Cp4.1LG12g09090 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG12g09090
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Midasin
Location	Cp4.1LG12: 6013783 .. 6014661 (-)
RNA-Seq Expression	Cp4.1LG12g09090
Synteny	Cp4.1LG12g09090

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGACCAAATTGACGGTAAGATGTTGATTGGAAGTTACGTTTGTGCGGAACGTCCTGGTGAGTTCAGATGGCAGCCAGGTTCACTTACACAGGTATGTTTTGTTATCAAGATACATTCATTCTTCTTTTCCTATATGTTTGTCATTTCAACTCTTCTGCTTTTTTTATTTTTATTGTTTTCCAGGCTGTTCAGAATGGACTCTGGGTTGTTTTTGAGGATGTAGACAAAGCGCCATCCGATGTGCAATCCATTTTATTGCCATTATTAGAAGGTGGAAACACATTTTCAACTGGGCATGGAGAGGTATTTATTTTTTTCCCTTTTCCATTGATTTCGATATTCATGCTGAAAATATGATTGCAGTCCTTATTGTTATTTTCATTTTTTGTTCCGTAAATGGGTAAAATTTACATGAGTGTATCATAAGATCCTTGGATTTAGTTTGAATGAGAAATAGTACTTCAAGGACCTTTTTTTCCTTCTCCAGAGATGGGCAGTTATAATCTAAGGGGTGATTGAATGCAATGAAACTAAAAATGTCTTATGGACAAGTGTTTAAATAAATTTTGGGCTTTATGTGCGGGTTGGTTTCTTTTAAGCTTTGAACACTCTTTGCAACCTAAAATTTTGCCCCCCCCCCACCCCGAAAAAAAAGGAACAAATCAGTTCCGTCCAAACTAACTTTGAATTGTAGGGTAATATGAGCCAGCTTTGCCTTATAAAGATTCGACCTATACTGTTTTATGCAATCGAATACTTGTTTTATAACTCAATCTGGTTCAGGTTTTTGAATTTTTTGTGTAATTAGTGCTGTAGTGTCTTTGTGATGTTTCTTGGCTAGGTCTTGGTTATCTTGAGGTTCTTTAAACAGTAG

mRNA sequence

ATGGATGACCAAATTGACGGTAAGATGTTGATTGGAAGTTACGTTTGTGCGGAACGTCCTGGTGAGTTCAGATGGCAGCCAGGTTCACTTACACAGGCTGTTCAGAATGGACTCTGGGTTGTTTTTGAGGATGTAGACAAAGCGCCATCCGATGTGCAATCCATTTTATTGCCATTATTAGAAGGTGGAAACACATTTTCAACTGGGCATGGAGAGGTCTTGGTTATCTTGAGGTTCTTTAAACAGTAG

Coding sequence (CDS)

ATGGATGACCAAATTGACGGTAAGATGTTGATTGGAAGTTACGTTTGTGCGGAACGTCCTGGTGAGTTCAGATGGCAGCCAGGTTCACTTACACAGGCTGTTCAGAATGGACTCTGGGTTGTTTTTGAGGATGTAGACAAAGCGCCATCCGATGTGCAATCCATTTTATTGCCATTATTAGAAGGTGGAAACACATTTTCAACTGGGCATGGAGAGGTCTTGGTTATCTTGAGGTTCTTTAAACAGTAG

Protein sequence

MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLLEGGNTFSTGHGEVLVILRFFKQ

Homology

[Close]

BLAST of Cp4.1LG12g09090 vs. ExPASy Swiss-Prot
Match: A0A1P8AUY4 (Midasin OS=Arabidopsis thaliana OX=3702 GN=MDN1 PE=1 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 2.1e-25
Identity = 50/81 (61.73%), Postives = 61/81 (75.31%), Query Frame = 0


Query: 1   MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 60
           MDDQ+DGK L+G+YVC ++PGEFRWQPGSLTQA+ NG WVV ED+DKAPSDV  +L  LL
Sbjct: 386 MDDQLDGKTLVGTYVCTDQPGEFRWQPGSLTQAIMNGFWVVLEDIDKAPSDVPLVLSSLL 445

Query: 61  EGGNTFSTGHGEVLVILRFFK 82
            G  +F T  GE + I   F+
Sbjct: 446 GGSCSFLTSQGEEIRIAETFQ 466

[Close]

BLAST of Cp4.1LG12g09090 vs. ExPASy Swiss-Prot
Match: Q869L3 (Midasin OS=Dictyostelium discoideum OX=44689 GN=mdn1 PE=3 SV=2)

HSP 1 Score: 97.1 bits (240), Expect = 1.0e-19
Identity = 39/74 (52.70%), Postives = 55/74 (74.32%), Query Frame = 0


Query: 1   MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 60
           + DQ D K+L+G+Y+ ++ PGEF+WQ G+LTQAVQ G W++ ED+D AP +V S+L+PLL
Sbjct: 329 LGDQTDSKVLLGTYITSDTPGEFKWQAGALTQAVQEGRWILIEDIDLAPIEVLSVLIPLL 388

Query: 61  EGGNTFSTGHGEVL 75
           E    F  G GEV+
Sbjct: 389 ENRTLFIPGRGEVI 402

[Close]

BLAST of Cp4.1LG12g09090 vs. ExPASy Swiss-Prot
Match: Q9NU22 (Midasin OS=Homo sapiens OX=9606 GN=MDN1 PE=1 SV=2)

HSP 1 Score: 95.1 bits (235), Expect = 3.9e-19
Identity = 41/81 (50.62%), Postives = 53/81 (65.43%), Query Frame = 0


Query: 1   MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 60
           + DQ D KML+G Y C + PGEF WQPG+LTQA   G W++ ED+D AP DV S+L+PLL
Sbjct: 359 LGDQTDSKMLLGMYRCTDVPGEFVWQPGTLTQAATMGHWILLEDIDYAPLDVVSVLIPLL 418

Query: 61  EGGNTFSTGHGEVLVILRFFK 82
           E G     G G+ L +   F+
Sbjct: 419 ENGELLIPGRGDCLKVAPGFQ 439

[Close]

BLAST of Cp4.1LG12g09090 vs. ExPASy Swiss-Prot
Match: O94248 (Midasin OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mdn1 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 2.6e-15
Identity = 37/74 (50.00%), Postives = 49/74 (66.22%), Query Frame = 0


Query: 1   MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 60
           + D  D KMLIG+Y  + +PGEF WQPG LTQAV  G W++F +++ APS+V S+LLPLL
Sbjct: 185 LSDSTDPKMLIGTYT-SPKPGEFEWQPGVLTQAVITGKWILFTNIEHAPSEVLSVLLPLL 244

Query: 61  EGGNTFSTGHGEVL 75
           E         GE +
Sbjct: 245 EKRQLVIPSRGETI 257

[Close]

BLAST of Cp4.1LG12g09090 vs. ExPASy Swiss-Prot
Match: Q12019 (Midasin OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MDN1 PE=1 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 9.8e-15
Identity = 35/74 (47.30%), Postives = 47/74 (63.51%), Query Frame = 0


Query: 1   MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 60
           + +Q D K+LIG+Y   ++PG F W+ G L  AV+ G WV+ ED+DKAP+DV SILL LL
Sbjct: 343 LGEQTDAKLLIGTYTSGDKPGTFEWRAGVLATAVKEGRWVLIEDIDKAPTDVLSILLSLL 402

Query: 61  EGGNTFSTGHGEVL 75
           E         GE +
Sbjct: 403 EKRELTIPSRGETV 416

[Close]

BLAST of Cp4.1LG12g09090 vs. NCBI nr
Match: XP_022992043.1 (midasin [Cucurbita maxima] >XP_022992044.1 midasin [Cucurbita maxima])

HSP 1 Score: 155 bits (393), Expect = 7.86e-42
Identity = 73/81 (90.12%), Postives = 76/81 (93.83%), Query Frame = 0


Query: 1   MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 60
           MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL
Sbjct: 387 MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 446

Query: 61  EGGNTFSTGHGEVLVILRFFK 81
           EGGNTFSTGHGE + +   F+
Sbjct: 447 EGGNTFSTGHGEEVRVAENFR 467

[Close]

BLAST of Cp4.1LG12g09090 vs. NCBI nr
Match: XP_023548564.1 (midasin [Cucurbita pepo subsp. pepo])

HSP 1 Score: 155 bits (393), Expect = 7.86e-42
Identity = 73/81 (90.12%), Postives = 76/81 (93.83%), Query Frame = 0


Query: 1   MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 60
           MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL
Sbjct: 387 MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 446

Query: 61  EGGNTFSTGHGEVLVILRFFK 81
           EGGNTFSTGHGE + +   F+
Sbjct: 447 EGGNTFSTGHGEEVRVAENFR 467

[Close]

BLAST of Cp4.1LG12g09090 vs. NCBI nr
Match: KAG7014001.1 (Midasin [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 155 bits (393), Expect = 7.86e-42
Identity = 73/81 (90.12%), Postives = 76/81 (93.83%), Query Frame = 0


Query: 1   MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 60
           MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL
Sbjct: 387 MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 446

Query: 61  EGGNTFSTGHGEVLVILRFFK 81
           EGGNTFSTGHGE + +   F+
Sbjct: 447 EGGNTFSTGHGEEVRVAENFR 467

[Close]

BLAST of Cp4.1LG12g09090 vs. NCBI nr
Match: KAG6575456.1 (Midasin, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 155 bits (393), Expect = 7.86e-42
Identity = 73/81 (90.12%), Postives = 76/81 (93.83%), Query Frame = 0


Query: 1   MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 60
           MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL
Sbjct: 387 MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 446

Query: 61  EGGNTFSTGHGEVLVILRFFK 81
           EGGNTFSTGHGE + +   F+
Sbjct: 447 EGGNTFSTGHGEEVRVAENFR 467

[Close]

BLAST of Cp4.1LG12g09090 vs. NCBI nr
Match: XP_022953584.1 (midasin [Cucurbita moschata])

HSP 1 Score: 155 bits (393), Expect = 7.86e-42
Identity = 73/81 (90.12%), Postives = 76/81 (93.83%), Query Frame = 0


Query: 1   MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 60
           MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL
Sbjct: 387 MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 446

Query: 61  EGGNTFSTGHGEVLVILRFFK 81
           EGGNTFSTGHGE + +   F+
Sbjct: 447 EGGNTFSTGHGEEVRVAENFR 467

[Close]

BLAST of Cp4.1LG12g09090 vs. ExPASy TrEMBL
Match: A0A6J1JXY2 (Midasin OS=Cucurbita maxima OX=3661 GN=LOC111488513 PE=3 SV=1)

HSP 1 Score: 155 bits (393), Expect = 3.81e-42
Identity = 73/81 (90.12%), Postives = 76/81 (93.83%), Query Frame = 0


Query: 1   MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 60
           MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL
Sbjct: 387 MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 446

Query: 61  EGGNTFSTGHGEVLVILRFFK 81
           EGGNTFSTGHGE + +   F+
Sbjct: 447 EGGNTFSTGHGEEVRVAENFR 467

[Close]

BLAST of Cp4.1LG12g09090 vs. ExPASy TrEMBL
Match: A0A6J1GQ37 (Midasin OS=Cucurbita moschata OX=3662 GN=LOC111456078 PE=3 SV=1)

HSP 1 Score: 155 bits (393), Expect = 3.81e-42
Identity = 73/81 (90.12%), Postives = 76/81 (93.83%), Query Frame = 0


Query: 1   MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 60
           MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL
Sbjct: 387 MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 446

Query: 61  EGGNTFSTGHGEVLVILRFFK 81
           EGGNTFSTGHGE + +   F+
Sbjct: 447 EGGNTFSTGHGEEVRVAENFR 467

[Close]

BLAST of Cp4.1LG12g09090 vs. ExPASy TrEMBL
Match: A0A0A0KCN0 (Midasin OS=Cucumis sativus OX=3659 GN=Csa_7G450640 PE=3 SV=1)

HSP 1 Score: 153 bits (386), Expect = 3.35e-41
Identity = 72/81 (88.89%), Postives = 75/81 (92.59%), Query Frame = 0


Query: 1  MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 60
          MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL
Sbjct: 1  MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 60

Query: 61 EGGNTFSTGHGEVLVILRFFK 81
          EGGNTFSTG GE + +   F+
Sbjct: 61 EGGNTFSTGRGEEIRVAENFR 81

[Close]

BLAST of Cp4.1LG12g09090 vs. ExPASy TrEMBL
Match: A0A5D3BVY6 (Midasin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001100 PE=3 SV=1)

HSP 1 Score: 152 bits (385), Expect = 4.57e-41
Identity = 72/81 (88.89%), Postives = 75/81 (92.59%), Query Frame = 0


Query: 1  MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 60
          MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL
Sbjct: 1  MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 60

Query: 61 EGGNTFSTGHGEVLVILRFFK 81
          EGGNTFSTG GE + +   F+
Sbjct: 61 EGGNTFSTGRGEEVRVAENFR 81

[Close]

BLAST of Cp4.1LG12g09090 vs. ExPASy TrEMBL
Match: A0A1S3CGN9 (Midasin OS=Cucumis melo OX=3656 GN=LOC103500710 PE=3 SV=1)

HSP 1 Score: 152 bits (385), Expect = 4.57e-41
Identity = 72/81 (88.89%), Postives = 75/81 (92.59%), Query Frame = 0


Query: 1   MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 60
           MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL
Sbjct: 385 MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 444

Query: 61  EGGNTFSTGHGEVLVILRFFK 81
           EGGNTFSTG GE + +   F+
Sbjct: 445 EGGNTFSTGRGEEVRVAENFR 465

[Close]

BLAST of Cp4.1LG12g09090 vs. TAIR 10
Match: AT1G67120.1 (ATPases;nucleotide binding;ATP binding;nucleoside-triphosphatases;transcription factor binding )

HSP 1 Score: 115.9 bits (289), Expect = 1.5e-26
Identity = 50/81 (61.73%), Postives = 61/81 (75.31%), Query Frame = 0


Query: 1   MDDQIDGKMLIGSYVCAERPGEFRWQPGSLTQAVQNGLWVVFEDVDKAPSDVQSILLPLL 60
           MDDQ+DGK L+G+YVC ++PGEFRWQPGSLTQA+ NG WVV ED+DKAPSDV  +L  LL
Sbjct: 386 MDDQLDGKTLVGTYVCTDQPGEFRWQPGSLTQAIMNGFWVVLEDIDKAPSDVPLVLSSLL 445

Query: 61  EGGNTFSTGHGEVLVILRFFK 82
            G  +F T  GE + I   F+
Sbjct: 446 GGSCSFLTSQGEEIRIAETFQ 466

The following BLAST results are available for this feature:

BLAST of Cp4.1LG12g09090 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A1P8AUY4	2.1e-25	61.73	Midasin OS=Arabidopsis thaliana OX=3702 GN=MDN1 PE=1 SV=1	[more]
Q869L3	1.0e-19	52.70	Midasin OS=Dictyostelium discoideum OX=44689 GN=mdn1 PE=3 SV=2	[more]
Q9NU22	3.9e-19	50.62	Midasin OS=Homo sapiens OX=9606 GN=MDN1 PE=1 SV=2	[more]
O94248	2.6e-15	50.00	Midasin OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mdn1...	[more]
Q12019	9.8e-15	47.30	Midasin OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MD...	[more]

BLAST of Cp4.1LG12g09090 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022992043.1	7.86e-42	90.12	midasin [Cucurbita maxima] >XP_022992044.1 midasin [Cucurbita maxima]	[more]
XP_023548564.1	7.86e-42	90.12	midasin [Cucurbita pepo subsp. pepo]	[more]
KAG7014001.1	7.86e-42	90.12	Midasin [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6575456.1	7.86e-42	90.12	Midasin, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022953584.1	7.86e-42	90.12	midasin [Cucurbita moschata]	[more]

BLAST of Cp4.1LG12g09090 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JXY2	3.81e-42	90.12	Midasin OS=Cucurbita maxima OX=3661 GN=LOC111488513 PE=3 SV=1	[more]
A0A6J1GQ37	3.81e-42	90.12	Midasin OS=Cucurbita moschata OX=3662 GN=LOC111456078 PE=3 SV=1	[more]
A0A0A0KCN0	3.35e-41	88.89	Midasin OS=Cucumis sativus OX=3659 GN=Csa_7G450640 PE=3 SV=1	[more]
A0A5D3BVY6	4.57e-41	88.89	Midasin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001100 PE=3 ...	[more]
A0A1S3CGN9	4.57e-41	88.89	Midasin OS=Cucumis melo OX=3656 GN=LOC103500710 PE=3 SV=1	[more]

BLAST of Cp4.1LG12g09090 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G67120.1	1.5e-26	61.73	ATPases;nucleotide binding;ATP binding;nucleoside-triphosphatases;transcription ...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011704	ATPase, dynein-related, AAA domain	PFAM	PF07728	AAA_5	coord: 9..62 e-value: 4.3E-9 score: 36.5
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 1..80 e-value: 7.5E-11 score: 43.8
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 2..73
None	No IPR available	PANTHER	PTHR48103	MIDASIN-RELATED	coord: 1..58

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG12g09090.1	Cp4.1LG12g09090.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000027	ribosomal large subunit assembly
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016887	ATP hydrolysis activity