Cp4.1LG13g00060 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG13g00060
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionthioredoxin-like fold domain-containing protein MRL7L, chloroplastic
LocationCp4.1LG13: 59536 .. 64826 (-)
RNA-Seq ExpressionCp4.1LG13g00060
SyntenyCp4.1LG13g00060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGAATTTTGGGGTTCTGGGTTCTGAGGGGTTTTACAGAGAAACTCGATACCCATTTCAGCTTCGTTTTCAGTTCTTATCCATCTCTCCTATGAACCAACGCTCTTCGCTTCTCCTTCCTTCTTTATACTTCTTCTCCTTCCTTCTTTATACTTACTTCTTCTCCTTCTCAAGTTCTACGCTTCCTTTGTATGCTAACTAAGTTTGTTTGCCGAAGTTGAAGTTCCATTTAGCCATAACCAACTGGAAATCCAGACTTTCATTTACAACTCTGGAATTTCTTTATTGTTGTATCCTGAACCCTATTCCCAAGCAATACTTGAGGTCGTAATCTTGTAGAAATGGCGCTGCAACATACACTACGTTTACCCTTTCTGGTTTTGCCTTCTTCTAATTGCCGATTCCAAGGGGGTGCTTTCGCTTTTGCGCCCACCAATTCGCAACCCAGCGATGCTTTCGCTTCATGGAGGTTGAAGTCTTCTTATCCTTCATCCACCGCTTCGGTGAACTTGTCTTTCCTGGTAATCTCGTTCTCTAATCCTAGTGTGCTTGTCATATGATGTATCTTCGATTTAGATAGCTAAAAGTGTTTTCCAGTTGATTTCAAAACTTCTTTTAAGAAACATCTGAAAGTTGATTCTCCAAAAACTGATTTTAGAGTGATTTTCTGGGAATCGTTTTAAATTCAATTCATGGTAACTTGATATTTGTAGGATTCTTTTTAGACATGGTGCAACTAAACAAACTTGTATTCAAGTCATTTTTAATAGAACGTAAGTAATCATCAAATGATTTTAGAAAAAGTAAAGTTTATGCCGAACTCACCCTTTGGTGCTTGTTTCTTGTTACTTGGGGAAATTTAATGTGAAATGCTTCATGTTTTGATCTGTAGGGTAGTTATTTTAGAAGCAGCCCTCGAAGGGCCTACAAAACTGCTGGATCCCTCTGGTCAGGTGATGGAAAGAAGAAAAAGGGACAAGCAGATACGGACTCCGATGAGGAAGATGATTCTTCTTCAGCAGAGCAGCAAGACCTTGAGCCTATGGATGTAGAAGAGAGACGGGAATGGAGGGAGAAGATTAGGGAAGTGATTGATATGAACCCCGATGTTGAGGAGGAGATGGACATCATGGAAAGGAGAAGGAAGATGCAAAAGCTTCTAGCTGACTATCCTCTTGTTGTGGAGGAGGAAGATCCTAATTGGCCCGAGGATGCTGACGGTTGGGGTTTCAACTTGGGTCAGTTTTTTGATAAGATCACCATCAAAAATAAGAAAAAGGATGATAAAGATGATGATGATGATAAAGATGACACTGATGATGAAGTAGTTTGGCAAGATGACAACTACATTCGTCCAATTAAGGACATAGCAACTGCTGAATGGGAGGAGGCTGTATTTAAGGACATTAGTCCACTAATCATTCTTGTACATAATCGCTACAAAAGGTATGCTCTTTGAAGTCATCTAATTGCCTGCCCTAAGATTATTAACATTAGGATCTTGTGCGGGTTGTTTGTCTTTATGGCCATTGATCTCATTTATCTATATTTTTTCCCGTGGATTCCAAACCTACAATGCAATGCAAGGATCTCTATTCGTAGAGGTTGAAATTATTTTATTCATATTTGCATTTTGTACACCCAAATGGTTGTCCGATGGTGAGAAGTTAGGAGGTCTGCAGATACTCTCTGGGTTCTAGTCTTGAGTTGAAAACTTAATAACTTACAATTGATGGGATGTTTTGGGCGGGCAACATGAGCATAGTGCAAGGAATAGGCTCTTTGATATTTTCCGGTGACTCTACCCATTGTGATTGTAATGTGGTAAGCTCCCCTATATTAACACTCTTGTGCCACTACCAGTACCCAACCAATGTGATTTTCTCTTCAATCACTTTTCGGGTGGTGTGGAGGTTGTGTTTGTTGATCTTACAAAACTTTCCTTGAGACGTTTGGAAGGAGAAAAATAAGAGGGTCAATTGAAGAATATAATTAGAAGGAATTATATGCAGGACTTCGGACCATGACAACTTAGTCTTTTTCCTTTTCTTTAAAAGAAGAAGAGTGCCCTTTGCTAGAGGCCCTCACATTTCTTGGTTAGGAATGATGCGAGGGAAAATTGGGGGTCAGATACGAAATTTTGATATGTATTTTTTAACATTTTATTTTTACTTATGCTTAAACTGTTATTTGATGGCTGTAGTGGTTTAACAATAACATGGATCACAAGATGATCTGTATGTGGCAGAAATTTGCATATTTTGAGAATCCTGTCTTGTATGTATTTACCTTCACTCAGGCCAAAGGAAAATGAAAAAATTCGCAAGGAGCTAGAGAACGCCATCCACATAATATGGAATTGCAATCTGCCTTCGCCAAGAGTAGGCTTCTCCTATTTGTTGACTGTAGTTTACTCATCTCTCTACCTTTAGCAAATGTCATTAGTTTAAACATATTGTTGTTGATTGCAGTGCGTTGCTGTTGATGCTGTTGTTGAACGCAACTTGGTCTCTGCTCTTCAAGTGTCTGCCTTTCCAGAGATTATCTTCACTAAAGCAGGGAAGATTTTATACCGTGAGAAGGGTAAGTGCGAAACCAAGAGAACAGACATTCTGTGATTTCTTTATTTGTTTGGCTCCAAATCTTGTGACTGTAGAATGAAACCTATTTCAATGTATGAGCTGAGGTCCGAGTTGTGGTTTGGTTCAGGATTTGTAAGTGCAGATGAGTTGTCGAAAATAATGGCGTTCTTCTACTATGGAGCAGCTAAGCCACCATGTTTAAGTGACGTTAGGGATTATCAGGAGGCGATCCCATCAGTTTCTGTTGATGCCTGATTATGCCATAGGAGGATAGGCAAAGTATATGCTCTTTTTGGTCTGTTGTAGATGAGGAGTTTCAGCAATCGGTTTGTTGGGGCAATCTTTTTTGTATTGAAAGGCCAATGTTCTTTGTCTTTTAGTCCTGCAACTTCATGAGCTGTTCAAGGCTATTACCGAAATATGAAATGTATCATAGCTTAAGATGACATAGCGACAAGAGCAGGTAAATGAGATCCTTGTATTATTTTTTTAGGTCAATTCGATTGGGTTCTTAATTAACTGCTCCTGCCACTATTTTAACATAGTTTTTTTTATTTTTTTATTTTATTTGTTTCTCTACTACTTCCGTAAGCTGTTTATTCGAATTCCCTCTAATAAATAGGTGCTCTTCTGGATTCAAAATCTTGTTGGCGATGAGTGATGATGCCTTCGGTTAGTGACCATCAACCAACGTCGTTTCCATGGAAAAGGTAATCTTTTTCAGGCATCTCTTAACATTTGCAGACTCTTTCTACGTGCTAATTTGCATACATTGTAACATCTTCTCGAGTCCACCCTTGTATGAAATTTATGAAATTCTTTGATACCGTTGTTGCATCATTTTAATTTAACAATCAGCTAAAATAATCACATGCAGCTTGGCTAAGTTCTAAGTTCGAGGCAGAGAATGAGCACTATCAAAATTTTCGATTTGGTCTTTAAATTTTCAAATTCGTTTTGTTTTGAGCATTCAACTTCCCATGTCTACTTTAATTTCTAAACTTCTAAAAATCAATGATTTTGGCTTGAAGTCGTACTCGTAAATAACAAGTTGCAGTGGTCGAAGTTCTAATGTCTATTATTTGCATTCTATTTACATTAGCAACATTTTGTTGCTTATTATTATTATTTTTTTGATGGTTTATGAACTTTTTAGGATATAGTACAATGTGAAAAATATGAACAGAAATGTGCATAGGTGACCAAAAATTTAATAGGAAAGAGATGAGTAGGTAAAATGTATAGTTTATAATAGAAGAAATTTGATGGGCATCCATATTAGTTAATTTTGGGAAGAAAAGGAACGGTGGAAATTGCTTGGAAAGAGGAGTAGGTGAGTGGGCGATGGATGCCGTGTGTTGTGGGGCGCCTCACAATAGTTGAAGACATCAATTGTTGACTCGTTATTGTGTTTTTTAATTTTAGTTTGACTCCATATATAACTAACCAGTTGTTCTTTAGGAACCCACTGTGGAACCTTCTTTCCTAAGGTAATAATAGATCAGATAGGTCGAGGCTAGGTCGAGGCTAACCAAGGCTTGGACCCATTTCAATCACACCTTGAGATCATTTAGGCTCGGGGAAAGGTGCGTCTTGTGCTAGGTAAGCTCGAGACTAAGGCTAGCCCTATCTTCTCTATGGTCAATAGGGAACGACTCGAGCGTCTGGTCTCATATCCTCTAAGAAAGGAAGAATGGTGGTCTCATACCCTCTAAGAAAGGAAAAATGGTAAATCCATTCTAAGCTTACAACCCTAAACCATTGTGTCTTTGTTACCTTGTGAATCATTTCATCCCCTAAATTACAAATCTATGCATTGAAAAGGAAAAAAAAGCAAGATTATGGAAAGTACGTTAATTAATCCTAAGCTATCAGGGCATGATTGATAGTCGACAAGAAAAACCAATATTACCATTTAAGAAAGTAATAAGGATTGTAGACATGGATGACTGCGAAAAAAAGTTATCAATTTTGCCCAGCAATTCCAATTTGACTCACTTTGCAATGCAATAACGATCAAAGAAGAAGAAGAAAAAAAAAACCTCAAAAGAAGCATAGCTTTGCAATAGAAAAACCTTTAATGGGTTTCAATATCAACTAATTTCCAGAAAGGAGTGAAGATGATTATGCTGTACACATTAAGTTCGATCCCTGAATTGAATCACAAAATCAACAACCAACCCATCAAAATCTCACTGCCCAGTCAAGCACGACAGAATCCTCTGGCTTCTGAACCTAACAAGCTGTGGCCCATGACTGGACTTGGCGGCGGCGGCGGCGGCGGCGGAGGTAGTTCTCCTCCACTCCTCCGGCGTAGATCCGCCGAATGAGTTGTATCTCCGGTGAGCCCTGGTGGCGCCAGGGTCCTCAGATTTGGAAGTGGGGAAGACGTGGGTTGAAGGGGGATCCACGTAGAAATCAGCAATGTTGAAAGATGGAGAAGCCGCTGCTGCTGCTTCAGGGGAAAGTACTGAATATTGGCTTCTGATTCTGGTTTTCTTCATCGCTCTGTAGAACATCGGAATCAAACCCTCCATTTTGCTTTCTTTTGAACCTTTTTAATTGTAGTCTCTTCCAAGAACAGAGGCAGAGCAGAGAGGGGCTTTGGTTTTGGAACTGGAAATGGAAGACTTTTCTTTTCGTTGTTGTGTGTGTTTGGTTGGTTGGGTTTTGGGTATATATTTAATTTGGAATGCGATGGCTGATGGGC

