Cp4.1LG12g05860 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG12g05860
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Pectinesterase
Location	Cp4.1LG12: 7922749 .. 7925587 (+)
RNA-Seq Expression	Cp4.1LG12g05860
Synteny	Cp4.1LG12g05860

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATATTATAAATCTACTCTTCATCTTCCTTCTTGACAGCCACATAAATCTCCCCTTCATCTTCTTCCCTCCTCTCTCTGTTTCAGACATGTACGGCCTCAGAAACAGAGCTCAAAGCTCTTCAAATCCCACCGCCTCCATTTCCGCCATTTTCACCTTCTTTTCCGTTCTTATCCTTTGCTCCTTCAAACCCATTTCTTCAAATCCCCAAACCCACCAAACCGCTCGATCCCTTTGCAATGGCACTCTGTTTCCAAATCTCTGTTTCTCAAAGCTCTCCTCTTTTCCCCAACTCGCCTCCCTCTCGCCGGAAAAGCTCGCCGGCTCCGCCCTCAACTTCACCAACGACGAGGTTCACGGTGCCTACGCCAATTGCTCCAAGCTCAAGACTCTCTTCTACGGAGGTCTGAGTCCCACTGACCGCCGCGCCCTCGATGACTGCCTTGAGCTTCTCGATGGCAGCATTGCCGAACTCCAAACTTCGATTTACGATCTATCACCGTCGCAGTCCGCCGCCCGCCACTCCCACGATCTGTTGACGTTGGTCAGCGCCGCCATGACTAACCACCGGACTTGTCTTGATGGGTTCTATAATAGTTCCGGGACTGTGAGGAAACGGGTGGAGCTGTATTTGGGGAAAATCGAACAGCATTTGAGTATTGATTTGGCTATGCTGAAGAAAATCCCTGGAGTTAAACGGGCTGCCACGGCGGAAGAGGTGTTGCCGGAGTATGGGGCGGTGAGAGGTGGGTTTCCGATGTGGGTTTCCGTTAAGGATCGGAGATTGTTGCAGGCGGCGGTGAATGAAACAAAGTTCAATTTGGTGGTGGCGAAGGATGGCTCCGGGAATTTCACCACTGTCGGCGAGGCTGTCGCCGCCGCTCCAAATTCGAGCACGACCAGGTTTGTAATATACATAAAAGCAGGGGCCTATTTTGAGAATGTGGAAATAGAACGGAAAAAGACAAACTTGGTGTTCGTCGGCGACGGGATCGGCAAGACGCTGATAAAGGCCAACCGGAATGTCGTTGACGGCTGGACTACCTTCCGATCAGCCACCGTCGGTTAGTTTCCGTTTCACTCTTTTTTTTTTTAATAAATTAATAATTAATTACTTTCATTAAAATGATGAGACCACCTGACGATCATCAGATCTTGCCTCTGAAATAATTTATTTATTTATTAATCATAATTTTGGATCTGTTCTAATATTTTAAAATTAGAAAACGTTTCTTAATTTAATGTGTTTTTTTTTTTAATTTTAGAAAATAAATAGGTATAGAATTGAAACTTAAAAAAGAAAATAAAAATTTAAAATAGAAAAGGGAAATCAGTAGATAATGGTTGAATATAAAATAGACTAAACTAATAAATAAATAAAAAAAATTAAATTTTAAAAATTTAATAATATTTTAAAATTTAAAAAAAAAAATTAAAAATTTAAAAACACTCTTGTTTTTTTGTGGCTGAAATTGTTAATATTTTATTTAAAAAATATCTTTTAATTTTGAGAAATTCCTGTCAAACTTTCAAAATTTCATTAATATAATTTTTAAACTTTTTTAAATGTCATTAAATATTTTAAAAATAATAATTGTAGTATCCATTTAAAAATATTAATAAACTTTTAAAAGTAACTCTAGATAATAGATGGTTTTGATTTATATATTGACGATAAATTTTTTTGAATTTTTTTATTTATAAAAATTAAGGGTTAAGGTTATAGGTGTTTTCAAATAAAAATAAAAATTAAAAGGTATTAATAAAAAAAAATTAATTCAAAAATATTTTTGAAATATTATTGATAGTTTGATAATTTTTAAAACATTTAAAATTATTATTGAATTTTTTGAAAATTTAAGGATGTTTTTTATTAATTATTTTGAAAGTGGCAGAAGGGTAGGGGAAGGCGAGTACAGTGGGCCCAGTGGGCCCAGTATGGCCCAAAAGCCGCTAATATGGGCGAACCGCATGTGGGTTGGATAAGATTGCCAATCTCCGGAAAAAAGGGCAGTTGCCTTTTTCAATCAATTTTTAAATTTATTCTATAAATTTATAAATGTTTTTTAAATCTACTTTTTTATTTGGTCTTATAATTTTTTATTTTTTTTTCGAGAATAATAACATATGTTAGTTACTATGTTCAGCCGCGGTCGGCAATGGGTTCATAGCGAAAGGCATAACATTCGAAAACTTTGCTGGTCCAAGCAAGCACCAAGCCGTAGCCTTCCGAAGCAACTCCGACTTCTCAGCATTTTACCAATGCAGCTTCATCGGCTATCAAGACACTCTCTACGTCCATTCTCTCCGTCAATTCTACCGCGAATGCGACGTCTATGGCACCGTCGACTTCATCTTCGGTAACGCTGCAGTCGTCTTCCAAAACTGCAATCTCTACGCTCGAAAACCCAACTCAAACCAACGCAACATATTCACAGCTCAAGGTCGTGAGGATCCTAACCAAAATACCGGAATCTCAATCCTCAATTGCAAGGTTGAAGCCGCCTCTGATTTGATCCCAGTTCTTCCCTCTTTCAGAACTTACTTAGGTCGACCCTGGAAGCTCTACTCGAGGACGGTTTTTCTCAAGTCATTTATTGGGCAGTTGGTTGAGCCAGTCGGATGGCTTGAGTGGAGTGGGACATTCGCATTAGATACTTTGTATTATGGGGAGTATTTGAATCGTGGGCCTGGTTCTAATACAACTGGACGAGTCACTTGGCCAGGTTATCGGGTCATTACTAACTCAACGGAAGCGAGTCAGTTTACGGTGGAGGCGTTTATACAAGGTAGTGAGTGGCTGAACTCGACCGAAATTCCTTTCTTCTCCGGCTTGGCT

mRNA sequence

AAATATTATAAATCTACTCTTCATCTTCCTTCTTGACAGCCACATAAATCTCCCCTTCATCTTCTTCCCTCCTCTCTCTGTTTCAGACATGTACGGCCTCAGAAACAGAGCTCAAAGCTCTTCAAATCCCACCGCCTCCATTTCCGCCATTTTCACCTTCTTTTCCGTTCTTATCCTTTGCTCCTTCAAACCCATTTCTTCAAATCCCCAAACCCACCAAACCGCTCGATCCCTTTGCAATGGCACTCTGTTTCCAAATCTCTGTTTCTCAAAGCTCTCCTCTTTTCCCCAACTCGCCTCCCTCTCGCCGGAAAAGCTCGCCGGCTCCGCCCTCAACTTCACCAACGACGAGGTTCACGGTGCCTACGCCAATTGCTCCAAGCTCAAGACTCTCTTCTACGGAGGTCTGAGTCCCACTGACCGCCGCGCCCTCGATGACTGCCTTGAGCTTCTCGATGGCAGCATTGCCGAACTCCAAACTTCGATTTACGATCTATCACCGTCGCAGTCCGCCGCCCGCCACTCCCACGATCTGTTGACGTTGGTCAGCGCCGCCATGACTAACCACCGGACTTGTCTTGATGGGTTCTATAATAGTTCCGGGACTGTGAGGAAACGGGTGGAGCTGTATTTGGGGAAAATCGAACAGCATTTGAGTATTGATTTGGCTATGCTGAAGAAAATCCCTGGAGTTAAACGGGCTGCCACGGCGGAAGAGGTGTTGCCGGAGTATGGGGCGGTGAGAGGTGGGTTTCCGATGTGGGTTTCCGTTAAGGATCGGAGATTGTTGCAGGCGGCGGTGAATGAAACAAAGTTCAATTTGGTGGTGGCGAAGGATGGCTCCGGGAATTTCACCACTGTCGGCGAGGCTGTCGCCGCCGCTCCAAATTCGAGCACGACCAGGTTTGTAATATACATAAAAGCAGGGGCCTATTTTGAGAATGTGGAAATAGAACGGAAAAAGACAAACTTGGTGTTCGTCGGCGACGGGATCGGCAAGACGCTGATAAAGGCCAACCGGAATGTCGTTGACGGCTGGACTACCTTCCGATCAGCCACCGTCGGTAGTGAGTGGCTGAACTCGACCGAAATTCCTTTCTTCTCCGGCTTGGCT

Coding sequence (CDS)

ATGTACGGCCTCAGAAACAGAGCTCAAAGCTCTTCAAATCCCACCGCCTCCATTTCCGCCATTTTCACCTTCTTTTCCGTTCTTATCCTTTGCTCCTTCAAACCCATTTCTTCAAATCCCCAAACCCACCAAACCGCTCGATCCCTTTGCAATGGCACTCTGTTTCCAAATCTCTGTTTCTCAAAGCTCTCCTCTTTTCCCCAACTCGCCTCCCTCTCGCCGGAAAAGCTCGCCGGCTCCGCCCTCAACTTCACCAACGACGAGGTTCACGGTGCCTACGCCAATTGCTCCAAGCTCAAGACTCTCTTCTACGGAGGTCTGAGTCCCACTGACCGCCGCGCCCTCGATGACTGCCTTGAGCTTCTCGATGGCAGCATTGCCGAACTCCAAACTTCGATTTACGATCTATCACCGTCGCAGTCCGCCGCCCGCCACTCCCACGATCTGTTGACGTTGGTCAGCGCCGCCATGACTAACCACCGGACTTGTCTTGATGGGTTCTATAATAGTTCCGGGACTGTGAGGAAACGGGTGGAGCTGTATTTGGGGAAAATCGAACAGCATTTGAGTATTGATTTGGCTATGCTGAAGAAAATCCCTGGAGTTAAACGGGCTGCCACGGCGGAAGAGGTGTTGCCGGAGTATGGGGCGGTGAGAGGTGGGTTTCCGATGTGGGTTTCCGTTAAGGATCGGAGATTGTTGCAGGCGGCGGTGAATGAAACAAAGTTCAATTTGGTGGTGGCGAAGGATGGCTCCGGGAATTTCACCACTGTCGGCGAGGCTGTCGCCGCCGCTCCAAATTCGAGCACGACCAGGTTTGTAATATACATAAAAGCAGGGGCCTATTTTGAGAATGTGGAAATAGAACGGAAAAAGACAAACTTGGTGTTCGTCGGCGACGGGATCGGCAAGACGCTGATAAAGGCCAACCGGAATGTCGTTGACGGCTGGACTACCTTCCGATCAGCCACCGTCGGTAGTGAGTGGCTGAACTCGACCGAAATTCCTTTCTTCTCCGGCTTGGCT

Protein sequence

MYGLRNRAQSSSNPTASISAIFTFFSVLILCSFKPISSNPQTHQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLELLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIGKTLIKANRNVVDGWTTFRSATVGSEWLNSTEIPFFSGLA

Homology

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BLAST of Cp4.1LG12g05860 vs. ExPASy Swiss-Prot
Match: O81301 (Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3702 GN=PME40 PE=2 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 6.0e-58
Identity = 131/263 (49.81%), Postives = 168/263 (63.88%), Query Frame = 0


Query: 76  KLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLELLDGSIAELQTSIYD 135
           +L  +ALN T   V+ + +N S L       LS  D  A DDCLELLD ++ +L T+I  
Sbjct: 39  ELVVTALNQTISNVNLSSSNFSDLLQRLGSNLSHRDLCAFDDCLELLDDTVFDLTTAI-- 98

Query: 136 LSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGT------------VRKRVELYLG 195
            S  +S +   H++  L+SAAMTN RTCLDGF +S               V + ++  L 
Sbjct: 99  -SKLRSHSPELHNVKMLLSAAMTNTRTCLDGFASSDNDENLNNNDNKTYGVAESLKESLF 158

Query: 196 KIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKF 255
            I  H+S  LAML+ IPG       E+V         GFPMWVS  DR LLQ  V+ETK 
Sbjct: 159 NISSHVSDSLAMLENIPGHIPGKVKEDV---------GFPMWVSGSDRNLLQDPVDETKV 218

Query: 256 NLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIG 315
           NLVVA++G+GN+TT+GEA++AAPNSS TRFVIYIK G YFEN+EI R+KT ++F+GDGIG
Sbjct: 219 NLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGDGIG 278

Query: 316 KTLIKANRNVVDGWTTFRSATVG 327
           +T+IKANR+  DGWT F SATVG
Sbjct: 279 RTVIKANRSYADGWTAFHSATVG 289

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BLAST of Cp4.1LG12g05860 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 201.8 bits (512), Expect = 1.2e-50
Identity = 129/305 (42.30%), Postives = 175/305 (57.38%), Query Frame = 0


Query: 42  THQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKT 101
           +H   +S+C+ TL+P LCFS +++       S +++  ++LN T   V   Y    KL  
Sbjct: 58  SHAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKL-I 117

Query: 102 LFYGGLSPTDRRALDDCLELLDGSIAELQTSIYDLS--PSQSAAR-HSHDLLTLVSAAMT 161
               GL+P +  AL DCLE +D ++ EL  ++ DL   P Q + R H+ DL TL+S+A+T
Sbjct: 118 AKRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAIT 177

Query: 162 NHRTCLDGFYNSSGTVRKRVELYLGK--IEQHLSIDLAMLKKIPGV---------KRAAT 221
           N  TCLDGF       + R  L  G+  +E   S  LAM+K +            K +  
Sbjct: 178 NQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTF 237

Query: 222 AEEVLPEYGAVRG-----GFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAV 281
                 +   V G     G+P W+SV DRRLLQ +    K +  VA DGSG+FTTV  AV
Sbjct: 238 TNNNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQGST--IKADATVADDGSGDFTTVAAAV 297

Query: 282 AAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIGKTLIKANRNVVDGWTTFRS 328
           AAAP  S  RFVI+IKAG Y ENVE+ +KKTN++F+GDG GKT+I  +RNVVDG TTF S
Sbjct: 298 AAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHS 357

[Close]

BLAST of Cp4.1LG12g05860 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 2.7e-50
Identity = 114/253 (45.06%), Postives = 163/253 (64.43%), Query Frame = 0


