Cp4.1LG12g08830 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG12g08830
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Cytochrome p450
Location	Cp4.1LG12: 6167313 .. 6172035 (+)
RNA-Seq Expression	Cp4.1LG12g08830
Synteny	Cp4.1LG12g08830

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAACCACACACAGAAAAGCCTGGCAGGCAGGGCGGCAGAGCAAATCTTTGTCCAAAATGGACAGTTTCTGGGATGTTTTGGTACTGACAGCCATTTTTTTGACCATCATTCTTCTCTATAGGAACTGCTTTAGCCTTCTCAGATCAAAGCTCTGCAGCCACCTCCCTTTGGGTTCTCTTGGCTGGCCTTTCATTGGTGAAACGCTCGAGTTCATCGCTTGCGCTTATTCGGACCGCCCCGAAGCCTTCATGGACAAGCGTCGTCGCCTGTAAGTTATATGCAAAAACCAAATGATGCTTAGTTTCTCTTATGAATGGAGGGTTCATTTTTGTATGCAGGTATGGGAAGGTGTTCAAGTCACATGTTTTTGGAAGTCCTACCATTGTTTCTACTGATGCAGAAGTTAGTAAGTTCATTCTGCAAAGTGATGCCAAGGCTTTTGTACCGTGTTATCCCAAATCTTTGACGGAATTGATGGGGAAATCTTCTATTTTGCTGATAAATGGGAGCTTGCAGAAGAGGATTCATGGTCTTGTAGGAGCTTTCTTCAAGTCTTCATATCTCAAAGCTCAAATAACTAAGGATATGGAGTGTTATGTCAAATTAGCTATGGCGAATTGGAGTGAGAACAGTCCTGTTTACCTTCAAGATGAAACCAAAAATGTCAGCTCTCTCTCTCTCTCCCTCTCTCCCTCTTTACCTGATTTGAGTGAATCATTCATGTCTAAATAACCCTTTCGAGCTTCCCCTTAAGGTTTTGTTTTAAAACGCGTATGCTAGGGAGAGGTTTCCACACCCTTATAAAGAATGTTTCGTTCTACTCCCCAACCGATATGGGATCTCACAATCCACCCCCTTTGAGGCTTAGCGTCCTTGCTGGGATTCGTTCCCTTCTCCAATCGATGTGGGACCTCCCCAATCCACCTCCTTTGGGTCTCAGTGTCCACCCCTCTTCAGGGCTCAGCCTCCTCGTTGGCACATTGCTTAGTGTCTAGCTCTGATACTATTTGTAACAACTCAAGCTCACCGCTAGTAGTTATTGTCCTCTTTACGCGTCTACTAGGGAGAGGTTTCTACACCCTTATAAAAAATGGTTCGTTCTCCTCCCTAATCGATGTGGGATCTCATAATGCACCCCCCTTCTGCGCCCAGCGTTCTCGGCACTCGTTCCCTTCTCCAATCGATGTGGCACTTCCTCAATCCACCCCCTTTTGAGTCTAGCGTCCTTGCTGGTACACTGCCTCATATCCACCCCTTTCAGGGCTCAACCTCCTCGCAGGCACATTGTCCAGTGTCTGGCTCTAATAACATTTGCAACAACCCAAGCCCACCTCTAGCAAATATTGTCCTCTTTACGCGTCTGCTAGGGATAGGTTTCTACACCCTTACAAAGAATGCTTCGTTCTCCTCCCCAACCAGGGATCTCACAATTATGTTCAAATCATATTATCCAAAGAATTTACCTATGTAATCATTTTTAGCTATCATTTTCCTGTTCATAAAGTTACTAAATGAACTCCTTTTGTCTCCAGATTTCCTTTCAAGTATTAGTAAAGATATTGATTAGTTTAAATCCTGGTCAAAGGATGGAGTTTGTCAAGCAACAGTTCCAAGAATTCATCGCTGGGCTTATGGCTTTGCCAGTGAACATACCAGGCAGCAGGCTTTACCAATCGTTGCAGGCAAGTTGGATAGTAAAAGCTTTACTATCTTCCTTCACTATCACCATTTTCCTGATGGAGCTTCCTTTTATGCTTGAATTACAGGCCAAAAGGGCCATGGTTAAACTAGTTAAGAAGATCATACAAGAAAGAAGAAGCTGTGGAAGTTCCCCAGTGGCTGTCCCACAAGATGTCATGGAAGTTTTGCTCAAAGATGAAAGTGACCTACTAACAGATGATCTTATAGCAGAGAACATGATTGATATGATGATTCCTGGGGAAGATTCAGTGCCAATCTTGATGACTCTTGCAGTAAAGTACCTCTCGGATTGCCCCGAGGCACTTCAACAGTTAACGGTACGCTACGTTCTTAACAACTCTCGATCTTTTTGGTTCGAACCACTTCGAAAGAAAATAACCTCACTTTCCTTTCCATTTTAACAGTGTCCCTATCAGCCATGTCCCTCGTAAGTTAAATTATGCAAAGTTCCTTAGAACTTTCCGTTCTTTCAAAAGTTGCAATATTATTTTTGATTTTTCATGAACGTGTCAAAACTACTCTGGAAGTAGAAATCCATTAGAATGCAGGACAAAATTGAGACTTAGAATAGTGGAAAAATGACCCAAAATAGACCTTACGTAACAGCTCAAGCCTCACAATCCACCCCCTTGGAGGCATAACGTTCTTGCTGACGCACCGCCCGGTGTCTGGCTCTGATACCATTTGTAACAGCTCAAGCCCACCGCTAGTAGATATTGTCTGCTTTGGCCCGTTATGTATCATTGTCAGCCTCACGGTTTTATCACATCAGTTGGAGAGGAGAACGAAGCATTCCTTATAAGGATGAGAAACCTCTCTCTAGTGGATTCGTTTATAACCGTGAGGCTGACAGCAATACGTAACGGACCAAAGCGGACAATATCTATTAGCGGTGGGCTTGGACTGTTACAAATTATGACCCGAGCACTGCCAGCTAAGTTAACTGCATAGATTATTGTGTATCAGTAGCAGTTTAGTGTAAAGAAAATGTTGCTGTTCTTTGAGTTGTTTGATTAGTTGTTAAACCATATGGGTGCTTGAACTGATATCAGGAGGAAAACTTGAAGATGAAAAGGCTGAAAGAGCAGGCAGGAGAGGCATTGCATTGGAGCGATTACCTATCAATGCCATTTACACAGAATGTAATGCATTAAGCTTTTGATGAACTAGAAAGAAAAGGGTAGTTTAGTTGCAGTATGAATGAACACTTTGAGTCATGGTTTGATTTGAACAGGTGATCACAGAGACATTAAGGATGGGAAACATCATAATTGGTGTCATGAGGAAGGCCATGAAAGACATGAACATAAAAGGATATCTAATACCCAAAGGCTGGTCGGTGCTTACCTACTTTAGATCCGTTCATCTTGATGACAGTCACTATCACTGCCCTTACAAGTTCAATCCATGGAGGTGGCAAGTAAGAATAGAAACCGAAACGTCTCTTTATTCTAATCGTTTCTAAATTTGTGCTCAAATGAGGATCTGTGAGATCTCACGTCGGTTGGAGGGGGGAATGAATTATTGTTTATAAGGGTGTGGAAACCTCTGTTTAGTAGACGCGTTTTAAAACCTTAAAAGGAAGTCTTTGAAGGGAAAGCTCAGAGAGGACAATATCTGCTAGTGGTGGGCTTGAGCTATTACACATGGTATCAGTGTTAGACACTGGGTGGTGTGCTAGCGAGGACGTTGGGCCCCCAAAAGGGGTGGATTGTGAGATCCCACATCGGTTAGAGAAGGGAACGAAGCATTTCTTATAAGGGTGTAGAAACCTCTCCCTAGCAGACATGTTTTAAAATCTTGAGGGGAAGCCCTTGAAGGTAAAGCTTAAAGAGAACGATATCTGTTAACGGTGGGCTTGGGCTATTACAAGTGGTATTAGAGCCAGATATCAGGCGGTGTGCTAGCGAGGACGCTGGGACCCAAGGGGGGTGGATTGTGAAATCCTCCATCGGTTGGAGAGGGGAACGAAACATTTCTTATAAGGGTGTACAAATCTCTCCCTAGAAGACACATTTTAAAATCTCTAGGGGAAGCCCTTAAAGGGAAGCCCAAAGAAGACGATATCTGCTAGTGGTGGGCTTGGGCTATTACAAATAATATCAAAGTCAGATACCGGGTAGTGTGCCAACGAGGACGCTGGACACCCAAGGGGGGTGGATTGTGAGATCTCACATCGGTTGGAGAGGGGAATGAAGCGTTCCTTATAAAGGTGTGGAAACCTCTCCCTAGCAAATACACTTTAAAACCTTGAGGGGAAGCCCGTGAAGGGAAAGTCTAAAGAAGACAATATCCACTAACGATGGGCTTAGGCTTGGGCTGTTACACATCCCAAAAACTTTATTTTGAAAAACCATGTCTCCCTAACGGCTCTGTGACTCAACAGGACAGAGACTTGAGTAGTTGTAATTTCACTCCATTTGGAGGTGGACAAAGACTATGCCCTGGACTTGACTTAGCCAGGCTTGAAGCTTCCATCTTCCTTCACCACTTCGTCACTCGCTTCAGGTACCTCGCTCGTTACATCACTTTCCCATCTCAACTCATTCACGACATTTCTAAGTGCTTAAAAAAACGTTTTTAAACACTTGAAAAGTCATTCTAAGTAGGTCCCATGTTTCGTTTTCTTTCATTACGCTCGTAGCAGCTGACTCTACTAAAACTAACCCCATAAATGGTACATTACACAGATGGGTAGCTGAGGAAGATAGAATCATAAACTTCCCCACAGTGAGGATGAAGAACAGGATGCCAGTTTGGGTGAAGCAAAGAAAGGACCCATGACAATGGCTAGGGGAGCTGCCTTTTTAGCTAAACAAAGCACAAGCTCAATTAACTTCACCAACTACATAACAACCAAGAAGAAAAACAGTTTGAACGCCCGTTGTGACTGATAAAAAGTATCACTCAACACGGTTCGTAGTTTACGTGCGTGCTTGTTACTGATTGTAAATGACGTGTGTGTAGGGTGTACCTATTTGAACTGATAGTAGCCCGTCTTAACAAGTAATGTATAATACA

mRNA sequence

AGAACCACACACAGAAAAGCCTGGCAGGCAGGGCGGCAGAGCAAATCTTTGTCCAAAATGGACAGTTTCTGGGATGTTTTGGTACTGACAGCCATTTTTTTGACCATCATTCTTCTCTATAGGAACTGCTTTAGCCTTCTCAGATCAAAGCTCTGCAGCCACCTCCCTTTGGGTTCTCTTGGCTGGCCTTTCATTGGTGAAACGCTCGAGTTCATCGCTTGCGCTTATTCGGACCGCCCCGAAGCCTTCATGGACAAGCGTCGTCGCCTGTATGGGAAGGTGTTCAAGTCACATGTTTTTGGAAGTCCTACCATTGTTTCTACTGATGCAGAAGTTAGTAAGTTCATTCTGCAAAGTGATGCCAAGGCTTTTGTACCGTGTTATCCCAAATCTTTGACGGAATTGATGGGGAAATCTTCTATTTTGCTGATAAATGGGAGCTTGCAGAAGAGGATTCATGGTCTTGTAGGAGCTTTCTTCAAGTCTTCATATCTCAAAGCTCAAATAACTAAGGATATGGAGTGTTATGTCAAATTAGCTATGGCGAATTGGAGTGAGAACAGTCCTGTTTACCTTCAAGATGAAACCAAAAATATTTCCTTTCAAGTATTAGTAAAGATATTGATTAGTTTAAATCCTGGTCAAAGGATGGAGTTTGTCAAGCAACAGTTCCAAGAATTCATCGCTGGGCTTATGGCTTTGCCAGTGAACATACCAGGCAGCAGGCTTTACCAATCGTTGCAGGCAAGTTGGATAGTAAAAGCTTTACTATCTTCCTTCACTATCACCATTTTCCTGATGGAGCTTCCTTTTATGCTTGAATTACAGGCCAAAAGGGCCATGGTTAAACTAGTTAAGAAGATCATACAAGAAAGAAGAAGCTGTGGAAGTTCCCCAGTGGCTGTCCCACAAGATGTCATGGAAGTTTTGCTCAAAGATGAAAGTGACCTACTAACAGATGATCTTATAGCAGAGAACATGATTGATATGATGATTCCTGGGGAAGATTCAGTGCCAATCTTGATGACTCTTGCAGTAAAGTACCTCTCGGATTGCCCCGAGGCACTTCAACAGTTAACGGAGGAAAACTTGAAGATGAAAAGGCTGAAAGAGCAGGCAGGAGAGGCATTGCATTGGAGCGATTACCTATCAATGCCATTTACACAGAATGTGATCACAGAGACATTAAGGATGGGAAACATCATAATTGGTGTCATGAGGAAGGCCATGAAAGACATGAACATAAAAGGATATCTAATACCCAAAGGCTGGTCGGTGCTTACCTACTTTAGATCCGTTCATCTTGATGACAGTCACTATCACTGCCCTTACAAGTTCAATCCATGGAGATGGGTAGCTGAGGAAGATAGAATCATAAACTTCCCCACAGTGAGGATGAAGAACAGGATGCCAGTTTGGGTGAAGCAAAGAAAGGACCCATGACAATGGCTAGGGGAGCTGCCTTTTTAGCTAAACAAAGCACAAGCTCAATTAACTTCACCAACTACATAACAACCAAGAAGAAAAACAGTTTGAACGCCCGTTGTGACTGATAAAAAGTATCACTCAACACGGTTCGTAGTTTACGTGCGTGCTTGTTACTGATTGTAAATGACGTGTGTGTAGGGTGTACCTATTTGAACTGATAGTAGCCCGTCTTAACAAGTAATGTATAATACA

Coding sequence (CDS)

AGAACCACACACAGAAAAGCCTGGCAGGCAGGGCGGCAGAGCAAATCTTTGTCCAAAATGGACAGTTTCTGGGATGTTTTGGTACTGACAGCCATTTTTTTGACCATCATTCTTCTCTATAGGAACTGCTTTAGCCTTCTCAGATCAAAGCTCTGCAGCCACCTCCCTTTGGGTTCTCTTGGCTGGCCTTTCATTGGTGAAACGCTCGAGTTCATCGCTTGCGCTTATTCGGACCGCCCCGAAGCCTTCATGGACAAGCGTCGTCGCCTGTATGGGAAGGTGTTCAAGTCACATGTTTTTGGAAGTCCTACCATTGTTTCTACTGATGCAGAAGTTAGTAAGTTCATTCTGCAAAGTGATGCCAAGGCTTTTGTACCGTGTTATCCCAAATCTTTGACGGAATTGATGGGGAAATCTTCTATTTTGCTGATAAATGGGAGCTTGCAGAAGAGGATTCATGGTCTTGTAGGAGCTTTCTTCAAGTCTTCATATCTCAAAGCTCAAATAACTAAGGATATGGAGTGTTATGTCAAATTAGCTATGGCGAATTGGAGTGAGAACAGTCCTGTTTACCTTCAAGATGAAACCAAAAATATTTCCTTTCAAGTATTAGTAAAGATATTGATTAGTTTAAATCCTGGTCAAAGGATGGAGTTTGTCAAGCAACAGTTCCAAGAATTCATCGCTGGGCTTATGGCTTTGCCAGTGAACATACCAGGCAGCAGGCTTTACCAATCGTTGCAGGCAAGTTGGATAGTAAAAGCTTTACTATCTTCCTTCACTATCACCATTTTCCTGATGGAGCTTCCTTTTATGCTTGAATTACAGGCCAAAAGGGCCATGGTTAAACTAGTTAAGAAGATCATACAAGAAAGAAGAAGCTGTGGAAGTTCCCCAGTGGCTGTCCCACAAGATGTCATGGAAGTTTTGCTCAAAGATGAAAGTGACCTACTAACAGATGATCTTATAGCAGAGAACATGATTGATATGATGATTCCTGGGGAAGATTCAGTGCCAATCTTGATGACTCTTGCAGTAAAGTACCTCTCGGATTGCCCCGAGGCACTTCAACAGTTAACGGAGGAAAACTTGAAGATGAAAAGGCTGAAAGAGCAGGCAGGAGAGGCATTGCATTGGAGCGATTACCTATCAATGCCATTTACACAGAATGTGATCACAGAGACATTAAGGATGGGAAACATCATAATTGGTGTCATGAGGAAGGCCATGAAAGACATGAACATAAAAGGATATCTAATACCCAAAGGCTGGTCGGTGCTTACCTACTTTAGATCCGTTCATCTTGATGACAGTCACTATCACTGCCCTTACAAGTTCAATCCATGGAGATGGGTAGCTGAGGAAGATAGAATCATAAACTTCCCCACAGTGAGGATGAAGAACAGGATGCCAGTTTGGGTGAAGCAAAGAAAGGACCCATGA

Protein sequence

RTTHRKAWQAGRQSKSLSKMDSFWDVLVLTAIFLTIILLYRNCFSLLRSKLCSHLPLGSLGWPFIGETLEFIACAYSDRPEAFMDKRRRLYGKVFKSHVFGSPTIVSTDAEVSKFILQSDAKAFVPCYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSYLKAQITKDMECYVKLAMANWSENSPVYLQDETKNISFQVLVKILISLNPGQRMEFVKQQFQEFIAGLMALPVNIPGSRLYQSLQASWIVKALLSSFTITIFLMELPFMLELQAKRAMVKLVKKIIQERRSCGSSPVAVPQDVMEVLLKDESDLLTDDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPEALQQLTEENLKMKRLKEQAGEALHWSDYLSMPFTQNVITETLRMGNIIIGVMRKAMKDMNIKGYLIPKGWSVLTYFRSVHLDDSHYHCPYKFNPWRWVAEEDRIINFPTVRMKNRMPVWVKQRKDP

Homology

[Close]

BLAST of Cp4.1LG12g08830 vs. ExPASy Swiss-Prot
Match: Q94IA6 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=3702 GN=CYP90D1 PE=1 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 3.4e-139
Identity = 265/518 (51.16%), Postives = 339/518 (65.44%), Query Frame = 0


Query: 20  MDSFWDVLVLTAIFLTIILLYRNCFSLLRS-----KLCSH----------LPLGSLGWPF 79
           MD+   +L  +  F  II+++     L  S     KL  H           P GSLGWP 
Sbjct: 1   MDTSSSLLFFSFFFFIIIVIFNKINGLRSSPASKKKLNDHHVTSQSHGPKFPHGSLGWPV 60

Query: 80  IGETLEFIACAYSDRPEAFMDKRRRLYGKVFKSHVFGSPTIVSTDAEVSKFILQSDAKAF 139
           IGET+EF++ AYSDRPE+FMDKRR +YG+VFKSH+FG+ TIVSTDAEV++ +LQSD+ AF
Sbjct: 61  IGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVNRAVLQSDSTAF 120

Query: 140 VPCYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSYLKAQITKDMECYVKLAMANW 199
           VP YPK++ ELMGKSSILLINGSL +R HGLVG+F KS  LKAQI +DM  ++  +M  W
Sbjct: 121 VPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDMHKFLSESMDLW 180

Query: 200 SENSPVYLQDETKNISFQVLVKILISLNPGQRMEFVKQQFQEFIAGLMALPVNIPGSRLY 259
           SE+ PV LQD +K ++F+VL K LIS+  G+ +E +K++F+ FI+GLM+LP+N PG++L+
Sbjct: 181 SEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFISGLMSLPINFPGTQLH 240

Query: 260 QSLQASWIVKALLSSFTITIFLMELPFMLELQAKRAMVKLVKKIIQ---ERRSCGSSPVA 319
           +S                            LQAK+ MVK V++II+    +         
Sbjct: 241 RS----------------------------LQAKKNMVKQVERIIEGKIRKTKNKEEDDV 300

Query: 320 VPQDVMEVLLKDESDLLTDDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPEALQQLTE 379
           + +DV++VLLKD S+ LT +LIA NMIDMMIPG DSVP+L+TLAVK+LSD P AL  LTE
Sbjct: 301 IAKDVVDVLLKDSSEHLTHNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTE 360

Query: 380 ENLKMKRLKEQAGEALHWSDYLSMPFTQNVITETLRMGNIIIGVMRKAMKDMNIKGYLIP 439
           EN+K+K LKE  GE L+W+DYLS+PFTQ VITETLRMGN+IIGVMRKAMKD+ IKGY+IP
Sbjct: 361 ENMKLKSLKELTGEPLYWNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIP 420

Query: 440 KGWSVLTYFRSVHLDDSHYHCPYKFNPW-------------------------------- 477
           KGW  L Y RSVHLD  +Y  PYKFNPW                                
Sbjct: 421 KGWCFLAYLRSVHLDKLYYESPYKFNPWRWQERDMNTSSFSPFGGGQRLCPGLDLARLET 480

[Close]

BLAST of Cp4.1LG12g08830 vs. ExPASy Swiss-Prot
Match: Q94IW5 (Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90D2 PE=1 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 1.2e-136
Identity = 249/473 (52.64%), Postives = 334/473 (70.61%), Query Frame = 0


Query: 53  SHLPLGSLGWPFIGETLEFIACAYSDRPEAFMDKRRRLYGK-VFKSHVFGSPTIVSTDAE 112
           + LP GS GWP +GETLEF++CAYS RPEAF+DKRR+L+G  VF+SH+FGS T+V+ DAE
Sbjct: 47  ARLPPGSFGWPVVGETLEFVSCAYSPRPEAFVDKRRKLHGSAVFRSHLFGSATVVTADAE 106

Query: 113 VSKFILQSDAKAFVPCYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSYLKAQITK 172
           VS+F+LQSDA+AFVP YP+SLTELMGKSSILLING+LQ+R+HGLVGAFFKSS+LK+Q+T 
Sbjct: 107 VSRFVLQSDARAFVPWYPRSLTELMGKSSILLINGALQRRVHGLVGAFFKSSHLKSQLTA 166

Query: 173 DMECYVKLAMANWSENSPVYLQDETKNISFQVLVKILISLNPGQRMEFVKQQFQEFIAGL 232
           DM   +  A++++ ++S +++Q   K++ F++LV+ LI L  G+ M+ +KQQFQEFI GL
Sbjct: 167 DMRRRLSPALSSFPDSSLLHVQHLAKSVVFEILVRGLIGLEAGEEMQQLKQQFQEFIVGL 226

Query: 233 MALPVNIPGSRLYQSLQASWIVKALLSSFTITIFLMELPFMLELQAKRAMVKLVKKIIQE 292
           M+LP+ +PG+RLY+S                            LQAK+ M +L+++II+E
Sbjct: 227 MSLPIKLPGTRLYRS----------------------------LQAKKKMARLIQRIIRE 286

Query: 293 RRS--CGSSPVAVPQDVMEVLLKDESDLLTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 352
           +R+    +SP   P+D ++VL+ D SD LTD+LI++NMID+MIP EDSVP+L+TLAVK+L
Sbjct: 287 KRARRAAASP---PRDAIDVLIGDGSDELTDELISDNMIDLMIPAEDSVPVLITLAVKFL 346

Query: 353 SDCPEALQQLTEENLKMKRLKEQAGEALHWSDYLSMPFTQNVITETLRMGNIIIGVMRKA 412
           S+CP AL QL EEN+++KR K   GE L W+DY+S+ FTQ+VITETLR+GNII G+MRKA
Sbjct: 347 SECPLALHQLEEENIQLKRRKTDMGETLQWTDYMSLSFTQHVITETLRLGNIIGGIMRKA 406

Query: 413 MKDMNIKGYLIPKGWSVLTYFRSVHLDDSHYHCPYKFNPW-------------------- 472
           ++D+ +KG+LIPKGW V  YFRSVHLDD+ Y  PYKFNPW                    
Sbjct: 407 VRDVEVKGHLIPKGWCVFVYFRSVHLDDTLYDEPYKFNPWRWKEKDMSNGSFTPFGGGQR 466

Query: 473 -----------------------RWVAEEDRIINFPTVRMKNRMPVWVKQRKD 480
                                  RWVAEED I+NFPTVR+K  MP+ V  ++D
Sbjct: 467 LCPGLDLARLEASIFLHHLVTSFRWVAEEDHIVNFPTVRLKRGMPIRVTAKED 488

[Close]

BLAST of Cp4.1LG12g08830 vs. ExPASy Swiss-Prot
Match: Q9M066 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 OS=Arabidopsis thaliana OX=3702 GN=CYP90C1 PE=1 SV=3)

HSP 1 Score: 425.2 bits (1092), Expect = 9.5e-118
Identity = 218/470 (46.38%), Postives = 301/470 (64.04%), Query Frame = 0


Query: 55  LPLGSLGWPFIGETLEFIACAYSDRPEAFMDKRRRLYGKVFKSHVFGSPTIVSTDAEVSK 114
           +P GSLGWP IGETL FIAC YS RP  FMDKR+ LYGKVFK+++ G+P I+STDAEV+K
Sbjct: 69  IPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNK 128

Query: 115 FILQSDAKAFVPCYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSYLKAQITKDME 174
            +LQ+    FVP YPKS+TEL+G++SIL ING  QKR+H L+GAF +S +LK +IT+D+E
Sbjct: 129 VVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIE 188

Query: 175 CYVKLAMANWSENSPVYLQDETKNISFQVLVKILISLNPGQRMEFVKQQFQEFIAGLMAL 234
             V L +A+W++   V++QDE K ++F++LVK+L+S +PG+ M  +K +F+EFI GL+ +
Sbjct: 189 ASVVLTLASWAQLPLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFIKGLICI 248

Query: 235 PVNIPGSRLYQSLQASWIVKALLSSFTITIFLMELPFMLELQAKRAMVKLVKKIIQERRS 294
           P+  PG+RLY+S                            L+AK  ++K+VKK+++ER+ 
Sbjct: 249 PIKFPGTRLYKS----------------------------LKAKERLIKMVKKVVEERQ- 308

Query: 295 CGSSPVAVPQDVMEVLLKD----ESDLLTDDLIAENMIDMMIPGEDSVPILMTLAVKYLS 354
              +  +   DV++VLL+D    E      D ++  +++MMIPGE+++P  MTLAVK+LS
Sbjct: 309 VAMTTTSPANDVVDVLLRDGGDSEKQSQPSDFVSGKIVEMMIPGEETMPTAMTLAVKFLS 368

Query: 355 DCPEALQQLTEENLKMKRLKEQAGEALHWSDYLSMPFTQNVITETLRMGNIIIGVMRKAM 414
           D P AL +L EEN++MKR K + GE   W+DY+S+ FTQNVI ETLRM NII GV RKA+
Sbjct: 369 DNPVALAKLVEENMEMKRRKLELGEEYKWTDYMSLSFTQNVINETLRMANIINGVWRKAL 428

Query: 415 KDMNIKGYLIPKGWSVLTYFRSVHLDDSHYHCPYKFNPWR-------------------- 474
           KD+ IKGYLIPKGW VL  F SVH+D+  Y  PY+F+PWR                    
Sbjct: 429 KDVEIKGYLIPKGWCVLASFISVHMDEDIYDNPYQFDPWRWDRINGSANSSICFTPFGGG 488

[Close]

BLAST of Cp4.1LG12g08830 vs. ExPASy Swiss-Prot
Match: Q42569 (Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 1.1e-76
Identity = 166/470 (35.32%), Postives = 254/470 (54.04%), Query Frame = 0


Query: 55  LPLGSLGWPFIGETLEFIACAYSDRPEAFMDKRRRLYGKVFKSHVFGSPTIVSTDAEVSK 114
           LP GSLG P IGET + I    ++ PE F+D+R   YG VF +H+FG PTI S D E ++
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 115 FILQSDAKAFVPCYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSYLKAQITKDME 174
           F+LQ++ K F   YP S+  L+GK S+LL+ GSL KR+H L  +F  SS +K  +  D++
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 175 CYVKLAMANWSENSPVYLQDETKNISFQVLVKILISLNPGQRMEFVKQQFQEFIAGLMAL 234
             V+  + +WS  S V L +E K I+F++ VK L+S +PG+  E +++++   I G  +L
Sbjct: 151 RLVRFNLDSWS--SRVLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFSL 210

Query: 235 PVNIPGSRLYQSLQASWIVKALLSSFTITIFLMELPFMLELQAKRAMVKLVKKIIQERRS 294
           P+                             L    +   +QA+R + + +  ++ +RR 
Sbjct: 211 PLP----------------------------LFSTTYRKAIQARRKVAEALTVVVMKRRE 270

Query: 295 CGSSPVAVPQDVMEVLLKDESDLLTDDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPE 354
                    +D++  LL  + D  +D+ I + ++ +++ G ++   +MTLAVK+L++ P 
Sbjct: 271 EEEEGAERKKDMLAALLAAD-DGFSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETPL 330

Query: 355 ALQQLTEENLKMKRLKEQAGEALHWSDYLSMPFTQNVITETLRMGNIIIGVMRKAMKDMN 414
           AL QL EE+ K++ +K  +  +L WSDY SMPFTQ V+ ETLR+ NII GV R+AM D+ 
Sbjct: 331 ALAQLKEEHEKIRAMKSDS-YSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVE 390

Query: 415 IKGYLIPKGWSVLTYFRSVHLDDSHYHCPYKFNPWRW----------------------- 474
           IKGY IPKGW V + FR+VHLD +H+     FNPWRW                       
Sbjct: 391 IKGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQSNSVTTGPSNVFTPFGGGPRLC 450

Query: 475 ------------------------VAEEDRIINFPTVRMKNRMPVWVKQR 478
                                    AE+D+++ FPT R + R P++VK+R
Sbjct: 451 PGYELARVALSVFLHRLVTGFSWVPAEQDKLVFFPTTRTQKRYPIFVKRR 468

[Close]

BLAST of Cp4.1LG12g08830 vs. ExPASy Swiss-Prot
Match: O64989 (Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2)

HSP 1 Score: 251.5 bits (641), Expect = 1.9e-65
Identity = 144/429 (33.57%), Postives = 246/429 (57.34%), Query Frame = 0


Query: 29  LTAIFLTIILLYRNCFSLLRSKLCSHLPLGSLGWPFIGETLEFIACAYSDRPEAFMDKRR 88
           L ++ L +ILL R        K   +LP G  GWPF+GET+ ++    +     FM +  
Sbjct: 18  LLSLLLFLILLKRR-----NRKTRFNLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHV 77

Query: 89  RLYGKVFKSHVFGSPTIVSTDAEVSKFILQSDAKAFVPCYPKSLTELMGKSSILLINGSL 148
             YGK+++S++FG PTIVS DA +++FILQ++ + F   YP+S+  ++GK S+L++ G +
Sbjct: 78  SKYGKIYRSNLFGEPTIVSADAGLNRFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDM 137

Query: 149 QKRIHGLVGAFFKSSYLKAQITKDMECYVKLAMANWSENSPVYLQDETKNISFQVLVKIL 208
            + +  +   F   + L+  + KD+E +    + +W +NS    QDE K  +F ++ K +
Sbjct: 138 HRDMRSISLNFLSHARLRTILLKDVERHTLFVLDSWQQNSIFSAQDEAKKFTFNLMAKHI 197

Query: 209 ISLNPG-QRMEFVKQQFQEFIAGLMALPVNIPGSRLYQSLQASWIVKALLSSFTITIFLM 268
           +S++PG +  E +K+++  F+ G+++ P+N+PG+  +         KAL S  TI  F+ 
Sbjct: 198 MSMDPGEEETEQLKKEYVTFMKGVVSAPLNLPGTAYH---------KALQSRATILKFIE 257

Query: 269 ELPFMLELQAKRAMVKLVKKIIQERRSCGSSPVAVPQ----DVMEVLLKDESDLLTDDLI 328
                 +L  K    +  +   ++      S     Q    D++  +LK  +  L+ + I
Sbjct: 258 RKMEERKLDIKEEDQEEEEVKTEDEAEMSKSDHVRKQRTDDDLLGWVLKHSN--LSTEQI 317

Query: 329 AENMIDMMIPGEDSVPILMTLAVKYLSDCPEALQQLTEENLKMKRLKEQAGEA-LHWSDY 388
            + ++ ++  G ++  + + LA+ +L  CP+A+++L EE+L++ R K++ GE+ L+W DY
Sbjct: 318 LDLILSLLFAGHETSSVAIALAIFFLQACPKAVEELREEHLEIARAKKELGESELNWDDY 377

Query: 389 LSMPFTQNVITETLRMGNIIIGVMRKAMKDMNIKGYLIPKGWSVLTYFRSVHLDDSHYHC 448
             M FTQ VI ETLR+GN++  + RKA+KD+  KGY IP GW VL    +VHLD+S Y  
Sbjct: 378 KKMDFTQCVINETLRLGNVVRFLHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQ 430

Query: 449 PYKFNPWRW 452
           P  FNPWRW
Sbjct: 438 PNLFNPWRW 430

[Close]

BLAST of Cp4.1LG12g08830 vs. NCBI nr
Match: KAG6575481.1 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 889 bits (2297), Expect = 0.0
Identity = 458/504 (90.87%), Postives = 460/504 (91.27%), Query Frame = 0


Query: 20  MDSFWDVLVLTAIFLTIILLYRNCFSLLRSKLCSHLPLGSLGWPFIGETLEFIACAYSDR 79
           MDSFWDVLVLTAIFLTIILLYRNCFSLLRSKLCSHLPLGSLGWPFIGETLEFIACAYSDR
Sbjct: 1   MDSFWDVLVLTAIFLTIILLYRNCFSLLRSKLCSHLPLGSLGWPFIGETLEFIACAYSDR 60

Query: 80  PEAFMDKRRRLYGKVFKSHVFGSPTIVSTDAEVSKFILQSDAKAFVPCYPKSLTELMGKS 139
           PEAFMDKRRRLYGKVFKSHVFGSPTIVSTDAEVSKFILQSDAKAFVPCYPKSLTELMGKS
Sbjct: 61  PEAFMDKRRRLYGKVFKSHVFGSPTIVSTDAEVSKFILQSDAKAFVPCYPKSLTELMGKS 120

Query: 140 SILLINGSLQKRIHGLVGAFFKSSYLKAQITKDMECYVKLAMANWSENSPVYLQDETKNI 199
           SILLINGSLQKRIHGLVGAFFKSSYLKAQITKDMECYVKLAMANWSENSPVYLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSYLKAQITKDMECYVKLAMANWSENSPVYLQDETKNI 180

Query: 200 SFQVLVKILISLNPGQRMEFVKQQFQEFIAGLMALPVNIPGSRLYQSLQASWIVKALLSS 259
           SFQVLVKILISLNPGQRMEFVKQQFQEFIAGLMALPVNIPGSRLYQSLQASWIVKAL SS
Sbjct: 181 SFQVLVKILISLNPGQRMEFVKQQFQEFIAGLMALPVNIPGSRLYQSLQASWIVKALPSS 240

Query: 260 FTITIFLMELPFMLELQAKRAMVKLVKKIIQERRSCGSSPVAVPQDVMEVLLKDESDLLT 319
           FTITIFLMELPFMLELQAKRAMVKLVKKIIQERRSCGSSPVAVPQDVMEVLLKDES+LLT
Sbjct: 241 FTITIFLMELPFMLELQAKRAMVKLVKKIIQERRSCGSSPVAVPQDVMEVLLKDESELLT 300

Query: 320 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPEALQQLTEENLKMKRLKEQAGEALHW 379
           DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPEALQQLTEENLKMKRLKEQAGEALHW
Sbjct: 301 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPEALQQLTEENLKMKRLKEQAGEALHW 360

Query: 380 SDYLSMPFTQNVITETLRMGNIIIGVMRKAMKDMNIKGYLIPKGWSVLTYFRSVHLDDSH 439
           SDYLSMPFTQNVITETLRMGNIIIGVMRKAMKDMNIKGYLIPKGWSVLTYFRSVHLDDSH
Sbjct: 361 SDYLSMPFTQNVITETLRMGNIIIGVMRKAMKDMNIKGYLIPKGWSVLTYFRSVHLDDSH 420

Query: 440 YHCPYKFNPWRW-------------------------------------------VAEED 480
           YHCPYKFNPWRW                                           VAEED
Sbjct: 421 YHCPYKFNPWRWQDRDLSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTRFRWVAEED 480

[Close]

BLAST of Cp4.1LG12g08830 vs. NCBI nr
Match: XP_008462361.1 (PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase [Cucumis melo])

HSP 1 Score: 735 bits (1898), Expect = 5.50e-264
Identity = 384/504 (76.19%), Postives = 408/504 (80.95%), Query Frame = 0


Query: 20  MDSFWDVLVLTAIFLTIILLYRNCFSLLRSKLCSHLPLGSLGWPFIGETLEFIACAYSDR 79
           MDS WDVLV+TAI    I+LYRNCF LLRSK C+ LPLGSLGWPFIGET+EFI+CAYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 80  PEAFMDKRRRLYGKVFKSHVFGSPTIVSTDAEVSKFILQSDAKAFVPCYPKSLTELMGKS 139
           PE FMDKRRRLYGKVFKSH+FGSPTIVSTDAEVSKFILQSDAKAFVP YPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 140 SILLINGSLQKRIHGLVGAFFKSSYLKAQITKDMECYVKLAMANWSENSPVYLQDETKNI 199
           SILLINGSLQKRIHGLVGAFFKSS+LKAQITKDMECYVKL+MA+W+EN+P+YLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 200 SFQVLVKILISLNPGQRMEFVKQQFQEFIAGLMALPVNIPGSRLYQSLQASWIVKALLSS 259
           SFQVLVKILISLNPGQ MEF+K+QFQEFIAGLMALPVNIPGSRLYQSLQA          
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQA---------- 240

Query: 260 FTITIFLMELPFMLELQAKRAMVKLVKKIIQERRSCGSSPVAVPQDVMEVLLKDESDLLT 319
                             KR MVKLVKKIIQER+SCG S   VP+DVMEVLLKDE++ LT
Sbjct: 241 ------------------KRNMVKLVKKIIQERKSCGIS--TVPKDVMEVLLKDENEELT 300

Query: 320 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPEALQQLTEENLKMKRLKEQAGEALHW 379
           DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCP ALQQLTEENLKMK  KEQ GEALHW
Sbjct: 301 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKGQKEQLGEALHW 360

Query: 380 SDYLSMPFTQNVITETLRMGNIIIGVMRKAMKDMNIKGYLIPKGWSVLTYFRSVHLDDSH 439
           SDYLSMPFTQ+VITETLRMGNIIIGVMRKAMKDM IKGYLI KGWSVLTYFRSVHLDD+H
Sbjct: 361 SDYLSMPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLILKGWSVLTYFRSVHLDDNH 420

Query: 440 YHCPYKFNPWRW-------------------------------------------VAEED 480
           Y CPYKFNPWRW                                           +AEED
Sbjct: 421 YDCPYKFNPWRWQERELSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTHFRWIAEED 471

[Close]

BLAST of Cp4.1LG12g08830 vs. NCBI nr
Match: KAA0059438.1 (cytochrome p450 [Cucumis melo var. makuwa])

HSP 1 Score: 717 bits (1851), Expect = 1.66e-253
Identity = 367/442 (83.03%), Postives = 387/442 (87.56%), Query Frame = 0


Query: 20  MDSFWDVLVLTAIFLTIILLYRNCFSLLRSKLCSHLPLGSLGWPFIGETLEFIACAYSDR 79
           MDS WDVLV+TAI    I+LYRNCF LLRSK C+ LPLGSLGWPFIGET+EFI+CAYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 80  PEAFMDKRRRLYGKVFKSHVFGSPTIVSTDAEVSKFILQSDAKAFVPCYPKSLTELMGKS 139
           PE FMDKRRRLYGKVFKSH+FGSPTIVSTDAEVSKFILQSDAKAFVP YPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 140 SILLINGSLQKRIHGLVGAFFKSSYLKAQITKDMECYVKLAMANWSENSPVYLQDETKNI 199
           SILLINGSLQKRIHGLVGAFFKSS+LKAQITKDMECYVKL+MA+W+EN+P+YLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 200 SFQVLVKILISLNPGQRMEFVKQQFQEFIAGLMALPVNIPGSRLYQSLQASWIVKALLSS 259
           SFQVLVKILISLNPGQ MEF+K+QFQEFIAGLMALPVNIPGSRLYQSLQASW        
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQASW-------- 240

Query: 260 FTITIFLMELPFMLELQAKRAMVKLVKKIIQERRSCGSSPVAVPQDVMEVLLKDESDLLT 319
                            AKR MVKLVKKIIQER+SCG S   VP+DVMEVLLKDE++ LT
Sbjct: 241 ----------------TAKRNMVKLVKKIIQERKSCGIS--TVPKDVMEVLLKDENEELT 300

Query: 320 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPEALQQLTEENLKMKRLKEQAGEALHW 379
           DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCP ALQQLTEENLKMK  KEQ GEALHW
Sbjct: 301 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKGQKEQLGEALHW 360

Query: 380 SDYLSMPFTQNVITETLRMGNIIIGVMRKAMKDMNIKGYLIPKGWSVLTYFRSVHLDDSH 439
           SDYLSMPFTQ+VITETLRMGNIIIGVMRKAMKDM IKGYLIPKGWSVLTYFRSVHLDD+H
Sbjct: 361 SDYLSMPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNH 413

Query: 440 YHCPYKFNPWRWVAEEDRIINF 461
           Y CPYKFNPWRW   E    NF
Sbjct: 421 YDCPYKFNPWRWQERELSSCNF 413

[Close]

BLAST of Cp4.1LG12g08830 vs. NCBI nr
Match: XP_011659645.1 (LOW QUALITY PROTEIN: 3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 [Cucumis sativus])

HSP 1 Score: 715 bits (1846), Expect = 8.56e-253
Identity = 365/442 (82.58%), Postives = 385/442 (87.10%), Query Frame = 0


Query: 20  MDSFWDVLVLTAIFLTIILLYRNCFSLLRSKLCSHLPLGSLGWPFIGETLEFIACAYSDR 79
           MDS WDVLV+TAIF T I+LYRNCF LLRSK C+ LPLGSLGWPFIGET+EFI+ AYSDR
Sbjct: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60

Query: 80  PEAFMDKRRRLYGKVFKSHVFGSPTIVSTDAEVSKFILQSDAKAFVPCYPKSLTELMGKS 139
           PE FMDKRRRLYGKVFKSH+FGSPTIVSTDAEVSKFILQSDAKAFVP YPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 140 SILLINGSLQKRIHGLVGAFFKSSYLKAQITKDMECYVKLAMANWSENSPVYLQDETKNI 199
           SILLINGSLQKRIHGLVGAFFKSS+LKAQITKDMECYVKL+MANW+EN+P+YLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180

Query: 200 SFQVLVKILISLNPGQRMEFVKQQFQEFIAGLMALPVNIPGSRLYQSLQASWIVKALLSS 259
           SFQVLVKILISLNPGQ MEF+K+QFQEFIAGLMALPVNIPGSRLYQSLQA          
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQA---------- 240

Query: 260 FTITIFLMELPFMLELQAKRAMVKLVKKIIQERRSCGSSPVAVPQDVMEVLLKDESDLLT 319
                             KR MVKLVKKIIQER+SCG S   VP+D+MEVLLKDE++ LT
Sbjct: 241 ------------------KRNMVKLVKKIIQERKSCGIS--TVPKDIMEVLLKDENEELT 300

Query: 320 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPEALQQLTEENLKMKRLKEQAGEALHW 379
           DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCP ALQQLTEENLKMK  KEQ GEALHW
Sbjct: 301 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKGQKEQLGEALHW 360

Query: 380 SDYLSMPFTQNVITETLRMGNIIIGVMRKAMKDMNIKGYLIPKGWSVLTYFRSVHLDDSH 439
           SDYLSMPFTQ+VITETLRMGNIIIGVMRKAMKDM IKGYLIPKGWSVLTYFRSVHLDD+H
Sbjct: 361 SDYLSMPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNH 412

Query: 440 YHCPYKFNPWRWVAEEDRIINF 461
           Y CPYKFNPWRW   E    NF
Sbjct: 421 YDCPYKFNPWRWQDREMSSCNF 412

[Close]

BLAST of Cp4.1LG12g08830 vs. NCBI nr
Match: KAE8646515.1 (hypothetical protein Csa_016429 [Cucumis sativus])

HSP 1 Score: 715 bits (1846), Expect = 1.13e-252
Identity = 365/442 (82.58%), Postives = 385/442 (87.10%), Query Frame = 0


Query: 20  MDSFWDVLVLTAIFLTIILLYRNCFSLLRSKLCSHLPLGSLGWPFIGETLEFIACAYSDR 79
           MDS WDVLV+TAIF T I+LYRNCF LLRSK C+ LPLGSLGWPFIGET+EFI+ AYSDR
Sbjct: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60

Query: 80  PEAFMDKRRRLYGKVFKSHVFGSPTIVSTDAEVSKFILQSDAKAFVPCYPKSLTELMGKS 139
           PE FMDKRRRLYGKVFKSH+FGSPTIVSTDAEVSKFILQSDAKAFVP YPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 140 SILLINGSLQKRIHGLVGAFFKSSYLKAQITKDMECYVKLAMANWSENSPVYLQDETKNI 199
           SILLINGSLQKRIHGLVGAFFKSS+LKAQITKDMECYVKL+MANW+EN+P+YLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180

Query: 200 SFQVLVKILISLNPGQRMEFVKQQFQEFIAGLMALPVNIPGSRLYQSLQASWIVKALLSS 259
           SFQVLVKILISLNPGQ MEF+K+QFQEFIAGLMALPVNIPGSRLYQSLQA          
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQA---------- 240

Query: 260 FTITIFLMELPFMLELQAKRAMVKLVKKIIQERRSCGSSPVAVPQDVMEVLLKDESDLLT 319
                             KR MVKLVKKIIQER+SCG S   VP+D+MEVLLKDE++ LT
Sbjct: 241 ------------------KRNMVKLVKKIIQERKSCGIS--TVPKDIMEVLLKDENEELT 300

Query: 320 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPEALQQLTEENLKMKRLKEQAGEALHW 379
           DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCP ALQQLTEENLKMK  KEQ GEALHW
Sbjct: 301 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKGQKEQLGEALHW 360

Query: 380 SDYLSMPFTQNVITETLRMGNIIIGVMRKAMKDMNIKGYLIPKGWSVLTYFRSVHLDDSH 439
           SDYLSMPFTQ+VITETLRMGNIIIGVMRKAMKDM IKGYLIPKGWSVLTYFRSVHLDD+H
Sbjct: 361 SDYLSMPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNH 412

Query: 440 YHCPYKFNPWRWVAEEDRIINF 461
           Y CPYKFNPWRW   E    NF
Sbjct: 421 YDCPYKFNPWRWQDREMSSCNF 412

[Close]

BLAST of Cp4.1LG12g08830 vs. ExPASy TrEMBL
Match: A0A0A0K7W8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G451390 PE=3 SV=1)

HSP 1 Score: 746 bits (1925), Expect = 2.31e-268
Identity = 386/504 (76.59%), Postives = 410/504 (81.35%), Query Frame = 0


Query: 20  MDSFWDVLVLTAIFLTIILLYRNCFSLLRSKLCSHLPLGSLGWPFIGETLEFIACAYSDR 79
           MDS WDVLV+TAIF T I+LYRNCF LLRSK C+ LPLGSLGWPFIGET+EFI+ AYSDR
Sbjct: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60

Query: 80  PEAFMDKRRRLYGKVFKSHVFGSPTIVSTDAEVSKFILQSDAKAFVPCYPKSLTELMGKS 139
           PE FMDKRRRLYGKVFKSH+FGSPTIVSTDAEVSKFILQSDAKAFVP YPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 140 SILLINGSLQKRIHGLVGAFFKSSYLKAQITKDMECYVKLAMANWSENSPVYLQDETKNI 199
           SILLINGSLQKRIHGLVGAFFKSS+LKAQITKDMECYVKL+MANW+EN+P+YLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180

Query: 200 SFQVLVKILISLNPGQRMEFVKQQFQEFIAGLMALPVNIPGSRLYQSLQASWIVKALLSS 259
           SFQVLVKILISLNPGQ MEF+K+QFQEFIAGLMALPVNIPGSRLYQSLQA          
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQA---------- 240

Query: 260 FTITIFLMELPFMLELQAKRAMVKLVKKIIQERRSCGSSPVAVPQDVMEVLLKDESDLLT 319
                             KR MVKLVKKIIQER+SCG S   VP+D+MEVLLKDE++ LT
Sbjct: 241 ------------------KRNMVKLVKKIIQERKSCGIS--TVPKDIMEVLLKDENEELT 300

Query: 320 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPEALQQLTEENLKMKRLKEQAGEALHW 379
           DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCP ALQQLTEENLKMK  KEQ GEALHW
Sbjct: 301 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKGQKEQLGEALHW 360

Query: 380 SDYLSMPFTQNVITETLRMGNIIIGVMRKAMKDMNIKGYLIPKGWSVLTYFRSVHLDDSH 439
           SDYLSMPFTQ+VITETLRMGNIIIGVMRKAMKDM IKGYLIPKGWSVLTYFRSVHLDD+H
Sbjct: 361 SDYLSMPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNH 420

Query: 440 YHCPYKFNPWRW-------------------------------------------VAEED 480
           Y CPYKFNPWRW                                           +AEED
Sbjct: 421 YDCPYKFNPWRWQDREMSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTHFRWIAEED 474

[Close]

BLAST of Cp4.1LG12g08830 vs. ExPASy TrEMBL
Match: A0A1S3CGU6 (3-epi-6-deoxocathasterone 23-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103500734 PE=3 SV=1)

HSP 1 Score: 735 bits (1898), Expect = 2.66e-264
Identity = 384/504 (76.19%), Postives = 408/504 (80.95%), Query Frame = 0


Query: 20  MDSFWDVLVLTAIFLTIILLYRNCFSLLRSKLCSHLPLGSLGWPFIGETLEFIACAYSDR 79
           MDS WDVLV+TAI    I+LYRNCF LLRSK C+ LPLGSLGWPFIGET+EFI+CAYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 80  PEAFMDKRRRLYGKVFKSHVFGSPTIVSTDAEVSKFILQSDAKAFVPCYPKSLTELMGKS 139
           PE FMDKRRRLYGKVFKSH+FGSPTIVSTDAEVSKFILQSDAKAFVP YPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 140 SILLINGSLQKRIHGLVGAFFKSSYLKAQITKDMECYVKLAMANWSENSPVYLQDETKNI 199
           SILLINGSLQKRIHGLVGAFFKSS+LKAQITKDMECYVKL+MA+W+EN+P+YLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 200 SFQVLVKILISLNPGQRMEFVKQQFQEFIAGLMALPVNIPGSRLYQSLQASWIVKALLSS 259
           SFQVLVKILISLNPGQ MEF+K+QFQEFIAGLMALPVNIPGSRLYQSLQA          
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQA---------- 240

Query: 260 FTITIFLMELPFMLELQAKRAMVKLVKKIIQERRSCGSSPVAVPQDVMEVLLKDESDLLT 319
                             KR MVKLVKKIIQER+SCG S   VP+DVMEVLLKDE++ LT
Sbjct: 241 ------------------KRNMVKLVKKIIQERKSCGIS--TVPKDVMEVLLKDENEELT 300

Query: 320 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPEALQQLTEENLKMKRLKEQAGEALHW 379
           DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCP ALQQLTEENLKMK  KEQ GEALHW
Sbjct: 301 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKGQKEQLGEALHW 360

Query: 380 SDYLSMPFTQNVITETLRMGNIIIGVMRKAMKDMNIKGYLIPKGWSVLTYFRSVHLDDSH 439
           SDYLSMPFTQ+VITETLRMGNIIIGVMRKAMKDM IKGYLI KGWSVLTYFRSVHLDD+H
Sbjct: 361 SDYLSMPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLILKGWSVLTYFRSVHLDDNH 420

Query: 440 YHCPYKFNPWRW-------------------------------------------VAEED 480
           Y CPYKFNPWRW                                           +AEED
Sbjct: 421 YDCPYKFNPWRWQERELSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTHFRWIAEED 471

[Close]

BLAST of Cp4.1LG12g08830 vs. ExPASy TrEMBL
Match: A0A5A7UW93 (Cytochrome p450 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold242G001660 PE=3 SV=1)

HSP 1 Score: 717 bits (1851), Expect = 8.02e-254
Identity = 367/442 (83.03%), Postives = 387/442 (87.56%), Query Frame = 0


Query: 20  MDSFWDVLVLTAIFLTIILLYRNCFSLLRSKLCSHLPLGSLGWPFIGETLEFIACAYSDR 79
           MDS WDVLV+TAI    I+LYRNCF LLRSK C+ LPLGSLGWPFIGET+EFI+CAYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 80  PEAFMDKRRRLYGKVFKSHVFGSPTIVSTDAEVSKFILQSDAKAFVPCYPKSLTELMGKS 139
           PE FMDKRRRLYGKVFKSH+FGSPTIVSTDAEVSKFILQSDAKAFVP YPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 140 SILLINGSLQKRIHGLVGAFFKSSYLKAQITKDMECYVKLAMANWSENSPVYLQDETKNI 199
           SILLINGSLQKRIHGLVGAFFKSS+LKAQITKDMECYVKL+MA+W+EN+P+YLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 200 SFQVLVKILISLNPGQRMEFVKQQFQEFIAGLMALPVNIPGSRLYQSLQASWIVKALLSS 259
           SFQVLVKILISLNPGQ MEF+K+QFQEFIAGLMALPVNIPGSRLYQSLQASW        
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQASW-------- 240

Query: 260 FTITIFLMELPFMLELQAKRAMVKLVKKIIQERRSCGSSPVAVPQDVMEVLLKDESDLLT 319
                            AKR MVKLVKKIIQER+SCG S   VP+DVMEVLLKDE++ LT
Sbjct: 241 ----------------TAKRNMVKLVKKIIQERKSCGIS--TVPKDVMEVLLKDENEELT 300

Query: 320 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPEALQQLTEENLKMKRLKEQAGEALHW 379
           DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCP ALQQLTEENLKMK  KEQ GEALHW
Sbjct: 301 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKGQKEQLGEALHW 360

Query: 380 SDYLSMPFTQNVITETLRMGNIIIGVMRKAMKDMNIKGYLIPKGWSVLTYFRSVHLDDSH 439
           SDYLSMPFTQ+VITETLRMGNIIIGVMRKAMKDM IKGYLIPKGWSVLTYFRSVHLDD+H
Sbjct: 361 SDYLSMPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNH 413

Query: 440 YHCPYKFNPWRWVAEEDRIINF 461
           Y CPYKFNPWRW   E    NF
Sbjct: 421 YDCPYKFNPWRWQERELSSCNF 413

[Close]

BLAST of Cp4.1LG12g08830 vs. ExPASy TrEMBL
Match: E5GCA5 (Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 705 bits (1819), Expect = 4.38e-249
Identity = 363/442 (82.13%), Postives = 383/442 (86.65%), Query Frame = 0


Query: 20  MDSFWDVLVLTAIFLTIILLYRNCFSLLRSKLCSHLPLGSLGWPFIGETLEFIACAYSDR 79
           MDS WDVLV+TAI    I+LYRNCF LLRSK C+ LPLGSLGWPFIGET+EFI+CAYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 80  PEAFMDKRRRLYGKVFKSHVFGSPTIVSTDAEVSKFILQSDAKAFVPCYPKSLTELMGKS 139
           PE FMDKRRRLYGKVFKSH+FGSPTIVSTDAEVSKFILQSDAKAFVP YPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 140 SILLINGSLQKRIHGLVGAFFKSSYLKAQITKDMECYVKLAMANWSENSPVYLQDETKNI 199
           SILLINGSLQKRIHGLVGAFFKSS+LKAQITKDMECYVKL+MA+W+EN+P+YLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 200 SFQVLVKILISLNPGQRMEFVKQQFQEFIAGLMALPVNIPGSRLYQSLQASWIVKALLSS 259
           SFQVLVKILISLNPGQ MEF+K+QFQEFIAGLMALPVNIPGSRLYQSLQA          
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQA---------- 240

Query: 260 FTITIFLMELPFMLELQAKRAMVKLVKKIIQERRSCGSSPVAVPQDVMEVLLKDESDLLT 319
                             KR MVKLVKKIIQER+SCG S   VP+DVMEVLLKDE++ LT
Sbjct: 241 ------------------KRNMVKLVKKIIQERKSCGIS--TVPKDVMEVLLKDENEELT 300

Query: 320 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPEALQQLTEENLKMKRLKEQAGEALHW 379
           DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCP ALQQLTEENLKMK  KEQ GEALHW
Sbjct: 301 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKGQKEQLGEALHW 360

Query: 380 SDYLSMPFTQNVITETLRMGNIIIGVMRKAMKDMNIKGYLIPKGWSVLTYFRSVHLDDSH 439
           SDYLSMPFTQ+VITETLRMGNIIIGVMRKAMKDM IKGYLI KGWSVLTYFRSVHLDD+H
Sbjct: 361 SDYLSMPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLILKGWSVLTYFRSVHLDDNH 409

Query: 440 YHCPYKFNPWRWVAEEDRIINF 461
           Y CPYKFNPWRW   E    NF
Sbjct: 421 YDCPYKFNPWRWQERELSSCNF 409

[Close]

BLAST of Cp4.1LG12g08830 vs. ExPASy TrEMBL
Match: A0A6P3YVG2 (3-epi-6-deoxocathasterone 23-monooxygenase isoform X1 OS=Ziziphus jujuba OX=326968 GN=LOC107403831 PE=3 SV=1)

HSP 1 Score: 624 bits (1608), Expect = 4.11e-220
Identity = 325/506 (64.23%), Postives = 377/506 (74.51%), Query Frame = 0


Query: 20  MDSFWDVLVLTAIFLT--IILLYRNCFSLLRSKLCSHLPLGSLGWPFIGETLEFIACAYS 79
           MD  W +L +TA+FL+  I+LLY+N +          LPLG+LGWPF+GET+EF++CAYS
Sbjct: 1   MDKLW-ILQVTAVFLSAIILLLYKNSYRFRSKHRRKPLPLGTLGWPFLGETIEFVSCAYS 60

Query: 80  DRPEAFMDKRRRLYGKVFKSHVFGSPTIVSTDAEVSKFILQSDAKAFVPCYPKSLTELMG 139
           DRPE+FMDKRR +YGKVFKSH+FGSPTIVSTDAEVSKF+LQSDAKAFVP YPKSLTELMG
Sbjct: 61  DRPESFMDKRRSMYGKVFKSHIFGSPTIVSTDAEVSKFVLQSDAKAFVPSYPKSLTELMG 120

Query: 140 KSSILLINGSLQKRIHGLVGAFFKSSYLKAQITKDMECYVKLAMANWSENSPVYLQDETK 199
           KSSILLINGSLQ+RIHGLVGAFFKS +LKAQIT+DM+ YV+ +MANWSE+ P Y+QD TK
Sbjct: 121 KSSILLINGSLQRRIHGLVGAFFKSPHLKAQITRDMQKYVQESMANWSEDHPTYIQDVTK 180

Query: 200 NISFQVLVKILISLNPGQRMEFVKQQFQEFIAGLMALPVNIPGSRLYQSLQASWIVKALL 259
            I+FQVLVK LISLNPG  MEF+K+QFQEFIAGLM+LP+NIPGS LY+SLQA        
Sbjct: 181 TIAFQVLVKALISLNPGDEMEFLKKQFQEFIAGLMSLPINIPGSTLYRSLQA-------- 240

Query: 260 SSFTITIFLMELPFMLELQAKRAMVKLVKKIIQERRSCGSSPVAVPQDVMEVLLKDESDL 319
                               K+ MVKLVKK IQ R++  S     P+DV++VLL D S+ 
Sbjct: 241 --------------------KKKMVKLVKKTIQARKNT-SGISNFPKDVVDVLLSDTSEQ 300

Query: 320 LTDDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPEALQQLTEENLKMKRLKEQAGEAL 379
           LTDDLIA+NMIDMMIPGEDSVP+LMTLA+KYLSDCP ALQQL EEN+K+KR K+Q GE L
Sbjct: 301 LTDDLIADNMIDMMIPGEDSVPVLMTLAIKYLSDCPAALQQLMEENMKLKRHKDQLGEPL 360

Query: 380 HWSDYLSMPFTQNVITETLRMGNIIIGVMRKAMKDMNIKGYLIPKGWSVLTYFRSVHLDD 439
            WSDYLS+PFTQNVITETLRMGNIIIGVMRKAMKD+ IKGYLIPKGW V  YFRSVHLD+
Sbjct: 361 CWSDYLSLPFTQNVITETLRMGNIIIGVMRKAMKDVEIKGYLIPKGWCVFAYFRSVHLDE 420

Query: 440 SHYHCPYKFNPWRW-------------------------------------------VAE 480
           +HY  PY+FNPWRW                                           VAE
Sbjct: 421 NHYDWPYEFNPWRWQDKDMSTCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWVAE 476

[Close]

BLAST of Cp4.1LG12g08830 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 496.5 bits (1277), Expect = 2.4e-140
Identity = 265/518 (51.16%), Postives = 339/518 (65.44%), Query Frame = 0


Query: 20  MDSFWDVLVLTAIFLTIILLYRNCFSLLRS-----KLCSH----------LPLGSLGWPF 79
           MD+   +L  +  F  II+++     L  S     KL  H           P GSLGWP 
Sbjct: 1   MDTSSSLLFFSFFFFIIIVIFNKINGLRSSPASKKKLNDHHVTSQSHGPKFPHGSLGWPV 60

Query: 80  IGETLEFIACAYSDRPEAFMDKRRRLYGKVFKSHVFGSPTIVSTDAEVSKFILQSDAKAF 139
           IGET+EF++ AYSDRPE+FMDKRR +YG+VFKSH+FG+ TIVSTDAEV++ +LQSD+ AF
Sbjct: 61  IGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVNRAVLQSDSTAF 120

Query: 140 VPCYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSYLKAQITKDMECYVKLAMANW 199
           VP YPK++ ELMGKSSILLINGSL +R HGLVG+F KS  LKAQI +DM  ++  +M  W
Sbjct: 121 VPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDMHKFLSESMDLW 180

Query: 200 SENSPVYLQDETKNISFQVLVKILISLNPGQRMEFVKQQFQEFIAGLMALPVNIPGSRLY 259
           SE+ PV LQD +K ++F+VL K LIS+  G+ +E +K++F+ FI+GLM+LP+N PG++L+
Sbjct: 181 SEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFISGLMSLPINFPGTQLH 240

Query: 260 QSLQASWIVKALLSSFTITIFLMELPFMLELQAKRAMVKLVKKIIQ---ERRSCGSSPVA 319
           +S                            LQAK+ MVK V++II+    +         
Sbjct: 241 RS----------------------------LQAKKNMVKQVERIIEGKIRKTKNKEEDDV 300

Query: 320 VPQDVMEVLLKDESDLLTDDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPEALQQLTE 379
           + +DV++VLLKD S+ LT +LIA NMIDMMIPG DSVP+L+TLAVK+LSD P AL  LTE
Sbjct: 301 IAKDVVDVLLKDSSEHLTHNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTE 360

Query: 380 ENLKMKRLKEQAGEALHWSDYLSMPFTQNVITETLRMGNIIIGVMRKAMKDMNIKGYLIP 439
           EN+K+K LKE  GE L+W+DYLS+PFTQ VITETLRMGN+IIGVMRKAMKD+ IKGY+IP
Sbjct: 361 ENMKLKSLKELTGEPLYWNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIP 420

Query: 440 KGWSVLTYFRSVHLDDSHYHCPYKFNPW-------------------------------- 477
           KGW  L Y RSVHLD  +Y  PYKFNPW                                
Sbjct: 421 KGWCFLAYLRSVHLDKLYYESPYKFNPWRWQERDMNTSSFSPFGGGQRLCPGLDLARLET 480

[Close]

BLAST of Cp4.1LG12g08830 vs. TAIR 10
Match: AT4G36380.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 425.2 bits (1092), Expect = 6.8e-119
Identity = 218/470 (46.38%), Postives = 301/470 (64.04%), Query Frame = 0


Query: 55  LPLGSLGWPFIGETLEFIACAYSDRPEAFMDKRRRLYGKVFKSHVFGSPTIVSTDAEVSK 114
           +P GSLGWP IGETL FIAC YS RP  FMDKR+ LYGKVFK+++ G+P I+STDAEV+K
Sbjct: 69  IPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNK 128

Query: 115 FILQSDAKAFVPCYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSYLKAQITKDME 174
            +LQ+    FVP YPKS+TEL+G++SIL ING  QKR+H L+GAF +S +LK +IT+D+E
Sbjct: 129 VVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIE 188

Query: 175 CYVKLAMANWSENSPVYLQDETKNISFQVLVKILISLNPGQRMEFVKQQFQEFIAGLMAL 234
             V L +A+W++   V++QDE K ++F++LVK+L+S +PG+ M  +K +F+EFI GL+ +
Sbjct: 189 ASVVLTLASWAQLPLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFIKGLICI 248

Query: 235 PVNIPGSRLYQSLQASWIVKALLSSFTITIFLMELPFMLELQAKRAMVKLVKKIIQERRS 294
           P+  PG+RLY+S                            L+AK  ++K+VKK+++ER+ 
Sbjct: 249 PIKFPGTRLYKS----------------------------LKAKERLIKMVKKVVEERQ- 308

Query: 295 CGSSPVAVPQDVMEVLLKD----ESDLLTDDLIAENMIDMMIPGEDSVPILMTLAVKYLS 354
              +  +   DV++VLL+D    E      D ++  +++MMIPGE+++P  MTLAVK+LS
Sbjct: 309 VAMTTTSPANDVVDVLLRDGGDSEKQSQPSDFVSGKIVEMMIPGEETMPTAMTLAVKFLS 368

Query: 355 DCPEALQQLTEENLKMKRLKEQAGEALHWSDYLSMPFTQNVITETLRMGNIIIGVMRKAM 414
           D P AL +L EEN++MKR K + GE   W+DY+S+ FTQNVI ETLRM NII GV RKA+
Sbjct: 369 DNPVALAKLVEENMEMKRRKLELGEEYKWTDYMSLSFTQNVINETLRMANIINGVWRKAL 428

Query: 415 KDMNIKGYLIPKGWSVLTYFRSVHLDDSHYHCPYKFNPWR-------------------- 474
           KD+ IKGYLIPKGW VL  F SVH+D+  Y  PY+F+PWR                    
Sbjct: 429 KDVEIKGYLIPKGWCVLASFISVHMDEDIYDNPYQFDPWRWDRINGSANSSICFTPFGGG 488

[Close]

BLAST of Cp4.1LG12g08830 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 288.9 bits (738), Expect = 7.6e-78
Identity = 166/470 (35.32%), Postives = 254/470 (54.04%), Query Frame = 0


Query: 55  LPLGSLGWPFIGETLEFIACAYSDRPEAFMDKRRRLYGKVFKSHVFGSPTIVSTDAEVSK 114
           LP GSLG P IGET + I    ++ PE F+D+R   YG VF +H+FG PTI S D E ++
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 115 FILQSDAKAFVPCYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSYLKAQITKDME 174
           F+LQ++ K F   YP S+  L+GK S+LL+ GSL KR+H L  +F  SS +K  +  D++
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 175 CYVKLAMANWSENSPVYLQDETKNISFQVLVKILISLNPGQRMEFVKQQFQEFIAGLMAL 234
             V+  + +WS  S V L +E K I+F++ VK L+S +PG+  E +++++   I G  +L
Sbjct: 151 RLVRFNLDSWS--SRVLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFSL 210

Query: 235 PVNIPGSRLYQSLQASWIVKALLSSFTITIFLMELPFMLELQAKRAMVKLVKKIIQERRS 294
           P+                             L    +   +QA+R + + +  ++ +RR 
Sbjct: 211 PLP----------------------------LFSTTYRKAIQARRKVAEALTVVVMKRRE 270

Query: 295 CGSSPVAVPQDVMEVLLKDESDLLTDDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPE 354
                    +D++  LL  + D  +D+ I + ++ +++ G ++   +MTLAVK+L++ P 
Sbjct: 271 EEEEGAERKKDMLAALLAAD-DGFSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETPL 330

Query: 355 ALQQLTEENLKMKRLKEQAGEALHWSDYLSMPFTQNVITETLRMGNIIIGVMRKAMKDMN 414
           AL QL EE+ K++ +K  +  +L WSDY SMPFTQ V+ ETLR+ NII GV R+AM D+ 
Sbjct: 331 ALAQLKEEHEKIRAMKSDS-YSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVE 390

Query: 415 IKGYLIPKGWSVLTYFRSVHLDDSHYHCPYKFNPWRW----------------------- 474
           IKGY IPKGW V + FR+VHLD +H+     FNPWRW                       
Sbjct: 391 IKGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQSNSVTTGPSNVFTPFGGGPRLC 450

Query: 475 ------------------------VAEEDRIINFPTVRMKNRMPVWVKQR 478
                                    AE+D+++ FPT R + R P++VK+R
Sbjct: 451 PGYELARVALSVFLHRLVTGFSWVPAEQDKLVFFPTTRTQKRYPIFVKRR 468

[Close]

BLAST of Cp4.1LG12g08830 vs. TAIR 10
Match: AT5G05690.2 (Cytochrome P450 superfamily protein )

HSP 1 Score: 282.3 bits (721), Expect = 7.1e-76
Identity = 154/397 (38.79%), Postives = 234/397 (58.94%), Query Frame = 0


Query: 55  LPLGSLGWPFIGETLEFIACAYSDRPEAFMDKRRRLYGKVFKSHVFGSPTIVSTDAEVSK 114
           LP GSLG P IGET + I    ++ PE F+D+R   YG VF +H+FG PTI S D E ++
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 115 FILQSDAKAFVPCYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSYLKAQITKDME 174
           F+LQ++ K F   YP S+  L+GK S+LL+ GSL KR+H L  +F  SS +K  +  D++
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 175 CYVKLAMANWSENSPVYLQDETKNISFQVLVKILISLNPGQRMEFVKQQFQEFIAGLMAL 234
             V+  + +WS  S V L +E K I+F++ VK L+S +PG+  E +++++   I G  +L
Sbjct: 151 RLVRFNLDSWS--SRVLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFSL 210

Query: 235 PVNIPGSRLYQSLQASWIVKALLSSFTITIFLMELPFMLELQAKRAMVKLVKKIIQERRS 294
           P+                             L    +   +QA+R + + +  ++ +RR 
Sbjct: 211 PLP----------------------------LFSTTYRKAIQARRKVAEALTVVVMKRRE 270

Query: 295 CGSSPVAVPQDVMEVLLKDESDLLTDDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPE 354
                    +D++  LL  + D  +D+ I + ++ +++ G ++   +MTLAVK+L++ P 
Sbjct: 271 EEEEGAERKKDMLAALLAAD-DGFSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETPL 330

Query: 355 ALQQLTEENLKMKRLKEQAGEALHWSDYLSMPFTQNVITETLRMGNIIIGVMRKAMKDMN 414
           AL QL EE+ K++ +K  +  +L WSDY SMPFTQ V+ ETLR+ NII GV R+AM D+ 
Sbjct: 331 ALAQLKEEHEKIRAMKSDS-YSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVE 390

Query: 415 IKGYLIPKGWSVLTYFRSVHLDDSHYHCPYKFNPWRW 452
           IKGY IPKGW V + FR+VHLD +H+     FNPWRW
Sbjct: 391 IKGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRW 395

[Close]

BLAST of Cp4.1LG12g08830 vs. TAIR 10
Match: AT3G50660.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 251.5 bits (641), Expect = 1.3e-66
Identity = 144/429 (33.57%), Postives = 246/429 (57.34%), Query Frame = 0


Query: 29  LTAIFLTIILLYRNCFSLLRSKLCSHLPLGSLGWPFIGETLEFIACAYSDRPEAFMDKRR 88
           L ++ L +ILL R        K   +LP G  GWPF+GET+ ++    +     FM +  
Sbjct: 18  LLSLLLFLILLKRR-----NRKTRFNLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHV 77

Query: 89  RLYGKVFKSHVFGSPTIVSTDAEVSKFILQSDAKAFVPCYPKSLTELMGKSSILLINGSL 148
             YGK+++S++FG PTIVS DA +++FILQ++ + F   YP+S+  ++GK S+L++ G +
Sbjct: 78  SKYGKIYRSNLFGEPTIVSADAGLNRFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDM 137

Query: 149 QKRIHGLVGAFFKSSYLKAQITKDMECYVKLAMANWSENSPVYLQDETKNISFQVLVKIL 208
            + +  +   F   + L+  + KD+E +    + +W +NS    QDE K  +F ++ K +
Sbjct: 138 HRDMRSISLNFLSHARLRTILLKDVERHTLFVLDSWQQNSIFSAQDEAKKFTFNLMAKHI 197

Query: 209 ISLNPG-QRMEFVKQQFQEFIAGLMALPVNIPGSRLYQSLQASWIVKALLSSFTITIFLM 268
           +S++PG +  E +K+++  F+ G+++ P+N+PG+  +         KAL S  TI  F+ 
Sbjct: 198 MSMDPGEEETEQLKKEYVTFMKGVVSAPLNLPGTAYH---------KALQSRATILKFIE 257

Query: 269 ELPFMLELQAKRAMVKLVKKIIQERRSCGSSPVAVPQ----DVMEVLLKDESDLLTDDLI 328
                 +L  K    +  +   ++      S     Q    D++  +LK  +  L+ + I
Sbjct: 258 RKMEERKLDIKEEDQEEEEVKTEDEAEMSKSDHVRKQRTDDDLLGWVLKHSN--LSTEQI 317

Query: 329 AENMIDMMIPGEDSVPILMTLAVKYLSDCPEALQQLTEENLKMKRLKEQAGEA-LHWSDY 388
            + ++ ++  G ++  + + LA+ +L  CP+A+++L EE+L++ R K++ GE+ L+W DY
Sbjct: 318 LDLILSLLFAGHETSSVAIALAIFFLQACPKAVEELREEHLEIARAKKELGESELNWDDY 377

Query: 389 LSMPFTQNVITETLRMGNIIIGVMRKAMKDMNIKGYLIPKGWSVLTYFRSVHLDDSHYHC 448
             M FTQ VI ETLR+GN++  + RKA+KD+  KGY IP GW VL    +VHLD+S Y  
Sbjct: 378 KKMDFTQCVINETLRLGNVVRFLHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQ 430

Query: 449 PYKFNPWRW 452
           P  FNPWRW
Sbjct: 438 PNLFNPWRW 430

The following BLAST results are available for this feature:

BLAST of Cp4.1LG12g08830 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94IA6	3.4e-139	51.16	3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=37...	[more]
Q94IW5	1.2e-136	52.64	Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90D2 PE=1 SV...	[more]
Q9M066	9.5e-118	46.38	3-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 OS=Arabidopsis thaliana OX=37...	[more]
Q42569	1.1e-76	35.32	Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1	[more]
O64989	1.9e-65	33.57	Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2	[more]

BLAST of Cp4.1LG12g08830 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6575481.1	0.0	90.87	3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1, partial [Cucurbita argyrospe...	[more]
XP_008462361.1	5.50e-264	76.19	PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase [Cucumis melo]	[more]
KAA0059438.1	1.66e-253	83.03	cytochrome p450 [Cucumis melo var. makuwa]	[more]
XP_011659645.1	8.56e-253	82.58	LOW QUALITY PROTEIN: 3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 [Cucumis...	[more]
KAE8646515.1	1.13e-252	82.58	hypothetical protein Csa_016429 [Cucumis sativus]	[more]

BLAST of Cp4.1LG12g08830 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K7W8	2.31e-268	76.59	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G451390 PE=3 SV=1	[more]
A0A1S3CGU6	2.66e-264	76.19	3-epi-6-deoxocathasterone 23-monooxygenase OS=Cucumis melo OX=3656 GN=LOC1035007...	[more]
A0A5A7UW93	8.02e-254	83.03	Cytochrome p450 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold242G0016...	[more]
E5GCA5	4.38e-249	82.13	Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1	[more]
A0A6P3YVG2	4.11e-220	64.23	3-epi-6-deoxocathasterone 23-monooxygenase isoform X1 OS=Ziziphus jujuba OX=3269...	[more]

BLAST of Cp4.1LG12g08830 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G13730.1	2.4e-140	51.16	cytochrome P450, family 90, subfamily D, polypeptide 1	[more]
AT4G36380.1	6.8e-119	46.38	Cytochrome P450 superfamily protein	[more]
AT5G05690.1	7.6e-78	35.32	Cytochrome P450 superfamily protein	[more]
AT5G05690.2	7.1e-76	38.79	Cytochrome P450 superfamily protein	[more]
AT3G50660.1	1.3e-66	33.57	Cytochrome P450 superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 352..376
None	No IPR available	PANTHER	PTHR24286:SF30	3-EPI-6-DEOXOCATHASTERONE 23-MONOOXYGENASE CYP90D1	coord: 36..455
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 36..455
IPR002403	Cytochrome P450, E-class, group IV	PRINTS	PR00465	EP450IV	coord: 385..401 score: 35.29 coord: 434..452 score: 29.96 coord: 55..72 score: 29.7
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 48..466 e-value: 1.9E-74 score: 253.1
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 55..477
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 56..456 e-value: 8.1E-36 score: 123.7

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG12g08830.1	Cp4.1LG12g08830.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0010268	brassinosteroid homeostasis
biological_process	GO:0016125	sterol metabolic process
biological_process	GO:0016192	vesicle-mediated transport
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen