Cp4.1LG12g07100 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG12g07100
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionmitogen-activated protein kinase kinase kinase 17-like
LocationCp4.1LG12: 7117202 .. 7118898 (+)
RNA-Seq ExpressionCp4.1LG12g07100
SyntenyCp4.1LG12g07100
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTCTGAAGCGATCAACCGCTGAATTCAGCATTTTCCCTCCGGTAATGGCGGTCAAATCTGCGGAAGTTTCCGTTTCTAAATCCCTTCAGAAGGAGAAACAAATTTATGATAACTTGAAAGGATGCAATTCCTTGATCCAATGCTTTGGAGAAGAAATTACTACCGATCGCAACGGTCGAATGATTTATAATTTGCTGTTGGAGATCGCCGTGGGTGGATCTCTCGCTCATCGCATCAATCACACAGGTTTCTTATTTCAATCACCACTCTCATTGTTTATCATTTGTTTTAGATTCTAATTTTTTCGTCACCCGATTTTACATGAATACTTCAAACACACGAAATTTAACTAACTCAAAAAATCATTCAAACATAAACTAGAAATTTAAATACAAACTTTCGTTTTTTAAAATAAAATTAGAAATTTAAATATAAACGTTAGCATCACATTTCAATTTTCATATCAACCAAAATTTTCTACTCAATTTAAGGTAGGTTACTACTGTGTCATGAACATTGAATGAACTTATATTTAAATTTCGTAACTTTTTTCTTAAAACACAACCATGAACTTATATTTCAAAAAGACCTATAAAAAAAATATTTAAATTAAAAACATTATATTCTAGCCTAAGCGTAACAAAATAAATATGGATTAAGTATTTCAAAAATAGACTAAAACTAAATAAAATAAAATAAAAATATTTAAATTAAAAACATTATATTCTAATGTAAGGCTGAAGAAAATAAATACCACTACCTATGTATGTGTATAGGTAAGACTAAATAAAATTAAGAAAATATTTAAATTAAAAACTTAAGCCTAAGCAAATAAATATCACTATCGTATGTATGTGTCATGATTTTGAATTTTGCTGGTCATCCTTGCACCGGGGTCATGCTAATATTTGGGTATTTGGGTATGGTATATCATGTTTAGATTTTAATGAAAGAAGTTAAAATATTTTGATTTTGTTAAGAAATTTGTTGGTGAATTAATGTAGGTGGAAAGGGATTAGCAGAAAACGAGGTTGGGAGGTACACGAAGACAATAATCAAAGGCCTAATTCACATCCACAGAAGAGGGTACGTTCACTGTGATTTGAAGCCTGCAAATATCTTGTTGCTGCCCACCAACACAGCCAAGGGCGCCCGATTGGTGGCCAAGATTTGTGATTTGGGGTTGGCCAGGAGAGGCAGCGAGACAAGGCATGGCAATTGTTTTGGAGGGACGCTGTCGTACATGGCGCCAGAGACATTGATTCACCGCGTGCAAGAGTCGCCCAGTGATATTTGGGCACTTGGCTGCCTTGTGCTGGAAATGCTCACCGGAAACCGCCCCTGGGCCGCCACGGACAAGGCTGGGATAGTGAAAAGCATTACTCAGAATTGCGGAATGCCGGAGATTCCAGAAGGGGTGTCGAAAGAGGCAAGTGGGTTTTTGAAGAATTGCTTTGTGAGGAATCCGGAGTACAGATTCACGGCGGAGATGCTTATGACTGTGCCGTTTGTGGCGGCGGCGGAGGAGGAGGAGGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGATTTTATGAGAGCTCTAACATTTGTGAGGAAGAGGAGTAGAAGCTGTAGAAAAAGGCAAAGAATAGTTCCAATTGAAGCTATGCGAGTAATTAATATTAATCAATGA

mRNA sequence

ATGGCTTCTCTGAAGCGATCAACCGCTGAATTCAGCATTTTCCCTCCGGTAATGGCGGTCAAATCTGCGGAAGTTTCCGTTTCTAAATCCCTTCAGAAGGAGAAACAAATTTATGATAACTTGAAAGGATGCAATTCCTTGATCCAATGCTTTGGAGAAGAAATTACTACCGATCGCAACGGTCGAATGATTTATAATTTGCTGTTGGAGATCGCCGTGGGTGGATCTCTCGCTCATCGCATCAATCACACAGGTGGAAAGGGATTAGCAGAAAACGAGGTTGGGAGGTACACGAAGACAATAATCAAAGGCCTAATTCACATCCACAGAAGAGGGTACGTTCACTGTGATTTGAAGCCTGCAAATATCTTGTTGCTGCCCACCAACACAGCCAAGGGCGCCCGATTGGTGGCCAAGATTTGTGATTTGGGGTTGGCCAGGAGAGGCAGCGAGACAAGGCATGGCAATTGTTTTGGAGGGACGCTGTCGTACATGGCGCCAGAGACATTGATTCACCGCGTGCAAGAGTCGCCCAGTGATATTTGGGCACTTGGCTGCCTTGTGCTGGAAATGCTCACCGGAAACCGCCCCTGGGCCGCCACGGACAAGGCTGGGATAGTGAAAAGCATTACTCAGAATTGCGGAATGCCGGAGATTCCAGAAGGGGTGTCGAAAGAGGCAAGTGGGTTTTTGAAGAATTGCTTTGTGAGGAATCCGGAGTACAGATTCACGGCGGAGATGCTTATGACTGTGCCGTTTGTGGCGGCGGCGGAGGAGGAGGAGGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGATTTTATGAGAGCTCTAACATTTGTGAGGAAGAGGAGTAGAAGCTGTAGAAAAAGGCAAAGAATAGTTCCAATTGAAGCTATGCGAGTAATTAATATTAATCAATGA

Coding sequence (CDS)