Query: 82  LNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLELLDGSIAELQTSIYDLSPSQS 141
           LN T  +V+ A +N S L+T  +  L+  +R A +DCL LLD +I++L+T++ DL   +S
Sbjct: 61  LNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLDDTISDLETAVSDL---RS 120

Query: 142 AARHSHDLLTLVSAAMTNHRTCLDGFYNSSG--------TVRKRVELYLGKIEQHLSIDL 201
           ++   +D+  L++  MT   TCLDGF  S           + + ++  +  I  +LS  L
Sbjct: 121 SSLEFNDISMLLTNVMTYQDTCLDGFSTSDNENNNDMTYELPENLKEIILDISNNLSNSL 180

Query: 202 AMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSG 261
            ML+ I   K +  + EV  EY       P W+S  D+RLL+A V ET +NL VA DG+G
Sbjct: 181 HMLQVISRKKPSPKSSEVDVEY-------PSWLSENDQRLLEAPVQETNYNLSVAIDGTG 240

Query: 262 NFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIGKTLIKANRNV 321
           NFTT+ +AV AAPN S TRF+IYIK G YFENVE+ +KKT ++F+GDGIGKT+IKANR+ 
Sbjct: 241 NFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVIKANRSR 300

Query: 322 VDGWTTFRSATVG 327
           +DGW+TF++ TVG
Sbjct: 301 IDGWSTFQTPTVG 303

[Close]

BLAST of Cp4.1LG12g05860 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 1.0e-49
Identity = 122/300 (40.67%), Postives = 176/300 (58.67%), Query Frame = 0


Query: 37  SSNPQTHQTARSLCNGTLFPNLCFSKLSSFPQLAS--LSPEKLAGSALNFTNDEVHGAYA 96
           + N   H   +S C+ T +P+LCFS +++ P+ +    S + +   +LN T   V   Y 
Sbjct: 60  NGNEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYF 119

Query: 97  NCSKLKTLFYGGLSPTDRRALDDCLELLDGSIAELQTSIYDLSP---SQSAARHSHDLLT 156
              KL  L    L+  ++ AL DCLE +D ++ EL  ++ DL      +S ++H+ DL T
Sbjct: 120 GIQKL--LKRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKT 179

Query: 157 LVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGK--IEQHLSIDLAMLKKIPG----VKRA 216
           L+SAAMTN  TCLDGF +       R  L  G+  +E+  S  LAM+K +      + R 
Sbjct: 180 LMSAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRT 239

Query: 217 ATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAA 276
           +   +++ E   V  G+P W+S  DRRLLQ+  +    N+VVA DGSGNF TV  +VAAA
Sbjct: 240 SNNRKLIEETSTV-DGWPAWLSTGDRRLLQS--SSVTPNVVVAADGSGNFKTVAASVAAA 299

Query: 277 PNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIGKTLIKANRNVVDGWTTFRSATV 326
           P   T R++I IKAG Y ENVE+ +K  N++F+GDG  +T+I  +RNVVDG TTF+SATV
Sbjct: 300 PQGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATV 354

[Close]

BLAST of Cp4.1LG12g05860 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 8.7e-49
Identity = 122/299 (40.80%), Postives = 173/299 (57.86%), Query Frame = 0


Query: 37  SSNPQTHQTARSLCNGTLFPNLCFSKLSSFPQLAS--LSPEKLAGSALNFTNDEVHGAYA 96
           + N   H   +S C+ T +P+LCFS +++ P+ +    S + +   +LN T   V   Y 
Sbjct: 60  NGNEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYF 119

Query: 97  NCSKLKTLFYGGLSPTDRRALDDCLELLDGSIAELQTSIYDLSP---SQSAARHSHDLLT 156
              KL  L    L+  ++ AL DCLE +D ++ EL  ++ DL      +S ++H+ DL T
Sbjct: 120 GIQKL--LKRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKT 179

Query: 157 LVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGK--IEQHLSIDLAMLKKIPG----VKRA 216
           L+SAAMTN  TCLDGF +       R  L  G+  +E+  S  LAM+K +      + R 
Sbjct: 180 LMSAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRT 239

Query: 217 ATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAA 276
           +   ++  E   V  G+P W+S  DRRLLQ+  +    N VVA DGSGNF TV  AVAAA
Sbjct: 240 SNNRKLTEETSTV-DGWPAWLSPGDRRLLQS--SSVTPNAVVAADGSGNFKTVAAAVAAA 299

Query: 277 PNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIGKTLIKANRNVVDGWTTFRSAT 325
           P   T R++I IKAG Y ENVE+ +K  N++F+GDG  +T+I  +RNVVDG TTF+SAT
Sbjct: 300 PQGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSAT 353

[Close]

BLAST of Cp4.1LG12g05860 vs. NCBI nr
Match: XP_023548978.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 644 bits (1662), Expect = 4.10e-229
Identity = 325/327 (99.39%), Postives = 326/327 (99.69%), Query Frame = 0


Query: 1   MYGLRNRAQSSSNPTASISAIFTFFSVLILCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLRNRAQSSSNPTASISAIFTFFSVLILCSFKPISSNPQTHQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRNRAQSSSNPTASISAIFTFFSVLILCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLE 120

Query: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180
           LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGD 300
           TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGD 300

Query: 301 GIGKTLIKANRNVVDGWTTFRSATVGS 327
           GIGKTLIKANRNVVDGWTTFRSATV +
Sbjct: 301 GIGKTLIKANRNVVDGWTTFRSATVAA 327

[Close]

BLAST of Cp4.1LG12g05860 vs. NCBI nr
Match: KAG6575805.1 (putative pectinesterase/pectinesterase inhibitor 40, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 634 bits (1635), Expect = 5.23e-225
Identity = 318/327 (97.25%), Postives = 325/327 (99.39%), Query Frame = 0


Query: 1   MYGLRNRAQSSSNPTASISAIFTFFSVLILCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLR+RAQSSSNPTASISAIFTFFSV+ LCSFKPISSNPQTHQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTFFSVIFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFTN+EVHGAYANCSKLKTLFYGGL+PTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNNEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180
           LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGD 300
           TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNL+FVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKANRNVVDGWTTFRSATVGS 327
           GIGKTLIKA+RNVVDGWTTFRSATV +
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATVAA 327

[Close]

BLAST of Cp4.1LG12g05860 vs. NCBI nr
Match: XP_022953706.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 633 bits (1632), Expect = 1.49e-224
Identity = 318/325 (97.85%), Postives = 323/325 (99.38%), Query Frame = 0


Query: 1   MYGLRNRAQSSSNPTASISAIFTFFSVLILCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLR+RAQSSSNPTASISAIFTFFSVL LCSFKPISSNPQTHQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGL+PTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180
           LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQA+VNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQASVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGD 300
           TKFNLVVAKDGSGNFTTVG AVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNL+FVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGAAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKANRNVVDGWTTFRSATV 325
           GIGKTLIKA+RNVVDGWTTFRSATV
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATV 325

[Close]

BLAST of Cp4.1LG12g05860 vs. NCBI nr
Match: KAG7014347.1 (putative pectinesterase/pectinesterase inhibitor 40 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 632 bits (1630), Expect = 3.01e-224
Identity = 318/327 (97.25%), Postives = 323/327 (98.78%), Query Frame = 0


Query: 1   MYGLRNRAQSSSNPTASISAIFTFFSVLILCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLR+RAQSSSNPTASISAIFTFFSVL LCSFKPISSNPQTHQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGL+PTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180
           LLD SIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSG VRKRVEL
Sbjct: 121 LLDDSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGNVRKRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGD 300
           TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNL+FVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKANRNVVDGWTTFRSATVGS 327
           GIGKTLIKA+RNVVDGWTTFRSATV +
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATVAA 327

[Close]

BLAST of Cp4.1LG12g05860 vs. NCBI nr
Match: XP_022991224.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 630 bits (1625), Expect = 1.73e-223
Identity = 318/327 (97.25%), Postives = 323/327 (98.78%), Query Frame = 0


Query: 1   MYGLRNRAQSSSNPTASISAIFTFFSVLILCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLR+RAQSSSNPTASISAIFT FSVL L SFKPISSNPQTHQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTLFSVLFLFSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGL+PTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180
           LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGD 300
           TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNL+FVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKANRNVVDGWTTFRSATVGS 327
           GIGKTLIKA+RNVVDGWTTFRSATV +
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATVAA 327

[Close]

BLAST of Cp4.1LG12g05860 vs. ExPASy TrEMBL
Match: A0A6J1GNU4 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456156 PE=3 SV=1)

HSP 1 Score: 633 bits (1632), Expect = 7.23e-225
Identity = 318/325 (97.85%), Postives = 323/325 (99.38%), Query Frame = 0


Query: 1   MYGLRNRAQSSSNPTASISAIFTFFSVLILCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLR+RAQSSSNPTASISAIFTFFSVL LCSFKPISSNPQTHQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGL+PTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180
           LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQA+VNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQASVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGD 300
           TKFNLVVAKDGSGNFTTVG AVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNL+FVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGAAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKANRNVVDGWTTFRSATV 325
           GIGKTLIKA+RNVVDGWTTFRSATV
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATV 325

[Close]

BLAST of Cp4.1LG12g05860 vs. ExPASy TrEMBL
Match: A0A6J1JU83 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487942 PE=3 SV=1)

HSP 1 Score: 630 bits (1625), Expect = 8.39e-224
Identity = 318/327 (97.25%), Postives = 323/327 (98.78%), Query Frame = 0


Query: 1   MYGLRNRAQSSSNPTASISAIFTFFSVLILCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLR+RAQSSSNPTASISAIFT FSVL L SFKPISSNPQTHQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTLFSVLFLFSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGL+PTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180
           LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGD 300
           TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNL+FVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKANRNVVDGWTTFRSATVGS 327
           GIGKTLIKA+RNVVDGWTTFRSATV +
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATVAA 327

[Close]

BLAST of Cp4.1LG12g05860 vs. ExPASy TrEMBL
Match: A0A5D3D1W6 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001300 PE=3 SV=1)

HSP 1 Score: 546 bits (1408), Expect = 8.11e-191
Identity = 282/325 (86.77%), Postives = 291/325 (89.54%), Query Frame = 0


Query: 1   MYGLRNRAQSSSNPTASISAIFTFFSVLILCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLRNRAQ SSNPT SIS I TFF +  L S KPISSNPQ HQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRNRAQISSNPT-SISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFT  EV  AYANC+ LKT  Y GL+PTDR ALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLE 120

Query: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180
           LLD SIAEL+ SI DL PSQSAARHSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR RVEL
Sbjct: 121 LLDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKI QHLSIDLAMLKKIPGV RA   EEVLPEYGAV+GGFP WVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIGQHLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGD 300
           TKFNLVVAKDGSGNFTTV EAVAAAPNSSTTRFVIYIKAGAYFENVEI R K+NL+FVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGD 300

Query: 301 GIGKTLIKANRNVVDGWTTFRSATV 325
           GIGKTLIK +RNVVDGWTTFRSATV
Sbjct: 301 GIGKTLIKGDRNVVDGWTTFRSATV 324

[Close]

BLAST of Cp4.1LG12g05860 vs. ExPASy TrEMBL
Match: A0A1S3BSU3 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492792 PE=3 SV=1)

HSP 1 Score: 546 bits (1408), Expect = 8.11e-191
Identity = 282/325 (86.77%), Postives = 291/325 (89.54%), Query Frame = 0


Query: 1   MYGLRNRAQSSSNPTASISAIFTFFSVLILCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLRNRAQ SSNPT SIS I TFF +  L S KPISSNPQ HQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRNRAQISSNPT-SISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFT  EV  AYANC+ LKT  Y GL+PTDR ALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLE 120

Query: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180
           LLD SIAEL+ SI DL PSQSAARHSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR RVEL
Sbjct: 121 LLDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKI QHLSIDLAMLKKIPGV RA   EEVLPEYGAV+GGFP WVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIGQHLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGD 300
           TKFNLVVAKDGSGNFTTV EAVAAAPNSSTTRFVIYIKAGAYFENVEI R K+NL+FVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGD 300

Query: 301 GIGKTLIKANRNVVDGWTTFRSATV 325
           GIGKTLIK +RNVVDGWTTFRSATV
Sbjct: 301 GIGKTLIKGDRNVVDGWTTFRSATV 324

[Close]

BLAST of Cp4.1LG12g05860 vs. ExPASy TrEMBL
Match: A0A0A0K5K8 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405320 PE=3 SV=1)

HSP 1 Score: 486 bits (1250), Expect = 4.80e-166
Identity = 257/329 (78.12%), Postives = 280/329 (85.11%), Query Frame = 0


Query: 8   AQSSSNPTASISAIFTFFSVLILCSFKPISSNP---------QT-HQTARSLCNGTLFPN 67
           + SSS+ ++S S+  +  S     S    SS+P         QT HQTARSLCNGTLFP+
Sbjct: 53  SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSPPPPPPLPLFQTFHQTARSLCNGTLFPD 112

Query: 68  LCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDD 127
           LCFSKLSSFPQLASLSPEKLAGSALNFT+ EV  AY NC+ LKT  Y GL+PTDR ALDD
Sbjct: 113 LCFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDD 172

Query: 128 CLELLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKR 187
           CLELLDGSIAEL+ SI+DL+PSQS A HSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR R
Sbjct: 173 CLELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSR 232

Query: 188 VELYLGKIEQHLSIDLAMLKKIPGVKRAATAE-EVLPEYGAVRGGFPMWVSVKDRRLLQA 247
           VELYLGKI QHLSIDLAMLKKIPGV RA   + E+LPEYGAV+GGFP WVSVKDRRLLQA
Sbjct: 233 VELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEMLPEYGAVKGGFPKWVSVKDRRLLQA 292

Query: 248 AVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLV 307
           AVNETKFN+VVAKDGSGNFTTV EAVAAAPN+STTRFVIYIKAGAYFENVEI R K+NL+
Sbjct: 293 AVNETKFNMVVAKDGSGNFTTVSEAVAAAPNASTTRFVIYIKAGAYFENVEIGRAKSNLM 352

Query: 308 FVGDGIGKTLIKANRNVVDGWTTFRSATV 325
           FVGDGIGKTLIKA+RNVVDGWTTFRSATV
Sbjct: 353 FVGDGIGKTLIKADRNVVDGWTTFRSATV 381

[Close]

BLAST of Cp4.1LG12g05860 vs. TAIR 10
Match: AT4G02320.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 226.1 bits (575), Expect = 4.3e-59
Identity = 131/263 (49.81%), Postives = 168/263 (63.88%), Query Frame = 0


