Cp4.1LG12g06330 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG12g06330 vs. ExPASy Swiss-Prot
Match: Q93XK2 (Stachyose synthase OS=Pisum sativum OX=3888 GN=STS1 PE=1 SV=1)

HSP 1 Score: 1169.1 bits (3023), Expect = 0.0e+00
Identity = 543/843 (64.41%), Postives = 667/843 (79.12%), Query Frame = 0

Query: 10  LSNGNISVQGVPLLSDVPSNVFFSPFSSV---RSSDAPLPLFQRVHALSHKGGFLGFDQT 69
           LS     V+G PL  DVP NV F  FSS+     S+AP  L Q+V A SHKGGF GF  +
Sbjct: 21  LSERKFKVKGFPLFHDVPENVSFRSFSSICKPSESNAPPSLLQKVLAYSHKGGFFGF--S 80

Query: 70  QSQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPEIRSYV 129
              PSDRL+NS+G F G++F+SIFRFKTWWST W+G SGSD+QMETQW+++ VPE +SYV
Sbjct: 81  HETPSDRLMNSIGSFNGKDFLSIFRFKTWWSTQWIGKSGSDLQMETQWILIEVPETKSYV 140

Query: 130 VIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVCDNPYNLMKEAYAT 189
           VIIPIIE  FRSAL PG + HV I AESGST+VK S+F++IAY+H  +NPY+LMKEAY+ 
Sbjct: 141 VIIPIIEKCFRSALFPGFNDHVKIIAESGSTKVKESTFNSIAYVHFSENPYDLMKEAYSA 200

Query: 190 IRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGVNDFFEGGVSLRFLIID 249
           IRVHLN+FRLLEEK++P+LVD+FGWCTW+AF+L VNP+G+++G++DF +GGV  RF+IID
Sbjct: 201 IRVHLNSFRLLEEKTIPNLVDKFGWCTWDAFYLTVNPIGIFHGLDDFSKGGVEPRFVIID 260

Query: 250 DGWQSINIDGEDPHRDMKYIVLGANQMTARLYKFEECDKFEKYKGGTMLGPNAPPFDPKK 309
           DGWQSI+ DG DP+ D K +VLG  QM+ RL++F+EC KF KY+ G +LGPN+PP+DP  
Sbjct: 261 DGWQSISFDGYDPNEDAKNLVLGGEQMSGRLHRFDECYKFRKYESGLLLGPNSPPYDPNN 320

Query: 310 PKLLISKSIEIDNAEKNRDNAIQSGVNDVFKFETEVKKLKQELIDIFWNDRDEENNVESN 369
              LI K IE +   K R+ AI S  +D+ + E+++KK+ +E+ D+F          E  
Sbjct: 321 FTDLILKGIEHEKLRKKREEAISSKSSDLAEIESKIKKVVKEIDDLFGG--------EQF 380

Query: 370 GNGNCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALTGAWGGVRPGSTHLKSKITPLKS 429
            +G       + G+KAFT+DLRTKFKGLDD+YVWHAL GAWGGVRP +THL +KI P K 
Sbjct: 381 SSGEKSEMKSEYGLKAFTKDLRTKFKGLDDVYVWHALCGAWGGVRPETTHLDTKIVPCKL 440

Query: 430 SPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLFDSMHSYLSEAGITGVKVDVIHTLEYVS 489
           SPGLDGTM DLAVV++ +  +GLVHP QA +L+DSMHSYL+E+GITGVKVDVIH+LEYV 
Sbjct: 441 SPGLDGTMEDLAVVEISKASLGLVHPSQANELYDSMHSYLAESGITGVKVDVIHSLEYVC 500

Query: 490 EEYGGRVDLAKTYYKGLTDSIVKNFKGTNLLSSMQQCNDFFFLGTKQNSIGRASDDFWFH 549
           +EYGGRVDLAK YY+GLT SIVKNF G  +++SMQ CNDFFFLGTKQ S+GR  DDFWF 
Sbjct: 501 DEYGGRVDLAKVYYEGLTKSIVKNFNGNGMIASMQHCNDFFFLGTKQISMGRVGDDFWFQ 560

Query: 550 DPHGDPMGMYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGSRAICGGPIY 609
           DP+GDPMG +WLQGVHM+HC+YNS+WMGQ+IQPDWDMFQSDHVCAKFHAGSRAICGGPIY
Sbjct: 561 DPNGDPMGSFWLQGVHMIHCSYNSLWMGQMIQPDWDMFQSDHVCAKFHAGSRAICGGPIY 620

Query: 610 VSDSVGSHDFYLIKKLAYPDGTIPRCRHFALPTRDCLFKNPLFDNKTVLKIWNLNMYGGV 669
           VSD+VGSHDF LIKKL +PDGTIP+C +F LPTRDCLFKNPLFD+ TVLKIWN N YGGV
Sbjct: 621 VSDNVGSHDFDLIKKLVFPDGTIPKCIYFPLPTRDCLFKNPLFDHTTVLKIWNFNKYGGV 680

Query: 670 IGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPEAAPMGNFVEYVVYLNQ 729
           IGAFNCQGAGWD   ++ +G PECYKP+  TVHV +VEWDQK E + +G   EYVVYLNQ
Sbjct: 681 IGAFNCQGAGWDPIMQKFRGFPECYKPIPGTVHVTEVEWDQKEETSHLGKAEEYVVYLNQ 740

Query: 730 AEQILHTTPKSEPLELNLEPSTFELFNFIPLKKLDSNIKFAPIGLTNMFNSSGTIQHLKY 789
           AE++   T KSEP++  ++PSTFEL++F+P+ KL   IKFAPIGLTNMFNS GT+  L+Y
Sbjct: 741 AEELSLMTLKSEPIQFTIQPSTFELYSFVPVTKLCGGIKFAPIGLTNMFNSGGTVIDLEY 800

Query: 790 NKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDYDGKLSFDLCWTEEAGGFSNLD 849
             NG ++KVKGGG+FLAYSS SPKK   NG EV+FEW  DGKL  ++ W EEA G S+++
Sbjct: 801 VGNGAKIKVKGGGSFLAYSSESPKKFQLNGCEVDFEWLGDGKLCVNVPWIEEACGVSDME 853

BLAST of Cp4.1LG12g06330 vs. ExPASy Swiss-Prot
Match: Q9SYJ4 (Probable galactinol--sucrose galactosyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=RFS4 PE=2 SV=3)

HSP 1 Score: 1041.2 bits (2691), Expect = 6.5e-303
Identity = 500/857 (58.34%), Postives = 642/857 (74.91%), Query Frame = 0

Query: 6   NYVHLSNGNISVQ-GVPLLSDVPSNVFFSPFSSVR-SSDAPLPLFQRVHALSHKGGFLGF 65
           N  +LS G++  +   P+L DVP NV F+PFSS   S+DAPLP+  RV A +HKGGFLGF
Sbjct: 32  NSFNLSEGSLCAKDSTPILFDVPQNVTFTPFSSHSISTDAPLPILLRVQANAHKGGFLGF 91

Query: 66  DQTQSQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPEIR 125
             T+  PSDRL NSLG+FE REF+S+FRFK WWST W+G SGSD+Q ETQWV+L +PEI 
Sbjct: 92  --TKESPSDRLTNSLGRFEDREFLSLFRFKMWWSTAWIGKSGSDLQAETQWVMLKIPEID 151

Query: 126 SYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVCDNPYNLMKEA 185
           SYV IIP IEG+FR++L PG  G+V+I AESGST+VK SSF +IAYIH+CDNPYNLMKEA
Sbjct: 152 SYVAIIPTIEGAFRASLTPGEKGNVLICAESGSTKVKESSFKSIAYIHICDNPYNLMKEA 211

Query: 186 YATIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGVNDFFEGGVSLRFL 245
           ++ +RVH+NTF+LLEEK +P +VD+FGWCTW+A +L V+P  +W GV +F +GGV  +F+
Sbjct: 212 FSALRVHMNTFKLLEEKKLPKIVDKFGWCTWDACYLTVDPATIWTGVKEFEDGGVCPKFV 271

Query: 246 IIDDGWQSINIDGEDPHRDMKYIVLGANQMTARLYKFEECDKFEKYKGGTMLGPNAPPFD 305
           IIDDGWQSIN DG++  +D + +VLG  QMTARL  F+EC KF  YKGG+ +  +A  F+
Sbjct: 272 IIDDGWQSINFDGDELDKDAENLVLGGEQMTARLTSFKECKKFRNYKGGSFITSDASHFN 331

Query: 306 PKKPKLLISKSIE-IDNAEKNRDNAIQSGVNDVFKFETEVKKLKQELIDIFWNDRDEENN 365
           P KPK+LI K+ E I      R    +SG  D+ + + ++K L +EL  +F    DE   
Sbjct: 332 PLKPKMLIYKATERIQAIILRRKLVKESGEQDLTELDEKIKILSEELNAMF----DEVEK 391

Query: 366 VESNGNGNCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALTGAWGGVRPGS-THLKSKI 425
            ES G+ +        GM AFT+DLR +FK LDDIYVWHAL GAW GVRP +   LK+K+
Sbjct: 392 EESLGSDDV----SGSGMAAFTKDLRLRFKSLDDIYVWHALCGAWNGVRPETMMDLKAKV 451

Query: 426 TPLKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLFDSMHSYLSEAGITGVKVDVIHT 485
            P + SP L  TM DLAV KV+E  IGLVHP +A++ +DSMHSYL+  G+TG K+DV  T
Sbjct: 452 APFELSPSLGATMADLAVDKVVEAGIGLVHPSKAHEFYDSMHSYLASVGVTGAKIDVFQT 511

Query: 486 LEYVSEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLLSSMQQCNDFFFLGTKQNSIGRASD 545
           LE ++EE+GGRV+LAK YY GLT+S++KNF GT++++SMQQCN+FFFL TKQ SIGR  D
Sbjct: 512 LESLAEEHGGRVELAKAYYDGLTESMIKNFNGTDVIASMQQCNEFFFLATKQISIGRVGD 571

Query: 546 DFWFHDPHGDPMGMYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGSRAIC 605
           DFW+ DP+GDP G+YWLQGVHM+HC+YNS+WMGQ+IQPDWDMFQSDHVCA++HA SRAIC
Sbjct: 572 DFWWQDPYGDPQGVYWLQGVHMIHCSYNSIWMGQMIQPDWDMFQSDHVCAEYHAASRAIC 631

Query: 606 GGPIYVSDSVG--SHDFYLIKKLAYPDGTIPRCRHFALPTRDCLFKNPLFDNKTVLKIWN 665
           GGP+Y+SD +G  SH+F LIKKLA+ DGTIPRC H+ALPTRD LFKNPLFD +++LKI+N
Sbjct: 632 GGPVYLSDHLGKASHNFDLIKKLAFFDGTIPRCVHYALPTRDSLFKNPLFDKESILKIFN 691

Query: 666 LNMYGGVIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPEAAPMGNFV- 725
            N +GGVIG FNCQGAGW  +E R KG+ ECY  +S TVHV+D+EWDQ PEAA  G+ V 
Sbjct: 692 FNKFGGVIGTFNCQGAGWSPEEHRFKGYKECYTTVSGTVHVSDIEWDQNPEAA--GSQVT 751

Query: 726 ---EYVVYLNQAEQILHTTPKSEPLELNLEPSTFELFNFIPLKKL-DSNIKFAPIGLTNM 785
              +Y+VY  Q+E+IL    KSE +++ LEPS F+L +F+P+ +L  S ++FAP+GL NM
Sbjct: 752 YTGDYLVYKQQSEEILFMNSKSEAMKITLEPSAFDLLSFVPVTELVSSGVRFAPLGLINM 811

Query: 786 FNSSGTIQHLKY-NKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDYD-GKLSFD 845
           FN  GT+Q +K    N + + VKG G F+AYSS +P KC  N  E EF+W+ + GKLSF 
Sbjct: 812 FNCVGTVQDMKVTGDNSIRVDVKGEGRFMAYSSSAPVKCYLNDKEAEFKWEEETGKLSFF 871

Query: 846 LCWTEEAGGFSNLDIFF 850
           + W EE+GG S+L   F
Sbjct: 872 VPWVEESGGISHLSFTF 876

BLAST of Cp4.1LG12g06330 vs. ExPASy Swiss-Prot
Match: Q9FND9 (Probable galactinol--sucrose galactosyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=RFS5 PE=1 SV=1)

HSP 1 Score: 718.4 bits (1853), Expect = 9.6e-206
Identity = 380/853 (44.55%), Postives = 497/853 (58.26%), Query Frame = 0

Query: 10  LSNGNISVQGVPLLSDVPSNVFF--SPFSSVRSSDAPLPLFQRVHALSHKGGFLGFDQTQ 69
           L +  +   G  +L+DVP NV    SP+  V     PL +          G F+GF+   
Sbjct: 24  LEDSTLLANGQVVLTDVPVNVTLTSSPY-LVDKDGVPLDV--------SAGSFIGFN-LD 83

Query: 70  SQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVP------- 129
            +P    + S+GK +   F+SIFRFK WW+T WVGS+G DI+ ETQ +IL+         
Sbjct: 84  GEPKSHHVASIGKLKNIRFMSIFRFKVWWTTHWVGSNGRDIENETQIIILDQSGSDSGPG 143

Query: 130 --EIRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVCDNPYN 189
               R YV+++P++EGSFRS+   G D  V +  ESGST+V  S F  I Y+H  D+P+ 
Sbjct: 144 SGSGRPYVLLLPLLEGSFRSSFQSGEDDDVAVCVESGSTEVTGSEFRQIVYVHAGDDPFK 203

Query: 190 LMKEAYATIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGVNDFFEGGV 249
           L+K+A   IRVH+NTF+LLEEKS P +VD+FGWCTW+AF+L VNP GV  GV    +GG 
Sbjct: 204 LVKDAMKVIRVHMNTFKLLEEKSPPGIVDKFGWCTWDAFYLTVNPDGVHKGVKCLVDGGC 263

Query: 250 SLRFLIIDDGWQSINIDGEDPHRDMKYIVLGANQMTARLYKFEECDKFEKYKGGTMLGPN 309
               ++IDDGWQSI  D +    +   I +   QM  RL KFEE  KF+ Y         
Sbjct: 264 PPGLVLIDDGWQSIGHDSDGIDVEGMNITVAGEQMPCRLLKFEENHKFKDY--------- 323

Query: 310 APPFDPKKPKLLISKSIEIDNAEKNRDNAIQSGVNDVFKFETEVKKLKQELIDIFWNDRD 369
                PK                                                     
Sbjct: 324 ---VSPKDQ--------------------------------------------------- 383

Query: 370 EENNVESNGNGNCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALTGAWGGVRPGSTHL- 429
                            +D+GMKAF RDL+ +F  +D IYVWHAL G WGG+RP +  L 
Sbjct: 384 -----------------NDVGMKAFVRDLKDEFSTVDYIYVWHALCGYWGGLRPEAPALP 443

Query: 430 KSKITPLKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLFDSMHSYLSEAGITGVKVD 489
            S I   + SPGL  TM DLAV K+IE  IG   PD A + ++ +HS+L  AGI GVKVD
Sbjct: 444 PSTIIRPELSPGLKLTMEDLAVDKIIETGIGFASPDLAKEFYEGLHSHLQNAGIDGVKVD 503

Query: 490 VIHTLEYVSEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLLSSMQQCNDFFFLGTKQNSIG 549
           VIH LE + ++YGGRVDLAK Y+K LT S+ K+F G  +++SM+ CNDF FLGT+  S+G
Sbjct: 504 VIHILEMLCQKYGGRVDLAKAYFKALTSSVNKHFNGNGVIASMEHCNDFMFLGTEAISLG 563

Query: 550 RASDDFWFHDPHGDPMGMYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGS 609
           R  DDFW  DP GDP G +WLQG HMVHCAYNS+WMG  IQPDWDMFQS H CA+FHA S
Sbjct: 564 RVGDDFWCTDPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIQPDWDMFQSTHPCAEFHAAS 623

Query: 610 RAICGGPIYVSDSVGSHDFYLIKKLAYPDGTIPRCRHFALPTRDCLFKNPLFDNKTVLKI 669
           RAI GGPIY+SD VG HDF L+K+L  P+G+I RC ++ALPTRD LF++PL D KT+LKI
Sbjct: 624 RAISGGPIYISDCVGKHDFDLLKRLVLPNGSILRCEYYALPTRDRLFEDPLHDGKTMLKI 683

Query: 670 WNLNMYGGVIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPEAAPMGNF 729
           WNLN Y GVIGAFNCQG GW R+ RR +   EC   ++ T    DVEW+       + N 
Sbjct: 684 WNLNKYTGVIGAFNCQGGGWCRETRRNQCFSECVNTLTATTSPKDVEWNSGSSPISIANV 743

Query: 730 VEYVVYLNQAEQILHTTPKSEPLELNLEPSTFELFNFIPLKKLDSN-IKFAPIGLTNMFN 789
            E+ ++L+Q++++L  +  ++ LEL LEP  FEL    P+  ++ N ++FAPIGL NM N
Sbjct: 744 EEFALFLSQSKKLL-LSGLNDDLELTLEPFKFELITVSPVVTIEGNSVRFAPIGLVNMLN 783

Query: 790 SSGTIQHLKYNKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDYDGKLSFDLCWT 849
           +SG I+ L YN   VE+ V G G F  Y+S  P  CL +G  VEF ++ D  +   + W+
Sbjct: 804 TSGAIRSLVYNDESVEVGVFGAGEFRVYASKKPVSCLIDGEVVEFGYE-DSMVMVQVPWS 783

BLAST of Cp4.1LG12g06330 vs. ExPASy Swiss-Prot
Match: Q8VWN6 (Galactinol--sucrose galactosyltransferase OS=Pisum sativum OX=3888 GN=RFS PE=1 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 1.2e-200
Identity = 366/830 (44.10%), Postives = 491/830 (59.16%), Query Frame = 0

Query: 14  NISVQGVPLLSDVPSNVFF------SPFSSVRSSDAPLPLFQRVHALSHKGGFLGFDQTQ 73
           N  V G P L+ VP N+        SPF   +S+   +      + L  +G F+GF+ T+
Sbjct: 36  NFLVNGHPFLTQVPPNITTTTTSTPSPFLDFKSNKDTIA--NNNNTLQQQGCFVGFNTTE 95

Query: 74  SQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVIL--NVPEIRSY 133
           ++     +  LGK +G +F SIFRFK WW+T WVG++G ++Q ETQ +IL  N+   R Y
Sbjct: 96  AK--SHHVVPLGKLKGIKFTSIFRFKVWWTTHWVGTNGHELQHETQILILDKNISLGRPY 155

Query: 134 VVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVCDNPYNLMKEAYA 193
           V+++PI+E SFR++L PG + +V +  ESGST V  S+F A  Y+H+ ++PY L+KEA  
Sbjct: 156 VLLLPILENSFRTSLQPGLNDYVDMSVESGSTHVTGSTFKACLYLHLSNDPYRLVKEAVK 215

Query: 194 TIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGVNDFFEGGVSLRFLII 253
            I+  L TF+ LEEK+ P ++++FGWCTW+AF+L V+P GVW GV    +GG    F+II
Sbjct: 216 VIQTKLGTFKTLEEKTPPSIIEKFGWCTWDAFYLKVHPKGVWEGVKALTDGGCPPGFVII 275

Query: 254 DDGWQSINIDGEDP--HRDMKYIVLGANQMTARLYKFEECDKFEKYKGGTMLGPNAPPFD 313
           DDGWQSI+ D +DP   RD         QM  RL K+EE  KF +Y+ G           
Sbjct: 276 DDGWQSISHDDDDPVTERDGMNRTSAGEQMPCRLIKYEENYKFREYENG----------- 335

Query: 314 PKKPKLLISKSIEIDNAEKNRDNAIQSGVNDVFKFETEVKKLKQELIDIFWNDRDEENNV 373
                                DN                                     
Sbjct: 336 ---------------------DN------------------------------------- 395

Query: 374 ESNGNGNCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALTGAWGGVRPGSTHL-KSKIT 433
                      G   G+  F RDL+ +F+ ++ +YVWHAL G WGGVRP    + ++K+ 
Sbjct: 396 -----------GGKKGLVGFVRDLKEEFRSVESVYVWHALCGYWGGVRPKVCGMPEAKVV 455

Query: 434 PLKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLFDSMHSYLSEAGITGVKVDVIHTL 493
             K SPG+  TM DLAV K++E  +GLV P+ A ++FD +HS+L  AGI GVKVDVIH L
Sbjct: 456 VPKLSPGVKMTMEDLAVDKIVENGVGLVPPNLAQEMFDGIHSHLESAGIDGVKVDVIHLL 515

Query: 494 EYVSEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLLSSMQQCNDFFFLGTKQNSIGRASDD 553
           E +SEEYGGRV+LAK YYK LT S+ K+FKG  +++SM+ CNDFF LGT+  S+GR  DD
Sbjct: 516 ELLSEEYGGRVELAKAYYKALTSSVNKHFKGNGVIASMEHCNDFFLLGTEAISLGRVGDD 575

Query: 554 FWFHDPHGDPMGMYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGSRAICG 613
           FW  DP GDP G YWLQG HMVHCAYNS+WMG  I PDWDMFQS H CA+FHA SRAI G
Sbjct: 576 FWCCDPSGDPNGTYWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAEFHAASRAISG 635

Query: 614 GPIYVSDSVGSHDFYLIKKLAYPDGTIPRCRHFALPTRDCLFKNPLFDNKTVLKIWNLNM 673
           GP+YVSD VG+H+F L+K    PDG+I RC+H+ALPTRDCLF++PL + KT+LKIWNLN 
Sbjct: 636 GPVYVSDCVGNHNFKLLKSFVLPDGSILRCQHYALPTRDCLFEDPLHNGKTMLKIWNLNK 695

Query: 674 YGGVIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPEAAPMGNFVEYVV 733
           Y GV+G FNCQG GW  + RR K   E    ++      D+EW        +     + V
Sbjct: 696 YAGVLGLFNCQGGGWCPETRRNKSASEFSHAVTCYASPEDIEWCNGKTPMDIKGVDVFAV 755

Query: 734 YLNQAEQILHTTPKSEPLELNLEPSTFELFNFIPLKKLDSN-IKFAPIGLTNMFNSSGTI 793
           Y  + E+ L     S+ LE++LEP +FEL    PLK      I+FAPIGL NM NS G +
Sbjct: 756 YFFK-EKKLSLMKCSDRLEVSLEPFSFELMTVSPLKVFSKRLIQFAPIGLVNMLNSGGAV 778

Query: 794 QHLKYNKNG--VELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDYDGKL 830
           Q L+++ +   V++ V+G G    ++S  P  C  +G+ VEF  DY+ K+
Sbjct: 816 QSLEFDDSASLVKIGVRGCGELSVFASEKPVCCKIDGVSVEF--DYEDKM 778

BLAST of Cp4.1LG12g06330 vs. ExPASy Swiss-Prot
Match: Q5VQG4 (Galactinol--sucrose galactosyltransferase OS=Oryza sativa subsp. japonica OX=39947 GN=RFS PE=1 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 8.2e-197
Identity = 362/859 (42.14%), Postives = 495/859 (57.63%), Query Frame = 0

Query: 10  LSNGNISVQGVPLLSDVPSNVFFSPFSS-VRSSDAPLPLFQRVHALSHKGGFLGFDQTQS 69
           L   +++V G P L DVP+N+  +P S+ V +SD P          +  G FLGFD   +
Sbjct: 30  LKGKDLAVDGHPFLLDVPANIRLTPASTLVPNSDVP---------AAAAGSFLGFDAPAA 89

Query: 70  QPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPEIRS---- 129
           +  DR +  +GK     F+SIFRFK WW+T WVG++G D++ ETQ +IL+    +S    
Sbjct: 90  K--DRHVVPIGKLRDTRFMSIFRFKVWWTTHWVGTNGRDVENETQMMILDQSGTKSSPTG 149

Query: 130 ---YVVIIPIIEGSFRSALHPG-TDGHVMIWAESGSTQVKASSFDAIAYIHVCDNPYNLM 189
              YV+++PI+EG FR+ L  G  + +V +  ESGS+ V+ S F +  Y+H  D+P++L+
Sbjct: 150 PRPYVLLLPIVEGPFRACLESGKAEDYVHMVLESGSSTVRGSVFRSAVYLHAGDDPFDLV 209

Query: 190 KEAYATIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGVNDFFEGGVSL 249
           K+A   +R HL TFRL+EEK+ P +VD+FGWCTW+AF+L V+P GVW GV    +GG   
Sbjct: 210 KDAMRVVRAHLGTFRLMEEKTPPPIVDKFGWCTWDAFYLKVHPEGVWEGVRRLADGGCPP 269

Query: 250 RFLIIDDGWQSINIDGEDPHRDMKYI--VLGANQMTARLYKFEECDKFEKYKGGTMLGPN 309
             ++IDDGWQSI  D +D     + +       QM  RL KF+E  KF +YKG       
Sbjct: 270 GLVLIDDGWQSICHDDDDLGSGAEGMNRTSAGEQMPCRLIKFQENYKFREYKG------- 329

Query: 310 APPFDPKKPKLLISKSIEIDNAEKNRDNAIQSGVNDVFKFETEVKKLKQELIDIFWNDRD 369
                                                                       
Sbjct: 330 ------------------------------------------------------------ 389

Query: 370 EENNVESNGNGNCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALTGAWGGVRPGSTHL- 429
                               GM  F R+++  F  ++ +YVWHAL G WGG+RPG+  L 
Sbjct: 390 --------------------GMGGFVREMKAAFPTVEQVYVWHALCGYWGGLRPGAPGLP 449

Query: 430 KSKITPLKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLFDSMHSYLSEAGITGVKVD 489
            +K+   + SPGL  TM DLAV K++   +GLV P +A +L++ +HS+L  +GI GVKVD
Sbjct: 450 PAKVVAPRLSPGLQRTMEDLAVDKIVNNGVGLVDPRRARELYEGLHSHLQASGIDGVKVD 509

Query: 490 VIHTLEYVSEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLLSSMQQCNDFFFLGTKQNSIG 549
           VIH LE V EEYGGRV+LAK Y+ GLT+S+ ++F G  +++SM+ CNDF  LGT+  ++G
Sbjct: 510 VIHLLEMVCEEYGGRVELAKAYFAGLTESVRRHFNGNGVIASMEHCNDFMLLGTEAVALG 569

Query: 550 RASDDFWFHDPHGDPMGMYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGS 609
           R  DDFW  DP GDP G +WLQG HMVHCAYNS+WMG  I PDWDMFQS H CA FHA S
Sbjct: 570 RVGDDFWCTDPSGDPDGTFWLQGCHMVHCAYNSLWMGAFIHPDWDMFQSTHPCAAFHAAS 629

Query: 610 RAICGGPIYVSDSVGSHDFYLIKKLAYPDGTIPRCRHFALPTRDCLFKNPLFDNKTVLKI 669
           RA+ GGP+YVSD+VG HDF L+++LA PDGTI RC  +ALPTRDCLF +PL D KT+LKI
Sbjct: 630 RAVSGGPVYVSDAVGCHDFDLLRRLALPDGTILRCERYALPTRDCLFADPLHDGKTMLKI 689

Query: 670 WNLNMYGGVIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPEAAPMGNF 729
           WN+N + GV+GAFNCQG GW R+ RR         P++      DVEW         G  
Sbjct: 690 WNVNKFSGVLGAFNCQGGGWSREARRNMCAAGFSVPVTARASPADVEWSHGG-----GGG 749

Query: 730 VEYVVYLNQAEQILHTTPKSEPLELNLEPSTFELFNFIPLKKLDS---NIKFAPIGLTNM 789
             + VY  +A + L    + E +EL LEP T+EL    P++ + S    I FAPIGL NM
Sbjct: 750 DRFAVYFVEARK-LQLLRRDESVELTLEPFTYELLVVAPVRAIVSPELGIGFAPIGLANM 783

Query: 790 FNSSGTIQHLK-YNKNG---VELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDYDGKLS 849
            N+ G +Q  +   K+G    E+ VKG G  +AYSS  P+ C  NG + EF+++ DG ++
Sbjct: 810 LNAGGAVQGFEAARKDGDVAAEVAVKGAGEMVAYSSARPRLCKVNGQDAEFKYE-DGIVT 783

BLAST of Cp4.1LG12g06330 vs. NCBI nr
Match: XP_023549394.1 (stachyose synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1784 bits (4620), Expect = 0.0
Identity = 849/849 (100.00%), Postives = 849/849 (100.00%), Query Frame = 0

Query: 1   MTSLDNYVHLSNGNISVQGVPLLSDVPSNVFFSPFSSVRSSDAPLPLFQRVHALSHKGGF 60
           MTSLDNYVHLSNGNISVQGVPLLSDVPSNVFFSPFSSVRSSDAPLPLFQRVHALSHKGGF
Sbjct: 1   MTSLDNYVHLSNGNISVQGVPLLSDVPSNVFFSPFSSVRSSDAPLPLFQRVHALSHKGGF 60

Query: 61  LGFDQTQSQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVP 120
           LGFDQTQSQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVP
Sbjct: 61  LGFDQTQSQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVP 120

Query: 121 EIRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVCDNPYNLM 180
           EIRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVCDNPYNLM
Sbjct: 121 EIRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVCDNPYNLM 180

Query: 181 KEAYATIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGVNDFFEGGVSL 240
           KEAYATIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGVNDFFEGGVSL
Sbjct: 181 KEAYATIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGVNDFFEGGVSL 240

Query: 241 RFLIIDDGWQSINIDGEDPHRDMKYIVLGANQMTARLYKFEECDKFEKYKGGTMLGPNAP 300
           RFLIIDDGWQSINIDGEDPHRDMKYIVLGANQMTARLYKFEECDKFEKYKGGTMLGPNAP
Sbjct: 241 RFLIIDDGWQSINIDGEDPHRDMKYIVLGANQMTARLYKFEECDKFEKYKGGTMLGPNAP 300

Query: 301 PFDPKKPKLLISKSIEIDNAEKNRDNAIQSGVNDVFKFETEVKKLKQELIDIFWNDRDEE 360
           PFDPKKPKLLISKSIEIDNAEKNRDNAIQSGVNDVFKFETEVKKLKQELIDIFWNDRDEE
Sbjct: 301 PFDPKKPKLLISKSIEIDNAEKNRDNAIQSGVNDVFKFETEVKKLKQELIDIFWNDRDEE 360

Query: 361 NNVESNGNGNCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALTGAWGGVRPGSTHLKSK 420
           NNVESNGNGNCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALTGAWGGVRPGSTHLKSK
Sbjct: 361 NNVESNGNGNCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALTGAWGGVRPGSTHLKSK 420

Query: 421 ITPLKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLFDSMHSYLSEAGITGVKVDVIH 480
           ITPLKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLFDSMHSYLSEAGITGVKVDVIH
Sbjct: 421 ITPLKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLFDSMHSYLSEAGITGVKVDVIH 480

Query: 481 TLEYVSEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLLSSMQQCNDFFFLGTKQNSIGRAS 540
           TLEYVSEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLLSSMQQCNDFFFLGTKQNSIGRAS
Sbjct: 481 TLEYVSEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLLSSMQQCNDFFFLGTKQNSIGRAS 540

Query: 541 DDFWFHDPHGDPMGMYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGSRAI 600
           DDFWFHDPHGDPMGMYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGSRAI
Sbjct: 541 DDFWFHDPHGDPMGMYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGSRAI 600

Query: 601 CGGPIYVSDSVGSHDFYLIKKLAYPDGTIPRCRHFALPTRDCLFKNPLFDNKTVLKIWNL 660
           CGGPIYVSDSVGSHDFYLIKKLAYPDGTIPRCRHFALPTRDCLFKNPLFDNKTVLKIWNL
Sbjct: 601 CGGPIYVSDSVGSHDFYLIKKLAYPDGTIPRCRHFALPTRDCLFKNPLFDNKTVLKIWNL 660

Query: 661 NMYGGVIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPEAAPMGNFVEY 720
           NMYGGVIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPEAAPMGNFVEY
Sbjct: 661 NMYGGVIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPEAAPMGNFVEY 720

Query: 721 VVYLNQAEQILHTTPKSEPLELNLEPSTFELFNFIPLKKLDSNIKFAPIGLTNMFNSSGT 780
           VVYLNQAEQILHTTPKSEPLELNLEPSTFELFNFIPLKKLDSNIKFAPIGLTNMFNSSGT
Sbjct: 721 VVYLNQAEQILHTTPKSEPLELNLEPSTFELFNFIPLKKLDSNIKFAPIGLTNMFNSSGT 780

Query: 781 IQHLKYNKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDYDGKLSFDLCWTEEAG 840
           IQHLKYNKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDYDGKLSFDLCWTEEAG
Sbjct: 781 IQHLKYNKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDYDGKLSFDLCWTEEAG 840

Query: 841 GFSNLDIFF 849
           GFSNLDIFF
Sbjct: 841 GFSNLDIFF 849

BLAST of Cp4.1LG12g06330 vs. NCBI nr
Match: XP_022991280.1 (stachyose synthase-like [Cucurbita maxima])

HSP 1 Score: 1651 bits (4276), Expect = 0.0
Identity = 787/850 (92.59%), Postives = 813/850 (95.65%), Query Frame = 0

Query: 1   MTSLDNYVHLSNGNISVQGVPLLSDVPSNVFFSPFSSVRSSDAPLPLFQRVHALSHKGGF 60
           MT LDNYVHLSNGNI V+GVPLLS+VPSNVFF+PFSSVRSSD PLPL QRV ALSHKGGF
Sbjct: 1   MTRLDNYVHLSNGNIDVKGVPLLSEVPSNVFFTPFSSVRSSDVPLPLLQRVRALSHKGGF 60

Query: 61  LGFDQTQSQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVP 120
           LGFDQTQSQPSDRLINSLGKFE REFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVP
Sbjct: 61  LGFDQTQSQPSDRLINSLGKFESREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVP 120

Query: 121 EIRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVCDNPYNLM 180
           EIRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGST VKASSFDAIAYIHV DNPYNLM
Sbjct: 121 EIRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTHVKASSFDAIAYIHVSDNPYNLM 180

Query: 181 KEAYATIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGVNDFFEGGVSL 240
           KEAYA IRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVW+GVNDF EGGVSL
Sbjct: 181 KEAYAAIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWHGVNDFTEGGVSL 240

Query: 241 RFLIIDDGWQSINIDGEDPHRDMKYIVLGANQMTARLYKFEECDKFEKYKGGTMLGPNAP 300
           RFLIIDDGWQSIN DGEDPHRDMK+IVLGANQMTARLYKFEECDKF KYKGGTMLGPNAP
Sbjct: 241 RFLIIDDGWQSINADGEDPHRDMKHIVLGANQMTARLYKFEECDKFGKYKGGTMLGPNAP 300

Query: 301 PFDPKKPKLLISKSIEIDNAEKNRDNAIQSGVNDVFKFETEVKKLKQELIDIFWNDRDEE 360
           PFDPKKPKLLISKSIEIDNAEK+RDN IQSGV D+ KFE +V+KLKQEL+DIF ND+DEE
Sbjct: 301 PFDPKKPKLLISKSIEIDNAEKDRDNVIQSGVTDISKFEAKVQKLKQELMDIFGNDQDEE 360

Query: 361 NNVESNGNGNCCYKG-DDMGMKAFTRDLRTKFKGLDDIYVWHALTGAWGGVRPGSTHLKS 420
              E+N N NCCYKG DDMGMKAFTRDLRTKFKGLDDIYVWHAL GAWGGVRPGSTHLKS
Sbjct: 361 ---ENNRNENCCYKGGDDMGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPGSTHLKS 420

Query: 421 KITPLKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLFDSMHSYLSEAGITGVKVDVI 480
           KITPLKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDL+DSMHSYLS+AGITGVKVDVI
Sbjct: 421 KITPLKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLYDSMHSYLSKAGITGVKVDVI 480

Query: 481 HTLEYVSEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLLSSMQQCNDFFFLGTKQNSIGRA 540
           HTLEYVSEEYGGRVDLAKTYYKGLTDSI+KNFKGTNL+SSMQQCNDFFFLGTKQNSIGRA
Sbjct: 481 HTLEYVSEEYGGRVDLAKTYYKGLTDSIMKNFKGTNLISSMQQCNDFFFLGTKQNSIGRA 540

Query: 541 SDDFWFHDPHGDPMGMYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGSRA 600
           SDDFWFHDPHGDPMG +WLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGSRA
Sbjct: 541 SDDFWFHDPHGDPMGEFWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGSRA 600

Query: 601 ICGGPIYVSDSVGSHDFYLIKKLAYPDGTIPRCRHFALPTRDCLFKNPLFDNKTVLKIWN 660
           +CGGPIYVSDSVGSHDF LIKKL YPDGTIPRC+HFALPTRDCLFKNPLFDNKTVLKIWN
Sbjct: 601 VCGGPIYVSDSVGSHDFDLIKKLVYPDGTIPRCKHFALPTRDCLFKNPLFDNKTVLKIWN 660

Query: 661 LNMYGGVIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPEAAPMGNFVE 720
           LN YGGVIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPE APMGNFVE
Sbjct: 661 LNKYGGVIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPETAPMGNFVE 720

Query: 721 YVVYLNQAEQILHTTPKSEPLELNLEPSTFELFNFIPLKKLDSNIKFAPIGLTNMFNSSG 780
           YVVYLNQA+QIL+TTP SEPLEL L+PSTFELFNFIPL+KL SNIKFAPIGLTNMFNSSG
Sbjct: 721 YVVYLNQAKQILYTTPNSEPLELTLQPSTFELFNFIPLEKLGSNIKFAPIGLTNMFNSSG 780

Query: 781 TIQHLKYNKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDYDGKLSFDLCWTEEA 840
           TIQHLKYN+N VELKVKGGGNFLAYSSGSPKKC+SNG+EV FEWDYDGKLSFDLCW EEA
Sbjct: 781 TIQHLKYNENDVELKVKGGGNFLAYSSGSPKKCVSNGMEVAFEWDYDGKLSFDLCWIEEA 840

Query: 841 GGFSNLDIFF 849
           GG SNLDI F
Sbjct: 841 GGVSNLDILF 847

BLAST of Cp4.1LG12g06330 vs. NCBI nr
Match: KAG7014305.1 (Stachyose synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1649 bits (4269), Expect = 0.0
Identity = 788/848 (92.92%), Postives = 815/848 (96.11%), Query Frame = 0

Query: 3   SLDNYVHLSNGNISVQGVPLLSDVPSNVFFSPFSSVRSSDAPLPLFQRVHALSHKGGFLG 62
           SLDNYVHLSNGNISV+GVPLLSDV SNVFFSPFSSVRSSDAPLPL QRVHALSHKGGFLG
Sbjct: 9   SLDNYVHLSNGNISVKGVPLLSDVSSNVFFSPFSSVRSSDAPLPLLQRVHALSHKGGFLG 68

Query: 63  FDQTQSQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPEI 122
           FDQ+QSQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQW+ILNV EI
Sbjct: 69  FDQSQSQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWIILNVHEI 128

Query: 123 RSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVCDNPYNLMKE 182
            SYVVIIPIIEGSFRSALHPG DGHVMIWAESGST VKASSFDAIAYIHV DNPYNLMKE
Sbjct: 129 SSYVVIIPIIEGSFRSALHPGIDGHVMIWAESGSTHVKASSFDAIAYIHVSDNPYNLMKE 188

Query: 183 AYATIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGVNDFFEGGVSLRF 242
           AYA IRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGVNDF EGG+SLRF
Sbjct: 189 AYAAIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGVNDFTEGGISLRF 248

Query: 243 LIIDDGWQSINIDGEDPHRDMKYIVLGANQMTARLYKFEECDKFEKYKGGTMLGPNAPPF 302
           LIIDDGWQSIN+DGEDPHRDMKYIVLG+NQMTARLYKFEECDKF KYKGGTMLGPNAPPF
Sbjct: 249 LIIDDGWQSINVDGEDPHRDMKYIVLGSNQMTARLYKFEECDKFGKYKGGTMLGPNAPPF 308

Query: 303 DPKKPKLLISKSIEIDNAEKNRDNAIQSGVNDVFKFETEVKKLKQELIDIFWNDRDEE-N 362
           DPKKPKLLISKSIEIDNAEK+RDNAIQSGV D+ KFE +V+KLKQELIDIF ND+DEE +
Sbjct: 309 DPKKPKLLISKSIEIDNAEKDRDNAIQSGVTDISKFEAKVQKLKQELIDIFGNDQDEEES 368

Query: 363 NVESNGNGNCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALTGAWGGVRPGSTHLKSKI 422
           +VESNGNGN  YK D++GMKAFTRDLR+KFKGLDDIYVWHAL GAWGGVRPGSTHL+SKI
Sbjct: 369 SVESNGNGNGSYKYDNIGMKAFTRDLRSKFKGLDDIYVWHALAGAWGGVRPGSTHLRSKI 428

Query: 423 TPLKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLFDSMHSYLSEAGITGVKVDVIHT 482
           T LKSSPGLDGTMTDLAVVKVIEGKI LVHPDQAYDL+DSMHSYLSEAGITGVKVDVIHT
Sbjct: 429 TSLKSSPGLDGTMTDLAVVKVIEGKIALVHPDQAYDLYDSMHSYLSEAGITGVKVDVIHT 488

Query: 483 LEYVSEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLLSSMQQCNDFFFLGTKQNSIGRASD 542
           LEYVSEEYGGRVDLAKTYYKGLTDSIVKNFKGTNL+SSMQQCNDFFFLGTKQNSIGRASD
Sbjct: 489 LEYVSEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLISSMQQCNDFFFLGTKQNSIGRASD 548

Query: 543 DFWFHDPHGDPMGMYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGSRAIC 602
           DFWFHDPHGDPMG YWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGSRAIC
Sbjct: 549 DFWFHDPHGDPMGEYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGSRAIC 608

Query: 603 GGPIYVSDSVGSHDFYLIKKLAYPDGTIPRCRHFALPTRDCLFKNPLFDNKTVLKIWNLN 662
           GGPIYVSDSVG HDF L+K+L YPDGTIPRC++FALPTRDCLFKNPLFDNKT+LKIWNLN
Sbjct: 609 GGPIYVSDSVGCHDFDLMKQLVYPDGTIPRCQYFALPTRDCLFKNPLFDNKTILKIWNLN 668

Query: 663 MYGGVIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPEAAPMGNFVEYV 722
            YGGVIGAFNCQGAGWD KE+RIKG PECYKPMSTTVHVNDVEWDQKPEAAPMGNFVEYV
Sbjct: 669 KYGGVIGAFNCQGAGWDPKEQRIKGRPECYKPMSTTVHVNDVEWDQKPEAAPMGNFVEYV 728

Query: 723 VYLNQAEQILHTTPKSEPLELNLEPSTFELFNFIPLKKLDSNIKFAPIGLTNMFNSSGTI 782
           VYLNQAEQILHTTPKSEPLEL L+PSTFELFNFIPL+KL SNIKFAPIGLTNMFNSSGTI
Sbjct: 729 VYLNQAEQILHTTPKSEPLELTLQPSTFELFNFIPLEKLGSNIKFAPIGLTNMFNSSGTI 788

Query: 783 QHLKYNKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDYDGKLSFDLCWTEEAGG 842
           QHLKYNKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDYDGKLSFDL W EEAGG
Sbjct: 789 QHLKYNKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDYDGKLSFDLPWIEEAGG 848

Query: 843 FSNLDIFF 849
            SNLDIFF
Sbjct: 849 VSNLDIFF 856

BLAST of Cp4.1LG12g06330 vs. NCBI nr
Match: XP_023547476.1 (stachyose synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1622 bits (4199), Expect = 0.0
Identity = 768/844 (91.00%), Postives = 803/844 (95.14%), Query Frame = 0

Query: 6   NYVHLSNGNISVQGVPLLSDVPSNVFFSPFSSVRSSDAPLPLFQRVHALSHKGGFLGFDQ 65
           NY  LSNGNI+V+GVPLL DVPSNVFFSPFSSVRSSDAPLPL QRVHALSHKGGFLGFDQ
Sbjct: 4   NYFDLSNGNINVKGVPLLLDVPSNVFFSPFSSVRSSDAPLPLLQRVHALSHKGGFLGFDQ 63

Query: 66  TQSQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPEIRSY 125
           +QSQPSDRLINSLGKFEGRE +SIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPE+RSY
Sbjct: 64  SQSQPSDRLINSLGKFEGRESLSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPELRSY 123

Query: 126 VVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVCDNPYNLMKEAYA 185
           VVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKAS FDAIAYIHVCDNPYNLMKEAYA
Sbjct: 124 VVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASRFDAIAYIHVCDNPYNLMKEAYA 183

Query: 186 TIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGVNDFFEGGVSLRFLII 245
            IRVHLNTFRLLEEKSVPDLV+RFGWCTWNAFFLNVNPVGVW+GVNDF EGGVSLRFLII
Sbjct: 184 AIRVHLNTFRLLEEKSVPDLVNRFGWCTWNAFFLNVNPVGVWHGVNDFTEGGVSLRFLII 243

Query: 246 DDGWQSINIDGEDPHRDMKYIVLGANQMTARLYKFEECDKFEKYKGGTMLGPNAPPFDPK 305
           DDGWQSIN DGEDPHRDMK+IVLG+NQMTARLYKFEECDKF KYKGGTMLGPNAPPFDPK
Sbjct: 244 DDGWQSINADGEDPHRDMKHIVLGSNQMTARLYKFEECDKFGKYKGGTMLGPNAPPFDPK 303

Query: 306 KPKLLISKSIEIDNAEKNRDNAIQSGVNDVFKFETEVKKLKQELIDIFWNDRDEENNVES 365
           KPKLLISKSIEIDNAEK+RDNAIQSGV DV K E +++KLKQEL+DIF ND+DEE   E+
Sbjct: 304 KPKLLISKSIEIDNAEKDRDNAIQSGVTDVSKLEAKIQKLKQELMDIFGNDQDEE---EN 363

Query: 366 NGNGNCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALTGAWGGVRPGSTHLKSKITPLK 425
           N NGN CYKGDDMGMKAFTRDLRTKFKGLDDIYVWHAL GAWGGVRPGSTHLKSKITPLK
Sbjct: 364 NRNGNRCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPGSTHLKSKITPLK 423

Query: 426 SSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLFDSMHSYLSEAGITGVKVDVIHTLEYV 485
           SSPGLDGTMTDLAVVKVIEGKI LVHPDQAYDL+DSMHSYLS+AGITGVKVDVIHTLEYV
Sbjct: 424 SSPGLDGTMTDLAVVKVIEGKIALVHPDQAYDLYDSMHSYLSKAGITGVKVDVIHTLEYV 483

Query: 486 SEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLLSSMQQCNDFFFLGTKQNSIGRASDDFWF 545
           +EEYGGRVDLAK YYKGLTDSIVKNFKGTNL+SSMQQCNDFFFLGTKQNSIGR SDDFWF
Sbjct: 484 AEEYGGRVDLAKVYYKGLTDSIVKNFKGTNLISSMQQCNDFFFLGTKQNSIGRVSDDFWF 543

Query: 546 HDPHGDPMGMYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGSRAICGGPI 605
           HDPHGDP G+YWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDH+CAKFHAGSRAICGGP+
Sbjct: 544 HDPHGDPTGVYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHMCAKFHAGSRAICGGPV 603

Query: 606 YVSDSVGSHDFYLIKKLAYPDGTIPRCRHFALPTRDCLFKNPLFDNKTVLKIWNLNMYGG 665
           YVSDSVG HDF LIKKLAYPDGTIPRC+HFALPTRDCLFKNPLFDNKTVLKIWNLN YGG
Sbjct: 604 YVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTVLKIWNLNKYGG 663

Query: 666 VIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPEAAPMGNFVEYVVYLN 725
           +IGAFNCQGAGWDRKE+RIKGHPECYKP+STTVHVNDVEWDQKPE APMGN VEY+VYLN
Sbjct: 664 IIGAFNCQGAGWDRKEKRIKGHPECYKPISTTVHVNDVEWDQKPETAPMGNSVEYIVYLN 723

Query: 726 QAEQILHTTPKSEPLELNLEPSTFELFNFIPLKKLDSNIKFAPIGLTNMFNSSGTIQHLK 785
           QAE+ILHTTPKS+PLEL L+PSTFELFNFIPLKKLDSN+KFAPIGLTNMFNSSGTIQHLK
Sbjct: 724 QAEEILHTTPKSKPLELTLQPSTFELFNFIPLKKLDSNLKFAPIGLTNMFNSSGTIQHLK 783

Query: 786 YNKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDYDGKLSFDLCWTEEAGGFSNL 845
           YN+NG ELKVKGGGNFLAYSS SPKKC+SNG+EVEFEWD  GKLS DL W EE GG SNL
Sbjct: 784 YNENGAELKVKGGGNFLAYSSRSPKKCISNGMEVEFEWDSKGKLSIDLPWIEEVGGVSNL 843

Query: 846 DIFF 849
           DI F
Sbjct: 844 DIVF 844

BLAST of Cp4.1LG12g06330 vs. NCBI nr
Match: XP_022991745.1 (stachyose synthase-like [Cucurbita maxima])

HSP 1 Score: 1621 bits (4197), Expect = 0.0
Identity = 767/844 (90.88%), Postives = 806/844 (95.50%), Query Frame = 0

Query: 6   NYVHLSNGNISVQGVPLLSDVPSNVFFSPFSSVRSSDAPLPLFQRVHALSHKGGFLGFDQ 65
           NY  LSNGNI+V+GVPLLS+VPSNVFF+PFS VRSSDAP+PL QRVHALSHKGGFLGFDQ
Sbjct: 4   NYFDLSNGNINVKGVPLLSEVPSNVFFTPFSLVRSSDAPVPLLQRVHALSHKGGFLGFDQ 63

Query: 66  TQSQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPEIRSY 125
           +QSQPSDRLINSLGKF+GRE +SIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPEIRSY
Sbjct: 64  SQSQPSDRLINSLGKFKGRESLSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPEIRSY 123

Query: 126 VVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVCDNPYNLMKEAYA 185
           VVIIPIIEGSFRSALHPGTDGHVMIWAESGST VKASSFDAIAYIHV DNPYNLMKEAYA
Sbjct: 124 VVIIPIIEGSFRSALHPGTDGHVMIWAESGSTHVKASSFDAIAYIHVSDNPYNLMKEAYA 183

Query: 186 TIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGVNDFFEGGVSLRFLII 245
            IRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVW+GVNDF EGGVSLRFLII
Sbjct: 184 AIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWHGVNDFTEGGVSLRFLII 243

Query: 246 DDGWQSINIDGEDPHRDMKYIVLGANQMTARLYKFEECDKFEKYKGGTMLGPNAPPFDPK 305
           DDGWQSIN+DGEDPHRDMK+IVLGANQMTARLYKFEECDKF KYKGGTMLGPNAPPFDPK
Sbjct: 244 DDGWQSINVDGEDPHRDMKHIVLGANQMTARLYKFEECDKFGKYKGGTMLGPNAPPFDPK 303

Query: 306 KPKLLISKSIEIDNAEKNRDNAIQSGVNDVFKFETEVKKLKQELIDIFWNDRDEENNVES 365
           KPKLLISKSIEIDNAEK+RDNAIQSGV D+ KFE +V+KLKQEL+DIF ND+DEE   E+
Sbjct: 304 KPKLLISKSIEIDNAEKDRDNAIQSGVTDISKFEAKVQKLKQELMDIFGNDQDEE---EN 363

Query: 366 NGNGNCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALTGAWGGVRPGSTHLKSKITPLK 425
           N N NCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHAL GAWGGVRPGSTHLKSKITPLK
Sbjct: 364 NRNENCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPGSTHLKSKITPLK 423

Query: 426 SSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLFDSMHSYLSEAGITGVKVDVIHTLEYV 485
           SSPGLDGTMTDLAVVKVIEGKI LVHPDQAYDL+DSMHSYLS+AGITGVKVDVIHTLEYV
Sbjct: 424 SSPGLDGTMTDLAVVKVIEGKIALVHPDQAYDLYDSMHSYLSKAGITGVKVDVIHTLEYV 483

Query: 486 SEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLLSSMQQCNDFFFLGTKQNSIGRASDDFWF 545
           +EEYGGRVDLAK YYKGLTDSIVKNFKGTNL+SSMQQCNDFFFLGTKQNSIGR SDDFWF
Sbjct: 484 AEEYGGRVDLAKVYYKGLTDSIVKNFKGTNLISSMQQCNDFFFLGTKQNSIGRVSDDFWF 543

Query: 546 HDPHGDPMGMYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGSRAICGGPI 605
           HDPHGDP G+YWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDH+CAKFHAGSRAICGGP+
Sbjct: 544 HDPHGDPTGVYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHMCAKFHAGSRAICGGPV 603

Query: 606 YVSDSVGSHDFYLIKKLAYPDGTIPRCRHFALPTRDCLFKNPLFDNKTVLKIWNLNMYGG 665
           YVSDSVG HDF LIKKLAYPDGTIPRC+HFALPTRDCLFKNPLFDNKT+LKIWNLN YGG
Sbjct: 604 YVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTILKIWNLNKYGG 663

Query: 666 VIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPEAAPMGNFVEYVVYLN 725
           +IGAFNCQGAGW+RKE+RIKG+PECYKP+STTVHVNDVEWDQKPE APMGNFVEY+VYLN
Sbjct: 664 IIGAFNCQGAGWNRKEKRIKGYPECYKPISTTVHVNDVEWDQKPETAPMGNFVEYIVYLN 723

Query: 726 QAEQILHTTPKSEPLELNLEPSTFELFNFIPLKKLDSNIKFAPIGLTNMFNSSGTIQHLK 785
           QAE+ILHTT KS+PLEL L+PSTFELFNFIPLKKLDSNIKFAPIGLTNMFNSSGTIQHLK
Sbjct: 724 QAEKILHTTLKSKPLELTLQPSTFELFNFIPLKKLDSNIKFAPIGLTNMFNSSGTIQHLK 783

Query: 786 YNKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDYDGKLSFDLCWTEEAGGFSNL 845
           YN+NGVELKVKG GNFLAYSSGSPKKC+SNG+EVEFEWD  GKLSFDL W EE GG SNL
Sbjct: 784 YNENGVELKVKGEGNFLAYSSGSPKKCISNGMEVEFEWDSKGKLSFDLPWMEEVGGVSNL 843

Query: 846 DIFF 849
           DI F
Sbjct: 844 DILF 844

BLAST of Cp4.1LG12g06330 vs. ExPASy TrEMBL
Match: A0A6J1JSF9 (stachyose synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111487982 PE=3 SV=1)

HSP 1 Score: 1651 bits (4276), Expect = 0.0
Identity = 787/850 (92.59%), Postives = 813/850 (95.65%), Query Frame = 0

Query: 1   MTSLDNYVHLSNGNISVQGVPLLSDVPSNVFFSPFSSVRSSDAPLPLFQRVHALSHKGGF 60
           MT LDNYVHLSNGNI V+GVPLLS+VPSNVFF+PFSSVRSSD PLPL QRV ALSHKGGF
Sbjct: 1   MTRLDNYVHLSNGNIDVKGVPLLSEVPSNVFFTPFSSVRSSDVPLPLLQRVRALSHKGGF 60

Query: 61  LGFDQTQSQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVP 120
           LGFDQTQSQPSDRLINSLGKFE REFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVP
Sbjct: 61  LGFDQTQSQPSDRLINSLGKFESREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVP 120

Query: 121 EIRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVCDNPYNLM 180
           EIRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGST VKASSFDAIAYIHV DNPYNLM
Sbjct: 121 EIRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTHVKASSFDAIAYIHVSDNPYNLM 180

Query: 181 KEAYATIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGVNDFFEGGVSL 240
           KEAYA IRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVW+GVNDF EGGVSL
Sbjct: 181 KEAYAAIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWHGVNDFTEGGVSL 240

Query: 241 RFLIIDDGWQSINIDGEDPHRDMKYIVLGANQMTARLYKFEECDKFEKYKGGTMLGPNAP 300
           RFLIIDDGWQSIN DGEDPHRDMK+IVLGANQMTARLYKFEECDKF KYKGGTMLGPNAP
Sbjct: 241 RFLIIDDGWQSINADGEDPHRDMKHIVLGANQMTARLYKFEECDKFGKYKGGTMLGPNAP 300

Query: 301 PFDPKKPKLLISKSIEIDNAEKNRDNAIQSGVNDVFKFETEVKKLKQELIDIFWNDRDEE 360
           PFDPKKPKLLISKSIEIDNAEK+RDN IQSGV D+ KFE +V+KLKQEL+DIF ND+DEE
Sbjct: 301 PFDPKKPKLLISKSIEIDNAEKDRDNVIQSGVTDISKFEAKVQKLKQELMDIFGNDQDEE 360

Query: 361 NNVESNGNGNCCYKG-DDMGMKAFTRDLRTKFKGLDDIYVWHALTGAWGGVRPGSTHLKS 420
              E+N N NCCYKG DDMGMKAFTRDLRTKFKGLDDIYVWHAL GAWGGVRPGSTHLKS
Sbjct: 361 ---ENNRNENCCYKGGDDMGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPGSTHLKS 420

Query: 421 KITPLKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLFDSMHSYLSEAGITGVKVDVI 480
           KITPLKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDL+DSMHSYLS+AGITGVKVDVI
Sbjct: 421 KITPLKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLYDSMHSYLSKAGITGVKVDVI 480

Query: 481 HTLEYVSEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLLSSMQQCNDFFFLGTKQNSIGRA 540
           HTLEYVSEEYGGRVDLAKTYYKGLTDSI+KNFKGTNL+SSMQQCNDFFFLGTKQNSIGRA
Sbjct: 481 HTLEYVSEEYGGRVDLAKTYYKGLTDSIMKNFKGTNLISSMQQCNDFFFLGTKQNSIGRA 540

Query: 541 SDDFWFHDPHGDPMGMYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGSRA 600
           SDDFWFHDPHGDPMG +WLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGSRA
Sbjct: 541 SDDFWFHDPHGDPMGEFWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGSRA 600

Query: 601 ICGGPIYVSDSVGSHDFYLIKKLAYPDGTIPRCRHFALPTRDCLFKNPLFDNKTVLKIWN 660
           +CGGPIYVSDSVGSHDF LIKKL YPDGTIPRC+HFALPTRDCLFKNPLFDNKTVLKIWN
Sbjct: 601 VCGGPIYVSDSVGSHDFDLIKKLVYPDGTIPRCKHFALPTRDCLFKNPLFDNKTVLKIWN 660

Query: 661 LNMYGGVIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPEAAPMGNFVE 720
           LN YGGVIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPE APMGNFVE
Sbjct: 661 LNKYGGVIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPETAPMGNFVE 720

Query: 721 YVVYLNQAEQILHTTPKSEPLELNLEPSTFELFNFIPLKKLDSNIKFAPIGLTNMFNSSG 780
           YVVYLNQA+QIL+TTP SEPLEL L+PSTFELFNFIPL+KL SNIKFAPIGLTNMFNSSG
Sbjct: 721 YVVYLNQAKQILYTTPNSEPLELTLQPSTFELFNFIPLEKLGSNIKFAPIGLTNMFNSSG 780

Query: 781 TIQHLKYNKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDYDGKLSFDLCWTEEA 840
           TIQHLKYN+N VELKVKGGGNFLAYSSGSPKKC+SNG+EV FEWDYDGKLSFDLCW EEA
Sbjct: 781 TIQHLKYNENDVELKVKGGGNFLAYSSGSPKKCVSNGMEVAFEWDYDGKLSFDLCWIEEA 840

Query: 841 GGFSNLDIFF 849
           GG SNLDI F
Sbjct: 841 GGVSNLDILF 847

BLAST of Cp4.1LG12g06330 vs. ExPASy TrEMBL
Match: A0A6J1JTU2 (stachyose synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111488278 PE=3 SV=1)

HSP 1 Score: 1621 bits (4197), Expect = 0.0
Identity = 767/844 (90.88%), Postives = 806/844 (95.50%), Query Frame = 0

Query: 6   NYVHLSNGNISVQGVPLLSDVPSNVFFSPFSSVRSSDAPLPLFQRVHALSHKGGFLGFDQ 65
           NY  LSNGNI+V+GVPLLS+VPSNVFF+PFS VRSSDAP+PL QRVHALSHKGGFLGFDQ
Sbjct: 4   NYFDLSNGNINVKGVPLLSEVPSNVFFTPFSLVRSSDAPVPLLQRVHALSHKGGFLGFDQ 63

Query: 66  TQSQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPEIRSY 125
           +QSQPSDRLINSLGKF+GRE +SIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPEIRSY
Sbjct: 64  SQSQPSDRLINSLGKFKGRESLSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPEIRSY 123

Query: 126 VVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVCDNPYNLMKEAYA 185
           VVIIPIIEGSFRSALHPGTDGHVMIWAESGST VKASSFDAIAYIHV DNPYNLMKEAYA
Sbjct: 124 VVIIPIIEGSFRSALHPGTDGHVMIWAESGSTHVKASSFDAIAYIHVSDNPYNLMKEAYA 183

Query: 186 TIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGVNDFFEGGVSLRFLII 245
            IRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVW+GVNDF EGGVSLRFLII
Sbjct: 184 AIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWHGVNDFTEGGVSLRFLII 243

Query: 246 DDGWQSINIDGEDPHRDMKYIVLGANQMTARLYKFEECDKFEKYKGGTMLGPNAPPFDPK 305
           DDGWQSIN+DGEDPHRDMK+IVLGANQMTARLYKFEECDKF KYKGGTMLGPNAPPFDPK
Sbjct: 244 DDGWQSINVDGEDPHRDMKHIVLGANQMTARLYKFEECDKFGKYKGGTMLGPNAPPFDPK 303

Query: 306 KPKLLISKSIEIDNAEKNRDNAIQSGVNDVFKFETEVKKLKQELIDIFWNDRDEENNVES 365
           KPKLLISKSIEIDNAEK+RDNAIQSGV D+ KFE +V+KLKQEL+DIF ND+DEE   E+
Sbjct: 304 KPKLLISKSIEIDNAEKDRDNAIQSGVTDISKFEAKVQKLKQELMDIFGNDQDEE---EN 363

Query: 366 NGNGNCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALTGAWGGVRPGSTHLKSKITPLK 425
           N N NCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHAL GAWGGVRPGSTHLKSKITPLK
Sbjct: 364 NRNENCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPGSTHLKSKITPLK 423

Query: 426 SSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLFDSMHSYLSEAGITGVKVDVIHTLEYV 485
           SSPGLDGTMTDLAVVKVIEGKI LVHPDQAYDL+DSMHSYLS+AGITGVKVDVIHTLEYV
Sbjct: 424 SSPGLDGTMTDLAVVKVIEGKIALVHPDQAYDLYDSMHSYLSKAGITGVKVDVIHTLEYV 483

Query: 486 SEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLLSSMQQCNDFFFLGTKQNSIGRASDDFWF 545
           +EEYGGRVDLAK YYKGLTDSIVKNFKGTNL+SSMQQCNDFFFLGTKQNSIGR SDDFWF
Sbjct: 484 AEEYGGRVDLAKVYYKGLTDSIVKNFKGTNLISSMQQCNDFFFLGTKQNSIGRVSDDFWF 543

Query: 546 HDPHGDPMGMYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGSRAICGGPI 605
           HDPHGDP G+YWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDH+CAKFHAGSRAICGGP+
Sbjct: 544 HDPHGDPTGVYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHMCAKFHAGSRAICGGPV 603

Query: 606 YVSDSVGSHDFYLIKKLAYPDGTIPRCRHFALPTRDCLFKNPLFDNKTVLKIWNLNMYGG 665
           YVSDSVG HDF LIKKLAYPDGTIPRC+HFALPTRDCLFKNPLFDNKT+LKIWNLN YGG
Sbjct: 604 YVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTILKIWNLNKYGG 663

Query: 666 VIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPEAAPMGNFVEYVVYLN 725
           +IGAFNCQGAGW+RKE+RIKG+PECYKP+STTVHVNDVEWDQKPE APMGNFVEY+VYLN
Sbjct: 664 IIGAFNCQGAGWNRKEKRIKGYPECYKPISTTVHVNDVEWDQKPETAPMGNFVEYIVYLN 723

Query: 726 QAEQILHTTPKSEPLELNLEPSTFELFNFIPLKKLDSNIKFAPIGLTNMFNSSGTIQHLK 785
           QAE+ILHTT KS+PLEL L+PSTFELFNFIPLKKLDSNIKFAPIGLTNMFNSSGTIQHLK
Sbjct: 724 QAEKILHTTLKSKPLELTLQPSTFELFNFIPLKKLDSNIKFAPIGLTNMFNSSGTIQHLK 783

Query: 786 YNKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDYDGKLSFDLCWTEEAGGFSNL 845
           YN+NGVELKVKG GNFLAYSSGSPKKC+SNG+EVEFEWD  GKLSFDL W EE GG SNL
Sbjct: 784 YNENGVELKVKGEGNFLAYSSGSPKKCISNGMEVEFEWDSKGKLSFDLPWMEEVGGVSNL 843

Query: 846 DIFF 849
           DI F
Sbjct: 844 DILF 844

BLAST of Cp4.1LG12g06330 vs. ExPASy TrEMBL
Match: A0A6J1GP33 (stachyose synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111455850 PE=3 SV=1)

HSP 1 Score: 1620 bits (4195), Expect = 0.0
Identity = 769/844 (91.11%), Postives = 804/844 (95.26%), Query Frame = 0

Query: 6   NYVHLSNGNISVQGVPLLSDVPSNVFFSPFSSVRSSDAPLPLFQRVHALSHKGGFLGFDQ 65
           NY  LSNGNI+V+GVPLLS+VPSNVFFSPFSSVRSSDAPLPL QRVHALSHKGGFLGFDQ
Sbjct: 4   NYFDLSNGNINVKGVPLLSEVPSNVFFSPFSSVRSSDAPLPLLQRVHALSHKGGFLGFDQ 63

Query: 66  TQSQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPEIRSY 125
           +QSQPSDRLINSLGKFEGRE +SIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPEIRSY
Sbjct: 64  SQSQPSDRLINSLGKFEGRESLSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPEIRSY 123

Query: 126 VVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVCDNPYNLMKEAYA 185
           VVI+PIIEGSFRSALHPGTDGHVMIWAESGSTQVKAS FDAIAYIHV DNPYNLMKEAYA
Sbjct: 124 VVIVPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASRFDAIAYIHVSDNPYNLMKEAYA 183

Query: 186 TIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGVNDFFEGGVSLRFLII 245
            IR+HLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVW+GVNDF EGGVSLRFLII
Sbjct: 184 AIRIHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWHGVNDFTEGGVSLRFLII 243

Query: 246 DDGWQSINIDGEDPHRDMKYIVLGANQMTARLYKFEECDKFEKYKGGTMLGPNAPPFDPK 305
           DDGWQSIN DGEDPHRDMK+IVLG+NQMTARLYKFEECDKF KYKGGTMLGPNAPPFDPK
Sbjct: 244 DDGWQSINADGEDPHRDMKHIVLGSNQMTARLYKFEECDKFGKYKGGTMLGPNAPPFDPK 303

Query: 306 KPKLLISKSIEIDNAEKNRDNAIQSGVNDVFKFETEVKKLKQELIDIFWNDRDEENNVES 365
           KPKLLISKSIEIDNAEK+RDNAIQSGV D+ KFE +V+KLKQEL+DIF ND+DEE   E+
Sbjct: 304 KPKLLISKSIEIDNAEKDRDNAIQSGVTDISKFEAKVQKLKQELMDIFGNDQDEE---EN 363

Query: 366 NGNGNCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALTGAWGGVRPGSTHLKSKITPLK 425
           NGNGN  YKGDDMGMKAFTRDLRTKFKGLDDIYVWHAL GAWGGVRPGSTHLKSKITPLK
Sbjct: 364 NGNGN--YKGDDMGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPGSTHLKSKITPLK 423

Query: 426 SSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLFDSMHSYLSEAGITGVKVDVIHTLEYV 485
           SSPGLDGTMTDLAVVKVIEGKI LVHPDQAYDL+DSMHSYLS+AGITGVKVDVIHTLEYV
Sbjct: 424 SSPGLDGTMTDLAVVKVIEGKIALVHPDQAYDLYDSMHSYLSKAGITGVKVDVIHTLEYV 483

Query: 486 SEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLLSSMQQCNDFFFLGTKQNSIGRASDDFWF 545
           +EEYGGRVDLAK YYKGLTDSIVKNFKGTNL+SSMQQCNDFFFLGTKQNSIGR SDDFWF
Sbjct: 484 AEEYGGRVDLAKVYYKGLTDSIVKNFKGTNLISSMQQCNDFFFLGTKQNSIGRVSDDFWF 543

Query: 546 HDPHGDPMGMYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGSRAICGGPI 605
           HDP GDP G+YWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDH+CAKFHAGSRAICGGP+
Sbjct: 544 HDPQGDPTGVYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHMCAKFHAGSRAICGGPV 603

Query: 606 YVSDSVGSHDFYLIKKLAYPDGTIPRCRHFALPTRDCLFKNPLFDNKTVLKIWNLNMYGG 665
           YVSDSVG HDF LIKKLAYPDGTIPRC+HFALPTRDCLFKNPLFDNKTVLKIWNLN YGG
Sbjct: 604 YVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTVLKIWNLNKYGG 663

Query: 666 VIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPEAAPMGNFVEYVVYLN 725
           +IGAFNCQGAGWDRKE+RIKGHPECYKP+STTVHVNDVEWDQKPE APMGN VEY+VYLN
Sbjct: 664 IIGAFNCQGAGWDRKEKRIKGHPECYKPISTTVHVNDVEWDQKPETAPMGNSVEYIVYLN 723

Query: 726 QAEQILHTTPKSEPLELNLEPSTFELFNFIPLKKLDSNIKFAPIGLTNMFNSSGTIQHLK 785
           QAE+ILHTTPKS+PLEL L+PSTFELFNF P+KKLDSNIKFAPIGLTNMFNSSGTIQHLK
Sbjct: 724 QAEEILHTTPKSKPLELTLQPSTFELFNFTPIKKLDSNIKFAPIGLTNMFNSSGTIQHLK 783

Query: 786 YNKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDYDGKLSFDLCWTEEAGGFSNL 845
           YNKNGVELKVKGGGNFLAYSSGSPKKC+SNG+EVEFEWD  GKLS DL W EE GG SNL
Sbjct: 784 YNKNGVELKVKGGGNFLAYSSGSPKKCISNGMEVEFEWDSKGKLSLDLPWMEEVGGVSNL 842

Query: 846 DIFF 849
           DI F
Sbjct: 844 DILF 842

BLAST of Cp4.1LG12g06330 vs. ExPASy TrEMBL
Match: A0A6J1DJ41 (stachyose synthase-like OS=Momordica charantia OX=3673 GN=LOC111020515 PE=3 SV=1)

HSP 1 Score: 1531 bits (3965), Expect = 0.0
Identity = 728/852 (85.45%), Postives = 783/852 (91.90%), Query Frame = 0

Query: 3   SLDNYVHLSNGNISVQGVPLLSDVPSNVFFSPFSSV-RSSDAPLPLFQRVHALSHKGGFL 62
           +L+NY+  S G ISV+GVPLLS+VPSNVFFSPFSS+ R+SDAPLPL QRVH LS KGGFL
Sbjct: 19  TLENYIDCSKGKISVEGVPLLSEVPSNVFFSPFSSIFRTSDAPLPLLQRVHGLSRKGGFL 78

Query: 63  GFDQTQSQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPE 122
           GF+Q Q  PSDRL+NSLGKFEGREFVS+FRFKTWWSTMW+G+SGSD+QMETQ VILNVPE
Sbjct: 79  GFEQMQ--PSDRLMNSLGKFEGREFVSVFRFKTWWSTMWIGNSGSDLQMETQLVILNVPE 138

Query: 123 IRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVCDNPYNLMK 182
           IRSYVVIIPIIEGSFRSALHPGTDGHV+IWAESGSTQVKASSF+AIAYIHV DNPYNLMK
Sbjct: 139 IRSYVVIIPIIEGSFRSALHPGTDGHVLIWAESGSTQVKASSFNAIAYIHVSDNPYNLMK 198

Query: 183 EAYATIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGVNDFFEGGVSLR 242
           EAYA IRVHLNTFRLLEEKSVP+L D+FGWCTWNAFFLNV PVGVWNGVNDF EGG+SLR
Sbjct: 199 EAYAAIRVHLNTFRLLEEKSVPNLADKFGWCTWNAFFLNVEPVGVWNGVNDFAEGGISLR 258

Query: 243 FLIIDDGWQSINIDGEDPHRDMKYIVLGANQMTARLYKFEECDKFEKYKGGTMLGPNAPP 302
           FLIIDDGWQSINIDGEDP+RDMK IVLGA+QMTARLYKFEECDKF+KYKGGT+LGPN PP
Sbjct: 259 FLIIDDGWQSINIDGEDPNRDMKNIVLGASQMTARLYKFEECDKFKKYKGGTLLGPNPPP 318

Query: 303 FDPKKPKLLISKSIEIDNAEKNRDNAIQSGVNDVFKFETEVKKLKQELIDIFWNDRDEEN 362
           FDPKKPKLLISKSIE+D AEK+RDNAIQSGV DV KFE +V+KLKQE+ID+F     EE 
Sbjct: 319 FDPKKPKLLISKSIELDRAEKDRDNAIQSGVTDVSKFEAKVQKLKQEMIDMFGK---EEG 378

Query: 363 NVESNGNGNCC----YKGDDMGMKAFTRDLRTKFKGLDDIYVWHALTGAWGGVRPGSTHL 422
             ES+   N C    Y  D+ GMKAFTRDLRTKFKGLDDIYVWHAL GAWGGVRPGSTHL
Sbjct: 379 GEESSAVSNDCESRSYTADNFGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPGSTHL 438

Query: 423 KSKITPLKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLFDSMHSYLSEAGITGVKVD 482
            SKI P K SPGLDGTMTDLAVVK++EGKIGLVHPDQAYDLFDSMHSYLS+ GITGVKVD
Sbjct: 439 SSKIIPCKPSPGLDGTMTDLAVVKIMEGKIGLVHPDQAYDLFDSMHSYLSKVGITGVKVD 498

Query: 483 VIHTLEYVSEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLLSSMQQCNDFFFLGTKQNSIG 542
           VIHTLEYVSEEYGGRVDLAK YY GLT+SI+KNFKGTNL+SSMQQCNDFFFLGTKQN+IG
Sbjct: 499 VIHTLEYVSEEYGGRVDLAKAYYNGLTNSIIKNFKGTNLISSMQQCNDFFFLGTKQNAIG 558

Query: 543 RASDDFWFHDPHGDPMGMYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGS 602
           R SDDFWFHDP GDPMG+YWLQGVHMVHCAYNSMWMGQ IQPDWDMFQSDH CAKFHAGS
Sbjct: 559 RVSDDFWFHDPQGDPMGVYWLQGVHMVHCAYNSMWMGQFIQPDWDMFQSDHQCAKFHAGS 618

Query: 603 RAICGGPIYVSDSVGSHDFYLIKKLAYPDGTIPRCRHFALPTRDCLFKNPLFDNKTVLKI 662
           RAICGGP+YVSDSVG HDF LIKKLAYPDGTIPRC+HFALPTRDCLFKNP+FDNKTVLKI
Sbjct: 619 RAICGGPVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPIFDNKTVLKI 678

Query: 663 WNLNMYGGVIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPEAAPMGNF 722
           WNLN YGG+IGAFNCQGAGWD KE+RIKGHPECY PMSTTVHVNDVEW+QKPEAAPMGNF
Sbjct: 679 WNLNKYGGIIGAFNCQGAGWDPKEQRIKGHPECYNPMSTTVHVNDVEWNQKPEAAPMGNF 738

Query: 723 VEYVVYLNQAEQILHTTPKSEPLELNLEPSTFELFNFIPLKKLDSNIKFAPIGLTNMFNS 782
           VEY+VYLNQAEQILHTTPKSEPL++ L+PSTFELF+FIPL+K+ S+IKFAPIGLTNMFNS
Sbjct: 739 VEYIVYLNQAEQILHTTPKSEPLKVTLQPSTFELFSFIPLRKIGSSIKFAPIGLTNMFNS 798

Query: 783 SGTIQHLKYNKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDYDGKLSFDLCWTE 842
           SGTIQHLKYN+NGVELKVKGGG+FLAYSSGSPKKC+SNG EVEFEWD  GKL FDL   E
Sbjct: 799 SGTIQHLKYNENGVELKVKGGGSFLAYSSGSPKKCVSNGTEVEFEWDSQGKLGFDLPGNE 858

Query: 843 EAGGFSNLDIFF 849
           EAGG SNLDIFF
Sbjct: 859 EAGGVSNLDIFF 865

BLAST of Cp4.1LG12g06330 vs. ExPASy TrEMBL
Match: A0A1S3BRI8 (stachyose synthase OS=Cucumis melo OX=3656 GN=LOC103492745 PE=3 SV=1)

HSP 1 Score: 1481 bits (3834), Expect = 0.0
Identity = 690/847 (81.46%), Postives = 771/847 (91.03%), Query Frame = 0

Query: 4   LDNYVHLSNGNISVQGVPLLSDVPSNVFFSPFSSV-RSSDAPLPLFQRVHALSHKGGFLG 63
           L+N +  S+G ISV+GVP+LS+VP+NVFFSPFSS+ +SSDAPLPL QRVH+LS+KGGFLG
Sbjct: 20  LENLIDFSDGKISVKGVPVLSEVPNNVFFSPFSSISQSSDAPLPLLQRVHSLSYKGGFLG 79

Query: 64  FDQTQSQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPEI 123
           FDQTQ  PSDRL NSLGKF+GREFVS+FRFKTWWSTMWVG+SGSD+QMETQWV+LN+PEI
Sbjct: 80  FDQTQ--PSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQWVMLNIPEI 139

Query: 124 RSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVCDNPYNLMKE 183
           +SYVVIIPIIEGSFRSALHPGTDG V+I AESGST VKASSFDAIAY+HV DNPY LMKE
Sbjct: 140 KSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFDAIAYVHVSDNPYRLMKE 199

Query: 184 AYATIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGVNDFFEGGVSLRF 243
           AYA +RVHLNTFRLLEEK V  LVD+FGWCTW+AF+L V+PVG+WNGVNDF EGG+S RF
Sbjct: 200 AYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVGIWNGVNDFVEGGISPRF 259

Query: 244 LIIDDGWQSINIDGEDPHRDMKYIVLGANQMTARLYKFEECDKFEKYKGGTMLGPNAPPF 303
           LIIDDGWQSIN+DGEDP RD K +VLG  QMTARLY F+EC+KF KYKGG+++GPNAP F
Sbjct: 260 LIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYSFDECEKFRKYKGGSLMGPNAPSF 319

Query: 304 DPKKPKLLISKSIEIDNAEKNRDNAIQSGVNDVFKFETEVKKLKQELIDIFWNDRDEENN 363
           DPKKPKLLI+K+IEI++AEK RD AI SGV +V KFET+++KLK+EL++IF N+ +EE+ 
Sbjct: 320 DPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKLKEELLEIFGNEEEEEST 379

Query: 364 VESNGNGNCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALTGAWGGVRPGSTHLKSKIT 423
             + G  +C  K D+ GMKAFTRDLRTKFKGLDDI+VWHAL GAWGGVRPG+THL SKI 
Sbjct: 380 AINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAGAWGGVRPGATHLNSKII 439

Query: 424 PLKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLFDSMHSYLSEAGITGVKVDVIHTL 483
           P K SPGLDGTMTDLAVVK+IEG IGLVHPDQA D FDSMHSYLS+ GITGVKVDV+HTL
Sbjct: 440 PCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGITGVKVDVMHTL 499

Query: 484 EYVSEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLLSSMQQCNDFFFLGTKQNSIGRASDD 543
           EYVSEEYGGRVDLAK YYKGLT+S++KNFKGT L SSMQQCNDFF+LGTKQNSIGR  DD
Sbjct: 500 EYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFSSMQQCNDFFYLGTKQNSIGRVGDD 559

Query: 544 FWFHDPHGDPMGMYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGSRAICG 603
           FWF DP+GDPMG+YWLQGVHM+HCAYNSMWMGQIIQPDWDMFQSDH+CAKFHAGSRAICG
Sbjct: 560 FWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQSDHLCAKFHAGSRAICG 619

Query: 604 GPIYVSDSVGSHDFYLIKKLAYPDGTIPRCRHFALPTRDCLFKNPLFDNKTVLKIWNLNM 663
           GP+YVSDSVG H+F LIK+L YPDGTIPRC+HFALPTRDCLFKNPLFDNKTVLKIWNLN 
Sbjct: 620 GPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFKNPLFDNKTVLKIWNLNK 679

Query: 664 YGGVIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPEAAPMGNFVEYVV 723
           YGGVIG FNCQGAGWD KE+RIKGHPECYKPMSTTVHVND+EWDQKPEAAPMGNFVEY+V
Sbjct: 680 YGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEWDQKPEAAPMGNFVEYIV 739

Query: 724 YLNQAEQILHTTPKSEPLELNLEPSTFELFNFIPLKKLDSNIKFAPIGLTNMFNSSGTIQ 783
           YLNQAEQI+HTTPKSEPL+  ++PSTFELFNFIPL+KL SNIKFAPIGLTNMFNSSGTIQ
Sbjct: 740 YLNQAEQIIHTTPKSEPLKATIQPSTFELFNFIPLRKLGSNIKFAPIGLTNMFNSSGTIQ 799

Query: 784 HLKYNKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDYDGKLSFDLCWTEEAGGF 843
           HLKYN+NGVELKVKGGGNFLAYSSGSPKKCLSNG E++F W+ DGKLSFD+ W EEAGG 
Sbjct: 800 HLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNGTELKFVWNSDGKLSFDVHWIEEAGGI 859

Query: 844 SNLDIFF 849
           SNLDIFF
Sbjct: 860 SNLDIFF 864

BLAST of Cp4.1LG12g06330 vs. TAIR 10
Match: AT4G01970.1 (stachyose synthase )

HSP 1 Score: 1041.2 bits (2691), Expect = 4.6e-304
Identity = 500/857 (58.34%), Postives = 642/857 (74.91%), Query Frame = 0

Query: 6   NYVHLSNGNISVQ-GVPLLSDVPSNVFFSPFSSVR-SSDAPLPLFQRVHALSHKGGFLGF 65
           N  +LS G++  +   P+L DVP NV F+PFSS   S+DAPLP+  RV A +HKGGFLGF
Sbjct: 32  NSFNLSEGSLCAKDSTPILFDVPQNVTFTPFSSHSISTDAPLPILLRVQANAHKGGFLGF 91

Query: 66  DQTQSQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPEIR 125
             T+  PSDRL NSLG+FE REF+S+FRFK WWST W+G SGSD+Q ETQWV+L +PEI 
Sbjct: 92  --TKESPSDRLTNSLGRFEDREFLSLFRFKMWWSTAWIGKSGSDLQAETQWVMLKIPEID 151

Query: 126 SYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVCDNPYNLMKEA 185
           SYV IIP IEG+FR++L PG  G+V+I AESGST+VK SSF +IAYIH+CDNPYNLMKEA
Sbjct: 152 SYVAIIPTIEGAFRASLTPGEKGNVLICAESGSTKVKESSFKSIAYIHICDNPYNLMKEA 211

Query: 186 YATIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGVNDFFEGGVSLRFL 245
           ++ +RVH+NTF+LLEEK +P +VD+FGWCTW+A +L V+P  +W GV +F +GGV  +F+
Sbjct: 212 FSALRVHMNTFKLLEEKKLPKIVDKFGWCTWDACYLTVDPATIWTGVKEFEDGGVCPKFV 271

Query: 246 IIDDGWQSINIDGEDPHRDMKYIVLGANQMTARLYKFEECDKFEKYKGGTMLGPNAPPFD 305
           IIDDGWQSIN DG++  +D + +VLG  QMTARL  F+EC KF  YKGG+ +  +A  F+
Sbjct: 272 IIDDGWQSINFDGDELDKDAENLVLGGEQMTARLTSFKECKKFRNYKGGSFITSDASHFN 331

Query: 306 PKKPKLLISKSIE-IDNAEKNRDNAIQSGVNDVFKFETEVKKLKQELIDIFWNDRDEENN 365
           P KPK+LI K+ E I      R    +SG  D+ + + ++K L +EL  +F    DE   
Sbjct: 332 PLKPKMLIYKATERIQAIILRRKLVKESGEQDLTELDEKIKILSEELNAMF----DEVEK 391

Query: 366 VESNGNGNCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALTGAWGGVRPGS-THLKSKI 425
            ES G+ +        GM AFT+DLR +FK LDDIYVWHAL GAW GVRP +   LK+K+
Sbjct: 392 EESLGSDDV----SGSGMAAFTKDLRLRFKSLDDIYVWHALCGAWNGVRPETMMDLKAKV 451

Query: 426 TPLKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLFDSMHSYLSEAGITGVKVDVIHT 485
            P + SP L  TM DLAV KV+E  IGLVHP +A++ +DSMHSYL+  G+TG K+DV  T
Sbjct: 452 APFELSPSLGATMADLAVDKVVEAGIGLVHPSKAHEFYDSMHSYLASVGVTGAKIDVFQT 511

Query: 486 LEYVSEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLLSSMQQCNDFFFLGTKQNSIGRASD 545
           LE ++EE+GGRV+LAK YY GLT+S++KNF GT++++SMQQCN+FFFL TKQ SIGR  D
Sbjct: 512 LESLAEEHGGRVELAKAYYDGLTESMIKNFNGTDVIASMQQCNEFFFLATKQISIGRVGD 571

Query: 546 DFWFHDPHGDPMGMYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGSRAIC 605
           DFW+ DP+GDP G+YWLQGVHM+HC+YNS+WMGQ+IQPDWDMFQSDHVCA++HA SRAIC
Sbjct: 572 DFWWQDPYGDPQGVYWLQGVHMIHCSYNSIWMGQMIQPDWDMFQSDHVCAEYHAASRAIC 631

Query: 606 GGPIYVSDSVG--SHDFYLIKKLAYPDGTIPRCRHFALPTRDCLFKNPLFDNKTVLKIWN 665
           GGP+Y+SD +G  SH+F LIKKLA+ DGTIPRC H+ALPTRD LFKNPLFD +++LKI+N
Sbjct: 632 GGPVYLSDHLGKASHNFDLIKKLAFFDGTIPRCVHYALPTRDSLFKNPLFDKESILKIFN 691

Query: 666 LNMYGGVIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPEAAPMGNFV- 725
            N +GGVIG FNCQGAGW  +E R KG+ ECY  +S TVHV+D+EWDQ PEAA  G+ V 
Sbjct: 692 FNKFGGVIGTFNCQGAGWSPEEHRFKGYKECYTTVSGTVHVSDIEWDQNPEAA--GSQVT 751

Query: 726 ---EYVVYLNQAEQILHTTPKSEPLELNLEPSTFELFNFIPLKKL-DSNIKFAPIGLTNM 785
              +Y+VY  Q+E+IL    KSE +++ LEPS F+L +F+P+ +L  S ++FAP+GL NM
Sbjct: 752 YTGDYLVYKQQSEEILFMNSKSEAMKITLEPSAFDLLSFVPVTELVSSGVRFAPLGLINM 811

Query: 786 FNSSGTIQHLKY-NKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDYD-GKLSFD 845
           FN  GT+Q +K    N + + VKG G F+AYSS +P KC  N  E EF+W+ + GKLSF 
Sbjct: 812 FNCVGTVQDMKVTGDNSIRVDVKGEGRFMAYSSSAPVKCYLNDKEAEFKWEEETGKLSFF 871

Query: 846 LCWTEEAGGFSNLDIFF 850
           + W EE+GG S+L   F
Sbjct: 872 VPWVEESGGISHLSFTF 876

BLAST of Cp4.1LG12g06330 vs. TAIR 10
Match: AT5G40390.1 (Raffinose synthase family protein )

HSP 1 Score: 718.4 bits (1853), Expect = 6.8e-207
Identity = 380/853 (44.55%), Postives = 497/853 (58.26%), Query Frame = 0

Query: 10  LSNGNISVQGVPLLSDVPSNVFF--SPFSSVRSSDAPLPLFQRVHALSHKGGFLGFDQTQ 69
           L +  +   G  +L+DVP NV    SP+  V     PL +          G F+GF+   
Sbjct: 24  LEDSTLLANGQVVLTDVPVNVTLTSSPY-LVDKDGVPLDV--------SAGSFIGFN-LD 83

Query: 70  SQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVP------- 129
            +P    + S+GK +   F+SIFRFK WW+T WVGS+G DI+ ETQ +IL+         
Sbjct: 84  GEPKSHHVASIGKLKNIRFMSIFRFKVWWTTHWVGSNGRDIENETQIIILDQSGSDSGPG 143

Query: 130 --EIRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVCDNPYN 189
               R YV+++P++EGSFRS+   G D  V +  ESGST+V  S F  I Y+H  D+P+ 
Sbjct: 144 SGSGRPYVLLLPLLEGSFRSSFQSGEDDDVAVCVESGSTEVTGSEFRQIVYVHAGDDPFK 203

Query: 190 LMKEAYATIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGVNDFFEGGV 249
           L+K+A   IRVH+NTF+LLEEKS P +VD+FGWCTW+AF+L VNP GV  GV    +GG 
Sbjct: 204 LVKDAMKVIRVHMNTFKLLEEKSPPGIVDKFGWCTWDAFYLTVNPDGVHKGVKCLVDGGC 263

Query: 250 SLRFLIIDDGWQSINIDGEDPHRDMKYIVLGANQMTARLYKFEECDKFEKYKGGTMLGPN 309
               ++IDDGWQSI  D +    +   I +   QM  RL KFEE  KF+ Y         
Sbjct: 264 PPGLVLIDDGWQSIGHDSDGIDVEGMNITVAGEQMPCRLLKFEENHKFKDY--------- 323

Query: 310 APPFDPKKPKLLISKSIEIDNAEKNRDNAIQSGVNDVFKFETEVKKLKQELIDIFWNDRD 369
                PK                                                     
Sbjct: 324 ---VSPKDQ--------------------------------------------------- 383

Query: 370 EENNVESNGNGNCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALTGAWGGVRPGSTHL- 429
                            +D+GMKAF RDL+ +F  +D IYVWHAL G WGG+RP +  L 
Sbjct: 384 -----------------NDVGMKAFVRDLKDEFSTVDYIYVWHALCGYWGGLRPEAPALP 443

Query: 430 KSKITPLKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLFDSMHSYLSEAGITGVKVD 489
            S I   + SPGL  TM DLAV K+IE  IG   PD A + ++ +HS+L  AGI GVKVD
Sbjct: 444 PSTIIRPELSPGLKLTMEDLAVDKIIETGIGFASPDLAKEFYEGLHSHLQNAGIDGVKVD 503

Query: 490 VIHTLEYVSEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLLSSMQQCNDFFFLGTKQNSIG 549
           VIH LE + ++YGGRVDLAK Y+K LT S+ K+F G  +++SM+ CNDF FLGT+  S+G
Sbjct: 504 VIHILEMLCQKYGGRVDLAKAYFKALTSSVNKHFNGNGVIASMEHCNDFMFLGTEAISLG 563

Query: 550 RASDDFWFHDPHGDPMGMYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGS 609
           R  DDFW  DP GDP G +WLQG HMVHCAYNS+WMG  IQPDWDMFQS H CA+FHA S
Sbjct: 564 RVGDDFWCTDPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIQPDWDMFQSTHPCAEFHAAS 623

Query: 610 RAICGGPIYVSDSVGSHDFYLIKKLAYPDGTIPRCRHFALPTRDCLFKNPLFDNKTVLKI 669
           RAI GGPIY+SD VG HDF L+K+L  P+G+I RC ++ALPTRD LF++PL D KT+LKI
Sbjct: 624 RAISGGPIYISDCVGKHDFDLLKRLVLPNGSILRCEYYALPTRDRLFEDPLHDGKTMLKI 683

Query: 670 WNLNMYGGVIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPEAAPMGNF 729
           WNLN Y GVIGAFNCQG GW R+ RR +   EC   ++ T    DVEW+       + N 
Sbjct: 684 WNLNKYTGVIGAFNCQGGGWCRETRRNQCFSECVNTLTATTSPKDVEWNSGSSPISIANV 743

Query: 730 VEYVVYLNQAEQILHTTPKSEPLELNLEPSTFELFNFIPLKKLDSN-IKFAPIGLTNMFN 789
            E+ ++L+Q++++L  +  ++ LEL LEP  FEL    P+  ++ N ++FAPIGL NM N
Sbjct: 744 EEFALFLSQSKKLL-LSGLNDDLELTLEPFKFELITVSPVVTIEGNSVRFAPIGLVNMLN 783

Query: 790 SSGTIQHLKYNKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDYDGKLSFDLCWT 849
           +SG I+ L YN   VE+ V G G F  Y+S  P  CL +G  VEF ++ D  +   + W+
Sbjct: 804 TSGAIRSLVYNDESVEVGVFGAGEFRVYASKKPVSCLIDGEVVEFGYE-DSMVMVQVPWS 783

BLAST of Cp4.1LG12g06330 vs. TAIR 10
Match: AT5G20250.1 (Raffinose synthase family protein )

HSP 1 Score: 464.9 bits (1195), Expect = 1.4e-130
Identity = 284/846 (33.57%), Postives = 417/846 (49.29%), Query Frame = 0

Query: 8   VHLSNGNISVQGVPLLSDVPSNVFFSPFSSVRSSDAPLPLFQRVHALSHKGGFLG--FDQ 67
           V +S+GN+ ++   +L+ VP NV     ++  S   P+           +G F+G  F++
Sbjct: 7   VRISDGNLIIKNRTILTGVPDNV----ITTSASEAGPV-----------EGVFVGAVFNK 66

Query: 68  TQSQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPE---- 127
            +S    + I  +G      F+S FRFK WW    +G  G DI  ETQ++++   +    
Sbjct: 67  EES----KHIVPIGTLRNSRFMSCFRFKLWWMAQRMGEMGRDIPYETQFLLVESNDGSHL 126

Query: 128 -----------IRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYI 187
                       + Y V +P+IEGSFRS L    +  V +  ESG    K SSF    YI
Sbjct: 127 ESDGANGVECNQKVYTVFLPLIEGSFRSCLQGNVNDEVELCLESGDVDTKRSSFTHSLYI 186

Query: 188 HVCDNPYNLMKEAYATIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGV 247
           H   +P+  + +A  T+++HLN+FR   EK +P +VD FGWCTW+AF+  V   GV  G+
Sbjct: 187 HAGTDPFQTITDAIRTVKLHLNSFRQRHEKKLPGIVDYFGWCTWDAFYQEVTQEGVEAGL 246

Query: 248 NDFFEGGVSLRFLIIDDGWQSINIDGEDPHRDMKYIVLGANQMTARLYKFEECDKFEKYK 307
                GG   +F+IIDDGWQS+  D      D K           RL   +E +KF+K  
Sbjct: 247 KSLAAGGTPPKFVIIDDGWQSVERDATVEAGDEK-----KESPIFRLTGIKENEKFKK-- 306

Query: 308 GGTMLGPNAPPFDPKKPKLLISKSIEIDNAEKNRDNAIQSGVNDVFKFETEVKKLKQELI 367
                                                                       
Sbjct: 307 ------------------------------------------------------------ 366

Query: 368 DIFWNDRDEENNVESNGNGNCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALTGAWGGV 427
                 +D+ N                +G+K   +  + K  GL  +YVWHA+TG WGGV
Sbjct: 367 ------KDDPN----------------VGIKNIVKIAKEK-HGLKYVYVWHAITGYWGGV 426

Query: 428 RPGSTHLKSKITPLKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLFDSMHSYLSEAG 487
           RPG  +      P  S   ++   T    V  ++G +GLV P + Y  ++ +HSYL++AG
Sbjct: 427 RPGEEYGSVMKYPNMSKGVVENDPTWKTDVMTLQG-LGLVSPKKVYKFYNELHSYLADAG 486

Query: 488 ITGVKVDVIHTLEYVSEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLLSSMQQCNDFFFLG 547
           + GVKVDV   LE +    GGRV+L + +++ L  S+ KNF     ++ M    D  +  
Sbjct: 487 VDGVKVDVQCVLETLGGGLGGRVELTRQFHQALDSSVAKNFPDNGCIACMSHNTDALYC- 546

Query: 548 TKQNSIGRASDDFWFHDPHGDPMGMYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVC 607
           +KQ ++ RASDDF+  DP            +H+   AYNS+++G+ +QPDWDMF S H  
Sbjct: 547 SKQAAVIRASDDFYPRDPVS--------HTIHIASVAYNSVFLGEFMQPDWDMFHSVHPA 606

Query: 608 AKFHAGSRAICGGPIYVSDSVGSHDFYLIKKLAYPDGTIPRCRHFALPTRDCLFKNPLFD 667
           A++HA +RAI GGP+YVSDS G H+F L++KL  PDG+I R R    PTRDCLF +P  D
Sbjct: 607 AEYHASARAISGGPLYVSDSPGKHNFELLRKLVLPDGSILRARLPGRPTRDCLFADPARD 666

Query: 668 NKTVLKIWNLNMYGGVIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPE 727
             ++LKIWN+N Y GV+G +NCQGA W   ER+   H      ++ ++   DV    +  
Sbjct: 667 GVSLLKIWNMNKYTGVLGVYNCQGAAWSSTERKNIFHQTKTDSLTGSIRGRDVHSISEAS 726

Query: 728 AAPMGNFVEYVVYLNQAEQILHTTPKSEPLELNLEPSTFELFNFIPLKKLDSNIKFAPIG 787
             P     +  VY +Q+   L   P +  L ++L+    E+F   P+  L   + FAPIG
Sbjct: 727 TDPTTWNGDCAVY-SQSRGELIVMPYNVSLPVSLKIREHEIFTVSPISHLVDGVSFAPIG 732

Query: 788 LTNMFNSSGTIQHLKY--NKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWD-YDG 834
           L NM+NS G I+ L+Y   K  V ++VKG G F +YSS  PK+C+    E+ FE+D   G
Sbjct: 787 LVNMYNSGGAIEGLRYEAEKMKVVMEVKGCGKFGSYSSVKPKRCVVESNEIAFEYDSSSG 732

BLAST of Cp4.1LG12g06330 vs. TAIR 10
Match: AT5G20250.2 (Raffinose synthase family protein )

HSP 1 Score: 464.9 bits (1195), Expect = 1.4e-130
Identity = 284/846 (33.57%), Postives = 417/846 (49.29%), Query Frame = 0

Query: 8   VHLSNGNISVQGVPLLSDVPSNVFFSPFSSVRSSDAPLPLFQRVHALSHKGGFLG--FDQ 67
           V +S+GN+ ++   +L+ VP NV     ++  S   P+           +G F+G  F++
Sbjct: 7   VRISDGNLIIKNRTILTGVPDNV----ITTSASEAGPV-----------EGVFVGAVFNK 66

Query: 68  TQSQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPE---- 127
            +S    + I  +G      F+S FRFK WW    +G  G DI  ETQ++++   +    
Sbjct: 67  EES----KHIVPIGTLRNSRFMSCFRFKLWWMAQRMGEMGRDIPYETQFLLVESNDGSHL 126

Query: 128 -----------IRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYI 187
                       + Y V +P+IEGSFRS L    +  V +  ESG    K SSF    YI
Sbjct: 127 ESDGANGVECNQKVYTVFLPLIEGSFRSCLQGNVNDEVELCLESGDVDTKRSSFTHSLYI 186

Query: 188 HVCDNPYNLMKEAYATIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGV 247
           H   +P+  + +A  T+++HLN+FR   EK +P +VD FGWCTW+AF+  V   GV  G+
Sbjct: 187 HAGTDPFQTITDAIRTVKLHLNSFRQRHEKKLPGIVDYFGWCTWDAFYQEVTQEGVEAGL 246

Query: 248 NDFFEGGVSLRFLIIDDGWQSINIDGEDPHRDMKYIVLGANQMTARLYKFEECDKFEKYK 307
                GG   +F+IIDDGWQS+  D      D K           RL   +E +KF+K  
Sbjct: 247 KSLAAGGTPPKFVIIDDGWQSVERDATVEAGDEK-----KESPIFRLTGIKENEKFKK-- 306

Query: 308 GGTMLGPNAPPFDPKKPKLLISKSIEIDNAEKNRDNAIQSGVNDVFKFETEVKKLKQELI 367
                                                                       
Sbjct: 307 ------------------------------------------------------------ 366

Query: 368 DIFWNDRDEENNVESNGNGNCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALTGAWGGV 427
                 +D+ N                +G+K   +  + K  GL  +YVWHA+TG WGGV
Sbjct: 367 ------KDDPN----------------VGIKNIVKIAKEK-HGLKYVYVWHAITGYWGGV 426

Query: 428 RPGSTHLKSKITPLKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLFDSMHSYLSEAG 487
           RPG  +      P  S   ++   T    V  ++G +GLV P + Y  ++ +HSYL++AG
Sbjct: 427 RPGEEYGSVMKYPNMSKGVVENDPTWKTDVMTLQG-LGLVSPKKVYKFYNELHSYLADAG 486

Query: 488 ITGVKVDVIHTLEYVSEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLLSSMQQCNDFFFLG 547
           + GVKVDV   LE +    GGRV+L + +++ L  S+ KNF     ++ M    D  +  
Sbjct: 487 VDGVKVDVQCVLETLGGGLGGRVELTRQFHQALDSSVAKNFPDNGCIACMSHNTDALYC- 546

Query: 548 TKQNSIGRASDDFWFHDPHGDPMGMYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVC 607
           +KQ ++ RASDDF+  DP            +H+   AYNS+++G+ +QPDWDMF S H  
Sbjct: 547 SKQAAVIRASDDFYPRDPVS--------HTIHIASVAYNSVFLGEFMQPDWDMFHSVHPA 606

Query: 608 AKFHAGSRAICGGPIYVSDSVGSHDFYLIKKLAYPDGTIPRCRHFALPTRDCLFKNPLFD 667
           A++HA +RAI GGP+YVSDS G H+F L++KL  PDG+I R R    PTRDCLF +P  D
Sbjct: 607 AEYHASARAISGGPLYVSDSPGKHNFELLRKLVLPDGSILRARLPGRPTRDCLFADPARD 666

Query: 668 NKTVLKIWNLNMYGGVIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPE 727
             ++LKIWN+N Y GV+G +NCQGA W   ER+   H      ++ ++   DV    +  
Sbjct: 667 GVSLLKIWNMNKYTGVLGVYNCQGAAWSSTERKNIFHQTKTDSLTGSIRGRDVHSISEAS 726

Query: 728 AAPMGNFVEYVVYLNQAEQILHTTPKSEPLELNLEPSTFELFNFIPLKKLDSNIKFAPIG 787
             P     +  VY +Q+   L   P +  L ++L+    E+F   P+  L   + FAPIG
Sbjct: 727 TDPTTWNGDCAVY-SQSRGELIVMPYNVSLPVSLKIREHEIFTVSPISHLVDGVSFAPIG 732

Query: 788 LTNMFNSSGTIQHLKY--NKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWD-YDG 834
           L NM+NS G I+ L+Y   K  V ++VKG G F +YSS  PK+C+    E+ FE+D   G
Sbjct: 787 LVNMYNSGGAIEGLRYEAEKMKVVMEVKGCGKFGSYSSVKPKRCVVESNEIAFEYDSSSG 732

BLAST of Cp4.1LG12g06330 vs. TAIR 10
Match: AT5G20250.3 (Raffinose synthase family protein )

HSP 1 Score: 464.9 bits (1195), Expect = 1.4e-130
Identity = 284/846 (33.57%), Postives = 417/846 (49.29%), Query Frame = 0

Query: 8   VHLSNGNISVQGVPLLSDVPSNVFFSPFSSVRSSDAPLPLFQRVHALSHKGGFLG--FDQ 67
           V +S+GN+ ++   +L+ VP NV     ++  S   P+           +G F+G  F++
Sbjct: 7   VRISDGNLIIKNRTILTGVPDNV----ITTSASEAGPV-----------EGVFVGAVFNK 66

Query: 68  TQSQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPE---- 127
            +S    + I  +G      F+S FRFK WW    +G  G DI  ETQ++++   +    
Sbjct: 67  EES----KHIVPIGTLRNSRFMSCFRFKLWWMAQRMGEMGRDIPYETQFLLVESNDGSHL 126

Query: 128 -----------IRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYI 187
                       + Y V +P+IEGSFRS L    +  V +  ESG    K SSF    YI
Sbjct: 127 ESDGANGVECNQKVYTVFLPLIEGSFRSCLQGNVNDEVELCLESGDVDTKRSSFTHSLYI 186

Query: 188 HVCDNPYNLMKEAYATIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGV 247
           H   +P+  + +A  T+++HLN+FR   EK +P +VD FGWCTW+AF+  V   GV  G+
Sbjct: 187 HAGTDPFQTITDAIRTVKLHLNSFRQRHEKKLPGIVDYFGWCTWDAFYQEVTQEGVEAGL 246

Query: 248 NDFFEGGVSLRFLIIDDGWQSINIDGEDPHRDMKYIVLGANQMTARLYKFEECDKFEKYK 307
                GG   +F+IIDDGWQS+  D      D K           RL   +E +KF+K  
Sbjct: 247 KSLAAGGTPPKFVIIDDGWQSVERDATVEAGDEK-----KESPIFRLTGIKENEKFKK-- 306

Query: 308 GGTMLGPNAPPFDPKKPKLLISKSIEIDNAEKNRDNAIQSGVNDVFKFETEVKKLKQELI 367
                                                                       
Sbjct: 307 ------------------------------------------------------------ 366

Query: 368 DIFWNDRDEENNVESNGNGNCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALTGAWGGV 427
                 +D+ N                +G+K   +  + K  GL  +YVWHA+TG WGGV
Sbjct: 367 ------KDDPN----------------VGIKNIVKIAKEK-HGLKYVYVWHAITGYWGGV 426

Query: 428 RPGSTHLKSKITPLKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLFDSMHSYLSEAG 487
           RPG  +      P  S   ++   T    V  ++G +GLV P + Y  ++ +HSYL++AG
Sbjct: 427 RPGEEYGSVMKYPNMSKGVVENDPTWKTDVMTLQG-LGLVSPKKVYKFYNELHSYLADAG 486

Query: 488 ITGVKVDVIHTLEYVSEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLLSSMQQCNDFFFLG 547
           + GVKVDV   LE +    GGRV+L + +++ L  S+ KNF     ++ M    D  +  
Sbjct: 487 VDGVKVDVQCVLETLGGGLGGRVELTRQFHQALDSSVAKNFPDNGCIACMSHNTDALYC- 546

Query: 548 TKQNSIGRASDDFWFHDPHGDPMGMYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVC 607
           +KQ ++ RASDDF+  DP            +H+   AYNS+++G+ +QPDWDMF S H  
Sbjct: 547 SKQAAVIRASDDFYPRDPVS--------HTIHIASVAYNSVFLGEFMQPDWDMFHSVHPA 606

Query: 608 AKFHAGSRAICGGPIYVSDSVGSHDFYLIKKLAYPDGTIPRCRHFALPTRDCLFKNPLFD 667
           A++HA +RAI GGP+YVSDS G H+F L++KL  PDG+I R R    PTRDCLF +P  D
Sbjct: 607 AEYHASARAISGGPLYVSDSPGKHNFELLRKLVLPDGSILRARLPGRPTRDCLFADPARD 666

Query: 668 NKTVLKIWNLNMYGGVIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPE 727
             ++LKIWN+N Y GV+G +NCQGA W   ER+   H      ++ ++   DV    +  
Sbjct: 667 GVSLLKIWNMNKYTGVLGVYNCQGAAWSSTERKNIFHQTKTDSLTGSIRGRDVHSISEAS 726

Query: 728 AAPMGNFVEYVVYLNQAEQILHTTPKSEPLELNLEPSTFELFNFIPLKKLDSNIKFAPIG 787
             P     +  VY +Q+   L   P +  L ++L+    E+F   P+  L   + FAPIG
Sbjct: 727 TDPTTWNGDCAVY-SQSRGELIVMPYNVSLPVSLKIREHEIFTVSPISHLVDGVSFAPIG 732

Query: 788 LTNMFNSSGTIQHLKY--NKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWD-YDG 834
           L NM+NS G I+ L+Y   K  V ++VKG G F +YSS  PK+C+    E+ FE+D   G
Sbjct: 787 LVNMYNSGGAIEGLRYEAEKMKVVMEVKGCGKFGSYSSVKPKRCVVESNEIAFEYDSSSG 732