ATGGCTTCTCTGAAGCGATCAACCGCTGAATTCAGCATTTTCCCTCCGGTAATGGCGGTCAAATCTGCGGAAGTTTCCGTTTCTAAATCCCTTCAGAAGGAGAAACAAATTTATGATAACTTGAAAGGATGCAATTCCTTGATCCAATGCTTTGGAGAAGAAATTACTACCGATCGCAACGGTCGAATGATTTATAATTTGCTGTTGGAGATCGCCGTGGGTGGATCTCTCGCTCATCGCATCAATCACACAGGTGGAAAGGGATTAGCAGAAAACGAGGTTGGGAGGTACACGAAGACAATAATCAAAGGCCTAATTCACATCCACAGAAGAGGGTACGTTCACTGTGATTTGAAGCCTGCAAATATCTTGTTGCTGCCCACCAACACAGCCAAGGGCGCCCGATTGGTGGCCAAGATTTGTGATTTGGGGTTGGCCAGGAGAGGCAGCGAGACAAGGCATGGCAATTGTTTTGGAGGGACGCTGTCGTACATGGCGCCAGAGACATTGATTCACCGCGTGCAAGAGTCGCCCAGTGATATTTGGGCACTTGGCTGCCTTGTGCTGGAAATGCTCACCGGAAACCGCCCCTGGGCCGCCACGGACAAGGCTGGGATAGTGAAAAGCATTACTCAGAATTGCGGAATGCCGGAGATTCCAGAAGGGGTGTCGAAAGAGGCAAGTGGGTTTTTGAAGAATTGCTTTGTGAGGAATCCGGAGTACAGATTCACGGCGGAGATGCTTATGACTGTGCCGTTTGTGGCGGCGGCGGAGGAGGAGGAGGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGATTTTATGAGAGCTCTAACATTTGTGAGGAAGAGGAGTAGAAGCTGTAGAAAAAGGCAAAGAATAGTTCCAATTGAAGCTATGCGAGTAATTAATATTAATCAATGA

Protein sequence

MASLKRSTAEFSIFPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRNGRMIYNLLLEIAVGGSLAHRINHTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKPANILLLPTNTAKGARLVAKICDLGLARRGSETRHGNCFGGTLSYMAPETLIHRVQESPSDIWALGCLVLEMLTGNRPWAATDKAGIVKSITQNCGMPEIPEGVSKEASGFLKNCFVRNPEYRFTAEMLMTVPFVAAAEEEEEEEEEEEEEEEEEEEEDFMRALTFVRKRSRSCRKRQRIVPIEAMRVININQ
Homology
BLAST of Cp4.1LG12g07100 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 2.7e-41
Identity = 106/269 (39.41%), Postives = 156/269 (57.99%), Query Frame = 0

Query: 14  FPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRNGRMIYNLLLEIAV 73
           FP ++AVKS +   + SL  EK + D+L  C  +I+C+GE+ T + NG  ++NLLLE A 
Sbjct: 29  FPALIAVKSTDAYGAASLSNEKSVLDSLGDCPEIIRCYGEDSTVE-NGEEMHNLLLEYAS 88

Query: 74  GGSLAHRINHTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKPANILLLPTNTAKG 133
            GSLA  +   GG+GL E+ V R+T ++++GL HIH +G+ HCD+K ANILL    +   
Sbjct: 89  RGSLASYMKKLGGEGLPESTVRRHTGSVLRGLRHIHAKGFAHCDIKLANILLFNDGS--- 148

Query: 134 ARLVAKICDLGLARR--GSET--RHGNCFGGTLSYMAPETLIHRVQESPSDIWALGCLVL 193
                KI D GLA R  G  T  R      GT  YMAPE +      S +D+WALGC V+
Sbjct: 149 ----VKIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPECVNDNEYGSAADVWALGCAVV 208

Query: 194 EMLTGNRPWAATDKA---GIVKSITQNCGMPEIPEGVSKEASGFLKNCFVRNPEYRFTAE 253
           EM +G   W+  + +    ++  I     +P+IPE +S+E   FL  CFV++P  R+TAE
Sbjct: 209 EMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSEEGKDFLSKCFVKDPAKRWTAE 268

Query: 254 MLMTVPFVAAAEEEEEEEEEEEEEEEEEE 276
           ML+   FV    E++  E    + ++E++
Sbjct: 269 MLLNHSFVTIDLEDDHRENFVVKVKDEDK 289

BLAST of Cp4.1LG12g07100 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.2e-31
Identity = 103/293 (35.15%), Postives = 150/293 (51.19%), Query Frame = 0

Query: 17  VMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRNGRMIYNLLLEIAVGGS 76
           ++AVKS+EV  S+ LQ+E +I  +L     +I   G E   + NG ++YNLL+E A  G+
Sbjct: 28  ILAVKSSEVHRSEFLQREAKILSSLSS-PYVIGYRGSETKRESNGVVMYNLLMEYAPYGT 87

Query: 77  LAHRINHTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKPANILLLPTNTAKGARL 136
           L       GG+ + E  V +YT+ I+KGL +IH +G VHCD+K +N+++    + KG   
Sbjct: 88  LTDAAAKDGGR-VDETRVVKYTRDILKGLEYIHSKGIVHCDVKGSNVVI----SEKGE-- 147

Query: 137 VAKICDLGLARRGSETRHGNCFGGTLSYMAPETLIHRVQESPSDIWALGCLVLEMLTGNR 196
            AKI D G A+R           GT ++MAPE      Q   SDIWA+GC ++EM+TG+ 
Sbjct: 148 -AKIADFGCAKRVDPVFESPVM-GTPAFMAPEVARGEKQGKESDIWAVGCTMIEMVTGSP 207

Query: 197 PWAATDKAGIVKSITQNCG----MPEIPEGVSKEASGFLKNCFVRNPEYRFTAEMLMTVP 256
           PW   D      S+    G     PE+P  +++EA  FL+ C  R    R+TA  L+  P
Sbjct: 208 PWTKADSREDPVSVLYRVGYSSETPELPCLLAEEAKDFLEKCLKREANERWTATQLLNHP 267

Query: 257 FVAAAEEEE------------------------EEEEEEEEEEEEEEEEDFMR 282
           F+    + E                        EEEEEEE EE +++  D  R
Sbjct: 268 FLTTKPDIEPVLVPGLISNSPTSVTDQTFWRSVEEEEEEETEEIQKDSRDLDR 310

BLAST of Cp4.1LG12g07100 vs. ExPASy Swiss-Prot
Match: P28829 (Protein kinase byr2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=byr2 PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 4.3e-26
Identity = 85/251 (33.86%), Postives = 127/251 (50.60%), Query Frame = 0

Query: 17  VMAVKSAEV-SVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRNGRMI----YNLLLEI 76
           +MAVK   + SVS+S  +  ++ D L G  +L+Q    E      G  +     N+ LE 
Sbjct: 419 LMAVKQVILDSVSESKDRHAKLLDALAGEIALLQELSHEHIVQYLGSNLNSDHLNIFLEY 478

Query: 77  AVGGSLAHRINHTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKPANILLLPTNTA 136
             GGS+A  +   G     E  V  + K  +KGL ++H RG VH D+K ANIL+   N  
Sbjct: 479 VPGGSVAGLLTMYG--SFEETLVKNFIKQTLKGLEYLHSRGIVHRDIKGANILV--DNKG 538

Query: 137 KGARLVAKICDLGLARR----GSETRHGNC---FGGTLSYMAPETLIHRVQESPSDIWAL 196
           K      KI D G++++     + T+ G     F G+  +MAPE +   +    +DIW+L
Sbjct: 539 K-----IKISDFGISKKLELNSTSTKTGGARPSFQGSSFWMAPEVVKQTMHTEKTDIWSL 598

Query: 197 GCLVLEMLTGNRPWAATDKAGIVKSITQNCGMPEIPEGVSKEASGFLKNCFVRNPEYRFT 256
           GCLV+EMLT   P+   D+   +  I +N  +PE P  +S  A  FL+  F  +   R T
Sbjct: 599 GCLVIEMLTSKHPYPNCDQMQAIFRIGENI-LPEFPSNISSSAIDFLEKTFAIDCNLRPT 658

BLAST of Cp4.1LG12g07100 vs. ExPASy Swiss-Prot
Match: Q9C5H5 (Mitogen-activated protein kinase kinase kinase 5 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK5 PE=1 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 5.6e-26
Identity = 85/253 (33.60%), Postives = 131/253 (51.78%), Query Frame = 0

Query: 10  EFSIFPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRNGRMIYNLLL 69
           E  +FP     KSAE    K L++E ++  NL+  N ++Q FG E   DR     + + L
Sbjct: 376 EVELFPD--DPKSAE--CIKQLEQEIKLLSNLQHPN-IVQYFGSETVEDR-----FFIYL 435

Query: 70  EIAVGGSLAHRI-NHTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKPANILLLPT 129
           E    GS+   I +H G   + E+ V  +T+ I+ GL ++H +  VH D+K AN+L+   
Sbjct: 436 EYVHPGSINKYIRDHCG--TMTESVVRNFTRHILSGLAYLHNKKTVHRDIKGANLLV--- 495

Query: 130 NTAKGARLVAKICDLGLARRGSETRHGNCFGGTLSYMAPETLIHRVQESPS-------DI 189
                A  V K+ D G+A+  +  R      G+  +MAPE +   +Q+  +       DI
Sbjct: 496 ----DASGVVKLADFGMAKHLTGQRADLSLKGSPYWMAPELMQAVMQKDSNPDLAFAVDI 555

Query: 190 WALGCLVLEMLTGNRPWAATDKAGIVKSITQNCGMPEIPEGVSKEASGFLKNCFVRNPEY 249
           W+LGC ++EM TG  PW+  + A  +  + ++   P IPE +S E   FL+ CF RNP  
Sbjct: 556 WSLGCTIIEMFTGKPPWSEFEGAAAMFKVMRD--SPPIPESMSPEGKDFLRLCFQRNPAE 607

Query: 250 RFTAEMLMTVPFV 255
           R TA ML+   F+
Sbjct: 616 RPTASMLLEHRFL 607

BLAST of Cp4.1LG12g07100 vs. ExPASy Swiss-Prot
Match: Q01389 (Serine/threonine-protein kinase BCK1/SLK1/SSP31 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=BCK1 PE=1 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 3.1e-24
Identity = 83/255 (32.55%), Postives = 122/255 (47.84%), Query Frame = 0

Query: 17   VMAVKSAEV-----------SVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRNGRMIY 76
            +MAVK  EV           S  ++L+ E     +L   N ++Q  G E     N   IY
Sbjct: 1200 MMAVKQVEVPKYSSQNEAILSTVEALRSEVSTLKDLDHLN-IVQYLGFE-----NKNNIY 1259

Query: 77   NLLLEIAVGGSLAHRINHTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKPANILL 136
            +L LE   GGS+   I   G     E  +   T  ++KGL ++H +G +H D+K  N+LL
Sbjct: 1260 SLFLEYVAGGSVGSLIRMYG--RFDEPLIKHLTTQVLKGLAYLHSKGILHRDMKADNLLL 1319

Query: 137  LPTNTAKGARLVAKICDLGLARRGSE--TRHGNCFGGTLSYMAPETLIHRVQESPS-DIW 196
                       + KI D G++R+  +  +       GT+ +MAPE +  +   S   DIW
Sbjct: 1320 DQDG-------ICKISDFGISRKSKDIYSNSDMTMRGTVFWMAPEMVDTKQGYSAKVDIW 1379

Query: 197  ALGCLVLEMLTGNRPWAATDKAGIVKSITQNCGMPEIPEG----VSKEASGFLKNCFVRN 254
            +LGC+VLEM  G RPW+  +    +  I ++   P IPE     +S+    FL  CF  N
Sbjct: 1380 SLGCIVLEMFAGKRPWSNLEVVAAMFKIGKSKSAPPIPEDTLPLISQIGRNFLDACFEIN 1439

BLAST of Cp4.1LG12g07100 vs. NCBI nr
Match: KAG7014197.1 (Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 567 bits (1462), Expect = 2.41e-202
Identity = 294/309 (95.15%), Postives = 299/309 (96.76%), Query Frame = 0

Query: 1   MASLKRSTAEFSIFPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRN 60
           MASLKRSTAEFSIFPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRN
Sbjct: 47  MASLKRSTAEFSIFPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRN 106

Query: 61  GRMIYNLLLEIAVGGSLAHRINHTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKP 120
           G MIYNLLLEIA+GGSLAHRIN+TGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKP
Sbjct: 107 GPMIYNLLLEIALGGSLAHRINNTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKP 166

Query: 121 ANILLLPTNTAKGARLVAKICDLGLARRGSETRHGNCFGGTLSYMAPETLIHRVQESPSD 180
           ANILLLPTNTAKG RLVAKICDLGLARR SETRHGNCFGGTLSYMAPETLIHRVQESPSD
Sbjct: 167 ANILLLPTNTAKGGRLVAKICDLGLARRTSETRHGNCFGGTLSYMAPETLIHRVQESPSD 226

Query: 181 IWALGCLVLEMLTGNRPWAATDKAGIVKSITQNCGMPEIPEGVSKEASGFLKNCFVRNPE 240
           IWALGCLVLEMLTGNRPWAATD+AGIVKSIT+N GMPEIPEGVSKEASGFLKNCFVRNPE
Sbjct: 227 IWALGCLVLEMLTGNRPWAATDEAGIVKSITENDGMPEIPEGVSKEASGFLKNCFVRNPE 286