mRNA sequence

TGGAATTTTGGGGTTCTGGGTTCTGAGGGGTTTTACAGAGAAACTCGATACCCATTTCAGCTTCGTTTTCAGTTCTTATCCATCTCTCCTATGAACCAACGCTCTTCGCTTCTCCTTCCTTCTTTATACTTCTTCTCCTTCCTTCTTTATACTTACTTCTTCTCCTTCTCAAGTTCTACGCTTCCTTTGTATGCTAACTAAGTTTGTTTGCCGAAGTTGAAGTTCCATTTAGCCATAACCAACTGGAAATCCAGACTTTCATTTACAACTCTGGAATTTCTTTATTGTTGTATCCTGAACCCTATTCCCAAGCAATACTTGAGGTCGTAATCTTGTAGAAATGGCGCTGCAACATACACTACGTTTACCCTTTCTGGTTTTGCCTTCTTCTAATTGCCGATTCCAAGGGGGTGCTTTCGCTTTTGCGCCCACCAATTCGCAACCCAGCGATGCTTTCGCTTCATGGAGGTTGAAGTCTTCTTATCCTTCATCCACCGCTTCGGTGAACTTGTCTTTCCTGGGTAGTTATTTTAGAAGCAGCCCTCGAAGGGCCTACAAAACTGCTGGATCCCTCTGGTCAGGTGATGGAAAGAAGAAAAAGGGACAAGCAGATACGGACTCCGATGAGGAAGATGATTCTTCTTCAGCAGAGCAGCAAGACCTTGAGCCTATGGATGTAGAAGAGAGACGGGAATGGAGGGAGAAGATTAGGGAAGTGATTGATATGAACCCCGATGTTGAGGAGGAGATGGACATCATGGAAAGGAGAAGGAAGATGCAAAAGCTTCTAGCTGACTATCCTCTTGTTGTGGAGGAGGAAGATCCTAATTGGCCCGAGGATGCTGACGGAATGATGCGAGGGAAAATTGGGGGTCAGATACGAAATTTTGATATAAATTTGCATATTTTGAGAATCCTGTCTTGTATGTATTTACCTTCACTCAGGCCAAAGGAAAATGAAAAAATTCGCAAGGAGCTAGAGAACGCCATCCACATAATATGGAATTGCAATCTGCCTTCGCCAAGATGCGTTGCTGTTGATGCTGTTGTTGAACGCAACTTGGTCTCTGCTCTTCAAGTGTCTGCCTTTCCAGAGATTATCTTCACTAAAGCAGGGAAGATTTTATACCGTGAGAAGGGATTTGTAAGTGCAGATGAGTTGTCGAAAATAATGGCGTTCTTCTACTATGGAGCAGCTAAGCCACCATGTTTAAGTGACGTTAGGGATTATCAGGAGGCGATCCCATCAGTTTCTGTTGATGCCTGATTATGCCATAGGAGGATAGGCAAAGTATATGCTCTTTTTGGTCTGTTGTAGATGAGGAGTTTCAGCAATCGGTTTGTTGGGGCAATCTTTTTTGTATTGAAAGGCCAATGTTCTTTGTCTTTTAGTCCTGCAACTTCATGAGCTGTTCAAGGCTATTACCGAAATATGAAATGTATCATAGCTTAAGATGACATAGCGACAAGAGCAGGTGCTCTTCTGGATTCAAAATCTTGTTGGCGATGAGTGATGATGCCTTCGGTTAGTGACCATCAACCAACGTCGTTTCCATGGAAAAGAAAGGAGTGAAGATGATTATGCTGTACACATTAAGTTCGATCCCTGAATTGAATCACAAAATCAACAACCAACCCATCAAAATCTCACTGCCCAGTCAAGCACGACAGAATCCTCTGGCTTCTGAACCTAACAAGCTGTGGCCCATGACTGGACTTGGCGGCGGCGGCGGCGGCGGCGGAGGTAGTTCTCCTCCACTCCTCCGGCGTAGATCCGCCGAATGAGTTGTATCTCCGGTGAGCCCTGGTGGCGCCAGGGTCCTCAGATTTGGAAGTGGGGAAGACGTGGGTTGAAGGGGGATCCACGTAGAAATCAGCAATGTTGAAAGATGGAGAAGCCGCTGCTGCTGCTTCAGGGGAAAGTACTGAATATTGGCTTCTGATTCTGGTTTTCTTCATCGCTCTGTAGAACATCGGAATCAAACCCTCCATTTTGCTTTCTTTTGAACCTTTTTAATTGTAGTCTCTTCCAAGAACAGAGGCAGAGCAGAGAGGGGCTTTGGTTTTGGAACTGGAAATGGAAGACTTTTCTTTTCGTTGTTGTGTGTGTTTGGTTGGTTGGGTTTTGGGTATATATTTAATTTGGAATGCGATGGCTGATGGGC

Coding sequence (CDS)

ATGGCGCTGCAACATACACTACGTTTACCCTTTCTGGTTTTGCCTTCTTCTAATTGCCGATTCCAAGGGGGTGCTTTCGCTTTTGCGCCCACCAATTCGCAACCCAGCGATGCTTTCGCTTCATGGAGGTTGAAGTCTTCTTATCCTTCATCCACCGCTTCGGTGAACTTGTCTTTCCTGGGTAGTTATTTTAGAAGCAGCCCTCGAAGGGCCTACAAAACTGCTGGATCCCTCTGGTCAGGTGATGGAAAGAAGAAAAAGGGACAAGCAGATACGGACTCCGATGAGGAAGATGATTCTTCTTCAGCAGAGCAGCAAGACCTTGAGCCTATGGATGTAGAAGAGAGACGGGAATGGAGGGAGAAGATTAGGGAAGTGATTGATATGAACCCCGATGTTGAGGAGGAGATGGACATCATGGAAAGGAGAAGGAAGATGCAAAAGCTTCTAGCTGACTATCCTCTTGTTGTGGAGGAGGAAGATCCTAATTGGCCCGAGGATGCTGACGGAATGATGCGAGGGAAAATTGGGGGTCAGATACGAAATTTTGATATAAATTTGCATATTTTGAGAATCCTGTCTTGTATGTATTTACCTTCACTCAGGCCAAAGGAAAATGAAAAAATTCGCAAGGAGCTAGAGAACGCCATCCACATAATATGGAATTGCAATCTGCCTTCGCCAAGATGCGTTGCTGTTGATGCTGTTGTTGAACGCAACTTGGTCTCTGCTCTTCAAGTGTCTGCCTTTCCAGAGATTATCTTCACTAAAGCAGGGAAGATTTTATACCGTGAGAAGGGATTTGTAAGTGCAGATGAGTTGTCGAAAATAATGGCGTTCTTCTACTATGGAGCAGCTAAGCCACCATGTTTAAGTGACGTTAGGGATTATCAGGAGGCGATCCCATCAGTTTCTGTTGATGCCTGA

Protein sequence

MALQHTLRLPFLVLPSSNCRFQGGAFAFAPTNSQPSDAFASWRLKSSYPSSTASVNLSFLGSYFRSSPRRAYKTAGSLWSGDGKKKKGQADTDSDEEDDSSSAEQQDLEPMDVEERREWREKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVVEEEDPNWPEDADGMMRGKIGGQIRNFDINLHILRILSCMYLPSLRPKENEKIRKELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKAGKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA
Homology
BLAST of Cp4.1LG13g00060 vs. ExPASy Swiss-Prot
Match: Q9SKB6 (Thioredoxin-like fold domain-containing protein MRL7L, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MRL7L PE=1 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 4.0e-61
Identity = 145/276 (52.54%), Postives = 174/276 (63.04%), Query Frame = 0

Query: 83  GKK--KKGQADTDSDEEDDSSSAEQ---QDLEPMDVEERREWREKIREVIDMNPDVE--E 142
           GKK  +K + D+DS++E+D    E    ++    D  ERREWR+ IREVID +PD+E  E
Sbjct: 72  GKKVWRKKEEDSDSEDEEDEVKEETFGGKEASLDDPVERREWRKTIREVIDKHPDIEEDE 131

Query: 143 EMDIMERRRKMQKLLADYPLVVEEEDPNWPEDADGM------------------------ 202
           E+D++E+RRKMQKLLADYPLVV EEDPNWPEDADG                         
Sbjct: 132 EIDMVEKRRKMQKLLADYPLVVNEEDPNWPEDADGWGFSFNQFFNKITIKNEKKEEEDDD 191

Query: 203 -------MRGKIGGQIRNF--------------DINLHILRILSCMYLPSLRPKENEKIR 262
                     +I  Q  N+               +   I  ++  ++    RPKENEK R
Sbjct: 192 EDSEGDDSEKEIVWQDDNYIRPIKDLTTAEWEEAVFKDISPLMVLVHNRYKRPKENEKFR 251

