Cp4.1LG12g04780 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG12g04780
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionprotein WHAT'S THIS FACTOR 1 homolog
LocationCp4.1LG12: 4613225 .. 4614610 (+)
RNA-Seq ExpressionCp4.1LG12g04780
SyntenyCp4.1LG12g04780
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCGCACATTTCATTCTTCCGCACAGAGCTTACTTTTCTGTTAAATCTGAATTTTGGGGTAAGAATTTGGATTTTCGGAACAGAAATGATAGTTTTAGTGATAATTCAAGAAAATCTCATGTGTATTTTCAACCGATTAGAGCTGGTGTGAAATGGAGGAAGGAGCTTCCGTTTGATAATGTAATACAGAGGGATAAGAAGCTGAAATTGGTTACTCGGATTAGGAAGATTCTAGTTCAGCATCATGATAATGTTATGTCACTTAGGGGTTTGGGTAAATTCAGGAGAGATTTAGGTGTACAGGAAAAGACGAGGCTAATTGAGTTGTTGAAGATGTTCCCTGCAGTATTTGAGATTGTAGAAGAAGAAGCGTTTTCTTTGAAATTTAAATTGACGGAAGAGGCTGAAAGGTTGTACTTCGAGGAGCTGAAGATCAGAAATGAAATGGAAGGTTTGTTGGTTACTAAGCTGAGGAAGTTACTGATGATGTCTGCTGATAAGCGAATATTGTTGGAGAAAATTGCACATTTGAGGACTGATTTTGGTCTTCCTTCCGAGTTTCGTGACACGATTTGCCATCGTTATCCTCAGTACTTTAGAGTTGTGGCAACCGAGAGCGGTCCAGCTCTCGAATTGACACACTGGGATCCTGAACTTGCTGTTTCTGCAGCAGAGTTAGCCGAGGAGGAGAACCGAGCTAGAGAATTGGAAGAGAAGAATTTGATTATTGACCGACCGCTGAAGTTTAATCGAGTGAAGCTACCTAAGGGGCTTAAGCTTTCGAAGAATGAGATGAGGAAAATGAATGAGTTTAGGGACATTCCTTATATCTCTCCGTATTCTGATTTCTCAGGGATTAAGGCAGGTACACCCCAGAAAGAGAAACATGCTTGTGGGGTTGTTCATGAGATTTTAAGCCTTACACTCGAGAAGAGAACTCTTGTTGATCATTTCACTCATTTCCGGGAGGAGTTTAGATTCTCCCAGCAGTTGAGGGGGATGCTAATAAGGCATCCCAATATGTTTTATGTCTCGTTGAAAGGGGATAAAAACTCGGTTTTCCTCAGAGAAGCATATCGCGATTCTCAGTTGATCGACAAAGATCGGTTGTTAATCATAAAGGAGAAGTTTCGAGGTCTTGTGGCTGTTCCTCAATTCAAAGGCAGAGGAGCTCCCGAAAGTGACACGGATTGGCGAGAAACCAATCATGGAGATGATATGAGAGGTGAAGAATGGTCTGATGTCGACGATGACGATGATGAATTCGACAACGATGGTGAGGATGACTGGATTGATGAACAGATCAATAACTTAGAAATGGCTGATCAGAGGCTTCTTTCTCCTCCTGCATTACCCGATGGACGGCCCCAAGAAAGATGGTGA

mRNA sequence

ATGTTCGCACATTTCATTCTTCCGCACAGAGCTTACTTTTCTGTTAAATCTGAATTTTGGGGTAAGAATTTGGATTTTCGGAACAGAAATGATAGTTTTAGTGATAATTCAAGAAAATCTCATGTGTATTTTCAACCGATTAGAGCTGGTGTGAAATGGAGGAAGGAGCTTCCGTTTGATAATGTAATACAGAGGGATAAGAAGCTGAAATTGGTTACTCGGATTAGGAAGATTCTAGTTCAGCATCATGATAATGTTATGTCACTTAGGGGTTTGGGTAAATTCAGGAGAGATTTAGGTGTACAGGAAAAGACGAGGCTAATTGAGTTGTTGAAGATGTTCCCTGCAGTATTTGAGATTGTAGAAGAAGAAGCGTTTTCTTTGAAATTTAAATTGACGGAAGAGGCTGAAAGGTTGTACTTCGAGGAGCTGAAGATCAGAAATGAAATGGAAGGTTTGTTGGTTACTAAGCTGAGGAAGTTACTGATGATGTCTGCTGATAAGCGAATATTGTTGGAGAAAATTGCACATTTGAGGACTGATTTTGGTCTTCCTTCCGAGTTTCGTGACACGATTTGCCATCGTTATCCTCAGTACTTTAGAGTTGTGGCAACCGAGAGCGGTCCAGCTCTCGAATTGACACACTGGGATCCTGAACTTGCTGTTTCTGCAGCAGAGTTAGCCGAGGAGGAGAACCGAGCTAGAGAATTGGAAGAGAAGAATTTGATTATTGACCGACCGCTGAAGTTTAATCGAGTGAAGCTACCTAAGGGGCTTAAGCTTTCGAAGAATGAGATGAGGAAAATGAATGAGTTTAGGGACATTCCTTATATCTCTCCGTATTCTGATTTCTCAGGGATTAAGGCAGGTACACCCCAGAAAGAGAAACATGCTTGTGGGGTTGTTCATGAGATTTTAAGCCTTACACTCGAGAAGAGAACTCTTGTTGATCATTTCACTCATTTCCGGGAGGAGTTTAGATTCTCCCAGCAGTTGAGGGGGATGCTAATAAGGCATCCCAATATGTTTTATGTCTCGTTGAAAGGGGATAAAAACTCGGTTTTCCTCAGAGAAGCATATCGCGATTCTCAGTTGATCGACAAAGATCGGTTGTTAATCATAAAGGAGAAGTTTCGAGGTCTTGTGGCTGTTCCTCAATTCAAAGGCAGAGGAGCTCCCGAAAGTGACACGGATTGGCGAGAAACCAATCATGGAGATGATATGAGAGGTGAAGAATGGTCTGATGTCGACGATGACGATGATGAATTCGACAACGATGGTGAGGATGACTGGATTGATGAACAGATCAATAACTTAGAAATGGCTGATCAGAGGCTTCTTTCTCCTCCTGCATTACCCGATGGACGGCCCCAAGAAAGATGGTGA

Coding sequence (CDS)

ATGTTCGCACATTTCATTCTTCCGCACAGAGCTTACTTTTCTGTTAAATCTGAATTTTGGGGTAAGAATTTGGATTTTCGGAACAGAAATGATAGTTTTAGTGATAATTCAAGAAAATCTCATGTGTATTTTCAACCGATTAGAGCTGGTGTGAAATGGAGGAAGGAGCTTCCGTTTGATAATGTAATACAGAGGGATAAGAAGCTGAAATTGGTTACTCGGATTAGGAAGATTCTAGTTCAGCATCATGATAATGTTATGTCACTTAGGGGTTTGGGTAAATTCAGGAGAGATTTAGGTGTACAGGAAAAGACGAGGCTAATTGAGTTGTTGAAGATGTTCCCTGCAGTATTTGAGATTGTAGAAGAAGAAGCGTTTTCTTTGAAATTTAAATTGACGGAAGAGGCTGAAAGGTTGTACTTCGAGGAGCTGAAGATCAGAAATGAAATGGAAGGTTTGTTGGTTACTAAGCTGAGGAAGTTACTGATGATGTCTGCTGATAAGCGAATATTGTTGGAGAAAATTGCACATTTGAGGACTGATTTTGGTCTTCCTTCCGAGTTTCGTGACACGATTTGCCATCGTTATCCTCAGTACTTTAGAGTTGTGGCAACCGAGAGCGGTCCAGCTCTCGAATTGACACACTGGGATCCTGAACTTGCTGTTTCTGCAGCAGAGTTAGCCGAGGAGGAGAACCGAGCTAGAGAATTGGAAGAGAAGAATTTGATTATTGACCGACCGCTGAAGTTTAATCGAGTGAAGCTACCTAAGGGGCTTAAGCTTTCGAAGAATGAGATGAGGAAAATGAATGAGTTTAGGGACATTCCTTATATCTCTCCGTATTCTGATTTCTCAGGGATTAAGGCAGGTACACCCCAGAAAGAGAAACATGCTTGTGGGGTTGTTCATGAGATTTTAAGCCTTACACTCGAGAAGAGAACTCTTGTTGATCATTTCACTCATTTCCGGGAGGAGTTTAGATTCTCCCAGCAGTTGAGGGGGATGCTAATAAGGCATCCCAATATGTTTTATGTCTCGTTGAAAGGGGATAAAAACTCGGTTTTCCTCAGAGAAGCATATCGCGATTCTCAGTTGATCGACAAAGATCGGTTGTTAATCATAAAGGAGAAGTTTCGAGGTCTTGTGGCTGTTCCTCAATTCAAAGGCAGAGGAGCTCCCGAAAGTGACACGGATTGGCGAGAAACCAATCATGGAGATGATATGAGAGGTGAAGAATGGTCTGATGTCGACGATGACGATGATGAATTCGACAACGATGGTGAGGATGACTGGATTGATGAACAGATCAATAACTTAGAAATGGCTGATCAGAGGCTTCTTTCTCCTCCTGCATTACCCGATGGACGGCCCCAAGAAAGATGGTGA

Protein sequence

MFAHFILPHRAYFSVKSEFWGKNLDFRNRNDSFSDNSRKSHVYFQPIRAGVKWRKELPFDNVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEIVEEEAFSLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRTDFGLPSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSAAELAEEENRARELEEKNLIIDRPLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGIKAGTPQKEKHACGVVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAYRDSQLIDKDRLLIIKEKFRGLVAVPQFKGRGAPESDTDWRETNHGDDMRGEEWSDVDDDDDEFDNDGEDDWIDEQINNLEMADQRLLSPPALPDGRPQERW
Homology
BLAST of Cp4.1LG12g04780 vs. ExPASy Swiss-Prot
Match: A0MFS5 (Protein WHAT'S THIS FACTOR 1 homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g01037 PE=3 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 1.4e-155
Identity = 299/497 (60.16%), Postives = 364/497 (73.24%), Query Frame = 0

Query: 7   LPHRAYFSVKSEFWGKNLDFRNRNDSFSDNSRKSHVYFQPIRAGVKWRKELPFDNVIQRD 66
           LP     + +S+F G+ L   N+   F   SRK+ V  +P+RA VK RKEL FD+V+QRD
Sbjct: 33  LPMSTIRAARSQFMGEGLILGNKY-GFWSTSRKTRVVVEPVRAAVKRRKELTFDSVVQRD 92

Query: 67  KKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEIVEEEAF 126
           KKLKLV  IRKILV   D +MSLRGLGK+RRDLG++++ R I LL+ +P VFEIVEE A+
Sbjct: 93  KKLKLVLNIRKILVSQPDRMMSLRGLGKYRRDLGLKKRRRFIALLRKYPGVFEIVEEGAY 152

Query: 127 SLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRTDFGLPS 186
           SL+FK+T EAERLY +E++IRNE+E +LV KLRKL+MMS DKRILLEKI+HL+TD GLP 
Sbjct: 153 SLRFKMTSEAERLYLDEMRIRNELEDVLVVKLRKLVMMSIDKRILLEKISHLKTDLGLPL 212

Query: 187 EFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSAAELAEEENRARELEEKNLIIDR 246
           EFRDTIC RYPQYFRVV T  GPALELTHWDPELAVSAAEL+E++NR RE EE+NLIIDR
Sbjct: 213 EFRDTICQRYPQYFRVVPTPRGPALELTHWDPELAVSAAELSEDDNRTRESEERNLIIDR 272

Query: 247 PLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGIKAGTPQKEKHACGVVHEIL 306
           P KFNRVKLP+GL LSK+E RK+++FRD+ YISPY DFS +++GT +KEKHACGV+HE+L
Sbjct: 273 PPKFNRVKLPRGLNLSKSETRKISQFRDMQYISPYKDFSHLRSGTLEKEKHACGVIHELL 332

Query: 307 SLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAYRDSQLI 366
           SLT EKRTLVDH THFREEFRFSQQLRGMLIRHP++FYVSLKG+++SVFLREAYR+S+LI
Sbjct: 333 SLTTEKRTLVDHLTHFREEFRFSQQLRGMLIRHPDLFYVSLKGERDSVFLREAYRNSELI 392

Query: 367 DKDRLLIIKEKFRGLVAVPQFKGRGAPESDTDWRETN-HGDDMRG---------EEWSDV 426
           DKD L ++KEK R LV+VP+F  RG P  D + RE    G D  G         EEWSDV
Sbjct: 393 DKDPLTLVKEKMRALVSVPRFPRRGGPRKDEEGREVEIDGSDADGEEEEEESDAEEWSDV 452

Query: 427 D---------DDDD--------------EFDNDGEDDWIDEQINNLEMADQRLLSP---- 462
           D         +DDD               FD+D ED+  D     L  + ++  SP    
Sbjct: 453 DGYLEGEDGGNDDDGDWTDDEGEEDVPPNFDDDDEDEEEDSVKIGLSPSSRKSSSPRKKE 512

BLAST of Cp4.1LG12g04780 vs. ExPASy Swiss-Prot
Match: Q65XL5 (Protein WHAT'S THIS FACTOR 1 homolog, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0571100 PE=2 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 6.5e-148
Identity = 284/453 (62.69%), Postives = 338/453 (74.61%), Query Frame = 0

Query: 48  RAGVKWRKELPFDNVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRL 107
           +A VK RKE+PFDNVIQRDKKLKLV ++R ILV + D VMSLR LG+FRRDLG+  K RL
Sbjct: 58  QAAVKRRKEIPFDNVIQRDKKLKLVLKLRNILVSNPDRVMSLRDLGRFRRDLGLTRKRRL 117

Query: 108 IELLKMFPAVFEIVEEEAFSLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSAD 167
           I LLK FP VFE+VEE  +SL+F+LT  AERLY +EL ++NE EGL VTKLRKLLMMS D
Sbjct: 118 IALLKRFPGVFEVVEEGVYSLRFRLTPAAERLYLDELHLKNESEGLAVTKLRKLLMMSQD 177

Query: 168 KRILLEKIAHLRTDFGLPSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSAAEL 227
           KRIL+EKIAHL+ D GLP EFRDTIC RYPQYFRVV  + GP LELTHWDPELAVSAAE+
Sbjct: 178 KRILIEKIAHLKNDLGLPPEFRDTICLRYPQYFRVVQMDRGPGLELTHWDPELAVSAAEV 237

Query: 228 AEEENRARELEEKNLIIDRPLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGI 287
           AEEENRARE +E+NLIIDRPLKFNRVKLP+GLKLS+ E R++ +F+++PYISPYSDFS +
Sbjct: 238 AEEENRAREEQERNLIIDRPLKFNRVKLPQGLKLSRGEARRVAQFKEMPYISPYSDFSHL 297

Query: 288 KAGTPQKEKHACGVVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSL 347
           ++G+ +KEKHACGVVHEILSLTLEKRTLVDH THFREEFRFSQ LRGMLIRHP+MFYVSL
Sbjct: 298 RSGSAEKEKHACGVVHEILSLTLEKRTLVDHLTHFREEFRFSQSLRGMLIRHPDMFYVSL 357

Query: 348 KGDKNSVFLREAYRDSQLIDKDRLLIIKEKFRGLVAVPQFKGRGAPESDTDWRETNHGDD 407
           KGD++SVFLREAY++SQL++K +L+++KEK R LVAVP+F  RG P +  +   TN    
Sbjct: 358 KGDRDSVFLREAYKNSQLVEKSKLVLLKEKMRALVAVPRFPRRGVPATSEEADRTNGAAQ 417

Query: 408 MRGEEWSDVDDDDDEFDNDGE------------------DDWI---DEQINNLEMADQRL 462
           M   E SDV+DD+DE  +D E                  D W+   D+   + E  D   
Sbjct: 418 ML-SEGSDVEDDEDEGLSDMEDLISEISGGKSDTDYHWGDGWVGENDDSPPDFEDDDGSS 477

BLAST of Cp4.1LG12g04780 vs. ExPASy Swiss-Prot
Match: B6TTV8 (Protein WHAT'S THIS FACTOR 1, chloroplastic OS=Zea mays OX=4577 GN=WTF1 PE=1 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 4.5e-141
Identity = 269/447 (60.18%), Postives = 333/447 (74.50%), Query Frame = 0

Query: 48  RAGVKWRKELPFDNVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRL 107
           +A VK RKE PFD VIQRDKKLKLV ++R ILV   D VMSLR LG+FRRDLG+  K RL
Sbjct: 52  QAAVKRRKEAPFDTVIQRDKKLKLVLKLRNILVAQPDRVMSLRELGRFRRDLGLTRKRRL 111

Query: 108 IELLKMFPAVFEIVEEEAFSLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSAD 167
           I LL+ FP VF++VEE  +SL+F+LT  AERLY +EL++RNE EGL V KLRKLLMMS +
Sbjct: 112 IALLRRFPGVFDVVEEGVYSLRFRLTPAAERLYLDELRLRNESEGLAVAKLRKLLMMSQE 171

Query: 168 KRILLEKIAHLRTDFGLPSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSAAEL 227
           KRIL+EK+AHL+ D GLP EFRDT+C RYPQYFRVV  + GPALELTHWDPELAVSAAEL
Sbjct: 172 KRILIEKVAHLKHDLGLPPEFRDTVCLRYPQYFRVVRMDRGPALELTHWDPELAVSAAEL 231

Query: 228 AEEENRARELEEKNLIIDRPLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGI 287
           AEEE+RARE EE+NLIIDRPLKFNRV+LPKGLKL++ E R++  F+++PYISPY+DFS +
Sbjct: 232 AEEESRAREAEERNLIIDRPLKFNRVRLPKGLKLTRGEARRIARFKEMPYISPYADFSHL 291

Query: 288 KAGTPQKEKHACGVVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSL 347
           ++G+ +KEKHACGVVHEILSLT+EKRTLVDH THFREEFRFSQ LRGM+IRHP+MFYVS 
Sbjct: 292 RSGSDEKEKHACGVVHEILSLTVEKRTLVDHLTHFREEFRFSQSLRGMIIRHPDMFYVSF 351

Query: 348 KGDKNSVFLREAYRDSQLIDKDRLLIIKEKFRGLVAVPQFKGRGAPESDTDWRETNHGDD 407
           KGD++SVFLREAY+DSQL++K++L+++KEK R LVAVP+F  R A  +  +    N    
Sbjct: 352 KGDRDSVFLREAYKDSQLVEKNQLVLLKEKMRALVAVPRFPRRAAVGTGEEAEGMNGSLQ 411

Query: 408 MRGEEWSDVDDDDDEFDNDGED-----------------------------DWIDEQIN- 462
            R +   +  DD+DE  +D ED                             D+ D+++  
Sbjct: 412 SRDQVSDEEYDDEDEGLSDMEDLISELSGGKSDADYEWGDGWFGENDDSPPDFGDDEVKV 471

BLAST of Cp4.1LG12g04780 vs. ExPASy Swiss-Prot
Match: Q689D6 (Protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=RPD1 PE=1 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 2.0e-35
Identity = 107/351 (30.48%), Postives = 188/351 (53.56%), Query Frame = 0

Query: 59  FDNVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLI-ELLKMFPAV 118
           +DN ++ +KK++ V +   +++   ++ +++  L    R LG+  K       L  FP V
Sbjct: 52  YDNYMEVEKKIRKVVKFHSLILSQPNHTIAISLLDTLARRLGLGFKQHEPGAFLLKFPHV 111

Query: 119 FEIVEEEAFSLKF-KLTEEAERLYFEELKIRNEMEGLL------VTKLRKLLMMSADKRI 178
           FEI E     + + +LT +A        +IR+E E +L      VT+LRKL+MMS   RI
Sbjct: 112 FEIYEHPVQRILYCRLTRKALD------QIRHEHEAVLDQIPDAVTRLRKLVMMSNTGRI 171

Query: 179 LLEKIAHLRTDFGLPSEFRDTICHRYPQYFRVVATES--GPALELTHWDPELAVSAAELA 238
            LE +   RT+FGLP +F  ++  ++PQ+FR++  E      +E+   DP L++ A E  
Sbjct: 172 RLEHVRIARTEFGLPEDFEYSVILKHPQFFRLIDGEETRDKYIEIVEKDPNLSICAIE-- 231

Query: 239 EEENRARELEEKNLIID-RPLKFN-RVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSG 298
               R RE+E +   ID   ++F+  V  P G K+ K     + +++ +PY SPY D SG
Sbjct: 232 ----RVREIEYRTKGIDAEDVRFSFVVNFPPGFKIGKYFRIAVWKWQRLPYWSPYEDISG 291

Query: 299 IKAGTPQK----EKHACGVVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNM 358
               + +     EK +   +HE+LSLT+EK+  ++   HFR      ++L+  L++H  +
Sbjct: 292 YDLRSMEAQNRLEKRSVACIHELLSLTVEKKITLERIAHFRNVMNLPKKLKEFLLQHQGI 351

Query: 359 FYVSLKGD---KNSVFLREAYRDSQLIDKDRLLIIKEKFRGLVAVPQFKGR 391
           FY+S +G+    ++VFLRE Y+  +L++ + + + + +   LV +   K +
Sbjct: 352 FYISTRGNYGKLHTVFLREGYKRGELVEPNDVYLARRRLAELVLMSPRKAK 390

BLAST of Cp4.1LG12g04780 vs. ExPASy Swiss-Prot
Match: Q9ZUZ6 (Protein WHAT'S THIS FACTOR 9, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=WTF9 PE=4 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.8e-25
Identity = 93/335 (27.76%), Postives = 158/335 (47.16%), Query Frame = 0

Query: 51  VKWRKELPFDNV--IQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLI 110
           +KW+++  FDN+  I R  +LK V  ++  +VQ  +  + +  + K  R   V   T++ 
Sbjct: 29  MKWKRDPYFDNIEHILRSSQLKSVVSLKNCIVQEPNRCIPISAISKKTRQFDV--STKIA 88

Query: 111 ELLKMFPAVFEIVEEEAFSLK-FKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSAD 170
             L+ FP++FE      ++L  F+LT EA  L  +E  +       L  +L+KL++MS D
Sbjct: 89  HFLRKFPSIFEEFVGPEYNLPWFRLTPEATELDRQERVVYQTSADDLRDRLKKLILMSKD 148

Query: 171 KRILLEKIAHLRTDFGLPSEFRDTICHRYPQYFRVVATESGP---ALELTHWDPELAVSA 230
             + L  +  ++   GLP ++           FR V  E G    A++    D  L+V  
Sbjct: 149 NVLPLSIVQGMKWYLGLPDDYLQFPDMNLDSSFRFVDMEDGVKGLAVDYNGGDKVLSVLQ 208

Query: 231 AELAEEENRARELEEKNLIIDRPLKFNRVKLP-KGLKLSKNEMRKMNEFRDIPYISPYSD 290
               ++      LEE    I+ PL       P KG +L       + EF+ +PY+SPY D
Sbjct: 209 KNAMKKRRGEVSLEE----IEFPL------FPSKGCRLRVKIEDWLMEFQKLPYVSPYDD 268

Query: 291 FSGIKAGTPQKEKHACGVVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMF 350
           +S +   +   EK   G +HE+L L +E           ++ F   Q++     RHP +F
Sbjct: 269 YSCLDPSSDIAEKRVVGFLHELLCLFVEHSAERKKLLCLKKHFGLPQKVHKAFERHPQIF 328

Query: 351 YVSLKGDKNSVFLREAYRDSQLIDKDRLLIIKEKF 379
           Y+S+K    +  LRE YRD   ++   +L +++K+
Sbjct: 329 YLSMKNKTCTAILREPYRDKASVETHPVLGVRKKY 351

BLAST of Cp4.1LG12g04780 vs. NCBI nr
Match: XP_023548741.1 (protein WHAT'S THIS FACTOR 1 homolog [Cucurbita pepo subsp. pepo] >XP_023548742.1 protein WHAT'S THIS FACTOR 1 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 914 bits (2363), Expect = 0.0
Identity = 461/461 (100.00%), Postives = 461/461 (100.00%), Query Frame = 0

Query: 1   MFAHFILPHRAYFSVKSEFWGKNLDFRNRNDSFSDNSRKSHVYFQPIRAGVKWRKELPFD 60
           MFAHFILPHRAYFSVKSEFWGKNLDFRNRNDSFSDNSRKSHVYFQPIRAGVKWRKELPFD
Sbjct: 1   MFAHFILPHRAYFSVKSEFWGKNLDFRNRNDSFSDNSRKSHVYFQPIRAGVKWRKELPFD 60

Query: 61  NVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEI 120
           NVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEI
Sbjct: 61  NVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEI 120

Query: 121 VEEEAFSLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRT 180
           VEEEAFSLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRT
Sbjct: 121 VEEEAFSLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRT 180

Query: 181 DFGLPSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSAAELAEEENRARELEEK 240
           DFGLPSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSAAELAEEENRARELEEK
Sbjct: 181 DFGLPSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSAAELAEEENRARELEEK 240

Query: 241 NLIIDRPLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGIKAGTPQKEKHACG 300
           NLIIDRPLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGIKAGTPQKEKHACG
Sbjct: 241 NLIIDRPLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGIKAGTPQKEKHACG 300

Query: 301 VVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAY 360
           VVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAY
Sbjct: 301 VVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAY 360

Query: 361 RDSQLIDKDRLLIIKEKFRGLVAVPQFKGRGAPESDTDWRETNHGDDMRGEEWSDVDDDD 420
           RDSQLIDKDRLLIIKEKFRGLVAVPQFKGRGAPESDTDWRETNHGDDMRGEEWSDVDDDD
Sbjct: 361 RDSQLIDKDRLLIIKEKFRGLVAVPQFKGRGAPESDTDWRETNHGDDMRGEEWSDVDDDD 420

Query: 421 DEFDNDGEDDWIDEQINNLEMADQRLLSPPALPDGRPQERW 461
           DEFDNDGEDDWIDEQINNLEMADQRLLSPPALPDGRPQERW
Sbjct: 421 DEFDNDGEDDWIDEQINNLEMADQRLLSPPALPDGRPQERW 461

BLAST of Cp4.1LG12g04780 vs. NCBI nr
Match: XP_022929851.1 (protein WHAT'S THIS FACTOR 1 homolog [Cucurbita moschata] >XP_022929852.1 protein WHAT'S THIS FACTOR 1 homolog [Cucurbita moschata] >XP_022929853.1 protein WHAT'S THIS FACTOR 1 homolog [Cucurbita moschata] >XP_022929854.1 protein WHAT'S THIS FACTOR 1 homolog [Cucurbita moschata])

HSP 1 Score: 899 bits (2324), Expect = 0.0
Identity = 453/461 (98.26%), Postives = 455/461 (98.70%), Query Frame = 0

Query: 1   MFAHFILPHRAYFSVKSEFWGKNLDFRNRNDSFSDNSRKSHVYFQPIRAGVKWRKELPFD 60
           MFAHFILPHRAYFSVKSEFWGKNLDFRNRNDS SDNSRKSHVYFQPIRAGVKWRKELPFD
Sbjct: 1   MFAHFILPHRAYFSVKSEFWGKNLDFRNRNDSLSDNSRKSHVYFQPIRAGVKWRKELPFD 60

Query: 61  NVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEI 120
           NVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEI
Sbjct: 61  NVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEI 120

Query: 121 VEEEAFSLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRT 180
           VEE AFSLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRT
Sbjct: 121 VEERAFSLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRT 180

Query: 181 DFGLPSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSAAELAEEENRARELEEK 240
           DFGLPSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVS AELAEEENRARELEEK
Sbjct: 181 DFGLPSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSVAELAEEENRARELEEK 240

Query: 241 NLIIDRPLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGIKAGTPQKEKHACG 300
           NLIIDRPLKFNRVKLPKGLKLSKN+MRKMNEFRDIPYISPYSDFSG KAGTPQKEKHACG
Sbjct: 241 NLIIDRPLKFNRVKLPKGLKLSKNDMRKMNEFRDIPYISPYSDFSGFKAGTPQKEKHACG 300

Query: 301 VVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAY 360
           VVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAY
Sbjct: 301 VVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAY 360

Query: 361 RDSQLIDKDRLLIIKEKFRGLVAVPQFKGRGAPESDTDWRETNHGDDMRGEEWSDVDDDD 420
           RDSQLIDKDRLLI+KEKFRGLVAVPQFKGRGAPESDTDWRETNHGDDMRGEEWSDVDDDD
Sbjct: 361 RDSQLIDKDRLLIVKEKFRGLVAVPQFKGRGAPESDTDWRETNHGDDMRGEEWSDVDDDD 420

Query: 421 DEFDNDGEDDWIDEQINNLEMADQRLLSPPALPDGRPQERW 461
           DEFDNDGEDDWIDEQINNLEMADQRLLSPPALP G PQERW
Sbjct: 421 DEFDNDGEDDWIDEQINNLEMADQRLLSPPALPYGLPQERW 461

BLAST of Cp4.1LG12g04780 vs. NCBI nr
Match: KAG7013857.1 (Protein ROOT PRIMORDIUM DEFECTIVE 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 899 bits (2322), Expect = 0.0
Identity = 451/461 (97.83%), Postives = 458/461 (99.35%), Query Frame = 0

Query: 1   MFAHFILPHRAYFSVKSEFWGKNLDFRNRNDSFSDNSRKSHVYFQPIRAGVKWRKELPFD 60
           MFAHFILPHR YFSVKSEFWGKNLDFRNRNDSFSDNSRKSHVYFQPIRAGVKWRKELPFD
Sbjct: 1   MFAHFILPHRPYFSVKSEFWGKNLDFRNRNDSFSDNSRKSHVYFQPIRAGVKWRKELPFD 60

Query: 61  NVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEI 120
           NVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEI
Sbjct: 61  NVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEI 120

Query: 121 VEEEAFSLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRT 180
           VEEEAFSLKFKLTEEAERLYFEE+KIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRT
Sbjct: 121 VEEEAFSLKFKLTEEAERLYFEEMKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRT 180

Query: 181 DFGLPSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSAAELAEEENRARELEEK 240
           DFGLPSEFRDTICHRYPQYFRVVATE GPALELTHWDPELAVSAAELA+EENRARELEEK
Sbjct: 181 DFGLPSEFRDTICHRYPQYFRVVATEHGPALELTHWDPELAVSAAELADEENRARELEEK 240

Query: 241 NLIIDRPLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGIKAGTPQKEKHACG 300
           NLIIDRPLKFNRVKLPKGLKLSKN+MRKMNEFRDIPYISPYSDFSGIKAGTPQKEKHACG
Sbjct: 241 NLIIDRPLKFNRVKLPKGLKLSKNDMRKMNEFRDIPYISPYSDFSGIKAGTPQKEKHACG 300

Query: 301 VVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAY 360
           VVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAY
Sbjct: 301 VVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAY 360

Query: 361 RDSQLIDKDRLLIIKEKFRGLVAVPQFKGRGAPESDTDWRETNHGDDMRGEEWSDVDDDD 420
           RDSQLIDKDRLLI+KEKFRGLVAVPQFKGRGAP+SDT+W+ETNHGDDMRGEEWSDVDDDD
Sbjct: 361 RDSQLIDKDRLLIVKEKFRGLVAVPQFKGRGAPKSDTNWQETNHGDDMRGEEWSDVDDDD 420

Query: 421 DEFDNDGEDDWIDEQINNLEMADQRLLSPPALPDGRPQERW 461
           DEFDNDGEDDWIDEQINNLEMADQRLLSP ALPDGRPQERW
Sbjct: 421 DEFDNDGEDDWIDEQINNLEMADQRLLSPHALPDGRPQERW 461

BLAST of Cp4.1LG12g04780 vs. NCBI nr
Match: XP_022992248.1 (protein WHAT'S THIS FACTOR 1 homolog [Cucurbita maxima] >XP_022992249.1 protein WHAT'S THIS FACTOR 1 homolog [Cucurbita maxima] >XP_022992250.1 protein WHAT'S THIS FACTOR 1 homolog [Cucurbita maxima] >XP_022992251.1 protein WHAT'S THIS FACTOR 1 homolog [Cucurbita maxima])

HSP 1 Score: 897 bits (2317), Expect = 0.0
Identity = 450/461 (97.61%), Postives = 456/461 (98.92%), Query Frame = 0

Query: 1   MFAHFILPHRAYFSVKSEFWGKNLDFRNRNDSFSDNSRKSHVYFQPIRAGVKWRKELPFD 60
           MFAHFILPHR YFSVKSEFWGKNLDFRNRNDS SDNSRK HVYFQPIRAGVKWRKELPFD
Sbjct: 1   MFAHFILPHRPYFSVKSEFWGKNLDFRNRNDSLSDNSRKYHVYFQPIRAGVKWRKELPFD 60

Query: 61  NVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEI 120
           NVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEI
Sbjct: 61  NVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEI 120

Query: 121 VEEEAFSLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRT 180
           VEEEAFSLKFKLTEEAERLYFEE+KIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRT
Sbjct: 121 VEEEAFSLKFKLTEEAERLYFEEMKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRT 180

Query: 181 DFGLPSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSAAELAEEENRARELEEK 240
           DFGLPSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSAAELA+EEN+ARELEEK
Sbjct: 181 DFGLPSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSAAELADEENQARELEEK 240

Query: 241 NLIIDRPLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGIKAGTPQKEKHACG 300
           NLIIDRPLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGI AGTPQKEKHACG
Sbjct: 241 NLIIDRPLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGITAGTPQKEKHACG 300

Query: 301 VVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAY 360
           VVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAY
Sbjct: 301 VVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAY 360

Query: 361 RDSQLIDKDRLLIIKEKFRGLVAVPQFKGRGAPESDTDWRETNHGDDMRGEEWSDVDDDD 420
           RDSQLIDKDRLLI+KEKFRGLVAVPQ KGRGAP+SDTDWRETNHGDDMRGEEWSDVDDDD
Sbjct: 361 RDSQLIDKDRLLIVKEKFRGLVAVPQLKGRGAPKSDTDWRETNHGDDMRGEEWSDVDDDD 420

Query: 421 DEFDNDGEDDWIDEQINNLEMADQRLLSPPALPDGRPQERW 461
           DEFDNDGEDDWIDEQINNL+MADQRLLSPPALPDGRPQERW
Sbjct: 421 DEFDNDGEDDWIDEQINNLDMADQRLLSPPALPDGRPQERW 461

BLAST of Cp4.1LG12g04780 vs. NCBI nr
Match: KAG6575319.1 (Protein WHAT'S THIS FACTOR 1-like, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 894 bits (2309), Expect = 0.0
Identity = 449/461 (97.40%), Postives = 457/461 (99.13%), Query Frame = 0

Query: 1   MFAHFILPHRAYFSVKSEFWGKNLDFRNRNDSFSDNSRKSHVYFQPIRAGVKWRKELPFD 60
           MFAHFILPHR YFSVKSEFWGKNLDFRNRNDSFSDNSRKSHVYFQPIRAGVKWRKELPFD
Sbjct: 1   MFAHFILPHRPYFSVKSEFWGKNLDFRNRNDSFSDNSRKSHVYFQPIRAGVKWRKELPFD 60

Query: 61  NVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEI 120
           NVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEI
Sbjct: 61  NVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEI 120

Query: 121 VEEEAFSLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRT 180
           VEEEAFSLKFKLTEEAERLYFEE+KIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRT
Sbjct: 121 VEEEAFSLKFKLTEEAERLYFEEMKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRT 180

Query: 181 DFGLPSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSAAELAEEENRARELEEK 240
           DFGLPSEFRDTICHRYPQYFRVVATE GPALELTHWDPELAVSAAELA+EENRARELEEK
Sbjct: 181 DFGLPSEFRDTICHRYPQYFRVVATEHGPALELTHWDPELAVSAAELADEENRARELEEK 240

Query: 241 NLIIDRPLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGIKAGTPQKEKHACG 300
           NLIIDRPLKFNRVKLPKGLKLSKN+MRKMNEFRDIPYISPYSDFSGIKAGTPQKEKHACG
Sbjct: 241 NLIIDRPLKFNRVKLPKGLKLSKNDMRKMNEFRDIPYISPYSDFSGIKAGTPQKEKHACG 300

Query: 301 VVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAY 360
           VVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAY
Sbjct: 301 VVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAY 360

Query: 361 RDSQLIDKDRLLIIKEKFRGLVAVPQFKGRGAPESDTDWRETNHGDDMRGEEWSDVDDDD 420
           RDSQLIDKDRLLI+KEKFRGLVAVPQFKGRGAP+SDT+W+ETNHGDDMRGEEWSDVDDDD
Sbjct: 361 RDSQLIDKDRLLIVKEKFRGLVAVPQFKGRGAPKSDTNWQETNHGDDMRGEEWSDVDDDD 420

Query: 421 DEFDNDGEDDWIDEQINNLEMADQRLLSPPALPDGRPQERW 461
           DEFDNDGEDDWIDEQINNLEMADQRLLS  ALP+GRPQERW
Sbjct: 421 DEFDNDGEDDWIDEQINNLEMADQRLLSSHALPNGRPQERW 461

BLAST of Cp4.1LG12g04780 vs. ExPASy TrEMBL
Match: A0A6J1EPZ9 (protein WHAT'S THIS FACTOR 1 homolog OS=Cucurbita moschata OX=3662 GN=LOC111436333 PE=4 SV=1)

HSP 1 Score: 899 bits (2324), Expect = 0.0
Identity = 453/461 (98.26%), Postives = 455/461 (98.70%), Query Frame = 0

Query: 1   MFAHFILPHRAYFSVKSEFWGKNLDFRNRNDSFSDNSRKSHVYFQPIRAGVKWRKELPFD 60
           MFAHFILPHRAYFSVKSEFWGKNLDFRNRNDS SDNSRKSHVYFQPIRAGVKWRKELPFD
Sbjct: 1   MFAHFILPHRAYFSVKSEFWGKNLDFRNRNDSLSDNSRKSHVYFQPIRAGVKWRKELPFD 60

Query: 61  NVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEI 120
           NVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEI
Sbjct: 61  NVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEI 120

Query: 121 VEEEAFSLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRT 180
           VEE AFSLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRT
Sbjct: 121 VEERAFSLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRT 180

Query: 181 DFGLPSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSAAELAEEENRARELEEK 240
           DFGLPSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVS AELAEEENRARELEEK
Sbjct: 181 DFGLPSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSVAELAEEENRARELEEK 240

Query: 241 NLIIDRPLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGIKAGTPQKEKHACG 300
           NLIIDRPLKFNRVKLPKGLKLSKN+MRKMNEFRDIPYISPYSDFSG KAGTPQKEKHACG
Sbjct: 241 NLIIDRPLKFNRVKLPKGLKLSKNDMRKMNEFRDIPYISPYSDFSGFKAGTPQKEKHACG 300

Query: 301 VVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAY 360
           VVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAY
Sbjct: 301 VVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAY 360

Query: 361 RDSQLIDKDRLLIIKEKFRGLVAVPQFKGRGAPESDTDWRETNHGDDMRGEEWSDVDDDD 420
           RDSQLIDKDRLLI+KEKFRGLVAVPQFKGRGAPESDTDWRETNHGDDMRGEEWSDVDDDD
Sbjct: 361 RDSQLIDKDRLLIVKEKFRGLVAVPQFKGRGAPESDTDWRETNHGDDMRGEEWSDVDDDD 420

Query: 421 DEFDNDGEDDWIDEQINNLEMADQRLLSPPALPDGRPQERW 461
           DEFDNDGEDDWIDEQINNLEMADQRLLSPPALP G PQERW
Sbjct: 421 DEFDNDGEDDWIDEQINNLEMADQRLLSPPALPYGLPQERW 461

BLAST of Cp4.1LG12g04780 vs. ExPASy TrEMBL
Match: A0A6J1JV64 (protein WHAT'S THIS FACTOR 1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111488626 PE=4 SV=1)

HSP 1 Score: 897 bits (2317), Expect = 0.0
Identity = 450/461 (97.61%), Postives = 456/461 (98.92%), Query Frame = 0

Query: 1   MFAHFILPHRAYFSVKSEFWGKNLDFRNRNDSFSDNSRKSHVYFQPIRAGVKWRKELPFD 60
           MFAHFILPHR YFSVKSEFWGKNLDFRNRNDS SDNSRK HVYFQPIRAGVKWRKELPFD
Sbjct: 1   MFAHFILPHRPYFSVKSEFWGKNLDFRNRNDSLSDNSRKYHVYFQPIRAGVKWRKELPFD 60

Query: 61  NVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEI 120
           NVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEI
Sbjct: 61  NVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEI 120

Query: 121 VEEEAFSLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRT 180
           VEEEAFSLKFKLTEEAERLYFEE+KIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRT
Sbjct: 121 VEEEAFSLKFKLTEEAERLYFEEMKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRT 180

Query: 181 DFGLPSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSAAELAEEENRARELEEK 240
           DFGLPSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSAAELA+EEN+ARELEEK
Sbjct: 181 DFGLPSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSAAELADEENQARELEEK 240

Query: 241 NLIIDRPLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGIKAGTPQKEKHACG 300
           NLIIDRPLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGI AGTPQKEKHACG
Sbjct: 241 NLIIDRPLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGITAGTPQKEKHACG 300

Query: 301 VVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAY 360
           VVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAY
Sbjct: 301 VVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAY 360

Query: 361 RDSQLIDKDRLLIIKEKFRGLVAVPQFKGRGAPESDTDWRETNHGDDMRGEEWSDVDDDD 420
           RDSQLIDKDRLLI+KEKFRGLVAVPQ KGRGAP+SDTDWRETNHGDDMRGEEWSDVDDDD
Sbjct: 361 RDSQLIDKDRLLIVKEKFRGLVAVPQLKGRGAPKSDTDWRETNHGDDMRGEEWSDVDDDD 420

Query: 421 DEFDNDGEDDWIDEQINNLEMADQRLLSPPALPDGRPQERW 461
           DEFDNDGEDDWIDEQINNL+MADQRLLSPPALPDGRPQERW
Sbjct: 421 DEFDNDGEDDWIDEQINNLDMADQRLLSPPALPDGRPQERW 461

BLAST of Cp4.1LG12g04780 vs. ExPASy TrEMBL
Match: A0A1S3CG79 (protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Cucumis melo OX=3656 GN=LOC103500096 PE=4 SV=1)

HSP 1 Score: 707 bits (1825), Expect = 6.35e-253
Identity = 382/494 (77.33%), Postives = 408/494 (82.59%), Query Frame = 0

Query: 5   FILPHRAYFSVKSEFWGKNLDFRNRNDSFSDNSRKSHVYFQPIRAGVKWRKELPFDNVIQ 64
           FIL H++Y+SVKS+FWGKNLD RNRND    N RK+H  FQPIRA VK RKELPFDNVIQ
Sbjct: 20  FILGHKSYYSVKSQFWGKNLDLRNRND----NLRKAHDPFQPIRAVVKRRKELPFDNVIQ 79

Query: 65  RDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEIVEEE 124
           RDKKLKLV RIRKILVQ  D VMSL+ LGKFRRDLG+++K RLI LLK FPAVFE+VEE 
Sbjct: 80  RDKKLKLVMRIRKILVQQPDRVMSLKELGKFRRDLGLEKKRRLIALLKKFPAVFEVVEEG 139

Query: 125 AFSLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRTDFGL 184
           AFSLKFKLT EAERLY EELKIRNEMEGLLV KLRKLLMMSADKRILLEKIAHLRTDFGL
Sbjct: 140 AFSLKFKLTAEAERLYLEELKIRNEMEGLLVIKLRKLLMMSADKRILLEKIAHLRTDFGL 199

Query: 185 PSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSAAELAEEENRARELEEKNLII 244
           P EFRDTICHRYPQYFRVVATE GPALELTHWDPELAVSAAELAEEENRARELEEKNLII
Sbjct: 200 PLEFRDTICHRYPQYFRVVATERGPALELTHWDPELAVSAAELAEEENRARELEEKNLII 259

Query: 245 DRPLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGIKAGTPQKEKHACGVVHE 304
           DRPLKFNRVKLPKGL LSK+EMRK+++FRDIPYISPYSDFSGIKAGTPQKEKHACGVVHE
Sbjct: 260 DRPLKFNRVKLPKGLNLSKSEMRKISQFRDIPYISPYSDFSGIKAGTPQKEKHACGVVHE 319

Query: 305 ILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAYRDSQ 364
           IL+LTLEKRTLVDH THFREEFRFSQQLRGMLIRHP+MFYVSLKGD++SVFLREAYRDSQ
Sbjct: 320 ILNLTLEKRTLVDHLTHFREEFRFSQQLRGMLIRHPDMFYVSLKGDRDSVFLREAYRDSQ 379

Query: 365 LIDKDRLLIIKEKFRGLVAVPQFKGRGAPESDTDWRETNHGDDMRGEEWSDVD----DDD 424
           LIDKDRLLIIKEK R LVAVP+F+ RGA   DT   +TN   DM GEEWSDVD    DDD
Sbjct: 380 LIDKDRLLIIKEKLRALVAVPRFRSRGASNGDTKGGDTNQPSDMSGEEWSDVDNLLDDDD 439

Query: 425 DEFDNDG---------EDDWIDE------------------------QINNLEMADQRLL 461
           DEFD+D          EDDW DE                        Q+N+L+   Q  L
Sbjct: 440 DEFDDDEFDDDNNEAFEDDWSDEDDTPPSFNGDQDGESINIGSRKQKQVNDLQKVGQSRL 499

BLAST of Cp4.1LG12g04780 vs. ExPASy TrEMBL
Match: A0A5D3BY23 (Protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold675G00850 PE=4 SV=1)

HSP 1 Score: 705 bits (1819), Expect = 5.79e-252
Identity = 381/497 (76.66%), Postives = 408/497 (82.09%), Query Frame = 0

Query: 5   FILPHRAYFSVKSEFWGKNLDFRNRNDSFSDNSRKSHVYFQPIRAGVKWRKELPFDNVIQ 64
           FIL H++Y+SVKS+FWGKNLD RNRND    N RK+H  FQPIRA VK RKELPFDNVIQ
Sbjct: 20  FILGHKSYYSVKSQFWGKNLDLRNRND----NLRKAHDPFQPIRAVVKRRKELPFDNVIQ 79

Query: 65  RDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEIVEEE 124
           RDKKLKLV RIRKILVQ  D VMSL+ LGKFRRDLG+++K RLI LLK FPAVFE+VEE 
Sbjct: 80  RDKKLKLVMRIRKILVQQPDRVMSLKELGKFRRDLGLEKKRRLIALLKKFPAVFEVVEEG 139

Query: 125 AFSLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRTDFGL 184
           AFSLKFKLT EAERLY EELKIRNEMEGLLV KLRKLLMMSADKRILLEKIAHLRTDFGL
Sbjct: 140 AFSLKFKLTAEAERLYLEELKIRNEMEGLLVIKLRKLLMMSADKRILLEKIAHLRTDFGL 199

Query: 185 PSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSAAELAEEENRARELEEKNLII 244
           P EFRDTICHRYPQYFRVVATE GPALELTHWDPELAVSAAELAEEENRARELEEKNLII
Sbjct: 200 PLEFRDTICHRYPQYFRVVATERGPALELTHWDPELAVSAAELAEEENRARELEEKNLII 259

Query: 245 DRPLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGIKAGTPQKEKHACGVVHE 304
           DRPLKFNRVKLPKGL LSK+EMRK+++FRDIPYISPYSDFSGIKAGTPQKEKHACGVVHE
Sbjct: 260 DRPLKFNRVKLPKGLNLSKSEMRKISQFRDIPYISPYSDFSGIKAGTPQKEKHACGVVHE 319

Query: 305 ILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAYRDSQ 364
           IL+LTLEKRTLVDH THFREEFRFSQQLRGMLIRHP+MFYVSLKGD++SVFLREAYRDSQ
Sbjct: 320 ILNLTLEKRTLVDHLTHFREEFRFSQQLRGMLIRHPDMFYVSLKGDRDSVFLREAYRDSQ 379

Query: 365 LIDKDRLLIIKEKFRGLVAVPQFKGRGAPESDTDWRETNHGDDMRGEEWSDVDD------ 424
           LIDKDRLLIIKEK R LVAVP+F+ RGA   DT   +TN   DM GEEWSDVD+      
Sbjct: 380 LIDKDRLLIIKEKLRALVAVPRFRSRGASNGDTKGGDTNQPSDMSGEEWSDVDNLLDDDE 439

Query: 425 ------DDDEFDNDG----EDDWIDE------------------------QINNLEMADQ 461
                 DDDEFD+D     EDDW DE                        Q+N+L+   Q
Sbjct: 440 FDDDEFDDDEFDDDNNEAFEDDWSDEDDTPPSFNGDQDGESINIGSRKQKQVNDLQKVGQ 499

BLAST of Cp4.1LG12g04780 vs. ExPASy TrEMBL
Match: A0A6J1DTV5 (protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Momordica charantia OX=3673 GN=LOC111023444 PE=4 SV=1)

HSP 1 Score: 684 bits (1766), Expect = 7.18e-244
Identity = 372/495 (75.15%), Postives = 403/495 (81.41%), Query Frame = 0

Query: 5   FILPHRAYFS------VKSE-FWGKNLDFRNRNDSFSDNSRKSHVYFQPIRAGVKWRKEL 64
           FILP + YFS      +KSE FWG+NLD R+R DS S N RK HV  QPIRA VK RKEL
Sbjct: 20  FILPRKPYFSENPSFSLKSEEFWGRNLDLRHRIDSSSSNLRKFHVPLQPIRAVVKRRKEL 79

Query: 65  PFDNVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAV 124
            FDNVIQRDKKLKLV RIRKILVQ  D +MSL+ LGKFRRDLG+++K RLI LLK FPAV
Sbjct: 80  KFDNVIQRDKKLKLVMRIRKILVQRPDRIMSLKELGKFRRDLGLEKKRRLIALLKKFPAV 139

Query: 125 FEIVEEEAFSLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAH 184
           FE+VEE AFSLKFKLT EAERLY EELKIRNEMEGLLV KLRKLLMMS DKRILLEKIAH
Sbjct: 140 FEVVEEGAFSLKFKLTPEAERLYLEELKIRNEMEGLLVVKLRKLLMMSIDKRILLEKIAH 199

Query: 185 LRTDFGLPSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSAAELAEEENRAREL 244
           LRTDFGLP EFRDTICHRYP YFRVVAT  GPALELTHWDPELAVSAAELAEEENRAREL
Sbjct: 200 LRTDFGLPLEFRDTICHRYPHYFRVVATGRGPALELTHWDPELAVSAAELAEEENRAREL 259

Query: 245 EEKNLIIDRPLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGIKAGTPQKEKH 304
           EEKNLIIDRPLKFNRVKLPKGL LSK EMRK+++FRDIPY+SPYSDFS +KAGTPQKEKH
Sbjct: 260 EEKNLIIDRPLKFNRVKLPKGLNLSKGEMRKISQFRDIPYVSPYSDFSELKAGTPQKEKH 319

Query: 305 ACGVVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLR 364
           ACGVVHEILSLTLEKRTLVDH THFREEFRFSQQLRGMLIRHP+MFY+SLKGD++SVFLR
Sbjct: 320 ACGVVHEILSLTLEKRTLVDHLTHFREEFRFSQQLRGMLIRHPDMFYISLKGDRDSVFLR 379

Query: 365 EAYRDSQLIDKDRLLIIKEKFRGLVAVPQFKGRGAPESDTDWRETNHGDDMRGEEWSDVD 424
           EAYRDSQLIDKDRLLIIKEK R LVAVP+F+GRGA +SDTD  ETN  DD+ GE+WSDVD
Sbjct: 380 EAYRDSQLIDKDRLLIIKEKLRALVAVPRFRGRGATKSDTDGEETNQPDDLSGEDWSDVD 439

Query: 425 -----DDDDEFDNDG--EDDWIDE------------------------QINNLEMADQRL 461
                DDDDE D D   +D W DE                        +++NL+   Q L
Sbjct: 440 NLLGDDDDDEDDGDDTYDDGWSDEDDTPPSFDGDGDGEAINIVSSKQKRVDNLQKVGQSL 499

BLAST of Cp4.1LG12g04780 vs. TAIR 10
Match: AT4G01037.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 550.8 bits (1418), Expect = 1.0e-156
Identity = 299/497 (60.16%), Postives = 364/497 (73.24%), Query Frame = 0

Query: 7   LPHRAYFSVKSEFWGKNLDFRNRNDSFSDNSRKSHVYFQPIRAGVKWRKELPFDNVIQRD 66
           LP     + +S+F G+ L   N+   F   SRK+ V  +P+RA VK RKEL FD+V+QRD
Sbjct: 33  LPMSTIRAARSQFMGEGLILGNKY-GFWSTSRKTRVVVEPVRAAVKRRKELTFDSVVQRD 92

Query: 67  KKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEIVEEEAF 126
           KKLKLV  IRKILV   D +MSLRGLGK+RRDLG++++ R I LL+ +P VFEIVEE A+
Sbjct: 93  KKLKLVLNIRKILVSQPDRMMSLRGLGKYRRDLGLKKRRRFIALLRKYPGVFEIVEEGAY 152

Query: 127 SLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRTDFGLPS 186
           SL+FK+T EAERLY +E++IRNE+E +LV KLRKL+MMS DKRILLEKI+HL+TD GLP 
Sbjct: 153 SLRFKMTSEAERLYLDEMRIRNELEDVLVVKLRKLVMMSIDKRILLEKISHLKTDLGLPL 212

Query: 187 EFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSAAELAEEENRARELEEKNLIIDR 246
           EFRDTIC RYPQYFRVV T  GPALELTHWDPELAVSAAEL+E++NR RE EE+NLIIDR
Sbjct: 213 EFRDTICQRYPQYFRVVPTPRGPALELTHWDPELAVSAAELSEDDNRTRESEERNLIIDR 272

Query: 247 PLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGIKAGTPQKEKHACGVVHEIL 306
           P KFNRVKLP+GL LSK+E RK+++FRD+ YISPY DFS +++GT +KEKHACGV+HE+L
Sbjct: 273 PPKFNRVKLPRGLNLSKSETRKISQFRDMQYISPYKDFSHLRSGTLEKEKHACGVIHELL 332

Query: 307 SLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFLREAYRDSQLI 366
           SLT EKRTLVDH THFREEFRFSQQLRGMLIRHP++FYVSLKG+++SVFLREAYR+S+LI
Sbjct: 333 SLTTEKRTLVDHLTHFREEFRFSQQLRGMLIRHPDLFYVSLKGERDSVFLREAYRNSELI 392

Query: 367 DKDRLLIIKEKFRGLVAVPQFKGRGAPESDTDWRETN-HGDDMRG---------EEWSDV 426
           DKD L ++KEK R LV+VP+F  RG P  D + RE    G D  G         EEWSDV
Sbjct: 393 DKDPLTLVKEKMRALVSVPRFPRRGGPRKDEEGREVEIDGSDADGEEEEEESDAEEWSDV 452

Query: 427 D---------DDDD--------------EFDNDGEDDWIDEQINNLEMADQRLLSP---- 462
           D         +DDD               FD+D ED+  D     L  + ++  SP    
Sbjct: 453 DGYLEGEDGGNDDDGDWTDDEGEEDVPPNFDDDDEDEEEDSVKIGLSPSSRKSSSPRKKE 512

BLAST of Cp4.1LG12g04780 vs. TAIR 10
Match: AT5G62990.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 222.6 bits (566), Expect = 6.4e-58
Identity = 141/392 (35.97%), Postives = 220/392 (56.12%), Query Frame = 0

Query: 60  DNVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFE 119
           D  + +  +++ V ++  +L+    + + +  L K R  L ++    ++ +++ +P +FE
Sbjct: 66  DRHVVKQNRVRFVQKLNTLLLSKPKHYIPIEILYKCRSYLCIENPLAILSMIRRYPTIFE 125

Query: 120 I-----------VEEEAFSLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSADK 179
           +             +    L  +LT  A  L  +EL +++E+   L TKL+KLLM+S+ +
Sbjct: 126 LFTTPTPHLPMNATKPLSQLCVRLTSAASSLAMQELNLKSEISDKLATKLQKLLMLSSHR 185

Query: 180 RILLEKIAHLRTDFGLPSEFRDTICHRYPQYFRVVATESGPALELTHWDPELAVSAAELA 239
           R+LL K+ H+  DFG P  FR  +C+ YP  F+ V T  G ALEL   DPELA       
Sbjct: 186 RLLLSKLVHIAPDFGFPPNFRSRLCNDYPDKFKTVDTSYGRALELVSSDPELA------- 245

Query: 240 EEENRARELE-EKNLIIDRPLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGI 299
              N+    E ++ LI+DRPLKF R+ L +GL L +     + +FR+ P + PY   S  
Sbjct: 246 ---NQMPSPEVDRGLIVDRPLKFKRLNLRRGLNLKRRHQGFLIKFRESPDVCPYKMSSDY 305

Query: 300 KAG-TPQKEKHACGVVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVS 359
            A  + + EK AC VV E+L LT+EKRTL+DH THFR+EF    +LR +++RHP +FYVS
Sbjct: 306 LASESIEAEKRACAVVREVLGLTVEKRTLIDHLTHFRKEFSLPNKLRDLIVRHPELFYVS 365

Query: 360 LKGDKNSVFLREAYRDS-QLIDKDRLLIIKEKFRGLVAV-----PQFKGRGAPESDTDWR 419
           +KG ++SVFL EAY D+  L+DKD  L+I+E+   L+        + + +GA   D    
Sbjct: 366 IKGMRDSVFLVEAYNDNGDLLDKDERLVIRERLIDLIQEGKRIRRERRRKGATLGDKSVE 425

Query: 420 ETNHGDDMRGEEWSDVDDD-DDEFDN--DGED 430
           E    D+   +  SD+DD+ +D F+N  D ED
Sbjct: 426 EKRKDDETIDDYDSDLDDEYEDGFENLFDSED 447

BLAST of Cp4.1LG12g04780 vs. TAIR 10
Match: AT3G63090.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 195.7 bits (496), Expect = 8.4e-50
Identity = 129/339 (38.05%), Postives = 190/339 (56.05%), Query Frame = 0

Query: 51  VKWRKELPFDNVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIEL 110
           V W+K+   D  I++DK+ KL  R+ K ++     V+ LR L K R  L +  K +    
Sbjct: 36  VVWKKDTRLDEAIEQDKRYKLCARVVKEVLNEPGQVIPLRYLEKRRERLRLTFKAK--SF 95

Query: 111 LKMFPAVFEI----VEEEAFSLKF-KLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMS 170
           ++M P++FEI    ++ ++  ++F + T        EE +I +E E LLV+KL +LLMM+
Sbjct: 96  VEMNPSLFEIYYDRIKPKSDPVQFLRPTPRLRAFLDEEQRIYSENEPLLVSKLCQLLMMA 155

Query: 171 ADKRILLEKIAHLRTDFGLPSEFRDTICHRYPQYFRV--VATESGPALELTHWDPELAVS 230
            DK I  +K+ H++ DFG P++F   +  +YP YFR+  +  E    LEL  W+P+ A S
Sbjct: 156 KDKVISADKLVHVKRDFGFPNDFLMKLVQKYPNYFRLTGLPEEGKSFLELVSWNPDFAKS 215

Query: 231 AAELAEEENRARELEEKNLIIDRPLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSD 290
             EL     RA +   K  +  RP  +N VKLP G  L K       ++ +  YISPY D
Sbjct: 216 KIEL-----RAEDETLKTGVRIRP-NYN-VKLPSGFFLRKEMREWTRDWLEQDYISPYED 275

Query: 291 FSGIKAGTPQKEKHACGVVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMF 350
            S +   + + EK   GVVHE+LSL+L KR  V     F +EFRFS     +  RH  +F
Sbjct: 276 VSHLDQASKEMEKRTVGVVHELLSLSLLKRVPVPTLGKFCDEFRFSNAFSSVFTRHSGIF 335

Query: 351 YVSLKGDKNSVFLREAYRDSQLIDKDRLLIIKEKFRGLV 383
           Y+SLKG   +  LREAY+D +L+D+D LL IK+KF  L+
Sbjct: 336 YLSLKGGIKTAVLREAYKDDELVDRDPLLAIKDKFLRLL 365

BLAST of Cp4.1LG12g04780 vs. TAIR 10
Match: AT5G21970.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 176.4 bits (446), Expect = 5.3e-44
Identity = 139/450 (30.89%), Postives = 215/450 (47.78%), Query Frame = 0

Query: 6   ILPHRAYFSVKSEFWGKNLDFRNRNDSFSDNSRKSHVYFQPIRAGVKWRKELPFDNVIQR 65
           I+P R     +S F    + F   +    D SR+  V    I A  KW            
Sbjct: 22  IMPERNVLYGESSFRRVWVRFMTSSKRVQDRSREKRVQELEI-ATEKW------------ 81

Query: 66  DKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKMFPAVFEIVEEEA 125
            K    V  + ++L    D +M++R   ++RR + + +  ++ + ++  P +FE+ +++ 
Sbjct: 82  -KIASKVIFLMEVLKGERDMIMTVRSFEQYRRQINLPKPHKISDFIRKSPKLFELYKDQR 141

Query: 126 FSLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSADKRILLEKIAHLRTDFGLP 185
             L   LTE  E L  E  K+  E        + + LMMS DK++ L+KI H R DFGLP
Sbjct: 142 GVLWCGLTEGGEDLLDEHDKLLEENGDKAAEHVTRCLMMSVDKKLPLDKIVHFRRDFGLP 201

Query: 186 SEFRDTICHRYPQYFRVVATESGPA-LELTHWDPELAVSAAELAEEENRARELEEKNLII 245
            +FR    + +PQ+F+VV    G   LEL  W+P  A++            ELE+K L I
Sbjct: 202 LDFRINWVYNFPQHFKVVKLGDGEEYLELVSWNPAWAIT------------ELEKKTLGI 261

Query: 246 DRPLKFN--------RVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGIKAGTPQKEK 305
               +           +K P   K       K+  F+   Y+SPY+D  G++AG+ + +K
Sbjct: 262 TEDCEHKPGMLSLAFPMKFPPSYKKMYRYRGKIEHFQKRSYLSPYADARGLEAGSKEFDK 321

Query: 306 HACGVVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGDKNSVFL 365
            A  V+HE+LS TLEKR + DH THFR EF   Q+L  + ++H  +FYVS +G + SVFL
Sbjct: 322 RAIAVMHELLSFTLEKRLVTDHLTHFRREFVMPQKLMRIFLKHCGIFYVSERGKRFSVFL 381

Query: 366 REAYRDSQLIDKDRLLIIKEK------FRGLVAVPQFKGRGAPESDTDWRETNHGDD--- 425
            E Y   +LI+K  L++ KEK      +RG     Q         + +  E+  GD+   
Sbjct: 382 TEGYEGPELIEKCPLILWKEKLLKFTGYRGRKRDIQTYSDTLDMEERELLESGSGDEDLS 441

Query: 426 ---MRGEEWSDVDDDDDEFDNDGEDDWIDE 435
               +  ++ DV  DDDE D    +D  +E
Sbjct: 442 VGFEKDGDYDDVVTDDDEMDIGEVNDAYEE 445

BLAST of Cp4.1LG12g04780 vs. TAIR 10
Match: AT2G31290.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 165.6 bits (418), Expect = 9.3e-41
Identity = 106/332 (31.93%), Postives = 178/332 (53.61%), Query Frame = 0

Query: 54  RKELPFDNVIQRDKKLKLVTRIRKILVQHHDNVMSLRGLGKFRRDLGVQEKTRLIELLKM 113
           +K+   ++ + R+K+  + +R++ I+++  + V S++ L K  + L +Q K   +  LK 
Sbjct: 29  KKDPDLESALSRNKRWIVNSRLKNIILRCPNQVASIKFLQKKFKTLDLQGKA--LNWLKK 88

Query: 114 FPAVFEIVEEEAFSLKFKLTEEAERLYFEELKIRNEMEGLLVTKLRKLLMMSADKRILLE 173
           +P  F +  E       +LT+    L  EE  +++  E +L  +L KLLM+S ++R+ + 
Sbjct: 89  YPCCFHVYLEND-EYYCRLTKPMMTLVEEEELVKDTQEPVLADRLAKLLMLSVNQRLNVV 148

Query: 174 KIAHLRTDFGLPSEFRDTICHRYPQYFRVV---ATESGPALELTHWDPELAVSAAELAEE 233
           K+   +  FG P ++   I  +Y   FR+V     +S   +EL  W PELAVSA E A +
Sbjct: 149 KLNEFKRSFGFPDDYVIRIVPKYSDVFRLVNYSGRKSSMEIELLLWKPELAVSAVEAAAK 208

Query: 234 ENRARELEEKNLIIDRPLKFNRVKLPKGLKLSKNEMRKMNEFRDIPYISPYSDFSGIKAG 293
           +  +    E +     P  + +               +  EF   PYISPY D   +  G
Sbjct: 209 KCGS----EPSFSCSLPSTWTK------------PWERFMEFNAFPYISPYEDHGDLVEG 268

Query: 294 TPQKEKHACGVVHEILSLTLEKRTLVDHFTHFREEFRFSQQLRGMLIRHPNMFYVSLKGD 353
           + + EK + G+VHE+LSLTL K+  +   +HF+ EF   ++L GML++HP +FYV  K  
Sbjct: 269 SKESEKRSVGLVHELLSLTLWKKLSIVKLSHFKREFGLPEKLNGMLLKHPGIFYVGNKYQ 328

Query: 354 KNSVFLREAYRDSQLIDKDRLLIIKEKFRGLV 383
            ++V LRE Y  S+LI KD L+++K+KF  L+
Sbjct: 329 VHTVILREGYNGSELIHKDPLVVVKDKFGELM 341

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0MFS51.4e-15560.16Protein WHAT'S THIS FACTOR 1 homolog, chloroplastic OS=Arabidopsis thaliana OX=3... [more]
Q65XL56.5e-14862.69Protein WHAT'S THIS FACTOR 1 homolog, chloroplastic OS=Oryza sativa subsp. japon... [more]
B6TTV84.5e-14160.18Protein WHAT'S THIS FACTOR 1, chloroplastic OS=Zea mays OX=4577 GN=WTF1 PE=1 SV=... [more]
Q689D62.0e-3530.48Protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=RPD1 PE=1... [more]
Q9ZUZ61.8e-2527.76Protein WHAT'S THIS FACTOR 9, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=W... [more]
Match NameE-valueIdentityDescription
XP_023548741.10.0100.00protein WHAT'S THIS FACTOR 1 homolog [Cucurbita pepo subsp. pepo] >XP_023548742.... [more]
XP_022929851.10.098.26protein WHAT'S THIS FACTOR 1 homolog [Cucurbita moschata] >XP_022929852.1 protei... [more]
KAG7013857.10.097.83Protein ROOT PRIMORDIUM DEFECTIVE 1, partial [Cucurbita argyrosperma subsp. argy... [more]
XP_022992248.10.097.61protein WHAT'S THIS FACTOR 1 homolog [Cucurbita maxima] >XP_022992249.1 protein ... [more]
KAG6575319.10.097.40Protein WHAT'S THIS FACTOR 1-like, chloroplastic, partial [Cucurbita argyrosperm... [more]
Match NameE-valueIdentityDescription
A0A6J1EPZ90.098.26protein WHAT'S THIS FACTOR 1 homolog OS=Cucurbita moschata OX=3662 GN=LOC1114363... [more]
A0A6J1JV640.097.61protein WHAT'S THIS FACTOR 1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111488626... [more]
A0A1S3CG796.35e-25377.33protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Cucumis melo OX=3656 GN=LOC103500096 PE=4... [more]
A0A5D3BY235.79e-25276.66Protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A6J1DTV57.18e-24475.15protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Momordica charantia OX=3673 GN=LOC1110234... [more]
Match NameE-valueIdentityDescription
AT4G01037.11.0e-15660.16Ubiquitin carboxyl-terminal hydrolase family protein [more]
AT5G62990.16.4e-5835.97Ubiquitin carboxyl-terminal hydrolase family protein [more]
AT3G63090.18.4e-5038.05Ubiquitin carboxyl-terminal hydrolase family protein [more]
AT5G21970.15.3e-4430.89Ubiquitin carboxyl-terminal hydrolase family protein [more]
AT2G31290.19.3e-4131.93Ubiquitin carboxyl-terminal hydrolase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 220..240
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 389..461
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 393..411
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 412..436
IPR021099Plant organelle RNA recognition domainPFAMPF11955PORRcoord: 55..383
e-value: 1.2E-106
score: 356.3
IPR045040PORR familyPANTHERPTHR31476PROTEIN WHAT'S THIS FACTOR 1 HOMOLOG, CHLOROPLASTICcoord: 18..435
IPR040293Protein WHAT'S THIS FACTOR 1-likePANTHERPTHR31476:SF4PROTEIN WHAT'S THIS FACTOR 1 HOMOLOG, CHLOROPLASTICcoord: 18..435

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG12g04780.1Cp4.1LG12g04780.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000373 Group II intron splicing
molecular_function GO:0003723 RNA binding