Query: 241 YRFTAEMLMTVPFVAAAEEEEEEEEEEEEEEEEEEEEDFMRALTFVRKRSRSCRKRQRIV 300
           YRFTAEMLMTVPFVAA     EEEEEEEEEEEEEEEEDFMRALTFVRKRSRSCRKRQRIV
Sbjct: 287 YRFTAEMLMTVPFVAA-----EEEEEEEEEEEEEEEEDFMRALTFVRKRSRSCRKRQRIV 346

Query: 301 PIEAMRVIN 309
           P EA+RVIN
Sbjct: 347 PTEAVRVIN 350

BLAST of Cp4.1LG12g07100 vs. NCBI nr
Match: XP_022953922.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata])

HSP 1 Score: 551 bits (1420), Expect = 4.18e-196
Identity = 286/309 (92.56%), Postives = 292/309 (94.50%), Query Frame = 0

Query: 1   MASLKRSTAEFSIFPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRN 60
           MASLKRS  EFSIFPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRN
Sbjct: 43  MASLKRS--EFSIFPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRN 102

Query: 61  GRMIYNLLLEIAVGGSLAHRINHTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKP 120
           GRMIYNL LEIA+GGSLAHRIN+ GGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKP
Sbjct: 103 GRMIYNLRLEIALGGSLAHRINNIGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKP 162

Query: 121 ANILLLPTNTAKGARLVAKICDLGLARRGSETRHGNCFGGTLSYMAPETLIHRVQESPSD 180
           ANILLLPTNTAKG RLVAKICDLGLARR SETRHGNCFGGTLSYMAPETLIHRVQESPSD
Sbjct: 163 ANILLLPTNTAKGGRLVAKICDLGLARRTSETRHGNCFGGTLSYMAPETLIHRVQESPSD 222

Query: 181 IWALGCLVLEMLTGNRPWAATDKAGIVKSITQNCGMPEIPEGVSKEASGFLKNCFVRNPE 240
           IWALGCLVLEMLTGNRPWAATD+AGIVKSIT+N GMPEIPEGVSK+ASGFLKNCFVRNPE
Sbjct: 223 IWALGCLVLEMLTGNRPWAATDEAGIVKSITENDGMPEIPEGVSKDASGFLKNCFVRNPE 282

Query: 241 YRFTAEMLMTVPFVAAAEEEEEEEEEEEEEEEEEEEEDFMRALTFVRKRSRSCRKRQRIV 300
           YRFTAEMLMTVPFVAAAEEEEEEEEEEE         DFMRALTFVRKRSRSCRKRQRIV
Sbjct: 283 YRFTAEMLMTVPFVAAAEEEEEEEEEEE---------DFMRALTFVRKRSRSCRKRQRIV 340

Query: 301 PIEAMRVIN 309
           P EA+RVIN
Sbjct: 343 PTEAVRVIN 340

BLAST of Cp4.1LG12g07100 vs. NCBI nr
Match: XP_023548452.1 (mitogen-activated protein kinase kinase kinase 17-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 493 bits (1269), Expect = 1.04e-174
Identity = 244/244 (100.00%), Postives = 244/244 (100.00%), Query Frame = 0

Query: 18  MAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRNGRMIYNLLLEIAVGGSL 77
           MAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRNGRMIYNLLLEIAVGGSL
Sbjct: 1   MAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRNGRMIYNLLLEIAVGGSL 60

Query: 78  AHRINHTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKPANILLLPTNTAKGARLV 137
           AHRINHTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKPANILLLPTNTAKGARLV
Sbjct: 61  AHRINHTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKPANILLLPTNTAKGARLV 120

Query: 138 AKICDLGLARRGSETRHGNCFGGTLSYMAPETLIHRVQESPSDIWALGCLVLEMLTGNRP 197
           AKICDLGLARRGSETRHGNCFGGTLSYMAPETLIHRVQESPSDIWALGCLVLEMLTGNRP
Sbjct: 121 AKICDLGLARRGSETRHGNCFGGTLSYMAPETLIHRVQESPSDIWALGCLVLEMLTGNRP 180

Query: 198 WAATDKAGIVKSITQNCGMPEIPEGVSKEASGFLKNCFVRNPEYRFTAEMLMTVPFVAAA 257
           WAATDKAGIVKSITQNCGMPEIPEGVSKEASGFLKNCFVRNPEYRFTAEMLMTVPFVAAA
Sbjct: 181 WAATDKAGIVKSITQNCGMPEIPEGVSKEASGFLKNCFVRNPEYRFTAEMLMTVPFVAAA 240

Query: 258 EEEE 261
           EEEE
Sbjct: 241 EEEE 244

BLAST of Cp4.1LG12g07100 vs. NCBI nr
Match: KAG6575645.1 (Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 493 bits (1269), Expect = 2.48e-173
Identity = 264/309 (85.44%), Postives = 269/309 (87.06%), Query Frame = 0

Query: 1   MASLKRSTAEFSIFPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRN 60
           MASLKRSTAEFSIFPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRN
Sbjct: 52  MASLKRSTAEFSIFPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRN 111

Query: 61  GRMIYNLLLEIAVGGSLAHRINHTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKP 120
            RMIYNLLLEIA+                   EV      IIKGLIHIHRRGYVHCDLKP
Sbjct: 112 SRMIYNLLLEIAL-------------------EV-----KIIKGLIHIHRRGYVHCDLKP 171

Query: 121 ANILLLPTNTAKGARLVAKICDLGLARRGSETRHGNCFGGTLSYMAPETLIHRVQESPSD 180
           ANILLLPTNTAKG RLVAKICDLGLARR SETRHGNCFGGTLSYMAPETLIHRVQESPSD
Sbjct: 172 ANILLLPTNTAKGGRLVAKICDLGLARRTSETRHGNCFGGTLSYMAPETLIHRVQESPSD 231

Query: 181 IWALGCLVLEMLTGNRPWAATDKAGIVKSITQNCGMPEIPEGVSKEASGFLKNCFVRNPE 240
           IWALGCLVLEMLTGNRPWAATD+AGIVKSIT+N GMPEIPEGVSK+ASGFLKNCFVRNPE
Sbjct: 232 IWALGCLVLEMLTGNRPWAATDEAGIVKSITENDGMPEIPEGVSKDASGFLKNCFVRNPE 291

Query: 241 YRFTAEMLMTVPFVAAAEEEEEEEEEEEEEEEEEEEEDFMRALTFVRKRSRSCRKRQRIV 300
           YRFTAEMLMTVPFVAAAEEEEEEEEEE          DFMRALTFVRKRSRSCRKRQRIV
Sbjct: 292 YRFTAEMLMTVPFVAAAEEEEEEEEEE----------DFMRALTFVRKRSRSCRKRQRIV 326

Query: 301 PIEAMRVIN 309
           P EA+RVIN
Sbjct: 352 PTEAVRVIN 326

BLAST of Cp4.1LG12g07100 vs. NCBI nr
Match: XP_022991785.1 (mitogen-activated protein kinase kinase kinase 17-like, partial [Cucurbita maxima])

HSP 1 Score: 485 bits (1249), Expect = 9.29e-171
Identity = 247/261 (94.64%), Postives = 250/261 (95.79%), Query Frame = 0

Query: 1   MASLKRSTAEFSIFPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRN 60
           MASLKRS A FSIFPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITT RN
Sbjct: 42  MASLKRSIAAFSIFPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITT-RN 101

Query: 61  GRMIYNLLLEIAVGGSLAHRINHTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKP 120
           GRMIYNLLLEIAVGGSLAHRIN+T GKGLAENEVGRYTKTIIKGLIHIH+RGYVHCDLKP
Sbjct: 102 GRMIYNLLLEIAVGGSLAHRINNTCGKGLAENEVGRYTKTIIKGLIHIHKRGYVHCDLKP 161

Query: 121 ANILLLPTNTAKGARLVAKICDLGLARRGSETRHGNCFGGTLSYMAPETLIHRVQESPSD 180
           ANILLLPTNTAKG RLVAKICDLGLARR  ETRH NCFGGTLSYMAPETLIHRVQESPSD
Sbjct: 162 ANILLLPTNTAKGGRLVAKICDLGLARRTRETRHDNCFGGTLSYMAPETLIHRVQESPSD 221

Query: 181 IWALGCLVLEMLTGNRPWAATDKAGIVKSITQNCGMPEIPEGVSKEASGFLKNCFVRNPE 240
           IWALGCLVLEMLTG  PWAATDKAGIVKSIT+N GMPEIPEGVSKEASGFLKNCFVRNPE
Sbjct: 222 IWALGCLVLEMLTGKCPWAATDKAGIVKSITENDGMPEIPEGVSKEASGFLKNCFVRNPE 281

Query: 241 YRFTAEMLMTVPFVAAAEEEE 261
           YRFTAEMLMTVPFVAAAEEEE
Sbjct: 282 YRFTAEMLMTVPFVAAAEEEE 301

BLAST of Cp4.1LG12g07100 vs. ExPASy TrEMBL
Match: A0A6J1GPL4 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=3662 GN=LOC111456326 PE=3 SV=1)

HSP 1 Score: 551 bits (1420), Expect = 2.02e-196
Identity = 286/309 (92.56%), Postives = 292/309 (94.50%), Query Frame = 0

Query: 1   MASLKRSTAEFSIFPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRN 60
           MASLKRS  EFSIFPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRN
Sbjct: 43  MASLKRS--EFSIFPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRN 102

Query: 61  GRMIYNLLLEIAVGGSLAHRINHTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKP 120
           GRMIYNL LEIA+GGSLAHRIN+ GGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKP
Sbjct: 103 GRMIYNLRLEIALGGSLAHRINNIGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKP 162

Query: 121 ANILLLPTNTAKGARLVAKICDLGLARRGSETRHGNCFGGTLSYMAPETLIHRVQESPSD 180
           ANILLLPTNTAKG RLVAKICDLGLARR SETRHGNCFGGTLSYMAPETLIHRVQESPSD
Sbjct: 163 ANILLLPTNTAKGGRLVAKICDLGLARRTSETRHGNCFGGTLSYMAPETLIHRVQESPSD 222

Query: 181 IWALGCLVLEMLTGNRPWAATDKAGIVKSITQNCGMPEIPEGVSKEASGFLKNCFVRNPE 240
           IWALGCLVLEMLTGNRPWAATD+AGIVKSIT+N GMPEIPEGVSK+ASGFLKNCFVRNPE
Sbjct: 223 IWALGCLVLEMLTGNRPWAATDEAGIVKSITENDGMPEIPEGVSKDASGFLKNCFVRNPE 282

Query: 241 YRFTAEMLMTVPFVAAAEEEEEEEEEEEEEEEEEEEEDFMRALTFVRKRSRSCRKRQRIV 300
           YRFTAEMLMTVPFVAAAEEEEEEEEEEE         DFMRALTFVRKRSRSCRKRQRIV
Sbjct: 283 YRFTAEMLMTVPFVAAAEEEEEEEEEEE---------DFMRALTFVRKRSRSCRKRQRIV 340

Query: 301 PIEAMRVIN 309
           P EA+RVIN
Sbjct: 343 PTEAVRVIN 340

BLAST of Cp4.1LG12g07100 vs. ExPASy TrEMBL
Match: A0A6J1JTX4 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=3661 GN=LOC111488322 PE=3 SV=1)

HSP 1 Score: 485 bits (1249), Expect = 4.50e-171
Identity = 247/261 (94.64%), Postives = 250/261 (95.79%), Query Frame = 0

Query: 1   MASLKRSTAEFSIFPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRN 60
           MASLKRS A FSIFPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITT RN
Sbjct: 42  MASLKRSIAAFSIFPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITT-RN 101

Query: 61  GRMIYNLLLEIAVGGSLAHRINHTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKP 120
           GRMIYNLLLEIAVGGSLAHRIN+T GKGLAENEVGRYTKTIIKGLIHIH+RGYVHCDLKP
Sbjct: 102 GRMIYNLLLEIAVGGSLAHRINNTCGKGLAENEVGRYTKTIIKGLIHIHKRGYVHCDLKP 161

Query: 121 ANILLLPTNTAKGARLVAKICDLGLARRGSETRHGNCFGGTLSYMAPETLIHRVQESPSD 180
           ANILLLPTNTAKG RLVAKICDLGLARR  ETRH NCFGGTLSYMAPETLIHRVQESPSD
Sbjct: 162 ANILLLPTNTAKGGRLVAKICDLGLARRTRETRHDNCFGGTLSYMAPETLIHRVQESPSD 221

Query: 181 IWALGCLVLEMLTGNRPWAATDKAGIVKSITQNCGMPEIPEGVSKEASGFLKNCFVRNPE 240
           IWALGCLVLEMLTG  PWAATDKAGIVKSIT+N GMPEIPEGVSKEASGFLKNCFVRNPE
Sbjct: 222 IWALGCLVLEMLTGKCPWAATDKAGIVKSITENDGMPEIPEGVSKEASGFLKNCFVRNPE 281

Query: 241 YRFTAEMLMTVPFVAAAEEEE 261
           YRFTAEMLMTVPFVAAAEEEE
Sbjct: 282 YRFTAEMLMTVPFVAAAEEEE 301

BLAST of Cp4.1LG12g07100 vs. ExPASy TrEMBL
Match: A0A1S3CFZ6 (mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103500025 PE=3 SV=1)

HSP 1 Score: 398 bits (1023), Expect = 3.61e-136
Identity = 212/309 (68.61%), Postives = 244/309 (78.96%), Query Frame = 0

Query: 1   MASLKRSTAEFSIFPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRN 60
           +A LK    ++SIFPPVMAVKSAE+SVS++LQKEKQ YDNLKGCNSLIQCFGEEITTD N
Sbjct: 43  LACLKPHFTKYSIFPPVMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHN 102

Query: 61  GRMIYNLLLEIAVGGSLAHRINHTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKP 120
           GRMIYNLLLE+A GG+LAH I +TGGKGL EN V  YTK+IIKGLIHIHR  YVHCDLKP
Sbjct: 103 GRMIYNLLLEVATGGTLAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKP 162

Query: 121 ANILLLP-TNTAKGARLVAKICDLGLARRGSETRHGNCFGGTLSYMAPETLIHRVQESPS 180
           ANILLLP  NT K  + +AKI DLGLARR S+T+   C GGT SYMAPETLI  VQES S
Sbjct: 163 ANILLLPKNNTTKDRQFIAKIADLGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESTS 222

Query: 181 DIWALGCLVLEMLTGNRPWAATDKAGIVKSITQN-CGMPEIPEGVSKEASGFLKNCFVRN 240
           DIWALGC+VLEMLTGNR WAATDK GI++ +T+N  GMP+IPEG+S EA+GFLKNC VR 
Sbjct: 223 DIWALGCVVLEMLTGNRAWAATDKVGILQEMTENFLGMPKIPEGLSAEATGFLKNCLVRK 282

Query: 241 PEYRFTAEMLMTVPFVAAAEEEEEEEEEEEEEEEEEEEEDF--MRALTFVRKRSRSCRKR 300
           PE+RFTAEML+ VPFVAAAE++E+               DF  ++A TFV K  R   KR
Sbjct: 283 PEFRFTAEMLINVPFVAAAEDQEQ---------------DFNTVKAPTFVTKWPRQF-KR 335

Query: 301 QRIVPIEAM 305
           QRI+PI+A+
Sbjct: 343 QRIIPIKAV 335

BLAST of Cp4.1LG12g07100 vs. ExPASy TrEMBL
Match: A0A0A0KBM3 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G430800 PE=3 SV=1)

HSP 1 Score: 397 bits (1021), Expect = 7.28e-136
Identity = 211/307 (68.73%), Postives = 240/307 (78.18%), Query Frame = 0

Query: 1   MASLKRSTAEFSIFPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRN 60
           +ASLK    ++SIFPPVMAVKSAE+SVS++LQKEKQ YDNLKGCNSLIQCFGEEITTD N
Sbjct: 43  LASLKPHFTKYSIFPPVMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHN 102

Query: 61  GRMIYNLLLEIAVGGSLAHRINHTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKP 120
           G MIYNLLLE+A GG+LAH I +TGGKGL EN V  YTK+IIKGLIHIHR  YVHCDLKP
Sbjct: 103 GHMIYNLLLEVATGGTLAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKP 162

Query: 121 ANILLLP-TNTAKGARLVAKICDLGLARRGSETRHGNCFGGTLSYMAPETLIHRVQESPS 180
           ANILLLP  NT K  + +AKI DLGLARR S+T+   C GGT SYMAPETLI  VQES S
Sbjct: 163 ANILLLPKNNTTKDRQFIAKIADLGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESAS 222

Query: 181 DIWALGCLVLEMLTGNRPWAATDKAGIVKSITQN-CGMPEIPEGVSKEASGFLKNCFVRN 240
           DIWALGC+VLEMLTGNR WAATDK GIVK +T+N  GMP+IPEG+S EA+GFLKNC VR 
Sbjct: 223 DIWALGCVVLEMLTGNRAWAATDKVGIVKEMTENFIGMPKIPEGLSPEATGFLKNCLVRK 282

Query: 241 PEYRFTAEMLMTVPFVAAAEEEEEEEEEEEEEEEEEEEEDFMRALTFVRKRSRSCRKRQR 300
           PE+RFTAEMLM VPFVAA E+ E++                ++A TFV K  R   KRQR
Sbjct: 283 PEFRFTAEMLMNVPFVAAGEDHEQDS-------------TTVKAPTFVTKWPRQF-KRQR 335

Query: 301 IVPIEAM 305
            +PI+A+
Sbjct: 343 TIPIKAV 335

BLAST of Cp4.1LG12g07100 vs. ExPASy TrEMBL
Match: A0A5D3CH22 (Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001440 PE=3 SV=1)

HSP 1 Score: 397 bits (1020), Expect = 1.03e-135
Identity = 212/309 (68.61%), Postives = 243/309 (78.64%), Query Frame = 0

Query: 1   MASLKRSTAEFSIFPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRN 60
           +A LK    ++SIFPPVMAVKSAE+SVS++LQKEKQ YDNLKGCNSLIQCFGEEITTD N
Sbjct: 43  LACLKPHFIKYSIFPPVMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHN 102

Query: 61  GRMIYNLLLEIAVGGSLAHRINHTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKP 120
           GRM YNLLLE+A GG+LAH I +TGGKGL EN V  YTK+IIKGLIHIHR  YVHCDLKP
Sbjct: 103 GRMTYNLLLEVATGGTLAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKP 162

Query: 121 ANILLLP-TNTAKGARLVAKICDLGLARRGSETRHGNCFGGTLSYMAPETLIHRVQESPS 180
           ANILLLP  NT K  + +AKI DLGLARR S+T+   C GGT SYMAPETLI  VQES S
Sbjct: 163 ANILLLPKNNTTKDRQFIAKIADLGLARRTSKTKGSYCLGGTFSYMAPETLIDGVQESTS 222

Query: 181 DIWALGCLVLEMLTGNRPWAATDKAGIVKSITQN-CGMPEIPEGVSKEASGFLKNCFVRN 240
           DIWALGC+VLEMLTGNR WAATDK GIV+ +T+N  GMP+IPEG+S EA+GFLKNC VR 
Sbjct: 223 DIWALGCVVLEMLTGNRAWAATDKVGIVQEMTENFLGMPKIPEGLSAEATGFLKNCLVRK 282

Query: 241 PEYRFTAEMLMTVPFVAAAEEEEEEEEEEEEEEEEEEEEDF--MRALTFVRKRSRSCRKR 300
           PE+RFTAEML+ VPFVAAAE++E+               DF  ++A TFV K  R   KR
Sbjct: 283 PEFRFTAEMLINVPFVAAAEDQEQ---------------DFNTVKAPTFVTKWPRQF-KR 335

Query: 301 QRIVPIEAM 305
           QRI+PI+A+
Sbjct: 343 QRIIPIKAV 335

BLAST of Cp4.1LG12g07100 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 193.0 bits (489), Expect = 3.7e-49
Identity = 113/260 (43.46%), Postives = 153/260 (58.85%), Query Frame = 0

Query: 14  FPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRNGRMIYNLLLEIAV 73
           FP +MAVKS+ V  S +L+ E+ + D+L  C+ +++CFGE  T + NG  IYNL LE A 
Sbjct: 30  FPSLMAVKSSGVVCSAALRNERDVLDDLGDCSEIVRCFGEGRTVE-NGEEIYNLFLEYAS 89

Query: 74  GGSLAHRINHTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKPANILLLPTNTAKG 133
           GGSLA RI  + G+ L E EV R+T++I+KGL HIH  G+ HCD+K  N+L+        
Sbjct: 90  GGSLADRIK-SSGEALPEFEVRRFTRSIVKGLCHIHGNGFTHCDIKLENVLVFGDGD--- 149

Query: 134 ARLVAKICDLGLARRGSETRHGNC---FGGTLSYMAPETLIHRVQESPSDIWALGCLVLE 193
                KI D GLA+R    R G       GT  YMAPE++ H   ESP+DIWALGC V+E
Sbjct: 150 ----VKISDFGLAKR----RSGEVCVEIRGTPLYMAPESVNHGEFESPADIWALGCSVVE 209

Query: 194 MLTGNRPWAATDKA-----GIVKSITQNCGMPEIPEGVSKEASGFLKNCFVRNPEYRFTA 253
           M +G   W   D        ++  I     +P IP  +S+E   F+  CFV+N   R+TA
Sbjct: 210 MSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVPRIPVELSEEGKDFVSKCFVKNAAERWTA 269

Query: 254 EMLMTVPFVAAAEEEEEEEE 266
           EML+  PF+A  +E  EE+E
Sbjct: 270 EMLLDHPFLAVDDESGEEDE 276

BLAST of Cp4.1LG12g07100 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 180.6 bits (457), Expect = 1.9e-45
Identity = 107/270 (39.63%), Postives = 157/270 (58.15%), Query Frame = 0

Query: 6   RSTAEFSIFPPVMAVKSAEVSVSKSLQKEKQIYDNL-KGCNSLIQCFGEEITTDRNGRMI 65
           RS  +   FPP+MAVKSA+   + SL  EK + DNL   CN +++CFGE+ T + NG  +
Sbjct: 22  RSNNDSGEFPPLMAVKSADSYGAASLANEKSVLDNLGDDCNEIVRCFGEDRTVE-NGEEM 81

Query: 66  YNLLLEIAVGGSLAHRINHTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKPANIL 125
           +NL LE A  GSL   +    G+G+ E+ V R+T ++++GL HIH  G+ HCDLK  NIL
Sbjct: 82  HNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSVLRGLRHIHANGFAHCDLKLGNIL 141

Query: 126 LLPTNTAKGARLVAKICDLGLARRGSE---TRHGNCFGGTLSYMAPETLIHRVQESPSDI 185
           L       GA    KI D GLA+R  +     +G    GT  YMAPE++      S  D+
Sbjct: 142 LF----GDGA---VKIADFGLAKRIGDLTALNYGVQIRGTPLYMAPESVNDNEYGSEGDV 201

Query: 186 WALGCLVLEMLTGNRPWAATDKAGIVKSITQ---NCGMPEIPEGVSKEASGFLKNCFVRN 245
           WALGC+V+EM +G   W+  + +  +  + +      +P IPE +S++   FL  CFV++
Sbjct: 202 WALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDEVPMIPEELSEQGRDFLSKCFVKD 261

Query: 246 PEYRFTAEMLMTVPFVAAAEEEEEEEEEEE 269
           P+ R+TAEML+  PFV    + +   +EE+
Sbjct: 262 PKKRWTAEMLLNHPFVTVDVDHDVLVKEED 283

BLAST of Cp4.1LG12g07100 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 170.6 bits (431), Expect = 2.0e-42
Identity = 106/269 (39.41%), Postives = 156/269 (57.99%), Query Frame = 0

Query: 14  FPPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITTDRNGRMIYNLLLEIAV 73
           FP ++AVKS +   + SL  EK + D+L  C  +I+C+GE+ T + NG  ++NLLLE A 
Sbjct: 29  FPALIAVKSTDAYGAASLSNEKSVLDSLGDCPEIIRCYGEDSTVE-NGEEMHNLLLEYAS 88

Query: 74  GGSLAHRINHTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKPANILLLPTNTAKG 133
            GSLA  +   GG+GL E+ V R+T ++++GL HIH +G+ HCD+K ANILL    +   
Sbjct: 89  RGSLASYMKKLGGEGLPESTVRRHTGSVLRGLRHIHAKGFAHCDIKLANILLFNDGS--- 148

Query: 134 ARLVAKICDLGLARR--GSET--RHGNCFGGTLSYMAPETLIHRVQESPSDIWALGCLVL 193
                KI D GLA R  G  T  R      GT  YMAPE +      S +D+WALGC V+
Sbjct: 149 ----VKIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPECVNDNEYGSAADVWALGCAVV 208

Query: 194 EMLTGNRPWAATDKA---GIVKSITQNCGMPEIPEGVSKEASGFLKNCFVRNPEYRFTAE 253
           EM +G   W+  + +    ++  I     +P+IPE +S+E   FL  CFV++P  R+TAE
Sbjct: 209 EMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSEEGKDFLSKCFVKDPAKRWTAE 268

Query: 254 MLMTVPFVAAAEEEEEEEEEEEEEEEEEE 276
           ML+   FV    E++  E    + ++E++
Sbjct: 269 MLLNHSFVTIDLEDDHRENFVVKVKDEDK 289

BLAST of Cp4.1LG12g07100 vs. TAIR 10
Match: AT5G27510.1 (Protein kinase superfamily protein )

HSP 1 Score: 165.6 bits (418), Expect = 6.3e-41
Identity = 99/242 (40.91%), Postives = 133/242 (54.96%), Query Frame = 0

Query: 19  AVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGEEITT--DRNGRMIYNLLLEIAVGGS 78
           AVKS++     SL KE  I   LKGC  +IQCFG ++    D  G  +Y LLLE A  GS
Sbjct: 35  AVKSSD--DENSLLKEFHILSELKGCPRIIQCFGNDLEEGFDDKGNRVYKLLLEYASEGS 94

Query: 79  LAHRINHTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKPANILLLPTNTAKGARL 138
           L+  +N+   + L +  +  +T+ I++GL+ IH  GYVHCDLKP N+L+ P     G   
Sbjct: 95  LSDFMNNCVDRKLPDLMIRDFTRMILQGLVSIHSHGYVHCDLKPENVLVFPC----GDSY 154

Query: 139 VAKICDLGLARR-GSETRHGNC---FGGTLSYMAPETLIHRVQESPSDIWALGCLVLEML 198
             KI D GL+ + G    H      F GTL+YM PE+L   V     D+W+LGCLVLEM 
Sbjct: 155 EVKISDFGLSLQVGEVPDHWKIEYPFVGTLNYMPPESLHDGVANKTLDLWSLGCLVLEMY 214

Query: 199 TGNRPWAATDKAGIVKSITQNCGMPEIPEGVSKEASGFLKNCFVRNPEYRFTAEMLMTVP 255
              +PW        V  I  N   PEIPE +  +A  F++ CF RNP+ R TA  L++  
Sbjct: 215 VCKKPWIGFIPEDFV-YILSNGNPPEIPESLPCDARAFIQKCFSRNPKERGTASELLSHR 269

BLAST of Cp4.1LG12g07100 vs. TAIR 10
Match: AT2G42550.1 (Protein kinase superfamily protein )

HSP 1 Score: 163.7 bits (413), Expect = 2.4e-40
Identity = 91/249 (36.55%), Postives = 141/249 (56.63%), Query Frame = 0

Query: 15  PPVMAVKSAEVSVSKSLQKEKQIYDNLKGCNSLIQCFGE---EITTDRNGRMIYNLLLEI 74
           P   AVK+AE     SL++E QI   L+GC  ++QC+G    E   D  G  +Y +++E 
Sbjct: 33  PLYAAVKTAECEDYNSLEREIQILSKLEGCRRIVQCYGNYTLEEDFDVGGFRVYKMVMEY 92

Query: 75  AVGGSLAHRINHTGGKGLAENEVGRYTKTIIKGLIHIHRRGYVHCDLKPANILLLPTNTA 134
           A  GSL   ++    + L E  +  +T+ I++GL+ +HR GYVHCDLKP N+L+ P   +
Sbjct: 93  AAAGSLFSFMDSYKDRKLPETMIKDFTRMILQGLVSVHRLGYVHCDLKPDNLLVFPCRQS 152

Query: 135 KGARLVAKICDLGLARRGSETRHGNC------FGGTLSYMAPETLIHRVQESPSDIWALG 194
                  KI D G +R+  E  + +C      F GT  YM+PE++   V E   D+W+LG
Sbjct: 153 ----YELKISDFGSSRKVGE--YSDCWDVDLPFVGTPVYMSPESVRSGVAEKALDLWSLG 212

Query: 195 CLVLEMLTGNRPWAATDKAGIVKSITQNCGMPEIPEGVSKEASGFLKNCFVRNPEYRFTA 254
           C+VLEM TG  PW+  +   +  ++++    PEIP+ +  +A  FL+ CF RNP+ R +A
Sbjct: 213 CIVLEMYTGVIPWSEVEFEDLAPALSKG-KAPEIPKSLPCDARKFLETCFSRNPKERGSA 272

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SND62.7e-4139.41Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370... [more]
O808881.2e-3135.15Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370... [more]
P288294.3e-2633.86Protein kinase byr2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=28... [more]
Q9C5H55.6e-2633.60Mitogen-activated protein kinase kinase kinase 5 OS=Arabidopsis thaliana OX=3702... [more]
Q013893.1e-2432.55Serine/threonine-protein kinase BCK1/SLK1/SSP31 OS=Saccharomyces cerevisiae (str... [more]
Match NameE-valueIdentityDescription
KAG7014197.12.41e-20295.15Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosper... [more]
XP_022953922.14.18e-19692.56mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata][more]
XP_023548452.11.04e-174100.00mitogen-activated protein kinase kinase kinase 17-like, partial [Cucurbita pepo ... [more]
KAG6575645.12.48e-17385.44Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosper... [more]
XP_022991785.19.29e-17194.64mitogen-activated protein kinase kinase kinase 17-like, partial [Cucurbita maxim... [more]
Match NameE-valueIdentityDescription
A0A6J1GPL42.02e-19692.56mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=... [more]
A0A6J1JTX44.50e-17194.64mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=36... [more]
A0A1S3CFZ63.61e-13668.61mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN... [more]
A0A0A0KBM37.28e-13668.73Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G430... [more]
A0A5D3CH221.03e-13568.61Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuw... [more]
Match NameE-valueIdentityDescription
AT4G36950.13.7e-4943.46mitogen-activated protein kinase kinase kinase 21 [more]
AT5G67080.11.9e-4539.63mitogen-activated protein kinase kinase kinase 19 [more]
AT3G50310.12.0e-4239.41mitogen-activated protein kinase kinase kinase 20 [more]
AT5G27510.16.3e-4140.91Protein kinase superfamily protein [more]
AT2G42550.12.4e-4036.55Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 253..283
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 16..309
e-value: 6.0E-52
score: 178.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 256..279
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 256..280
NoneNo IPR availablePANTHERPTHR48011:SF56MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 3-LIKEcoord: 9..277
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 9..277
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 8..254
e-value: 6.7E-35
score: 131.9
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 18..253
e-value: 1.2E-42
score: 146.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 1..254
score: 32.696903
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 113..125
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 25..257

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG12g07100.1Cp4.1LG12g07100.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity