Cp4.1LG12g03990 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG12g03990 vs. ExPASy Swiss-Prot
Match: Q8VYU6 (Golgin candidate 4 OS=Arabidopsis thaliana OX=3702 GN=GC4 PE=2 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 1.3e-156
Identity = 384/811 (47.35%), Postives = 500/811 (61.65%), Query Frame = 0

Query: 27  NSIANLKENLNKIALDVHHD--DDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRS 86
           +S+ANLKENLNKIA DVH D  DD+E+  IYGS  G      +DRRNS+ F +S    RS
Sbjct: 3   SSVANLKENLNKIAHDVHDDDEDDDEDLTIYGSTNGG-----TDRRNSNGFRYS----RS 62

Query: 87  PIANGIEDAHHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKE-----------SLE 146
           P+ANG E   +PEIE+YK EI +LQ+SE +IK+LS+NYAALLKEKE           SL+
Sbjct: 63  PMANGFESPVNPEIERYKAEINKLQKSESEIKALSVNYAALLKEKEDQISRLNQENGSLK 122

Query: 147 GTNTSTNSPRAES--SKSPSNGTNEMKGS-DQSPTRLLRGKTR-------RNGIVSKQDG 206
              TSTN+   ES    S ++  N +KG+ D SP R  R  T         NGI SK +G
Sbjct: 123 QNLTSTNAALKESRLDLSRASNNNAIKGNGDHSPNRSQRSPTNWKNRNQMNNGIASKPNG 182

Query: 207 IANGASHSGKLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQERDQ 266
             N +               H   +E  +  E    S+   Q         +EL++ER++
Sbjct: 183 TENDSE-------------SHKKEKEFAEMLEERTRSMASAQA--------RELEKEREK 242

Query: 267 LADVQLRLREEQKLNKKFQEELNSLHMNKDKASLEMSNIIRELNEKKLEVKQLQVELNRR 326
            A++Q+ L+EE+K N+ F+EEL SL ++K+K  +E + + REL+ K  E++QLQ++LN  
Sbjct: 243 SANLQILLQEERKQNETFKEELQSLRLDKEKTLMESNKVRRELDAKLAEIRQLQMKLNGG 302

Query: 327 ENMKSDDNVEGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSLEMVNRHL 386
           E      + E LK +   LEKE + L++ + ELE  LE  + S+S +    S E ++RHL
Sbjct: 303 EQHAFGISRENLKEVNKALEKENNELKLKRSELEAALEASQKSTSRKLFPKSTEDLSRHL 362

Query: 387 SGSNEASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNVEGLKRLITKLEKEKSTL 446
           S                                                   L++EK   
Sbjct: 363 S--------------------------------------------------SLDEEK--- 422

Query: 447 EMGKKELEDTLEKCRTSSSVEAQSSSLEMVNRHLSGSNEKLGLSGISPGKEDMDLSLQKL 506
                                                      +G  PGKEDM+ SLQ+L
Sbjct: 423 -------------------------------------------AGTFPGKEDMEKSLQRL 482

Query: 507 KKDLKEMQQERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHL 566
           +K+L+E ++E+DKA  EL RLKQHLLEKE+EESEKMDEDSR+I+ELR  NEYQR QIL L
Sbjct: 483 EKELEEARREKDKARQELKRLKQHLLEKETEESEKMDEDSRLIDELRQTNEYQRSQILGL 542

Query: 567 EKALNQAIATQKELEMYGKNELQKSKEIIEELNRKLANYTSIIDSKNVELLNLQTALGQY 626
           EKAL Q +A Q+E++     E++KSK IIE+LN+KLAN    IDSKNVELLNLQTALGQY
Sbjct: 543 EKALRQTMANQEEIKSSSDLEIRKSKGIIEDLNQKLANCLRTIDSKNVELLNLQTALGQY 602

Query: 627 YAEIEAKEHLESDLAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWK 686
           YAEIEAKEH E +LA  +E   KLS  LKD +++ ++ KKEKEEI SK+  +E    EWK
Sbjct: 603 YAEIEAKEHFERELAVAKEDAMKLSARLKDVDEQLESSKKEKEEITSKVLHAENIAAEWK 662

Query: 687 SRVNKLEEDNSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLM 746
           +RV+K+E+DN+K+RR L+QSMTRLNRMS+DSDFLVDRRIVIKLLVTYFQ+NHS+EVLDLM
Sbjct: 663 NRVSKVEDDNAKVRRVLEQSMTRLNRMSMDSDFLVDRRIVIKLLVTYFQRNHSREVLDLM 687

Query: 747 VRMLGFSEDDKMRIGAAKQGPS-KGVVRGVLGLPGRLVGGIL--GGSSAETPANMASDNQ 806
           VRMLGFSE++K RIG A+QG + KGVVRGVLG PGRLVGGIL  GG S ++  NMASDNQ
Sbjct: 723 VRMLGFSEEEKQRIGLAQQGAAGKGVVRGVLGFPGRLVGGILGGGGGSPDSHPNMASDNQ 687

Query: 807 SFADLWVDFLLKENEEREKREAKESLKLQEE 812
           SFAD+WV+FLLK+ EERE+REA+++   ++E
Sbjct: 783 SFADMWVEFLLKDAEERERREAEDAANKEQE 687

BLAST of Cp4.1LG12g03990 vs. ExPASy Swiss-Prot
Match: Q84WU4 (Golgin candidate 3 OS=Arabidopsis thaliana OX=3702 GN=GC3 PE=1 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 1.1e-155
Identity = 381/804 (47.39%), Postives = 495/804 (61.57%), Query Frame = 0

Query: 27  NSIANLKENLNKIALDVHHDDDEE-EFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSP 86
           +SI N+K NL+KI LDVH DD+EE +   YGS  G   VS SDRRNS  F     V+R  
Sbjct: 3   SSIENMKANLHKIVLDVHEDDEEEDDLHKYGSANG---VSNSDRRNSSGF---RSVSRYS 62

Query: 87  IANGIEDAHHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKE-----------SLEG 146
           I+NGIE   H EIE+YK EIK+LQESE DIK+LS+NYAALL+EKE           SL+ 
Sbjct: 63  ISNGIESPAHHEIERYKAEIKKLQESESDIKALSVNYAALLREKEDQISRLNQENGSLKQ 122

Query: 147 TNTSTNSPRAESSKSPSNGTNE--MKG-SDQSPTRLLRGKTRRNGIVSKQDGIANGASHS 206
             TST++   E+    S G+N   +KG +DQSP RL +  +     +   + ++NG    
Sbjct: 123 NLTSTSAALKEARTDISRGSNNYAIKGNNDQSPNRLHKSVSH----LKSPNHMSNG---- 182

Query: 207 GKLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQERDQLADVQLRL 266
                + K        ++L D  E    S+  VQ T        EL +ER++L D QL L
Sbjct: 183 -----KGKDTDSFIKEKDLADMLEDRTKSMAAVQAT--------ELAKEREKLRDFQLSL 242

Query: 267 REEQKLNKKFQEELNSLHMNKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDN 326
           +EE+K ++ F+EEL S+ ++K+K S+E+S +  EL+ K LE+K LQ++L  +E+      
Sbjct: 243 QEERKRSESFKEELESMRLDKNKTSMEISKMRSELDAKLLEIKHLQMKLTGQESHAIGPG 302

