAACTTACCAACAAGTCTATGAATTGGTGTTGAAAATTGGAAACGCAATGCGCAGCCTGGGTTATGGACCAGGTGAAAAATGTGGAATTTACGGTGCCAATTGTCCTGAGTGGATCATTAGCATGGAGGCTTGCAATGCTCATGGGCTATATTGTGTTCCTTTATATGATACTTTAGGTGCTGGTGCCATAGAATTCATAATATGCCATGCAGAGACTTCCATTGCTTTTGTAGAAGAGAAGAAGATCCCAGAGCTGTTGAAAACACTTCCCAACACAGCAAATGTTCTTAAAACACTTGTGAGCTTTGGGAAAGTTTCAAATAATCACAAGGAAGAAGTTAACAAATTTGGTTTGGAAATATATTCTTGGGAAGAGTTTCTGCAAAAGGGAGATAGCCAACAATATGATCTTCCAGTGAAAGGGAAAGGTGATATTTGCACTATCATGTATACCAGTGGAACAACTGGTGATCCCAAGGGAGTGCTTTTATCCAATAAAGCCATTATAGCTCTTATAGCTGGTGTCAAACATTTGCTTGCAACTGTTAAGGAAGAGGTTCAAGAAAACGACGTTTACATATCATATCTTCCACTCGCGCATATCTTCGACCGGGTGATCGAGGAGGTGTTCATTTTGAATGGTGCCTCCATTGGATTCTGGCGTGGGGATGTCAAATTGTTAGTTGAAGACATTGGAGAGTTGAAACCAACTATTTTCTGTGCAGTTCCTCGTGTTCTTGAAAGAATTTATGGTGGTTTGACAGAAACTTGTGGTGGAACTTTTGTTACACTACCAAATGAATTGCGAATGTTGGGAACAGTAGGTCCTCCAGTGCCTAATGTTGATATCTGTCTCGAATCAGTACCCGAAATGGGGTACGATTCGCTCGCTAGCACGCCTCGAGGTGAAGTATGTATCAAGGGAGATACATTGTTTGCAGGATACTATAAACGTGAAGATCTTACCACAGAAGTCTTGAACATCTTCAAACTTTCCCAAGGTGAATATGTTGCAGTCGAAAATCTAGAGCTGATCTATGGCCTTGTTTCCGACATCGAAATGGCCTTCCTTGTTGCTGTTGTTAACCCACAAAGGCAAGCACTTGAACATTGGGCAGAAGAAAATGGCATAAAAGGGGATTTCAATGCTCTTTGTGAAGACAAAAGGGCCAAAGACTATATACTTGGACAACTCTCAAAGATTGCCAAAGAAAAAAAGCTAAAAGGTTACGAAGTTATCAAAGCGGTTCATCTCGATCCCCTTCCGTTCGATATAGAGCGTGACCTTCTTACTCCATCGTTTAAGAAGAAGAGACCACAAATGCTCAAATACTATCAGAAAGAAATCGATGATATGTATAAGGGCGGAAACAAGCCAGTTGCCTAAGGAAGATGAAGATTGAAGAAGGCAAGGATGTAAAGATTCATATTTATATGGAAGTTCAGCTCTTCCCACTTTTTTTTTTTTTTTCTGGTTTGTTTGGATGTAAAGATTCATACTTACTTTTGTTATAATTAATCTGAAAATGGGAAG

Coding sequence (CDS)

ATGCAGAGGAAGTATCTAGTTCAGGTGGAGGAGGGTAAGGAAGCCGCCGCCGGACGGCCGTCGGTCGGGCCGGTTTACCGGAGTGTTTTTGCTAAAGATGGGTTTCCTCCGTCGGTTCAAGGCTTGGAAACCTGCTGGGATATATTTAGAATGTCTGTGGAGAAAAATCCCGGAAATCGGATGCTCGGGCGTCGGAAAATAATCGAAGGAAATCTAGCTAAAGCTGGTGAGTATGAGTGGCTAACTTACCAACAAGTCTATGAATTGGTGTTGAAAATTGGAAACGCAATGCGCAGCCTGGGTTATGGACCAGGTGAAAAATGTGGAATTTACGGTGCCAATTGTCCTGAGTGGATCATTAGCATGGAGGCTTGCAATGCTCATGGGCTATATTGTGTTCCTTTATATGATACTTTAGGTGCTGGTGCCATAGAATTCATAATATGCCATGCAGAGACTTCCATTGCTTTTGTAGAAGAGAAGAAGATCCCAGAGCTGTTGAAAACACTTCCCAACACAGCAAATGTTCTTAAAACACTTGTGAGCTTTGGGAAAGTTTCAAATAATCACAAGGAAGAAGTTAACAAATTTGGTTTGGAAATATATTCTTGGGAAGAGTTTCTGCAAAAGGGAGATAGCCAACAATATGATCTTCCAGTGAAAGGGAAAGGTGATATTTGCACTATCATGTATACCAGTGGAACAACTGGTGATCCCAAGGGAGTGCTTTTATCCAATAAAGCCATTATAGCTCTTATAGCTGGTGTCAAACATTTGCTTGCAACTGTTAAGGAAGAGGTTCAAGAAAACGACGTTTACATATCATATCTTCCACTCGCGCATATCTTCGACCGGGTGATCGAGGAGGTGTTCATTTTGAATGGTGCCTCCATTGGATTCTGGCGTGGGGATGTCAAATTGTTAGTTGAAGACATTGGAGAGTTGAAACCAACTATTTTCTGTGCAGTTCCTCGTGTTCTTGAAAGAATTTATGGTGGTTTGACAGAAACTTGTGGTGGAACTTTTGTTACACTACCAAATGAATTGCGAATGTTGGGAACAGTAGGTCCTCCAGTGCCTAATGTTGATATCTGTCTCGAATCAGTACCCGAAATGGGGTACGATTCGCTCGCTAGCACGCCTCGAGGTGAAGTATGTATCAAGGGAGATACATTGTTTGCAGGATACTATAAACGTGAAGATCTTACCACAGAAGTCTTGAACATCTTCAAACTTTCCCAAGGTGAATATGTTGCAGTCGAAAATCTAGAGCTGATCTATGGCCTTGTTTCCGACATCGAAATGGCCTTCCTTGTTGCTGTTGTTAACCCACAAAGGCAAGCACTTGAACATTGGGCAGAAGAAAATGGCATAAAAGGGGATTTCAATGCTCTTTGTGAAGACAAAAGGGCCAAAGACTATATACTTGGACAACTCTCAAAGATTGCCAAAGAAAAAAAGCTAAAAGGTTACGAAGTTATCAAAGCGGTTCATCTCGATCCCCTTCCGTTCGATATAGAGCGTGACCTTCTTACTCCATCGTTTAAGAAGAAGAGACCACAAATGCTCAAATACTATCAGAAAGAAATCGATGATATGTATAAGGGCGGAAACAAGCCAGTTGCCTAA

Protein sequence

MQRKYLVQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNRMLGRRKIIEGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWIISMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTLVSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPKGVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGFWRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLTETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLFAGYYKREDLTTEVLNIFKLSQGEYVAVENLELIYGLVSDIEMAFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQLSKIAKEKKLKGYEVIKAVHLDPLPFDIERDLLTPSFKKKRPQMLKYYQKEIDDMYKGGNKPVA
Homology
BLAST of Cp4.1LG13g01110 vs. ExPASy Swiss-Prot
Match: Q9T0A0 (Long chain acyl-CoA synthetase 4 OS=Arabidopsis thaliana OX=3702 GN=LACS4 PE=2 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 5.0e-216
Identity = 377/663 (56.86%), Postives = 458/663 (69.08%), Query Frame = 0

Query: 2   QRKYLVQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNRM 61
           Q+KY+ QVEEGKE + GRPSVGPVYRS+FAKDGFP  ++G+++CWD+FRMSVEK P N M
Sbjct: 4   QKKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRMSVEKYPNNPM 63

Query: 62  LGRRKIIEGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWIIS 121
           LGRR+I++G   K G+Y W TYQ+VY++V+K+GN++RS+G     KCGIYGAN PEWIIS
Sbjct: 64  LGRREIVDG---KPGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPEWIIS 123

Query: 122 MEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTLV 181
           MEACNAHGLYCVPLYDTLGA A+EFII H+E SI FVEEKKI EL KT PN+   +KT+V
Sbjct: 124 MEACNAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNSTEYMKTVV 183

Query: 182 SFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPKG 241
           SFG VS   KEE   FGL IY+W+EFL+ G+ +QYDLP+K K DICTIMYTSGTTGDPKG
Sbjct: 184 SFGGVSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTSGTTGDPKG 243

Query: 242 VLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGFW 301
           V++SN++I+ LIAGV  LL +  E +   DVY+SYLPLAHIFDRVIEE FI +GA+IGFW
Sbjct: 244 VMISNESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFDRVIEECFIQHGAAIGFW 303

Query: 302 RGDVKLLVEDIGELKPTIFCAVPRVLERIYG----------------------------- 361
           RGDVKLL+ED+ ELKPTIFCAVPRVL+R+Y                              
Sbjct: 304 RGDVKLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFIFDSAFSYKFGYMK 363

Query: 362 ----------------------------------------------------------GL 421
                                                                     GL
Sbjct: 364 KGQSHVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVESFLRVVACCHVLQGYGL 423

Query: 422 TETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLFA 481
           TE+C GTFV+LP+EL MLGTVGPPVPNVDI LESVPEM YD+LAST RGE+CI+G TLF+
Sbjct: 424 TESCAGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDALASTARGEICIRGKTLFS 483

Query: 482 GYYKREDLTTEVL--------------------------NIFKLSQGEYVAVENLELIYG 541
           GYYKREDLT EVL                          NIFKLSQGEYVAVEN+E IYG
Sbjct: 484 GYYKREDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIYG 543

Query: 542 LVSDIEM---------AFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQL 543
            V  ++          +FL+A+ NP +  LE WA ENG+ GD++ALC++++AK++ILG+L
Sbjct: 544 EVQAVDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDYDALCQNEKAKEFILGEL 603

BLAST of Cp4.1LG13g01110 vs. ExPASy Swiss-Prot
Match: Q9T009 (Long chain acyl-CoA synthetase 5 OS=Arabidopsis thaliana OX=3702 GN=LACS5 PE=2 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 2.1e-206
Identity = 363/663 (54.75%), Postives = 439/663 (66.21%), Query Frame = 0

Query: 2   QRKYLVQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNRM 61
           Q++++ +VE  KEA  G PSVGPVYRS FA++GFP  + G+++CWDIFR +VEK P NRM
Sbjct: 4   QKRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFRTAVEKYPNNRM 63

Query: 62  LGRRKIIEGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWIIS 121
           LGRR+I  G   KAG+Y W TY++VY++V+K+GN++RS G   GEKCGIYG NC EWIIS
Sbjct: 64  LGRREISNG---KAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCEWIIS 123

Query: 122 MEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTLV 181
           MEACNAHGLYCVPLYDTLGAGA+EFII HAE SIAFVEEKKIPEL KT PN+   +KT+V
Sbjct: 124 MEACNAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYMKTVV 183

Query: 182 SFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPKG 241
           SFG V    KEE  K GL I+SW+EFL+ G+ +QY+LP+K   DICTIMYTSGTTGDPKG
Sbjct: 184 SFGGVKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGTTGDPKG 243

Query: 242 VLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGFW 301
           V++SN++I+ +  GV H L  V   + E DVYISYLPLAH+FDR IEE  I  G SIGFW
Sbjct: 244 VMISNESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFDRAIEECIIQVGGSIGFW 303

Query: 302 RGDVKLLVEDIGELKPTIFCAVPRVLERIYG----------------------------- 361
           RGDVKLL+ED+GELKP+IFCAVPRVL+R+Y                              
Sbjct: 304 RGDVKLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFFKKKVFDVAFSYKFGNMK 363

Query: 362 ----------------------------------------------------------GL 421
                                                                     GL
Sbjct: 364 KGQSHVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIESFLRVVACCNVLQGYGL 423

Query: 422 TETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLFA 481
           TE+C GTF T P+EL MLGTVGPPVPNVDI LESVPEM YD+L STPRGE+CI+G TLF+
Sbjct: 424 TESCAGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDALGSTPRGEICIRGKTLFS 483

Query: 482 GYYKREDLTTEVL--------------------------NIFKLSQGEYVAVENLELIYG 541
           GYYKREDLT EV                           NIFKL+QGEYVAVENLE +Y 
Sbjct: 484 GYYKREDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLAQGEYVAVENLENVYS 543

Query: 542 LVSDIEM---------AFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQL 543
            V  IE          +FLVA+ NP +Q LE WA ENG+ GDFN++C++ +AK +ILG+L
Sbjct: 544 QVEVIESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDFNSICQNAKAKAFILGEL 603

BLAST of Cp4.1LG13g01110 vs. ExPASy Swiss-Prot
Match: Q9C7W4 (Long chain acyl-CoA synthetase 3 OS=Arabidopsis thaliana OX=3702 GN=LACS3 PE=2 SV=1)

HSP 1 Score: 718.0 bits (1852), Expect = 8.0e-206
Identity = 361/655 (55.11%), Postives = 448/655 (68.40%), Query Frame = 0

Query: 4   KYLVQVEEGKEAA-AGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNRML 63
           +Y+V+VE+GK+    G PSVGPVYRS++AKDGFP     L + WDIFR+SVEK+P N ML
Sbjct: 5   RYIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRLSVEKSPNNPML 64

Query: 64  GRRKIIEGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWIISM 123
           GRR+I++G   KAG+Y W TY++V+ +V+K+GN++R++G G G+KCGIYGAN PEWIISM
Sbjct: 65  GRREIVDG---KAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEWIISM 124

Query: 124 EACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTLVS 183
           EACNAHGLYCVPLYDTLGAGAIEFIICHAE S+AF EE KI ELLKT P +   LK +VS
Sbjct: 125 EACNAHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISELLKTAPKSTKYLKYIVS 184

Query: 184 FGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPKGV 243
           FG+V+NN + E  +  L IYSW++FL+ G+ + Y+LP K + D+CTIMYTSGTTGDPKGV
Sbjct: 185 FGEVTNNQRVEAERHRLTIYSWDQFLKLGEGKHYELPEKRRSDVCTIMYTSGTTGDPKGV 244

Query: 244 LLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGFWR 303
           LL+N++II L+ GVK LL T+ EE+   DVY+SYLPLAHIFDRVIEE+ I   ASIGFWR
Sbjct: 245 LLTNESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEELCIYEAASIGFWR 304

Query: 304 GDVKLLVEDIGELKPTIFCAVPRVLERIYG------------------------------ 363
           GDVK+L+EDI  LKPT+FCAVPRVLERIY                               
Sbjct: 305 GDVKILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLFNFAFKYKHKNMEK 364

Query: 364 ---------------------------------------------------------GLT 423
                                                                    GLT
Sbjct: 365 GQPHEQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRVVACAHVLQGYGLT 424

Query: 424 ETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLFAG 483
           E+CGGTFV++PNEL MLGTVGPPVPNVDI LESVPEMGYD+LAS PRGE+CI+G TLF+G
Sbjct: 425 ESCGGTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPRGEICIRGKTLFSG 484

Query: 484 YYKREDLTTEVL--------------------------NIFKLSQGEYVAVENLELIYGL 536
           YYKREDLT EV                           NIFKLSQGEYVAVENLE IY  
Sbjct: 485 YYKREDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGEYVAVENLENIYSH 544

BLAST of Cp4.1LG13g01110 vs. ExPASy Swiss-Prot
Match: M4ISH1 (Probable CoA ligase CCL6 OS=Humulus lupulus OX=3486 GN=CCL6 PE=2 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 1.3e-155
Identity = 283/652 (43.40%), Postives = 396/652 (60.74%), Query Frame = 0

Query: 5   YLVQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNRMLGR 64
           Y V+VEE + A    PS GPVYRS++A+DG     +GL++ W+    SV+++P   MLGR
Sbjct: 3   YTVKVEEARPATEKMPSAGPVYRSIYARDGLLELPEGLQSPWEFLSGSVKRSPKTPMLGR 62

Query: 65  RKIIEGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWIISMEA 124
           R+I +   ++AG Y WLTYQ+V++  +++ +AMRS G  PG++CGIYG NCP+WI++M+A
Sbjct: 63  RQIKD---SEAGPYVWLTYQEVHDEAIRMASAMRSRGVNPGDRCGIYGTNCPQWIVAMQA 122

Query: 125 CNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTLVSFG 184
           C +H +  VPLYDTLG  A+EFII H E SIAFV+E KI  +L  LPN +++LKT+VSFG
Sbjct: 123 CYSHAITYVPLYDTLGPNAVEFIINHGEVSIAFVQENKISAILSCLPNCSSLLKTIVSFG 182

Query: 185 KVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPKGVLL 244
            +++  K+E    G+  +SWEEF Q G+    +LP K + DICT+MYTSG TG+PKGV+L
Sbjct: 183 NITSVQKKETEALGVSCFSWEEFSQLGNLSG-ELPEKHRTDICTLMYTSGATGEPKGVIL 242

Query: 245 SNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGFWRGD 304
           +N+AI+A I    ++L    +   E DVY SYLPLAH++D+++E   I  G++IG+WRGD
Sbjct: 243 TNEAIMAEILSTDNMLELTDKVFSEEDVYFSYLPLAHVYDQIVENYCIYKGSAIGYWRGD 302

Query: 305 VKLLVEDIGELKPTIFCAVPRVLERIYG-------------------------------- 364
           ++ L++D+ ELKPTIFC VPRV +RIY                                 
Sbjct: 303 IRFLMDDLQELKPTIFCGVPRVYDRIYAGIFHKVSSGGTLKKMLFQYAYNYKMANMEKGL 362

Query: 365 -------------------------------------------------------GLTET 424
                                                                  GLTE+
Sbjct: 363 PHGQAAPLMDKLFFDKIKQGFGGRIRLMFSGAAPLPHHVEEYLRVTSCAALSQGYGLTES 422

Query: 425 CGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLFAGYY 484
           CGG   ++ N   M+GTVG P+  ++  LESVPEMGYD+L+  PRGE+C++G TLF+GY+
Sbjct: 423 CGGCLTSIANIFPMIGTVGVPMTTIEARLESVPEMGYDALSDKPRGEICLRGTTLFSGYH 482

Query: 485 KREDLTTEVL--------------------------NIFKLSQGEYVAVENLELIY---G 535
           KREDLT +VL                          NIFKLSQGEYV VEN+E IY    
Sbjct: 483 KREDLTKDVLVDGWFHTGDIGEWQPNGAMKIIDRKKNIFKLSQGEYVPVENIEGIYLQCP 542

BLAST of Cp4.1LG13g01110 vs. ExPASy Swiss-Prot
Match: Q9XIA9 (Long chain acyl-CoA synthetase 2 OS=Arabidopsis thaliana OX=3702 GN=LACS2 PE=2 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 6.0e-145
Identity = 271/658 (41.19%), Postives = 382/658 (58.05%), Query Frame = 0

Query: 9   VEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNRMLGRRKII 68
           VEEG+ A A  PS GPVYR  +AKDG       +++ W  F  +V+K P  +MLG+R   
Sbjct: 11  VEEGRPATAEHPSAGPVYRCKYAKDGLLDLPTDIDSPWQFFSEAVKKYPNEQMLGQRVTT 70

Query: 69  EGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWIISMEACNAH 128
           +   +K G Y W+TY++ ++  ++IG+A+RS G  PG  CGIYGANCPEWII+MEAC + 
Sbjct: 71  D---SKVGPYTWITYKEAHDAAIRIGSAIRSRGVDPGHCCGIYGANCPEWIIAMEACMSQ 130

Query: 129 GLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTLVSFGKVSN 188
           G+  VPLYD+LG  A+EFII HAE S+ FV+EK +  +L      ++ LKT+VSFG+VS+
Sbjct: 131 GITYVPLYDSLGVNAVEFIINHAEVSLVFVQEKTVSSILSCQKGCSSNLKTIVSFGEVSS 190

Query: 189 NHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPKGVLLSNKA 248
             KEE     + ++SW EF   G+  + +LP K K DICTIMYTSGTTG+PKGV+L+N A
Sbjct: 191 TQKEEAKNQCVSLFSWNEFSLMGNLDEANLPRKRKTDICTIMYTSGTTGEPKGVILNNAA 250

Query: 249 IIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGFWRGDVKLL 308
           I   +  +  +L         +DV+ SYLPLAH +D+V+E  F+  G+S+G+WRGD++ L
Sbjct: 251 ISVQVLSIDKMLEVTDRSCDTSDVFFSYLPLAHCYDQVMEIYFLSRGSSVGYWRGDIRYL 310

Query: 309 VEDIGELKPTIFCAVPRVLERIYG------------------------------------ 368
           ++D+  LKPT+FC VPRV +++Y                                     
Sbjct: 311 MDDVQALKPTVFCGVPRVYDKLYAGIMQKISASGLIRKKLFDFAYNYKLGNMRKGFSQEE 370

Query: 369 ---------------------------------------------------GLTETCGGT 428
                                                              GLTE+CGG+
Sbjct: 371 ASPRLDRLMFDKIKEALGGRAHMLLSGAAPLPRHVEEFLRIIPASNLSQGYGLTESCGGS 430

Query: 429 FVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSL-ASTPRGEVCIKGDTLFAGYYKRE 488
           F TL     M+GTVG P+P V+  L SVPEMGYD+  A  PRGE+C++G+++F+GY+KR+
Sbjct: 431 FTTLAGVFSMVGTVGVPMPTVEARLVSVPEMGYDAFSADVPRGEICLRGNSMFSGYHKRQ 490

Query: 489 DLTTEVL--------------------------NIFKLSQGEYVAVENLELIYG---LVS 543
           DLT +VL                          NIFKLSQGEYVAVENLE  Y    L++
Sbjct: 491 DLTDQVLIDGWFHTGDIGEWQEDGSMKIIDRKKNIFKLSQGEYVAVENLENTYSRCPLIA 550

BLAST of Cp4.1LG13g01110 vs. NCBI nr
Match: XP_023549978.1 (long chain acyl-CoA synthetase 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1042 bits (2695), Expect = 0.0
Identity = 542/664 (81.63%), Postives = 542/664 (81.63%), Query Frame = 0

Query: 1   MQRKYLVQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNR 60
           MQRKYLVQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNR
Sbjct: 1   MQRKYLVQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIIEGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII 120
           MLGRRKIIEGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII
Sbjct: 61  MLGRRKIIEGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTL 180
           SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTL
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTL 180

Query: 181 VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240
           VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK
Sbjct: 181 VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300
           GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF
Sbjct: 241 GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGL-------------------------- 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGL                          
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGLLKRSLFNFAYSYKYNQM 360

Query: 361 ------------------------------------------------------------ 420
                                                                       
Sbjct: 361 QKGKKHEQAATLFDKLVFDKVKKGLGGKVRIILSGAAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 -TETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF 480
            TETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF 480

Query: 481 AGYYKREDLTTEVL--------------------------NIFKLSQGEYVAVENLELIY 540
           AGYYKREDLTTEVL                          NIFKLSQGEYVAVENLELIY
Sbjct: 481 AGYYKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVSDIEM---------AFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ 542
           GLVSDIEM         AFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGNSFEAFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ 600

BLAST of Cp4.1LG13g01110 vs. NCBI nr
Match: KAG6579555.1 (Long chain acyl-CoA synthetase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1032 bits (2669), Expect = 0.0
Identity = 536/664 (80.72%), Postives = 539/664 (81.17%), Query Frame = 0

Query: 1   MQRKYLVQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNR 60
           MQRKYL QVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSV+GLETCWDIFRMSVEKNPGNR
Sbjct: 1   MQRKYLFQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVEGLETCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIIEGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII 120
           MLGRRKI+EGN AKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII
Sbjct: 61  MLGRRKIVEGNPAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTL 180
           SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTL
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTL 180

Query: 181 VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240
           VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGD QQYDLPVKGKGDICTIMYTSGTTGDPK
Sbjct: 181 VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDIQQYDLPVKGKGDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300
           GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF
Sbjct: 241 GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGL-------------------------- 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGL                          
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGLLKRSLFNFAYSYKYNQM 360

Query: 361 ------------------------------------------------------------ 420
                                                                       
Sbjct: 361 QKGKKHEQAATLFDKLVFDKVKKGLGGKVRIILSGAAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 -TETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF 480
            TETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF 480

Query: 481 AGYYKREDLTTEVL--------------------------NIFKLSQGEYVAVENLELIY 540
           AGYYKREDLTTEVL                          NIFKLSQGEYVAVENLELIY
Sbjct: 481 AGYYKREDLTTEVLVDGWFHTGDVGEWQTDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVSDIEM---------AFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ 542
           GLVSDIEM         AFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGNSFEAFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ 600

BLAST of Cp4.1LG13g01110 vs. NCBI nr
Match: XP_022929138.1 (long chain acyl-CoA synthetase 4-like [Cucurbita moschata])

HSP 1 Score: 1032 bits (2668), Expect = 0.0
Identity = 536/664 (80.72%), Postives = 539/664 (81.17%), Query Frame = 0

Query: 1   MQRKYLVQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNR 60
           MQRKYL QVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSV+GLETCWDIFRMSVEKNPGNR
Sbjct: 1   MQRKYLFQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVEGLETCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIIEGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII 120
           MLGRRKI+EGN AKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII
Sbjct: 61  MLGRRKIVEGNPAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTL 180
           SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTL
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTL 180

Query: 181 VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240
           VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYD PVKGKGDICTIMYTSGTTGDPK
Sbjct: 181 VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDPPVKGKGDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300
           GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF
Sbjct: 241 GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGL-------------------------- 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGL                          
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGLLKRSLFNFAYSYKYNQM 360

Query: 361 ------------------------------------------------------------ 420
                                                                       
Sbjct: 361 QKGKKHEQAATLFDKLVFDKVKKGLGGKVRIILSGAAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 -TETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF 480
            TETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF 480

Query: 481 AGYYKREDLTTEVL--------------------------NIFKLSQGEYVAVENLELIY 540
           AGYYKREDLTTEVL                          NIFKLSQGEYVAVENLELIY
Sbjct: 481 AGYYKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVSDIEM---------AFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ 542
           GLVSDIEM         AFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGNSFEAFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ 600

BLAST of Cp4.1LG13g01110 vs. NCBI nr
Match: XP_022970006.1 (long chain acyl-CoA synthetase 4-like [Cucurbita maxima])

HSP 1 Score: 1027 bits (2656), Expect = 0.0
Identity = 532/664 (80.12%), Postives = 538/664 (81.02%), Query Frame = 0

Query: 1   MQRKYLVQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNR 60
           MQ KYL QVEEGKEAAAGRPSVGPVYRS+FAKDGFPPSVQGLETCWDIFRMSVEKNPGNR
Sbjct: 1   MQGKYLFQVEEGKEAAAGRPSVGPVYRSIFAKDGFPPSVQGLETCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIIEGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII 120
           MLG RKI+EGN AKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII
Sbjct: 61  MLGHRKIVEGNPAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTL 180
           SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEE+KIPELLKTLPNTANVLKTL
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEERKIPELLKTLPNTANVLKTL 180

Query: 181 VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240
           VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK
Sbjct: 181 VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300
           GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF
Sbjct: 241 GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGL-------------------------- 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGL                          
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGLLKRSLFNFAYSYKYNQM 360

Query: 361 ------------------------------------------------------------ 420
                                                                       
Sbjct: 361 QKGKKHEQAATLFDKLVFDKVKKGLGGKVRIILSGAAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 -TETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF 480
            TETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF 480

Query: 481 AGYYKREDLTTEVL--------------------------NIFKLSQGEYVAVENLELIY 540
           AGYYKREDLTTEVL                          NIFKLSQGEYVAVENLELIY
Sbjct: 481 AGYYKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVSDIEM---------AFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ 542
           GLVSDIEM         +FLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGNSFESFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ 600

BLAST of Cp4.1LG13g01110 vs. NCBI nr
Match: XP_004143868.1 (long chain acyl-CoA synthetase 4 [Cucumis sativus])

HSP 1 Score: 974 bits (2519), Expect = 0.0
Identity = 502/664 (75.60%), Postives = 524/664 (78.92%), Query Frame = 0

Query: 1   MQRKYLVQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNR 60
           MQRKYLVQVEE K+A AGRPSVGPVYRS+FAKDGFPP VQGL+TCWDIFRMSVEKNPGNR
Sbjct: 1   MQRKYLVQVEEAKDATAGRPSVGPVYRSIFAKDGFPPPVQGLDTCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIIEGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII 120
           MLGRRK++EGN AKAGEYEWLTY++VYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII
Sbjct: 61  MLGRRKVVEGNPAKAGEYEWLTYKEVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTL 180
           SMEACNAHGLYCVPLYDTLGAGAIEFIICHAE +IAFVEEKKI ELLKTLPNTA  LKT+
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEIAIAFVEEKKISELLKTLPNTAKFLKTI 180

Query: 181 VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240
           VSFGKVS+NHKEEVNKFGLEIY+WEEFLQKGDSQQYDLPVK K DICTIMYTSGTTGDPK
Sbjct: 181 VSFGKVSDNHKEEVNKFGLEIYTWEEFLQKGDSQQYDLPVKEKSDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300
           GVLLSN AIIALIAGVKHLL TVKE VQENDVYISYLPLAHIFDRVIEEVFILNGASIGF
Sbjct: 241 GVLLSNTAIIALIAGVKHLLTTVKENVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGL-------------------------- 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGL                          
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKRSLFNFAYSYKYNQM 360

Query: 361 ------------------------------------------------------------ 420
                                                                       
Sbjct: 361 QKGKTHQQAAPLFDKVVFDKVKKGLGGNVRIILSGGAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 -TETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF 480
            TETCGGTFV+LP+EL MLGTVGPPVPNVDICLESVPEMGYDS ASTPRGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVSLPDELPMLGTVGPPVPNVDICLESVPEMGYDSHASTPRGEVCIKGDTLF 480

Query: 481 AGYYKREDLTTEVL--------------------------NIFKLSQGEYVAVENLELIY 540
           +GYYKR+DLTTEVL                          NIFKLSQGEYVAVENLELIY
Sbjct: 481 SGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVSDIEM---------AFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ 542
           GLVSDIEM         +FLVAVVNP++QALE WAEENGIKGDFNALCEDKRAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGNSFESFLVAVVNPKKQALEQWAEENGIKGDFNALCEDKRAKDYILGQ 600

BLAST of Cp4.1LG13g01110 vs. ExPASy TrEMBL
Match: A0A6J1ER85 (long chain acyl-CoA synthetase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111435809 PE=4 SV=1)

HSP 1 Score: 1032 bits (2668), Expect = 0.0
Identity = 536/664 (80.72%), Postives = 539/664 (81.17%), Query Frame = 0

Query: 1   MQRKYLVQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNR 60
           MQRKYL QVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSV+GLETCWDIFRMSVEKNPGNR
Sbjct: 1   MQRKYLFQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVEGLETCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIIEGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII 120
           MLGRRKI+EGN AKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII
Sbjct: 61  MLGRRKIVEGNPAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTL 180
           SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTL
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTL 180

Query: 181 VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240
           VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYD PVKGKGDICTIMYTSGTTGDPK
Sbjct: 181 VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDPPVKGKGDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300
           GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF
Sbjct: 241 GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGL-------------------------- 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGL                          
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGLLKRSLFNFAYSYKYNQM 360

Query: 361 ------------------------------------------------------------ 420
                                                                       
Sbjct: 361 QKGKKHEQAATLFDKLVFDKVKKGLGGKVRIILSGAAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 -TETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF 480
            TETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF 480

Query: 481 AGYYKREDLTTEVL--------------------------NIFKLSQGEYVAVENLELIY 540
           AGYYKREDLTTEVL                          NIFKLSQGEYVAVENLELIY
Sbjct: 481 AGYYKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVSDIEM---------AFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ 542
           GLVSDIEM         AFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGNSFEAFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ 600

BLAST of Cp4.1LG13g01110 vs. ExPASy TrEMBL
Match: A0A6J1HZD4 (long chain acyl-CoA synthetase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469025 PE=4 SV=1)

HSP 1 Score: 1027 bits (2656), Expect = 0.0
Identity = 532/664 (80.12%), Postives = 538/664 (81.02%), Query Frame = 0

Query: 1   MQRKYLVQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNR 60
           MQ KYL QVEEGKEAAAGRPSVGPVYRS+FAKDGFPPSVQGLETCWDIFRMSVEKNPGNR
Sbjct: 1   MQGKYLFQVEEGKEAAAGRPSVGPVYRSIFAKDGFPPSVQGLETCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIIEGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII 120
           MLG RKI+EGN AKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII
Sbjct: 61  MLGHRKIVEGNPAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTL 180
           SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEE+KIPELLKTLPNTANVLKTL
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEERKIPELLKTLPNTANVLKTL 180

Query: 181 VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240
           VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK
Sbjct: 181 VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300
           GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF
Sbjct: 241 GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGL-------------------------- 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGL                          
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGLLKRSLFNFAYSYKYNQM 360

Query: 361 ------------------------------------------------------------ 420
                                                                       
Sbjct: 361 QKGKKHEQAATLFDKLVFDKVKKGLGGKVRIILSGAAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 -TETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF 480
            TETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF 480

Query: 481 AGYYKREDLTTEVL--------------------------NIFKLSQGEYVAVENLELIY 540
           AGYYKREDLTTEVL                          NIFKLSQGEYVAVENLELIY
Sbjct: 481 AGYYKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVSDIEM---------AFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ 542
           GLVSDIEM         +FLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGNSFESFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ 600

BLAST of Cp4.1LG13g01110 vs. ExPASy TrEMBL
Match: A0A5D3C2P4 (Long chain acyl-CoA synthetase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001880 PE=4 SV=1)

HSP 1 Score: 963 bits (2490), Expect = 0.0
Identity = 498/664 (75.00%), Postives = 519/664 (78.16%), Query Frame = 0

Query: 1   MQRKYLVQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNR 60
           MQRKYLVQVEE K+A AGRPSVGPVYRS+FAKDGFPP VQGL+TCWDIFRMSVEKNPGNR
Sbjct: 1   MQRKYLVQVEEAKDATAGRPSVGPVYRSIFAKDGFPPPVQGLDTCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIIEGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII 120
           MLGRRK++EGN AKAGEYEWLTY++VYELVLKIGNAMR LGYGPGEKCGIYGANCPEWII
Sbjct: 61  MLGRRKVVEGNPAKAGEYEWLTYKEVYELVLKIGNAMRGLGYGPGEKCGIYGANCPEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTL 180
           SMEACNAHGLYCVPLYDTLGAGAIEFII HAE SIAFVEEKKI ELLKTLPNTA  LKT+
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIISHAEISIAFVEEKKISELLKTLPNTAKFLKTI 180

Query: 181 VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240
           VSFGKVS+NHKEEV KFGLEIY+WEEFLQKGDSQQ+DLPVK K DICTIMYTSGTTGDPK
Sbjct: 181 VSFGKVSDNHKEEVKKFGLEIYTWEEFLQKGDSQQFDLPVKEKSDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300
           GVLLSN AIIALIAGVKHLL TV E VQENDVYISYLPLAHIFDRVIEEVFILNGASIGF
Sbjct: 241 GVLLSNNAIIALIAGVKHLLTTVNENVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGL-------------------------- 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGL                          
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKRSLFNLAYSYKYNQM 360

Query: 361 ------------------------------------------------------------ 420
                                                                       
Sbjct: 361 QKGKKHQQAAPLFDKVVFDKVKKGLGGNVRIILSGGAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 -TETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF 480
            TETCGGTFVTLP+EL MLGTVGPPVPNVDICLESVPEMGYDS ASTPRGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVTLPDELPMLGTVGPPVPNVDICLESVPEMGYDSHASTPRGEVCIKGDTLF 480

Query: 481 AGYYKREDLTTEVL--------------------------NIFKLSQGEYVAVENLELIY 540
           +GYYKR+DLTTEVL                          NIFKLSQGEYVAVENLELIY
Sbjct: 481 SGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVSDIEM---------AFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ 542
           GLVSDIEM         +FLVAVVNP++QALE WAEENGIK DFNALCEDKRAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGNSFESFLVAVVNPKKQALEQWAEENGIKEDFNALCEDKRAKDYILGQ 600

BLAST of Cp4.1LG13g01110 vs. ExPASy TrEMBL
Match: A0A1S3AUH9 (long chain acyl-CoA synthetase 4-like OS=Cucumis melo OX=3656 GN=LOC103482821 PE=4 SV=1)

HSP 1 Score: 963 bits (2490), Expect = 0.0
Identity = 498/664 (75.00%), Postives = 519/664 (78.16%), Query Frame = 0

Query: 1   MQRKYLVQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNR 60
           MQRKYLVQVEE K+A AGRPSVGPVYRS+FAKDGFPP VQGL+TCWDIFRMSVEKNPGNR
Sbjct: 1   MQRKYLVQVEEAKDATAGRPSVGPVYRSIFAKDGFPPPVQGLDTCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIIEGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII 120
           MLGRRK++EGN AKAGEYEWLTY++VYELVLKIGNAMR LGYGPGEKCGIYGANCPEWII
Sbjct: 61  MLGRRKVVEGNPAKAGEYEWLTYKEVYELVLKIGNAMRGLGYGPGEKCGIYGANCPEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTL 180
           SMEACNAHGLYCVPLYDTLGAGAIEFII HAE SIAFVEEKKI ELLKTLPNTA  LKT+
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIISHAEISIAFVEEKKISELLKTLPNTAKFLKTI 180

Query: 181 VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240
           VSFGKVS+NHKEEV KFGLEIY+WEEFLQKGDSQQ+DLPVK K DICTIMYTSGTTGDPK
Sbjct: 181 VSFGKVSDNHKEEVKKFGLEIYTWEEFLQKGDSQQFDLPVKEKSDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300
           GVLLSN AIIALIAGVKHLL TV E VQENDVYISYLPLAHIFDRVIEEVFILNGASIGF
Sbjct: 241 GVLLSNNAIIALIAGVKHLLTTVNENVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGL-------------------------- 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGL                          
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKRSLFNLAYSYKYNQM 360

Query: 361 ------------------------------------------------------------ 420
                                                                       
Sbjct: 361 QKGKKHQQAAPLFDKVVFDKVKKGLGGNVRIILSGGAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 -TETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF 480
            TETCGGTFVTLP+EL MLGTVGPPVPNVDICLESVPEMGYDS ASTPRGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVTLPDELPMLGTVGPPVPNVDICLESVPEMGYDSHASTPRGEVCIKGDTLF 480

Query: 481 AGYYKREDLTTEVL--------------------------NIFKLSQGEYVAVENLELIY 540
           +GYYKR+DLTTEVL                          NIFKLSQGEYVAVENLELIY
Sbjct: 481 SGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVSDIEM---------AFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ 542
           GLVSDIEM         +FLVAVVNP++QALE WAEENGIK DFNALCEDKRAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGNSFESFLVAVVNPKKQALEQWAEENGIKEDFNALCEDKRAKDYILGQ 600

BLAST of Cp4.1LG13g01110 vs. ExPASy TrEMBL
Match: A0A5A7TH75 (Long chain acyl-CoA synthetase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G001820 PE=4 SV=1)

HSP 1 Score: 952 bits (2461), Expect = 0.0
Identity = 497/689 (72.13%), Postives = 519/689 (75.33%), Query Frame = 0

Query: 1   MQRKYLVQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNR 60
           MQRKYLVQVEE K+A AGRPSVGPVYRS+FAKDGFPP VQGL+TCWDIFRMSVEKNPGNR
Sbjct: 1   MQRKYLVQVEEAKDATAGRPSVGPVYRSIFAKDGFPPPVQGLDTCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIIEGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII 120
           MLGRRK++EGN AKAGEYEWLTY++VYELVLKIGNAMR LGYGPGEKCGIYGANCPEWII
Sbjct: 61  MLGRRKVVEGNPAKAGEYEWLTYKEVYELVLKIGNAMRGLGYGPGEKCGIYGANCPEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTL 180
           SMEACNAHGLYCVPLYDTLGAGAIEFII HAE SIAFVEEKKI ELLKTLPNTA  LKT+
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIISHAEISIAFVEEKKISELLKTLPNTAKFLKTI 180

Query: 181 VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240
           VSFGKVS+NHKEEV KFGLEIY+WEEFLQKGDSQQ+DLPVK K DICTIMYTSGTTGDPK
Sbjct: 181 VSFGKVSDNHKEEVKKFGLEIYTWEEFLQKGDSQQFDLPVKEKSDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300
           GVLLSN AIIALIAGVKHLL TV E VQENDVYISYLPLAHIFDRVIEEVFILNGASIGF
Sbjct: 241 GVLLSNNAIIALIAGVKHLLTTVNENVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGL-------------------------- 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGL                          
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKRSLFNLAYSYKYNQM 360

Query: 361 ------------------------------------------------------------ 420
                                                                       
Sbjct: 361 QKGKKHQQAAPLFDKVVFDKVKKGLGGNVRIILSGGAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 --------------------------TETCGGTFVTLPNELRMLGTVGPPVPNVDICLES 480
                                     TETCGGTFVTLP+EL MLGTVGPPVPNVDICLES
Sbjct: 421 ISSLYLHFQKPKQREPKYHTFLFAGLTETCGGTFVTLPDELPMLGTVGPPVPNVDICLES 480

Query: 481 VPEMGYDSLASTPRGEVCIKGDTLFAGYYKREDLTTEVL--------------------- 540
           VPEMGYDS ASTPRGEVCIKGDTLF+GYYKR+DLTTEVL                     
Sbjct: 481 VPEMGYDSHASTPRGEVCIKGDTLFSGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKI 540

Query: 541 -----NIFKLSQGEYVAVENLELIYGLVSDIEM---------AFLVAVVNPQRQALEHWA 542
                NIFKLSQGEYVAVENLELIYGLVSDIEM         +FLVAVVNP++QALE WA
Sbjct: 541 IDRKKNIFKLSQGEYVAVENLELIYGLVSDIEMIWIYGNSFESFLVAVVNPKKQALEQWA 600

BLAST of Cp4.1LG13g01110 vs. TAIR 10
Match: AT4G23850.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 751.9 bits (1940), Expect = 3.6e-217
Identity = 377/663 (56.86%), Postives = 458/663 (69.08%), Query Frame = 0

Query: 2   QRKYLVQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNRM 61
           Q+KY+ QVEEGKE + GRPSVGPVYRS+FAKDGFP  ++G+++CWD+FRMSVEK P N M
Sbjct: 4   QKKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRMSVEKYPNNPM 63

Query: 62  LGRRKIIEGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWIIS 121
           LGRR+I++G   K G+Y W TYQ+VY++V+K+GN++RS+G     KCGIYGAN PEWIIS
Sbjct: 64  LGRREIVDG---KPGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPEWIIS 123

Query: 122 MEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTLV 181
           MEACNAHGLYCVPLYDTLGA A+EFII H+E SI FVEEKKI EL KT PN+   +KT+V
Sbjct: 124 MEACNAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNSTEYMKTVV 183

Query: 182 SFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPKG 241
           SFG VS   KEE   FGL IY+W+EFL+ G+ +QYDLP+K K DICTIMYTSGTTGDPKG
Sbjct: 184 SFGGVSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTSGTTGDPKG 243

Query: 242 VLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGFW 301
           V++SN++I+ LIAGV  LL +  E +   DVY+SYLPLAHIFDRVIEE FI +GA+IGFW
Sbjct: 244 VMISNESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFDRVIEECFIQHGAAIGFW 303

Query: 302 RGDVKLLVEDIGELKPTIFCAVPRVLERIYG----------------------------- 361
           RGDVKLL+ED+ ELKPTIFCAVPRVL+R+Y                              
Sbjct: 304 RGDVKLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFIFDSAFSYKFGYMK 363

Query: 362 ----------------------------------------------------------GL 421
                                                                     GL
Sbjct: 364 KGQSHVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVESFLRVVACCHVLQGYGL 423

Query: 422 TETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLFA 481
           TE+C GTFV+LP+EL MLGTVGPPVPNVDI LESVPEM YD+LAST RGE+CI+G TLF+
Sbjct: 424 TESCAGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDALASTARGEICIRGKTLFS 483

Query: 482 GYYKREDLTTEVL--------------------------NIFKLSQGEYVAVENLELIYG 541
           GYYKREDLT EVL                          NIFKLSQGEYVAVEN+E IYG
Sbjct: 484 GYYKREDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIYG 543

Query: 542 LVSDIEM---------AFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQL 543
            V  ++          +FL+A+ NP +  LE WA ENG+ GD++ALC++++AK++ILG+L
Sbjct: 544 EVQAVDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDYDALCQNEKAKEFILGEL 603

BLAST of Cp4.1LG13g01110 vs. TAIR 10
Match: AT4G11030.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 719.9 bits (1857), Expect = 1.5e-207
Identity = 363/663 (54.75%), Postives = 439/663 (66.21%), Query Frame = 0

Query: 2   QRKYLVQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNRM 61
           Q++++ +VE  KEA  G PSVGPVYRS FA++GFP  + G+++CWDIFR +VEK P NRM
Sbjct: 4   QKRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFRTAVEKYPNNRM 63

Query: 62  LGRRKIIEGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWIIS 121
           LGRR+I  G   KAG+Y W TY++VY++V+K+GN++RS G   GEKCGIYG NC EWIIS
Sbjct: 64  LGRREISNG---KAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCEWIIS 123

Query: 122 MEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTLV 181
           MEACNAHGLYCVPLYDTLGAGA+EFII HAE SIAFVEEKKIPEL KT PN+   +KT+V
Sbjct: 124 MEACNAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYMKTVV 183

Query: 182 SFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPKG 241
           SFG V    KEE  K GL I+SW+EFL+ G+ +QY+LP+K   DICTIMYTSGTTGDPKG
Sbjct: 184 SFGGVKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGTTGDPKG 243

Query: 242 VLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGFW 301
           V++SN++I+ +  GV H L  V   + E DVYISYLPLAH+FDR IEE  I  G SIGFW
Sbjct: 244 VMISNESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFDRAIEECIIQVGGSIGFW 303

Query: 302 RGDVKLLVEDIGELKPTIFCAVPRVLERIYG----------------------------- 361
           RGDVKLL+ED+GELKP+IFCAVPRVL+R+Y                              
Sbjct: 304 RGDVKLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFFKKKVFDVAFSYKFGNMK 363

Query: 362 ----------------------------------------------------------GL 421
                                                                     GL
Sbjct: 364 KGQSHVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIESFLRVVACCNVLQGYGL 423

Query: 422 TETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLFA 481
           TE+C GTF T P+EL MLGTVGPPVPNVDI LESVPEM YD+L STPRGE+CI+G TLF+
Sbjct: 424 TESCAGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDALGSTPRGEICIRGKTLFS 483

Query: 482 GYYKREDLTTEVL--------------------------NIFKLSQGEYVAVENLELIYG 541
           GYYKREDLT EV                           NIFKL+QGEYVAVENLE +Y 
Sbjct: 484 GYYKREDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLAQGEYVAVENLENVYS 543

Query: 542 LVSDIEM---------AFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQL 543
            V  IE          +FLVA+ NP +Q LE WA ENG+ GDFN++C++ +AK +ILG+L
Sbjct: 544 QVEVIESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDFNSICQNAKAKAFILGEL 603

BLAST of Cp4.1LG13g01110 vs. TAIR 10
Match: AT1G64400.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 718.0 bits (1852), Expect = 5.7e-207
Identity = 361/655 (55.11%), Postives = 448/655 (68.40%), Query Frame = 0

Query: 4   KYLVQVEEGKEAA-AGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNRML 63
           +Y+V+VE+GK+    G PSVGPVYRS++AKDGFP     L + WDIFR+SVEK+P N ML
Sbjct: 5   RYIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRLSVEKSPNNPML 64

Query: 64  GRRKIIEGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWIISM 123
           GRR+I++G   KAG+Y W TY++V+ +V+K+GN++R++G G G+KCGIYGAN PEWIISM
Sbjct: 65  GRREIVDG---KAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEWIISM 124

Query: 124 EACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTLVS 183
           EACNAHGLYCVPLYDTLGAGAIEFIICHAE S+AF EE KI ELLKT P +   LK +VS
Sbjct: 125 EACNAHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISELLKTAPKSTKYLKYIVS 184

Query: 184 FGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPKGV 243
           FG+V+NN + E  +  L IYSW++FL+ G+ + Y+LP K + D+CTIMYTSGTTGDPKGV
Sbjct: 185 FGEVTNNQRVEAERHRLTIYSWDQFLKLGEGKHYELPEKRRSDVCTIMYTSGTTGDPKGV 244

Query: 244 LLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGFWR 303
           LL+N++II L+ GVK LL T+ EE+   DVY+SYLPLAHIFDRVIEE+ I   ASIGFWR
Sbjct: 245 LLTNESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEELCIYEAASIGFWR 304

Query: 304 GDVKLLVEDIGELKPTIFCAVPRVLERIYG------------------------------ 363
           GDVK+L+EDI  LKPT+FCAVPRVLERIY                               
Sbjct: 305 GDVKILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLFNFAFKYKHKNMEK 364

Query: 364 ---------------------------------------------------------GLT 423
                                                                    GLT
Sbjct: 365 GQPHEQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRVVACAHVLQGYGLT 424

Query: 424 ETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLFAG 483
           E+CGGTFV++PNEL MLGTVGPPVPNVDI LESVPEMGYD+LAS PRGE+CI+G TLF+G
Sbjct: 425 ESCGGTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPRGEICIRGKTLFSG 484

Query: 484 YYKREDLTTEVL--------------------------NIFKLSQGEYVAVENLELIYGL 536
           YYKREDLT EV                           NIFKLSQGEYVAVENLE IY  
Sbjct: 485 YYKREDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGEYVAVENLENIYSH 544

BLAST of Cp4.1LG13g01110 vs. TAIR 10
Match: AT1G49430.1 (long-chain acyl-CoA synthetase 2 )

HSP 1 Score: 515.8 bits (1327), Expect = 4.3e-146
Identity = 271/658 (41.19%), Postives = 382/658 (58.05%), Query Frame = 0

Query: 9   VEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNRMLGRRKII 68
           VEEG+ A A  PS GPVYR  +AKDG       +++ W  F  +V+K P  +MLG+R   
Sbjct: 11  VEEGRPATAEHPSAGPVYRCKYAKDGLLDLPTDIDSPWQFFSEAVKKYPNEQMLGQRVTT 70

Query: 69  EGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWIISMEACNAH 128
           +   +K G Y W+TY++ ++  ++IG+A+RS G  PG  CGIYGANCPEWII+MEAC + 
Sbjct: 71  D---SKVGPYTWITYKEAHDAAIRIGSAIRSRGVDPGHCCGIYGANCPEWIIAMEACMSQ 130

Query: 129 GLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTLVSFGKVSN 188
           G+  VPLYD+LG  A+EFII HAE S+ FV+EK +  +L      ++ LKT+VSFG+VS+
Sbjct: 131 GITYVPLYDSLGVNAVEFIINHAEVSLVFVQEKTVSSILSCQKGCSSNLKTIVSFGEVSS 190

Query: 189 NHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPKGVLLSNKA 248
             KEE     + ++SW EF   G+  + +LP K K DICTIMYTSGTTG+PKGV+L+N A
Sbjct: 191 TQKEEAKNQCVSLFSWNEFSLMGNLDEANLPRKRKTDICTIMYTSGTTGEPKGVILNNAA 250

Query: 249 IIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGFWRGDVKLL 308
           I   +  +  +L         +DV+ SYLPLAH +D+V+E  F+  G+S+G+WRGD++ L
Sbjct: 251 ISVQVLSIDKMLEVTDRSCDTSDVFFSYLPLAHCYDQVMEIYFLSRGSSVGYWRGDIRYL 310

Query: 309 VEDIGELKPTIFCAVPRVLERIYG------------------------------------ 368
           ++D+  LKPT+FC VPRV +++Y                                     
Sbjct: 311 MDDVQALKPTVFCGVPRVYDKLYAGIMQKISASGLIRKKLFDFAYNYKLGNMRKGFSQEE 370

Query: 369 ---------------------------------------------------GLTETCGGT 428
                                                              GLTE+CGG+
Sbjct: 371 ASPRLDRLMFDKIKEALGGRAHMLLSGAAPLPRHVEEFLRIIPASNLSQGYGLTESCGGS 430

Query: 429 FVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSL-ASTPRGEVCIKGDTLFAGYYKRE 488
           F TL     M+GTVG P+P V+  L SVPEMGYD+  A  PRGE+C++G+++F+GY+KR+
Sbjct: 431 FTTLAGVFSMVGTVGVPMPTVEARLVSVPEMGYDAFSADVPRGEICLRGNSMFSGYHKRQ 490

Query: 489 DLTTEVL--------------------------NIFKLSQGEYVAVENLELIYG---LVS 543
           DLT +VL                          NIFKLSQGEYVAVENLE  Y    L++
Sbjct: 491 DLTDQVLIDGWFHTGDIGEWQEDGSMKIIDRKKNIFKLSQGEYVAVENLENTYSRCPLIA 550

BLAST of Cp4.1LG13g01110 vs. TAIR 10
Match: AT2G47240.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 477.2 bits (1227), Expect = 1.7e-134
Identity = 261/655 (39.85%), Postives = 365/655 (55.73%), Query Frame = 0

Query: 3   RKYLVQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNRML 62
           + +  +VEEG +   G+PSVGPVYR++ ++ GFPP    + T WDIF  SVEK P N ML
Sbjct: 2   KSFAAKVEEGVKGIDGKPSVGPVYRNLLSEKGFPPIDSEITTAWDIFSKSVEKFPDNNML 61

Query: 63  GRRKIIEGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWIISM 122
           G R+I++    K G Y W TY++VYE VL+IG+A+R+ G  PG + GIYG NCP+WII+M
Sbjct: 62  GWRRIVD---EKVGPYMWKTYKEVYEEVLQIGSALRAAGAEPGSRVGIYGVNCPQWIIAM 121

Query: 123 EACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTLVS 182
           EAC AH L CVPLYDTLG+GA+++I+ HAE    FV++ KI  LL+     A  LK +VS
Sbjct: 122 EACAAHTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQDTKIKGLLEPDCKCAKRLKAIVS 181

Query: 183 FGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPKGV 242
           F  VS+    + ++ G++ YSW +FL  G  +  D       +ICTIMYTSGT+GDPKGV
Sbjct: 182 FTNVSDELSHKASEIGVKTYSWIDFLHMGREKPEDTNPPKAFNICTIMYTSGTSGDPKGV 241

Query: 243 LLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGFWR 302
           +L+++A+   + G+   +   ++++  +DVY+S+LPLAHI DR+ EE F   GAS+G++ 
Sbjct: 242 VLTHQAVATFVVGMDLYMDQFEDKMTHDDVYLSFLPLAHILDRMNEEYFFRKGASVGYYH 301

Query: 303 GDVKLLVEDIGELKPTIFCAVPRVLERIYG------------------------------ 362
           G++ +L +DI ELKPT    VPRV ERI+                               
Sbjct: 302 GNLNVLRDDIQELKPTYLAGVPRVFERIHEGIQKALQELNPRRRFIFNALYKHKLAWLNR 361

Query: 363 ---------------------------------------------------------GLT 422
                                                                    GLT
Sbjct: 362 GYSHSKASPMADFIAFRKIRDKLGGRIRLLVSGGAPLSPEIEEFLRVTCCCFVVQGYGLT 421

Query: 423 ETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLFAG 482
           ET GGT +  P+E+ MLGTVG P    +I LE V EMGYD L   P GE+CI+G  +F+G
Sbjct: 422 ETLGGTALGFPDEMCMLGTVGIPAVYNEIRLEEVSEMGYDPLGENPAGEICIRGQCMFSG 481

Query: 483 YYKREDLTTEVL--------------------------NIFKLSQGEYVAVENLELIYG- 536
           YYK  +LT EV+                          N+ KLSQGEYVA+E+LE I+G 
Sbjct: 482 YYKNPELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYVALEHLENIFGQ 541

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9T0A05.0e-21656.86Long chain acyl-CoA synthetase 4 OS=Arabidopsis thaliana OX=3702 GN=LACS4 PE=2 S... [more]
Q9T0092.1e-20654.75Long chain acyl-CoA synthetase 5 OS=Arabidopsis thaliana OX=3702 GN=LACS5 PE=2 S... [more]
Q9C7W48.0e-20655.11Long chain acyl-CoA synthetase 3 OS=Arabidopsis thaliana OX=3702 GN=LACS3 PE=2 S... [more]
M4ISH11.3e-15543.40Probable CoA ligase CCL6 OS=Humulus lupulus OX=3486 GN=CCL6 PE=2 SV=1[more]
Q9XIA96.0e-14541.19Long chain acyl-CoA synthetase 2 OS=Arabidopsis thaliana OX=3702 GN=LACS2 PE=2 S... [more]
Match NameE-valueIdentityDescription
XP_023549978.10.081.63long chain acyl-CoA synthetase 4-like [Cucurbita pepo subsp. pepo][more]
KAG6579555.10.080.72Long chain acyl-CoA synthetase 4, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022929138.10.080.72long chain acyl-CoA synthetase 4-like [Cucurbita moschata][more]
XP_022970006.10.080.12long chain acyl-CoA synthetase 4-like [Cucurbita maxima][more]
XP_004143868.10.075.60long chain acyl-CoA synthetase 4 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A6J1ER850.080.72long chain acyl-CoA synthetase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111435... [more]
A0A6J1HZD40.080.12long chain acyl-CoA synthetase 4-like OS=Cucurbita maxima OX=3661 GN=LOC11146902... [more]
A0A5D3C2P40.075.00Long chain acyl-CoA synthetase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3AUH90.075.00long chain acyl-CoA synthetase 4-like OS=Cucumis melo OX=3656 GN=LOC103482821 PE... [more]
A0A5A7TH750.072.13Long chain acyl-CoA synthetase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
Match NameE-valueIdentityDescription
AT4G23850.13.6e-21756.86AMP-dependent synthetase and ligase family protein [more]
AT4G11030.11.5e-20754.75AMP-dependent synthetase and ligase family protein [more]
AT1G64400.15.7e-20755.11AMP-dependent synthetase and ligase family protein [more]
AT1G49430.14.3e-14641.19long-chain acyl-CoA synthetase 2 [more]
AT2G47240.11.7e-13439.85AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 69..330
e-value: 5.5E-57
score: 193.3
coord: 333..408
e-value: 7.5E-13
score: 47.8
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 332..418
e-value: 7.5E-12
score: 46.4
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 39..331
e-value: 7.2E-53
score: 181.6
NoneNo IPR availablePANTHERPTHR43272LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 408..536
NoneNo IPR availablePANTHERPTHR43272:SF81LONG CHAIN ACYL-COA SYNTHETASE 4-LIKEcoord: 408..536
coord: 4..335
NoneNo IPR availablePANTHERPTHR43272LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 4..335
NoneNo IPR availablePANTHERPTHR43272:SF81LONG CHAIN ACYL-COA SYNTHETASE 4-LIKEcoord: 333..407
NoneNo IPR availablePANTHERPTHR43272LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 333..407
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 42..535
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 229..240

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG13g01110.1Cp4.1LG13g01110.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001676 long-chain fatty acid metabolic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016020 membrane
molecular_function GO:0004467 long-chain fatty acid-CoA ligase activity