Query: 263 KELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKAGKILYREKGFVS 307
           +ELE AI +IWNC LPSPRCVAVDAVVE +LVSAL+VS FPEIIFTKAGKILYREKG  +
Sbjct: 252 EELEKAIQVIWNCGLPSPRCVAVDAVVETDLVSALKVSVFPEIIFTKAGKILYREKGIRT 311

BLAST of Cp4.1LG13g00060 vs. ExPASy Swiss-Prot
Match: Q0DI48 (Thioredoxin-like fold domain-containing protein MRL7L homolog, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=MRL7L PE=2 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 2.4e-37
Identity = 107/267 (40.07%), Postives = 145/267 (54.31%), Query Frame = 0

Query: 88  GQADTDSDEEDDSSSAEQQDLEPMDV--EERREWREKIREVIDMNPDVEEEMDIMERRRK 147
           G+A  D  E +  SS ++ D EPM +  E+RR  R KIRE++D  P+  E  D  ER+ K
Sbjct: 65  GRARDDGSESE--SSDDEDDDEPMQMTDEQRRTLRRKIREMMDRVPETAEITDPAERKAK 124

Query: 148 MQKLLADYPLVVEEEDPNWPEDAD-------GMMRGKI-----------------GGQ-- 207
           M +LL  Y LVVEEEDPNWPED +       G    KI                 G Q  
Sbjct: 125 MLELLTKYQLVVEEEDPNWPEDDEDGHGFSLGQFFDKITIKAEKKNDDDEEDDAKGNQSD 184

Query: 208 ----------------IRNFDINLHILR----ILSCMYLPSLRPKENEKIRKELENAIHI 267
                           ++  D +  +      ++  ++    RP++NE  R +L  AI +
Sbjct: 185 KEIVWEDDNYIKPIRDVKTMDWDDTVFTDFGPLIVLVHNRYKRPQDNENARDQLVKAIEM 244

Query: 268 IWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKAGKILYREKGFVSADELSKIMA 306
            W  NLPSPRCVAVDA  E +LV AL VS FPE++FT AGKI++R+K   SA+E +++MA
Sbjct: 245 FWEYNLPSPRCVAVDACAEPDLVKALNVSGFPEVLFTNAGKIVHRDKVVRSAEEWTRMMA 304

BLAST of Cp4.1LG13g00060 vs. ExPASy Swiss-Prot
Match: Q5QLS7 (Thioredoxin-like fold domain-containing protein MRL7 homolog, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=MRL7 PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 2.1e-17
Identity = 70/210 (33.33%), Postives = 105/210 (50.00%), Query Frame = 0

Query: 120 REKIREVIDMNPDV---EEEMDIMERRRKMQKLLADYPLVVEEEDPNWPEDAD---GMMR 179
           RE+  EV++   D+   +EE+   E +    +   +   VVEEEDP+WP DAD   G+  
Sbjct: 101 RERDPEVLEGKGDILKRKEELSDEEGKEGTGEEEGELKAVVEEEDPSWPLDADIGWGIRA 160

Query: 180 GKIGGQ--IRNFDIN-----------------LHILRILSCMYLPS----------LRPK 239
            +   +  I+N  ++                 ++ L   S  + PS           R  
Sbjct: 161 SEYFDKHSIKNVTVDGVEIDWEREVEEGWVKEINCLEWESFAFHPSPLIVLVFERYNRAA 220

Query: 240 ENEKIRKELENAIHIIWNC--NLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKAGKIL 293
           +N K  +ELE A  + WN    LP PR V VD  +ER+L  ALQV   P+++F +  KIL
Sbjct: 221 DNWKFLQELEKAAKVYWNSKDRLP-PRTVKVDMNIERDLAFALQVKECPQLLFLRGNKIL 280

BLAST of Cp4.1LG13g00060 vs. ExPASy Swiss-Prot
Match: F4JLC1 (Thioredoxin-like fold domain-containing protein MRL7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MRL7 PE=1 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.8e-13
Identity = 67/246 (27.24%), Postives = 115/246 (46.75%), Query Frame = 0

Query: 90  ADTDSDEE------DDSSSAEQQDLEPMDVEERREWREKIREVIDMNPDV---EEEMDIM 149
           A+T+++E+        S    + + + ++   R         + + NP+V   +E+ + +
Sbjct: 78  AETENNEKFPTKVPRKSKRGRRSEADAVEDYVRSSLERTFSTIKEQNPEVFENKEKANFI 137

Query: 150 ERRRKMQKLLADYPLVVEEEDPNWPEDAD-------------GMMRGKIGGQIRNFD--- 209
           + R   ++   +  +VVEEEDP+WP D D               ++  +G      D   
Sbjct: 138 KDRGVDEEEEEEEEMVVEEEDPDWPVDTDVGWGIKASEYFDTHPIKNVVGDDGSEIDWEG 197

Query: 210 -INLHILRILSCMYLPSL----------------RPKENEKIRKELENAIHIIWNC--NL 269
            I+   ++ ++C+   S                 R  +N K  KELE AI + W+    L
Sbjct: 198 EIDDSWVKEINCLEWESFAFHPSPLVVLVFERYKRASDNWKTLKELEKAIKVYWDAKDRL 257

Query: 270 PSPRCVAVDAVVERNLVSALQVSAFPEIIFTKAGKILYREKGFVSADELSKIMAFFYYGA 292
           P PR V +D  +E +L  AL+    P+I+F +  +ILYREK F +ADEL  ++A FYY A
Sbjct: 258 P-PRAVKIDLNIETDLAYALKAKECPQILFLRGNRILYREKDFRTADELVHMIAHFYYKA 317

BLAST of Cp4.1LG13g00060 vs. NCBI nr
Match: XP_023551318.1 (thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023551319.1 thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 513 bits (1322), Expect = 3.72e-181
Identity = 281/351 (80.06%), Postives = 288/351 (82.05%), Query Frame = 0

Query: 1   MALQHTLRLPFLVLPSSNCRFQGGAFAFAPTNSQPSDAFASWRLKSSYPSSTASVNLSFL 60
           MALQHTLRLPFLVLPSSNCRFQGGAFAFAPTNSQPSDAFASWRLKSSYPSSTASVNLSFL
Sbjct: 1   MALQHTLRLPFLVLPSSNCRFQGGAFAFAPTNSQPSDAFASWRLKSSYPSSTASVNLSFL 60

Query: 61  GSYFRSSPRRAYKTAGSLWSGDGKKKKGQADTDSDEEDDSSSAEQQDLEPMDVEERREWR 120
           GSYFRSSPRRAYKTAGSLWSGDGKKKKGQADTDSDEEDDSSSAEQQDLEPMDVEERREWR
Sbjct: 61  GSYFRSSPRRAYKTAGSLWSGDGKKKKGQADTDSDEEDDSSSAEQQDLEPMDVEERREWR 120

Query: 121 EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVVEEEDPNWPEDADG------MMRG 180
           EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVVEEEDPNWPEDADG          
Sbjct: 121 EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVVEEEDPNWPEDADGWGFNLGQFFD 180

Query: 181 KIGGQIRNFD-------------------------------------INLHILRILSCMY 240
           KI  + +  D                                     +   I  ++  ++
Sbjct: 181 KITIKNKKKDDKDDDDDKDDTDDEVVWQDDNYIRPIKDIATAEWEEAVFKDISPLIILVH 240

Query: 241 LPSLRPKENEKIRKELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTK 300
               RPKENEKIRKELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTK
Sbjct: 241 NRYKRPKENEKIRKELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTK 300

Query: 301 AGKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA 308
           AGKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA
Sbjct: 301 AGKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA 351

BLAST of Cp4.1LG13g00060 vs. NCBI nr
Match: XP_022929067.1 (thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Cucurbita moschata] >XP_022929069.1 thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Cucurbita moschata] >XP_022929070.1 thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Cucurbita moschata] >XP_022929071.1 thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Cucurbita moschata])

HSP 1 Score: 503 bits (1295), Expect = 4.62e-177
Identity = 277/350 (79.14%), Postives = 284/350 (81.14%), Query Frame = 0

Query: 1   MALQHTLRLPFLVLPSSNCRFQGGAFAFAPTNSQPSDAFASWRLKSSYPSSTASVNLSFL 60
           MALQHTLRLPFLVLPSSNCRFQGGAFAFA TNSQPSDAFASWRLKSSYPSS ASV LSFL
Sbjct: 1   MALQHTLRLPFLVLPSSNCRFQGGAFAFAATNSQPSDAFASWRLKSSYPSSIASVKLSFL 60

Query: 61  GSYFRSSPRRAYKTAGSLWSGDGKKKKGQADTDSDEEDDSSSAEQQDLEPMDVEERREWR 120
           GSYFRSSPRRAYKTAGSLWSGDGKKKKGQADTDSDEEDDSSSAEQQDLEPMDVEERREWR
Sbjct: 61  GSYFRSSPRRAYKTAGSLWSGDGKKKKGQADTDSDEEDDSSSAEQQDLEPMDVEERREWR 120

Query: 121 EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVVEEEDPNWPEDADG------MMRG 180
           EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVVEEEDPNWPEDADG          
Sbjct: 121 EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVVEEEDPNWPEDADGWGFNLGQFFD 180

Query: 181 KIGGQIRNFD------------------------------------INLHILRILSCMYL 240
           KI  + +  D                                    +   I  ++  ++ 
Sbjct: 181 KITIKNKKKDDKDDDDKDDNDDEVVWQDDNYIRPIKDIATAEWEEAVFKDISPLIILVHN 240

Query: 241 PSLRPKENEKIRKELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKA 300
              RPKENEKIRKELENAIHIIWNCNLPSPRCVAVDAVVE NLVSALQVSAFPEIIFTKA
Sbjct: 241 RYKRPKENEKIRKELENAIHIIWNCNLPSPRCVAVDAVVECNLVSALQVSAFPEIIFTKA 300

Query: 301 GKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA 308
           GKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA
Sbjct: 301 GKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA 350

BLAST of Cp4.1LG13g00060 vs. NCBI nr
Match: XP_022969940.1 (thioredoxin-like fold domain-containing protein MRL7L, chloroplastic isoform X1 [Cucurbita maxima] >XP_022969941.1 thioredoxin-like fold domain-containing protein MRL7L, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 486 bits (1250), Expect = 3.39e-170
Identity = 269/351 (76.64%), Postives = 280/351 (79.77%), Query Frame = 0

Query: 1   MALQHTLRLPFLVLPSSNCRFQGGAFAFAPTNSQPSDAFASWRLKSSYPSSTASVNLSFL 60
           MALQHTLRLPFLVLPSSNCRFQGGAFAFA TNSQPSDAFASWRLKSSYPSS ASV LSFL
Sbjct: 1   MALQHTLRLPFLVLPSSNCRFQGGAFAFAATNSQPSDAFASWRLKSSYPSSVASVKLSFL 60

Query: 61  GSYFRSSPRRAYKTAGSLWSGDGKKKKGQADTDSDEEDDSSSAEQQDLEPMDVEERREWR 120
           GS+ RSS R AYKTAGSLWSGDGKKKKGQAD DSDEEDDSSSA+QQDLE MDVEERREWR
Sbjct: 61  GSFLRSSTRGAYKTAGSLWSGDGKKKKGQADMDSDEEDDSSSAKQQDLELMDVEERREWR 120

Query: 121 EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVVEEEDPNWPEDADG------MMRG 180
           EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLV+EEEDPNWPEDADG          
Sbjct: 121 EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVMEEEDPNWPEDADGWGFNLGQFFD 180

Query: 181 KIGGQIRNFD-------------------------------------INLHILRILSCMY 240
           KI  + +  D                                     +   I  ++  ++
Sbjct: 181 KITIKNKKKDDKDDDDDKDDNDDEVVWQDDNYIRPIKDIATAEWEEAVFKDISPLIILVH 240

Query: 241 LPSLRPKENEKIRKELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTK 300
               RPKENEKIRKELENAIHIIW+CNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTK
Sbjct: 241 NRYKRPKENEKIRKELENAIHIIWSCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTK 300

Query: 301 AGKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA 308
           AGKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA
Sbjct: 301 AGKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA 351

BLAST of Cp4.1LG13g00060 vs. NCBI nr
Match: KAG7017170.1 (Thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 478 bits (1229), Expect = 4.45e-167
Identity = 264/346 (76.30%), Postives = 278/346 (80.35%), Query Frame = 0

Query: 1   MALQHTLRLPFLVLPSSNCRFQGGAFAFAPTNSQPSDAFASWRLKSSYPSSTASVNLSFL 60
           MALQHTLRLPFLVLPSSNCRFQGGAFAFA TNSQPSDAFASWRLKSSYPSS ASV LSFL
Sbjct: 1   MALQHTLRLPFLVLPSSNCRFQGGAFAFAATNSQPSDAFASWRLKSSYPSSIASVKLSFL 60

Query: 61  GSYFRSSPRRAYKTAGSLWSGDGKKKKGQADTDSDEEDDSSSAEQQDLEPMDVEERREWR 120
           GSYFRSSPRRAYKTAGSLWSGDGKKKKGQADTDS EEDDSSSAEQQDLEPMDVEERREWR
Sbjct: 61  GSYFRSSPRRAYKTAGSLWSGDGKKKKGQADTDSAEEDDSSSAEQQDLEPMDVEERREWR 120

Query: 121 EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVV----------------------- 180
           EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPL++                       
Sbjct: 121 EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLILIGPRMLTVGVSTWVTFLIRSPSK 180

Query: 181 -----EEEDPNWPEDADGMMRGKIGGQIRNFD----------INLHILRILSCMYLPSLR 240
                +++D +  +D D  +  +    IR             +   I  ++  ++    R
Sbjct: 181 NKKKDDKDDDDDKDDTDDEVVWQDDNYIRPIKDIATAEWEEAVFKDISPLIILVHNRYKR 240

