Cp4.1LG12g09550 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG12g09550
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionO-fucosyltransferase family protein
LocationCp4.1LG12: 5539252 .. 5542471 (-)
RNA-Seq ExpressionCp4.1LG12g09550
SyntenyCp4.1LG12g09550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAGAAAAAGGGGAAAGGATATGAAAACGAAGGCTTAGGAGAAGCAATGAATTCGGGAGGGAAGTGTCGGTGCTGTGTGTTATAAAGAAGAAGAAACACAGAGAGAGAGGTTGAAGAACTGTCGTAGTGTTCTCTGTGATTCAGCGGCGGAAATTTCTTGAATCTTCGGCTTTGGCTTCCCCAAGTAATCAAGGAGCTTTCAATTCCATCCGCCATTGATTCCTCTGCTTAATCTTCACAGACTCTGGGAGAGAGAGAAGAGCAGGATCTCTGAACGGCAATGGGGGTGGATCTGAGACAAGTCTTGGCCGGCATACTCACCCTCACTATGTTCGTGATGCTTGGCCACATGATCAAGCGAGACCACTTTGATTCCGTCCAAGTACATGCTCTGAACCTTCATTTCCTTTTCGATCTGCTATTTCCTCCATTTTCTCTTCTAGAGATCTTATGCTGTGCAATCCGTGTCTTCTCTGTTTTCTTTAGTTTGCGTAGTCTGATTTATTTTCATGGTTTCTTACTTTTACTTTACTTTTTTATCATCATTCTTGCTCAATTTTGTGGAATTTAGTTCTGTATGAGTATAGCTTGACTAGTAGTAGCAGGAAGCGTACTATCTGTGCTTCACCGAGATAGAGTATAACTTACGGAATTTACAAGCTATGTTCGTTCAGCTTCTCTAATTTATCGGTAGAATCAAGCACTTTTTTCCTTTGAATATTTGATTGTGTGATTTTTTGCTTCTTACAAATCAAGTTTAGGAAATCGGAATCCTAATTCATTGCATTCAATCTTGCTTTTGTTTTGAAGGAAAAATTTCCAGGGCCAACGAAGGATGTTGCCAAAGTAACACCAGTGCGCAGCCTCAACAAAAAAGGTGCTATTCCGTGGAAAAATGATGTTCAAGAGCTAAAACAATGTTGGAGCAAACCCGAATCTGGTTAGTATTCGAAAATGAGCGAAAGTTAACTGATACGTTCATTTACCATTTATCGTGCTTTTGTATTTCTTATCCTTTCTATTCACTTATTACTCATTTGGATTGGAAGCAGACGAGGGGAGGCAACCAAAGGGGTATGTCACTTTTTCATTGACCGACGGTCCCGAATACCATGTCTCACAGGTACCCAAGTAAAGTATTTGATTCTTCTTGTTTTTGTCTAGCTTGTTCATCATTTTTCCATATAAACAATTAACTCGATAGAGCACTGCACATTATGACTGGGGTTCATGTTTTCTATTGGCTAATTAACCGTCATTTATATGAAGTAATTGTTGGAGTCCCACGTTGGCTAATTTAGGGAATGATCATGGGTTTATAAGTAAGGAATACATCTCCATTGGTATGAAGCCTTTTGGGGAAGTCCAAAGCAAATCGATTATGCCCAAAGTGGACAATATCATACCACTGTGGAGAGTTGTGATTCGTAACAGTAATGCGAGCAATACTCAGGGTCTCTTACTCTAAAAACTCCCCATTTGTGGCATTATTATTTGGGTTCTTCTCAGATCGTGTCACTTGTTGTCATCTAGCATTGTCTTCCCTTCATTTTACCACATTTCTTATTGATGCTTATTGCCTATTTCAGATTGCTGATGCTGTGGTCGTGGCTAGATATCTAGGTGCGACTCTTGTTGTCCCTGATATTAGAGGGAAAGAACTGGGAGATAAGTGGTAAGTTCTAACAAGTGCTGTGGCTGGAACAAGATCCTCCTTCTAAAGAATCTTCATCTCTACTTTTATTGCCTTGGAATTTCCTATTGCTTCACTCCATATTCATAGATATGTGCTGCTGTACTCTGTCTATTACTTCTGGGGACACTATTCTGGTCGAAACCTGACATAATTGGTATCTTCCAGGAGCTTTGGGGACATTTATGATGTTGAGAAGTTCATTGAAAGCCTCGATGGAGTTGTCAAAGTAGTAAAAACGCTGCCATCTGAAATATCTCCAAGAAAGCTCTCGACTGTCAAAGTGCCGAATCGGGTTACAGAAGATTACATTTCGGAACACGTAGAGAAGGTTTTTAAAAAAAGTGGCAGCATAAGACTCGCAACTTACTTTCCTTCCGTAAATATGAAAAAGTCGACCACATCAAGTGATGGTGATTCAGTTCCATGTTTGGCCATGTTTGGAACATTGGAGTTGCAGCCAGAAATAAGTGAGGTGATTGAATCCATGGTTGAGAGGCTAAAAACTTTGAGTCGGAAGTCTAACGGTCAGTTCATAGCTGTGGACTTGAGGATCGAAATGTTGGGGGAAAATGGATGCCAAGAGGCATCTGGAAGTAAAAGTTGTTACACTGCACAAGAAATAGCTTTGTTTTTGAGGAAGATTGGTTTCAACAAGGATGCTACCATATACTTGACTCAACCGAGATGGGAAAACAGTCTTGATGATCTCAAAGATTTGTTTCCAAAAACTTACACAAAGGTGAGTCTGGATGCATTTATCGTTCCCCGATACGTACTTCAATTTCGTGAGGATATATTTCGACAAATTAAGCTAGAAAGATTACTTCTAATGTTTTTTATTCCTCTGCTGCCTTTTCTTTAAGTTTGTTTGGATAGAATTCGCAACTCGGACCAAACTACGAATTCTTTCTCTTTATGAGAGTTAAGCTGTTCATCCATTACTGATTCTGGGCGTTTCTCCCAACTTTTGTTGCAGGAAAGCATAATGCCAGCGGACAAGAAGGCAAAGTTTTTGAATGCAGAGAGTTCTGAATACGAGAAAGTAATCGACTTCTACATATGTAGCCAAAGCGATATCTTCGTACCAGCGATCTCTGGACTCTTCTACTCGAATGTTGCAGGCAAGAGGATTGGTTTGGGCAAGAATCAAATACTTGTTCCAGCCACCATTAGAGAATCCACAGCTTCTGCTTCCAAATTCATCTCCTCTTACATCAGAAAGAAGAATCACTTGGCATATTCATGCTTTTGTTAGGCTCATTGTAAAGGTAAGTGAAGACACGACTGTATCGATCTCTGATTTGCGTGCTTCGAGCTTTCATGGCTGAGCAGATTACAAGGCCAATTGTATTGCATTGCATTAGTTTTCATTTTGTTCTTAATGATTCCTAAACAGAAGAGTATTTGTTAAAGATAATTTTGTAAGACATGCAGCAGAACGATTAAGATGTCAATTATTGTTTGATAAAGGCTTCCATTTACAGTAATGAGATTGAGAGAAAGTATTACATTTTGTGTTCAAGT

mRNA sequence

TAAGAAAAAGGGGAAAGGATATGAAAACGAAGGCTTAGGAGAAGCAATGAATTCGGGAGGGAAGTGTCGGTGCTGTGTGTTATAAAGAAGAAGAAACACAGAGAGAGAGGTTGAAGAACTGTCGTAGTGTTCTCTGTGATTCAGCGGCGGAAATTTCTTGAATCTTCGGCTTTGGCTTCCCCAAGTAATCAAGGAGCTTTCAATTCCATCCGCCATTGATTCCTCTGCTTAATCTTCACAGACTCTGGGAGAGAGAGAAGAGCAGGATCTCTGAACGGCAATGGGGGTGGATCTGAGACAAGTCTTGGCCGGCATACTCACCCTCACTATGTTCGTGATGCTTGGCCACATGATCAAGCGAGACCACTTTGATTCCGTCCAAGAAAAATTTCCAGGGCCAACGAAGGATGTTGCCAAAGTAACACCAGTGCGCAGCCTCAACAAAAAAGGTGCTATTCCGTGGAAAAATGATGTTCAAGAGCTAAAACAATGTTGGAGCAAACCCGAATCTGACGAGGGGAGGCAACCAAAGGGGTATGTCACTTTTTCATTGACCGACGGTCCCGAATACCATGTCTCACAGATTGCTGATGCTGTGGTCGTGGCTAGATATCTAGGTGCGACTCTTGTTGTCCCTGATATTAGAGGGAAAGAACTGGGAGATAAGTGGAGCTTTGGGGACATTTATGATGTTGAGAAGTTCATTGAAAGCCTCGATGGAGTTGTCAAAGTAGTAAAAACGCTGCCATCTGAAATATCTCCAAGAAAGCTCTCGACTGTCAAAGTGCCGAATCGGGTTACAGAAGATTACATTTCGGAACACGTAGAGAAGGTTTTTAAAAAAAGTGGCAGCATAAGACTCGCAACTTACTTTCCTTCCGTAAATATGAAAAAGTCGACCACATCAAGTGATGGTGATTCAGTTCCATGTTTGGCCATGTTTGGAACATTGGAGTTGCAGCCAGAAATAAGTGAGGTGATTGAATCCATGGTTGAGAGGCTAAAAACTTTGAGTCGGAAGTCTAACGGTCAGTTCATAGCTGTGGACTTGAGGATCGAAATGTTGGGGGAAAATGGATGCCAAGAGGCATCTGGAAGTAAAAGTTGTTACACTGCACAAGAAATAGCTTTGTTTTTGAGGAAGATTGGTTTCAACAAGGATGCTACCATATACTTGACTCAACCGAGATGGGAAAACAGTCTTGATGATCTCAAAGATTTGTTTCCAAAAACTTACACAAAGGAAAGCATAATGCCAGCGGACAAGAAGGCAAAGTTTTTGAATGCAGAGAGTTCTGAATACGAGAAAGTAATCGACTTCTACATATGTAGCCAAAGCGATATCTTCGTACCAGCGATCTCTGGACTCTTCTACTCGAATGTTGCAGGCAAGAGGATTGGTTTGGGCAAGAATCAAATACTTGTTCCAGCCACCATTAGAGAATCCACAGCTTCTGCTTCCAAATTCATCTCCTCTTACATCAGAAAGAAGAATCACTTGGCATATTCATGCTTTTGTTAGGCTCATTGTAAAGGTAAGTGAAGACACGACTGTATCGATCTCTGATTTGCGTGCTTCGAGCTTTCATGGCTGAGCAGATTACAAGGCCAATTGTATTGCATTGCATTAGTTTTCATTTTGTTCTTAATGATTCCTAAACAGAAGAGTATTTGTTAAAGATAATTTTGTAAGACATGCAGCAGAACGATTAAGATGTCAATTATTGTTTGATAAAGGCTTCCATTTACAGTAATGAGATTGAGAGAAAGTATTACATTTTGTGTTCAAGT

Coding sequence (CDS)

ATGGGGGTGGATCTGAGACAAGTCTTGGCCGGCATACTCACCCTCACTATGTTCGTGATGCTTGGCCACATGATCAAGCGAGACCACTTTGATTCCGTCCAAGAAAAATTTCCAGGGCCAACGAAGGATGTTGCCAAAGTAACACCAGTGCGCAGCCTCAACAAAAAAGGTGCTATTCCGTGGAAAAATGATGTTCAAGAGCTAAAACAATGTTGGAGCAAACCCGAATCTGACGAGGGGAGGCAACCAAAGGGGTATGTCACTTTTTCATTGACCGACGGTCCCGAATACCATGTCTCACAGATTGCTGATGCTGTGGTCGTGGCTAGATATCTAGGTGCGACTCTTGTTGTCCCTGATATTAGAGGGAAAGAACTGGGAGATAAGTGGAGCTTTGGGGACATTTATGATGTTGAGAAGTTCATTGAAAGCCTCGATGGAGTTGTCAAAGTAGTAAAAACGCTGCCATCTGAAATATCTCCAAGAAAGCTCTCGACTGTCAAAGTGCCGAATCGGGTTACAGAAGATTACATTTCGGAACACGTAGAGAAGGTTTTTAAAAAAAGTGGCAGCATAAGACTCGCAACTTACTTTCCTTCCGTAAATATGAAAAAGTCGACCACATCAAGTGATGGTGATTCAGTTCCATGTTTGGCCATGTTTGGAACATTGGAGTTGCAGCCAGAAATAAGTGAGGTGATTGAATCCATGGTTGAGAGGCTAAAAACTTTGAGTCGGAAGTCTAACGGTCAGTTCATAGCTGTGGACTTGAGGATCGAAATGTTGGGGGAAAATGGATGCCAAGAGGCATCTGGAAGTAAAAGTTGTTACACTGCACAAGAAATAGCTTTGTTTTTGAGGAAGATTGGTTTCAACAAGGATGCTACCATATACTTGACTCAACCGAGATGGGAAAACAGTCTTGATGATCTCAAAGATTTGTTTCCAAAAACTTACACAAAGGAAAGCATAATGCCAGCGGACAAGAAGGCAAAGTTTTTGAATGCAGAGAGTTCTGAATACGAGAAAGTAATCGACTTCTACATATGTAGCCAAAGCGATATCTTCGTACCAGCGATCTCTGGACTCTTCTACTCGAATGTTGCAGGCAAGAGGATTGGTTTGGGCAAGAATCAAATACTTGTTCCAGCCACCATTAGAGAATCCACAGCTTCTGCTTCCAAATTCATCTCCTCTTACATCAGAAAGAAGAATCACTTGGCATATTCATGCTTTTGTTAG

Protein sequence

MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIPWKNDVQELKQCWSKPESDEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPDIRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDYISEHVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEISEVIESMVERLKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYLTQPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYICSQSDIFVPAISGLFYSNVAGKRIGLGKNQILVPATIRESTASASKFISSYIRKKNHLAYSCFC
Homology
BLAST of Cp4.1LG12g09550 vs. ExPASy Swiss-Prot
Match: Q0WPA5 (Protein MANNAN SYNTHESIS-RELATED 2 OS=Arabidopsis thaliana OX=3702 GN=MSR2 PE=2 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 3.6e-150
Identity = 265/423 (62.65%), Postives = 330/423 (78.01%), Query Frame = 0

Query: 1   MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDV----AKVTP----VRS 60
           MGVDLRQV+AGILT+TMFVMLG M+ RD+FD+VQEK  G   D+    +KV      VR+
Sbjct: 1   MGVDLRQVVAGILTITMFVMLGQMLHRDYFDAVQEKVQGDAHDIEFHGSKVAVEDGLVRA 60

Query: 61  LNKKGAIPWKNDVQELKQCWSKPESDEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARYL 120
                  PW  D  ELK CWS  +SDE    KGYVTFSLT+GPEYHVSQI DAV+VA++L
Sbjct: 61  FEAGTKGPWMEDSHELKPCWSISQSDEAVSSKGYVTFSLTNGPEYHVSQITDAVMVAKHL 120

Query: 121 GATLVVPDIRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNR 180
           GATLV+PDIRG + GD+  F DIYDV+K I++L+ VVKVV+ LPS +S R ++ VKVP R
Sbjct: 121 GATLVLPDIRGSKPGDEMKFEDIYDVDKLIKTLESVVKVVRKLPSHVSLRDIAIVKVPTR 180

Query: 181 VTEDYISEHVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEISE 240
           V EDYI EH++ +FK  G+IR+ TYFPSVN++KS+  ++ D V CLAMFG+LELQP ++E
Sbjct: 181 VAEDYIKEHIDPIFKSKGNIRVTTYFPSVNLRKSSQGAETDPVSCLAMFGSLELQPAVNE 240

Query: 241 VIESMVERLKTLSRKSNGQFIAVDLRIEMLGENGCQE--ASGSKSCYTAQEIALFLRKIG 300
           ++ESM++RLKT S+KS G+FIA+DLR+E+L +  C E  A GSK+CY AQEIALFLRK+G
Sbjct: 241 LVESMIQRLKTHSKKSGGRFIAIDLRVEILEKKNCHETGAVGSKTCYNAQEIALFLRKLG 300

Query: 301 FNKDATIYLTQPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYIC 360
           F+ D TIYLTQPRWE+SL+ LKD+FPKTYTKE+IMP+DKK K+L  E+SEYE VIDFYI 
Sbjct: 301 FDSDTTIYLTQPRWESSLNILKDIFPKTYTKEAIMPSDKKTKYLELENSEYENVIDFYIS 360

Query: 361 SQSDIFVPAISGLFYSNVAGKRIGLGKNQILVPATIRESTASASKFISSYIRKKNHLAYS 414
           S+SD+FVPAI GLFY+N  GKRI LGK Q+LVPA I  ++   + +IS YI KKNHLAYS
Sbjct: 361 SRSDVFVPAIPGLFYANTVGKRIALGKPQVLVPAEISGTSGLPANYISPYISKKNHLAYS 420

BLAST of Cp4.1LG12g09550 vs. ExPASy Swiss-Prot
Match: Q9LU40 (Protein MANNAN SYNTHESIS-RELATED 1 OS=Arabidopsis thaliana OX=3702 GN=MSR1 PE=2 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 1.4e-146
Identity = 265/424 (62.50%), Postives = 328/424 (77.36%), Query Frame = 0

Query: 1   MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDV----AKVTPVRSL--- 60
           MGVDLRQV+AGILT+TMFVMLG M+ RD+FDS+QEK  G  +D+    +KV+    L   
Sbjct: 1   MGVDLRQVVAGILTITMFVMLGQMLHRDYFDSLQEKAQGDAQDIEFEGSKVSVKDGLVGT 60

Query: 61  --NKKGAIPWKNDVQELKQCWSKPESDEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARY 120
               KG   W  D  +L  CW    SD+    KGYVTFSLT+GPEYH+SQI DAV+VA++
Sbjct: 61  VEGSKGL--WMEDNTDLTPCWPTLLSDDAVSSKGYVTFSLTNGPEYHISQITDAVMVAKH 120

Query: 121 LGATLVVPDIRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPN 180
           LGATLV+PDIRG + GD+ +F DIYD +K I+SL+ VVKVVK LP E+S R ++ VKVP 
Sbjct: 121 LGATLVLPDIRGSKPGDERNFEDIYDADKLIKSLENVVKVVKKLPEEVSLRNMAIVKVPT 180

Query: 181 RVTEDYISEHVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEIS 240
           RVTEDYI EH++ +FK  G+IR+A+YFPSVN++KS+   + D V CLAMFG+LELQPE++
Sbjct: 181 RVTEDYIKEHIDPIFKSKGNIRVASYFPSVNLRKSSQDGETDPVACLAMFGSLELQPEVN 240

Query: 241 EVIESMVERLKTLSRKSNGQFIAVDLRIEMLGENGCQEAS--GSKSCYTAQEIALFLRKI 300
            V ESMVERL+T SRKS G+FIAVDLRI++L +  C      GSK+CY AQEIALFLRK+
Sbjct: 241 AVAESMVERLRTHSRKSGGRFIAVDLRIDILEKKNCHTTGVVGSKTCYNAQEIALFLRKL 300

Query: 301 GFNKDATIYLTQPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYI 360
           GF  D TIYLTQPRW++SL+ LKD+FPKT+TKE+IMPA K++K+L + SSEYE VIDFYI
Sbjct: 301 GFASDTTIYLTQPRWDSSLNILKDIFPKTFTKEAIMPASKRSKYLESVSSEYENVIDFYI 360

Query: 361 CSQSDIFVPAISGLFYSNVAGKRIGLGKNQILVPATIRESTASASKFISSYIRKKNHLAY 414
            S+SD+FVPAISGLFY+N  GKRI LGK Q+LVPA I E++  A+ FIS YI KKNHLAY
Sbjct: 361 SSRSDVFVPAISGLFYANTVGKRIALGKPQVLVPAEISETSGLATDFISPYISKKNHLAY 420

BLAST of Cp4.1LG12g09550 vs. ExPASy Swiss-Prot
Match: K7R4D4 (Protein MANNAN SYNTHESIS-RELATED OS=Trigonella foenum-graecum OX=78534 GN=MSR PE=2 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 1.9e-114
Identity = 210/416 (50.48%), Postives = 298/416 (71.63%), Query Frame = 0

Query: 3   VDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAK--VTPVRSLNKKGAIP 62
           +++RQ  AG+LTL+MF+MLG+MIK+DHFD   E+    T +V++  +  V  +++K    
Sbjct: 4   MEIRQAFAGLLTLSMFIMLGNMIKKDHFDYPAEEVEIQTTEVSQHDLATVSHISQKS--- 63

Query: 63  WKNDVQELKQCWSKPESDEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPD 122
            K + + LK CW+ P   E  Q KG++ FSLT+GPEYH++Q+ADAVVVA+YLGATLV+PD
Sbjct: 64  -KQNDKALKPCWNPPTLKEVEQSKGFIIFSLTNGPEYHIAQVADAVVVAKYLGATLVLPD 123

Query: 123 IRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDYISE 182
           I+  + G+  + GDIYDVE  +  L+G+VKV KTLP  +S R    V+VPN+V++DYI +
Sbjct: 124 IKNSKSGNSMNLGDIYDVENVLNKLNGLVKVTKTLPPHVSTRNTPIVRVPNKVSQDYIMK 183

Query: 183 HVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMF-GTLELQPEISEVIESMVE 242
            ++ +++  G I++ +YFPS N   S  ++  +S+ C  MF GTLEL+ EI E  ES+V+
Sbjct: 184 KLKPIYQAKGIIKIESYFPSKN-TISRNNNSLESLLCQTMFGGTLELKKEIQEEAESIVQ 243

Query: 243 RLKTLSRKSNGQFIAVDLRIEMLGENGC--QEASGSKSCYTAQEIALFLRKIGFNKDATI 302
           +L+T S++SNG F+AVDLRIE L +N C  ++  G K CY   EI  FL++IGF ++  I
Sbjct: 244 KLETWSQESNGPFVAVDLRIEGL-KNECNGKDGKGRKQCYQGHEIGEFLKRIGFGQETVI 303

Query: 303 YLTQPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYICSQSDIFV 362
           Y+TQ +W   L+ L+ +FPKTYTKE+IM + KK KF+N+ES E+EK IDFYICS+SD+FV
Sbjct: 304 YVTQTKWSPDLNSLRYMFPKTYTKENIMSSTKKEKFINSESIEFEKAIDFYICSESDVFV 363

Query: 363 PAISGLFYSNVAGKRIGLGKNQILVPATIRESTASASKFISSYIRKKNHLAYSCFC 414
           P+I G FY NVAG RI  GKN+I+VP+ +   +ASAS+ +S Y+ KKNHLAY CFC
Sbjct: 364 PSILGPFYENVAGMRIVSGKNEIIVPSEVVSPSASASEHMSPYVTKKNHLAYKCFC 413

BLAST of Cp4.1LG12g09550 vs. ExPASy Swiss-Prot
Match: Q8LNP3 (O-fucosyltransferase 10 OS=Arabidopsis thaliana OX=3702 GN=OFUT10 PE=2 SV=2)

HSP 1 Score: 116.7 bits (291), Expect = 6.2e-25
Identity = 98/343 (28.57%), Postives = 148/343 (43.15%), Query Frame = 0

Query: 76  ESDEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPDIRGKEL-GDKWSFGD 135
           ES       GY+      G     S I +AV VA  L A LV+P      +  D  +FGD
Sbjct: 19  ESSALAPSSGYLIVEANGGLNQQRSAICNAVAVAGLLNAVLVIPRFEFHAIWKDSSNFGD 78

Query: 136 IYDVEKFIESLDGVVKVVKTLPSEISPR------KLSTVKVPNRVTEDYISEHVEKVFKK 195
           IYD + FI SL+G VK+V+ +P EI  R       + T++V    T +Y +  V  V K+
Sbjct: 79  IYDEDHFISSLEGYVKIVRDVPDEIMTRFSYNVSSIPTIRVQAWATVNYYNGEVYPVLKE 138

Query: 196 SGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEISEVIESMVERLKTLSRKS 255
            G IR+    P  N    +       + C+A +  L+    IS + E +V+R+   S  +
Sbjct: 139 HGVIRIT---PFANRLAMSVPPYIQLLRCIANYKALKFSSPISTLAEKLVDRMVEKSSAT 198

Query: 256 NGQFIAVDLRIE--------MLGENGCQEAS----------------------------G 315
            G++++V LR E         L E G  E S                             
Sbjct: 199 GGKYVSVHLRFEEDMVAFSCCLYEGGRAEKSEMDVIRQKSWKGKFKRRDRVIRPDLNRVN 258

Query: 316 SKSCYTAQEIALFLRKIGFNKDATIYLTQPRW---ENSLDDLKDLFPKTYTKESIMPADK 373
            K   T  E+ + LR +GF+ + +IYL   R    E  L  L+++FP+ YTKES+   ++
Sbjct: 259 GKCPLTPLEVGMMLRGMGFDNNTSIYLASGRIYQPEKHLAPLQEMFPRLYTKESLATPEE 318

BLAST of Cp4.1LG12g09550 vs. ExPASy Swiss-Prot
Match: Q8GUM0 (O-fucosyltransferase 11 OS=Arabidopsis thaliana OX=3702 GN=OFUT11 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 6.2e-25
Identity = 98/343 (28.57%), Postives = 148/343 (43.15%), Query Frame = 0

Query: 76  ESDEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPDIRGKEL-GDKWSFGD 135
           ES       GY+      G     S I +AV VA  L A LV+P      +  D  +FGD
Sbjct: 148 ESSALAPSSGYLIVEANGGLNQQRSAICNAVAVAGLLNAVLVIPRFEFHAIWKDSSNFGD 207

Query: 136 IYDVEKFIESLDGVVKVVKTLPSEISPR------KLSTVKVPNRVTEDYISEHVEKVFKK 195
           IYD + FI SL+G VK+V+ +P EI  R       + T++V    T +Y +  V  V K+
Sbjct: 208 IYDEDHFISSLEGYVKIVRDVPDEIMTRFSYNVSSIPTIRVQAWATVNYYNGEVYPVLKE 267

Query: 196 SGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEISEVIESMVERLKTLSRKS 255
            G IR+    P  N    +       + C+A +  L+    IS + E +V+R+   S  +
Sbjct: 268 HGVIRIT---PFANRLAMSVPPYIQLLRCIANYKALKFSSPISTLAEKLVDRMVEKSSAT 327

Query: 256 NGQFIAVDLRIE--------MLGENGCQEAS----------------------------G 315
            G++++V LR E         L E G  E S                             
Sbjct: 328 GGKYVSVHLRFEEDMVAFSCCLYEGGRAEKSEMDVIRQKSWKGKFKRRDRVIRPDLNRVN 387

Query: 316 SKSCYTAQEIALFLRKIGFNKDATIYLTQPRW---ENSLDDLKDLFPKTYTKESIMPADK 373
            K   T  E+ + LR +GF+ + +IYL   R    E  L  L+++FP+ YTKES+   ++
Sbjct: 388 GKCPLTPLEVGMMLRGMGFDNNTSIYLASGRIYQPEKHLAPLQEMFPRLYTKESLATPEE 447

BLAST of Cp4.1LG12g09550 vs. NCBI nr
Match: XP_023548673.1 (protein MANNAN SYNTHESIS-RELATED 2 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 818 bits (2112), Expect = 1.09e-298
Identity = 413/413 (100.00%), Postives = 413/413 (100.00%), Query Frame = 0

Query: 1   MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP 60
           MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP
Sbjct: 1   MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP 60

Query: 61  WKNDVQELKQCWSKPESDEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPD 120
           WKNDVQELKQCWSKPESDEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPD
Sbjct: 61  WKNDVQELKQCWSKPESDEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPD 120

Query: 121 IRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDYISE 180
           IRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDYISE
Sbjct: 121 IRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDYISE 180

Query: 181 HVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEISEVIESMVER 240
           HVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEISEVIESMVER
Sbjct: 181 HVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEISEVIESMVER 240

Query: 241 LKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYLT 300
           LKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYLT
Sbjct: 241 LKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYLT 300

Query: 301 QPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYICSQSDIFVPAI 360
           QPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYICSQSDIFVPAI
Sbjct: 301 QPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYICSQSDIFVPAI 360

Query: 361 SGLFYSNVAGKRIGLGKNQILVPATIRESTASASKFISSYIRKKNHLAYSCFC 413
           SGLFYSNVAGKRIGLGKNQILVPATIRESTASASKFISSYIRKKNHLAYSCFC
Sbjct: 361 SGLFYSNVAGKRIGLGKNQILVPATIRESTASASKFISSYIRKKNHLAYSCFC 413

BLAST of Cp4.1LG12g09550 vs. NCBI nr
Match: XP_023548672.1 (protein MANNAN SYNTHESIS-RELATED 2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 813 bits (2100), Expect = 7.67e-297
Identity = 413/414 (99.76%), Postives = 413/414 (99.76%), Query Frame = 0

Query: 1   MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP 60
           MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP
Sbjct: 1   MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP 60

Query: 61  WKNDVQELKQCWSKPES-DEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVP 120
           WKNDVQELKQCWSKPES DEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVP
Sbjct: 61  WKNDVQELKQCWSKPESADEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVP 120

Query: 121 DIRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDYIS 180
           DIRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDYIS
Sbjct: 121 DIRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDYIS 180

Query: 181 EHVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEISEVIESMVE 240
           EHVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEISEVIESMVE
Sbjct: 181 EHVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEISEVIESMVE 240

Query: 241 RLKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYL 300
           RLKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYL
Sbjct: 241 RLKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYL 300

Query: 301 TQPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYICSQSDIFVPA 360
           TQPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYICSQSDIFVPA
Sbjct: 301 TQPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYICSQSDIFVPA 360

Query: 361 ISGLFYSNVAGKRIGLGKNQILVPATIRESTASASKFISSYIRKKNHLAYSCFC 413
           ISGLFYSNVAGKRIGLGKNQILVPATIRESTASASKFISSYIRKKNHLAYSCFC
Sbjct: 361 ISGLFYSNVAGKRIGLGKNQILVPATIRESTASASKFISSYIRKKNHLAYSCFC 414

BLAST of Cp4.1LG12g09550 vs. NCBI nr
Match: KAG6575401.1 (Protein MANNAN SYNTHESIS-RELATED 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 808 bits (2087), Expect = 7.08e-295
Identity = 407/413 (98.55%), Postives = 411/413 (99.52%), Query Frame = 0

Query: 1   MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP 60
           MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP
Sbjct: 1   MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP 60

Query: 61  WKNDVQELKQCWSKPESDEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPD 120
           WKNDVQELKQCWSKPESDEGRQ KGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPD
Sbjct: 61  WKNDVQELKQCWSKPESDEGRQSKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPD 120

Query: 121 IRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDYISE 180
           IRGKELGDKWSFGDIYDV+KFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDYISE
Sbjct: 121 IRGKELGDKWSFGDIYDVKKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDYISE 180

Query: 181 HVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEISEVIESMVER 240
           HVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEI+EVIESMVER
Sbjct: 181 HVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEINEVIESMVER 240

Query: 241 LKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYLT 300
           LKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYLT
Sbjct: 241 LKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYLT 300

Query: 301 QPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYICSQSDIFVPAI 360
           QPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFY+CSQSDIFVPAI
Sbjct: 301 QPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYVCSQSDIFVPAI 360

Query: 361 SGLFYSNVAGKRIGLGKNQILVPATIRESTASASKFISSYIRKKNHLAYSCFC 413
           SGLFYSNVAGKRIGLGKNQILVPATIRESTASAS FISSYI+KKNHLAYSCFC
Sbjct: 361 SGLFYSNVAGKRIGLGKNQILVPATIRESTASASNFISSYIQKKNHLAYSCFC 413

BLAST of Cp4.1LG12g09550 vs. NCBI nr
Match: KAG7013939.1 (hypothetical protein SDJN02_24108 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 808 bits (2086), Expect = 1.01e-294
Identity = 406/413 (98.31%), Postives = 411/413 (99.52%), Query Frame = 0

Query: 1   MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP 60
           MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP
Sbjct: 1   MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP 60

Query: 61  WKNDVQELKQCWSKPESDEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPD 120
           WKNDVQELKQCWSKPESDEGRQ KGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPD
Sbjct: 61  WKNDVQELKQCWSKPESDEGRQSKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPD 120

Query: 121 IRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDYISE 180
           IRGKELGDKWSFGDIYDV+KFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDYISE
Sbjct: 121 IRGKELGDKWSFGDIYDVKKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDYISE 180

Query: 181 HVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEISEVIESMVER 240
           H+EKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEI+EVIESMVER
Sbjct: 181 HIEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEINEVIESMVER 240

Query: 241 LKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYLT 300
           LKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYLT
Sbjct: 241 LKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYLT 300

Query: 301 QPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYICSQSDIFVPAI 360
           QPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFY+CSQSDIFVPAI
Sbjct: 301 QPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYVCSQSDIFVPAI 360

Query: 361 SGLFYSNVAGKRIGLGKNQILVPATIRESTASASKFISSYIRKKNHLAYSCFC 413
           SGLFYSNVAGKRIGLGKNQILVPATIRESTASAS FISSYI+KKNHLAYSCFC
Sbjct: 361 SGLFYSNVAGKRIGLGKNQILVPATIRESTASASNFISSYIQKKNHLAYSCFC 413

BLAST of Cp4.1LG12g09550 vs. NCBI nr
Match: XP_022992265.1 (protein MANNAN SYNTHESIS-RELATED 2 isoform X2 [Cucurbita maxima])

HSP 1 Score: 803 bits (2075), Expect = 4.77e-293
Identity = 405/413 (98.06%), Postives = 408/413 (98.79%), Query Frame = 0

Query: 1   MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP 60
           MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP
Sbjct: 1   MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP 60

Query: 61  WKNDVQELKQCWSKPESDEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPD 120
           WKNDVQELKQCWSKPE DEGRQ KGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPD
Sbjct: 61  WKNDVQELKQCWSKPEYDEGRQSKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPD 120

Query: 121 IRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDYISE 180
           IRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDY SE
Sbjct: 121 IRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDYTSE 180

Query: 181 HVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEISEVIESMVER 240
           HVEKVFKKSG+IRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEI+EVIESMVER
Sbjct: 181 HVEKVFKKSGNIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEINEVIESMVER 240

Query: 241 LKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYLT 300
           LKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYLT
Sbjct: 241 LKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYLT 300

Query: 301 QPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYICSQSDIFVPAI 360
           QPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFY+CSQSDIFVPAI
Sbjct: 301 QPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYVCSQSDIFVPAI 360

Query: 361 SGLFYSNVAGKRIGLGKNQILVPATIRESTASASKFISSYIRKKNHLAYSCFC 413
           SGLFYSNVAGKRIGLGKNQILVPATIRESTASAS FISSYI KKNHLAYSCFC
Sbjct: 361 SGLFYSNVAGKRIGLGKNQILVPATIRESTASASNFISSYITKKNHLAYSCFC 413

BLAST of Cp4.1LG12g09550 vs. ExPASy TrEMBL
Match: A0A6J1JV82 (O-fucosyltransferase family protein OS=Cucurbita maxima OX=3661 GN=LOC111488638 PE=3 SV=1)

HSP 1 Score: 803 bits (2075), Expect = 2.31e-293
Identity = 405/413 (98.06%), Postives = 408/413 (98.79%), Query Frame = 0

Query: 1   MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP 60
           MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP
Sbjct: 1   MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP 60

Query: 61  WKNDVQELKQCWSKPESDEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPD 120
           WKNDVQELKQCWSKPE DEGRQ KGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPD
Sbjct: 61  WKNDVQELKQCWSKPEYDEGRQSKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPD 120

Query: 121 IRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDYISE 180
           IRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDY SE
Sbjct: 121 IRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDYTSE 180

Query: 181 HVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEISEVIESMVER 240
           HVEKVFKKSG+IRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEI+EVIESMVER
Sbjct: 181 HVEKVFKKSGNIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEINEVIESMVER 240

Query: 241 LKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYLT 300
           LKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYLT
Sbjct: 241 LKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYLT 300

Query: 301 QPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYICSQSDIFVPAI 360
           QPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFY+CSQSDIFVPAI
Sbjct: 301 QPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYVCSQSDIFVPAI 360

Query: 361 SGLFYSNVAGKRIGLGKNQILVPATIRESTASASKFISSYIRKKNHLAYSCFC 413
           SGLFYSNVAGKRIGLGKNQILVPATIRESTASAS FISSYI KKNHLAYSCFC
Sbjct: 361 SGLFYSNVAGKRIGLGKNQILVPATIRESTASASNFISSYITKKNHLAYSCFC 413

BLAST of Cp4.1LG12g09550 vs. ExPASy TrEMBL
Match: A0A6J1GQV1 (O-fucosyltransferase family protein OS=Cucurbita moschata OX=3662 GN=LOC111456273 PE=3 SV=1)

HSP 1 Score: 803 bits (2073), Expect = 4.66e-293
Identity = 404/413 (97.82%), Postives = 408/413 (98.79%), Query Frame = 0

Query: 1   MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP 60
           MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP
Sbjct: 1   MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP 60

Query: 61  WKNDVQELKQCWSKPESDEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPD 120
           WKNDVQELKQCWSKPESDEGRQ KGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPD
Sbjct: 61  WKNDVQELKQCWSKPESDEGRQSKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPD 120

Query: 121 IRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDYISE 180
           IRGKELGDKWSFGDIYDV+KFIESLDGVVKVVKTLPSEISPRKLS +KVPNRVTEDYI E
Sbjct: 121 IRGKELGDKWSFGDIYDVKKFIESLDGVVKVVKTLPSEISPRKLSVIKVPNRVTEDYILE 180

Query: 181 HVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEISEVIESMVER 240
           HVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEI+EVIESMVER
Sbjct: 181 HVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEINEVIESMVER 240

Query: 241 LKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYLT 300
           LKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYLT
Sbjct: 241 LKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYLT 300

Query: 301 QPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYICSQSDIFVPAI 360
           QPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFY+CSQSDIFVPAI
Sbjct: 301 QPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYVCSQSDIFVPAI 360

Query: 361 SGLFYSNVAGKRIGLGKNQILVPATIRESTASASKFISSYIRKKNHLAYSCFC 413
           SGLFYSNVAGKRIGLGKNQILVPATIRESTASAS FISSYI KKNHLAYSCFC
Sbjct: 361 SGLFYSNVAGKRIGLGKNQILVPATIRESTASASNFISSYITKKNHLAYSCFC 413

BLAST of Cp4.1LG12g09550 vs. ExPASy TrEMBL
Match: A0A6J1JX30 (O-fucosyltransferase family protein OS=Cucurbita maxima OX=3661 GN=LOC111488638 PE=3 SV=1)

HSP 1 Score: 800 bits (2066), Expect = 5.65e-292
Identity = 405/414 (97.83%), Postives = 409/414 (98.79%), Query Frame = 0

Query: 1   MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP 60
           MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP
Sbjct: 1   MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP 60

Query: 61  WKNDVQELKQCWSKPE-SDEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVP 120
           WKNDVQELKQCWSKPE +DEGRQ KGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVP
Sbjct: 61  WKNDVQELKQCWSKPEYADEGRQSKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVP 120

Query: 121 DIRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDYIS 180
           DIRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDY S
Sbjct: 121 DIRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDYTS 180

Query: 181 EHVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEISEVIESMVE 240
           EHVEKVFKKSG+IRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEI+EVIESMVE
Sbjct: 181 EHVEKVFKKSGNIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEINEVIESMVE 240

Query: 241 RLKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYL 300
           RLKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYL
Sbjct: 241 RLKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYL 300

Query: 301 TQPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYICSQSDIFVPA 360
           TQPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFY+CSQSDIFVPA
Sbjct: 301 TQPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYVCSQSDIFVPA 360

Query: 361 ISGLFYSNVAGKRIGLGKNQILVPATIRESTASASKFISSYIRKKNHLAYSCFC 413
           ISGLFYSNVAGKRIGLGKNQILVPATIRESTASAS FISSYI KKNHLAYSCFC
Sbjct: 361 ISGLFYSNVAGKRIGLGKNQILVPATIRESTASASNFISSYITKKNHLAYSCFC 414

BLAST of Cp4.1LG12g09550 vs. ExPASy TrEMBL
Match: A0A6J1GPH2 (O-fucosyltransferase family protein OS=Cucurbita moschata OX=3662 GN=LOC111456273 PE=3 SV=1)

HSP 1 Score: 798 bits (2061), Expect = 3.27e-291
Identity = 404/414 (97.58%), Postives = 408/414 (98.55%), Query Frame = 0

Query: 1   MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP 60
           MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP
Sbjct: 1   MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP 60

Query: 61  WKNDVQELKQCWSKPES-DEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVP 120
           WKNDVQELKQCWSKPES DEGRQ KGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVP
Sbjct: 61  WKNDVQELKQCWSKPESADEGRQSKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVP 120

Query: 121 DIRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDYIS 180
           DIRGKELGDKWSFGDIYDV+KFIESLDGVVKVVKTLPSEISPRKLS +KVPNRVTEDYI 
Sbjct: 121 DIRGKELGDKWSFGDIYDVKKFIESLDGVVKVVKTLPSEISPRKLSVIKVPNRVTEDYIL 180

Query: 181 EHVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEISEVIESMVE 240
           EHVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEI+EVIESMVE
Sbjct: 181 EHVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEINEVIESMVE 240

Query: 241 RLKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYL 300
           RLKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYL
Sbjct: 241 RLKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYL 300

Query: 301 TQPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYICSQSDIFVPA 360
           TQPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFY+CSQSDIFVPA
Sbjct: 301 TQPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYVCSQSDIFVPA 360

Query: 361 ISGLFYSNVAGKRIGLGKNQILVPATIRESTASASKFISSYIRKKNHLAYSCFC 413
           ISGLFYSNVAGKRIGLGKNQILVPATIRESTASAS FISSYI KKNHLAYSCFC
Sbjct: 361 ISGLFYSNVAGKRIGLGKNQILVPATIRESTASASNFISSYITKKNHLAYSCFC 414

BLAST of Cp4.1LG12g09550 vs. ExPASy TrEMBL
Match: A0A6J1KJH2 (O-fucosyltransferase family protein OS=Cucurbita maxima OX=3661 GN=LOC111494558 PE=3 SV=1)

HSP 1 Score: 752 bits (1941), Expect = 6.09e-273
Identity = 375/413 (90.80%), Postives = 399/413 (96.61%), Query Frame = 0

Query: 1   MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDVAKVTPVRSLNKKGAIP 60
           MGVDLRQVLAGILTLTMF+MLGHMIKRDHFDSVQEKFPGPTK+V KVT +RSL+KKG   
Sbjct: 1   MGVDLRQVLAGILTLTMFLMLGHMIKRDHFDSVQEKFPGPTKEVTKVTTIRSLHKKGDAA 60

Query: 61  WKNDVQELKQCWSKPESDEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPD 120
            KNDVQELKQCWSKPE DEG+Q KGYVTFSLT+GPEYHVSQIADAVVVARYLGATLVVPD
Sbjct: 61  SKNDVQELKQCWSKPELDEGQQSKGYVTFSLTEGPEYHVSQIADAVVVARYLGATLVVPD 120

Query: 121 IRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNRVTEDYISE 180
           IRGK+LGDKW+FGDIYDVEKFI SLDGVVKVVK LPSEISPRK+S VKVPNRV+E+YISE
Sbjct: 121 IRGKKLGDKWNFGDIYDVEKFIGSLDGVVKVVKELPSEISPRKISAVKVPNRVSENYISE 180

Query: 181 HVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEISEVIESMVER 240
           HVEK++KKSGSIRLAT+FPSVNMKKSTTSS+GDSVPCLAMFGTL+LQPEISEVIESM+ER
Sbjct: 181 HVEKIYKKSGSIRLATFFPSVNMKKSTTSSNGDSVPCLAMFGTLKLQPEISEVIESMIER 240

Query: 241 LKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKSCYTAQEIALFLRKIGFNKDATIYLT 300
           LKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSK CYTAQ+IALFLRKIGF+KDATIYLT
Sbjct: 241 LKTLSRKSNGQFIAVDLRIEMLGENGCQEASGSKGCYTAQDIALFLRKIGFDKDATIYLT 300

Query: 301 QPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYICSQSDIFVPAI 360
           QPRW+NSL+DLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFY+CSQSDIFVPAI
Sbjct: 301 QPRWDNSLEDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYVCSQSDIFVPAI 360

Query: 361 SGLFYSNVAGKRIGLGKNQILVPATIRESTASASKFISSYIRKKNHLAYSCFC 413
           SGLFYSNVAGKRI LGKNQILVPATIRESTA A+KFISSYI+KKNHLAYSCFC
Sbjct: 361 SGLFYSNVAGKRIALGKNQILVPATIRESTAPATKFISSYIKKKNHLAYSCFC 413

BLAST of Cp4.1LG12g09550 vs. TAIR 10
Match: AT1G51630.1 (O-fucosyltransferase family protein )

HSP 1 Score: 532.7 bits (1371), Expect = 2.6e-151
Identity = 265/423 (62.65%), Postives = 330/423 (78.01%), Query Frame = 0

Query: 1   MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDV----AKVTP----VRS 60
           MGVDLRQV+AGILT+TMFVMLG M+ RD+FD+VQEK  G   D+    +KV      VR+
Sbjct: 1   MGVDLRQVVAGILTITMFVMLGQMLHRDYFDAVQEKVQGDAHDIEFHGSKVAVEDGLVRA 60

Query: 61  LNKKGAIPWKNDVQELKQCWSKPESDEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARYL 120
                  PW  D  ELK CWS  +SDE    KGYVTFSLT+GPEYHVSQI DAV+VA++L
Sbjct: 61  FEAGTKGPWMEDSHELKPCWSISQSDEAVSSKGYVTFSLTNGPEYHVSQITDAVMVAKHL 120

Query: 121 GATLVVPDIRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPNR 180
           GATLV+PDIRG + GD+  F DIYDV+K I++L+ VVKVV+ LPS +S R ++ VKVP R
Sbjct: 121 GATLVLPDIRGSKPGDEMKFEDIYDVDKLIKTLESVVKVVRKLPSHVSLRDIAIVKVPTR 180

Query: 181 VTEDYISEHVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEISE 240
           V EDYI EH++ +FK  G+IR+ TYFPSVN++KS+  ++ D V CLAMFG+LELQP ++E
Sbjct: 181 VAEDYIKEHIDPIFKSKGNIRVTTYFPSVNLRKSSQGAETDPVSCLAMFGSLELQPAVNE 240

Query: 241 VIESMVERLKTLSRKSNGQFIAVDLRIEMLGENGCQE--ASGSKSCYTAQEIALFLRKIG 300
           ++ESM++RLKT S+KS G+FIA+DLR+E+L +  C E  A GSK+CY AQEIALFLRK+G
Sbjct: 241 LVESMIQRLKTHSKKSGGRFIAIDLRVEILEKKNCHETGAVGSKTCYNAQEIALFLRKLG 300

Query: 301 FNKDATIYLTQPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYIC 360
           F+ D TIYLTQPRWE+SL+ LKD+FPKTYTKE+IMP+DKK K+L  E+SEYE VIDFYI 
Sbjct: 301 FDSDTTIYLTQPRWESSLNILKDIFPKTYTKEAIMPSDKKTKYLELENSEYENVIDFYIS 360

Query: 361 SQSDIFVPAISGLFYSNVAGKRIGLGKNQILVPATIRESTASASKFISSYIRKKNHLAYS 414
           S+SD+FVPAI GLFY+N  GKRI LGK Q+LVPA I  ++   + +IS YI KKNHLAYS
Sbjct: 361 SRSDVFVPAIPGLFYANTVGKRIALGKPQVLVPAEISGTSGLPANYISPYISKKNHLAYS 420

BLAST of Cp4.1LG12g09550 vs. TAIR 10
Match: AT3G21190.1 (O-fucosyltransferase family protein )

HSP 1 Score: 520.8 bits (1340), Expect = 1.0e-147
Identity = 265/424 (62.50%), Postives = 328/424 (77.36%), Query Frame = 0

Query: 1   MGVDLRQVLAGILTLTMFVMLGHMIKRDHFDSVQEKFPGPTKDV----AKVTPVRSL--- 60
           MGVDLRQV+AGILT+TMFVMLG M+ RD+FDS+QEK  G  +D+    +KV+    L   
Sbjct: 1   MGVDLRQVVAGILTITMFVMLGQMLHRDYFDSLQEKAQGDAQDIEFEGSKVSVKDGLVGT 60

Query: 61  --NKKGAIPWKNDVQELKQCWSKPESDEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARY 120
               KG   W  D  +L  CW    SD+    KGYVTFSLT+GPEYH+SQI DAV+VA++
Sbjct: 61  VEGSKGL--WMEDNTDLTPCWPTLLSDDAVSSKGYVTFSLTNGPEYHISQITDAVMVAKH 120

Query: 121 LGATLVVPDIRGKELGDKWSFGDIYDVEKFIESLDGVVKVVKTLPSEISPRKLSTVKVPN 180
           LGATLV+PDIRG + GD+ +F DIYD +K I+SL+ VVKVVK LP E+S R ++ VKVP 
Sbjct: 121 LGATLVLPDIRGSKPGDERNFEDIYDADKLIKSLENVVKVVKKLPEEVSLRNMAIVKVPT 180

Query: 181 RVTEDYISEHVEKVFKKSGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEIS 240
           RVTEDYI EH++ +FK  G+IR+A+YFPSVN++KS+   + D V CLAMFG+LELQPE++
Sbjct: 181 RVTEDYIKEHIDPIFKSKGNIRVASYFPSVNLRKSSQDGETDPVACLAMFGSLELQPEVN 240

Query: 241 EVIESMVERLKTLSRKSNGQFIAVDLRIEMLGENGCQEAS--GSKSCYTAQEIALFLRKI 300
            V ESMVERL+T SRKS G+FIAVDLRI++L +  C      GSK+CY AQEIALFLRK+
Sbjct: 241 AVAESMVERLRTHSRKSGGRFIAVDLRIDILEKKNCHTTGVVGSKTCYNAQEIALFLRKL 300

Query: 301 GFNKDATIYLTQPRWENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESSEYEKVIDFYI 360
           GF  D TIYLTQPRW++SL+ LKD+FPKT+TKE+IMPA K++K+L + SSEYE VIDFYI
Sbjct: 301 GFASDTTIYLTQPRWDSSLNILKDIFPKTFTKEAIMPASKRSKYLESVSSEYENVIDFYI 360

Query: 361 CSQSDIFVPAISGLFYSNVAGKRIGLGKNQILVPATIRESTASASKFISSYIRKKNHLAY 414
            S+SD+FVPAISGLFY+N  GKRI LGK Q+LVPA I E++  A+ FIS YI KKNHLAY
Sbjct: 361 SSRSDVFVPAISGLFYANTVGKRIALGKPQVLVPAEISETSGLATDFISPYISKKNHLAY 420

BLAST of Cp4.1LG12g09550 vs. TAIR 10
Match: AT1G38131.1 (O-fucosyltransferase family protein )

HSP 1 Score: 116.7 bits (291), Expect = 4.4e-26
Identity = 98/343 (28.57%), Postives = 148/343 (43.15%), Query Frame = 0

Query: 76  ESDEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPDIRGKEL-GDKWSFGD 135
           ES       GY+      G     S I +AV VA  L A LV+P      +  D  +FGD
Sbjct: 148 ESSALAPSSGYLIVEANGGLNQQRSAICNAVAVAGLLNAVLVIPRFEFHAIWKDSSNFGD 207

Query: 136 IYDVEKFIESLDGVVKVVKTLPSEISPR------KLSTVKVPNRVTEDYISEHVEKVFKK 195
           IYD + FI SL+G VK+V+ +P EI  R       + T++V    T +Y +  V  V K+
Sbjct: 208 IYDEDHFISSLEGYVKIVRDVPDEIMTRFSYNVSSIPTIRVQAWATVNYYNGEVYPVLKE 267

Query: 196 SGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEISEVIESMVERLKTLSRKS 255
            G IR+    P  N    +       + C+A +  L+    IS + E +V+R+   S  +
Sbjct: 268 HGVIRIT---PFANRLAMSVPPYIQLLRCIANYKALKFSSPISTLAEKLVDRMVEKSSAT 327

Query: 256 NGQFIAVDLRIE--------MLGENGCQEAS----------------------------G 315
            G++++V LR E         L E G  E S                             
Sbjct: 328 GGKYVSVHLRFEEDMVAFSCCLYEGGRAEKSEMDVIRQKSWKGKFKRRDRVIRPDLNRVN 387

Query: 316 SKSCYTAQEIALFLRKIGFNKDATIYLTQPRW---ENSLDDLKDLFPKTYTKESIMPADK 373
            K   T  E+ + LR +GF+ + +IYL   R    E  L  L+++FP+ YTKES+   ++
Sbjct: 388 GKCPLTPLEVGMMLRGMGFDNNTSIYLASGRIYQPEKHLAPLQEMFPRLYTKESLATPEE 447

BLAST of Cp4.1LG12g09550 vs. TAIR 10
Match: AT1G38065.1 (O-fucosyltransferase family protein )

HSP 1 Score: 116.7 bits (291), Expect = 4.4e-26
Identity = 98/343 (28.57%), Postives = 148/343 (43.15%), Query Frame = 0

Query: 76  ESDEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPDIRGKEL-GDKWSFGD 135
           ES       GY+      G     S I +AV VA  L A LV+P      +  D  +FGD
Sbjct: 29  ESSALAPSSGYLIVEANGGLNQQRSAICNAVAVAGLLNAVLVIPRFEFHAIWKDSSNFGD 88

Query: 136 IYDVEKFIESLDGVVKVVKTLPSEISPR------KLSTVKVPNRVTEDYISEHVEKVFKK 195
           IYD + FI SL+G VK+V+ +P EI  R       + T++V    T +Y +  V  V K+
Sbjct: 89  IYDEDHFISSLEGYVKIVRDVPDEIMTRFSYNVSSIPTIRVQAWATVNYYNGEVYPVLKE 148

Query: 196 SGSIRLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEISEVIESMVERLKTLSRKS 255
            G IR+    P  N    +       + C+A +  L+    IS + E +V+R+   S  +
Sbjct: 149 HGVIRIT---PFANRLAMSVPPYIQLLRCIANYKALKFSSPISTLAEKLVDRMVEKSSAT 208

Query: 256 NGQFIAVDLRIE--------MLGENGCQEAS----------------------------G 315
            G++++V LR E         L E G  E S                             
Sbjct: 209 GGKYVSVHLRFEEDMVAFSCCLYEGGRAEKSEMDVIRQKSWKGKFKRRDRVIRPDLNRVN 268

Query: 316 SKSCYTAQEIALFLRKIGFNKDATIYLTQPRW---ENSLDDLKDLFPKTYTKESIMPADK 373
            K   T  E+ + LR +GF+ + +IYL   R    E  L  L+++FP+ YTKES+   ++
Sbjct: 269 GKCPLTPLEVGMMLRGMGFDNNTSIYLASGRIYQPEKHLAPLQEMFPRLYTKESLATPEE 328

BLAST of Cp4.1LG12g09550 vs. TAIR 10
Match: AT4G38390.1 (root hair specific 17 )

HSP 1 Score: 111.7 bits (278), Expect = 1.4e-24
Identity = 96/344 (27.91%), Postives = 153/344 (44.48%), Query Frame = 0

Query: 77  SDEGRQPKGYVTFSLTDGPEYHVSQIADAVVVARYLGATLVVPDIRGKEL-GDKWSFGDI 136
           +D   Q   Y+  + + G     + I DAVV A  L ATLVVP +  K    D  +F DI
Sbjct: 117 TDTRSQTNRYLLIATSGGLNQQRTGIIDAVVAAYILNATLVVPKLDQKSYWKDTSNFEDI 176

Query: 137 YDVEKFIESLDGVVKVVKTLPSEISPR---KLSTVKVPNRVTEDYISEHVEKVFKKSGSI 196
           +DV+ FI  L   VK++K LP E   R    L +++VP + T     + V  +  K   +
Sbjct: 177 FDVDWFISHLSKDVKIIKELPKEEQSRISTSLQSMRVPRKCTPSCYLQRVLPILTKKHVV 236

Query: 197 RLATYFPSVNMKKSTTSSDGDSVPCLAMFGTLELQPEISEVIESMVERLKTLSRKSNGQF 256
           +L+ +   ++   +   ++   + C   +  +     I+ + + +V+R+    RK    F
Sbjct: 237 QLSKFDYRLS---NALDTELQKLRCRVNYHAVRYTESINRMGQLLVDRM----RKKAKHF 296

Query: 257 IAVDLRIE--MLGENGCQEASGSKS---------------------------C-YTAQEI 316
           +A+ LR E  ML  +GC    G K                            C  T +EI
Sbjct: 297 VALHLRFEPDMLAFSGCYYGGGQKERLELGAMRRRWKTLHAANPEKVRRHGRCPLTPEEI 356

Query: 317 ALFLRKIGFNKDATIYLTQPR---WENSLDDLKDLFPKTYTKESIMPADKKAKFLNAESS 376
            L LR +GF K+  +Y+        E++L  L+ LFP  +TKE++    + A F N  S 
Sbjct: 357 GLMLRGLGFGKEVHLYVASGEVYGGEDTLAPLRALFPNLHTKETLTSKKELAPFANFSSR 416

Query: 377 EYEKVIDFYICSQSDIFVPAISGLFYSNVAGKRIGLGKNQILVP 384
                +DF +C +SD FV   +G     +AG+R  LG    + P
Sbjct: 417 --MAALDFIVCDKSDAFVTNNNGNMARILAGRRRYLGHKVTIRP 451

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0WPA53.6e-15062.65Protein MANNAN SYNTHESIS-RELATED 2 OS=Arabidopsis thaliana OX=3702 GN=MSR2 PE=2 ... [more]
Q9LU401.4e-14662.50Protein MANNAN SYNTHESIS-RELATED 1 OS=Arabidopsis thaliana OX=3702 GN=MSR1 PE=2 ... [more]
K7R4D41.9e-11450.48Protein MANNAN SYNTHESIS-RELATED OS=Trigonella foenum-graecum OX=78534 GN=MSR PE... [more]
Q8LNP36.2e-2528.57O-fucosyltransferase 10 OS=Arabidopsis thaliana OX=3702 GN=OFUT10 PE=2 SV=2[more]
Q8GUM06.2e-2528.57O-fucosyltransferase 11 OS=Arabidopsis thaliana OX=3702 GN=OFUT11 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023548673.11.09e-298100.00protein MANNAN SYNTHESIS-RELATED 2 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023548672.17.67e-29799.76protein MANNAN SYNTHESIS-RELATED 2 isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG6575401.17.08e-29598.55Protein MANNAN SYNTHESIS-RELATED 2, partial [Cucurbita argyrosperma subsp. soror... [more]
KAG7013939.11.01e-29498.31hypothetical protein SDJN02_24108 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022992265.14.77e-29398.06protein MANNAN SYNTHESIS-RELATED 2 isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1JV822.31e-29398.06O-fucosyltransferase family protein OS=Cucurbita maxima OX=3661 GN=LOC111488638 ... [more]
A0A6J1GQV14.66e-29397.82O-fucosyltransferase family protein OS=Cucurbita moschata OX=3662 GN=LOC11145627... [more]
A0A6J1JX305.65e-29297.83O-fucosyltransferase family protein OS=Cucurbita maxima OX=3661 GN=LOC111488638 ... [more]
A0A6J1GPH23.27e-29197.58O-fucosyltransferase family protein OS=Cucurbita moschata OX=3662 GN=LOC11145627... [more]
A0A6J1KJH26.09e-27390.80O-fucosyltransferase family protein OS=Cucurbita maxima OX=3661 GN=LOC111494558 ... [more]
Match NameE-valueIdentityDescription
AT1G51630.12.6e-15162.65O-fucosyltransferase family protein [more]
AT3G21190.11.0e-14762.50O-fucosyltransferase family protein [more]
AT1G38131.14.4e-2628.57O-fucosyltransferase family protein [more]
AT1G38065.14.4e-2628.57O-fucosyltransferase family protein [more]
AT4G38390.11.4e-2427.91root hair specific 17 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.11350coord: 221..380
e-value: 2.4E-7
score: 32.7
NoneNo IPR availablePANTHERPTHR31288:SF5PROTEIN MANNAN SYNTHESIS-RELATED 1coord: 1..413
IPR024709Putative O-fucosyltransferase, plantPIRSFPIRSF009360UCP009360coord: 275..387
e-value: 2.3E-23
score: 80.7
coord: 3..277
e-value: 3.8E-54
score: 182.3
IPR024709Putative O-fucosyltransferase, plantPANTHERPTHR31288FAMILY NOT NAMEDcoord: 1..413
IPR024709Putative O-fucosyltransferase, plantCDDcd11299O-FucT_plantcoord: 86..380
e-value: 3.01454E-120
score: 349.171
IPR019378GDP-fucose protein O-fucosyltransferasePFAMPF10250O-FucTcoord: 100..372
e-value: 3.2E-29
score: 102.7

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG12g09550.1Cp4.1LG12g09550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006004 fucose metabolic process
molecular_function GO:0016757 glycosyltransferase activity