Cp4.1LG12g06870 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG12g06870
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionSiroheme synthase
LocationCp4.1LG12: 7256563 .. 7259938 (-)
RNA-Seq ExpressionCp4.1LG12g06870
SyntenyCp4.1LG12g06870
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGGCTATACTTTCCGCGAGATGATCGTGAGCTCATTTACTTGATAATAATCATGGGCCAGCCCATTATAACGGATAGGATTACTGGACCACGAGGCCCAATTCAGCTACGGCCCAATTCAGCTACGGCCCATATAACGAAGAGAGTTGCGCATTTATTTGCTATTTCAAAAGAAAAAGGCAGAAAAAATCCTCAAAGTTTCCAATTCAGCTGTGAAATTCATGGCAGCTGATAGACAGTCCCAGGGATTTCAATTTGGTCTCTCGCACATCTCAAAAGGCCACTCCTTGTCTCCTTCCCCTTTTCCTTCATTTTTTTTTAGGCAGGCCGCCGCCACTCCTTTCCTTTTGCCTTCTCATCTTCCTCAAAGTCCATGTGTTAGAGCCGTAGAGCCACAGGCTTCCTCTCATTTATCCCATATTTATTCCCCTTCCGGACATCGCTTTTTCCCACTTTCTCCTTCGCCATGGCTCGTGTTTGCGACCTACAGTCTCTTTCATCGCTATTTGCGTCTCGCCCAACAATACCCAGATCCTCAAATTTCAAACCCATTTGTTCTTTTCACTGCTCTTCTTCTTCTTCGCCATTTACCGAGAAACATTCCGTGAAGAGATACCAAAGAGACGATTGGCTGTACAAGAACCAAGCGGATGAAAGTTCTGTTGCTTCATCGTGTTCTATTCCGTCTGATTCTGAGTCTATACGGCAGAATGACATTGCCTTGCAGCTGCCGGAGCTGAAGAAATTGCTGCAGGTGCTGAGGGAAAAGAGGGCAAGTAGTGGTTGCGATGATGGGAAATGTGGGCCGGGGAATGTGTTTTTGGTGGGGACTGGCCCTGGAGATCCTGAGCTTTTGACATTGAAGGCGGTGAAAGTGATTCAGAGTGCTGATTTGCTTTTGTATGATCGATTGGTTTCTAATGATGTGTTGGATTTGGTGGGTTCTGATGCTAGGCTTCTCTATGTGGGGAAGACGGCTGGTTTCCATAGCAGAACCCAGGTGAGCTTTTTCTTCTGAAAATCTCTACTTAATATGGGTTTGGTTTGGCTTTTTTTGAGATTGTTGCTGAATGGGTATTTGTTTAGGAGGAGATTCATGAGTTACTTCTGAACTTTGCTGAAGCTGGAGCTACAGTTGTGAGGCTTAAAGGAGGAGACCCTCTTGTAAGTGTGCATTTCGAATGGCTCATTCAAATGATTTTTAGTGATGCATCCTGAAAATTCTGTTGTTTGTATTCCTTTTAGGTGTTTGGAAGGGGTGGGGAGGAGATGGATTTCTTGCAACAGCAAGGGATTCAAGTCAAAATTGTTCCTGGTAAGTGCATTTCTGAATTCTGTTCTTCAGTCCCTTCAGAAGTGGATGGACCATATTAGGTTAGAATGTAACACCTGTTTGGGATGGCTAAAGTCGTAGGGAAGGCACTCGTAGGTCCTTGCTCCCAGCGAGGCTTACGTTCTAACACATCGACGTAGAGCATTTCTATGAGATCCCACATTGGTGGGGAGGGGAACGAAGCATTCTTTATAAGGACGTGGAAATCTCTCCCTAGCATATACGTTTATAACCTTGAGGGGGAGCTCAAAAGGGAAAGCCTAAAGAGAATAACATCGGTTAGCGGTACCTATTTTCTGTTCTTGCTCCTTCCAAACGAAGAGAATGGAGTGATATTAAGTTATGTGGAATAGAACCAGGAGTAAGAAATTAGGTAAGATGGGCTCGAGTACCTGAGGACGATTTGGTTACAAGGTCTCTTGAATTCGCTTCGTGCTCGTAATCAATCATTGAACTGTCATTAACATATCACATTCTGATTGCCAATGCACCACCAGTTTAAGGAAACTACTCTTAAGAGTAACCTTTGTTTCCCTTGCAGGGATAACTGCGGCTTCGGGTATAGCTGCTGAATTGGGAATTCCTTTGACACACAGGGGCGTTGCGACAAGTGTCAGGTTCCTCACTGGTCACTCGAGACAGGGTGGAACGGATCCTCTATTTGTGGCAGAGAATGCAGCTGATCCAGATTCAACTCTGGTGGTATACATGGGTCTATCAACTCTTCCATCTCTGGCCCTTAAGTTGATGCATCATGGTCTGCCCCCTGATACCCCAGCTGCTGCCATTGAACGAGGGACAACACCCCAACAAAGAATAGTAAGCACAATTCTCTGCTGCCTTCAACTTTTGTTCTGAGTTGGGTAGGGTTCAACCAAACACTCTTAACGTGTCGGTTCGTTACCATTCTAGCAAATCTACTTAAACCAGGAGATCTCTCTGCGTTGAGCTACTTTTTCAGTGACATCTGGATCTAGACTTTAATATCAAGGGGGCAGGGCATACTTTCACTTAAGTCTTTTCATTTCATCTTGGTCGTGCCTTGAACAAATCTTGCTTGGATCTATTCCATTTGTGCAGGTTTTTGCAGAACTGAAGGATCTTGCAGATGAAATCAAAGCAGCAGAGTTGGTTTCACCTACTTTGATTATAATTGGAAAAGTGGTTTCTCTCTCACCACATTGGTCACTTTCCTCCAAAGAAGCCTCCAGTTTGGTGGAGGCTTAACTGAGTTAAAAGTGTCTACAAAAGGTTACAGGAATAGTTCTTCATTCTGTCGCTTAAAAAGCTCTGGACGTCGAGTTTTTCCACAAGATCTTGTCGATTTTGTGGAGAAAAACATTGAAACCATGACAGAGCAGAAAAAGAAGAATAGGGGACTCGTCTTTCTGTTCTGCATTTTATTGGTCTGCCCATTGCCTTCAAATGGCTGGACTCGGGTTTTCTGCGAAGGACGGGAGTGATGCAGTGGTTTTCCTCCTTGGATGCTACTACCGGAGCACGGTGAGCAGCATCTCCTCATACCATTTCTTGCATTGATTGAAATAGAACCTCCAAACTTCAATTTTTTCTTAGTTCATGTTCATGAGGCCTCTGGTGCAATGAGATTACTTTATCACCAACCAATGTATTGTAGAATTGTCTTTTATCTTGAGTAGAACTTCTACAAATAACAAAGAAATACAGACTACGAACGGCAAGCTCGTAAATATTGTAGAACTTCACCTCAAATATGACCTCATAGTTCCTGCAGTCTGGATTTGTGAATAATAAGGTTTCACTCCAAATGTCATTAAAAAAAACATATATACGATATTCTAATCACTACTCCATAATCTCTCCTTCCTCCAGTTCTTCAACTCCCTTGGAAAAGTCTCCTTCCCAATCAAGAAAATCATCTGGATTGATTTGTGCCATGATATTTGAGTAAGTATCTATCAGATCTTGCTCAAAATCAAACTCCAATCCTTTCGAAACCGCTATCTCCTCGGCCTGGGAGCTTGAATTCGGGCCATTCCCAAACTCATGCTTCCCCATCAAATCT

mRNA sequence

CGGCTATACTTTCCGCGAGATGATCGTGAGCTCATTTACTTGATAATAATCATGGGCCAGCCCATTATAACGGATAGGATTACTGGACCACGAGGCCCAATTCAGCTACGGCCCAATTCAGCTACGGCCCATATAACGAAGAGAGTTGCGCATTTATTTGCTATTTCAAAAGAAAAAGGCAGAAAAAATCCTCAAAGTTTCCAATTCAGCTGTGAAATTCATGGCAGCTGATAGACAGTCCCAGGGATTTCAATTTGGTCTCTCGCACATCTCAAAAGGCCACTCCTTGTCTCCTTCCCCTTTTCCTTCATTTTTTTTTAGGCAGGCCGCCGCCACTCCTTTCCTTTTGCCTTCTCATCTTCCTCAAAGTCCATGTGTTAGAGCCGTAGAGCCACAGGCTTCCTCTCATTTATCCCATATTTATTCCCCTTCCGGACATCGCTTTTTCCCACTTTCTCCTTCGCCATGGCTCGTGTTTGCGACCTACAGTCTCTTTCATCGCTATTTGCGTCTCGCCCAACAATACCCAGATCCTCAAATTTCAAACCCATTTGTTCTTTTCACTGCTCTTCTTCTTCTTCGCCATTTACCGAGAAACATTCCGTGAAGAGATACCAAAGAGACGATTGGCTGTACAAGAACCAAGCGGATGAAAGTTCTGTTGCTTCATCGTGTTCTATTCCGTCTGATTCTGAGTCTATACGGCAGAATGACATTGCCTTGCAGCTGCCGGAGCTGAAGAAATTGCTGCAGGTGCTGAGGGAAAAGAGGGCAAGTAGTGGTTGCGATGATGGGAAATGTGGGCCGGGGAATGTGTTTTTGGTGGGGACTGGCCCTGGAGATCCTGAGCTTTTGACATTGAAGGCGGTGAAAGTGATTCAGAGTGCTGATTTGCTTTTGTATGATCGATTGGTTTCTAATGATGTGTTGGATTTGGTGGGTTCTGATGCTAGGCTTCTCTATGTGGGGAAGACGGCTGGTTTCCATAGCAGAACCCAGGAGGAGATTCATGAGTTACTTCTGAACTTTGCTGAAGCTGGAGCTACAGTTGTGAGGCTTAAAGGAGGAGACCCTCTTGTGTTTGGAAGGGGTGGGGAGGAGATGGATTTCTTGCAACAGCAAGGGATTCAAGTCAAAATTGTTCCTGGGATAACTGCGGCTTCGGGTATAGCTGCTGAATTGGGAATTCCTTTGACACACAGGGGCGTTGCGACAAGTGTCAGGTTCCTCACTGGTCACTCGAGACAGGGTGGAACGGATCCTCTATTTGTGGCAGAGAATGCAGCTGATCCAGATTCAACTCTGGTGGTATACATGGGTCTATCAACTCTTCCATCTCTGGCCCTTAAGTTGATGCATCATGGTCTGCCCCCTGATACCCCAGCTGCTGCCATTGAACGAGGGACAACACCCCAACAAAGAATAGTTTTTGCAGAACTGAAGGATCTTGCAGATGAAATCAAAGCAGCAGAGTTGGTTTCACCTACTTTGATTATAATTGGAAAAGTGGTTTCTCTCTCACCACATTGGTCACTTTCCTCCAAAGAAGCCTCCAGTTTGGTGGAGGCTTAACTGAGTTAAAAGTGTCTACAAAAGGTTACAGGAATAGTTCTTCATTCTGTCGCTTAAAAAGCTCTGGACGTCGAGTTTTTCCACAAGATCTTGTCGATTTTGTGGAGAAAAACATTGAAACCATGACAGAGCAGAAAAAGAAGAATAGGGGACTCGTCTTTCTGTTCTGCATTTTATTGGTCTGCCCATTGCCTTCAAATGGCTGGACTCGGGTTTTCTGCGAAGGACGGGAGTGATGCAGTGGTTTTCCTCCTTGGATGCTACTACCGGAGCACGGTGAGCAGCATCTCCTCATACCATTTCTTGCATTGATTGAAATAGAACCTCCAAACTTCAATTTTTTCTTAGTTCATGTTCATGAGGCCTCTGGTGCAATGAGATTACTTTATCACCAACCAATGTATTGTAGAATTGTCTTTTATCTTGAGTAGAACTTCTACAAATAACAAAGAAATACAGACTACGAACGGCAAGCTCGTAAATATTGTAGAACTTCACCTCAAATATGACCTCATAGTTCCTGCAGTCTGGATTTGTGAATAATAAGGTTTCACTCCAAATGTCATTAAAAAAAACATATATACGATATTCTAATCACTACTCCATAATCTCTCCTTCCTCCAGTTCTTCAACTCCCTTGGAAAAGTCTCCTTCCCAATCAAGAAAATCATCTGGATTGATTTGTGCCATGATATTTGAGTAAGTATCTATCAGATCTTGCTCAAAATCAAACTCCAATCCTTTCGAAACCGCTATCTCCTCGGCCTGGGAGCTTGAATTCGGGCCATTCCCAAACTCATGCTTCCCCATCAAATCT

Coding sequence (CDS)

ATGGCTCGTGTTTGCGACCTACAGTCTCTTTCATCGCTATTTGCGTCTCGCCCAACAATACCCAGATCCTCAAATTTCAAACCCATTTGTTCTTTTCACTGCTCTTCTTCTTCTTCGCCATTTACCGAGAAACATTCCGTGAAGAGATACCAAAGAGACGATTGGCTGTACAAGAACCAAGCGGATGAAAGTTCTGTTGCTTCATCGTGTTCTATTCCGTCTGATTCTGAGTCTATACGGCAGAATGACATTGCCTTGCAGCTGCCGGAGCTGAAGAAATTGCTGCAGGTGCTGAGGGAAAAGAGGGCAAGTAGTGGTTGCGATGATGGGAAATGTGGGCCGGGGAATGTGTTTTTGGTGGGGACTGGCCCTGGAGATCCTGAGCTTTTGACATTGAAGGCGGTGAAAGTGATTCAGAGTGCTGATTTGCTTTTGTATGATCGATTGGTTTCTAATGATGTGTTGGATTTGGTGGGTTCTGATGCTAGGCTTCTCTATGTGGGGAAGACGGCTGGTTTCCATAGCAGAACCCAGGAGGAGATTCATGAGTTACTTCTGAACTTTGCTGAAGCTGGAGCTACAGTTGTGAGGCTTAAAGGAGGAGACCCTCTTGTGTTTGGAAGGGGTGGGGAGGAGATGGATTTCTTGCAACAGCAAGGGATTCAAGTCAAAATTGTTCCTGGGATAACTGCGGCTTCGGGTATAGCTGCTGAATTGGGAATTCCTTTGACACACAGGGGCGTTGCGACAAGTGTCAGGTTCCTCACTGGTCACTCGAGACAGGGTGGAACGGATCCTCTATTTGTGGCAGAGAATGCAGCTGATCCAGATTCAACTCTGGTGGTATACATGGGTCTATCAACTCTTCCATCTCTGGCCCTTAAGTTGATGCATCATGGTCTGCCCCCTGATACCCCAGCTGCTGCCATTGAACGAGGGACAACACCCCAACAAAGAATAGTTTTTGCAGAACTGAAGGATCTTGCAGATGAAATCAAAGCAGCAGAGTTGGTTTCACCTACTTTGATTATAATTGGAAAAGTGGTTTCTCTCTCACCACATTGGTCACTTTCCTCCAAAGAAGCCTCCAGTTTGGTGGAGGCTTAA

Protein sequence

MARVCDLQSLSSLFASRPTIPRSSNFKPICSFHCSSSSSPFTEKHSVKRYQRDDWLYKNQADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSGCDDGKCGPGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAAELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSKEASSLVEA
Homology
BLAST of Cp4.1LG12g06870 vs. ExPASy Swiss-Prot
Match: Q42606 (S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=UPM1 PE=1 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 4.4e-147
Identity = 274/347 (78.96%), Postives = 295/347 (85.01%), Query Frame = 0

Query: 24  SNFKPICSFH---CSSSSSPFTEKHSVKRYQRDDWLYKNQADESSVASSCSIPSDSESIR 83
           +N  PIC  H    SSSSSPFTEKHSV+RYQRD WLYK          S S   D   +R
Sbjct: 22  TNLTPICCLHYNTASSSSSPFTEKHSVERYQRDQWLYKAVEPTPPSTPSPSPFEDEVFVR 81

Query: 84  QNDIALQLPELKKLLQVLREKRASSGCDDGKCGPGNVFLVGTGPGDPELLTLKAVKVIQS 143
           +NDIA QLPELKKLL VL+EKR   GC  G CGPG+V+LVGTGPGDPELLTLKAV+VIQS
Sbjct: 82  ENDIASQLPELKKLLAVLKEKRV-KGCKGGDCGPGDVYLVGTGPGDPELLTLKAVRVIQS 141

Query: 144 ADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRTQEEIHELLLNFAEAGATVVRLKG 203
           ADLLLYDRLVSNDVL+LV  DARLLYVGKTAG+HSRTQEEIHELLLNFAEAGATVVRLKG
Sbjct: 142 ADLLLYDRLVSNDVLELVAPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKG 201

Query: 204 GDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAAELGIPLTHRGVATSVRFLTGHSR 263
           GDPLVFGRGGEEMDFLQQQGI+V+++PGITAASGIAAELGIPLTHRGVATSVRFLTGHSR
Sbjct: 202 GDPLVFGRGGEEMDFLQQQGIRVQVIPGITAASGIAAELGIPLTHRGVATSVRFLTGHSR 261

Query: 264 QGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHHGLPPDTPAAAIERGTTPQQRI 323
           +GGTDPLFVAENAADPD+TLVVYMGL TLPSLA KLM HGLP DTPA A+ERGTTP QR 
Sbjct: 262 KGGTDPLFVAENAADPDTTLVVYMGLGTLPSLAQKLMDHGLPSDTPAVAVERGTTPLQRT 321

Query: 324 VFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSKEASSLVE 368
           VFAELKD A EI++A LVSPTLIIIGKVV LSP W   +KE+S LVE
Sbjct: 322 VFAELKDFATEIQSAGLVSPTLIIIGKVVELSPLWPHCTKESSCLVE 367

BLAST of Cp4.1LG12g06870 vs. ExPASy Swiss-Prot
Match: B8GUD3 (Siroheme synthase OS=Thioalkalivibrio sulfidiphilus (strain HL-EbGR7) OX=396588 GN=cysG PE=3 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 5.1e-63
Identity = 125/252 (49.60%), Postives = 170/252 (67.46%), Query Frame = 0

Query: 100 EKRASSGCDDGKCGPGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVG 159
           EK   +G D    G G VFLVG GPGDP+LLT +A++++Q AD+++YD LVS  +++LV 
Sbjct: 204 EKALETGLDTRDAG-GEVFLVGAGPGDPDLLTFRALRLMQLADVVVYDNLVSPAIIELVR 263

Query: 160 SDARLLYVGKTAGFHSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQ 219
            DA ++Y GK    H+  QEEI++LL+  A+ G  V+RLKGGDP +FGRGGEE+D L Q+
Sbjct: 264 RDAEMIYAGKKRNLHTLPQEEINQLLVRLAKEGKRVLRLKGGDPFIFGRGGEEIDTLMQE 323

Query: 220 GIQVKIVPGITAASGIAAELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDST 279
           GI  ++VPGITAA+G A+  GIPLTHR  A +V F TGH R G  D  +  +  A P  T
Sbjct: 324 GIPFQVVPGITAAAGCASFSGIPLTHRDYAQAVVFATGHLRDGSIDLNW--KMLAQPRQT 383

Query: 280 LVVYMGLSTLPSLALKLMHHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVS 339
           +V YMGL  LP +  +LM HG+ PD P A +E+GTT  QR++   L  + D +K  ++  
Sbjct: 384 VVFYMGLLGLPIICRELMAHGVSPDMPMALVEQGTTQNQRVIVGTLASMPDLVKDYDVQP 443

Query: 340 PTLIIIGKVVSL 352
           PTLII+G+VV L
Sbjct: 444 PTLIIVGEVVKL 452

BLAST of Cp4.1LG12g06870 vs. ExPASy Swiss-Prot
Match: A0KP37 (Siroheme synthase 2 OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / BCRC 13018 / CCUG 14551 / JCM 1027 / KCTC 2358 / NCIMB 9240 / NCTC 8049) OX=380703 GN=cysG2 PE=3 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 4.3e-62
Identity = 132/252 (52.38%), Postives = 172/252 (68.25%), Query Frame = 0

Query: 100 EKRASSGCDDGKCGPGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVG 159
           E+  + G D  K   G V LVG GPGDP LLTLKA++ IQ A+++LYD+LVS ++LDLV 
Sbjct: 199 EQWLNDGLDQAKNEVGEVVLVGAGPGDPGLLTLKALQQIQQAEVVLYDQLVSPEILDLVR 258

Query: 160 SDARLLYVGKTAGFHSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQ 219
            DA L+ VGK AG HS  QEE + LL+ +A+AG  VVRLKGGDP +FGRGGEE++ L ++
Sbjct: 259 RDATLVSVGKKAGAHSVPQEETNRLLVEYAKAGNRVVRLKGGDPFMFGRGGEELEVLAEE 318

Query: 220 GIQVKIVPGITAASGIAAELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDST 279
           GI   +VPGITAA+G  A  GIPLTHR  A S  F+TGH +  G +P +  +  A    T
Sbjct: 319 GIPFSVVPGITAAAGATAYAGIPLTHRDHAQSAVFITGHCQIDGKEPDW--QQLAATSQT 378

Query: 280 LVVYMGLSTLPSLALKLMHHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVS 339
           LV+YMGL     +  +L+ HG    TP A IERGTT +QR++   L DLA+   AA+ VS
Sbjct: 379 LVIYMGLMRSEHIQQQLVSHGRSSATPIAIIERGTTARQRVLTGTLADLAE--LAAQAVS 438

Query: 340 PTLIIIGKVVSL 352
           P+LI+IG+VV+L
Sbjct: 439 PSLIVIGEVVAL 446

BLAST of Cp4.1LG12g06870 vs. ExPASy Swiss-Prot
Match: Q0VQ05 (Siroheme synthase OS=Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / NCIMB 13689 / SK2) OX=393595 GN=cysG PE=3 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 1.3e-61
Identity = 120/239 (50.21%), Postives = 162/239 (67.78%), Query Frame = 0

Query: 115 GNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFH 174
           G V+LVG GPGDP+LLT +A++++Q AD++LYDRLV   ++DL   DA L+YVGK    H
Sbjct: 218 GEVYLVGAGPGDPDLLTFRALRLLQKADVVLYDRLVGKGIVDLARRDAELVYVGKARDKH 277

Query: 175 SRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASG 234
           +  Q+ I+ELL+++A+ G  V RLKGGDP +FGRGGEE+D +  +GI  ++VPGITAASG
Sbjct: 278 ALPQDNINELLVHYAKQGKKVCRLKGGDPFIFGRGGEEIDLIVAEGIDFQVVPGITAASG 337

Query: 235 IAAELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLAL 294
            A+  GIPLTHR  A SVRF+TGH + G  D     ++      T+V YMGL  L  +  
Sbjct: 338 CASYAGIPLTHRDHAQSVRFVTGHRKDGSVD--LDWKHLVSETETVVFYMGLVGLREICS 397

Query: 295 KLMHHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSP 354
           +L+ HG   DTP A + RGTT  Q ++   L  L D+I+  E+ +PTLII+G VVSL P
Sbjct: 398 QLIAHGRGGDTPIALVSRGTTNLQEVITGRLDQLPDDIEGREIHAPTLIIVGSVVSLHP 454

BLAST of Cp4.1LG12g06870 vs. ExPASy Swiss-Prot
Match: Q6F8G6 (Siroheme synthase OS=Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) OX=62977 GN=cysG PE=3 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 2.2e-61
Identity = 124/237 (52.32%), Postives = 163/237 (68.78%), Query Frame = 0

Query: 115 GNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFH 174
           G V+LVG GPGDPELLTLKA++++Q AD+++YDRLVS  +L+L   DA  +YVGK    H
Sbjct: 216 GEVYLVGAGPGDPELLTLKALRLMQQADVVIYDRLVSAPILELCRRDAEKVYVGKARSNH 275

Query: 175 SRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASG 234
           S  QE I+ LL+ +A+AG  V RLKGGDP +FGRGGEE+  L   GI  ++VPGITAASG
Sbjct: 276 SVPQEGINALLVKYAQAGKRVCRLKGGDPFIFGRGGEEIQELFAAGIPFQVVPGITAASG 335

Query: 235 IAAELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLAL 294
            +A  GIPLTHR  A SVRFLTGH ++G   P          + TLV+YMGL  L  +  
Sbjct: 336 CSAYAGIPLTHRDYAQSVRFLTGHLKEG--SPELPWSELVYENQTLVLYMGLVGLEHICQ 395

Query: 295 KLMHHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSL 352
           +L+ HG  PD P A + +GTTP+Q++V   L ++A +I   ++ +PTL IIG+VVSL
Sbjct: 396 QLIAHGQRPDMPVALVSKGTTPEQKVVVGTLSNIASKIAEYQIHAPTLTIIGEVVSL 450

BLAST of Cp4.1LG12g06870 vs. NCBI nr
Match: XP_023548332.1 (uncharacterized protein LOC111807000 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 716 bits (1847), Expect = 8.02e-260
Identity = 368/368 (100.00%), Postives = 368/368 (100.00%), Query Frame = 0

Query: 1   MARVCDLQSLSSLFASRPTIPRSSNFKPICSFHCSSSSSPFTEKHSVKRYQRDDWLYKNQ 60
           MARVCDLQSLSSLFASRPTIPRSSNFKPICSFHCSSSSSPFTEKHSVKRYQRDDWLYKNQ
Sbjct: 1   MARVCDLQSLSSLFASRPTIPRSSNFKPICSFHCSSSSSPFTEKHSVKRYQRDDWLYKNQ 60

Query: 61  ADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSGCDDGKCGPGNVFLV 120
           ADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSGCDDGKCGPGNVFLV
Sbjct: 61  ADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSGCDDGKCGPGNVFLV 120

Query: 121 GTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRTQEE 180
           GTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRTQEE
Sbjct: 121 GTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRTQEE 180

Query: 181 IHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAAELG 240
           IHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAAELG
Sbjct: 181 IHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAAELG 240

Query: 241 IPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHHG 300
           IPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHHG
Sbjct: 241 IPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHHG 300

Query: 301 LPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSK 360
           LPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSK
Sbjct: 301 LPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSK 360

Query: 361 EASSLVEA 368
           EASSLVEA
Sbjct: 361 EASSLVEA 368

BLAST of Cp4.1LG12g06870 vs. NCBI nr
Match: XP_022991905.1 (uncharacterized protein LOC111488402 [Cucurbita maxima])

HSP 1 Score: 708 bits (1828), Expect = 6.32e-257
Identity = 364/368 (98.91%), Postives = 366/368 (99.46%), Query Frame = 0

Query: 1   MARVCDLQSLSSLFASRPTIPRSSNFKPICSFHCSSSSSPFTEKHSVKRYQRDDWLYKNQ 60
           MARVCDLQSLSSLF+SRPTIPRSSNFKPICSFHCSSSSSPFTEKHSVKRYQRDDW+YKNQ
Sbjct: 1   MARVCDLQSLSSLFSSRPTIPRSSNFKPICSFHCSSSSSPFTEKHSVKRYQRDDWVYKNQ 60

Query: 61  ADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSGCDDGKCGPGNVFLV 120
           ADESSVASSCSI  DSESIRQNDIALQLPELKKLLQVLREKRASSGCDDGKCGPGNVFLV
Sbjct: 61  ADESSVASSCSISCDSESIRQNDIALQLPELKKLLQVLREKRASSGCDDGKCGPGNVFLV 120

Query: 121 GTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRTQEE 180
           GTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRTQEE
Sbjct: 121 GTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRTQEE 180

Query: 181 IHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAAELG 240
           IHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAAELG
Sbjct: 181 IHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAAELG 240

Query: 241 IPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHHG 300
           IPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHHG
Sbjct: 241 IPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHHG 300

Query: 301 LPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSK 360
           LPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSK
Sbjct: 301 LPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSK 360

Query: 361 EASSLVEA 368
           EASSLVEA
Sbjct: 361 EASSLVEA 368

BLAST of Cp4.1LG12g06870 vs. NCBI nr
Match: XP_022954326.1 (uncharacterized protein LOC111456604 [Cucurbita moschata])

HSP 1 Score: 704 bits (1817), Expect = 3.90e-255
Identity = 367/375 (97.87%), Postives = 367/375 (97.87%), Query Frame = 0

Query: 1   MARVCDLQSLSSLFASRPTIPRSSNFKPICSFHCSSSSS-------PFTEKHSVKRYQRD 60
           MARVCDLQSLSSLFASRPTIPRSSNFKPICSFHCSSSSS       PFTEKHSVKRYQRD
Sbjct: 1   MARVCDLQSLSSLFASRPTIPRSSNFKPICSFHCSSSSSSSSSSSSPFTEKHSVKRYQRD 60

Query: 61  DWLYKNQADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSGCDDGKCG 120
           DWLYKNQADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSG DDGKCG
Sbjct: 61  DWLYKNQADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSGGDDGKCG 120

Query: 121 PGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGF 180
           PGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGF
Sbjct: 121 PGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGF 180

Query: 181 HSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAAS 240
           HSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAAS
Sbjct: 181 HSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAAS 240

Query: 241 GIAAELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLA 300
           GIAAELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLA
Sbjct: 241 GIAAELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLA 300

Query: 301 LKLMHHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSP 360
           LKLMHHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSP
Sbjct: 301 LKLMHHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSP 360

Query: 361 HWSLSSKEASSLVEA 368
           HWSLSSKEASSLVEA
Sbjct: 361 HWSLSSKEASSLVEA 375

BLAST of Cp4.1LG12g06870 vs. NCBI nr
Match: KAG6575668.1 (hypothetical protein SDJN03_26307, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 702 bits (1813), Expect = 1.37e-254
Identity = 365/371 (98.38%), Postives = 366/371 (98.65%), Query Frame = 0

Query: 1   MARVCDLQSLSSLFASRPTIPRSSNFKPICSFHCSSSSS---PFTEKHSVKRYQRDDWLY 60
           MARVCDLQSLSSLF+SRPTIPRSSNFKPICSFHCSSSSS   PFTEKHSVKRYQRDDWLY
Sbjct: 1   MARVCDLQSLSSLFSSRPTIPRSSNFKPICSFHCSSSSSSSSPFTEKHSVKRYQRDDWLY 60

Query: 61  KNQADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSGCDDGKCGPGNV 120
           KNQADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKR SSG DDGKCGPGNV
Sbjct: 61  KNQADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRPSSGGDDGKCGPGNV 120

Query: 121 FLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRT 180
           FLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRT
Sbjct: 121 FLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRT 180

Query: 181 QEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAA 240
           QEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAA
Sbjct: 181 QEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAA 240

Query: 241 ELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLM 300
           ELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLM
Sbjct: 241 ELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLM 300

Query: 301 HHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSL 360
           HHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSL
Sbjct: 301 HHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSL 360

Query: 361 SSKEASSLVEA 368
           SSKEASSLVEA
Sbjct: 361 SSKEASSLVEA 371

BLAST of Cp4.1LG12g06870 vs. NCBI nr
Match: KAG7014220.1 (cysG, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 681 bits (1758), Expect = 2.18e-246
Identity = 354/360 (98.33%), Postives = 355/360 (98.61%), Query Frame = 0

Query: 12  SLFASRPTIPRSSNFKPICSFHCSSSSS---PFTEKHSVKRYQRDDWLYKNQADESSVAS 71
           SLF+SRPTIPRSSNFKPICSFHCSSSSS   PFTEKHSVKRYQRDDWLYKNQADESSVAS
Sbjct: 1   SLFSSRPTIPRSSNFKPICSFHCSSSSSSSSPFTEKHSVKRYQRDDWLYKNQADESSVAS 60

Query: 72  SCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSGCDDGKCGPGNVFLVGTGPGDPE 131
           SCSIPSDSESIRQNDIALQLPELKKLLQVLREKR SSG DDGKCGPGNVFLVGTGPGDPE
Sbjct: 61  SCSIPSDSESIRQNDIALQLPELKKLLQVLREKRPSSGGDDGKCGPGNVFLVGTGPGDPE 120

Query: 132 LLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRTQEEIHELLLNF 191
           LLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRTQEEIHELLLNF
Sbjct: 121 LLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRTQEEIHELLLNF 180

Query: 192 AEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAAELGIPLTHRGV 251
           AEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAAELGIPLTHRGV
Sbjct: 181 AEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAAELGIPLTHRGV 240

Query: 252 ATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHHGLPPDTPAA 311
           ATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHHGLPPDTPAA
Sbjct: 241 ATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHHGLPPDTPAA 300

Query: 312 AIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSKEASSLVEA 368
           AIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSKEASSLVEA
Sbjct: 301 AIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSKEASSLVEA 360

BLAST of Cp4.1LG12g06870 vs. ExPASy TrEMBL
Match: A0A6J1JU84 (uncharacterized protein LOC111488402 OS=Cucurbita maxima OX=3661 GN=LOC111488402 PE=3 SV=1)

HSP 1 Score: 708 bits (1828), Expect = 3.06e-257
Identity = 364/368 (98.91%), Postives = 366/368 (99.46%), Query Frame = 0

Query: 1   MARVCDLQSLSSLFASRPTIPRSSNFKPICSFHCSSSSSPFTEKHSVKRYQRDDWLYKNQ 60
           MARVCDLQSLSSLF+SRPTIPRSSNFKPICSFHCSSSSSPFTEKHSVKRYQRDDW+YKNQ
Sbjct: 1   MARVCDLQSLSSLFSSRPTIPRSSNFKPICSFHCSSSSSPFTEKHSVKRYQRDDWVYKNQ 60

Query: 61  ADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSGCDDGKCGPGNVFLV 120
           ADESSVASSCSI  DSESIRQNDIALQLPELKKLLQVLREKRASSGCDDGKCGPGNVFLV
Sbjct: 61  ADESSVASSCSISCDSESIRQNDIALQLPELKKLLQVLREKRASSGCDDGKCGPGNVFLV 120

Query: 121 GTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRTQEE 180
           GTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRTQEE
Sbjct: 121 GTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRTQEE 180

Query: 181 IHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAAELG 240
           IHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAAELG
Sbjct: 181 IHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAAELG 240

Query: 241 IPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHHG 300
           IPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHHG
Sbjct: 241 IPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHHG 300

Query: 301 LPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSK 360
           LPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSK
Sbjct: 301 LPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSK 360

Query: 361 EASSLVEA 368
           EASSLVEA
Sbjct: 361 EASSLVEA 368

BLAST of Cp4.1LG12g06870 vs. ExPASy TrEMBL
Match: A0A6J1GQM1 (uncharacterized protein LOC111456604 OS=Cucurbita moschata OX=3662 GN=LOC111456604 PE=3 SV=1)

HSP 1 Score: 704 bits (1817), Expect = 1.89e-255
Identity = 367/375 (97.87%), Postives = 367/375 (97.87%), Query Frame = 0

Query: 1   MARVCDLQSLSSLFASRPTIPRSSNFKPICSFHCSSSSS-------PFTEKHSVKRYQRD 60
           MARVCDLQSLSSLFASRPTIPRSSNFKPICSFHCSSSSS       PFTEKHSVKRYQRD
Sbjct: 1   MARVCDLQSLSSLFASRPTIPRSSNFKPICSFHCSSSSSSSSSSSSPFTEKHSVKRYQRD 60

Query: 61  DWLYKNQADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSGCDDGKCG 120
           DWLYKNQADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSG DDGKCG
Sbjct: 61  DWLYKNQADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSGGDDGKCG 120

Query: 121 PGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGF 180
           PGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGF
Sbjct: 121 PGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGF 180

Query: 181 HSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAAS 240
           HSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAAS
Sbjct: 181 HSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAAS 240

Query: 241 GIAAELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLA 300
           GIAAELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLA
Sbjct: 241 GIAAELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLA 300

Query: 301 LKLMHHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSP 360
           LKLMHHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSP
Sbjct: 301 LKLMHHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSP 360

Query: 361 HWSLSSKEASSLVEA 368
           HWSLSSKEASSLVEA
Sbjct: 361 HWSLSSKEASSLVEA 375

BLAST of Cp4.1LG12g06870 vs. ExPASy TrEMBL
Match: A0A1S3CDT8 (uroporphyrinogen-III C-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103499894 PE=3 SV=1)

HSP 1 Score: 669 bits (1726), Expect = 1.24e-241
Identity = 342/372 (91.94%), Postives = 355/372 (95.43%), Query Frame = 0

Query: 1   MARVCDLQSLSSLFASRPTIPRSSNFKPICSFHCS----SSSSPFTEKHSVKRYQRDDWL 60
           MAR CDLQSLSS F+S PTIPRS NFKPI SFHCS    SSSSPFTEKHS+KRYQRDDWL
Sbjct: 1   MARFCDLQSLSSPFSSHPTIPRSPNFKPIFSFHCSYSSSSSSSPFTEKHSIKRYQRDDWL 60

Query: 61  YKNQADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSGCDDGKCGPGN 120
           YKNQ+D+ SV SSCSIP DSES+RQNDIA+QLPELKKLL+VLREKR SSGCDDGKCGPGN
Sbjct: 61  YKNQSDQPSVTSSCSIPYDSESMRQNDIAMQLPELKKLLEVLREKRVSSGCDDGKCGPGN 120

Query: 121 VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSR 180
           VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVL+LVG DARLLYVGKTAG+HSR
Sbjct: 121 VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLELVGPDARLLYVGKTAGYHSR 180

Query: 181 TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIA 240
           TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIA
Sbjct: 181 TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIA 240

Query: 241 AELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKL 300
           AELGIPLTHRGVATSVRFLTGHSR+GGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKL
Sbjct: 241 AELGIPLTHRGVATSVRFLTGHSRKGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKL 300

Query: 301 MHHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWS 360
           MHHGLPPDTPAAA+ERGTTPQQR VFA LKDLADEIKAAELVSPTLI+IG+VVSLSPHWS
Sbjct: 301 MHHGLPPDTPAAAVERGTTPQQRTVFAHLKDLADEIKAAELVSPTLIVIGRVVSLSPHWS 360

Query: 361 LSSKEASSLVEA 368
           LSS EASSLVEA
Sbjct: 361 LSSNEASSLVEA 372

BLAST of Cp4.1LG12g06870 vs. ExPASy TrEMBL
Match: A0A0A0KBI8 (TP_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G428980 PE=3 SV=1)

HSP 1 Score: 664 bits (1713), Expect = 1.19e-239
Identity = 340/372 (91.40%), Postives = 355/372 (95.43%), Query Frame = 0

Query: 1   MARVCDLQSLSSLFASRPTIPRSSNFKPICSFHCSS----SSSPFTEKHSVKRYQRDDWL 60
           MAR CDLQSLSS F+S PTIPRS NFKPI SFHCSS    SSSPFTEKHSVKRYQRDDWL
Sbjct: 1   MARFCDLQSLSSPFSSHPTIPRSPNFKPIFSFHCSSASSSSSSPFTEKHSVKRYQRDDWL 60

Query: 61  YKNQADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSGCDDGKCGPGN 120
           YK Q+D+ SV SSCSIP DSESIRQNDIA+QLPELKKLL+VLREKR S+GCDDGKCGPG+
Sbjct: 61  YKYQSDQPSVTSSCSIPYDSESIRQNDIAMQLPELKKLLEVLREKRVSNGCDDGKCGPGD 120

Query: 121 VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSR 180
           VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVL+LVG DARLLYVGKTAG+HSR
Sbjct: 121 VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLELVGPDARLLYVGKTAGYHSR 180

Query: 181 TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIA 240
           TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIA
Sbjct: 181 TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIA 240

Query: 241 AELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKL 300
           AELGIPLTHRGVATSVRFLTGHSR+GGTDPL+VAENAADPDSTLVVYMGLSTLPSLALKL
Sbjct: 241 AELGIPLTHRGVATSVRFLTGHSRKGGTDPLYVAENAADPDSTLVVYMGLSTLPSLALKL 300

Query: 301 MHHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWS 360
           MHHGLPPDTPAAA+ERGTTPQQR VFA+LKDLADEIKAAELVSPTLI+IG+VVSLSPHWS
Sbjct: 301 MHHGLPPDTPAAAVERGTTPQQRTVFAQLKDLADEIKAAELVSPTLIVIGRVVSLSPHWS 360

Query: 361 LSSKEASSLVEA 368
           LSS EASSLVEA
Sbjct: 361 LSSNEASSLVEA 372

BLAST of Cp4.1LG12g06870 vs. ExPASy TrEMBL
Match: A0A6J1D4K3 (uncharacterized protein LOC111017535 OS=Momordica charantia OX=3673 GN=LOC111017535 PE=3 SV=1)

HSP 1 Score: 649 bits (1673), Expect = 1.32e-233
Identity = 333/369 (90.24%), Postives = 350/369 (94.85%), Query Frame = 0

Query: 1   MARVCDLQSLSSLFASRPTIPRSSNFKPICSFHCSSSSSPFTEKHSVKRYQRDDWLYKNQ 60
           MARV DLQSLSS F+SRP  PR+  FKPI S HCSSSSSPFTEKHS+KRYQRDDW+YKNQ
Sbjct: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHCSSSSSPFTEKHSIKRYQRDDWVYKNQ 60

Query: 61  ADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSGCDDG-KCGPGNVFL 120
           AD++S A SCS+P DS+SIRQ+DIALQLPELK+LL VLREKR + GCDDG +CGPGNVFL
Sbjct: 61  ADQASTALSCSVPCDSDSIRQDDIALQLPELKRLLMVLREKRVNGGCDDGGECGPGNVFL 120

Query: 121 VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRTQE 180
           VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVG DARLLYVGKTAG+HSRTQE
Sbjct: 121 VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQE 180

Query: 181 EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAAEL 240
           EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQ+GIQVKIVPGITAASGIAAEL
Sbjct: 181 EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAEL 240

Query: 241 GIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHH 300
           GIPLTHRGVAT+VRFLTGHSR+GGTDPLFVAENAAD DSTLVVYMGLSTLPSLALKLMHH
Sbjct: 241 GIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADLDSTLVVYMGLSTLPSLALKLMHH 300

Query: 301 GLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS 360
           GLPPDTPAAA+ERGTTPQQR VFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS
Sbjct: 301 GLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS 360

Query: 361 KEASSLVEA 368
           KEAS LVEA
Sbjct: 361 KEASCLVEA 369

BLAST of Cp4.1LG12g06870 vs. TAIR 10
Match: AT5G40850.1 (urophorphyrin methylase 1 )

HSP 1 Score: 522.3 bits (1344), Expect = 3.1e-148
Identity = 274/347 (78.96%), Postives = 295/347 (85.01%), Query Frame = 0

Query: 24  SNFKPICSFH---CSSSSSPFTEKHSVKRYQRDDWLYKNQADESSVASSCSIPSDSESIR 83
           +N  PIC  H    SSSSSPFTEKHSV+RYQRD WLYK          S S   D   +R
Sbjct: 22  TNLTPICCLHYNTASSSSSPFTEKHSVERYQRDQWLYKAVEPTPPSTPSPSPFEDEVFVR 81

Query: 84  QNDIALQLPELKKLLQVLREKRASSGCDDGKCGPGNVFLVGTGPGDPELLTLKAVKVIQS 143
           +NDIA QLPELKKLL VL+EKR   GC  G CGPG+V+LVGTGPGDPELLTLKAV+VIQS
Sbjct: 82  ENDIASQLPELKKLLAVLKEKRV-KGCKGGDCGPGDVYLVGTGPGDPELLTLKAVRVIQS 141

Query: 144 ADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRTQEEIHELLLNFAEAGATVVRLKG 203
           ADLLLYDRLVSNDVL+LV  DARLLYVGKTAG+HSRTQEEIHELLLNFAEAGATVVRLKG
Sbjct: 142 ADLLLYDRLVSNDVLELVAPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKG 201

Query: 204 GDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAAELGIPLTHRGVATSVRFLTGHSR 263
           GDPLVFGRGGEEMDFLQQQGI+V+++PGITAASGIAAELGIPLTHRGVATSVRFLTGHSR
Sbjct: 202 GDPLVFGRGGEEMDFLQQQGIRVQVIPGITAASGIAAELGIPLTHRGVATSVRFLTGHSR 261

Query: 264 QGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHHGLPPDTPAAAIERGTTPQQRI 323
           +GGTDPLFVAENAADPD+TLVVYMGL TLPSLA KLM HGLP DTPA A+ERGTTP QR 
Sbjct: 262 KGGTDPLFVAENAADPDTTLVVYMGLGTLPSLAQKLMDHGLPSDTPAVAVERGTTPLQRT 321

Query: 324 VFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSKEASSLVE 368
           VFAELKD A EI++A LVSPTLIIIGKVV LSP W   +KE+S LVE
Sbjct: 322 VFAELKDFATEIQSAGLVSPTLIIIGKVVELSPLWPHCTKESSCLVE 367

BLAST of Cp4.1LG12g06870 vs. TAIR 10
Match: AT5G40850.2 (urophorphyrin methylase 1 )

HSP 1 Score: 467.2 bits (1201), Expect = 1.2e-131
Identity = 244/317 (76.97%), Postives = 263/317 (82.97%), Query Frame = 0

Query: 24  SNFKPICSFH---CSSSSSPFTEKHSVKRYQRDDWLYKNQADESSVASSCSIPSDSESIR 83
           +N  PIC  H    SSSSSPFTEKHSV+RYQRD WLYK          S S   D   +R
Sbjct: 22  TNLTPICCLHYNTASSSSSPFTEKHSVERYQRDQWLYKAVEPTPPSTPSPSPFEDEVFVR 81

Query: 84  QNDIALQLPELKKLLQVLREKRASSGCDDGKCGPGNVFLVGTGPGDPELLTLKAVKVIQS 143
           +NDIA QLPELKKLL VL+EKR   GC  G CGPG+V+LVGTGPGDPELLTLKAV+VIQS
Sbjct: 82  ENDIASQLPELKKLLAVLKEKRV-KGCKGGDCGPGDVYLVGTGPGDPELLTLKAVRVIQS 141

Query: 144 ADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRTQEEIHELLLNFAEAGATVVRLKG 203
           ADLLLYDRLVSNDVL+LV  DARLLYVGKTAG+HSRTQEEIHELLLNFAEAGATVVRLKG
Sbjct: 142 ADLLLYDRLVSNDVLELVAPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKG 201

Query: 204 GDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAAELGIPLTHRGVATSVRFLTGHSR 263
           GDPLVFGRGGEEMDFLQQQGI+V+++PGITAASGIAAELGIPLTHRGVATSVRFLTGHSR
Sbjct: 202 GDPLVFGRGGEEMDFLQQQGIRVQVIPGITAASGIAAELGIPLTHRGVATSVRFLTGHSR 261

Query: 264 QGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHHGLPPDTPAAAIERGTTPQQRI 323
           +GGTDPLFVAENAADPD+TLVVYMGL TLPSLA KLM HGLP DTPA A+ERGTTP QR 
Sbjct: 262 KGGTDPLFVAENAADPDTTLVVYMGLGTLPSLAQKLMDHGLPSDTPAVAVERGTTPLQRT 321

Query: 324 VFAELKDLADEIKAAEL 338
            F  L      +K  +L
Sbjct: 322 AFGILCRFLLSLKILQL 337

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q426064.4e-14778.96S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase, chloro... [more]
B8GUD35.1e-6349.60Siroheme synthase OS=Thioalkalivibrio sulfidiphilus (strain HL-EbGR7) OX=396588 ... [more]
A0KP374.3e-6252.38Siroheme synthase 2 OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 ... [more]
Q0VQ051.3e-6150.21Siroheme synthase OS=Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / N... [more]
Q6F8G62.2e-6152.32Siroheme synthase OS=Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) OX=... [more]
Match NameE-valueIdentityDescription
XP_023548332.18.02e-260100.00uncharacterized protein LOC111807000 [Cucurbita pepo subsp. pepo][more]
XP_022991905.16.32e-25798.91uncharacterized protein LOC111488402 [Cucurbita maxima][more]
XP_022954326.13.90e-25597.87uncharacterized protein LOC111456604 [Cucurbita moschata][more]
KAG6575668.11.37e-25498.38hypothetical protein SDJN03_26307, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7014220.12.18e-24698.33cysG, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
A0A6J1JU843.06e-25798.91uncharacterized protein LOC111488402 OS=Cucurbita maxima OX=3661 GN=LOC111488402... [more]
A0A6J1GQM11.89e-25597.87uncharacterized protein LOC111456604 OS=Cucurbita moschata OX=3662 GN=LOC1114566... [more]
A0A1S3CDT81.24e-24191.94uroporphyrinogen-III C-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103499894... [more]
A0A0A0KBI81.19e-23991.40TP_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G42898... [more]
A0A6J1D4K31.32e-23390.24uncharacterized protein LOC111017535 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
Match NameE-valueIdentityDescription
AT5G40850.13.1e-14878.96urophorphyrin methylase 1 [more]
AT5G40850.21.2e-13176.97urophorphyrin methylase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014777Tetrapyrrole methylase, subdomain 1GENE3D3.40.1010.10coord: 108..230
e-value: 2.2E-41
score: 142.9
IPR006366Uroporphyrin-III C-methyltransferaseTIGRFAMTIGR01469TIGR01469coord: 115..351
e-value: 3.8E-87
score: 289.9
IPR006366Uroporphyrin-III C-methyltransferaseCDDcd11642SUMTcoord: 120..349
e-value: 6.13007E-125
score: 357.132
IPR014776Tetrapyrrole methylase, subdomain 2GENE3D3.30.950.10coord: 231..367
e-value: 5.1E-45
score: 154.8
IPR000878Tetrapyrrole methylasePFAMPF00590TP_methylasecoord: 117..329
e-value: 8.6E-51
score: 172.9
NoneNo IPR availablePANTHERPTHR45790SIROHEME SYNTHASE-RELATEDcoord: 111..366
NoneNo IPR availablePANTHERPTHR45790:SF3UROPORPHYRINOGEN-III C-METHYLTRANSFERASEcoord: 111..366
IPR003043Uroporphiryn-III C-methyltransferase, conserved sitePROSITEPS00840SUMT_2coord: 195..228
IPR003043Uroporphiryn-III C-methyltransferase, conserved sitePROSITEPS00839SUMT_1coord: 120..134
IPR035996Tetrapyrrole methylase superfamilySUPERFAMILY53790Tetrapyrrole methylasecoord: 114..355

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG12g06870.1Cp4.1LG12g06870.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0019354 siroheme biosynthetic process
molecular_function GO:0004851 uroporphyrin-III C-methyltransferase activity
molecular_function GO:0008168 methyltransferase activity