Query: 241 PKENEKIRKELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKAGKIL 300
           PKENEKIRKELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKAGKIL
Sbjct: 241 PKENEKIRKELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKAGKIL 300

Query: 301 YREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA 308
           YREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA
Sbjct: 301 YREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA 346

BLAST of Cp4.1LG13g00060 vs. NCBI nr
Match: XP_022969942.1 (thioredoxin-like fold domain-containing protein MRL7L, chloroplastic isoform X2 [Cucurbita maxima])

HSP 1 Score: 471 bits (1211), Expect = 2.35e-164
Identity = 264/351 (75.21%), Postives = 275/351 (78.35%), Query Frame = 0

Query: 1   MALQHTLRLPFLVLPSSNCRFQGGAFAFAPTNSQPSDAFASWRLKSSYPSSTASVNLSFL 60
           MALQHTLRLPFLVLPSSNCRFQGGAFAFA TNSQPSDAFASWRLKSSYPSS AS      
Sbjct: 1   MALQHTLRLPFLVLPSSNCRFQGGAFAFAATNSQPSDAFASWRLKSSYPSSVAS------ 60

Query: 61  GSYFRSSPRRAYKTAGSLWSGDGKKKKGQADTDSDEEDDSSSAEQQDLEPMDVEERREWR 120
           GS+ RSS R AYKTAGSLWSGDGKKKKGQAD DSDEEDDSSSA+QQDLE MDVEERREWR
Sbjct: 61  GSFLRSSTRGAYKTAGSLWSGDGKKKKGQADMDSDEEDDSSSAKQQDLELMDVEERREWR 120

Query: 121 EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVVEEEDPNWPEDADG------MMRG 180
           EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLV+EEEDPNWPEDADG          
Sbjct: 121 EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVMEEEDPNWPEDADGWGFNLGQFFD 180

Query: 181 KIGGQIRNFD-------------------------------------INLHILRILSCMY 240
           KI  + +  D                                     +   I  ++  ++
Sbjct: 181 KITIKNKKKDDKDDDDDKDDNDDEVVWQDDNYIRPIKDIATAEWEEAVFKDISPLIILVH 240

Query: 241 LPSLRPKENEKIRKELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTK 300
               RPKENEKIRKELENAIHIIW+CNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTK
Sbjct: 241 NRYKRPKENEKIRKELENAIHIIWSCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTK 300

Query: 301 AGKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA 308
           AGKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA
Sbjct: 301 AGKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA 345

BLAST of Cp4.1LG13g00060 vs. ExPASy TrEMBL
Match: A0A6J1EMP2 (thioredoxin-like fold domain-containing protein MRL7L, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111435768 PE=4 SV=1)

HSP 1 Score: 503 bits (1295), Expect = 2.24e-177
Identity = 277/350 (79.14%), Postives = 284/350 (81.14%), Query Frame = 0

Query: 1   MALQHTLRLPFLVLPSSNCRFQGGAFAFAPTNSQPSDAFASWRLKSSYPSSTASVNLSFL 60
           MALQHTLRLPFLVLPSSNCRFQGGAFAFA TNSQPSDAFASWRLKSSYPSS ASV LSFL
Sbjct: 1   MALQHTLRLPFLVLPSSNCRFQGGAFAFAATNSQPSDAFASWRLKSSYPSSIASVKLSFL 60

Query: 61  GSYFRSSPRRAYKTAGSLWSGDGKKKKGQADTDSDEEDDSSSAEQQDLEPMDVEERREWR 120
           GSYFRSSPRRAYKTAGSLWSGDGKKKKGQADTDSDEEDDSSSAEQQDLEPMDVEERREWR
Sbjct: 61  GSYFRSSPRRAYKTAGSLWSGDGKKKKGQADTDSDEEDDSSSAEQQDLEPMDVEERREWR 120

Query: 121 EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVVEEEDPNWPEDADG------MMRG 180
           EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVVEEEDPNWPEDADG          
Sbjct: 121 EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVVEEEDPNWPEDADGWGFNLGQFFD 180

Query: 181 KIGGQIRNFD------------------------------------INLHILRILSCMYL 240
           KI  + +  D                                    +   I  ++  ++ 
Sbjct: 181 KITIKNKKKDDKDDDDKDDNDDEVVWQDDNYIRPIKDIATAEWEEAVFKDISPLIILVHN 240

Query: 241 PSLRPKENEKIRKELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKA 300
              RPKENEKIRKELENAIHIIWNCNLPSPRCVAVDAVVE NLVSALQVSAFPEIIFTKA
Sbjct: 241 RYKRPKENEKIRKELENAIHIIWNCNLPSPRCVAVDAVVECNLVSALQVSAFPEIIFTKA 300

Query: 301 GKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA 308
           GKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA
Sbjct: 301 GKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA 350

BLAST of Cp4.1LG13g00060 vs. ExPASy TrEMBL
Match: A0A6J1HZ67 (thioredoxin-like fold domain-containing protein MRL7L, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468989 PE=4 SV=1)

HSP 1 Score: 486 bits (1250), Expect = 1.64e-170
Identity = 269/351 (76.64%), Postives = 280/351 (79.77%), Query Frame = 0

Query: 1   MALQHTLRLPFLVLPSSNCRFQGGAFAFAPTNSQPSDAFASWRLKSSYPSSTASVNLSFL 60
           MALQHTLRLPFLVLPSSNCRFQGGAFAFA TNSQPSDAFASWRLKSSYPSS ASV LSFL
Sbjct: 1   MALQHTLRLPFLVLPSSNCRFQGGAFAFAATNSQPSDAFASWRLKSSYPSSVASVKLSFL 60

Query: 61  GSYFRSSPRRAYKTAGSLWSGDGKKKKGQADTDSDEEDDSSSAEQQDLEPMDVEERREWR 120
           GS+ RSS R AYKTAGSLWSGDGKKKKGQAD DSDEEDDSSSA+QQDLE MDVEERREWR
Sbjct: 61  GSFLRSSTRGAYKTAGSLWSGDGKKKKGQADMDSDEEDDSSSAKQQDLELMDVEERREWR 120

Query: 121 EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVVEEEDPNWPEDADG------MMRG 180
           EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLV+EEEDPNWPEDADG          
Sbjct: 121 EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVMEEEDPNWPEDADGWGFNLGQFFD 180

Query: 181 KIGGQIRNFD-------------------------------------INLHILRILSCMY 240
           KI  + +  D                                     +   I  ++  ++
Sbjct: 181 KITIKNKKKDDKDDDDDKDDNDDEVVWQDDNYIRPIKDIATAEWEEAVFKDISPLIILVH 240

Query: 241 LPSLRPKENEKIRKELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTK 300
               RPKENEKIRKELENAIHIIW+CNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTK
Sbjct: 241 NRYKRPKENEKIRKELENAIHIIWSCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTK 300

Query: 301 AGKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA 308
           AGKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA
Sbjct: 301 AGKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA 351

BLAST of Cp4.1LG13g00060 vs. ExPASy TrEMBL
Match: A0A6J1HXQ7 (thioredoxin-like fold domain-containing protein MRL7L, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111468989 PE=4 SV=1)

HSP 1 Score: 471 bits (1211), Expect = 1.14e-164
Identity = 264/351 (75.21%), Postives = 275/351 (78.35%), Query Frame = 0

Query: 1   MALQHTLRLPFLVLPSSNCRFQGGAFAFAPTNSQPSDAFASWRLKSSYPSSTASVNLSFL 60
           MALQHTLRLPFLVLPSSNCRFQGGAFAFA TNSQPSDAFASWRLKSSYPSS AS      
Sbjct: 1   MALQHTLRLPFLVLPSSNCRFQGGAFAFAATNSQPSDAFASWRLKSSYPSSVAS------ 60

Query: 61  GSYFRSSPRRAYKTAGSLWSGDGKKKKGQADTDSDEEDDSSSAEQQDLEPMDVEERREWR 120
           GS+ RSS R AYKTAGSLWSGDGKKKKGQAD DSDEEDDSSSA+QQDLE MDVEERREWR
Sbjct: 61  GSFLRSSTRGAYKTAGSLWSGDGKKKKGQADMDSDEEDDSSSAKQQDLELMDVEERREWR 120

Query: 121 EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVVEEEDPNWPEDADG------MMRG 180
           EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLV+EEEDPNWPEDADG          
Sbjct: 121 EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVMEEEDPNWPEDADGWGFNLGQFFD 180

Query: 181 KIGGQIRNFD-------------------------------------INLHILRILSCMY 240
           KI  + +  D                                     +   I  ++  ++
Sbjct: 181 KITIKNKKKDDKDDDDDKDDNDDEVVWQDDNYIRPIKDIATAEWEEAVFKDISPLIILVH 240

Query: 241 LPSLRPKENEKIRKELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTK 300
               RPKENEKIRKELENAIHIIW+CNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTK
Sbjct: 241 NRYKRPKENEKIRKELENAIHIIWSCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTK 300

Query: 301 AGKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA 308
           AGKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA
Sbjct: 301 AGKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVSVDA 345

BLAST of Cp4.1LG13g00060 vs. ExPASy TrEMBL
Match: A0A5D3BBT5 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1159G00150 PE=4 SV=1)

HSP 1 Score: 395 bits (1016), Expect = 5.44e-135
Identity = 226/347 (65.13%), Postives = 254/347 (73.20%), Query Frame = 0

Query: 1   MALQHTLRLPFLVLPSSNCRFQGGAFAFAPTNSQPSDAFASWRLKSSYPSSTASVNLSFL 60
           MALQHTL L  L LPSSNC+F+GGA  F  T+S  ++  +SW L+SSYPSS + +   F 
Sbjct: 1   MALQHTLGLLSLALPSSNCKFEGGAL-FVSTSSHTTNLLSSWTLESSYPSSISFMKFPFR 60

Query: 61  GSYFRSSPRRAYKTAGSLWSGDGKKKKGQADTDSDEEDDSSSAEQQDLEPMDVEERREWR 120
           GS FRSS  RAY+TAGS+  GDGKKKKGQA  DSDEEDDSSSA+Q D EPMD EER+EWR
Sbjct: 61  GSCFRSSTLRAYRTAGSIKPGDGKKKKGQAGMDSDEEDDSSSAKQLDAEPMDAEERQEWR 120

Query: 121 EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVVEEEDPNWPEDADGM--------- 180
           EKIR+VIDMNP+VEEE+D MER+RKMQKLLA+YPLVVEEEDP+WPEDADG          
Sbjct: 121 EKIRKVIDMNPNVEEEIDNMERKRKMQKLLAEYPLVVEEEDPDWPEDADGWGFNLGQFFD 180

Query: 181 -------------------MRGKIGGQIRNF-----DINLH---------ILRILSCMYL 240
                                 +I  Q  N+     DI +          I  ++  ++ 
Sbjct: 181 KITIKNKKKDNKFDDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHN 240

Query: 241 PSLRPKENEKIRKELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKA 300
              RPKENEK+RKELENAIHIIWNCNLPSPRCVAVDAVVE NLVSALQVSAFPEIIFTKA
Sbjct: 241 RYKRPKENEKVRKELENAIHIIWNCNLPSPRCVAVDAVVECNLVSALQVSAFPEIIFTKA 300

Query: 301 GKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVS 305
           GKILYREKGFVSADELSKIMAFFYYGAAKPPCL+DV DYQE IPSVS
Sbjct: 301 GKILYREKGFVSADELSKIMAFFYYGAAKPPCLNDVGDYQETIPSVS 346

BLAST of Cp4.1LG13g00060 vs. ExPASy TrEMBL
Match: A0A1S3B4P3 (uncharacterized protein LOC103485750 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485750 PE=4 SV=1)

HSP 1 Score: 395 bits (1016), Expect = 5.44e-135
Identity = 226/347 (65.13%), Postives = 254/347 (73.20%), Query Frame = 0

Query: 1   MALQHTLRLPFLVLPSSNCRFQGGAFAFAPTNSQPSDAFASWRLKSSYPSSTASVNLSFL 60
           MALQHTL L  L LPSSNC+F+GGA  F  T+S  ++  +SW L+SSYPSS + +   F 
Sbjct: 1   MALQHTLGLLSLALPSSNCKFEGGAL-FVSTSSHTTNLLSSWTLESSYPSSISFMKFPFR 60

Query: 61  GSYFRSSPRRAYKTAGSLWSGDGKKKKGQADTDSDEEDDSSSAEQQDLEPMDVEERREWR 120
           GS FRSS  RAY+TAGS+  GDGKKKKGQA  DSDEEDDSSSA+Q D EPMD EER+EWR
Sbjct: 61  GSCFRSSTLRAYRTAGSIKPGDGKKKKGQAGMDSDEEDDSSSAKQLDAEPMDAEERQEWR 120

Query: 121 EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVVEEEDPNWPEDADGM--------- 180
           EKIR+VIDMNP+VEEE+D MER+RKMQKLLA+YPLVVEEEDP+WPEDADG          
Sbjct: 121 EKIRKVIDMNPNVEEEIDNMERKRKMQKLLAEYPLVVEEEDPDWPEDADGWGFNLGQFFD 180

Query: 181 -------------------MRGKIGGQIRNF-----DINLH---------ILRILSCMYL 240
                                 +I  Q  N+     DI +          I  ++  ++ 
Sbjct: 181 KITIKNKKKDNKFDDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHN 240

Query: 241 PSLRPKENEKIRKELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKA 300
              RPKENEK+RKELENAIHIIWNCNLPSPRCVAVDAVVE NLVSALQVSAFPEIIFTKA
Sbjct: 241 RYKRPKENEKVRKELENAIHIIWNCNLPSPRCVAVDAVVECNLVSALQVSAFPEIIFTKA 300

Query: 301 GKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVS 305
           GKILYREKGFVSADELSKIMAFFYYGAAKPPCL+DV DYQE IPSVS
Sbjct: 301 GKILYREKGFVSADELSKIMAFFYYGAAKPPCLNDVGDYQETIPSVS 346

BLAST of Cp4.1LG13g00060 vs. TAIR 10
Match: AT2G31840.1 (Thioredoxin superfamily protein )

HSP 1 Score: 236.5 bits (602), Expect = 2.9e-62
Identity = 145/276 (52.54%), Postives = 174/276 (63.04%), Query Frame = 0

Query: 83  GKK--KKGQADTDSDEEDDSSSAEQ---QDLEPMDVEERREWREKIREVIDMNPDVE--E 142
           GKK  +K + D+DS++E+D    E    ++    D  ERREWR+ IREVID +PD+E  E
Sbjct: 72  GKKVWRKKEEDSDSEDEEDEVKEETFGGKEASLDDPVERREWRKTIREVIDKHPDIEEDE 131

Query: 143 EMDIMERRRKMQKLLADYPLVVEEEDPNWPEDADGM------------------------ 202
           E+D++E+RRKMQKLLADYPLVV EEDPNWPEDADG                         
Sbjct: 132 EIDMVEKRRKMQKLLADYPLVVNEEDPNWPEDADGWGFSFNQFFNKITIKNEKKEEEDDD 191

Query: 203 -------MRGKIGGQIRNF--------------DINLHILRILSCMYLPSLRPKENEKIR 262
                     +I  Q  N+               +   I  ++  ++    RPKENEK R
Sbjct: 192 EDSEGDDSEKEIVWQDDNYIRPIKDLTTAEWEEAVFKDISPLMVLVHNRYKRPKENEKFR 251

Query: 263 KELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKAGKILYREKGFVS 307
           +ELE AI +IWNC LPSPRCVAVDAVVE +LVSAL+VS FPEIIFTKAGKILYREKG  +
Sbjct: 252 EELEKAIQVIWNCGLPSPRCVAVDAVVETDLVSALKVSVFPEIIFTKAGKILYREKGIRT 311

BLAST of Cp4.1LG13g00060 vs. TAIR 10
Match: AT4G28590.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 10 growth stages; BEST Arabidopsis thaliana protein match is: Thioredoxin superfamily protein (TAIR:AT2G31840.1); Has 114 Blast hits to 112 proteins in 39 species: Archae - 2; Bacteria - 0; Metazoa - 17; Fungi - 6; Plants - 67; Viruses - 0; Other Eukaryotes - 22 (source: NCBI BLink). )

HSP 1 Score: 78.2 bits (191), Expect = 1.3e-14
Identity = 67/246 (27.24%), Postives = 115/246 (46.75%), Query Frame = 0

Query: 90  ADTDSDEE------DDSSSAEQQDLEPMDVEERREWREKIREVIDMNPDV---EEEMDIM 149
           A+T+++E+        S    + + + ++   R         + + NP+V   +E+ + +
Sbjct: 78  AETENNEKFPTKVPRKSKRGRRSEADAVEDYVRSSLERTFSTIKEQNPEVFENKEKANFI 137

Query: 150 ERRRKMQKLLADYPLVVEEEDPNWPEDAD-------------GMMRGKIGGQIRNFD--- 209
           + R   ++   +  +VVEEEDP+WP D D               ++  +G      D   
Sbjct: 138 KDRGVDEEEEEEEEMVVEEEDPDWPVDTDVGWGIKASEYFDTHPIKNVVGDDGSEIDWEG 197

Query: 210 -INLHILRILSCMYLPSL----------------RPKENEKIRKELENAIHIIWNC--NL 269
            I+   ++ ++C+   S                 R  +N K  KELE AI + W+    L
Sbjct: 198 EIDDSWVKEINCLEWESFAFHPSPLVVLVFERYKRASDNWKTLKELEKAIKVYWDAKDRL 257

Query: 270 PSPRCVAVDAVVERNLVSALQVSAFPEIIFTKAGKILYREKGFVSADELSKIMAFFYYGA 292
           P PR V +D  +E +L  AL+    P+I+F +  +ILYREK F +ADEL  ++A FYY A
Sbjct: 258 P-PRAVKIDLNIETDLAYALKAKECPQILFLRGNRILYREKDFRTADELVHMIAHFYYKA 317

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SKB64.0e-6152.54Thioredoxin-like fold domain-containing protein MRL7L, chloroplastic OS=Arabidop... [more]
Q0DI482.4e-3740.07Thioredoxin-like fold domain-containing protein MRL7L homolog, chloroplastic OS=... [more]
Q5QLS72.1e-1733.33Thioredoxin-like fold domain-containing protein MRL7 homolog, chloroplastic OS=O... [more]
F4JLC11.8e-1327.24Thioredoxin-like fold domain-containing protein MRL7, chloroplastic OS=Arabidops... [more]
Match NameE-valueIdentityDescription
XP_023551318.13.72e-18180.06thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Cucurbita ... [more]
XP_022929067.14.62e-17779.14thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Cucurbita ... [more]
XP_022969940.13.39e-17076.64thioredoxin-like fold domain-containing protein MRL7L, chloroplastic isoform X1 ... [more]
KAG7017170.14.45e-16776.30Thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Cucurbita ... [more]
XP_022969942.12.35e-16475.21thioredoxin-like fold domain-containing protein MRL7L, chloroplastic isoform X2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1EMP22.24e-17779.14thioredoxin-like fold domain-containing protein MRL7L, chloroplastic OS=Cucurbit... [more]
A0A6J1HZ671.64e-17076.64thioredoxin-like fold domain-containing protein MRL7L, chloroplastic isoform X1 ... [more]
A0A6J1HXQ71.14e-16475.21thioredoxin-like fold domain-containing protein MRL7L, chloroplastic isoform X2 ... [more]
A0A5D3BBT55.44e-13565.13Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3B4P35.44e-13565.13uncharacterized protein LOC103485750 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
Match NameE-valueIdentityDescription
AT2G31840.12.9e-6252.54Thioredoxin superfamily protein [more]
AT4G28590.11.3e-1427.24FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 201..279
e-value: 8.1E-6
score: 27.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 74..115
NoneNo IPR availablePANTHERPTHR34669:SF1THIOREDOXIN-LIKE FOLD DOMAIN-CONTAINING PROTEIN MRL7L, CHLOROPLASTICcoord: 1..171
coord: 202..305
IPR044701Thioredoxin-like fold domain-containing protein MRL7/MRL7LPANTHERPTHR34669THIOREDOXIN-LIKE FOLD DOMAIN-CONTAINING PROTEIN MRL7L, CHLOROPLASTICcoord: 1..171
coord: 202..305

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG13g00060.1Cp4.1LG13g00060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0009570 chloroplast stroma