Cp4.1LG13g00660 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG13g00660
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Protein kinase domain-containing protein
Location	Cp4.1LG13: 680553 .. 682494 (+)
RNA-Seq Expression	Cp4.1LG13g00660
Synteny	Cp4.1LG13g00660

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCAAATTCATTCTTCTCGTTGCTTCGGCCGCCATTTTTGTTCTTCTTCTAATCTCCATCTTCTTCTTCTTCTTCCGCCATAAGCTCTCACTAACAGAGCATCGACACGACGTCGAGAGGCCCTGCAACGACGAACACAACAAAAAGGAACACACGCCGCCGCCATCGCCGCCGTCGTTGGCAACTTTTGAGGGTGGCGAGGATTTGACGATCGACGAGATTCTAGAAGCTCCGGGGGAAGTGATTGGCAAATCCCACTATGGAACTCTGTACAAAGCTTCGTTACAGAGCACCCAATCGACCCGGCTACTTAGATTCCTCCGGCCGGGTGCCGGTGGAGGAGAGTACGACGTCGTTCGGTTTCTGGGTTCGATTAGGCATAGGAATTTAGTGCCGATGCTAGGATTCTACGCCGGCGGGAGGGGTGAGAGGCTTCTGGTTCACCCGTTTTATGGGCAGGGGAATTTGGCGGAGTTGATTAGAGGTAAGTTTGAAAATTTTGGTGTGAGATTTTCTGTTTGTTTCTCGAGAAAGTGGAAGGGAAAATGGAGTTCAATTTGGGGTGGGATGTCGGCAACCAGATTTGTTGTCGGTTTTTGGTGGCTGTCGGCGTTTTTGTCCGTAGGGAAATCAGAGATGGTGACCATTCTGGTTCGTCCAGCGGTGCCTTTTTATTTGTCTCTTCTCCTCCAGGATTTTAAATGTCGGTAGAGGTGGAGAGGTTAACGTGGGATCTCACGATCCACCCACTTAGGACTAAACATTTAGACGCACAGTTCGATGTCTGACTATAATATTATTTGTAATAGTACAAGTCCTCTGCTAGTAGATATTATTCGGCTTATAGAAATGTCTGGTTCTCTCCGGATTTCAAAATCTACCCCTTGGGGCTTAATGTCCTAGCACACTACCCGGTGTCTAGCTATGATATCATTTGTAACAGCTCAAGCTCATCGCTGGTAGATATTCTTTTCTTTGGACTTAACCTATCTCACAATCCACCCTTTGGGGGCACAACGTCCTGACAAACCATCCAATGTATCCAATGTCTGGCTATAATACCATTTGTAATAGACAAAACCCACGACCAGTAGATACTTACTTTCCCTATCTCACAATCCACTTTCTTAGGACCCAACATCCTACACACCGCTCGATGTCTGGATATGATACCATAAGTGACTGTCCAAGTCCACCGCTAATAGATATTAGTTTTGAGTTAAAATAATATATTTTAATCCATTTTTTAGGTTTAATTTACTATATATTTTACTTGAACTATAATTTTTTTTTTAATAAAGATTTTTCATTTTTTCATGTTTTCATGTTGTTTTGCAAATTTATGTATTTTCTCTTCAAGAAATAAAATATTCTACAAATTACCTAAAGTTTTTATTTTCCTAATAATTTCAATTGTTCCACTTGTGATTGAACAGAACGAGAATCAGAGTCAAAAAATTGGGAGGTCATTAACAAAATCTCCCTCGGAATCGCAAGAGCCCTAGATCATCTCCACAACGGATTACAGAAACCAATACCTCATGGAAATCTCAAATCCAAGAACATTCTTCTCGATTCAAACTTTGAGCCGCACGTCTCCGATTTCGGCCTCCATCTCCTCCTCAACGCCGCCGCAACTCGAGAAATGCTCCAAGTTTCCGCCGCCTCCGGCTACAAGGCGCCGGAACTGATTAATATGAGCGACGCTGATGAACGAACAGACGTCTTCAGCTATGGCAAGATCTTACTCGAGTTGCTTTCTGCCAAAGAGACGGGCGGAGACGGCGGCGATTGCGGTGTTTCTGAAGAGCGGACTCTCCGATGCCTTCTTCAGATCGGCGAGGCCTGTTGTTCTTCTTCGCCGGATCTTCGGCCGGATTTCAAGGAAGTGATCGCGAAGATTACAGAAATCGGAAGGATTGAGATGGATTTCAGATGA

mRNA sequence

ATGCTCAAATTCATTCTTCTCGTTGCTTCGGCCGCCATTTTTGTTCTTCTTCTAATCTCCATCTTCTTCTTCTTCTTCCGCCATAAGCTCTCACTAACAGAGCATCGACACGACGTCGAGAGGCCCTGCAACGACGAACACAACAAAAAGGAACACACGCCGCCGCCATCGCCGCCGTCGTTGGCAACTTTTGAGGGTGGCGAGGATTTGACGATCGACGAGATTCTAGAAGCTCCGGGGGAAGTGATTGGCAAATCCCACTATGGAACTCTGTACAAAGCTTCGTTACAGAGCACCCAATCGACCCGGCTACTTAGATTCCTCCGGCCGGGTGCCGGTGGAGGAGAGTACGACGTCGTTCGGTTTCTGGGTTCGATTAGGCATAGGAATTTAGTGCCGATGCTAGGATTCTACGCCGGCGGGAGGGGTGAGAGGCTTCTGGTTCACCCGTTTTATGGGCAGGGGAATTTGGCGGAGTTGATTAGAGAACGAGAATCAGAGTCAAAAAATTGGGAGGTCATTAACAAAATCTCCCTCGGAATCGCAAGAGCCCTAGATCATCTCCACAACGGATTACAGAAACCAATACCTCATGGAAATCTCAAATCCAAGAACATTCTTCTCGATTCAAACTTTGAGCCGCACGTCTCCGATTTCGGCCTCCATCTCCTCCTCAACGCCGCCGCAACTCGAGAAATGCTCCAAGTTTCCGCCGCCTCCGGCTACAAGGCGCCGGAACTGATTAATATGAGCGACGCTGATGAACGAACAGACGTCTTCAGCTATGGCAAGATCTTACTCGAGTTGCTTTCTGCCAAAGAGACGGGCGGAGACGGCGGCGATTGCGGTGTTTCTGAAGAGCGGACTCTCCGATGCCTTCTTCAGATCGGCGAGGCCTGTTGTTCTTCTTCGCCGGATCTTCGGCCGGATTTCAAGGAAGTGATCGCGAAGATTACAGAAATCGGAAGGATTGAGATGGATTTCAGATGA

Coding sequence (CDS)

ATGCTCAAATTCATTCTTCTCGTTGCTTCGGCCGCCATTTTTGTTCTTCTTCTAATCTCCATCTTCTTCTTCTTCTTCCGCCATAAGCTCTCACTAACAGAGCATCGACACGACGTCGAGAGGCCCTGCAACGACGAACACAACAAAAAGGAACACACGCCGCCGCCATCGCCGCCGTCGTTGGCAACTTTTGAGGGTGGCGAGGATTTGACGATCGACGAGATTCTAGAAGCTCCGGGGGAAGTGATTGGCAAATCCCACTATGGAACTCTGTACAAAGCTTCGTTACAGAGCACCCAATCGACCCGGCTACTTAGATTCCTCCGGCCGGGTGCCGGTGGAGGAGAGTACGACGTCGTTCGGTTTCTGGGTTCGATTAGGCATAGGAATTTAGTGCCGATGCTAGGATTCTACGCCGGCGGGAGGGGTGAGAGGCTTCTGGTTCACCCGTTTTATGGGCAGGGGAATTTGGCGGAGTTGATTAGAGAACGAGAATCAGAGTCAAAAAATTGGGAGGTCATTAACAAAATCTCCCTCGGAATCGCAAGAGCCCTAGATCATCTCCACAACGGATTACAGAAACCAATACCTCATGGAAATCTCAAATCCAAGAACATTCTTCTCGATTCAAACTTTGAGCCGCACGTCTCCGATTTCGGCCTCCATCTCCTCCTCAACGCCGCCGCAACTCGAGAAATGCTCCAAGTTTCCGCCGCCTCCGGCTACAAGGCGCCGGAACTGATTAATATGAGCGACGCTGATGAACGAACAGACGTCTTCAGCTATGGCAAGATCTTACTCGAGTTGCTTTCTGCCAAAGAGACGGGCGGAGACGGCGGCGATTGCGGTGTTTCTGAAGAGCGGACTCTCCGATGCCTTCTTCAGATCGGCGAGGCCTGTTGTTCTTCTTCGCCGGATCTTCGGCCGGATTTCAAGGAAGTGATCGCGAAGATTACAGAAATCGGAAGGATTGAGATGGATTTCAGATGA

Protein sequence

MLKFILLVASAAIFVLLLISIFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPPSLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEYDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISLGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSAASGYKAPELINMSDADERTDVFSYGKILLELLSAKETGGDGGDCGVSEERTLRCLLQIGEACCSSSPDLRPDFKEVIAKITEIGRIEMDFR

Homology

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BLAST of Cp4.1LG13g00660 vs. ExPASy Swiss-Prot
Match: P33543 (Putative kinase-like protein TMKL1 OS=Arabidopsis thaliana OX=3702 GN=TMKL1 PE=1 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 2.5e-45
Identity = 121/351 (34.47%), Postives = 184/351 (52.42%), Query Frame = 0


Query: 7   LVASAAIFVLLLISIFFFFFRHKLSLTEHRHDVER-PCNDEHNKKEHTPPPSPPSLATFE 66
           L++ A +   LLI   +   + + S  E   D+E     DE  +KE         L  F+
Sbjct: 308 LMSGAVVVASLLIG--YLQNKKRKSSIESEDDLEEGDEEDEIGEKE----GGEGKLVVFQ 367

Query: 67  GGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGA---GGGEYDVVRF 126
           GGE+LT+D++L A G+V+ K+ YGT+YKA L S      LR LR G          V+R 
Sbjct: 368 GGENLTLDDVLNATGQVMEKTSYGTVYKAKL-SDGGNIALRLLREGTCKDRSSCLPVIRQ 427

Query: 127 LGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERE--SESKNWEVINKISLG 186
           LG IRH NLVP+  FY G RGE+LL++ +    +L +L+ E +    + NW   +KI+LG
Sbjct: 428 LGRIRHENLVPLRAFYQGKRGEKLLIYDYLPNISLHDLLHESKPRKPALNWARRHKIALG 487

Query: 187 IARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSAAS 246
           IAR L +LH G + PI HGN++SKN+L+D  F   +++FGL  ++  A   E++  + + 
Sbjct: 488 IARGLAYLHTGQEVPIIHGNIRSKNVLVDDFFFARLTEFGLDKIMVQAVADEIVSQAKSD 547

Query: 247 GYKAPELINMSDADERTDVFSYGKILLELLSAKETGGDGGD------------CGVSEER 306
           GYKAPEL  M   + R+DV+++G +LLE+L  K+ G  G +              V EE 
Sbjct: 548 GYKAPELHKMKKCNPRSDVYAFGILLLEILMGKKPGKSGRNGNEFVDLPSLVKAAVLEET 607

Query: 307 TLRCL-------------------LQIGEACCSSSPDLRPDFKEVIAKITE 321
           T+                      L++   CC+    +RP  +EV+ ++ E
Sbjct: 608 TMEVFDLEAMKGIRSPMEEGLVHALKLAMGCCAPVTTVRPSMEEVVKQLEE 651

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BLAST of Cp4.1LG13g00660 vs. ExPASy Swiss-Prot
Match: Q9SCT4 (Probably inactive leucine-rich repeat receptor-like protein kinase IMK2 OS=Arabidopsis thaliana OX=3702 GN=IMK2 PE=1 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 1.9e-37
Identity = 101/290 (34.83%), Postives = 152/290 (52.41%), Query Frame = 0


Query: 61  LATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFL-RPGAGGGEYD- 120
           L  F+G    T D++L A  E++GKS YGT YKA+L+      + R   +   G  E++ 
Sbjct: 521 LVHFDGPFVFTADDLLCATAEIMGKSTYGTAYKATLEDGNEVAVKRLREKTTKGVKEFEG 580

Query: 121 VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESK-NWEVINKI 180
            V  LG IRH+NL+ +  +Y G +GE+LLV  +  +G+L+  +  R  E+   WE   KI
Sbjct: 581 EVTALGKIRHQNLLALRAYYLGPKGEKLLVFDYMSKGSLSAFLHARGPETLIPWETRMKI 640

Query: 181 SLGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVS 240
           + GI+R L HLH+   + + H NL + NILLD     H++D+GL  L+ AAA   ++  +
Sbjct: 641 AKGISRGLAHLHS--NENMIHENLTASNILLDEQTNAHIADYGLSRLMTAAAATNVIATA 700

Query: 241 AASGYKAPELINMSDADERTDVFSYGKILLELLSAKETG--GDGGDCG------VSEERT 300
              GY+APE   + +A  +TDV+S G I+LELL+ K  G   +G D        V EE T
Sbjct: 701 GTLGYRAPEFSKIKNASAKTDVYSLGIIILELLTGKSPGEPTNGMDLPQWVASIVKEEWT 760

Query: 301 ------------------LRCLLQIGEACCSSSPDLRPDFKEVIAKITEI 322
                             L   L++   C   SP  RP+  +V+ ++ EI
Sbjct: 761 NEVFDLELMRETQSVGDELLNTLKLALHCVDPSPAARPEANQVVEQLEEI 808

[Close]

BLAST of Cp4.1LG13g00660 vs. ExPASy Swiss-Prot
Match: Q9LVI6 (Probable inactive receptor kinase RLK902 OS=Arabidopsis thaliana OX=3702 GN=RLK902 PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 4.1e-35
Identity = 96/286 (33.57%), Postives = 150/286 (52.45%), Query Frame = 0


Query: 72  IDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEY-DVVRFLGSIRHRN 131
           ++++L A  EV+GK  +GT YKA L +     + R         E+ + +  +G++ H N
Sbjct: 360 LEDLLRASAEVLGKGTFGTAYKAVLDAVTLVAVKRLKDVTMADREFKEKIEVVGAMDHEN 419

Query: 132 LVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESK---NWEVINKISLGIARALDH 191
           LVP+  +Y  G  E+LLV+ F   G+L+ L+   +   +   NWEV + I+LG AR LD+
Sbjct: 420 LVPLRAYYYSG-DEKLLVYDFMPMGSLSALLHGNKGAGRPPLNWEVRSGIALGAARGLDY 479

Query: 192 LHNGLQKPI-PHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSAASGYKAPE 251
           LH+  Q P+  HGN+KS NILL ++ +  VSDFGL  L++A++T      + A+GY+APE
Sbjct: 480 LHS--QDPLSSHGNVKSSNILLTNSHDARVSDFGLAQLVSASSTTP----NRATGYRAPE 539

Query: 252 LINMSDADERTDVFSYGKILLELLSAKETGG-----DGGDCG------------------ 311
           + +     ++ DV+S+G +LLELL+ K         +G D                    
Sbjct: 540 VTDPRRVSQKADVYSFGVVLLELLTGKAPSNSVMNEEGMDLARWVHSVAREEWRNEVFDS 599

Query: 312 --------VSEERTLRCLLQIGEACCSSSPDLRPDFKEVIAKITEI 322
                   VS E  +  +LQ+G  C    PD RP   EV+ +I E+
Sbjct: 600 ELMSIETVVSVEEEMAEMLQLGIDCTEQHPDKRPVMVEVVRRIQEL 638

[Close]

BLAST of Cp4.1LG13g00660 vs. ExPASy Swiss-Prot
Match: C0LGP9 (Probable leucine-rich repeat receptor-like protein kinase IMK3 OS=Arabidopsis thaliana OX=3702 GN=IMK3 PE=1 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 5.3e-35
Identity = 111/355 (31.27%), Postives = 175/355 (49.30%), Query Frame = 0


Query: 3   KFILLVASAAIFV--LLLISIFFFFFRHKLSLTEHRHDVERP----CNDEHNKKEHTPPP 62
           K I+L+AS A+ +  L+L+ +     R K + T+ +     P       E   +      
Sbjct: 408 KDIILIASGALLIVMLILVCVLCCLLRKKANETKAKGGEAGPGAVAAKTEKGGEAEAGGE 467

Query: 63  SPPSLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGE 122
           +   L  F+G    T D++L A  E++GKS YGT+YKA+L+      + R LR      +
Sbjct: 468 TGGKLVHFDGPMAFTADDLLCATAEIMGKSTYGTVYKATLEDGSQVAVKR-LREKITKSQ 527

Query: 123 YDV---VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESK-NWE 182
            +    +  LG IRH NL+ +  +Y G +GE+L+V  +  +G+LA  +  R  +   NW 
Sbjct: 528 KEFENEINVLGRIRHPNLLALRAYYLGPKGEKLVVFDYMSRGSLATFLHARGPDVHINWP 587

Query: 183 VINKISLGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATRE 242
               +  G+AR L +LH      I HGNL S N+LLD N    +SD+GL  L+ AAA   
Sbjct: 588 TRMSLIKGMARGLFYLHT--HANIIHGNLTSSNVLLDENITAKISDYGLSRLMTAAAGSS 647

Query: 243 MLQVSAASGYKAPELINMSDADERTDVFSYGKILLELLSAKETGG--DGGD------CGV 302
           ++  + A GY+APEL  +  A+ +TDV+S G I+LELL+ K      +G D        V
Sbjct: 648 VIATAGALGYRAPELSKLKKANTKTDVYSLGVIILELLTGKSPSEALNGVDLPQWVATAV 707

Query: 303 SEERT------------------LRCLLQIGEACCSSSPDLRPDFKEVIAKITEI 322
            EE T                  +   L++   C  ++P  RP+ ++V+ ++ EI
Sbjct: 708 KEEWTNEVFDLELLNDVNTMGDEILNTLKLALHCVDATPSTRPEAQQVMTQLGEI 759

[Close]

BLAST of Cp4.1LG13g00660 vs. ExPASy Swiss-Prot
Match: Q9LP77 (Probable inactive receptor kinase At1g48480 OS=Arabidopsis thaliana OX=3702 GN=RKL1 PE=1 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 3.4e-34
Identity = 89/283 (31.45%), Postives = 145/283 (51.24%), Query Frame = 0


Query: 72  IDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEY-DVVRFLGSIRHRN 131
           ++++L A  EV+GK  +GT YKA L +     + R         E+ + +  +G++ H N
Sbjct: 366 LEDLLRASAEVLGKGTFGTAYKAVLDAVTVVAVKRLKDVMMADKEFKEKIELVGAMDHEN 425

Query: 132 LVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESK---NWEVINKISLGIARALDH 191
           LVP+  +Y   R E+LLV+ F   G+L+ L+       +   NW+V ++I++G AR LD+
Sbjct: 426 LVPLRAYYF-SRDEKLLVYDFMPMGSLSALLHGNRGAGRSPLNWDVRSRIAIGAARGLDY 485

Query: 192 LHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSAASGYKAPEL 251
           LH+       HGN+KS NILL  + +  VSDFGL  L+ ++AT      + A+GY+APE+
Sbjct: 486 LHS-QGTSTSHGNIKSSNILLTKSHDAKVSDFGLAQLVGSSATNP----NRATGYRAPEV 545

Query: 252 INMSDADERTDVFSYGKILLELLSAKETGG-----DGGD--------------------- 311
            +     ++ DV+S+G +LLEL++ K         +G D                     
Sbjct: 546 TDPKRVSQKGDVYSFGVVLLELITGKAPSNSVMNEEGVDLPRWVKSVARDEWRREVFDSE 605

Query: 312 ---CGVSEERTLRCLLQIGEACCSSSPDLRPDFKEVIAKITEI 322
                  EE  +  ++Q+G  C S  PD RP+  EV+ K+  +
Sbjct: 606 LLSLATDEEEMMAEMVQLGLECTSQHPDQRPEMSEVVRKMENL 642

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BLAST of Cp4.1LG13g00660 vs. NCBI nr
Match: XP_023520536.1 (putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023520537.1 putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023550283.1 putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023550284.1 putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 651 bits (1680), Expect = 6.34e-235
Identity = 329/329 (100.00%), Postives = 329/329 (100.00%), Query Frame = 0


Query: 1   MLKFILLVASAAIFVLLLISIFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPPS 60
           MLKFILLVASAAIFVLLLISIFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPPS
Sbjct: 48  MLKFILLVASAAIFVLLLISIFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPPS 107

Query: 61  LATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEYDVV 120
           LATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEYDVV
Sbjct: 108 LATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEYDVV 167

Query: 121 RFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISLG 180
           RFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISLG
Sbjct: 168 RFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISLG 227

Query: 181 IARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSAAS 240
           IARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSAAS
Sbjct: 228 IARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSAAS 287

Query: 241 GYKAPELINMSDADERTDVFSYGKILLELLSAKETGGDGGDCGVSEERTLRCLLQIGEAC 300
           GYKAPELINMSDADERTDVFSYGKILLELLSAKETGGDGGDCGVSEERTLRCLLQIGEAC
Sbjct: 288 GYKAPELINMSDADERTDVFSYGKILLELLSAKETGGDGGDCGVSEERTLRCLLQIGEAC 347

Query: 301 CSSSPDLRPDFKEVIAKITEIGRIEMDFR 329
           CSSSPDLRPDFKEVIAKITEIGRIEMDFR
Sbjct: 348 CSSSPDLRPDFKEVIAKITEIGRIEMDFR 376

[Close]

BLAST of Cp4.1LG13g00660 vs. NCBI nr
Match: XP_022929086.1 (putative kinase-like protein TMKL1 [Cucurbita moschata] >XP_022929087.1 putative kinase-like protein TMKL1 [Cucurbita moschata] >XP_022929088.1 putative kinase-like protein TMKL1 [Cucurbita moschata])

HSP 1 Score: 638 bits (1645), Expect = 2.37e-230
Identity = 321/329 (97.57%), Postives = 326/329 (99.09%), Query Frame = 0


Query: 1   MLKFILLVASAAIFVLLLISIFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPPS 60
           MLKF+LLVASAAIFVLLLISIFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPPS
Sbjct: 1   MLKFMLLVASAAIFVLLLISIFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPPS 60

Query: 61  LATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEYDVV 120
           LATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQ+TRLLRFLRPGAGGGEYDVV
Sbjct: 61  LATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQATRLLRFLRPGAGGGEYDVV 120

Query: 121 RFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISLG 180
           RFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISLG
Sbjct: 121 RFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISLG 180

Query: 181 IARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSAAS 240
           IARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREML+VSAAS
Sbjct: 181 IARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSAAS 240

Query: 241 GYKAPELINMSDADERTDVFSYGKILLELLSAKETGGDGGDCGVSEERTLRCLLQIGEAC 300
           GYKAPELINM+DADERTDVFSYGKILLELLSA+ETGGDGGDCG SEERTLRCLLQIGEAC
Sbjct: 241 GYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDGGDCGGSEERTLRCLLQIGEAC 300

Query: 301 CSSSPDLRPDFKEVIAKITEIGRIEMDFR 329
           CSSSPD RPDFKEVIAKI EIGRIEMDFR
Sbjct: 301 CSSSPDRRPDFKEVIAKIAEIGRIEMDFR 329

[Close]

BLAST of Cp4.1LG13g00660 vs. NCBI nr
Match: KAG6579628.1 (putative kinase-like protein TMKL1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 634 bits (1634), Expect = 1.17e-228
Identity = 322/330 (97.58%), Postives = 325/330 (98.48%), Query Frame = 0


Query: 1   MLKFILLVASAAIFVLLLISIFFFFF-RHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPP 60
           MLKFILLVASAAIFVLLLISIFFFFF RHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPP
Sbjct: 1   MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPP 60

Query: 61  SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEYDV 120
           SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQ TQSTRLLRFLRPGAGGGEYDV
Sbjct: 61  SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRPGAGGGEYDV 120

Query: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISL 180
           VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISL
Sbjct: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISL 180

Query: 181 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSAA 240
           GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREML+VSAA
Sbjct: 181 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSAA 240

Query: 241 SGYKAPELINMSDADERTDVFSYGKILLELLSAKETGGDGGDCGVSEERTLRCLLQIGEA 300
           SGYKAPELINM+DADERTDVFSYGKILLELLSA+ETGGDGGDCG SEERTLRCLLQIGEA
Sbjct: 241 SGYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDGGDCGGSEERTLRCLLQIGEA 300

Query: 301 CCSSSPDLRPDFKEVIAKITEIGRIEMDFR 329
           CCSSSPD RPDFKEVIAKI EIGRIEMDFR
Sbjct: 301 CCSSSPDRRPDFKEVIAKIAEIGRIEMDFR 330

[Close]

BLAST of Cp4.1LG13g00660 vs. NCBI nr
Match: KAG7017083.1 (putative kinase-like protein TMKL1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 631 bits (1628), Expect = 9.98e-228
Identity = 321/331 (96.98%), Postives = 324/331 (97.89%), Query Frame = 0


Query: 1   MLKFILLVASAAIFVLLLISIFFFFF--RHKLSLTEHRHDVERPCNDEHNKKEHTPPPSP 60
           MLKFILLVASAAIFVLLLISIFFFFF  RHKLSLTEHRHDVERPCNDEHNKKEHTPPPSP
Sbjct: 1   MLKFILLVASAAIFVLLLISIFFFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSP 60

Query: 61  PSLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEYD 120
           PSL TFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQ TQSTRLLRFLRPGAGGGEYD
Sbjct: 61  PSLETFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRPGAGGGEYD 120

Query: 121 VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKIS 180
           VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKIS
Sbjct: 121 VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKIS 180

Query: 181 LGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSA 240
           LGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREML+VSA
Sbjct: 181 LGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSA 240

Query: 241 ASGYKAPELINMSDADERTDVFSYGKILLELLSAKETGGDGGDCGVSEERTLRCLLQIGE 300
           ASGYKAPELINM+DADERTDVFSYGKILLELLSA+ETGGDGGDCG SEERTLRCLLQIGE
Sbjct: 241 ASGYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDGGDCGGSEERTLRCLLQIGE 300

Query: 301 ACCSSSPDLRPDFKEVIAKITEIGRIEMDFR 329
           ACCSSSPD RPDFKEVIAKI EIGRIEMDFR
Sbjct: 301 ACCSSSPDRRPDFKEVIAKIAEIGRIEMDFR 331

[Close]

BLAST of Cp4.1LG13g00660 vs. NCBI nr
Match: XP_022969804.1 (putative kinase-like protein TMKL1 [Cucurbita maxima])

HSP 1 Score: 616 bits (1589), Expect = 1.14e-221
Identity = 313/328 (95.43%), Postives = 321/328 (97.87%), Query Frame = 0


Query: 1   MLKFILLVASAAIFVLLLISIFFFFF-RHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPP 60
           MLKFILLVASAAIFVLLLISIFFFFF RHKLSLTEHRHDVERPC D++ KKEHTPPPSPP
Sbjct: 1   MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVERPCTDQNKKKEHTPPPSPP 60

Query: 61  SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEYDV 120
           SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEYDV
Sbjct: 61  SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEYDV 120

Query: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISL 180
           VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLA+LIR  ESE+KNWEVINKISL
Sbjct: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLADLIRVGESETKNWEVINKISL 180

Query: 181 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSAA 240
           GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSAA
Sbjct: 181 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSAA 240

Query: 241 SGYKAPELINMSDADERTDVFSYGKILLELLSAKETGGDGGDCGVSEERTLRCLLQIGEA 300
           SGYKAPELINM+DADERTD+FSYGKILLELLSAKETGGDGGDCGVSEERTLRC+LQIGEA
Sbjct: 241 SGYKAPELINMNDADERTDIFSYGKILLELLSAKETGGDGGDCGVSEERTLRCILQIGEA 300

Query: 301 CCSSSPDLRPDFKEVIAKITEIGRIEMD 327
           C SSSPDLRP+FKEVIAKI EIGRIEMD
Sbjct: 301 CSSSSPDLRPNFKEVIAKIAEIGRIEMD 328

[Close]

BLAST of Cp4.1LG13g00660 vs. ExPASy TrEMBL
Match: A0A6J1ELS2 (putative kinase-like protein TMKL1 OS=Cucurbita moschata OX=3662 GN=LOC111435777 PE=4 SV=1)

HSP 1 Score: 638 bits (1645), Expect = 1.15e-230
Identity = 321/329 (97.57%), Postives = 326/329 (99.09%), Query Frame = 0


Query: 1   MLKFILLVASAAIFVLLLISIFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPPS 60
           MLKF+LLVASAAIFVLLLISIFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPPS
Sbjct: 1   MLKFMLLVASAAIFVLLLISIFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPPS 60

Query: 61  LATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEYDVV 120
           LATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQ+TRLLRFLRPGAGGGEYDVV
Sbjct: 61  LATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQATRLLRFLRPGAGGGEYDVV 120

Query: 121 RFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISLG 180
           RFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISLG
Sbjct: 121 RFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISLG 180

Query: 181 IARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSAAS 240
           IARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREML+VSAAS
Sbjct: 181 IARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSAAS 240

Query: 241 GYKAPELINMSDADERTDVFSYGKILLELLSAKETGGDGGDCGVSEERTLRCLLQIGEAC 300
           GYKAPELINM+DADERTDVFSYGKILLELLSA+ETGGDGGDCG SEERTLRCLLQIGEAC
Sbjct: 241 GYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDGGDCGGSEERTLRCLLQIGEAC 300

Query: 301 CSSSPDLRPDFKEVIAKITEIGRIEMDFR 329
           CSSSPD RPDFKEVIAKI EIGRIEMDFR
Sbjct: 301 CSSSPDRRPDFKEVIAKIAEIGRIEMDFR 329

[Close]

BLAST of Cp4.1LG13g00660 vs. ExPASy TrEMBL
Match: A0A6J1I3Q5 (putative kinase-like protein TMKL1 OS=Cucurbita maxima OX=3661 GN=LOC111468894 PE=4 SV=1)

HSP 1 Score: 616 bits (1589), Expect = 5.51e-222
Identity = 313/328 (95.43%), Postives = 321/328 (97.87%), Query Frame = 0


Query: 1   MLKFILLVASAAIFVLLLISIFFFFF-RHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPP 60
           MLKFILLVASAAIFVLLLISIFFFFF RHKLSLTEHRHDVERPC D++ KKEHTPPPSPP
Sbjct: 1   MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVERPCTDQNKKKEHTPPPSPP 60

Query: 61  SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEYDV 120
           SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEYDV
Sbjct: 61  SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEYDV 120

Query: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISL 180
           VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLA+LIR  ESE+KNWEVINKISL
Sbjct: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLADLIRVGESETKNWEVINKISL 180

Query: 181 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSAA 240
           GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSAA
Sbjct: 181 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSAA 240

Query: 241 SGYKAPELINMSDADERTDVFSYGKILLELLSAKETGGDGGDCGVSEERTLRCLLQIGEA 300
           SGYKAPELINM+DADERTD+FSYGKILLELLSAKETGGDGGDCGVSEERTLRC+LQIGEA
Sbjct: 241 SGYKAPELINMNDADERTDIFSYGKILLELLSAKETGGDGGDCGVSEERTLRCILQIGEA 300

Query: 301 CCSSSPDLRPDFKEVIAKITEIGRIEMD 327
           C SSSPDLRP+FKEVIAKI EIGRIEMD
Sbjct: 301 CSSSSPDLRPNFKEVIAKIAEIGRIEMD 328

[Close]

BLAST of Cp4.1LG13g00660 vs. ExPASy TrEMBL
Match: A0A6J1H0H8 (putative kinase-like protein TMKL1 OS=Cucurbita moschata OX=3662 GN=LOC111459294 PE=4 SV=1)

HSP 1 Score: 484 bits (1247), Expect = 5.81e-170
Identity = 255/336 (75.89%), Postives = 278/336 (82.74%), Query Frame = 0


Query: 1   MLKFILLVASAAIFVLLLISIFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPPS 60
           MLK IL+VASA IFVLLL+SIFF   RHKLS T HR+D+E P  D + KK+ T   +   
Sbjct: 1   MLKLILIVASATIFVLLLVSIFFL--RHKLSKTNHRNDIENPSPDNNQKKQDTAAATVEE 60

Query: 61  LATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEYD-V 120
           L TFEGGEDLTIDEILEAPGEVIGK HYGTLYKASLQSTQ T LL FLRP A     D V
Sbjct: 61  LTTFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDV 120

Query: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISL 180
           VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYG+GNL+E I   ESESKNWE+I KISL
Sbjct: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISL 180

Query: 181 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSAA 240
           GIA+ALDHLH+GL KP+PHGNLK KNI LD +FEP+VSDFGLHLLLNAAATRE+LQ S+A
Sbjct: 181 GIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSA 240

Query: 241 SGYKAPELINMSDADERTDVFSYGKILLELLSAKET--------GGDGGDCGVSEERTLR 300
           SGY APEL+ M+DADERTDV+SYGKILLELLSAK+T        GGDGGDC VS+ER L+
Sbjct: 241 SGYNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQ 300

Query: 301 CLLQIGEACCSSSPDLRPDFKEVIAKITEIGRIEMD 327
           CL QIGEACCSSSP  RPDFKEVI KI EIGRIEMD
Sbjct: 301 CLFQIGEACCSSSPIRRPDFKEVIEKIEEIGRIEMD 334

[Close]

BLAST of Cp4.1LG13g00660 vs. ExPASy TrEMBL
Match: A0A6J1K4E4 (putative kinase-like protein TMKL1 OS=Cucurbita maxima OX=3661 GN=LOC111490677 PE=4 SV=1)

HSP 1 Score: 483 bits (1244), Expect = 1.66e-169
Identity = 254/336 (75.60%), Postives = 276/336 (82.14%), Query Frame = 0


Query: 1   MLKFILLVASAAIFVLLLISIFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPPS 60
           MLK IL+VASA IFVLLL+SIFFF  RHKLS + HRHD+E P  D + KK+ T   +   
Sbjct: 1   MLKLILIVASATIFVLLLLSIFFF--RHKLSKSNHRHDIENPSPDNNQKKQDTTAATVEE 60

Query: 61  LATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEYD-V 120
           L TF+GGEDLTIDEILEAPGEVIGK HYGTLYKASLQSTQ   LL FLRP A     D V
Sbjct: 61  LTTFQGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRATLLMFLRPAAVESSVDDV 120

Query: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISL 180
           VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYG+GNL+E I   ESESKNWE+I KISL
Sbjct: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISL 180

Query: 181 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSAA 240
           GIA+ALDHLH+GL KP+PHGNLK  NI LD NFEP+VSDFGLHLLLNAAATRE+LQ S+A
Sbjct: 181 GIAKALDHLHHGLPKPVPHGNLKPMNIFLDPNFEPYVSDFGLHLLLNAAATRELLQASSA 240

Query: 241 SGYKAPELINMSDADERTDVFSYGKILLELLSAKET--------GGDGGDCGVSEERTLR 300
           SGY APEL+ M+DADERTDV+SYGKILLELLSAKET        GGDGGDC VS+ER L+
Sbjct: 241 SGYNAPELVKMTDADERTDVYSYGKILLELLSAKETMNQTAVACGGDGGDCSVSKERNLQ 300

Query: 301 CLLQIGEACCSSSPDLRPDFKEVIAKITEIGRIEMD 327
           CL QIGEACC SSP  RPDFKEVI KI EIGRIEMD
Sbjct: 301 CLFQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD 334

[Close]

BLAST of Cp4.1LG13g00660 vs. ExPASy TrEMBL
Match: A0A5A7TJN4 (Putative kinase-like protein TMKL1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1167G00210 PE=4 SV=1)

HSP 1 Score: 445 bits (1145), Expect = 1.78e-154
Identity = 243/336 (72.32%), Postives = 275/336 (81.85%), Query Frame = 0


Query: 1   MLKFILLVASAAIFVLLLISIFFFFFRHKLSLTEHRHDVERPCN----DEHNKKEHTPPP 60
           MLK IL +ASA +F+LLLI+IF    R KLS     HD+E+       + +NK +HTP P
Sbjct: 1   MLKLILPLASATLFLLLLITIFLL--RLKLSKN---HDIEKSSTTHKTNNNNKTDHTPLP 60

Query: 61  SPPSLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGE 120
            PP L TFEGG+DLTIDEILEAPGEVIGKSHYGTLYKA LQ+TQ+ RLLRFLRP A GGE
Sbjct: 61  PPPELTTFEGGQDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGGE 120

Query: 121 --YDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVI 180
             +D+VRFLGS+RHRNLVP+LGFYAGGRGE+LLVHPFY  GNLAE IR+ + ESKNWEVI
Sbjct: 121 DQHDLVRFLGSVRHRNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFIRD-DGESKNWEVI 180

Query: 181 NKISLGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREML 240
            KI++GIARAL HLH+GLQKPI HGNL SKN+LLD N +PHVSDFGLHLLLN+AATREML
Sbjct: 181 CKIAIGIARALHHLHSGLQKPISHGNLNSKNVLLDQNLDPHVSDFGLHLLLNSAATREML 240

Query: 241 QVSAASGYKAPELINMSDADERTDVFSYGKILLELL-SAKETGGDG----GDCGVSEERT 300
           QVSA+SGYKAPELINM+ ADERTDV+SYGKILLELL SAKE         GD GVS E+ 
Sbjct: 241 QVSASSGYKAPELINMTHADERTDVYSYGKILLELLISAKEPTIRNPVATGDSGVSGEQI 300

Query: 301 LRCLLQIGEACCSSSPDLRPDFKEVIAKITEIGRIE 325
           LRCL+QIGE CC SSP+LRPDF+EVIAKI EI RIE
Sbjct: 301 LRCLVQIGEDCCRSSPNLRPDFREVIAKIEEIRRIE 330

[Close]

BLAST of Cp4.1LG13g00660 vs. TAIR 10
Match: AT5G07620.1 (Protein kinase superfamily protein )

HSP 1 Score: 308.9 bits (790), Expect = 4.8e-84
Identity = 174/360 (48.33%), Postives = 233/360 (64.72%), Query Frame = 0


Query: 1   MLKFILLVASAAIFVLLLISIFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPPS 60
           +L  +LL  S + F++++  I F   +   S    ++DVE   + +HNK+  +       
Sbjct: 5   LLLTLLLGVSLSAFLIVIFFIIFLRRKESSSTESDQYDVE---SLDHNKQGFS--SETEE 64

Query: 61  LATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRP----GAGGGE 120
           L  F+GGEDLTI +IL+APGEVIGKS YGTLYKASLQ +   R+LRFLRP     +   E
Sbjct: 65  LVIFQGGEDLTICDILDAPGEVIGKSSYGTLYKASLQRSGKIRVLRFLRPVCTVRSDSKE 124

Query: 121 YD-VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVIN 180
           ++ ++  LG +RH NLVP+LGFYAG RGE+L+VHPF+G GNL++ IR  + ES+ W  I 
Sbjct: 125 FNGIIETLGFVRHENLVPLLGFYAGNRGEKLMVHPFFGSGNLSDFIRSGDDESRKWINIL 184

Query: 181 KISLGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQ 240
           +I++GI++ALDHLH G+QKPI HGNLKSKN+LL S+FEP +SDFGLHLLLN +A +E+L 
Sbjct: 185 RITIGISKALDHLHTGMQKPIVHGNLKSKNVLLSSSFEPRISDFGLHLLLNLSAGQEILD 244

Query: 241 VSAASGYKAPELINMSDADERTDVFSYGKILLELLSAKE------TGGD----------- 300
           VSAA GYKAPELI M D  + +DV+S G I+LEL+S KE      TG D           
Sbjct: 245 VSAAEGYKAPELIKMKDVSKESDVYSLGVIMLELVSGKEPINENATGDDEFYLPDFMRNA 304

Query: 301 ---------------GGDCGVSEERTLRCLLQIGEACCSSSPDLRPDFKEVIAKITEIGR 324
                          G D  +SEE  L+   Q+  +CCS SP LRP+ K+V+ K+ EIG+
Sbjct: 305 VLDHRLSDLYRPEILGSDDNLSEECVLK-YFQLAMSCCSPSPSLRPNVKQVLRKLEEIGK 358

[Close]

BLAST of Cp4.1LG13g00660 vs. TAIR 10
Match: AT5G61570.2 (Protein kinase superfamily protein )

HSP 1 Score: 291.6 bits (745), Expect = 8.0e-79
Identity = 173/358 (48.32%), Postives = 227/358 (63.41%), Query Frame = 0


Query: 1   MLKFILLVASAAIFVLL--LISIFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPS- 60
           ++K IL V+    F+++  +I IF F  R      E ++DVE P    + K+E +   S 
Sbjct: 6   LVKLILAVSLPTTFLIMIAIIIIFIFCRRTTTETNEVQYDVESP----YEKQEFSDNGSE 65

Query: 61  -PPSLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRP----GA 120
               L  F GGEDLTI +IL+APGEVIGKS YGTLYKA+LQ +   R+LRFLRP     +
Sbjct: 66  TEEELIIFNGGEDLTICDILDAPGEVIGKSSYGTLYKATLQRSGKVRVLRFLRPLCAVNS 125

Query: 121 GGGEYD-VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYG-QGNLAELIRERESESKN 180
              E++ V+  LG +RH NLVP+LGFY G RGE+L++HPF+G  GNL+  I+  + ++  
Sbjct: 126 DSKEFNGVIESLGFVRHDNLVPLLGFYVGNRGEKLMIHPFFGSSGNLSAFIKCGDVDAHK 185

Query: 181 WEVINKISLGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAAT 240
           W  I  I++GIA+ALDHLH G+QKPI HGNLKSKN+LLD +F P VSDFGLHLLLN AA 
Sbjct: 186 WSNILSITIGIAKALDHLHTGMQKPIVHGNLKSKNVLLDKSFRPRVSDFGLHLLLNLAAG 245

Query: 241 REMLQVSAASGYKAPELINMSDADERTDVFSYGKILLELLSAKE------TGG------- 300
           +E+L+ SAA GYKAPELI M +  + +DV+S+G I+LEL+S KE      TG        
Sbjct: 246 QEVLEASAAEGYKAPELIKMKEVSKESDVYSFGVIMLELVSGKEPTNKNPTGSVLDRNRL 305

Query: 301 --------------DGGDCGVSEERTLRCLLQIGEACCSSSPDLRPDFKEVIAKITEI 322
                         DG   GV+EE  L    Q+  +CCS SP LRP FK+V+ K+ EI
Sbjct: 306 SDLYRPEIIRRCLKDGN--GVTEECVLE-YFQLAMSCCSPSPTLRPSFKQVLRKLEEI 356

[Close]

BLAST of Cp4.1LG13g00660 vs. TAIR 10
Match: AT5G61570.1 (Protein kinase superfamily protein )

HSP 1 Score: 287.0 bits (733), Expect = 2.0e-77
Identity = 173/361 (47.92%), Postives = 227/361 (62.88%), Query Frame = 0


Query: 1   MLKFILLVASAAIFVLL--LISIFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPS- 60
           ++K IL V+    F+++  +I IF F  R      E ++DVE P    + K+E +   S 
Sbjct: 6   LVKLILAVSLPTTFLIMIAIIIIFIFCRRTTTETNEVQYDVESP----YEKQEFSDNGSE 65

Query: 61  -PPSLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRP----GA 120
               L  F GGEDLTI +IL+APGEVIGKS YGTLYKA+LQ +   R+LRFLRP     +
Sbjct: 66  TEEELIIFNGGEDLTICDILDAPGEVIGKSSYGTLYKATLQRSGKVRVLRFLRPLCAVNS 125

Query: 121 GGGEYD-VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYG-QGNLAELIR---ERESE 180
              E++ V+  LG +RH NLVP+LGFY G RGE+L++HPF+G  GNL+  I+     + +
Sbjct: 126 DSKEFNGVIESLGFVRHDNLVPLLGFYVGNRGEKLMIHPFFGSSGNLSAFIKFLAGGDVD 185

Query: 181 SKNWEVINKISLGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNA 240
           +  W  I  I++GIA+ALDHLH G+QKPI HGNLKSKN+LLD +F P VSDFGLHLLLN 
Sbjct: 186 AHKWSNILSITIGIAKALDHLHTGMQKPIVHGNLKSKNVLLDKSFRPRVSDFGLHLLLNL 245

Query: 241 AATREMLQVSAASGYKAPELINMSDADERTDVFSYGKILLELLSAKE------TGG---- 300
           AA +E+L+ SAA GYKAPELI M +  + +DV+S+G I+LEL+S KE      TG     
Sbjct: 246 AAGQEVLEASAAEGYKAPELIKMKEVSKESDVYSFGVIMLELVSGKEPTNKNPTGSVLDR 305

Query: 301 -----------------DGGDCGVSEERTLRCLLQIGEACCSSSPDLRPDFKEVIAKITE 322
                            DG   GV+EE  L    Q+  +CCS SP LRP FK+V+ K+ E
Sbjct: 306 NRLSDLYRPEIIRRCLKDGN--GVTEECVLE-YFQLAMSCCSPSPTLRPSFKQVLRKLEE 359

[Close]

BLAST of Cp4.1LG13g00660 vs. TAIR 10
Match: AT3G24660.1 (transmembrane kinase-like 1 )

HSP 1 Score: 184.1 bits (466), Expect = 1.8e-46
Identity = 121/351 (34.47%), Postives = 184/351 (52.42%), Query Frame = 0


Query: 7   LVASAAIFVLLLISIFFFFFRHKLSLTEHRHDVER-PCNDEHNKKEHTPPPSPPSLATFE 66
           L++ A +   LLI   +   + + S  E   D+E     DE  +KE         L  F+
Sbjct: 308 LMSGAVVVASLLIG--YLQNKKRKSSIESEDDLEEGDEEDEIGEKE----GGEGKLVVFQ 367

Query: 67  GGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGA---GGGEYDVVRF 126
           GGE+LT+D++L A G+V+ K+ YGT+YKA L S      LR LR G          V+R 
Sbjct: 368 GGENLTLDDVLNATGQVMEKTSYGTVYKAKL-SDGGNIALRLLREGTCKDRSSCLPVIRQ 427

Query: 127 LGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERE--SESKNWEVINKISLG 186
           LG IRH NLVP+  FY G RGE+LL++ +    +L +L+ E +    + NW   +KI+LG
Sbjct: 428 LGRIRHENLVPLRAFYQGKRGEKLLIYDYLPNISLHDLLHESKPRKPALNWARRHKIALG 487

Query: 187 IARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSAAS 246
           IAR L +LH G + PI HGN++SKN+L+D  F   +++FGL  ++  A   E++  + + 
Sbjct: 488 IARGLAYLHTGQEVPIIHGNIRSKNVLVDDFFFARLTEFGLDKIMVQAVADEIVSQAKSD 547

Query: 247 GYKAPELINMSDADERTDVFSYGKILLELLSAKETGGDGGD------------CGVSEER 306
           GYKAPEL  M   + R+DV+++G +LLE+L  K+ G  G +              V EE 
Sbjct: 548 GYKAPELHKMKKCNPRSDVYAFGILLLEILMGKKPGKSGRNGNEFVDLPSLVKAAVLEET 607

Query: 307 TLRCL-------------------LQIGEACCSSSPDLRPDFKEVIAKITE 321
           T+                      L++   CC+    +RP  +EV+ ++ E
Sbjct: 608 TMEVFDLEAMKGIRSPMEEGLVHALKLAMGCCAPVTTVRPSMEEVVKQLEE 651

[Close]

BLAST of Cp4.1LG13g00660 vs. TAIR 10
Match: AT3G51740.1 (inflorescence meristem receptor-like kinase 2 )

HSP 1 Score: 157.9 bits (398), Expect = 1.4e-38
Identity = 101/290 (34.83%), Postives = 152/290 (52.41%), Query Frame = 0


Query: 61  LATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFL-RPGAGGGEYD- 120
           L  F+G    T D++L A  E++GKS YGT YKA+L+      + R   +   G  E++ 
Sbjct: 521 LVHFDGPFVFTADDLLCATAEIMGKSTYGTAYKATLEDGNEVAVKRLREKTTKGVKEFEG 580

Query: 121 VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESK-NWEVINKI 180
            V  LG IRH+NL+ +  +Y G +GE+LLV  +  +G+L+  +  R  E+   WE   KI
Sbjct: 581 EVTALGKIRHQNLLALRAYYLGPKGEKLLVFDYMSKGSLSAFLHARGPETLIPWETRMKI 640

Query: 181 SLGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVS 240
           + GI+R L HLH+   + + H NL + NILLD     H++D+GL  L+ AAA   ++  +
Sbjct: 641 AKGISRGLAHLHS--NENMIHENLTASNILLDEQTNAHIADYGLSRLMTAAAATNVIATA 700

Query: 241 AASGYKAPELINMSDADERTDVFSYGKILLELLSAKETG--GDGGDCG------VSEERT 300
              GY+APE   + +A  +TDV+S G I+LELL+ K  G   +G D        V EE T
Sbjct: 701 GTLGYRAPEFSKIKNASAKTDVYSLGIIILELLTGKSPGEPTNGMDLPQWVASIVKEEWT 760

Query: 301 ------------------LRCLLQIGEACCSSSPDLRPDFKEVIAKITEI 322
                             L   L++   C   SP  RP+  +V+ ++ EI
Sbjct: 761 NEVFDLELMRETQSVGDELLNTLKLALHCVDPSPAARPEANQVVEQLEEI 808

The following BLAST results are available for this feature:

BLAST of Cp4.1LG13g00660 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P33543	2.5e-45	34.47	Putative kinase-like protein TMKL1 OS=Arabidopsis thaliana OX=3702 GN=TMKL1 PE=1...	[more]
Q9SCT4	1.9e-37	34.83	Probably inactive leucine-rich repeat receptor-like protein kinase IMK2 OS=Arabi...	[more]
Q9LVI6	4.1e-35	33.57	Probable inactive receptor kinase RLK902 OS=Arabidopsis thaliana OX=3702 GN=RLK9...	[more]
C0LGP9	5.3e-35	31.27	Probable leucine-rich repeat receptor-like protein kinase IMK3 OS=Arabidopsis th...	[more]
Q9LP77	3.4e-34	31.45	Probable inactive receptor kinase At1g48480 OS=Arabidopsis thaliana OX=3702 GN=R...	[more]

BLAST of Cp4.1LG13g00660 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023520536.1	6.34e-235	100.00	putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023520537.1 ...	[more]
XP_022929086.1	2.37e-230	97.57	putative kinase-like protein TMKL1 [Cucurbita moschata] >XP_022929087.1 putative...	[more]
KAG6579628.1	1.17e-228	97.58	putative kinase-like protein TMKL1, partial [Cucurbita argyrosperma subsp. soror...	[more]
KAG7017083.1	9.98e-228	96.98	putative kinase-like protein TMKL1, partial [Cucurbita argyrosperma subsp. argyr...	[more]
XP_022969804.1	1.14e-221	95.43	putative kinase-like protein TMKL1 [Cucurbita maxima]	[more]

BLAST of Cp4.1LG13g00660 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ELS2	1.15e-230	97.57	putative kinase-like protein TMKL1 OS=Cucurbita moschata OX=3662 GN=LOC111435777...	[more]
A0A6J1I3Q5	5.51e-222	95.43	putative kinase-like protein TMKL1 OS=Cucurbita maxima OX=3661 GN=LOC111468894 P...	[more]
A0A6J1H0H8	5.81e-170	75.89	putative kinase-like protein TMKL1 OS=Cucurbita moschata OX=3662 GN=LOC111459294...	[more]
A0A6J1K4E4	1.66e-169	75.60	putative kinase-like protein TMKL1 OS=Cucurbita maxima OX=3661 GN=LOC111490677 P...	[more]
A0A5A7TJN4	1.78e-154	72.32	Putative kinase-like protein TMKL1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]

BLAST of Cp4.1LG13g00660 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G07620.1	4.8e-84	48.33	Protein kinase superfamily protein	[more]
AT5G61570.2	8.0e-79	48.32	Protein kinase superfamily protein	[more]
AT5G61570.1	2.0e-77	47.92	Protein kinase superfamily protein	[more]
AT3G24660.1	1.8e-46	34.47	transmembrane kinase-like 1	[more]
AT3G51740.1	1.4e-38	34.83	inflorescence meristem receptor-like kinase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 325..329
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 72..327 e-value: 8.3E-46 score: 158.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 42..62
None	No IPR available	PANTHER	PTHR48008	LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE IMK3-RELATED	coord: 286..322
None	No IPR available	PANTHER	PTHR48008:SF13	PROTEIN KINASE SUPERFAMILY PROTEIN	coord: 286..322
None	No IPR available	PANTHER	PTHR48008	LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE IMK3-RELATED	coord: 4..276
None	No IPR available	PANTHER	PTHR48008:SF13	PROTEIN KINASE SUPERFAMILY PROTEIN	coord: 4..276
IPR001245	Serine-threonine/tyrosine-protein kinase, catalytic domain	PFAM	PF07714	PK_Tyr_Ser-Thr	coord: 81..274 e-value: 3.0E-22 score: 79.2
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 77..329 score: 23.665276
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 80..318

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG13g00660.1	Cp4.1LG13g00660.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity