Cp4.1LG12g11680 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG12g11680
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	adenylate isopentenyltransferase 3, chloroplastic-like
Location	Cp4.1LG12: 8479297 .. 8480295 (-)
RNA-Seq Expression	Cp4.1LG12g11680
Synteny	Cp4.1LG12g11680

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATGTGTCCACCATGTTCATGAGCAAAGAGATTATCACTCAACCATTGATAAATCTTCCTCCTAGAAGAATAAACTCTAACCCGTTCTTCAATCTCCCCAAGCTCGGAAAAGAGAAGGTGGTGATCGTGATCGGCGCTACTGGCACGGGTAAGTCTCGGTTGTCGATCGACATTGCTACCCGATACTCGATGGAAGTTATAAACTCTGATAAAATGCAAGTTTACGATGGGCTAGATATAGTAACAAATAAGGTCACGAAGAGAGAACAACGTGGTGTACCGCATCACTTGCTTGGAGTTATTGATCCCTTTTGTGACTTCTCGGCAACTGACTTCTCTGATATGACTATGTTATCTTTGGATTCTATTTGTAGCCGTGGTCGGCTCCCGATTATCGCTGGTGGCTCGAATTCTTATATTGAAACCTTAGTTGACTCGAACTTTCAGTCGAGATATGAATGTTGCTTTCTTTGGGTAGATGTGTCAATACCTGTATTGCAATCCTTTGTATCTGATAGGGTAGACAAAATGGTAGCTAAGGGAATGGTTGACGAGGCTGAAAAATTCTTTGATCCCGCTAGAGATTATTCTCGAGGGATCAAAAGGGCAATTGGGGTTCCCGAATTCGACTCCTATTTTCGATACAAATCGTTTTTAGACGAGGAAACTCGAGAGAAACTCTTACAAGAATCCATATCAAAAGTGAAGGAGCACACGTGCAAGCTAGCATTGAGGCAATTGGGAAAGATCCAACGACTGATAAATATCAAGGAATGGAACATACACAGACTCGATGCTACAGAAGCCTTTACAAAGCGAGGAAAAGAAGCAGAAGAAGCATGGAACAAGCTCGTCACTGCACGAAGCACAACGATTATCGACCAATTTCTTCATCCGAAGATCGTCACCGTCAAAACCCAGAAGAATTTAGCATCCCTCCCCGGCCCCGGCGTGCCACCGAAGAAAAGTGCAGCCATTGCAGCTGCTACTCCTTGA

mRNA sequence

ATGAATGTGTCCACCATGTTCATGAGCAAAGAGATTATCACTCAACCATTGATAAATCTTCCTCCTAGAAGAATAAACTCTAACCCGTTCTTCAATCTCCCCAAGCTCGGAAAAGAGAAGGTGGTGATCGTGATCGGCGCTACTGGCACGGGTAAGTCTCGGTTGTCGATCGACATTGCTACCCGATACTCGATGGAAGTTATAAACTCTGATAAAATGCAAGTTTACGATGGGCTAGATATAGTAACAAATAAGGTCACGAAGAGAGAACAACGTGGTGTACCGCATCACTTGCTTGGAGTTATTGATCCCTTTTGTGACTTCTCGGCAACTGACTTCTCTGATATGACTATGTTATCTTTGGATTCTATTTGTAGCCGTGGTCGGCTCCCGATTATCGCTGGTGGCTCGAATTCTTATATTGAAACCTTAGTTGACTCGAACTTTCAGTCGAGATATGAATGTTGCTTTCTTTGGGTAGATGTGTCAATACCTGTATTGCAATCCTTTGTATCTGATAGGGTAGACAAAATGGTAGCTAAGGGAATGGTTGACGAGGCTGAAAAATTCTTTGATCCCGCTAGAGATTATTCTCGAGGGATCAAAAGGGCAATTGGGGTTCCCGAATTCGACTCCTATTTTCGATACAAATCGTTTTTAGACGAGGAAACTCGAGAGAAACTCTTACAAGAATCCATATCAAAAGTGAAGGAGCACACGTGCAAGCTAGCATTGAGGCAATTGGGAAAGATCCAACGACTGATAAATATCAAGGAATGGAACATACACAGACTCGATGCTACAGAAGCCTTTACAAAGCGAGGAAAAGAAGCAGAAGAAGCATGGAACAAGCTCGTCACTGCACGAAGCACAACGATTATCGACCAATTTCTTCATCCGAAGATCGTCACCGTCAAAACCCAGAAGAATTTAGCATCCCTCCCCGGCCCCGGCGTGCCACCGAAGAAAAGTGCAGCCATTGCAGCTGCTACTCCTTGA

Coding sequence (CDS)

ATGAATGTGTCCACCATGTTCATGAGCAAAGAGATTATCACTCAACCATTGATAAATCTTCCTCCTAGAAGAATAAACTCTAACCCGTTCTTCAATCTCCCCAAGCTCGGAAAAGAGAAGGTGGTGATCGTGATCGGCGCTACTGGCACGGGTAAGTCTCGGTTGTCGATCGACATTGCTACCCGATACTCGATGGAAGTTATAAACTCTGATAAAATGCAAGTTTACGATGGGCTAGATATAGTAACAAATAAGGTCACGAAGAGAGAACAACGTGGTGTACCGCATCACTTGCTTGGAGTTATTGATCCCTTTTGTGACTTCTCGGCAACTGACTTCTCTGATATGACTATGTTATCTTTGGATTCTATTTGTAGCCGTGGTCGGCTCCCGATTATCGCTGGTGGCTCGAATTCTTATATTGAAACCTTAGTTGACTCGAACTTTCAGTCGAGATATGAATGTTGCTTTCTTTGGGTAGATGTGTCAATACCTGTATTGCAATCCTTTGTATCTGATAGGGTAGACAAAATGGTAGCTAAGGGAATGGTTGACGAGGCTGAAAAATTCTTTGATCCCGCTAGAGATTATTCTCGAGGGATCAAAAGGGCAATTGGGGTTCCCGAATTCGACTCCTATTTTCGATACAAATCGTTTTTAGACGAGGAAACTCGAGAGAAACTCTTACAAGAATCCATATCAAAAGTGAAGGAGCACACGTGCAAGCTAGCATTGAGGCAATTGGGAAAGATCCAACGACTGATAAATATCAAGGAATGGAACATACACAGACTCGATGCTACAGAAGCCTTTACAAAGCGAGGAAAAGAAGCAGAAGAAGCATGGAACAAGCTCGTCACTGCACGAAGCACAACGATTATCGACCAATTTCTTCATCCGAAGATCGTCACCGTCAAAACCCAGAAGAATTTAGCATCCCTCCCCGGCCCCGGCGTGCCACCGAAGAAAAGTGCAGCCATTGCAGCTGCTACTCCTTGA

Protein sequence

MNVSTMFMSKEIITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIATRYSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSATDFSDMTMLSLDSICSRGRLPIIAGGSNSYIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVAKGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSFLDEETREKLLQESISKVKEHTCKLALRQLGKIQRLINIKEWNIHRLDATEAFTKRGKEAEEAWNKLVTARSTTIIDQFLHPKIVTVKTQKNLASLPGPGVPPKKSAAIAAATP

Homology

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BLAST of Cp4.1LG12g11680 vs. ExPASy Swiss-Prot
Match: Q93WC9 (Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT3 PE=1 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 2.1e-87
Identity = 162/304 (53.29%), Postives = 216/304 (71.05%), Query Frame = 0


Query: 1   MNVSTMFMSKEIITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60
           M +S     + +   P ++ PP R   N     P   K+KVV+++GATGTGKSRLS+DIA
Sbjct: 3   MKISMAMCKQPLPPSPTLDFPPARFGPNMLTLNPYGPKDKVVVIMGATGTGKSRLSVDIA 62

Query: 61  TRYSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSATDFSDMTMLS 120
           TR+  E+INSDK+QV+ GLDIVTNK+T  E  GVPHHLLGV+ P  D +A ++  M  LS
Sbjct: 63  TRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMANLS 122

Query: 121 LDSICSRGRLPIIAGGSNSYIETLVD---SNFQSRYECCFLWVDVSIPVLQSFVSDRVDK 180
           ++S+ +RG+LPII GGSNSY+E LVD   + F+SRY+CCFLWVDV++PVL  FVS+RVDK
Sbjct: 123 IESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSERVDK 182

Query: 181 MVAKGMVDEAEKFFDPAR-DYSRGIKRAIGVPEFDSYFRYKSFLDEETREKLLQESISKV 240
           MV  GMV+E  +FFD +  DYSRGIK+AIG PEFD +FR + FL+ E RE+LL + + ++
Sbjct: 183 MVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRFFRNEQFLNVEDREELLSKVLEEI 242

Query: 241 KEHTCKLALRQLGKIQRLINIKEWNIHRLDATEAFTKRGK--EAEEAWNKLVTARSTTII 299
           K +T +LA RQ  KI+RL  +K+W+I R+DAT  FTKR    +A  AW +LV   ST  +
Sbjct: 243 KRNTFELACRQREKIERLRKVKKWSIQRVDATPVFTKRRSKMDANVAWERLVAGPSTDTV 302

[Close]

BLAST of Cp4.1LG12g11680 vs. ExPASy Swiss-Prot
Match: Q94ID2 (Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT5 PE=1 SV=2)

HSP 1 Score: 308.5 bits (789), Expect = 9.0e-83
Identity = 161/275 (58.55%), Postives = 202/275 (73.45%), Query Frame = 0


Query: 29  PFFNLPKLGKEKVVIVIGATGTGKSRLSIDIATRYSMEVINSDKMQVYDGLDIVTNKVTK 88
           PFF      K+KVV V+GATGTGKSRL+ID+ATR+  E++NSDK+QVY GLDIVTNKVT 
Sbjct: 27  PFFR----RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDIVTNKVTP 86

Query: 89  REQRGVPHHLLGVI-DPFCDFSATDFSDMTMLSLDSICSRGRLPIIAGGSNSYIETLVDS 148
            E  GVPHHLLG + D + DF+A DF    + +++SI  R R+PIIAGGSNSYIE LV+ 
Sbjct: 87  EESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALVND 146

Query: 149 --NFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVAKGMVDEAEKFFDP-ARDYSRGIKRA 208
             +F+ RY CCFLWVDVS PVL SFVS+RVDKMV  G+VDE  + FDP + DYS GI+RA
Sbjct: 147 CVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDPSSSDYSAGIRRA 206

Query: 209 IGVPEFDSYFRYK-SFLDEETREKLLQESISKVKEHTCKLALRQLGKIQRLINIKEWNIH 268
           IGVPE D + R +      ET E+LL+ +I K+KE+TC LA RQL KIQRL    +WN+H
Sbjct: 207 IGVPELDEFLRSEMRNYPAETTERLLETAIEKIKENTCLLACRQLQKIQRLYKQWKWNMH 266

Query: 269 RLDATEAFTKRGKEAEEAWNKLVTARSTTIIDQFL 299
           R+DATE F +RG+EA+EAW+  V   S   +++FL
Sbjct: 267 RVDATEVFLRRGEEADEAWDNSVAHPSALAVEKFL 297

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BLAST of Cp4.1LG12g11680 vs. ExPASy Swiss-Prot
Match: Q94ID1 (Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IPT7 PE=2 SV=2)

HSP 1 Score: 276.2 bits (705), Expect = 4.9e-73
Identity = 140/279 (50.18%), Postives = 197/279 (70.61%), Query Frame = 0


Query: 24  RINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIATRYSMEVINSDKMQVYDGLDIVT 83
           ++N N F +     KEKV+ V+GATG+GKSRL+ID+ATR+  E+INSDK+Q+Y GLD++T
Sbjct: 23  KVNVNSFLH----PKEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLT 82

Query: 84  NKVTKREQRGVPHHLLGVIDPFC-DFSATDFSDMTMLSLDSICSRGRLPIIAGGSNSYIE 143
           NKVT +E RGVPHHLLGV D    + +AT +S +   ++  + +  +LPI+AGGSNSYIE
Sbjct: 83  NKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIE 142

Query: 144 TLVDSN---FQSRYECCFLWVDVSIPVLQSFVSDRVDKMVAKGMVDEAEKFFDPARDYSR 203
            LV+ +     + Y+CCF+WVDVS+PVL SFVS RVD+M+  G+++E  + F+P  +YS 
Sbjct: 143 ALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSV 202

Query: 204 GIKRAIGVPEFDSYFRYKSFLDEETREKLLQESISKVKEHTCKLALRQLGKIQRLINIKE 263
           GI+RAIGVPE   Y R +S +D  T+ K+L  ++  +K++T  LA RQL KIQRL    +
Sbjct: 203 GIRRAIGVPELHEYLRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLHKKWK 262

Query: 264 WNIHRLDATEAFTKRG-KEAEEAWNKLVTARSTTIIDQF 298
            ++HR+DATE F KR  +E +EAW  LV   S  I+D+F
Sbjct: 263 MSMHRVDATEVFLKRNVEEQDEAWENLVARPSERIVDKF 297

[Close]

BLAST of Cp4.1LG12g11680 vs. ExPASy Swiss-Prot
Match: Q9LJL4 (Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT8 PE=2 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 5.3e-59
Identity = 131/327 (40.06%), Postives = 196/327 (59.94%), Query Frame = 0


Query: 2   NVSTMFMSKEI--ITQPLINL-PPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSID 61
           N+++ F+S  +  IT P + L PPR +       + +  K+KVV+++GATG+GKS LSID
Sbjct: 3   NLTSTFVSPSMIPITSPRLRLPPPRSVVPMTTVCMEQSYKQKVVVIMGATGSGKSCLSID 62

Query: 62  IATRYSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDP-FCDFSATDFSDMT 121
           +ATR+S E++NSDK+Q YDGL + TN+++  E+ GVPHHLLG + P   + + ++F  + 
Sbjct: 63  LATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHHLLGELPPDDSELTTSEFRSLA 122

Query: 122 MLSLDSICSRGRLPIIAGGSNSYIETLVDSNFQS---------------RYECCFLWVDV 181
             S+  I +RG LPIIAGGSNS+I  L+   F                 RYECCFLWVDV
Sbjct: 123 SRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTYPFSSETSISSGLRYECCFLWVDV 182

Query: 182 SIPVLQSFVSDRVDKMVAKGMVDEAEKFFDPARDYS----RGIKRAIGVPEFDSYF---- 241
           S+ VL  ++S RVD+M+  GM +E   F+DP    S     GI + IG+PEFD YF    
Sbjct: 183 SVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIRAHGIHKTIGIPEFDRYFSLYP 242

Query: 242 ---RYKSFLDEETREKLLQESISKVKEHTCKLALRQLGKIQRLINIKEWNIHRLDATEAF 299
              + K    ++ R+    E++ ++KE+T +LA +Q+ +I +L     W+I RLDAT +F
Sbjct: 243 PERKQKMSEWDQARKGAYDEAVQEIKENTWRLAKKQIERIMKL-KSSGWDIQRLDATPSF 302

[Close]

BLAST of Cp4.1LG12g11680 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 9.0e-59
Identity = 129/300 (43.00%), Postives = 178/300 (59.33%), Query Frame = 0


Query: 38  KEKVVIVIGATGTGKSRLSIDIATRYSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHH 97
           KEK+++++GATGTGKSRLSID+A  + +EVINSDKMQVY GLDI TNK++  ++ GVPHH
Sbjct: 29  KEKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTNKISVPDRGGVPHH 88

Query: 98  LLGVIDPF-CDFSATDFSDMTMLSLDSICSRGRLPIIAGGSNSYIETLVDSNFQS----- 157
           LLG +DP   + +  DF  +   ++  I  R +LP++ GGSNS+I  L+   F S     
Sbjct: 89  LLGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHALLVDRFDSSGPGV 148

Query: 158 -------------RYECCFLWVDVSIPVLQSFVSDRVDKMVAKGMVDEAEKFFDPA-RDY 217
                        RY+CCFLWVDVS+ VL  +++ RVD M+  GM DE  +F+ P   D+
Sbjct: 149 FEEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMFDELAEFYSPEDEDH 208

Query: 218 SR------GIKRAIGVPEFDSYFRYKSFLDEE--------TREKLLQESISKVKEHTCKL 277
                   G+++AIGVPEFD YF      D E         R    +E++  +KE+TC L
Sbjct: 209 DEDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRRGAFEEAVRAIKENTCHL 268

Query: 278 ALRQLGKIQRLINIKEWNIHRLDATEAF-----TKRGKEAEEAWNKLVTARSTTIIDQFL 299
           A RQ+GKI RL     W++ RLDATE+F     +  G++  E W K V   S  I+ +FL
Sbjct: 269 AKRQIGKILRLKG-AGWDLRRLDATESFRAAMTSDSGEKCTEIWEKQVLEPSVKIVSRFL 327

[Close]

BLAST of Cp4.1LG12g11680 vs. NCBI nr
Match: XP_023547390.1 (adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 657 bits (1694), Expect = 1.02e-237
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 0


Query: 1   MNVSTMFMSKEIITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMSKEIITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSKEIITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRYSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSATDFSDMTMLS 120
           TRYSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSATDFSDMTMLS
Sbjct: 61  TRYSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSATDFSDMTMLS 120

Query: 121 LDSICSRGRLPIIAGGSNSYIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVA 180
           LDSICSRGRLPIIAGGSNSYIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVA
Sbjct: 121 LDSICSRGRLPIIAGGSNSYIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVA 180

Query: 181 KGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSFLDEETREKLLQESISKVKEHT 240
           KGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSFLDEETREKLLQESISKVKEHT
Sbjct: 181 KGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSFLDEETREKLLQESISKVKEHT 240

Query: 241 CKLALRQLGKIQRLINIKEWNIHRLDATEAFTKRGKEAEEAWNKLVTARSTTIIDQFLHP 300
           CKLALRQLGKIQRLINIKEWNIHRLDATEAFTKRGKEAEEAWNKLVTARSTTIIDQFLHP
Sbjct: 241 CKLALRQLGKIQRLINIKEWNIHRLDATEAFTKRGKEAEEAWNKLVTARSTTIIDQFLHP 300

Query: 301 KIVTVKTQKNLASLPGPGVPPKKSAAIAAATP 332
           KIVTVKTQKNLASLPGPGVPPKKSAAIAAATP
Sbjct: 301 KIVTVKTQKNLASLPGPGVPPKKSAAIAAATP 332

[Close]

BLAST of Cp4.1LG12g11680 vs. NCBI nr
Match: KAG7014428.1 (Adenylate isopentenyltransferase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 640 bits (1652), Expect = 2.57e-231
Identity = 324/332 (97.59%), Postives = 327/332 (98.49%), Query Frame = 0


Query: 1   MNVSTMFMSKEIITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMSKE+ITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSKEMITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRYSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSATDFSDMTMLS 120
           TR+SMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSA DFSDM MLS
Sbjct: 61  TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMAMLS 120

Query: 121 LDSICSRGRLPIIAGGSNSYIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVA 180
           LDSICSRGRLPIIAGGSNSYIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVA
Sbjct: 121 LDSICSRGRLPIIAGGSNSYIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVA 180

Query: 181 KGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSFLDEETREKLLQESISKVKEHT 240
           KGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSFLDE+TREKLLQESISKVKEHT
Sbjct: 181 KGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSFLDEKTREKLLQESISKVKEHT 240

Query: 241 CKLALRQLGKIQRLINIKEWNIHRLDATEAFTKRGKEAEEAWNKLVTARSTTIIDQFLHP 300
           CKLALRQLGKIQRL NIKEWNIHRLDATE FTKRGKEAEEAWNKLVTARSTTIIDQFLHP
Sbjct: 241 CKLALRQLGKIQRLRNIKEWNIHRLDATEVFTKRGKEAEEAWNKLVTARSTTIIDQFLHP 300

Query: 301 KIVTVKTQKNLASLPGPGVPPKKSAAIAAATP 332
           KIVTVKTQKNLASLPGPGVP KKSAAIAAATP
Sbjct: 301 KIVTVKTQKNLASLPGPGVPLKKSAAIAAATP 332

[Close]

BLAST of Cp4.1LG12g11680 vs. NCBI nr
Match: XP_022954005.1 (adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 630 bits (1626), Expect = 2.36e-227
Identity = 319/332 (96.08%), Postives = 324/332 (97.59%), Query Frame = 0


Query: 1   MNVSTMFMSKEIITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMSKE+ITQPLINLPPRRIN+NPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSKEMITQPLINLPPRRINTNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRYSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSATDFSDMTMLS 120
           TR+SMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSA DFSDM MLS
Sbjct: 61  TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMAMLS 120

Query: 121 LDSICSRGRLPIIAGGSNSYIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVA 180
           LDSICSRGRLPIIAGGSNS+IETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVA
Sbjct: 121 LDSICSRGRLPIIAGGSNSFIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVA 180

Query: 181 KGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSFLDEETREKLLQESISKVKEHT 240
           KGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFR KSFLDE+TREKLLQESISKVKEHT
Sbjct: 181 KGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRCKSFLDEKTREKLLQESISKVKEHT 240

Query: 241 CKLALRQLGKIQRLINIKEWNIHRLDATEAFTKRGKEAEEAWNKLVTARSTTIIDQFLHP 300
           CKLALRQLGKIQRL NIKEWNIHRLDATE FTKRGKEAEEAWNKLVTARSTTIIDQFLHP
Sbjct: 241 CKLALRQLGKIQRLRNIKEWNIHRLDATEVFTKRGKEAEEAWNKLVTARSTTIIDQFLHP 300

Query: 301 KIVTVKTQKNLASLPGPGVPPKKSAAIAAATP 332
           KIVTVKTQKNL SLP P VPPKKSAAIAAATP
Sbjct: 301 KIVTVKTQKNLESLPVPSVPPKKSAAIAAATP 332

[Close]

BLAST of Cp4.1LG12g11680 vs. NCBI nr
Match: XP_022992534.1 (adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 621 bits (1602), Expect = 9.96e-224
Identity = 316/332 (95.18%), Postives = 323/332 (97.29%), Query Frame = 0


Query: 1   MNVSTMFMSKEIITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMSKE+ITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSKEMITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRYSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSATDFSDMTMLS 120
           TR+SMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSA DFSDMTMLS
Sbjct: 61  TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMTMLS 120

Query: 121 LDSICSRGRLPIIAGGSNSYIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVA 180
           LDSI SRGRLPIIAGGSNSYIETLVDSNF+SRYECCF+WVDVSIPVLQSFVS+RVDKMVA
Sbjct: 121 LDSIYSRGRLPIIAGGSNSYIETLVDSNFESRYECCFIWVDVSIPVLQSFVSNRVDKMVA 180

Query: 181 KGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSFLDEETREKLLQESISKVKEHT 240
           KGMVDEAEKFFDP RDYSRGIKRAIGVPEFDSYFRYKSFL+EET+EKLLQESISKVKEHT
Sbjct: 181 KGMVDEAEKFFDPTRDYSRGIKRAIGVPEFDSYFRYKSFLNEETQEKLLQESISKVKEHT 240

Query: 241 CKLALRQLGKIQRLINIKEWNIHRLDATEAFTKRGKEAEEAWNKLVTARSTTIIDQFLHP 300
           CKLALRQLGKIQRL N KEWNIHRLDATEAFTKRGKEAEEAWNKLVT RSTTIIDQFLHP
Sbjct: 241 CKLALRQLGKIQRLRNTKEWNIHRLDATEAFTKRGKEAEEAWNKLVTTRSTTIIDQFLHP 300

Query: 301 KIVTVKTQKNLASLPGPGVPPKKSAAIAAATP 332
           KI TVKTQKNLASLPG  VPPKKSAAIAAATP
Sbjct: 301 KIFTVKTQKNLASLPG--VPPKKSAAIAAATP 330

[Close]

BLAST of Cp4.1LG12g11680 vs. NCBI nr
Match: KAG6575896.1 (Adenylate isopentenyltransferase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 575 bits (1483), Expect = 3.57e-206
Identity = 287/294 (97.62%), Postives = 290/294 (98.64%), Query Frame = 0


Query: 12  IITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIATRYSMEVINSD 71
           +ITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIATR+SMEVINSD
Sbjct: 1   MITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIATRFSMEVINSD 60

Query: 72  KMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSATDFSDMTMLSLDSICSRGRLP 131
           KMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSA DFSDM MLSLDSICSRGRLP
Sbjct: 61  KMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMAMLSLDSICSRGRLP 120

Query: 132 IIAGGSNSYIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVAKGMVDEAEKFF 191
           IIAGGSNSYIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVAKGMVDEAEKFF
Sbjct: 121 IIAGGSNSYIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVAKGMVDEAEKFF 180

Query: 192 DPARDYSRGIKRAIGVPEFDSYFRYKSFLDEETREKLLQESISKVKEHTCKLALRQLGKI 251
           DPARDYSRGIKRAIGVPEFDSYFRYKSFLDE+TREKLLQESISKVKEHTCKLALRQLGKI
Sbjct: 181 DPARDYSRGIKRAIGVPEFDSYFRYKSFLDEKTREKLLQESISKVKEHTCKLALRQLGKI 240

Query: 252 QRLINIKEWNIHRLDATEAFTKRGKEAEEAWNKLVTARSTTIIDQFLHPKIVTV 305
           QRL NIKEWNIHRLDATE FTKRGKEAEEAWNKLVTARSTTIIDQFLHPKIVTV
Sbjct: 241 QRLRNIKEWNIHRLDATEVFTKRGKEAEEAWNKLVTARSTTIIDQFLHPKIVTV 294

[Close]

BLAST of Cp4.1LG12g11680 vs. ExPASy TrEMBL
Match: A0A6J1GR83 (adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111456395 PE=4 SV=1)

HSP 1 Score: 630 bits (1626), Expect = 1.14e-227
Identity = 319/332 (96.08%), Postives = 324/332 (97.59%), Query Frame = 0


Query: 1   MNVSTMFMSKEIITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMSKE+ITQPLINLPPRRIN+NPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSKEMITQPLINLPPRRINTNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRYSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSATDFSDMTMLS 120
           TR+SMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSA DFSDM MLS
Sbjct: 61  TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMAMLS 120

Query: 121 LDSICSRGRLPIIAGGSNSYIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVA 180
           LDSICSRGRLPIIAGGSNS+IETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVA
Sbjct: 121 LDSICSRGRLPIIAGGSNSFIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVA 180

Query: 181 KGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSFLDEETREKLLQESISKVKEHT 240
           KGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFR KSFLDE+TREKLLQESISKVKEHT
Sbjct: 181 KGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRCKSFLDEKTREKLLQESISKVKEHT 240

Query: 241 CKLALRQLGKIQRLINIKEWNIHRLDATEAFTKRGKEAEEAWNKLVTARSTTIIDQFLHP 300
           CKLALRQLGKIQRL NIKEWNIHRLDATE FTKRGKEAEEAWNKLVTARSTTIIDQFLHP
Sbjct: 241 CKLALRQLGKIQRLRNIKEWNIHRLDATEVFTKRGKEAEEAWNKLVTARSTTIIDQFLHP 300

Query: 301 KIVTVKTQKNLASLPGPGVPPKKSAAIAAATP 332
           KIVTVKTQKNL SLP P VPPKKSAAIAAATP
Sbjct: 301 KIVTVKTQKNLESLPVPSVPPKKSAAIAAATP 332

[Close]

BLAST of Cp4.1LG12g11680 vs. ExPASy TrEMBL
Match: A0A6J1JXT3 (adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111488830 PE=4 SV=1)

HSP 1 Score: 621 bits (1602), Expect = 4.82e-224
Identity = 316/332 (95.18%), Postives = 323/332 (97.29%), Query Frame = 0


Query: 1   MNVSTMFMSKEIITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMSKE+ITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSKEMITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRYSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSATDFSDMTMLS 120
           TR+SMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSA DFSDMTMLS
Sbjct: 61  TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMTMLS 120

Query: 121 LDSICSRGRLPIIAGGSNSYIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVA 180
           LDSI SRGRLPIIAGGSNSYIETLVDSNF+SRYECCF+WVDVSIPVLQSFVS+RVDKMVA
Sbjct: 121 LDSIYSRGRLPIIAGGSNSYIETLVDSNFESRYECCFIWVDVSIPVLQSFVSNRVDKMVA 180

Query: 181 KGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSFLDEETREKLLQESISKVKEHT 240
           KGMVDEAEKFFDP RDYSRGIKRAIGVPEFDSYFRYKSFL+EET+EKLLQESISKVKEHT
Sbjct: 181 KGMVDEAEKFFDPTRDYSRGIKRAIGVPEFDSYFRYKSFLNEETQEKLLQESISKVKEHT 240

Query: 241 CKLALRQLGKIQRLINIKEWNIHRLDATEAFTKRGKEAEEAWNKLVTARSTTIIDQFLHP 300
           CKLALRQLGKIQRL N KEWNIHRLDATEAFTKRGKEAEEAWNKLVT RSTTIIDQFLHP
Sbjct: 241 CKLALRQLGKIQRLRNTKEWNIHRLDATEAFTKRGKEAEEAWNKLVTTRSTTIIDQFLHP 300

Query: 301 KIVTVKTQKNLASLPGPGVPPKKSAAIAAATP 332
           KI TVKTQKNLASLPG  VPPKKSAAIAAATP
Sbjct: 301 KIFTVKTQKNLASLPG--VPPKKSAAIAAATP 330

[Close]

BLAST of Cp4.1LG12g11680 vs. ExPASy TrEMBL
Match: A0A6J1KSJ2 (adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111497849 PE=4 SV=1)

HSP 1 Score: 527 bits (1357), Expect = 1.02e-186
Identity = 266/332 (80.12%), Postives = 296/332 (89.16%), Query Frame = 0


Query: 1   MNVSTMFMSKEIITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMS++I  QPLINLPPRRIN+NPFFN+ KL KEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRYSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSATDFSDMTMLS 120
           T++  EVINSDKMQVYDGLDIVTNKVTK+EQRGVPHHLLG+IDPF DFSAT+FSDM MLS
Sbjct: 61  TQFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120

Query: 121 LDSICSRGRLPIIAGGSNSYIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVA 180
           LD+I  R RLPIIAGGSNSYIE L+DSNFQS+YECCFLWVDVS+P+L SFVS RVDKMVA
Sbjct: 121 LDTILGRDRLPIIAGGSNSYIEALIDSNFQSKYECCFLWVDVSMPILHSFVSKRVDKMVA 180

Query: 181 KGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSFLDEETREKLLQESISKVKEHT 240
            GMV+EA++FFDPA DYSRGIKRAIGVPEFDSYFRY+S LD+ET+EKLLQE+ISKVK++T
Sbjct: 181 NGMVNEAKEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKYT 240

Query: 241 CKLALRQLGKIQRLINIKEWNIHRLDATEAFTKRGKEAEEAWNKLVTARSTTIIDQFLHP 300
           CKLA RQ+GKIQRL NIK W IHRLDATEAF K+GKEAEE WNKLVT  STTII+QFLH 
Sbjct: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIEQFLHS 300

Query: 301 KIVTVKTQKNLASLPGPGVPPKKSAAIAAATP 332
            ++T     NLASL G  +P K+SAAIAAATP
Sbjct: 301 NVITTNVPTNLASLLG--LPRKRSAAIAAATP 330

[Close]

BLAST of Cp4.1LG12g11680 vs. ExPASy TrEMBL
Match: A0A6J1H8C9 (adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111460993 PE=4 SV=1)

HSP 1 Score: 523 bits (1346), Expect = 4.82e-185
Identity = 266/332 (80.12%), Postives = 293/332 (88.25%), Query Frame = 0


Query: 1   MNVSTMFMSKEIITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMS++I  QPLINLPPRRIN+NPFFN+ KL KEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRYSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSATDFSDMTMLS 120
           TR+  EVINSDKMQVYDGLDIVTNKVTK+EQRGVPHHLLG+IDPF DFSAT+FSDM MLS
Sbjct: 61  TRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120

Query: 121 LDSICSRGRLPIIAGGSNSYIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVA 180
           L +I  R RLPIIAGGSNSY+E L+DSNFQS+YECCFLWVDVS+PVL SFVS RVDKMVA
Sbjct: 121 LVTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180

Query: 181 KGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSFLDEETREKLLQESISKVKEHT 240
            GMVDEAE+FFDPA DYSRGIKRAIGVPEFDSYFRY+S LD+ET+EKLLQE+ISKVK++T
Sbjct: 181 NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKYT 240

Query: 241 CKLALRQLGKIQRLINIKEWNIHRLDATEAFTKRGKEAEEAWNKLVTARSTTIIDQFLHP 300
           CKLA RQ+GKIQRL NIK W IHRLDATEAF K+GKEAEE WNKLVT  STTIIDQFLH 
Sbjct: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS 300

Query: 301 KIVTVKTQKNLASLPGPGVPPKKSAAIAAATP 332
            ++T K   NLA+L    +   +SAAIAAATP
Sbjct: 301 NVITTKVPTNLATLLD--LSRMRSAAIAAATP 330

[Close]

BLAST of Cp4.1LG12g11680 vs. ExPASy TrEMBL
Match: A0A5D3DBM7 (Adenylate isopentenyltransferase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00080 PE=4 SV=1)

HSP 1 Score: 519 bits (1337), Expect = 1.13e-183
Identity = 267/332 (80.42%), Postives = 292/332 (87.95%), Query Frame = 0


Query: 1   MNVSTMFMSKEIITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60
           MNVS+MFMS+    QPLINLPP R+N+N FFNL KL KEKVV VIGATGTGKSRLSIDIA
Sbjct: 1   MNVSSMFMSEITTQQPLINLPPGRLNTNHFFNLRKLQKEKVVFVIGATGTGKSRLSIDIA 60

Query: 61  TRYSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSATDFSDMTMLS 120
           TR+  EVINSDKMQVY GLDIVTNK+TK+EQ GVPHHLLG+I+PF DFSA++FSDM MLS
Sbjct: 61  TRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHHLLGIINPFLDFSASNFSDMAMLS 120

Query: 121 LDSICSRGRLPIIAGGSNSYIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVA 180
           LD+I S  RLPIIAGGSNSYIE L+DSNF+ +YECCFLWVDVS+ VLQSFVS+RVDKMVA
Sbjct: 121 LDAISSHDRLPIIAGGSNSYIEALIDSNFRLKYECCFLWVDVSMQVLQSFVSERVDKMVA 180

Query: 181 KGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSFLDEETREKLLQESISKVKEHT 240
            GMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFR +S LDEE +EKLL+ESI+KVKEHT
Sbjct: 181 NGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRCRSLLDEEAQEKLLEESIAKVKEHT 240

Query: 241 CKLALRQLGKIQRLINIKEWNIHRLDATEAFTKRGKEAEEAWNKLVTARSTTIIDQFLHP 300
           CKLAL+QLGKIQRL NIK WNIHRLDATEAFTK+GKEAEEAWNKLVT  STTIIDQFLHP
Sbjct: 241 CKLALKQLGKIQRLRNIKGWNIHRLDATEAFTKQGKEAEEAWNKLVTIPSTTIIDQFLHP 300

Query: 301 KIVTVKTQKNLASLPGPGVPPKKSAAIAAATP 332
             V  K   NLASL G  VP K+SAA+AAATP
Sbjct: 301 NAVATKFPTNLASLIG--VPRKRSAAMAAATP 330

[Close]

BLAST of Cp4.1LG12g11680 vs. TAIR 10
Match: AT3G63110.1 (isopentenyltransferase 3 )

HSP 1 Score: 323.9 bits (829), Expect = 1.5e-88
Identity = 162/304 (53.29%), Postives = 216/304 (71.05%), Query Frame = 0


Query: 1   MNVSTMFMSKEIITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60
           M +S     + +   P ++ PP R   N     P   K+KVV+++GATGTGKSRLS+DIA
Sbjct: 3   MKISMAMCKQPLPPSPTLDFPPARFGPNMLTLNPYGPKDKVVVIMGATGTGKSRLSVDIA 62

Query: 61  TRYSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSATDFSDMTMLS 120
           TR+  E+INSDK+QV+ GLDIVTNK+T  E  GVPHHLLGV+ P  D +A ++  M  LS
Sbjct: 63  TRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMANLS 122

Query: 121 LDSICSRGRLPIIAGGSNSYIETLVD---SNFQSRYECCFLWVDVSIPVLQSFVSDRVDK 180
           ++S+ +RG+LPII GGSNSY+E LVD   + F+SRY+CCFLWVDV++PVL  FVS+RVDK
Sbjct: 123 IESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSERVDK 182

Query: 181 MVAKGMVDEAEKFFDPAR-DYSRGIKRAIGVPEFDSYFRYKSFLDEETREKLLQESISKV 240
           MV  GMV+E  +FFD +  DYSRGIK+AIG PEFD +FR + FL+ E RE+LL + + ++
Sbjct: 183 MVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRFFRNEQFLNVEDREELLSKVLEEI 242

Query: 241 KEHTCKLALRQLGKIQRLINIKEWNIHRLDATEAFTKRGK--EAEEAWNKLVTARSTTII 299
           K +T +LA RQ  KI+RL  +K+W+I R+DAT  FTKR    +A  AW +LV   ST  +
Sbjct: 243 KRNTFELACRQREKIERLRKVKKWSIQRVDATPVFTKRRSKMDANVAWERLVAGPSTDTV 302

[Close]

BLAST of Cp4.1LG12g11680 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 308.5 bits (789), Expect = 6.4e-84
Identity = 161/275 (58.55%), Postives = 202/275 (73.45%), Query Frame = 0


Query: 29  PFFNLPKLGKEKVVIVIGATGTGKSRLSIDIATRYSMEVINSDKMQVYDGLDIVTNKVTK 88
           PFF      K+KVV V+GATGTGKSRL+ID+ATR+  E++NSDK+QVY GLDIVTNKVT 
Sbjct: 27  PFFR----RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDIVTNKVTP 86

Query: 89  REQRGVPHHLLGVI-DPFCDFSATDFSDMTMLSLDSICSRGRLPIIAGGSNSYIETLVDS 148
            E  GVPHHLLG + D + DF+A DF    + +++SI  R R+PIIAGGSNSYIE LV+ 
Sbjct: 87  EESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALVND 146

Query: 149 --NFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVAKGMVDEAEKFFDP-ARDYSRGIKRA 208
             +F+ RY CCFLWVDVS PVL SFVS+RVDKMV  G+VDE  + FDP + DYS GI+RA
Sbjct: 147 CVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDPSSSDYSAGIRRA 206

Query: 209 IGVPEFDSYFRYK-SFLDEETREKLLQESISKVKEHTCKLALRQLGKIQRLINIKEWNIH 268
           IGVPE D + R +      ET E+LL+ +I K+KE+TC LA RQL KIQRL    +WN+H
Sbjct: 207 IGVPELDEFLRSEMRNYPAETTERLLETAIEKIKENTCLLACRQLQKIQRLYKQWKWNMH 266

Query: 269 RLDATEAFTKRGKEAEEAWNKLVTARSTTIIDQFL 299
           R+DATE F +RG+EA+EAW+  V   S   +++FL
Sbjct: 267 RVDATEVFLRRGEEADEAWDNSVAHPSALAVEKFL 297

[Close]

BLAST of Cp4.1LG12g11680 vs. TAIR 10
Match: AT3G23630.1 (isopentenyltransferase 7 )

HSP 1 Score: 276.2 bits (705), Expect = 3.5e-74
Identity = 140/279 (50.18%), Postives = 197/279 (70.61%), Query Frame = 0


Query: 24  RINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIATRYSMEVINSDKMQVYDGLDIVT 83
           ++N N F +     KEKV+ V+GATG+GKSRL+ID+ATR+  E+INSDK+Q+Y GLD++T
Sbjct: 23  KVNVNSFLH----PKEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLT 82

Query: 84  NKVTKREQRGVPHHLLGVIDPFC-DFSATDFSDMTMLSLDSICSRGRLPIIAGGSNSYIE 143
           NKVT +E RGVPHHLLGV D    + +AT +S +   ++  + +  +LPI+AGGSNSYIE
Sbjct: 83  NKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIE 142

Query: 144 TLVDSN---FQSRYECCFLWVDVSIPVLQSFVSDRVDKMVAKGMVDEAEKFFDPARDYSR 203
            LV+ +     + Y+CCF+WVDVS+PVL SFVS RVD+M+  G+++E  + F+P  +YS 
Sbjct: 143 ALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSV 202

Query: 204 GIKRAIGVPEFDSYFRYKSFLDEETREKLLQESISKVKEHTCKLALRQLGKIQRLINIKE 263
           GI+RAIGVPE   Y R +S +D  T+ K+L  ++  +K++T  LA RQL KIQRL    +
Sbjct: 203 GIRRAIGVPELHEYLRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLHKKWK 262

Query: 264 WNIHRLDATEAFTKRG-KEAEEAWNKLVTARSTTIIDQF 298
            ++HR+DATE F KR  +E +EAW  LV   S  I+D+F
Sbjct: 263 MSMHRVDATEVFLKRNVEEQDEAWENLVARPSERIVDKF 297

[Close]

BLAST of Cp4.1LG12g11680 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 229.6 bits (584), Expect = 3.8e-60
Identity = 131/327 (40.06%), Postives = 196/327 (59.94%), Query Frame = 0


Query: 2   NVSTMFMSKEI--ITQPLINL-PPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSID 61
           N+++ F+S  +  IT P + L PPR +       + +  K+KVV+++GATG+GKS LSID
Sbjct: 3   NLTSTFVSPSMIPITSPRLRLPPPRSVVPMTTVCMEQSYKQKVVVIMGATGSGKSCLSID 62

Query: 62  IATRYSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDP-FCDFSATDFSDMT 121
           +ATR+S E++NSDK+Q YDGL + TN+++  E+ GVPHHLLG + P   + + ++F  + 
Sbjct: 63  LATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHHLLGELPPDDSELTTSEFRSLA 122

Query: 122 MLSLDSICSRGRLPIIAGGSNSYIETLVDSNFQS---------------RYECCFLWVDV 181
             S+  I +RG LPIIAGGSNS+I  L+   F                 RYECCFLWVDV
Sbjct: 123 SRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTYPFSSETSISSGLRYECCFLWVDV 182

Query: 182 SIPVLQSFVSDRVDKMVAKGMVDEAEKFFDPARDYS----RGIKRAIGVPEFDSYF---- 241
           S+ VL  ++S RVD+M+  GM +E   F+DP    S     GI + IG+PEFD YF    
Sbjct: 183 SVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIRAHGIHKTIGIPEFDRYFSLYP 242

Query: 242 ---RYKSFLDEETREKLLQESISKVKEHTCKLALRQLGKIQRLINIKEWNIHRLDATEAF 299
              + K    ++ R+    E++ ++KE+T +LA +Q+ +I +L     W+I RLDAT +F
Sbjct: 243 PERKQKMSEWDQARKGAYDEAVQEIKENTWRLAKKQIERIMKL-KSSGWDIQRLDATPSF 302

[Close]

BLAST of Cp4.1LG12g11680 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 217.6 bits (553), Expect = 1.5e-56
Identity = 127/333 (38.14%), Postives = 190/333 (57.06%), Query Frame = 0


Query: 4   STMFMSKEIITQPLINLPPRRINSNPFFNLPKL-------GKEKVVIVIGATGTGKSRLS 63
           S  F S    +  L++   RR    P  +  ++        K+KVV+++GATG GKSRLS
Sbjct: 23  SPSFSSHSSSSSSLLSFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSRLS 82

Query: 64  IDIATRYSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDP-FCDFSATDFSD 123
           +D+ATR+  E+INSDK+QVY+GL+I TN++T +++RGVPHHLLGVI+P   + +A +F  
Sbjct: 83  VDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEFRS 142

Query: 124 MTMLSLDSICSRGRLPIIAGGSNSYIETLVDSNFQS----------------RYECCFLW 183
                +  I SR ++PIIAGGSNS++  L+   F                  RYECCF+W
Sbjct: 143 AASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCFIW 202

Query: 184 VDVSIPVLQSFVSDRVDKMVAKGMVDEAEKFFDPAR---DYSRGIKRAIGVPEFDSYFR- 243
           VDVS  VL  ++  RVD+M+  GM +E  +F+DP +   +   GI++AIGVPEFD YF+ 
Sbjct: 203 VDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYFKE 262

Query: 244 ----YKSFLDEETREKLLQESISKVKEHTCKLALRQLGKIQRLINIKEWNIHRLDATEAF 299
                K    +  R+    +++  +K +T  LA RQ+ KI+ ++    W I R+DAT +F
Sbjct: 263 YPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKIE-MLKDAGWEIERVDATASF 322

The following BLAST results are available for this feature:

BLAST of Cp4.1LG12g11680 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93WC9	2.1e-87	53.29	Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q94ID2	9.0e-83	58.55	Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q94ID1	4.9e-73	50.18	Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=370...	[more]
Q9LJL4	5.3e-59	40.06	Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q5GHF7	9.0e-59	43.00	Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1	[more]

BLAST of Cp4.1LG12g11680 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023547390.1	1.02e-237	100.00	adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita pepo subsp. pe...	[more]
KAG7014428.1	2.57e-231	97.59	Adenylate isopentenyltransferase 3, chloroplastic, partial [Cucurbita argyrosper...	[more]
XP_022954005.1	2.36e-227	96.08	adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita moschata]	[more]
XP_022992534.1	9.96e-224	95.18	adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita maxima]	[more]
KAG6575896.1	3.57e-206	97.62	Adenylate isopentenyltransferase 3, chloroplastic, partial [Cucurbita argyrosper...	[more]

BLAST of Cp4.1LG12g11680 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GR83	1.14e-227	96.08	adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita moschata OX=...	[more]
A0A6J1JXT3	4.82e-224	95.18	adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita maxima OX=36...	[more]
A0A6J1KSJ2	1.02e-186	80.12	adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita maxima OX=36...	[more]
A0A6J1H8C9	4.82e-185	80.12	adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita moschata OX=...	[more]
A0A5D3DBM7	1.13e-183	80.42	Adenylate isopentenyltransferase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]

BLAST of Cp4.1LG12g11680 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G63110.1	1.5e-88	53.29	isopentenyltransferase 3	[more]
AT5G19040.1	6.4e-84	58.55	isopentenyltransferase 5	[more]
AT3G23630.1	3.5e-74	50.18	isopentenyltransferase 7	[more]
AT3G19160.1	3.8e-60	40.06	ATP/ADP isopentenyltransferases	[more]
AT1G68460.1	1.5e-56	38.14	isopentenyltransferase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF01715	IPPT	coord: 151..248 e-value: 2.1E-10 score: 40.7 coord: 74..147 e-value: 7.8E-21 score: 74.9
None	No IPR available	GENE3D	1.10.287.890	Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domain	coord: 164..262 e-value: 3.9E-105 score: 352.8
None	No IPR available	PANTHER	PTHR11088:SF74	ADENYLATE ISOPENTENYLTRANSFERASE 5, CHLOROPLASTIC	coord: 36..301
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 38..298 e-value: 3.9E-105 score: 352.8
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 37..191
IPR039657	Dimethylallyltransferase	PANTHER	PTHR11088	TRNA DIMETHYLALLYLTRANSFERASE	coord: 36..301

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG12g11680.1	Cp4.1LG12g11680.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009691	cytokinin biosynthetic process
biological_process	GO:0006400	tRNA modification
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005634	nucleus
cellular_component	GO:0009536	plastid
molecular_function	GO:0009824	AMP dimethylallyltransferase activity
molecular_function	GO:0052381	tRNA dimethylallyltransferase activity