Query: 76  KLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLELLDGSIAELQTSIYD 135
           +L  +ALN T   V+ + +N S L       LS  D  A DDCLELLD ++ +L T+I  
Sbjct: 39  ELVVTALNQTISNVNLSSSNFSDLLQRLGSNLSHRDLCAFDDCLELLDDTVFDLTTAI-- 98

Query: 136 LSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGT------------VRKRVELYLG 195
            S  +S +   H++  L+SAAMTN RTCLDGF +S               V + ++  L 
Sbjct: 99  -SKLRSHSPELHNVKMLLSAAMTNTRTCLDGFASSDNDENLNNNDNKTYGVAESLKESLF 158

Query: 196 KIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKF 255
            I  H+S  LAML+ IPG       E+V         GFPMWVS  DR LLQ  V+ETK 
Sbjct: 159 NISSHVSDSLAMLENIPGHIPGKVKEDV---------GFPMWVSGSDRNLLQDPVDETKV 218

Query: 256 NLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIG 315
           NLVVA++G+GN+TT+GEA++AAPNSS TRFVIYIK G YFEN+EI R+KT ++F+GDGIG
Sbjct: 219 NLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGDGIG 278

Query: 316 KTLIKANRNVVDGWTTFRSATVG 327
           +T+IKANR+  DGWT F SATVG
Sbjct: 279 RTVIKANRSYADGWTAFHSATVG 289

[Close]

BLAST of Cp4.1LG12g05860 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 201.8 bits (512), Expect = 8.7e-52
Identity = 129/305 (42.30%), Postives = 175/305 (57.38%), Query Frame = 0


Query: 42  THQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKT 101
           +H   +S+C+ TL+P LCFS +++       S +++  ++LN T   V   Y    KL  
Sbjct: 58  SHAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKL-I 117

Query: 102 LFYGGLSPTDRRALDDCLELLDGSIAELQTSIYDLS--PSQSAAR-HSHDLLTLVSAAMT 161
               GL+P +  AL DCLE +D ++ EL  ++ DL   P Q + R H+ DL TL+S+A+T
Sbjct: 118 AKRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAIT 177

Query: 162 NHRTCLDGFYNSSGTVRKRVELYLGK--IEQHLSIDLAMLKKIPGV---------KRAAT 221
           N  TCLDGF       + R  L  G+  +E   S  LAM+K +            K +  
Sbjct: 178 NQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTF 237

Query: 222 AEEVLPEYGAVRG-----GFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAV 281
                 +   V G     G+P W+SV DRRLLQ +    K +  VA DGSG+FTTV  AV
Sbjct: 238 TNNNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQGST--IKADATVADDGSGDFTTVAAAV 297

Query: 282 AAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIGKTLIKANRNVVDGWTTFRS 328
           AAAP  S  RFVI+IKAG Y ENVE+ +KKTN++F+GDG GKT+I  +RNVVDG TTF S
Sbjct: 298 AAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHS 357

[Close]

BLAST of Cp4.1LG12g05860 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 200.7 bits (509), Expect = 1.9e-51
Identity = 114/253 (45.06%), Postives = 163/253 (64.43%), Query Frame = 0


Query: 82  LNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLELLDGSIAELQTSIYDLSPSQS 141
           LN T  +V+ A +N S L+T  +  L+  +R A +DCL LLD +I++L+T++ DL   +S
Sbjct: 61  LNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLDDTISDLETAVSDL---RS 120

Query: 142 AARHSHDLLTLVSAAMTNHRTCLDGFYNSSG--------TVRKRVELYLGKIEQHLSIDL 201
           ++   +D+  L++  MT   TCLDGF  S           + + ++  +  I  +LS  L
Sbjct: 121 SSLEFNDISMLLTNVMTYQDTCLDGFSTSDNENNNDMTYELPENLKEIILDISNNLSNSL 180

Query: 202 AMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSG 261
            ML+ I   K +  + EV  EY       P W+S  D+RLL+A V ET +NL VA DG+G
Sbjct: 181 HMLQVISRKKPSPKSSEVDVEY-------PSWLSENDQRLLEAPVQETNYNLSVAIDGTG 240

Query: 262 NFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIGKTLIKANRNV 321
           NFTT+ +AV AAPN S TRF+IYIK G YFENVE+ +KKT ++F+GDGIGKT+IKANR+ 
Sbjct: 241 NFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVIKANRSR 300

Query: 322 VDGWTTFRSATVG 327
           +DGW+TF++ TVG
Sbjct: 301 IDGWSTFQTPTVG 303

[Close]

BLAST of Cp4.1LG12g05860 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 186.4 bits (472), Expect = 3.8e-47
Identity = 120/316 (37.97%), Postives = 170/316 (53.80%), Query Frame = 0


Query: 39  NPQTHQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSK 98
           +P +H   RS C+ T +P LC S + +   +   S + +  +++N T   V   Y    K
Sbjct: 52  SPSSHAVLRSSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKK 111

Query: 99  LKTLFYGGLSPTDRRALDDCLELLDGSIAELQTSIYDLS--PSQSAAR-HSHDLLTLVSA 158
           L      GL+P ++ AL DCLE +D ++ EL  ++ DL   P++   R H+ DL TL+S+
Sbjct: 112 L-IKKRKGLTPREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISS 171

Query: 159 AMTNHRTCLDGFYNSSGTVRKRVELYLGKI--EQHLSIDLAMLKKIPGV----------- 218
           A+TN  TCLDGF +     + R  L  G+I  E   S  LAM+K +              
Sbjct: 172 AITNQETCLDGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKI 231

Query: 219 -----------KRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDG 278
                      +    A ++         G+P W+S  DRRLLQ   +  K +  VA DG
Sbjct: 232 TSNNRKLKEENQETTVAVDIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADG 291

Query: 279 SGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIGKTLIKANR 328
           SG F TV  AVAAAP +S  R+VI+IKAG Y ENVE+ +KK N++F+GDG  +T+I  +R
Sbjct: 292 SGTFKTVAAAVAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSR 351

[Close]

BLAST of Cp4.1LG12g05860 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 161.8 bits (408), Expect = 9.9e-40
Identity = 104/293 (35.49%), Postives = 150/293 (51.19%), Query Frame = 0


Query: 44  QTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLF 103
           Q     C+ +L+PNLC   L  FP   +    +L   + N T  +   A    S   T+ 
Sbjct: 73  QAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSS---TIT 132

Query: 104 YGGLSPTDRRALDDCLELLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTC 163
           Y  + P  R A D CLELLD S+  L  ++  +        HS D++T +S+AMTNH TC
Sbjct: 133 YTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHS-DVMTWLSSAMTNHDTC 192

Query: 164 LDGFYNSSGTVRKRVELYLGKIE-------QHLSIDLAMLKKIPGV----KRAATAEEVL 223
            DGF    G   +  +  +G ++         L+I    +K + GV     R     E  
Sbjct: 193 TDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEET 252

Query: 224 PEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTR 283
            E        P W+  +DR LL    +  + ++ V+KDGSG F T+ EA+  AP  S+ R
Sbjct: 253 EE-------LPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRR 312

Query: 284 FVIYIKAGAY-FENVEIERKKTNLVFVGDGIGKTLIKANRNVVDGWTTFRSAT 325
           FVIY+KAG Y  EN+++ RKKTNL+F+GDG GKT+I   +++ D  TTF +AT
Sbjct: 313 FVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTAT 354

The following BLAST results are available for this feature:

BLAST of Cp4.1LG12g05860 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81301	6.0e-58	49.81	Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3...	[more]
Q42534	1.2e-50	42.30	Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2	[more]
O81415	2.7e-50	45.06	Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3...	[more]
P83948	1.0e-49	40.67	Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1	[more]
O04886	8.7e-49	40.80	Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1	[more]

BLAST of Cp4.1LG12g05860 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023548978.1	4.10e-229	99.39	pectinesterase-like [Cucurbita pepo subsp. pepo]	[more]
KAG6575805.1	5.23e-225	97.25	putative pectinesterase/pectinesterase inhibitor 40, partial [Cucurbita argyrosp...	[more]
XP_022953706.1	1.49e-224	97.85	pectinesterase-like [Cucurbita moschata]	[more]
KAG7014347.1	3.01e-224	97.25	putative pectinesterase/pectinesterase inhibitor 40 [Cucurbita argyrosperma subs...	[more]
XP_022991224.1	1.73e-223	97.25	pectinesterase-like [Cucurbita maxima]	[more]

BLAST of Cp4.1LG12g05860 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GNU4	7.23e-225	97.85	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456156 PE=3 SV=1	[more]
A0A6J1JU83	8.39e-224	97.25	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487942 PE=3 SV=1	[more]
A0A5D3D1W6	8.11e-191	86.77	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00130...	[more]
A0A1S3BSU3	8.11e-191	86.77	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492792 PE=3 SV=1	[more]
A0A0A0K5K8	4.80e-166	78.12	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405320 PE=3 SV=1	[more]

BLAST of Cp4.1LG12g05860 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G02320.1	4.3e-59	49.81	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G53830.1	8.7e-52	42.30	pectin methylesterase 2	[more]
AT4G02300.1	1.9e-51	45.06	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G14310.1	3.8e-47	37.97	pectin methylesterase 3	[more]
AT5G53370.1	9.9e-40	35.49	pectin methylesterase PCR fragment F	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 41..195 e-value: 6.3E-29 score: 112.1
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 47..194 e-value: 3.3E-24 score: 85.9
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 20..198 e-value: 3.7E-25 score: 86.3
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 244..327 e-value: 3.9E-29 score: 101.3
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 238..334 e-value: 4.4E-27 score: 97.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 44..201 e-value: 3.0E-28 score: 100.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 47..200
None	No IPR available	PANTHER	PTHR31707:SF257	PECTINESTERASE	coord: 40..326
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 40..326
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 48..201 e-value: 1.09498E-36 score: 127.94
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 242..326

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG12g05860.1	Cp4.1LG12g05860.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity