Cp4.1LG12g07350 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG12g07350
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionGlycerol kinase
LocationCp4.1LG12: 6927150 .. 6929773 (-)
RNA-Seq ExpressionCp4.1LG12g07350
SyntenyCp4.1LG12g07350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAATTGACAACTTAATAGATGAGGTTGTTAGGAAAGAAAAGAAAAGGGAATAAATAAATAAACAAAATCAACGAAATTACTCTATTTTTCCTTTTCGGTATTATCTTATCGAAATCATCAATTTCCATTTCAAAAATAAAAACGCGAAGAATAAGAAGTAGAAAGTGGTGATTTCATCGGTGGTTCATCATCTCTGTTTTCGATTTCTATTCGGATTGAAGATTTTGGGTTGAAATTCCGAAGTGAAAGAGGAGAATCCAAGAAATGGCGAAGGCGGAGGATGTTTTCGTTGGGTCAATCGATCAAGGAACCACCAGCACCAGATTTATCATTTACGATCGCTGTTCTCGACCGATCGGATCTCACCAGGTCGAATTCACTCAGTTCTACCCTGAAGCGGGGTGAGTCGCTGGATTCTCATTAATCCTTCTACCTTCCTTCCATTTTTTCTCTGTTATTACTTTCGAGAGTTTGATTTAATTTTTGTCTCCGTAGATGGGTGGAGCATGATCCTATGGAAATTCTGGAGAGCGTGAGGGTTTGTATGGCCAAAGCCGTCGATAAGGCCACCGCGGCGGGTTACAATGTGGACAATGGACTGAAGGCGATTGGGTTGACCAATCAGAGAGAAACAACTCTGGTTTGGAGCAAATCTACTGGCTCTCCCCTCCACAACGCAATCGTTTGGATGGACGCTCGTACCAGTGCTATTTGCAGGTATCCTGTGTTACTGGTTCCTGTAATTGCTTTGAAATTCTCTCGAACTGTCGGGGATTGATTCTTAATTCACTGCATTTTTTAGATTCTGTGTTCTTGGTTAATACTGTTTGCAGCAACATCGTTTGATTTATATGATTAAGTTTCGTTGTTCTGAGTGGGATTTTGTTTTTTTTTTTTTCTTGAAATACATCTAAGGGTTTGAGAGGATGTATTTTAAATTCCTTTTGTTTTGCTATGGTAGAAAATTGGAGAACGAATTATCTGGTGGGAGAACTCATTTTGTGGGTACTTGTGGTCTGCCAATTAGTACCTATTTTAGTGCAGTGAAGCTGCTTTGGCTGATGGAAAATGTGAAGGATGTTAATGAAAGTATAAAGAAGGGAGATGCTTTGTTTGGAACTATAGATACTTGGTTGATATGGAACTTAACTGGTGGTGTCAATGGAGGATTACATGTGACCGATGTTTCTAATGCGTCTCGAACGATGTTGATGAACCTCAAGACCCTTGAATGGGACAAACCTACATTAGAAACACTAGGAATTCCAGCACAAATCTTGCCTAAGATTGTTAGCAACTCAGAGGTCATAGGGAAAATTGTCCAGGGTTGGCCGATTCCTGGCGTTCCGATTTCGGGATGTTTAGGCGACCAACATGCTGCGATGCTTGGTCAAGCTTGTAGGAAAGGTGAGGCTAAAAGTACATATGGGACAGGTGCTTTTATACTCCTCAACACAGGGGAAGAAGTTGTTCAGTCTAAGCATGGCCTTCTAACCACTTTGGCATTCAAGCTTGGCAAGGATGCTCGAGCAAATTATGCTCTTGAAGGCTCCATTGCCATTGCTGGGGCTGCAGTTCAGTGGCTTAGAGATAGTCTTGGAATTATTAGCAGTGCGAAAGAGATCGAGGAATTGGCATCACAGGTTAAGTCCACTGGTGGGATATATTTTGTACCTGCCTTTAATGGGTTGTTTGCTCCATGGTGGCGTGACGATGCTCGTGGAGTCTGTATTGGGATCACAAGGTTTACCAGCAAGGCTCATATTGCTCGTGCCGTGCTTGAGAGTATGTGTTTTCAAGTGAAGGACGTGTTGGATTCAATGCACAAAGATGCTGGAGAGAAGGGCGAGGTGAAGAACAAGAAAGGGGAATTCTTGCTTCGAGTCGATGGTGGTGCTACTGTCAACAATCTTTTGATGCAAATTCAGGTTTCATACTCCTATTCCACACTGTACACGTTTTGTCACTCTTAACAATTTGATATGGTTCTTCTTTAACCCTGCTGTTGTGATCATTTCTGTAGGCTGACCTGTTGGGAAGTCCAGTGGTGCGACCAGCCGACATCGAGACAACTGCCCTCGGAGCAGCTTATGCAGCAGGACTAGCAGTTGGAGTTTGGACCGAAGATGAGATTTTTAGTGCAGGAGAAAGGGTTGAGAGTGCAACCACCTTCCATCCTGTATTAGAAGAAGAGTTGAGGAAGAAGAAGGTGGAATCATGGTGCAAAGCTGTTTCAAGAACCTTTGACTTGGCCGATCTTTCTCTTTGATGGTCCTTCCTTCAACTTTATAACATATTTCCTTCTTTACTTATCCTGTTCTTGTGCATTTGCTTCTTTACTACCTGTTCATGAATTACATTCTCCCACAATAAATTAAAATACAGTGGAATAACACGACGGTGGAACCAACTTTGATTACAATTGGATTCAAAAGGTCTCACTTGTGATTATTGTGCAGTCTGATTGGTTTGAAGGCTTCAAGGTGATCATGTATTTAAATCATTAGCTTTAGATGCATCTTATTGTCGCGTCTTTAGGATAGGAAAACAAGTGGCTATTCAATTTGTTGTTATTTGATAGATCCAGACAACATAGCTTCTATGTAATGGCGTCAGCCTACCG