Query: 327 VEGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSLEMVNRHLSGSNEASL 386
           +E LK +   LEKE + L++ + ELE  LE+ R  ++ +    + E + RH         
Sbjct: 303 MEHLKEVNKALEKENNELKLKRSELEAALEESRKLTNSKVFPDATESLTRH--------- 362

Query: 387 EMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNVEGLKRLITKLEKEKSTLEMGKKELE 446
                                                            STL+  K E  
Sbjct: 363 ------------------------------------------------PSTLDKEKPE-- 422

Query: 447 DTLEKCRTSSSVEAQSSSLEMVNRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQ 506
                                                  PGKE+M+ SLQ+L+ DLKE Q
Sbjct: 423 -------------------------------------SFPGKEEMEQSLQRLEMDLKETQ 482

Query: 507 QERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAI 566
           +ERDKA  EL RLKQHLLEKE+EESEKMDEDSR+IEELR  NEYQR QI HLEK+L QAI
Sbjct: 483 RERDKARQELKRLKQHLLEKETEESEKMDEDSRLIEELRQTNEYQRSQISHLEKSLKQAI 542

Query: 567 ATQKELEMYGKNELQKSKEIIEELNRKLANYTSIIDSKNVELLNLQTALGQYYAEIEAKE 626
           + Q++  +   N+++K K+ +++LN+KL N    I+SKNVELLNLQTALGQYYAEIEAKE
Sbjct: 543 SNQEDNRLSNDNQIRKLKDTVDDLNQKLTNCLRTIESKNVELLNLQTALGQYYAEIEAKE 602

Query: 627 HLESDLAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWKSRVNKLEE 686
           H E +LA  ++   KLS  LKD+++R ++  KEKE++ SKL  +E+   EWK+RV K+EE
Sbjct: 603 HFERELAMAKDELMKLSARLKDSDERLESSNKEKEDVTSKLLHAEKVAAEWKNRVTKVEE 662

Query: 687 DNSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSE 746
           DN+K+RR L+QSMTRLNRMS++SD+LVDRRIVIKLLVTYFQKNH+KEVLDLMVRMLGFSE
Sbjct: 663 DNAKVRRVLEQSMTRLNRMSMESDYLVDRRIVIKLLVTYFQKNHNKEVLDLMVRMLGFSE 683

Query: 747 DDKMRIGAAKQGPSKGVVRGVLGLPGRLVGGILGGSSAETPANMASDNQSFADLWVDFLL 806
           +DK RIGAAKQG  KGVVRGVLG PGR VGGILGG SAE  AN ASDNQSFADLWVDFLL
Sbjct: 723 EDKERIGAAKQGGGKGVVRGVLGFPGRFVGGILGGKSAELHANAASDNQSFADLWVDFLL 683

Query: 807 KENEEREKREAKESL--KLQEESQ 814
           K+ EERE+REA+E+   K +++S+
Sbjct: 783 KDAEERERREAEEAAASKAKQDSE 683

BLAST of Cp4.1LG12g03990 vs. NCBI nr
Match: XP_023548091.1 (golgin candidate 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1352 bits (3500), Expect = 0.0
Identity = 765/874 (87.53%), Postives = 765/874 (87.53%), Query Frame = 0

Query: 28  SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA 87
           SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA
Sbjct: 5   SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA 64

Query: 88  NGIEDAHHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKE---------------SL 147
           NGIEDAHHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKE               SL
Sbjct: 65  NGIEDAHHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKEELILRLNKENGSLKQSL 124

Query: 148 EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGIANGASHSG 207
           EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGIANGASHSG
Sbjct: 125 EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGIANGASHSG 184

Query: 208 KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQERDQLADVQLRLR 267
           KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQERDQLADVQLRLR
Sbjct: 185 KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQERDQLADVQLRLR 244

Query: 268 EEQKLNKKFQEELNSLHMNKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNV 327
           EEQKLNKKFQEELNSLHMNKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNV
Sbjct: 245 EEQKLNKKFQEELNSLHMNKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNV 304

Query: 328 EGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSLEMVNRHLSGSNEASLE 387
           EGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSL                
Sbjct: 305 EGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSL---------------- 364

Query: 388 MSNIIRELNEKKLEVKQLQVELNRRENMKSDDNVEGLKRLITKLEKEKSTLEMGKKELED 447
                                                                       
Sbjct: 365 ------------------------------------------------------------ 424

Query: 448 TLEKCRTSSSVEAQSSSLEMVNRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQQ 507
                             EMVNRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQQ
Sbjct: 425 ------------------EMVNRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQQ 484

Query: 508 ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA 567
           ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA
Sbjct: 485 ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA 544

Query: 568 TQKELEMYGKNELQKSKEIIEELNRKLANYTSIIDSKNVELLNLQTALGQYYAEIEAKEH 627
           TQKELEMYGKNELQKSKEIIEELNRKLANYTSIIDSKNVELLNLQTALGQYYAEIEAKEH
Sbjct: 545 TQKELEMYGKNELQKSKEIIEELNRKLANYTSIIDSKNVELLNLQTALGQYYAEIEAKEH 604

Query: 628 LESDLAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWKSRVNKLEED 687
           LESDLAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWKSRVNKLEED
Sbjct: 605 LESDLAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWKSRVNKLEED 664

Query: 688 NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED 747
           NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED
Sbjct: 665 NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED 724

Query: 748 DKMRIGAAKQGPSKGVVRGVLGLPGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK 807
           DKMRIGAAKQGPSKGVVRGVLGLPGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK
Sbjct: 725 DKMRIGAAKQGPSKGVVRGVLGLPGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK 784

Query: 808 ENEEREKREAKESLKLQEESQLNGPNVGSIDPGTRATGSTSESSRTGFPSHHHHHHHHQS 867
           ENEEREKREAKESLKLQEESQLNGPNVGSIDPGTRATGSTSESSRTGFPSHHHHHHHHQS
Sbjct: 785 ENEEREKREAKESLKLQEESQLNGPNVGSIDPGTRATGSTSESSRTGFPSHHHHHHHHQS 784

Query: 868 THLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY 886
           THLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY
Sbjct: 845 THLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY 784

BLAST of Cp4.1LG12g03990 vs. NCBI nr
Match: KAG7013764.1 (Golgin candidate 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1320 bits (3416), Expect = 0.0
Identity = 754/879 (85.78%), Postives = 757/879 (86.12%), Query Frame = 0

Query: 28  SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA 87
           SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA
Sbjct: 5   SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA 64

Query: 88  NGIEDAHHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKE---------------SL 147
           NGIEDA HPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKE               SL
Sbjct: 65  NGIEDARHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKEELILRLNKENGSLKQSL 124

Query: 148 EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGIANGASHSG 207
           EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGI NGASHSG
Sbjct: 125 EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGITNGASHSG 184

Query: 208 KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQERDQLADVQLRLR 267
           KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQER+QLADVQLRLR
Sbjct: 185 KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQEREQLADVQLRLR 244

Query: 268 EEQKLNKKFQEELNSLHMNKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNV 327
           EEQKLNKKFQEELNSLHMNKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNV
Sbjct: 245 EEQKLNKKFQEELNSLHMNKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNV 304

Query: 328 EGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSLEMVNRHLSGSNEASLE 387
           EGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSL                
Sbjct: 305 EGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSL---------------- 364

Query: 388 MSNIIRELNEKKLEVKQLQVELNRRENMKSDDNVEGLKRLITKLEKEKSTLEMGKKELED 447
                                                                       
Sbjct: 365 ------------------------------------------------------------ 424

Query: 448 TLEKCRTSSSVEAQSSSLEMVNRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQQ 507
                             EM NRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQQ
Sbjct: 425 ------------------EMANRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQQ 484

Query: 508 ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA 567
           ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA
Sbjct: 485 ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA 544

Query: 568 TQKELEMYGKNELQKSKEIIEELNRKLANYTSIIDSKNVELLNLQTALGQYYAEIEAKEH 627
           TQKELEMYGKNELQKSKEIIEELNRKLANY SIIDSKNVELLNLQTALGQYYAEIEAKEH
Sbjct: 545 TQKELEMYGKNELQKSKEIIEELNRKLANYMSIIDSKNVELLNLQTALGQYYAEIEAKEH 604

Query: 628 LESDLAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWKSRVNKLEED 687
           LESDLAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWKSRVNKLEED
Sbjct: 605 LESDLAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWKSRVNKLEED 664

Query: 688 NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED 747
           NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED
Sbjct: 665 NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED 724

Query: 748 DKMRIGAAKQGPSKGVVRGVLGLPGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK 807
           DKMRIGAAKQGPSKGVVRGVLG PGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK
Sbjct: 725 DKMRIGAAKQGPSKGVVRGVLGFPGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK 784

Query: 808 ENEEREKREAKESLKLQEESQLNGPNVGS-----IDPGTRATGSTSESSRTGFPSHHHHH 867
           ENEEREKREAKESLKLQEESQLNGPNVGS     +DPGT+ATGSTSESSRTGFPSHHH H
Sbjct: 785 ENEEREKREAKESLKLQEESQLNGPNVGSTGSQLLDPGTKATGSTSESSRTGFPSHHHRH 787

Query: 868 HHHQSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY 886
           H  QSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY
Sbjct: 845 H--QSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY 787

BLAST of Cp4.1LG12g03990 vs. NCBI nr
Match: KAG6575201.1 (Golgin candidate 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1319 bits (3414), Expect = 0.0
Identity = 754/879 (85.78%), Postives = 758/879 (86.23%), Query Frame = 0

Query: 28  SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA 87
           SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA
Sbjct: 5   SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA 64

Query: 88  NGIEDAHHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKE---------------SL 147
           NGIEDA HPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKE               SL
Sbjct: 65  NGIEDARHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKEELILRLNKENGSLKQSL 124

Query: 148 EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGIANGASHSG 207
           EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGI NGASHSG
Sbjct: 125 EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGITNGASHSG 184

Query: 208 KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQERDQLADVQLRLR 267
           KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQER+QLADVQLRLR
Sbjct: 185 KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQEREQLADVQLRLR 244

Query: 268 EEQKLNKKFQEELNSLHMNKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNV 327
           EEQKLNKKFQEELNSLHMNKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNV
Sbjct: 245 EEQKLNKKFQEELNSLHMNKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNV 304

Query: 328 EGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSLEMVNRHLSGSNEASLE 387
           EGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSL                
Sbjct: 305 EGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSL---------------- 364

Query: 388 MSNIIRELNEKKLEVKQLQVELNRRENMKSDDNVEGLKRLITKLEKEKSTLEMGKKELED 447
                                                                       
Sbjct: 365 ------------------------------------------------------------ 424

Query: 448 TLEKCRTSSSVEAQSSSLEMVNRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQQ 507
                             EMVNRHLSGSNEKLGLSGIS GKEDMDLS+QKLKKDLKEMQQ
Sbjct: 425 ------------------EMVNRHLSGSNEKLGLSGISSGKEDMDLSMQKLKKDLKEMQQ 484

Query: 508 ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA 567
           ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA
Sbjct: 485 ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA 544

Query: 568 TQKELEMYGKNELQKSKEIIEELNRKLANYTSIIDSKNVELLNLQTALGQYYAEIEAKEH 627
           TQKELEMYGKNELQKSKEIIEELNRKLANY SIIDSKNVELLNLQTALGQYYAEIEAKEH
Sbjct: 545 TQKELEMYGKNELQKSKEIIEELNRKLANYMSIIDSKNVELLNLQTALGQYYAEIEAKEH 604

Query: 628 LESDLAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWKSRVNKLEED 687
           LESDLAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWKSRVNKLEED
Sbjct: 605 LESDLAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWKSRVNKLEED 664

Query: 688 NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED 747
           NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED
Sbjct: 665 NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED 724

Query: 748 DKMRIGAAKQGPSKGVVRGVLGLPGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK 807
           DKMRIGAAKQGPSKGVVRGVLGLPGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK
Sbjct: 725 DKMRIGAAKQGPSKGVVRGVLGLPGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK 784

Query: 808 ENEEREKREAKESLKLQEESQLNGPNVGS-----IDPGTRATGSTSESSRTGFPSHHHHH 867
           ENEEREKREAKESLKLQEESQLNGPNVGS     +DPGT+ATGSTSESSRTGFPSHHH H
Sbjct: 785 ENEEREKREAKESLKLQEESQLNGPNVGSTGSQLLDPGTKATGSTSESSRTGFPSHHHRH 787

Query: 868 HHHQSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY 886
           H  QSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY
Sbjct: 845 H--QSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY 787

BLAST of Cp4.1LG12g03990 vs. NCBI nr
Match: XP_022959209.1 (golgin candidate 3-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1317 bits (3408), Expect = 0.0
Identity = 752/879 (85.55%), Postives = 756/879 (86.01%), Query Frame = 0

Query: 28  SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA 87
           SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA
Sbjct: 5   SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA 64

Query: 88  NGIEDAHHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKE---------------SL 147
           NGIEDA HPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKE               SL
Sbjct: 65  NGIEDARHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKEELILRLNKENGSLKQSL 124

Query: 148 EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGIANGASHSG 207
           EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGI NGASHSG
Sbjct: 125 EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGITNGASHSG 184

Query: 208 KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQERDQLADVQLRLR 267
           KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQER+QLADVQLRLR
Sbjct: 185 KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQEREQLADVQLRLR 244

Query: 268 EEQKLNKKFQEELNSLHMNKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNV 327
           EEQKLNKKFQEELNSLH+NKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNV
Sbjct: 245 EEQKLNKKFQEELNSLHVNKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNV 304

Query: 328 EGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSLEMVNRHLSGSNEASLE 387
           EGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSL                
Sbjct: 305 EGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSL---------------- 364

Query: 388 MSNIIRELNEKKLEVKQLQVELNRRENMKSDDNVEGLKRLITKLEKEKSTLEMGKKELED 447
                                                                       
Sbjct: 365 ------------------------------------------------------------ 424

Query: 448 TLEKCRTSSSVEAQSSSLEMVNRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQQ 507
                             EM NRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQQ
Sbjct: 425 ------------------EMANRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQQ 484

Query: 508 ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA 567
           ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA
Sbjct: 485 ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA 544

Query: 568 TQKELEMYGKNELQKSKEIIEELNRKLANYTSIIDSKNVELLNLQTALGQYYAEIEAKEH 627
           TQKELEMYGKNELQKSKEIIEELNRKLANY SIIDSKNVELLNLQTALGQYYAEIEAKEH
Sbjct: 545 TQKELEMYGKNELQKSKEIIEELNRKLANYMSIIDSKNVELLNLQTALGQYYAEIEAKEH 604

Query: 628 LESDLAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWKSRVNKLEED 687
           LESDLAREREGEAKLSQMLKDANQREDAL KEKEEILSKLSLSERALGEWKSRVNKLEED
Sbjct: 605 LESDLAREREGEAKLSQMLKDANQREDALNKEKEEILSKLSLSERALGEWKSRVNKLEED 664

Query: 688 NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED 747
           NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED
Sbjct: 665 NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED 724

Query: 748 DKMRIGAAKQGPSKGVVRGVLGLPGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK 807
           DKMRIGAAKQGPSKGVVRGVLG PGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK
Sbjct: 725 DKMRIGAAKQGPSKGVVRGVLGFPGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK 784

Query: 808 ENEEREKREAKESLKLQEESQLNGPNVGS-----IDPGTRATGSTSESSRTGFPSHHHHH 867
           ENEEREKREAKESLKLQE SQLNGPNVGS     +DPGT+ATGSTSESSRTGFPSHHHHH
Sbjct: 785 ENEEREKREAKESLKLQEGSQLNGPNVGSTGSQLLDPGTKATGSTSESSRTGFPSHHHHH 787

Query: 868 HHHQSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY 886
           H  QSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY
Sbjct: 845 H--QSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY 787

BLAST of Cp4.1LG12g03990 vs. NCBI nr
Match: XP_022959208.1 (golgin candidate 3-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1317 bits (3408), Expect = 0.0
Identity = 752/879 (85.55%), Postives = 757/879 (86.12%), Query Frame = 0

Query: 28  SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA 87
           SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA
Sbjct: 5   SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA 64

Query: 88  NGIEDAHHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKE---------------SL 147
           NGIEDA HPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKE               SL
Sbjct: 65  NGIEDARHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKEELILRLNKENGSLKQSL 124

Query: 148 EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGIANGASHSG 207
           EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGI NGASHSG
Sbjct: 125 EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGITNGASHSG 184

Query: 208 KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQERDQLADVQLRLR 267
           KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQER+QLADVQLRLR
Sbjct: 185 KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQEREQLADVQLRLR 244

Query: 268 EEQKLNKKFQEELNSLHMNKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNV 327
           EEQKLNKKFQEELNSLH+NKDK                                      
Sbjct: 245 EEQKLNKKFQEELNSLHVNKDK-------------------------------------- 304

Query: 328 EGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSLEMVNRHLSGSNEASLE 387
                                                                  +ASLE
Sbjct: 305 -------------------------------------------------------QASLE 364

Query: 388 MSNIIRELNEKKLEVKQLQVELNRRENMKSDDNVEGLKRLITKLEKEKSTLEMGKKELED 447
           MSNIIRELNEKKLEVKQLQVELNRRENMKSDDNVEGLKRLITKLEKEKSTLEMGKKELED
Sbjct: 365 MSNIIRELNEKKLEVKQLQVELNRRENMKSDDNVEGLKRLITKLEKEKSTLEMGKKELED 424

Query: 448 TLEKCRTSSSVEAQSSSLEMVNRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQQ 507
           TLEKCRTSSSVEAQSSSLEM NRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQQ
Sbjct: 425 TLEKCRTSSSVEAQSSSLEMANRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQQ 484

Query: 508 ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA 567
           ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA
Sbjct: 485 ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA 544

Query: 568 TQKELEMYGKNELQKSKEIIEELNRKLANYTSIIDSKNVELLNLQTALGQYYAEIEAKEH 627
           TQKELEMYGKNELQKSKEIIEELNRKLANY SIIDSKNVELLNLQTALGQYYAEIEAKEH
Sbjct: 545 TQKELEMYGKNELQKSKEIIEELNRKLANYMSIIDSKNVELLNLQTALGQYYAEIEAKEH 604

Query: 628 LESDLAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWKSRVNKLEED 687
           LESDLAREREGEAKLSQMLKDANQREDAL KEKEEILSKLSLSERALGEWKSRVNKLEED
Sbjct: 605 LESDLAREREGEAKLSQMLKDANQREDALNKEKEEILSKLSLSERALGEWKSRVNKLEED 664

Query: 688 NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED 747
           NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED
Sbjct: 665 NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED 724

Query: 748 DKMRIGAAKQGPSKGVVRGVLGLPGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK 807
           DKMRIGAAKQGPSKGVVRGVLG PGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK
Sbjct: 725 DKMRIGAAKQGPSKGVVRGVLGFPGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK 784

Query: 808 ENEEREKREAKESLKLQEESQLNGPNVGS-----IDPGTRATGSTSESSRTGFPSHHHHH 867
           ENEEREKREAKESLKLQE SQLNGPNVGS     +DPGT+ATGSTSESSRTGFPSHHHHH
Sbjct: 785 ENEEREKREAKESLKLQEGSQLNGPNVGSTGSQLLDPGTKATGSTSESSRTGFPSHHHHH 788

Query: 868 HHHQSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY 886
           H  QSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY
Sbjct: 845 H--QSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY 788

BLAST of Cp4.1LG12g03990 vs. ExPASy TrEMBL
Match: A0A6J1H5A8 (golgin candidate 3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111460268 PE=4 SV=1)

HSP 1 Score: 1317 bits (3408), Expect = 0.0
Identity = 752/879 (85.55%), Postives = 757/879 (86.12%), Query Frame = 0

Query: 28  SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA 87
           SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA
Sbjct: 5   SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA 64

Query: 88  NGIEDAHHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKE---------------SL 147
           NGIEDA HPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKE               SL
Sbjct: 65  NGIEDARHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKEELILRLNKENGSLKQSL 124

Query: 148 EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGIANGASHSG 207
           EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGI NGASHSG
Sbjct: 125 EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGITNGASHSG 184

Query: 208 KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQERDQLADVQLRLR 267
           KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQER+QLADVQLRLR
Sbjct: 185 KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQEREQLADVQLRLR 244

Query: 268 EEQKLNKKFQEELNSLHMNKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNV 327
           EEQKLNKKFQEELNSLH+NKDK                                      
Sbjct: 245 EEQKLNKKFQEELNSLHVNKDK-------------------------------------- 304

Query: 328 EGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSLEMVNRHLSGSNEASLE 387
                                                                  +ASLE
Sbjct: 305 -------------------------------------------------------QASLE 364

Query: 388 MSNIIRELNEKKLEVKQLQVELNRRENMKSDDNVEGLKRLITKLEKEKSTLEMGKKELED 447
           MSNIIRELNEKKLEVKQLQVELNRRENMKSDDNVEGLKRLITKLEKEKSTLEMGKKELED
Sbjct: 365 MSNIIRELNEKKLEVKQLQVELNRRENMKSDDNVEGLKRLITKLEKEKSTLEMGKKELED 424

Query: 448 TLEKCRTSSSVEAQSSSLEMVNRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQQ 507
           TLEKCRTSSSVEAQSSSLEM NRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQQ
Sbjct: 425 TLEKCRTSSSVEAQSSSLEMANRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQQ 484

Query: 508 ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA 567
           ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA
Sbjct: 485 ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA 544

Query: 568 TQKELEMYGKNELQKSKEIIEELNRKLANYTSIIDSKNVELLNLQTALGQYYAEIEAKEH 627
           TQKELEMYGKNELQKSKEIIEELNRKLANY SIIDSKNVELLNLQTALGQYYAEIEAKEH
Sbjct: 545 TQKELEMYGKNELQKSKEIIEELNRKLANYMSIIDSKNVELLNLQTALGQYYAEIEAKEH 604

Query: 628 LESDLAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWKSRVNKLEED 687
           LESDLAREREGEAKLSQMLKDANQREDAL KEKEEILSKLSLSERALGEWKSRVNKLEED
Sbjct: 605 LESDLAREREGEAKLSQMLKDANQREDALNKEKEEILSKLSLSERALGEWKSRVNKLEED 664

Query: 688 NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED 747
           NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED
Sbjct: 665 NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED 724

Query: 748 DKMRIGAAKQGPSKGVVRGVLGLPGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK 807
           DKMRIGAAKQGPSKGVVRGVLG PGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK
Sbjct: 725 DKMRIGAAKQGPSKGVVRGVLGFPGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK 784

Query: 808 ENEEREKREAKESLKLQEESQLNGPNVGS-----IDPGTRATGSTSESSRTGFPSHHHHH 867
           ENEEREKREAKESLKLQE SQLNGPNVGS     +DPGT+ATGSTSESSRTGFPSHHHHH
Sbjct: 785 ENEEREKREAKESLKLQEGSQLNGPNVGSTGSQLLDPGTKATGSTSESSRTGFPSHHHHH 788

Query: 868 HHHQSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY 886
           H  QSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY
Sbjct: 845 H--QSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY 788

BLAST of Cp4.1LG12g03990 vs. ExPASy TrEMBL
Match: A0A6J1H7B7 (golgin candidate 3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111460268 PE=4 SV=1)

HSP 1 Score: 1317 bits (3408), Expect = 0.0
Identity = 752/879 (85.55%), Postives = 756/879 (86.01%), Query Frame = 0

Query: 28  SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA 87
           SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA
Sbjct: 5   SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA 64

Query: 88  NGIEDAHHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKE---------------SL 147
           NGIEDA HPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKE               SL
Sbjct: 65  NGIEDARHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKEELILRLNKENGSLKQSL 124

Query: 148 EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGIANGASHSG 207
           EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGI NGASHSG
Sbjct: 125 EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGITNGASHSG 184

Query: 208 KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQERDQLADVQLRLR 267
           KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQER+QLADVQLRLR
Sbjct: 185 KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQEREQLADVQLRLR 244

Query: 268 EEQKLNKKFQEELNSLHMNKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNV 327
           EEQKLNKKFQEELNSLH+NKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNV
Sbjct: 245 EEQKLNKKFQEELNSLHVNKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNV 304

Query: 328 EGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSLEMVNRHLSGSNEASLE 387
           EGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSL                
Sbjct: 305 EGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSL---------------- 364

Query: 388 MSNIIRELNEKKLEVKQLQVELNRRENMKSDDNVEGLKRLITKLEKEKSTLEMGKKELED 447
                                                                       
Sbjct: 365 ------------------------------------------------------------ 424

Query: 448 TLEKCRTSSSVEAQSSSLEMVNRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQQ 507
                             EM NRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQQ
Sbjct: 425 ------------------EMANRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQQ 484

Query: 508 ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA 567
           ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA
Sbjct: 485 ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA 544

Query: 568 TQKELEMYGKNELQKSKEIIEELNRKLANYTSIIDSKNVELLNLQTALGQYYAEIEAKEH 627
           TQKELEMYGKNELQKSKEIIEELNRKLANY SIIDSKNVELLNLQTALGQYYAEIEAKEH
Sbjct: 545 TQKELEMYGKNELQKSKEIIEELNRKLANYMSIIDSKNVELLNLQTALGQYYAEIEAKEH 604

Query: 628 LESDLAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWKSRVNKLEED 687
           LESDLAREREGEAKLSQMLKDANQREDAL KEKEEILSKLSLSERALGEWKSRVNKLEED
Sbjct: 605 LESDLAREREGEAKLSQMLKDANQREDALNKEKEEILSKLSLSERALGEWKSRVNKLEED 664

Query: 688 NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED 747
           NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED
Sbjct: 665 NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED 724

Query: 748 DKMRIGAAKQGPSKGVVRGVLGLPGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK 807
           DKMRIGAAKQGPSKGVVRGVLG PGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK
Sbjct: 725 DKMRIGAAKQGPSKGVVRGVLGFPGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK 784

Query: 808 ENEEREKREAKESLKLQEESQLNGPNVGS-----IDPGTRATGSTSESSRTGFPSHHHHH 867
           ENEEREKREAKESLKLQE SQLNGPNVGS     +DPGT+ATGSTSESSRTGFPSHHHHH
Sbjct: 785 ENEEREKREAKESLKLQEGSQLNGPNVGSTGSQLLDPGTKATGSTSESSRTGFPSHHHHH 787

Query: 868 HHHQSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY 886
           H  QSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY
Sbjct: 845 H--QSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY 787

BLAST of Cp4.1LG12g03990 vs. ExPASy TrEMBL
Match: A0A6J1KW22 (golgin candidate 4-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499218 PE=4 SV=1)

HSP 1 Score: 1309 bits (3387), Expect = 0.0
Identity = 748/879 (85.10%), Postives = 754/879 (85.78%), Query Frame = 0

Query: 28  SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA 87
           SIANLKENLNKIALDVHHDDDEEEF+IYGSN GDVDVSVSDRRNSHSFAHSNPVTRSPI 
Sbjct: 5   SIANLKENLNKIALDVHHDDDEEEFSIYGSNRGDVDVSVSDRRNSHSFAHSNPVTRSPIV 64

Query: 88  NGIEDAHHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKE---------------SL 147
           NGIEDA HPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKE               SL
Sbjct: 65  NGIEDARHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKEELILRLNKENGSLKQSL 124

Query: 148 EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGIANGASHSG 207
           EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGI NGASHSG
Sbjct: 125 EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGITNGASHSG 184

Query: 208 KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQERDQLADVQLRLR 267
           KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQER+QLADVQLRLR
Sbjct: 185 KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQEREQLADVQLRLR 244

Query: 268 EEQKLNKKFQEELNSLHMNKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNV 327
           EEQKLNKKFQEELNSLHMNKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNV
Sbjct: 245 EEQKLNKKFQEELNSLHMNKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNV 304

Query: 328 EGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSLEMVNRHLSGSNEASLE 387
           EGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSL                
Sbjct: 305 EGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSL---------------- 364

Query: 388 MSNIIRELNEKKLEVKQLQVELNRRENMKSDDNVEGLKRLITKLEKEKSTLEMGKKELED 447
                                                                       
Sbjct: 365 ------------------------------------------------------------ 424

Query: 448 TLEKCRTSSSVEAQSSSLEMVNRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQQ 507
                             EMVNRHLSGSNEKLGLS ISPGKEDMDLS+QKLKKDLKEMQQ
Sbjct: 425 ------------------EMVNRHLSGSNEKLGLSAISPGKEDMDLSMQKLKKDLKEMQQ 484

Query: 508 ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA 567
           ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA
Sbjct: 485 ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA 544

Query: 568 TQKELEMYGKNELQKSKEIIEELNRKLANYTSIIDSKNVELLNLQTALGQYYAEIEAKEH 627
           TQKELEMYGKNELQKSKEIIEELNRKLANY SIIDSKNVELLNLQTALGQYYAEIEAKEH
Sbjct: 545 TQKELEMYGKNELQKSKEIIEELNRKLANYMSIIDSKNVELLNLQTALGQYYAEIEAKEH 604

Query: 628 LESDLAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWKSRVNKLEED 687
           LESDLAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWKSRVNKLEED
Sbjct: 605 LESDLAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWKSRVNKLEED 664

Query: 688 NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED 747
           NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED
Sbjct: 665 NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED 724

Query: 748 DKMRIGAAKQGPSKGVVRGVLGLPGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK 807
           DKMRIGAAKQGPSKGVVRGVLG PGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK
Sbjct: 725 DKMRIGAAKQGPSKGVVRGVLGFPGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK 784

Query: 808 ENEEREKREAKESLKLQEESQLNGPNVGS-----IDPGTRATGSTSESSRTGFPSHHHHH 867
           ENEEREKREAKESLKLQEESQLNGPNVG+     +DP T+ATGSTSESSRTGFPSHHHHH
Sbjct: 785 ENEEREKREAKESLKLQEESQLNGPNVGNTGSQLLDPRTKATGSTSESSRTGFPSHHHHH 786

Query: 868 HHHQSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY 886
              QSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY
Sbjct: 845 ---QSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY 786

BLAST of Cp4.1LG12g03990 vs. ExPASy TrEMBL
Match: A0A6J1KXY9 (golgin candidate 4-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499218 PE=4 SV=1)

HSP 1 Score: 1309 bits (3387), Expect = 0.0
Identity = 748/879 (85.10%), Postives = 755/879 (85.89%), Query Frame = 0

Query: 28  SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA 87
           SIANLKENLNKIALDVHHDDDEEEF+IYGSN GDVDVSVSDRRNSHSFAHSNPVTRSPI 
Sbjct: 5   SIANLKENLNKIALDVHHDDDEEEFSIYGSNRGDVDVSVSDRRNSHSFAHSNPVTRSPIV 64

Query: 88  NGIEDAHHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKE---------------SL 147
           NGIEDA HPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKE               SL
Sbjct: 65  NGIEDARHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKEELILRLNKENGSLKQSL 124

Query: 148 EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGIANGASHSG 207
           EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGI NGASHSG
Sbjct: 125 EGTNTSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGITNGASHSG 184

Query: 208 KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQERDQLADVQLRLR 267
           KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQER+QLADVQLRLR
Sbjct: 185 KLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQEREQLADVQLRLR 244

Query: 268 EEQKLNKKFQEELNSLHMNKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNV 327
           EEQKLNKKFQEELNSLHMNKDK                                      
Sbjct: 245 EEQKLNKKFQEELNSLHMNKDK-------------------------------------- 304

Query: 328 EGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSLEMVNRHLSGSNEASLE 387
                                                                  +ASLE
Sbjct: 305 -------------------------------------------------------QASLE 364

Query: 388 MSNIIRELNEKKLEVKQLQVELNRRENMKSDDNVEGLKRLITKLEKEKSTLEMGKKELED 447
           MSNIIRELNEKKLEVKQLQVELNRRENMKSDDNVEGLKRLITKLEKEKSTLEMGKKELED
Sbjct: 365 MSNIIRELNEKKLEVKQLQVELNRRENMKSDDNVEGLKRLITKLEKEKSTLEMGKKELED 424

Query: 448 TLEKCRTSSSVEAQSSSLEMVNRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQQ 507
           TLEKCRTSSSVEAQSSSLEMVNRHLSGSNEKLGLS ISPGKEDMDLS+QKLKKDLKEMQQ
Sbjct: 425 TLEKCRTSSSVEAQSSSLEMVNRHLSGSNEKLGLSAISPGKEDMDLSMQKLKKDLKEMQQ 484

Query: 508 ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA 567
           ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA
Sbjct: 485 ERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIA 544

Query: 568 TQKELEMYGKNELQKSKEIIEELNRKLANYTSIIDSKNVELLNLQTALGQYYAEIEAKEH 627
           TQKELEMYGKNELQKSKEIIEELNRKLANY SIIDSKNVELLNLQTALGQYYAEIEAKEH
Sbjct: 545 TQKELEMYGKNELQKSKEIIEELNRKLANYMSIIDSKNVELLNLQTALGQYYAEIEAKEH 604

Query: 628 LESDLAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWKSRVNKLEED 687
           LESDLAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWKSRVNKLEED
Sbjct: 605 LESDLAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWKSRVNKLEED 664

Query: 688 NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED 747
           NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED
Sbjct: 665 NSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSED 724

Query: 748 DKMRIGAAKQGPSKGVVRGVLGLPGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK 807
           DKMRIGAAKQGPSKGVVRGVLG PGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK
Sbjct: 725 DKMRIGAAKQGPSKGVVRGVLGFPGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLK 784

Query: 808 ENEEREKREAKESLKLQEESQLNGPNVGS-----IDPGTRATGSTSESSRTGFPSHHHHH 867
           ENEEREKREAKESLKLQEESQLNGPNVG+     +DP T+ATGSTSESSRTGFPSHHHHH
Sbjct: 785 ENEEREKREAKESLKLQEESQLNGPNVGNTGSQLLDPRTKATGSTSESSRTGFPSHHHHH 787

Query: 868 HHHQSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY 886
              QSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY
Sbjct: 845 ---QSTHLPFGGDFRLSRHHSESEFSTVPLTSTTENTHY 787

BLAST of Cp4.1LG12g03990 vs. ExPASy TrEMBL
Match: A0A5D3CUW8 (Golgin candidate 4 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold268G00260 PE=4 SV=1)

HSP 1 Score: 1146 bits (2965), Expect = 0.0
Identity = 672/874 (76.89%), Postives = 709/874 (81.12%), Query Frame = 0

Query: 28  SIANLKENLNKIALDVHHDDDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSPIA 87
           SIANLKENLNKIALDVH+DDDEEEFAIYGSN GD DVSVSDRRNSH FAHSNPVTRSP+A
Sbjct: 5   SIANLKENLNKIALDVHNDDDEEEFAIYGSNRGDADVSVSDRRNSHRFAHSNPVTRSPVA 64

Query: 88  NGIEDAHHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKE-----------SLEGTN 147
           NGIEDA HPEIEQYK EIKRLQESER+IKSLSMNYAALLKEKE           SL+ + 
Sbjct: 65  NGIEDARHPEIEQYKAEIKRLQESERNIKSLSMNYAALLKEKEELILRLNKENGSLKQSL 124

Query: 148 TSTNSPRAESSKSPSNGTNEMKGSDQSPTRLLRGKTRRNGIVSKQDGIANGASHSGKLDY 207
            +TNSP++ESSKSP+NGT+EMKGSDQSP RLLRGKTRRNG+VSKQDGIANGASHSGK D 
Sbjct: 125 DATNSPKSESSKSPANGTSEMKGSDQSPGRLLRGKTRRNGMVSKQDGIANGASHSGKHDS 184

Query: 208 QSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQERDQLADVQLRLREEQK 267
            SKMVPEHSTSQEL D QEGN+GSLQDVQ TLE+KQLRKELQQER+QLADVQLRLREEQK
Sbjct: 185 LSKMVPEHSTSQELADLQEGNMGSLQDVQATLELKQLRKELQQEREQLADVQLRLREEQK 244

Query: 268 LNKKFQEELNSLHMNKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNVEGLK 327
           LNKKFQEELNSL MNKDKASLEMS+I+RELNEKKLEVKQLQVELNRRE MKSDDNVE LK
Sbjct: 245 LNKKFQEELNSLQMNKDKASLEMSDILRELNEKKLEVKQLQVELNRREKMKSDDNVEELK 304

Query: 328 RLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSLEMVNRHLSGSNEASLEMSNI 387
           RLIT LEKEKSTLEM KKEL+DTLEK R SS V   S SL                    
Sbjct: 305 RLITTLEKEKSTLEMEKKELKDTLEKSRESSGVGTPSKSL-------------------- 364

Query: 388 IRELNEKKLEVKQLQVELNRRENMKSDDNVEGLKRLITKLEKEKSTLEMGKKELEDTLEK 447
                                                                       
Sbjct: 365 ------------------------------------------------------------ 424

Query: 448 CRTSSSVEAQSSSLEMVNRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQQERDK 507
                         EMVNRHLSGS+EKLG SG    KED DLSLQKLKKDLKEMQQERDK
Sbjct: 425 --------------EMVNRHLSGSSEKLGPSG----KEDRDLSLQKLKKDLKEMQQERDK 484

Query: 508 AVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAIATQKE 567
           AVHELSRLKQHLLEKESEESEKMDEDSRIIEELRH+NEYQRGQILHLEKALNQAIATQKE
Sbjct: 485 AVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHNNEYQRGQILHLEKALNQAIATQKE 544

Query: 568 LEMYGKNELQKSKEIIEELNRKLANYTSIIDSKNVELLNLQTALGQYYAEIEAKEHLESD 627
            EMYG NELQKSKEIIE+LNRKLAN  S IDSKN+ELLNLQTALGQYYAEIEAKEHLES 
Sbjct: 545 AEMYGNNELQKSKEIIEDLNRKLANCMSTIDSKNIELLNLQTALGQYYAEIEAKEHLESV 604

Query: 628 LAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWKSRVNKLEEDNSKL 687
           LARERE EAKLSQMLKDANQREDALKKEKEEILSKLS+SERALGEWKSRVNKLEEDNSKL
Sbjct: 605 LAREREEEAKLSQMLKDANQREDALKKEKEEILSKLSISERALGEWKSRVNKLEEDNSKL 664

Query: 688 RRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSEDDKMR 747
           RRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQ+NHSKEVLDLMVRMLGFSED+K+R
Sbjct: 665 RRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQRNHSKEVLDLMVRMLGFSEDEKLR 724

Query: 748 IGAAKQGPSKGVVRGVLGLPGRLVGGILGGSSAETPANMASDNQSFADLWVDFLLKENEE 807
           IGAAKQGPSKGVVRGVLGLPGRLVGGILGGS+AETPANMASDNQSFADLWVDFLLKENEE
Sbjct: 725 IGAAKQGPSKGVVRGVLGLPGRLVGGILGGSAAETPANMASDNQSFADLWVDFLLKENEE 774

Query: 808 REKREAKESLKLQEESQLNGPNVG-----SIDPGTRATGSTSESSRTGFPSHHHHHHHHQ 867
           REKR+A+ESLKL+EESQ +GP+V      S+DP T+ TGST  SSRT FPSH       Q
Sbjct: 785 REKRQAEESLKLREESQSSGPDVALTGSPSLDPRTKTTGSTPNSSRTAFPSHL------Q 774

Query: 868 STHLPFGGDFRLSRHHSESEFSTVPLTSTTENTH 885
           STHLPFG DFRLSRHHS+SEFSTVPLTS++ENT+
Sbjct: 845 STHLPFGNDFRLSRHHSDSEFSTVPLTSSSENTY 774

BLAST of Cp4.1LG12g03990 vs. TAIR 10
Match: AT2G46180.1 (golgin candidate 4 )

HSP 1 Score: 555.4 bits (1430), Expect = 9.1e-158
Identity = 384/811 (47.35%), Postives = 500/811 (61.65%), Query Frame = 0

Query: 27  NSIANLKENLNKIALDVHHD--DDEEEFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRS 86
           +S+ANLKENLNKIA DVH D  DD+E+  IYGS  G      +DRRNS+ F +S    RS
Sbjct: 3   SSVANLKENLNKIAHDVHDDDEDDDEDLTIYGSTNGG-----TDRRNSNGFRYS----RS 62

Query: 87  PIANGIEDAHHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKE-----------SLE 146
           P+ANG E   +PEIE+YK EI +LQ+SE +IK+LS+NYAALLKEKE           SL+
Sbjct: 63  PMANGFESPVNPEIERYKAEINKLQKSESEIKALSVNYAALLKEKEDQISRLNQENGSLK 122

Query: 147 GTNTSTNSPRAES--SKSPSNGTNEMKGS-DQSPTRLLRGKTR-------RNGIVSKQDG 206
              TSTN+   ES    S ++  N +KG+ D SP R  R  T         NGI SK +G
Sbjct: 123 QNLTSTNAALKESRLDLSRASNNNAIKGNGDHSPNRSQRSPTNWKNRNQMNNGIASKPNG 182

Query: 207 IANGASHSGKLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQERDQ 266
             N +               H   +E  +  E    S+   Q         +EL++ER++
Sbjct: 183 TENDSE-------------SHKKEKEFAEMLEERTRSMASAQA--------RELEKEREK 242

Query: 267 LADVQLRLREEQKLNKKFQEELNSLHMNKDKASLEMSNIIRELNEKKLEVKQLQVELNRR 326
            A++Q+ L+EE+K N+ F+EEL SL ++K+K  +E + + REL+ K  E++QLQ++LN  
Sbjct: 243 SANLQILLQEERKQNETFKEELQSLRLDKEKTLMESNKVRRELDAKLAEIRQLQMKLNGG 302

Query: 327 ENMKSDDNVEGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSLEMVNRHL 386
           E      + E LK +   LEKE + L++ + ELE  LE  + S+S +    S E ++RHL
Sbjct: 303 EQHAFGISRENLKEVNKALEKENNELKLKRSELEAALEASQKSTSRKLFPKSTEDLSRHL 362

Query: 387 SGSNEASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNVEGLKRLITKLEKEKSTL 446
           S                                                   L++EK   
Sbjct: 363 S--------------------------------------------------SLDEEK--- 422

Query: 447 EMGKKELEDTLEKCRTSSSVEAQSSSLEMVNRHLSGSNEKLGLSGISPGKEDMDLSLQKL 506
                                                      +G  PGKEDM+ SLQ+L
Sbjct: 423 -------------------------------------------AGTFPGKEDMEKSLQRL 482

Query: 507 KKDLKEMQQERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHL 566
           +K+L+E ++E+DKA  EL RLKQHLLEKE+EESEKMDEDSR+I+ELR  NEYQR QIL L
Sbjct: 483 EKELEEARREKDKARQELKRLKQHLLEKETEESEKMDEDSRLIDELRQTNEYQRSQILGL 542

Query: 567 EKALNQAIATQKELEMYGKNELQKSKEIIEELNRKLANYTSIIDSKNVELLNLQTALGQY 626
           EKAL Q +A Q+E++     E++KSK IIE+LN+KLAN    IDSKNVELLNLQTALGQY
Sbjct: 543 EKALRQTMANQEEIKSSSDLEIRKSKGIIEDLNQKLANCLRTIDSKNVELLNLQTALGQY 602

Query: 627 YAEIEAKEHLESDLAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWK 686
           YAEIEAKEH E +LA  +E   KLS  LKD +++ ++ KKEKEEI SK+  +E    EWK
Sbjct: 603 YAEIEAKEHFERELAVAKEDAMKLSARLKDVDEQLESSKKEKEEITSKVLHAENIAAEWK 662

Query: 687 SRVNKLEEDNSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLM 746
           +RV+K+E+DN+K+RR L+QSMTRLNRMS+DSDFLVDRRIVIKLLVTYFQ+NHS+EVLDLM
Sbjct: 663 NRVSKVEDDNAKVRRVLEQSMTRLNRMSMDSDFLVDRRIVIKLLVTYFQRNHSREVLDLM 687

Query: 747 VRMLGFSEDDKMRIGAAKQGPS-KGVVRGVLGLPGRLVGGIL--GGSSAETPANMASDNQ 806
           VRMLGFSE++K RIG A+QG + KGVVRGVLG PGRLVGGIL  GG S ++  NMASDNQ
Sbjct: 723 VRMLGFSEEEKQRIGLAQQGAAGKGVVRGVLGFPGRLVGGILGGGGGSPDSHPNMASDNQ 687

Query: 807 SFADLWVDFLLKENEEREKREAKESLKLQEE 812
           SFAD+WV+FLLK+ EERE+REA+++   ++E
Sbjct: 783 SFADMWVEFLLKDAEERERREAEDAANKEQE 687

BLAST of Cp4.1LG12g03990 vs. TAIR 10
Match: AT3G61570.1 (GRIP-related ARF-binding domain-containing protein 1 )

HSP 1 Score: 552.4 bits (1422), Expect = 7.7e-157
Identity = 381/804 (47.39%), Postives = 495/804 (61.57%), Query Frame = 0

Query: 27  NSIANLKENLNKIALDVHHDDDEE-EFAIYGSNGGDVDVSVSDRRNSHSFAHSNPVTRSP 86
           +SI N+K NL+KI LDVH DD+EE +   YGS  G   VS SDRRNS  F     V+R  
Sbjct: 3   SSIENMKANLHKIVLDVHEDDEEEDDLHKYGSANG---VSNSDRRNSSGF---RSVSRYS 62

Query: 87  IANGIEDAHHPEIEQYKTEIKRLQESERDIKSLSMNYAALLKEKE-----------SLEG 146
           I+NGIE   H EIE+YK EIK+LQESE DIK+LS+NYAALL+EKE           SL+ 
Sbjct: 63  ISNGIESPAHHEIERYKAEIKKLQESESDIKALSVNYAALLREKEDQISRLNQENGSLKQ 122

Query: 147 TNTSTNSPRAESSKSPSNGTNE--MKG-SDQSPTRLLRGKTRRNGIVSKQDGIANGASHS 206
             TST++   E+    S G+N   +KG +DQSP RL +  +     +   + ++NG    
Sbjct: 123 NLTSTSAALKEARTDISRGSNNYAIKGNNDQSPNRLHKSVSH----LKSPNHMSNG---- 182

Query: 207 GKLDYQSKMVPEHSTSQELTDFQEGNIGSLQDVQTTLEMKQLRKELQQERDQLADVQLRL 266
                + K        ++L D  E    S+  VQ T        EL +ER++L D QL L
Sbjct: 183 -----KGKDTDSFIKEKDLADMLEDRTKSMAAVQAT--------ELAKEREKLRDFQLSL 242

Query: 267 REEQKLNKKFQEELNSLHMNKDKASLEMSNIIRELNEKKLEVKQLQVELNRRENMKSDDN 326
           +EE+K ++ F+EEL S+ ++K+K S+E+S +  EL+ K LE+K LQ++L  +E+      
Sbjct: 243 QEERKRSESFKEELESMRLDKNKTSMEISKMRSELDAKLLEIKHLQMKLTGQESHAIGPG 302

Query: 327 VEGLKRLITKLEKEKSTLEMGKKELEDTLEKCRTSSSVEAQSSSLEMVNRHLSGSNEASL 386
           +E LK +   LEKE + L++ + ELE  LE+ R  ++ +    + E + RH         
Sbjct: 303 MEHLKEVNKALEKENNELKLKRSELEAALEESRKLTNSKVFPDATESLTRH--------- 362

Query: 387 EMSNIIRELNEKKLEVKQLQVELNRRENMKSDDNVEGLKRLITKLEKEKSTLEMGKKELE 446
                                                            STL+  K E  
Sbjct: 363 ------------------------------------------------PSTLDKEKPE-- 422

Query: 447 DTLEKCRTSSSVEAQSSSLEMVNRHLSGSNEKLGLSGISPGKEDMDLSLQKLKKDLKEMQ 506
                                                  PGKE+M+ SLQ+L+ DLKE Q
Sbjct: 423 -------------------------------------SFPGKEEMEQSLQRLEMDLKETQ 482

Query: 507 QERDKAVHELSRLKQHLLEKESEESEKMDEDSRIIEELRHDNEYQRGQILHLEKALNQAI 566
           +ERDKA  EL RLKQHLLEKE+EESEKMDEDSR+IEELR  NEYQR QI HLEK+L QAI
Sbjct: 483 RERDKARQELKRLKQHLLEKETEESEKMDEDSRLIEELRQTNEYQRSQISHLEKSLKQAI 542

Query: 567 ATQKELEMYGKNELQKSKEIIEELNRKLANYTSIIDSKNVELLNLQTALGQYYAEIEAKE 626
           + Q++  +   N+++K K+ +++LN+KL N    I+SKNVELLNLQTALGQYYAEIEAKE
Sbjct: 543 SNQEDNRLSNDNQIRKLKDTVDDLNQKLTNCLRTIESKNVELLNLQTALGQYYAEIEAKE 602

Query: 627 HLESDLAREREGEAKLSQMLKDANQREDALKKEKEEILSKLSLSERALGEWKSRVNKLEE 686
           H E +LA  ++   KLS  LKD+++R ++  KEKE++ SKL  +E+   EWK+RV K+EE
Sbjct: 603 HFERELAMAKDELMKLSARLKDSDERLESSNKEKEDVTSKLLHAEKVAAEWKNRVTKVEE 662

Query: 687 DNSKLRRALDQSMTRLNRMSVDSDFLVDRRIVIKLLVTYFQKNHSKEVLDLMVRMLGFSE 746
           DN+K+RR L+QSMTRLNRMS++SD+LVDRRIVIKLLVTYFQKNH+KEVLDLMVRMLGFSE
Sbjct: 663 DNAKVRRVLEQSMTRLNRMSMESDYLVDRRIVIKLLVTYFQKNHNKEVLDLMVRMLGFSE 683

Query: 747 DDKMRIGAAKQGPSKGVVRGVLGLPGRLVGGILGGSSAETPANMASDNQSFADLWVDFLL 806
           +DK RIGAAKQG  KGVVRGVLG PGR VGGILGG SAE  AN ASDNQSFADLWVDFLL
Sbjct: 723 EDKERIGAAKQGGGKGVVRGVLGFPGRFVGGILGGKSAELHANAASDNQSFADLWVDFLL 683

Query: 807 KENEEREKREAKESL--KLQEESQ 814
           K+ EERE+REA+E+   K +++S+
Sbjct: 783 KDAEERERREAEEAAASKAKQDSE 683