Cp4.1LG12g07960 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG12g07960 vs. NCBI nr
Match: KAG6575553.1 (Thionin-like protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 229 bits (584), Expect = 7.60e-76
Identity = 107/110 (97.27%), Postives = 109/110 (99.09%), Query Frame = 0

Query: 1   MKTVLVACLVLSFVVVGISEPTEYVGGPSCYFGCINSCVESAFQCAICIGKCMISVESSP 60
           MKTVLVACLVLSFVVVGISEPTEYVGGP+CYFGCINSC ESAFQCAICIGKCMISVES+P
Sbjct: 1   MKTVLVACLVLSFVVVGISEPTEYVGGPNCYFGCINSCFESAFQCAICIGKCMISVESTP 60

Query: 61  MQANHMDNRYFCKLGCSTSRCAKLLLNHPNEKQMKGCVNSCSHTCTNIMN 110
           MQANHMDNRYFCKLGCSTSRCAKLLLNHPNEKQMKGCVNSCSHTCTNIMN
Sbjct: 61  MQANHMDNRYFCKLGCSTSRCAKLLLNHPNEKQMKGCVNSCSHTCTNIMN 110

BLAST of Cp4.1LG12g07960 vs. NCBI nr
Match: KAG7014099.1 (hypothetical protein SDJN02_24272, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 230 bits (586), Expect = 1.63e-74
Identity = 107/110 (97.27%), Postives = 110/110 (100.00%), Query Frame = 0

Query: 1   MKTVLVACLVLSFVVVGISEPTEYVGGPSCYFGCINSCVESAFQCAICIGKCMISVESSP 60
           MKTVLVACLVLSFVVVGISEPTEYVGGP+CYFGCINSC+ESAFQCAICIGKCMISVES+P
Sbjct: 115 MKTVLVACLVLSFVVVGISEPTEYVGGPNCYFGCINSCLESAFQCAICIGKCMISVESTP 174

Query: 61  MQANHMDNRYFCKLGCSTSRCAKLLLNHPNEKQMKGCVNSCSHTCTNIMN 110
           MQANHMDNRYFCKLGCSTSRCAKLLLNHPNEKQMKGCVNSCSHTCTNIMN
Sbjct: 175 MQANHMDNRYFCKLGCSTSRCAKLLLNHPNEKQMKGCVNSCSHTCTNIMN 224

BLAST of Cp4.1LG12g07960 vs. NCBI nr
Match: KAG6575558.1 (hypothetical protein SDJN03_26197, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 203 bits (517), Expect = 2.00e-64
Identity = 91/94 (96.81%), Postives = 93/94 (98.94%), Query Frame = 0

Query: 17  GISEPTEYVGGPSCYFGCINSCVESAFQCAICIGKCMISVESSPMQANHMDNRYFCKLGC 76
           GISEPTEYVGGP+CYFGCINSC ESAFQCAICIGKCMISVES+PMQANHMDNRYFCKLGC
Sbjct: 102 GISEPTEYVGGPNCYFGCINSCFESAFQCAICIGKCMISVESTPMQANHMDNRYFCKLGC 161

Query: 77  STSRCAKLLLNHPNEKQMKGCVNSCSHTCTNIMN 110
           STSRCAKLLLNHPNEKQMKGCVNSCSHTCTNIMN
Sbjct: 162 STSRCAKLLLNHPNEKQMKGCVNSCSHTCTNIMN 195

BLAST of Cp4.1LG12g07960 vs. NCBI nr
Match: XP_008461038.1 (PREDICTED: beta-hordothionin-like [Cucumis melo])

HSP 1 Score: 109 bits (272), Expect = 4.48e-28
Identity = 59/127 (46.46%), Postives = 78/127 (61.42%), Query Frame = 0

Query: 1   MKTVLVACLVLSFVVVGISEPTEYVGGPS------------CYFGCI-NSCVESAFQCAI 60
           MK++ V CL+LS +VVG SEP   VG  S            CY  C+ N C +  FQCA+
Sbjct: 1   MKSIFVLCLILSLLVVGKSEPENDVGSGSGSGNGNDNGSSGCYISCLLNDCRKGVFQCAV 60

Query: 61  CIGKC-MISVESS-PMQANHMDNRYFCKLGCSTSRCAKLLL---NHPNEKQMKGCVNSCS 109
           C+GKC ++  ESS   ++N MDN  FCK+GC+TS C K  L    H +EK++KGCV+SCS
Sbjct: 61  CLGKCAVVGAESSYSSRSNRMDNHDFCKIGCTTSLCTKFALPNNQHSDEKEVKGCVSSCS 120

BLAST of Cp4.1LG12g07960 vs. NCBI nr
Match: KAG6575557.1 (Thionin-like protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 84.7 bits (208), Expect = 1.06e-17
Identity = 38/89 (42.70%), Postives = 51/89 (57.30%), Query Frame = 0

Query: 17  GISEPTEYVGGPSCYFGCINSCVESAFQCAICIGKCMISVESSPMQANHMDNRYFCKLGC 76
           G+ E +       CY  C  +C+    +CA+CIG CMIS + SPM  NH++N YFCKLGC
Sbjct: 10  GMPELSRNPSNGYCYALCGLACLSGPIECALCIGSCMISAQDSPMDINHLNNAYFCKLGC 69

Query: 77  STSRCAKLLLNHPNEKQMKGCVNSCSHTC 105
           +TSRC +LL N      +  C  +C   C
Sbjct: 70  ATSRCTRLLSNARTGITLGTCGATCLAKC 98

BLAST of Cp4.1LG12g07960 vs. ExPASy TrEMBL
Match: A0A1S3CDB8 (beta-hordothionin-like OS=Cucumis melo OX=3656 GN=LOC103499744 PE=4 SV=1)

HSP 1 Score: 109 bits (272), Expect = 2.17e-28
Identity = 59/127 (46.46%), Postives = 78/127 (61.42%), Query Frame = 0

Query: 1   MKTVLVACLVLSFVVVGISEPTEYVGGPS------------CYFGCI-NSCVESAFQCAI 60
           MK++ V CL+LS +VVG SEP   VG  S            CY  C+ N C +  FQCA+
Sbjct: 1   MKSIFVLCLILSLLVVGKSEPENDVGSGSGSGNGNDNGSSGCYISCLLNDCRKGVFQCAV 60

Query: 61  CIGKC-MISVESS-PMQANHMDNRYFCKLGCSTSRCAKLLL---NHPNEKQMKGCVNSCS 109
           C+GKC ++  ESS   ++N MDN  FCK+GC+TS C K  L    H +EK++KGCV+SCS
Sbjct: 61  CLGKCAVVGAESSYSSRSNRMDNHDFCKIGCTTSLCTKFALPNNQHSDEKEVKGCVSSCS 120

BLAST of Cp4.1LG12g07960 vs. ExPASy TrEMBL
Match: A0A6P5X8X4 (thionin-like protein 2 OS=Durio zibethinus OX=66656 GN=LOC111281385 PE=4 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 6.44e-17
Identity = 50/111 (45.05%), Postives = 67/111 (60.36%), Query Frame = 0

Query: 1   MKTVLVACLVLSFVVVGISEPTEYVGGPSCYFGCINSCVE----SAFQCAI-CIGKCMIS 60
           M +VL+ CLVL  +   I + T     PSCY GC   CV     SAF CA+ C+ KC++ 
Sbjct: 6   MGSVLMVCLVLGLL---IGQSTA--SFPSCYKGCFVWCVNNLGNSAFSCALKCLKKCVL- 65

Query: 61  VESSPMQANHMDNRYFCKLGCSTSRCAKLLLNH-PNEKQMKGCVNSCSHTC 105
            ++SP+  N  D +YFCKLGCSTS C  L     P+E +++ CV+SCS TC
Sbjct: 66  -QTSPV-GNIQDTQYFCKLGCSTSLCTSLSSKQDPSEGKVESCVDSCSETC 108

BLAST of Cp4.1LG12g07960 vs. ExPASy TrEMBL
Match: A0A6P8CEJ2 (thionin-like protein 2 OS=Punica granatum OX=22663 GN=LOC116196697 PE=4 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 2.31e-16
Identity = 42/87 (48.28%), Postives = 52/87 (59.77%), Query Frame = 0

Query: 28  PSCYFGCINSCV----ESAFQCAI-CIGKCMI-SVESSPMQANHMDNRYFCKLGCSTSRC 87
           PSCY  C   CV    E+AF+CA  C+  C++ S   SP  A  +D  YFCKLGCSTS C
Sbjct: 34  PSCYKSCFVLCVIKPGETAFKCAFQCLKDCLVHSASPSPASALSVDTDYFCKLGCSTSLC 93

Query: 88  AKLLLN-HPNEKQMKGCVNSCSHTCTN 107
             L    +P EK++ GCV SCS  C+N
Sbjct: 94  TNLSTKTNPGEKEVGGCVESCSGMCSN 120

BLAST of Cp4.1LG12g07960 vs. ExPASy TrEMBL
Match: A0A6J1KDK0 (thionin-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111494667 PE=4 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.13e-15
Identity = 45/110 (40.91%), Postives = 58/110 (52.73%), Query Frame = 0

Query: 1   MKTVLVACLVLSFVVVGISEPTEYVGGPSCYFGCINSC-VESAFQCAICIGKCM---ISV 60
           MK+V+V C +LS V       T   G   CY  C   C +        C GKC+   + +
Sbjct: 36  MKSVVVICFILSLVA---GRSTASFG--KCYAKCFIVCAITPGVPIGTCGGKCLADCLFL 95

Query: 61  ESSPMQANHMDNRYFCKLGCSTSRCAKLLLN-HPNEKQMKGCVNSCSHTC 105
            S+P   NH+D  YFCKLGC+TS C K      P EK+++ CVNSCS TC
Sbjct: 96  ASAPRDLNHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTC 140

BLAST of Cp4.1LG12g07960 vs. ExPASy TrEMBL
Match: A0A6P5X8W9 (thionin-like protein 2 OS=Durio zibethinus OX=66656 GN=LOC111281382 PE=4 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.50e-13
Identity = 44/111 (39.64%), Postives = 61/111 (54.95%), Query Frame = 0

Query: 1   MKTVLVACLVLSFVVVGISEPTEYVGGPSCYFGCINSCV----ESAFQCAI-CIGKCMIS 60
           M+++L+ CLV   +V    + T +    SCY GC   C+     SA  C++ C+ KC   
Sbjct: 6   MRSILMVCLVFGLLV---GQSTAFF--QSCYKGCFVLCIITPRNSALSCSVKCLKKC--- 65

Query: 61  VESSPMQANHMDNRYFCKLGCSTSRCAKLLLNH-PNEKQMKGCVNSCSHTC 105
             +SP   N  D +YFCKLGCSTS C  L     P E +++ CV+SCS TC
Sbjct: 66  --TSPF-GNLQDTQYFCKLGCSTSMCTSLSSKQDPGEGKVESCVDSCSKTC 105

BLAST of Cp4.1LG12g07960 vs. TAIR 10
Match: AT1G12672.2 (unknown protein; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G12663.1). )

HSP 1 Score: 53.5 bits (127), Expect = 1.2e-07
Identity = 28/69 (40.58%), Postives = 39/69 (56.52%), Query Frame = 0

Query: 41  SAFQCAICIGKCMISVESSPMQANHMD-NRYFCKLGCSTSRCAKL-LLNHPNEKQMKGCV 100
           S F+   CI  C+    S     N ++ N Y+CKLGCST  CA L  + +PN  ++  CV
Sbjct: 34  SPFKTFSCIKTCLEPPSSQMDSTNEINHNDYYCKLGCSTHHCASLSSIQNPNVDKVVDCV 93

Query: 101 NSCSHTCTN 108
           +SCS  C+N
Sbjct: 94  DSCSDKCSN 102

BLAST of Cp4.1LG12g07960 vs. TAIR 10
Match: AT1G12672.1 (unknown protein; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G12663.1); Has 4 Blast hits to 4 proteins in 1 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 4; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 50.4 bits (119), Expect = 1.0e-06
Identity = 24/56 (42.86%), Postives = 35/56 (62.50%), Query Frame = 0

Query: 53  MISVESSPMQANHMDNRYFCKLGCSTSRCAKL-LLNHPNEKQMKGCVNSCSHTCTN 108
           +++   S  + NH  N Y+CKLGCST  CA L  + +PN  ++  CV+SCS  C+N
Sbjct: 11  LVTQMDSTNEINH--NDYYCKLGCSTHHCASLSSIQNPNVDKVVDCVDSCSDKCSN 64