mRNA sequence

CAAATTGACAACTTAATAGATGAGGTTGTTAGGAAAGAAAAGAAAAGGGAATAAATAAATAAACAAAATCAACGAAATTACTCTATTTTTCCTTTTCGGTATTATCTTATCGAAATCATCAATTTCCATTTCAAAAATAAAAACGCGAAGAATAAGAAGTAGAAAGTGGTGATTTCATCGGTGGTTCATCATCTCTGTTTTCGATTTCTATTCGGATTGAAGATTTTGGGTTGAAATTCCGAAGTGAAAGAGGAGAATCCAAGAAATGGCGAAGGCGGAGGATGTTTTCGTTGGGTCAATCGATCAAGGAACCACCAGCACCAGATTTATCATTTACGATCGCTGTTCTCGACCGATCGGATCTCACCAGGTCGAATTCACTCAGTTCTACCCTGAAGCGGGAAAATTGGAGAACGAATTATCTGGTGGGAGAACTCATTTTGTGGGTACTTGTGGTCTGCCAATTAGTACCTATTTTAGTGCAGTGAAGCTGCTTTGGCTGATGGAAAATGTGAAGGATGTTAATGAAAGTATAAAGAAGGGAGATGCTTTGTTTGGAACTATAGATACTTGGTTGATATGGAACTTAACTGGTGGTGTCAATGGAGGATTACATGTGACCGATGTTTCTAATGCGTCTCGAACGATGTTGATGAACCTCAAGACCCTTGAATGGGACAAACCTACATTAGAAACACTAGGAATTCCAGCACAAATCTTGCCTAAGATTGTTAGCAACTCAGAGGTCATAGGGAAAATTGTCCAGGGTTGGCCGATTCCTGGCGTTCCGATTTCGGGATGTTTAGGCGACCAACATGCTGCGATGCTTGGTCAAGCTTGTAGGAAAGGTGAGGCTAAAAGTACATATGGGACAGGTGCTTTTATACTCCTCAACACAGGGGAAGAAGTTGTTCAGTCTAAGCATGGCCTTCTAACCACTTTGGCATTCAAGCTTGGCAAGGATGCTCGAGCAAATTATGCTCTTGAAGGCTCCATTGCCATTGCTGGGGCTGCAGTTCAGTGGCTTAGAGATAGTCTTGGAATTATTAGCAGTGCGAAAGAGATCGAGGAATTGGCATCACAGGTTAAGTCCACTGGTGGGATATATTTTGTACCTGCCTTTAATGGGTTGTTTGCTCCATGGTGGCGTGACGATGCTCGTGGAGTCTGTATTGGGATCACAAGGTTTACCAGCAAGGCTCATATTGCTCGTGCCGTGCTTGAGAGTATGTGTTTTCAAGTGAAGGACGTGTTGGATTCAATGCACAAAGATGCTGGAGAGAAGGGCGAGGTGAAGAACAAGAAAGGGGAATTCTTGCTTCGAGTCGATGGTGGTGCTACTGTCAACAATCTTTTGATGCAAATTCAGGCTGACCTGTTGGGAAGTCCAGTGGTGCGACCAGCCGACATCGAGACAACTGCCCTCGGAGCAGCTTATGCAGCAGGACTAGCAGTTGGAGTTTGGACCGAAGATGAGATTTTTAGTGCAGGAGAAAGGGTTGAGAGTGCAACCACCTTCCATCCTGTATTAGAAGAAGAGTTGAGGAAGAAGAAGGTGGAATCATGGTGCAAAGCTGTTTCAAGAACCTTTGACTTGGCCGATCTTTCTCTTTGATGGTCCTTCCTTCAACTTTATAACATATTTCCTTCTTTACTTATCCTGTTCTTGTGCATTTGCTTCTTTACTACCTGTTCATGAATTACATTCTCCCACAATAAATTAAAATACAGTGGAATAACACGACGGTGGAACCAACTTTGATTACAATTGGATTCAAAAGGTCTCACTTGTGATTATTGTGCAGTCTGATTGGTTTGAAGGCTTCAAGGTGATCATGTATTTAAATCATTAGCTTTAGATGCATCTTATTGTCGCGTCTTTAGGATAGGAAAACAAGTGGCTATTCAATTTGTTGTTATTTGATAGATCCAGACAACATAGCTTCTATGTAATGGCGTCAGCCTACCG

Coding sequence (CDS)

ATGGCGAAGGCGGAGGATGTTTTCGTTGGGTCAATCGATCAAGGAACCACCAGCACCAGATTTATCATTTACGATCGCTGTTCTCGACCGATCGGATCTCACCAGGTCGAATTCACTCAGTTCTACCCTGAAGCGGGAAAATTGGAGAACGAATTATCTGGTGGGAGAACTCATTTTGTGGGTACTTGTGGTCTGCCAATTAGTACCTATTTTAGTGCAGTGAAGCTGCTTTGGCTGATGGAAAATGTGAAGGATGTTAATGAAAGTATAAAGAAGGGAGATGCTTTGTTTGGAACTATAGATACTTGGTTGATATGGAACTTAACTGGTGGTGTCAATGGAGGATTACATGTGACCGATGTTTCTAATGCGTCTCGAACGATGTTGATGAACCTCAAGACCCTTGAATGGGACAAACCTACATTAGAAACACTAGGAATTCCAGCACAAATCTTGCCTAAGATTGTTAGCAACTCAGAGGTCATAGGGAAAATTGTCCAGGGTTGGCCGATTCCTGGCGTTCCGATTTCGGGATGTTTAGGCGACCAACATGCTGCGATGCTTGGTCAAGCTTGTAGGAAAGGTGAGGCTAAAAGTACATATGGGACAGGTGCTTTTATACTCCTCAACACAGGGGAAGAAGTTGTTCAGTCTAAGCATGGCCTTCTAACCACTTTGGCATTCAAGCTTGGCAAGGATGCTCGAGCAAATTATGCTCTTGAAGGCTCCATTGCCATTGCTGGGGCTGCAGTTCAGTGGCTTAGAGATAGTCTTGGAATTATTAGCAGTGCGAAAGAGATCGAGGAATTGGCATCACAGGTTAAGTCCACTGGTGGGATATATTTTGTACCTGCCTTTAATGGGTTGTTTGCTCCATGGTGGCGTGACGATGCTCGTGGAGTCTGTATTGGGATCACAAGGTTTACCAGCAAGGCTCATATTGCTCGTGCCGTGCTTGAGAGTATGTGTTTTCAAGTGAAGGACGTGTTGGATTCAATGCACAAAGATGCTGGAGAGAAGGGCGAGGTGAAGAACAAGAAAGGGGAATTCTTGCTTCGAGTCGATGGTGGTGCTACTGTCAACAATCTTTTGATGCAAATTCAGGCTGACCTGTTGGGAAGTCCAGTGGTGCGACCAGCCGACATCGAGACAACTGCCCTCGGAGCAGCTTATGCAGCAGGACTAGCAGTTGGAGTTTGGACCGAAGATGAGATTTTTAGTGCAGGAGAAAGGGTTGAGAGTGCAACCACCTTCCATCCTGTATTAGAAGAAGAGTTGAGGAAGAAGAAGGTGGAATCATGGTGCAAAGCTGTTTCAAGAACCTTTGACTTGGCCGATCTTTCTCTTTGA

Protein sequence

MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAGKLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIWNLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIVQGWPIPGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTLAFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAFNGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNKKGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIFSAGERVESATTFHPVLEEELRKKKVESWCKAVSRTFDLADLSL
Homology
BLAST of Cp4.1LG12g07350 vs. ExPASy Swiss-Prot
Match: Q9M8L4 (Glycerol kinase OS=Arabidopsis thaliana OX=3702 GN=GLPK PE=1 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 1.4e-208
Identity = 366/523 (69.98%), Postives = 409/523 (78.20%), Query Frame = 0

Query: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAG-------------- 60
           MAK E+ F+GSIDQGTTSTRFIIYD  +RP+ SHQVEFTQFYPEAG              
Sbjct: 1   MAK-ENGFIGSIDQGTTSTRFIIYDHDARPVASHQVEFTQFYPEAGWVEHDPMEILESVK 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  VCIAKALDKATADGHNVDGGLKAIGLTDQRETTVVWSKSTGLPLHKAIVWMDARTSSICR 120

Query: 121 KLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIW 180
           +LE ELSGGR+HFV +CGLPISTYFSA+KLLWLMENV DV ++IKKGDA+FGTIDTWLIW
Sbjct: 121 RLEKELSGGRSHFVESCGLPISTYFSAMKLLWLMENVDDVKDAIKKGDAIFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV 240
           N+TGG+NGGLHVTDV+NASRTMLMNLKTL WD+ TL+TLGIPA+ILPKIVSNSEVIG+I 
Sbjct: 181 NMTGGINGGLHVTDVTNASRTMLMNLKTLSWDQDTLKTLGIPAEILPKIVSNSEVIGEIC 240

Query: 241 QGWPIPGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300
           +GWPIPG+ I+GCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGE  ++S HGLLTTL
Sbjct: 241 KGWPIPGIKIAGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEVPIKSGHGLLTTL 300

Query: 301 AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF 360
           A+KLG  A+ NYALEGSIAIAGAAVQWLRDSLGII SA EIE+LA+ V STGG+YFVPAF
Sbjct: 301 AYKLGPQAQTNYALEGSIAIAGAAVQWLRDSLGIIKSASEIEDLAAMVDSTGGVYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNK 420
           NGLFAPWWR+DARGVCIGITRFT+K+HIARAVLESMCFQVKDVLDSM+KDAGEKG + N 
Sbjct: 361 NGLFAPWWREDARGVCIGITRFTNKSHIARAVLESMCFQVKDVLDSMNKDAGEKGSLNNG 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIF 450
           KGEFLLRVDGGAT NNLLMQIQADL+GSPVVRP DIETTALGAAYAAGLAVG W E +IF
Sbjct: 421 KGEFLLRVDGGATANNLLMQIQADLMGSPVVRPVDIETTALGAAYAAGLAVGFWKEADIF 480

BLAST of Cp4.1LG12g07350 vs. ExPASy Swiss-Prot
Match: B8GC51 (Glycerol kinase OS=Chloroflexus aggregans (strain MD-66 / DSM 9485) OX=326427 GN=glpK PE=3 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 4.6e-122
Identity = 242/505 (47.92%), Postives = 312/505 (61.78%), Query Frame = 0

Query: 8   FVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAGKLE------------------ 67
           +  +IDQGTTSTR +I+D     I   Q E  Q YP  G +E                  
Sbjct: 4   YAAAIDQGTTSTRCMIFDHSGNVICYDQKEHEQIYPRPGWVEHSPDEIWERTQSVIRGAL 63

Query: 68  ------------------------------------------------NELS--GGRTHF 127
                                                           NEL+  GG+  F
Sbjct: 64  SKGGLSASDIVAVGITNQRETTVVWNRKTGRPVYNAIVWQDTRTDQICNELAADGGQDRF 123

Query: 128 VGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIWNLTGGVNGGLHVT 187
               GLP++TYFS  K+ W+++NV D  E+ + GD +FG IDT+L W LTGG NGG+HVT
Sbjct: 124 RAKVGLPLATYFSGPKIRWILDNVPDAREAAEAGDVVFGNIDTFLTWWLTGGPNGGVHVT 183

Query: 188 DVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIVQGWPIPGVPISGC 247
           DV+NASRTMLMNL+TL+WD   L  +GIP Q+LPKIV +S V G  V    + GVP++G 
Sbjct: 184 DVTNASRTMLMNLETLDWDDEILSVMGIPRQMLPKIVPSSMVYGTAV--GELAGVPVAGI 243

Query: 248 LGDQHAAMLGQAC-RKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTLAFKLGKDARANY 307
           LGDQ AAM+GQ C   GEAK+TYGTG+F+LLNTG ++V SK GLLTT+ +K G D  A Y
Sbjct: 244 LGDQQAAMVGQTCFDVGEAKNTYGTGSFMLLNTGTKIVPSKSGLLTTVCYKFG-DQPAVY 303

Query: 308 ALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAFNGLFAPWWRDDA 367
           ALEGSIAI GA VQWLRD+LG+I+++ E+E LA+ V+  GGIYFVPAF+GLFAP+WR DA
Sbjct: 304 ALEGSIAITGALVQWLRDNLGLITTSAEVEALANLVEDNGGIYFVPAFSGLFAPYWRSDA 363

Query: 368 RGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNKKGEFLLRVDGGA 427
           RGV +G+TR+ +K H+ARAVLE+  +Q ++VLD+M +D+G K           L+VDGG 
Sbjct: 364 RGVIVGLTRYVNKGHLARAVLEATAYQTREVLDAMEQDSGVKLTA--------LKVDGGM 423

Query: 428 TVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIFSAGERVESATTF 444
             NN LMQ QAD+LG PV+RP   ETT+LGAAYAAGLAVG W+  +   A   V+   T+
Sbjct: 424 VYNNTLMQFQADILGVPVIRPKVAETTSLGAAYAAGLAVGFWSNTDEMRANWGVDH--TW 483

BLAST of Cp4.1LG12g07350 vs. ExPASy Swiss-Prot
Match: B9LD34 (Glycerol kinase OS=Chloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl) OX=480224 GN=glpK PE=3 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 2.5e-120
Identity = 241/505 (47.72%), Postives = 310/505 (61.39%), Query Frame = 0

Query: 8   FVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAGKLE------------------ 67
           +  +IDQGTTSTR +I+D     I   Q E  Q YP  G +E                  
Sbjct: 4   YAAAIDQGTTSTRCMIFDHSGNVICYDQKEHEQIYPRPGWVEHSPDEIWERTQSVIRGAL 63

Query: 68  ------------------------------------------------NELS--GGRTHF 127
                                                           NEL+  GG+  F
Sbjct: 64  SKGGLSASDIVAVGITNQRETTVVWNRKTGRPVYNAIVWQDTRTDQICNELAADGGQDRF 123

Query: 128 VGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIWNLTGGVNGGLHVT 187
               GLP++TYFS  K+ W+++NV    E+ + GD +FG IDT+L W LTGG NGG+HVT
Sbjct: 124 RPKVGLPLATYFSGPKIRWILDNVPGAREAAEAGDVVFGNIDTFLTWWLTGGPNGGVHVT 183

Query: 188 DVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIVQGWPIPGVPISGC 247
           DV+NASRTMLMNL+TL+WD   L  +GIP Q+LPKIV +S V G       + GVP++G 
Sbjct: 184 DVTNASRTMLMNLETLDWDDEILGIMGIPRQMLPKIVPSSMVYGTAT--GELAGVPVAGI 243

Query: 248 LGDQHAAMLGQAC-RKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTLAFKLGKDARANY 307
           LGDQ AAM+GQ C   GEAK+TYGTG+F+LLNTG ++V SK GLLTT+ +K G D  A Y
Sbjct: 244 LGDQQAAMVGQTCFDVGEAKNTYGTGSFMLLNTGTKLVPSKSGLLTTVCYKFG-DQPAVY 303

Query: 308 ALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAFNGLFAPWWRDDA 367
           ALEGSIAI GA VQWLRD+LG+I+S+ E+E LA+ V+  GGIYFVPAF+GLFAP+WR DA
Sbjct: 304 ALEGSIAITGALVQWLRDNLGLITSSAEVEALANLVEDNGGIYFVPAFSGLFAPYWRSDA 363

Query: 368 RGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNKKGEFLLRVDGGA 427
           RGV +G+TR+ +K H+ARAVLE+  +Q ++VLD+M +D+G K           L+VDGG 
Sbjct: 364 RGVIVGLTRYVNKGHLARAVLEATAYQTREVLDAMEQDSGVKLTA--------LKVDGGM 423

Query: 428 TVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIFSAGERVESATTF 444
             NN LMQ QAD+LG PV+RP   ETT+LGAAYAAGLAVG W+  +   A   V+   T+
Sbjct: 424 VYNNTLMQFQADILGVPVIRPKVAETTSLGAAYAAGLAVGFWSNTDEMRANWGVDH--TW 483

BLAST of Cp4.1LG12g07350 vs. ExPASy Swiss-Prot
Match: A9WJ21 (Glycerol kinase OS=Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl) OX=324602 GN=glpK PE=3 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 4.3e-120
Identity = 241/505 (47.72%), Postives = 310/505 (61.39%), Query Frame = 0

Query: 8   FVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAGKLE------------------ 67
           +  +IDQGTTSTR +I+D     I   Q E  Q YP  G +E                  
Sbjct: 4   YAAAIDQGTTSTRCMIFDHSGNVICYDQKEHEQIYPRPGWVEHSPDEIWERTQSVIRGAL 63

Query: 68  ------------------------------------------------NELS--GGRTHF 127
                                                           NEL+  GG+  F
Sbjct: 64  SKGGLSASDIVAVGITNQRETTVVWNRKTGRPVYNAIVWQDTRTDQICNELAADGGQDRF 123

Query: 128 VGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIWNLTGGVNGGLHVT 187
               GLP++TYFS  K+ W+++NV    E+ + GD +FG IDT+L W LTGG NGG+HVT
Sbjct: 124 RPKVGLPLATYFSGPKIRWILDNVPGAREAAEAGDVVFGNIDTFLTWWLTGGPNGGVHVT 183

Query: 188 DVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIVQGWPIPGVPISGC 247
           DV+NASRTMLMNL+TL+WD   L  +GIP Q+LPKIV +S V G       + GVP++G 
Sbjct: 184 DVTNASRTMLMNLETLDWDDEILGIMGIPRQMLPKIVPSSMVYGTAT--GELAGVPVAGI 243

Query: 248 LGDQHAAMLGQAC-RKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTLAFKLGKDARANY 307
           LGDQ AAM+GQ C   GEAK+TYGTG+F+LLNTG ++V SK GLLTT+ +K G D  A Y
Sbjct: 244 LGDQQAAMVGQTCFDVGEAKNTYGTGSFMLLNTGTKLVPSKSGLLTTVCYKFG-DQPAVY 303

Query: 308 ALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAFNGLFAPWWRDDA 367
           ALEGSIAI GA VQWLRD+LG+I+S+ E+E LA+ V+  GGIYFVPAF+GLFAP+WR DA
Sbjct: 304 ALEGSIAITGALVQWLRDNLGLITSSAEVEALANLVEDNGGIYFVPAFSGLFAPYWRSDA 363

Query: 368 RGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNKKGEFLLRVDGGA 427
           RGV +G+TR+ +K H+ARAVLE+  +Q ++VLD+M +D+G K           L+VDGG 
Sbjct: 364 RGVIVGLTRYVNKDHLARAVLEATAYQTREVLDAMEQDSGVKLTA--------LKVDGGM 423

Query: 428 TVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIFSAGERVESATTF 444
             NN LMQ QAD+LG PV+RP   ETT+LGAAYAAGLAVG W+  +   A   V+   T+
Sbjct: 424 VYNNTLMQFQADILGVPVIRPKVAETTSLGAAYAAGLAVGFWSNTDEMRANWGVDH--TW 483

BLAST of Cp4.1LG12g07350 vs. ExPASy Swiss-Prot
Match: A6WXV2 (Glycerol kinase OS=Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / JCM 21032 / NBRC 15819 / NCTC 12168) OX=439375 GN=glpK PE=3 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 1.2e-119
Identity = 242/505 (47.92%), Postives = 307/505 (60.79%), Query Frame = 0

Query: 8   FVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAGKLENE---------------- 67
           F+GSIDQGTTS+RFII+DR    + S Q E  Q YP+AG +E+                 
Sbjct: 4   FIGSIDQGTTSSRFIIFDRQGDIVASDQREHEQIYPKAGWVEHNPIEIWRNTQHVIAAAL 63

Query: 68  ----------------------------------------------------LSGGRTHF 127
                                                                 GG    
Sbjct: 64  KKAKLKASDIASVGITNQRETTLLWDRKTGAPLYNAIVWMDTRTDELVSRYTKDGGADQL 123

Query: 128 VGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIWNLTGGVNGGLHVT 187
               GLPISTYFS +KL W+++NV    E  + GDALFGTIDTWL+WNLTGG  GG+H+T
Sbjct: 124 RAKTGLPISTYFSGLKLRWILDNVPGAREKAEAGDALFGTIDTWLVWNLTGGTEGGIHIT 183

Query: 188 DVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIVQGWPIPGVPISGC 247
           DV+NASRT LM+L TL+WD+  L    IP+  LP+I S+SEV G+I     + GV ++G 
Sbjct: 184 DVTNASRTQLMDLSTLQWDEDILRLFDIPSACLPEIRSSSEVYGEITLP-SLSGVKLAGI 243

Query: 248 LGDQHAAMLGQAC-RKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTLAFKLGKDARANY 307
           LGDQ AA+ GQAC   GEAK+TYGTG F+L+NTGE++V S +GLLTT+A+KL   A+  Y
Sbjct: 244 LGDQQAALFGQACLEPGEAKNTYGTGCFMLMNTGEKLVPSNYGLLTTVAYKL-DGAKPVY 303

Query: 308 ALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAFNGLFAPWWRDDA 367
           ALEGSIAI GA VQWLRD+LGII ++ +IE LA  V+  G +YFVPAF+GLFAP W+D A
Sbjct: 304 ALEGSIAITGALVQWLRDNLGIIRNSSDIETLARTVEDNGDVYFVPAFSGLFAPHWQDSA 363

Query: 368 RGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNKKGEFLLRVDGGA 427
           RG+  G+TRF +K HIARA LE+  +QV++VLD+M KD+G   E+        LR DGG 
Sbjct: 364 RGIIAGLTRFANKGHIARAALEASAYQVREVLDAMVKDSGV--EITE------LRADGGM 423

Query: 428 TVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVW--TEDEIFSAGERVESAT 442
           T+N LLMQ Q+D+L  PVVRP  IETTALGAAYAAGLAVG W  T+D +    E  +   
Sbjct: 424 TINELLMQFQSDILDVPVVRPKIIETTALGAAYAAGLAVGYWKSTKDIV----ENWQVGH 483

BLAST of Cp4.1LG12g07350 vs. NCBI nr
Match: XP_023548708.1 (glycerol kinase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 867 bits (2239), Expect = 1.47e-315
Identity = 449/523 (85.85%), Postives = 449/523 (85.85%), Query Frame = 0

Query: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAG-------------- 60
           MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAG              
Sbjct: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAGWVEHDPMEILESVR 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  VCMAKAVDKATAAGYNVDNGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDARTSAICR 120

Query: 121 KLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIW 180
           KLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIW
Sbjct: 121 KLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV 240
           NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV
Sbjct: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV 240

Query: 241 QGWPIPGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300
           QGWPIPGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL
Sbjct: 241 QGWPIPGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300

Query: 301 AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF 360
           AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF
Sbjct: 301 AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNK 420
           NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNK
Sbjct: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNK 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIF 449
           KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIF
Sbjct: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIF 480

BLAST of Cp4.1LG12g07350 vs. NCBI nr
Match: KAG6575609.1 (Glycerol kinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 859 bits (2219), Expect = 1.47e-312
Identity = 446/523 (85.28%), Postives = 447/523 (85.47%), Query Frame = 0

Query: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAG-------------- 60
           MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAG              
Sbjct: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAGWVEHDPMEILESVR 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  VCMAKAVDKATAAGYNVDNGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDARTSAICR 120

Query: 121 KLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIW 180
           KLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIW
Sbjct: 121 KLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV 240
           NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV
Sbjct: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV 240

Query: 241 QGWPIPGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300
           QGWPI GVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL
Sbjct: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300

Query: 301 AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF 360
           AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF
Sbjct: 301 AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNK 420
           NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDA EKGEVKN+
Sbjct: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAREKGEVKNE 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIF 449
           KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIF
Sbjct: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIF 480

BLAST of Cp4.1LG12g07350 vs. NCBI nr
Match: XP_022953937.1 (glycerol kinase [Cucurbita moschata] >KAG7014152.1 Glycerol kinase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 857 bits (2215), Expect = 5.99e-312
Identity = 445/523 (85.09%), Postives = 447/523 (85.47%), Query Frame = 0

Query: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAG-------------- 60
           MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAG              
Sbjct: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAGWVEHDPMEILESVR 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  VCMAKAVDKATAAGYNVDNGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDARTSAICR 120

Query: 121 KLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIW 180
           KLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIW
Sbjct: 121 KLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV 240
           NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV
Sbjct: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV 240

Query: 241 QGWPIPGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300
           QGWPI GVPISGCLGDQHAAMLGQACRKGEAKST+GTGAFILLNTGEEVVQSKHGLLTTL
Sbjct: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTFGTGAFILLNTGEEVVQSKHGLLTTL 300

Query: 301 AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF 360
           AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF
Sbjct: 301 AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNK 420
           NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDA EKGEVKN+
Sbjct: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAREKGEVKNE 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIF 449
           KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIF
Sbjct: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIF 480

BLAST of Cp4.1LG12g07350 vs. NCBI nr
Match: XP_022991575.1 (glycerol kinase [Cucurbita maxima])

HSP 1 Score: 855 bits (2210), Expect = 3.46e-311
Identity = 442/523 (84.51%), Postives = 446/523 (85.28%), Query Frame = 0

Query: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAG-------------- 60
           MAK EDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAG              
Sbjct: 1   MAKVEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAGWVEHDPMEILESVR 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  VCMAKAVDKATAAGYNVDNGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDARTSAICR 120

Query: 121 KLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIW 180
           KLENELSGGRTHFVGTCGLPISTYFSA+KLLWLMENVKDVNESIKKGDALFGTIDTWLIW
Sbjct: 121 KLENELSGGRTHFVGTCGLPISTYFSAMKLLWLMENVKDVNESIKKGDALFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV 240
           NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV
Sbjct: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV 240

Query: 241 QGWPIPGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300
           QGWPI GVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL
Sbjct: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300

Query: 301 AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF 360
           AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF
Sbjct: 301 AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNK 420
           NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGE KN+
Sbjct: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEAKNE 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIF 449
           KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRP+DIETTALGAAYAAGLAVGVWTEDEIF
Sbjct: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPSDIETTALGAAYAAGLAVGVWTEDEIF 480

BLAST of Cp4.1LG12g07350 vs. NCBI nr
Match: XP_008461141.1 (PREDICTED: glycerol kinase [Cucumis melo])

HSP 1 Score: 817 bits (2110), Expect = 5.89e-296
Identity = 422/523 (80.69%), Postives = 437/523 (83.56%), Query Frame = 0

Query: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAG-------------- 60
           MAKAEDVFVGSIDQGTTSTRFIIYDR SRPIGSHQ EFTQFYPEAG              
Sbjct: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRHSRPIGSHQAEFTQFYPEAGWVEHDPVEILESVK 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  VCMAKALDKATAAGYNVDNGLKAIGLTNQRETTLVWSKSSGSPLHNAIVWMDARTSSICR 120

Query: 121 KLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIW 180
           KLE ELSGGRTHFV TCGLPISTYFSAVKL+WLMENV+ V ESI+KGDALFGTIDTWLIW
Sbjct: 121 KLEKELSGGRTHFVDTCGLPISTYFSAVKLIWLMENVEAVRESIEKGDALFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV 240
           NLTGGVNGGLHVTDVSNASRTMLMNLKTL+WDKPTLETLGIPAQILPKIVSNSEVIGKI 
Sbjct: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLETLGIPAQILPKIVSNSEVIGKIS 240

Query: 241 QGWPIPGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300
           QGWPI GVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL
Sbjct: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300

Query: 301 AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF 360
           AFKLG+DA  NYALEGSIAIAGAAVQWLRDSLGIISSA+EIEELA QV+STGG+YFVPAF
Sbjct: 301 AFKLGRDAPTNYALEGSIAIAGAAVQWLRDSLGIISSAQEIEELALQVQSTGGVYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNK 420
           NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKN+
Sbjct: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNE 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIF 449
           KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVG+W++DEIF
Sbjct: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGIWSKDEIF 480

BLAST of Cp4.1LG12g07350 vs. ExPASy TrEMBL
Match: A0A6J1GPN0 (Glycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111456345 PE=3 SV=1)

HSP 1 Score: 857 bits (2215), Expect = 2.90e-312
Identity = 445/523 (85.09%), Postives = 447/523 (85.47%), Query Frame = 0

Query: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAG-------------- 60
           MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAG              
Sbjct: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAGWVEHDPMEILESVR 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  VCMAKAVDKATAAGYNVDNGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDARTSAICR 120

Query: 121 KLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIW 180
           KLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIW
Sbjct: 121 KLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV 240
           NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV
Sbjct: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV 240

Query: 241 QGWPIPGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300
           QGWPI GVPISGCLGDQHAAMLGQACRKGEAKST+GTGAFILLNTGEEVVQSKHGLLTTL
Sbjct: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTFGTGAFILLNTGEEVVQSKHGLLTTL 300

Query: 301 AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF 360
           AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF
Sbjct: 301 AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNK 420
           NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDA EKGEVKN+
Sbjct: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAREKGEVKNE 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIF 449
           KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIF
Sbjct: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIF 480

BLAST of Cp4.1LG12g07350 vs. ExPASy TrEMBL
Match: A0A6J1JTB9 (Glycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111488147 PE=3 SV=1)

HSP 1 Score: 855 bits (2210), Expect = 1.68e-311
Identity = 442/523 (84.51%), Postives = 446/523 (85.28%), Query Frame = 0

Query: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAG-------------- 60
           MAK EDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAG              
Sbjct: 1   MAKVEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAGWVEHDPMEILESVR 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  VCMAKAVDKATAAGYNVDNGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDARTSAICR 120

Query: 121 KLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIW 180
           KLENELSGGRTHFVGTCGLPISTYFSA+KLLWLMENVKDVNESIKKGDALFGTIDTWLIW
Sbjct: 121 KLENELSGGRTHFVGTCGLPISTYFSAMKLLWLMENVKDVNESIKKGDALFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV 240
           NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV
Sbjct: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV 240

Query: 241 QGWPIPGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300
           QGWPI GVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL
Sbjct: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300

Query: 301 AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF 360
           AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF
Sbjct: 301 AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNK 420
           NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGE KN+
Sbjct: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEAKNE 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIF 449
           KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRP+DIETTALGAAYAAGLAVGVWTEDEIF
Sbjct: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPSDIETTALGAAYAAGLAVGVWTEDEIF 480

BLAST of Cp4.1LG12g07350 vs. ExPASy TrEMBL
Match: A0A1S3CF92 (Glycerol kinase OS=Cucumis melo OX=3656 GN=LOC103499817 PE=3 SV=1)

HSP 1 Score: 817 bits (2110), Expect = 2.85e-296
Identity = 422/523 (80.69%), Postives = 437/523 (83.56%), Query Frame = 0

Query: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAG-------------- 60
           MAKAEDVFVGSIDQGTTSTRFIIYDR SRPIGSHQ EFTQFYPEAG              
Sbjct: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRHSRPIGSHQAEFTQFYPEAGWVEHDPVEILESVK 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  VCMAKALDKATAAGYNVDNGLKAIGLTNQRETTLVWSKSSGSPLHNAIVWMDARTSSICR 120

Query: 121 KLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIW 180
           KLE ELSGGRTHFV TCGLPISTYFSAVKL+WLMENV+ V ESI+KGDALFGTIDTWLIW
Sbjct: 121 KLEKELSGGRTHFVDTCGLPISTYFSAVKLIWLMENVEAVRESIEKGDALFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV 240
           NLTGGVNGGLHVTDVSNASRTMLMNLKTL+WDKPTLETLGIPAQILPKIVSNSEVIGKI 
Sbjct: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLETLGIPAQILPKIVSNSEVIGKIS 240

Query: 241 QGWPIPGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300
           QGWPI GVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL
Sbjct: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300

Query: 301 AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF 360
           AFKLG+DA  NYALEGSIAIAGAAVQWLRDSLGIISSA+EIEELA QV+STGG+YFVPAF
Sbjct: 301 AFKLGRDAPTNYALEGSIAIAGAAVQWLRDSLGIISSAQEIEELALQVQSTGGVYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNK 420
           NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKN+
Sbjct: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNE 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIF 449
           KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVG+W++DEIF
Sbjct: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGIWSKDEIF 480

BLAST of Cp4.1LG12g07350 vs. ExPASy TrEMBL
Match: A0A5A7UVL8 (Glycerol kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G002070 PE=3 SV=1)

HSP 1 Score: 815 bits (2105), Expect = 1.65e-295
Identity = 421/523 (80.50%), Postives = 436/523 (83.37%), Query Frame = 0

Query: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAG-------------- 60
           MAKAEDVFVGSIDQGTTSTRFIIYDR SRPIGSHQ EFTQFYPEAG              
Sbjct: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRHSRPIGSHQAEFTQFYPEAGWVEHDPVEILESVK 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  VCMAKALDKATAAGYNVDNGLKAIGLTNQRETTLVWSKSSGSPLHNAIVWMDARTSSICR 120

Query: 121 KLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIW 180
           KLE ELSGGRTHFV TCGLPISTYFSAVKL+WLMENV+ V ESI+KGDALFGTIDTWLIW
Sbjct: 121 KLEKELSGGRTHFVDTCGLPISTYFSAVKLIWLMENVEAVRESIEKGDALFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV 240
           NLTGGVNGGLHVTDVSNASRTMLMNLKTL+WDKPTLETLGIPAQILPKIVSNSEVIGKI 
Sbjct: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLETLGIPAQILPKIVSNSEVIGKIS 240

Query: 241 QGWPIPGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300
           QGWPI GVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL
Sbjct: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300

Query: 301 AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF 360
           AFKLG+DA  NYALEGSIAIAGAAVQWLRDSLGIISSA+EIEELA QV+STGG+YFVPAF
Sbjct: 301 AFKLGRDAPTNYALEGSIAIAGAAVQWLRDSLGIISSAQEIEELALQVQSTGGVYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNK 420
           NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKN+
Sbjct: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNE 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIF 449
           KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVG+W++DEIF
Sbjct: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGIWSKDEIF 480

BLAST of Cp4.1LG12g07350 vs. ExPASy TrEMBL
Match: A0A0A0KA32 (Glycerol kinase OS=Cucumis sativus OX=3659 GN=Csa_7G432280 PE=3 SV=1)

HSP 1 Score: 810 bits (2092), Expect = 1.57e-293
Identity = 420/523 (80.31%), Postives = 435/523 (83.17%), Query Frame = 0

Query: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAG-------------- 60
           MAKAEDVFVGSIDQGTTS+RFIIYDR SRPIGSHQ EFTQFYPEAG              
Sbjct: 1   MAKAEDVFVGSIDQGTTSSRFIIYDRHSRPIGSHQAEFTQFYPEAGWVEHDPVEILESVK 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  VCMAKALDKATAAGYNVDNGLKAIGLTNQRETTLVWSKSTGFPLHHAIVWMDARTSSICR 120

Query: 121 KLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIW 180
           KLE ELSGGRTHFV TCGLPISTYFSAVKL+WLMENV+ V ESIKK DALFGTIDTWLIW
Sbjct: 121 KLEKELSGGRTHFVDTCGLPISTYFSAVKLIWLMENVEAVRESIKKEDALFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV 240
           NLTGGVNGGLHVTDVSNASRTMLMNLKTL+WDKPTLETLGIPAQILPKIVSNSEVIGKI 
Sbjct: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLETLGIPAQILPKIVSNSEVIGKIS 240

Query: 241 QGWPIPGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300
           QGWPI GVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL
Sbjct: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300

Query: 301 AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF 360
           AFKLG DA  NYALEGSIAIAGAAVQWLRDSLGIISSA+EIE+LASQV+STGG+YFVPAF
Sbjct: 301 AFKLGPDAPTNYALEGSIAIAGAAVQWLRDSLGIISSAQEIEKLASQVQSTGGVYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNK 420
           NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDA EKGEVKN+
Sbjct: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAVEKGEVKNE 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIF 449
           KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVG+W++DEIF
Sbjct: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGIWSKDEIF 480

BLAST of Cp4.1LG12g07350 vs. TAIR 10
Match: AT1G80460.1 (Actin-like ATPase superfamily protein )

HSP 1 Score: 726.9 bits (1875), Expect = 1.0e-209
Identity = 366/523 (69.98%), Postives = 409/523 (78.20%), Query Frame = 0

Query: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAG-------------- 60
           MAK E+ F+GSIDQGTTSTRFIIYD  +RP+ SHQVEFTQFYPEAG              
Sbjct: 1   MAK-ENGFIGSIDQGTTSTRFIIYDHDARPVASHQVEFTQFYPEAGWVEHDPMEILESVK 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  VCIAKALDKATADGHNVDGGLKAIGLTDQRETTVVWSKSTGLPLHKAIVWMDARTSSICR 120

Query: 121 KLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIW 180
           +LE ELSGGR+HFV +CGLPISTYFSA+KLLWLMENV DV ++IKKGDA+FGTIDTWLIW
Sbjct: 121 RLEKELSGGRSHFVESCGLPISTYFSAMKLLWLMENVDDVKDAIKKGDAIFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV 240
           N+TGG+NGGLHVTDV+NASRTMLMNLKTL WD+ TL+TLGIPA+ILPKIVSNSEVIG+I 
Sbjct: 181 NMTGGINGGLHVTDVTNASRTMLMNLKTLSWDQDTLKTLGIPAEILPKIVSNSEVIGEIC 240

Query: 241 QGWPIPGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300
           +GWPIPG+ I+GCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGE  ++S HGLLTTL
Sbjct: 241 KGWPIPGIKIAGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEVPIKSGHGLLTTL 300

Query: 301 AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF 360
           A+KLG  A+ NYALEGSIAIAGAAVQWLRDSLGII SA EIE+LA+ V STGG+YFVPAF
Sbjct: 301 AYKLGPQAQTNYALEGSIAIAGAAVQWLRDSLGIIKSASEIEDLAAMVDSTGGVYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNK 420
           NGLFAPWWR+DARGVCIGITRFT+K+HIARAVLESMCFQVKDVLDSM+KDAGEKG + N 
Sbjct: 361 NGLFAPWWREDARGVCIGITRFTNKSHIARAVLESMCFQVKDVLDSMNKDAGEKGSLNNG 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIF 450
           KGEFLLRVDGGAT NNLLMQIQADL+GSPVVRP DIETTALGAAYAAGLAVG W E +IF
Sbjct: 421 KGEFLLRVDGGATANNLLMQIQADLMGSPVVRPVDIETTALGAAYAAGLAVGFWKEADIF 480

BLAST of Cp4.1LG12g07350 vs. TAIR 10
Match: AT1G80460.2 (Actin-like ATPase superfamily protein )

HSP 1 Score: 636.7 bits (1641), Expect = 1.4e-182
Identity = 329/523 (62.91%), Postives = 371/523 (70.94%), Query Frame = 0

Query: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAG-------------- 60
           MAK E+ F+GSIDQGTTSTRFIIYD  +RP+ SHQVEFTQFYPEAG              
Sbjct: 1   MAK-ENGFIGSIDQGTTSTRFIIYDHDARPVASHQVEFTQFYPEAGWVEHDPMEILESVK 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  VCIAKALDKATADGHNVDGGLKAIGLTDQRETTVVWSKSTGLPLHKAIVWMDARTSSICR 120

Query: 121 KLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIW 180
           +LE ELSGGR+HFV +CGLPISTYFSA+KLLWLMENV DV ++IKKGDA+FGTIDTWLIW
Sbjct: 121 RLEKELSGGRSHFVESCGLPISTYFSAMKLLWLMENVDDVKDAIKKGDAIFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV 240
           N+TGG+NGGLHVTDV+NASRTMLMNLKTL WD+ TL+TLGIPA+ILPKIVSNSEVIG+I 
Sbjct: 181 NMTGGINGGLHVTDVTNASRTMLMNLKTLSWDQDTLKTLGIPAEILPKIVSNSEVIGEIC 240

Query: 241 QGWPIPGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300
           +GWPIPG+ I+GCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGE  ++S HGLLTTL
Sbjct: 241 KGWPIPGIKIAGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEVPIKSGHGLLTTL 300

Query: 301 AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF 360
           A+KLG  A+ NYALEGSIAIAGAAVQWLRDSLGII SA EIE+LA+ V STGG+YFVPAF
Sbjct: 301 AYKLGPQAQTNYALEGSIAIAGAAVQWLRDSLGIIKSASEIEDLAAMVDSTGGVYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNK 420
           NGLFAPWWR+DARGVCIGITRFT+K+HIARAVLESMCFQVKDVLDSM+KDAGEKG + N 
Sbjct: 361 NGLFAPWWREDARGVCIGITRFTNKSHIARAVLESMCFQVKDVLDSMNKDAGEKGSLNNG 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIF 450
           KGEFLLRVD                                        AVG W E +IF
Sbjct: 421 KGEFLLRVD----------------------------------------AVGFWKEADIF 480

BLAST of Cp4.1LG12g07350 vs. TAIR 10
Match: AT4G30310.3 (FGGY family of carbohydrate kinase )

HSP 1 Score: 46.2 bits (108), Expect = 8.0e-05
Identity = 31/123 (25.20%), Postives = 62/123 (50.41%), Query Frame = 0

Query: 277 TGGIYFVPAFNGLFAPWWRDDARGVCIGITRFTSKAHIA---RAVLESMCFQVKDVLDSM 336
           T  ++ +P F+G  +P    +++GV  G++  TS+  +A    A ++ + +  + +++  
Sbjct: 279 TSDMHILPDFHGNRSPVADPNSKGVIFGMSLDTSEKQLALLYLATIQGIAYGTRHIVEHC 338

Query: 337 HKDAGEKGEVKNKKGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAA 396
           +   G K +         L   GG + N L +Q  AD++G P++ P + E+  LGAA   
Sbjct: 339 NTH-GHKIDT--------LLACGGLSKNPLFIQEHADIVGCPIILPRESESVLLGAAILG 392

BLAST of Cp4.1LG12g07350 vs. TAIR 10
Match: AT4G30310.2 (FGGY family of carbohydrate kinase )

HSP 1 Score: 46.2 bits (108), Expect = 8.0e-05
Identity = 31/123 (25.20%), Postives = 62/123 (50.41%), Query Frame = 0

Query: 277 TGGIYFVPAFNGLFAPWWRDDARGVCIGITRFTSKAHIA---RAVLESMCFQVKDVLDSM 336
           T  ++ +P F+G  +P    +++GV  G++  TS+  +A    A ++ + +  + +++  
Sbjct: 407 TSDMHILPDFHGNRSPVADPNSKGVIFGMSLDTSEKQLALLYLATIQGIAYGTRHIVEHC 466

Query: 337 HKDAGEKGEVKNKKGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAA 396
           +   G K +         L   GG + N L +Q  AD++G P++ P + E+  LGAA   
Sbjct: 467 NTH-GHKIDT--------LLACGGLSKNPLFIQEHADIVGCPIILPRESESVLLGAAILG 520

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M8L41.4e-20869.98Glycerol kinase OS=Arabidopsis thaliana OX=3702 GN=GLPK PE=1 SV=1[more]
B8GC514.6e-12247.92Glycerol kinase OS=Chloroflexus aggregans (strain MD-66 / DSM 9485) OX=326427 GN... [more]
B9LD342.5e-12047.72Glycerol kinase OS=Chloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400... [more]
A9WJ214.3e-12047.72Glycerol kinase OS=Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-... [more]
A6WXV21.2e-11947.92Glycerol kinase OS=Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / JCM 210... [more]
Match NameE-valueIdentityDescription
XP_023548708.11.47e-31585.85glycerol kinase [Cucurbita pepo subsp. pepo][more]
KAG6575609.11.47e-31285.28Glycerol kinase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022953937.15.99e-31285.09glycerol kinase [Cucurbita moschata] >KAG7014152.1 Glycerol kinase [Cucurbita ar... [more]
XP_022991575.13.46e-31184.51glycerol kinase [Cucurbita maxima][more]
XP_008461141.15.89e-29680.69PREDICTED: glycerol kinase [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1GPN02.90e-31285.09Glycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111456345 PE=3 SV=1[more]
A0A6J1JTB91.68e-31184.51Glycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111488147 PE=3 SV=1[more]
A0A1S3CF922.85e-29680.69Glycerol kinase OS=Cucumis melo OX=3656 GN=LOC103499817 PE=3 SV=1[more]
A0A5A7UVL81.65e-29580.50Glycerol kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G0020... [more]
A0A0A0KA321.57e-29380.31Glycerol kinase OS=Cucumis sativus OX=3659 GN=Csa_7G432280 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G80460.11.0e-20969.98Actin-like ATPase superfamily protein [more]
AT1G80460.21.4e-18262.91Actin-like ATPase superfamily protein [more]
AT4G30310.38.0e-0525.20FGGY family of carbohydrate kinase [more]
AT4G30310.28.0e-0525.20FGGY family of carbohydrate kinase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018485Carbohydrate kinase, FGGY, C-terminalPFAMPF02782FGGY_Ccoord: 197..396
e-value: 3.3E-59
score: 200.0
NoneNo IPR availableGENE3D3.30.420.40coord: 191..444
e-value: 5.6E-90
score: 302.7
NoneNo IPR availableGENE3D3.30.420.40coord: 51..190
e-value: 7.0E-45
score: 155.7
NoneNo IPR availableGENE3D3.30.420.40coord: 3..50
e-value: 3.7E-7
score: 32.1
NoneNo IPR availablePANTHERPTHR10196:SF91SUBFAMILY NOT NAMEDcoord: 47..445
NoneNo IPR availablePANTHERPTHR10196SUGAR KINASEcoord: 47..445
NoneNo IPR availablePANTHERPTHR10196SUGAR KINASEcoord: 4..50
NoneNo IPR availablePANTHERPTHR10196:SF91SUBFAMILY NOT NAMEDcoord: 4..50
IPR018484Carbohydrate kinase, FGGY, N-terminalPFAMPF00370FGGY_Ncoord: 55..189
e-value: 3.6E-34
score: 118.4
IPR000577Carbohydrate kinase, FGGYPIRSFPIRSF000538GlpKcoord: 49..448
e-value: 3.6E-108
score: 360.0
coord: 7..54
e-value: 4.7E-6
score: 23.1
IPR018483Carbohydrate kinase, FGGY, conserved sitePROSITEPS00933FGGY_KINASES_1coord: 70..82
IPR043129ATPase, nucleotide binding domainSUPERFAMILY53067Actin-like ATPase domaincoord: 192..442
IPR043129ATPase, nucleotide binding domainSUPERFAMILY53067Actin-like ATPase domaincoord: 9..192

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG12g07350.1Cp4.1LG12g07350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046167 glycerol-3-phosphate biosynthetic process
biological_process GO:0019563 glycerol catabolic process
biological_process GO:0016310 phosphorylation
biological_process GO:0006641 triglyceride metabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005524 ATP binding
molecular_function GO:0004370 glycerol kinase activity
molecular_function GO:0016773 phosphotransferase activity, alcohol group as acceptor