Cp4.1LG12g03160 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG12g03160
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	enoyl-CoA hydratase/isomerase A
Location	Cp4.1LG12: 2150194 .. 2153529 (+)
RNA-Seq Expression	Cp4.1LG12g03160
Synteny	Cp4.1LG12g03160

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CATTATTTGTACCAATGAATTCAATTGTAACTCATTTTTCATTAATGGCTACGAACTTATGAAACTAATAATTTTTCTAATCTTCAAATATTTGATCGATTTTTACCTGACTGGATTTATGTGGAGAATTTCCCGAACAGATAAGTCATATGGGTGAAATTTATCCTTTGTTCATGATTTGCTCGTTTTCCAATACGCAATCTTGCAAGAACTACCCCATGCCTACTTTTTTATTCAATCTCCACTCCCCAATTTGATCACGCCGACTTCCACTTTCTGCTGTATTCGCTCCTGATTTCTTGGCCGGAATGGATCGATCATCGCCGGAAAAGCTCATCCTCGTCAACCGGGAACCAAACGGGATCGCATTCGTTACGATCAACCGACCGAAGTCGTTGAATTCGTTAACGAGAGCGATGATGGCAGATTTGGCTCAGGCGTTTAAGAGCTTGGACCGCGACGAGTCCGTGCGAGTTATCGTGCTCTCTGGATCAGGCCGAGCCTTCTGTTCCGGTGTAGACCTTACTGCGGCGGAGGATGTTTTCAAAGGAGATGTGAAGGATGTTGAAGCCGATCCGGTTGTGCAGATGGAACGCTGCCGGAAGCCGATAATCGGAGCGGTTGCAGGTTTCGCCATCACCGCTGGATTTGAGATCGCCTTGGCTTGCGACATACTCATAGCTGCCAAAGGAGCTAAGTTCATCGATACTCATGCTAGGTTTGTTATTTTCCGACTCAATTTTTTTGTAATCTCGAGTGATTCTTGCTGCTAGGAATAGGATAAACACGGATTTAGGATCAATAAACCAAGTAATTTTCTGAAATCTCCTGCCAATTCTGTTCTGTTGAAACTCCGAATTTCTGGAAAATTTCGTGATAGGTTTGGGATATTTCCTTCGTGGGGATTATCTCAGAAACTCCCGAGGATCATCGGAGTTAGCAAAGCTCGTGAAGTATCCTTAACGGCGATGCCTCTAACTGCAGAGGAAGCCGAGAGGAAGGGTTTGGTGAATCACGTGGTCGACGGAGGTGAATTGTTGAAGAAAACTCGAGAGATTGCGGAGGCCATCTTGAAGAACAATCAGGATTTGGTGTTGAGATACAAGTCCGTCATTAACGATGGCCTGAAGCTGGATCTTGGCCATGCTCTTGCGCTGGAGAAGGTAATTTCCTTGACAAAATTCATATTCCAAATCTGTTGATCATTGAACCTTGAAAATTGTGTTCTTATTTCTGTTAAACTCGAGTTTCTCTTCGAATTTTTGTGCCAATAACTGTCTGGGGATGATTTTTTAAGTACTTTTTGGATGAACAAAGCTTGTGTAGTAGCATCCTGCAACTAGGGATGTCCAATGTTCATTTACACCATGGGGAATTGCTCCGAATGGGGTTTTCCTCTTTACACAGGAAATGGGGAAGGAGGAGCAGGGAGAATTTTCAAAGAATATTCATCGTCCCCGCTCTTGTTCCCGTCCTTGCCTCGAATGGAGTTTCCTCCCTACACAGGAAGTTGGGAAGGAGGAGCGGGGGGAATTTTCGAAGAATATTCGTTGTCCCCGCTCTCGTTCCCGTCCTCGCCCCGAATGGGGTTTCCTCTTTACACAGGAAGTGGGGAAGGAGGAGGAAGTTGGTTTTTAGTGTTCGGTGGTGTTCGACATAGGGGAGAACATCCAAGCCGTGGAATTCACACGAAAGGGAACAAGGAATGATCAACGAAAAAAGTGCTCTCTAAACGTGAAAGTTTTCCTACTGGGAAAGTCTTCCTCGACACGAAGTTCCTTACCCTTCCAGTTTCACTTTCTTCCCTTGTTTCCGCTACTCTTGTTCCCGTCCTTGCCCGTTTCTTTTATCTAAAAAAGTGCTCTCTAAACGTGAAAGTTTTCCTACTGGGAAAGTCTTCCTCGACACGAAGTTCCTTACCCTTCCAGTTTCACTTTCTTCCCTTGTTTCCGCTACTCTTGTTCCCGTCCTTGCCCGTTTCTTTTATCTAAATGGAGAATAATTCTTGACGAGGAATCCTCATCCCAATCCAAAACTTTGCGGGGATGGAAACGAAGCATTTCTACCTATACAACTGACTGTTTGTTTTGTTTGTTTGTTTTGTTGATGCAGGAGAGAGGTCATGCATATTACAATGGAATGACCAAAGAGCAATTCAAGAAAATGCAGGAATTCATAGCTGGTCGGAGCTCCAAGAAGCCTTCCAAGTTATAAGTCTAAACGATGTAGCTTACTGTCTGTTTCTACACTGCAACAATAATAAGAAGAACTTGCCTCCTAAACGGTCAGTGGATTCTTCCCTACTTTTATTCACTTGTAATTTGATTTTAGTGGATCAGAGATAAAAATGACTGTTTTGAGAAGAATTCAGGCTGGTTTCTGAGCAATAAATGTTGGGTATTTTATGGATAATGGCTGAATATGAACTCATTGGATGGCAGTTTATGGTTGATTTGATATCACATTGAAAGGGTAAATTACAAGTAACAATTATTTGTGAAGGAAAAGTCCATAAGACTTTCAAAAGAAGCAAGACACGAAGCGGTACGCCAACGAGGATGTTCGGCTTCTTCTAAGGGTGGATGTTGAGATCCCACGTCGATTGGCCAGACACTTGGCGGTGTGCCAACGAGGACGTTGGACCCCTAAGAGGGGTGGATGGATTGTGAGATCCCACATCGGTCGGCCAGACACCGAGCGCTAAGCCAACAAGGACGTTGGGCCCCCAAAATGGGTGGAATTGTGAGATCTCATGTGTTGGTTGGAGAGGAGAACAAAACATTTCTTATAAGGGTGTGAAAATCTCTCTCTAACCTTTAATACCTTTAGCGGAAAGTTCAAAAAGGACAATACCGACTATTGGTGGGCTTGGGTTATTACATGAAAGCATTAACCCGCTGATATGTCTTTGAGTTTAGTCAGCAAATAACCCGAACCCGTTGAGTAGCAATGAAATTAAACCACAATTGAAAGAGAATGACTCGCCATACTGTATAGAAGTTTCTACTAGATTAGGCAAAAAAAAAAAAAACCTTCTACGTGGACCACTAGGCCTTGCAAAAGCAGGGGCATTGTTGGTCGTGCATGTAAGACAACGTTGTTGGGTATGTGAAGACGTGTCTCCAACAAGACAAGGTCACAGACTCTTATTGGGCCCAAATACTATTCTTTTAGCCCAAATAAAATGATTAGATGGGCCAAGAGGCCCAAGTCACAACCATAAAACTGACCCATACATGATAATGTTTGGAAAAATAGCACACATTTTGTGTATGGAGTAAAACAAATTATTATTTTCTGACCCATTATTTTCAGCTTTAATTGATAATAATTCTT

mRNA sequence

CATTATTTGTACCAATGAATTCAATTGTAACTCATTTTTCATTAATGGCTACGAACTTATGAAACTAATAATTTTTCTAATCTTCAAATATTTGATCGATTTTTACCTGACTGGATTTATGTGGAGAATTTCCCGAACAGATAAGTCATATGGGTGAAATTTATCCTTTGTTCATGATTTGCTCGTTTTCCAATACGCAATCTTGCAAGAACTACCCCATGCCTACTTTTTTATTCAATCTCCACTCCCCAATTTGATCACGCCGACTTCCACTTTCTGCTGTATTCGCTCCTGATTTCTTGGCCGGAATGGATCGATCATCGCCGGAAAAGCTCATCCTCGTCAACCGGGAACCAAACGGGATCGCATTCGTTACGATCAACCGACCGAAGTCGTTGAATTCGTTAACGAGAGCGATGATGGCAGATTTGGCTCAGGCGTTTAAGAGCTTGGACCGCGACGAGTCCGTGCGAGTTATCGTGCTCTCTGGATCAGGCCGAGCCTTCTGTTCCGGTGTAGACCTTACTGCGGCGGAGGATGTTTTCAAAGGAGATGTGAAGGATGTTGAAGCCGATCCGGTTGTGCAGATGGAACGCTGCCGGAAGCCGATAATCGGAGCGGTTGCAGGTTTCGCCATCACCGCTGGATTTGAGATCGCCTTGGCTTGCGACATACTCATAGCTGCCAAAGGAGCTAAGTTCATCGATACTCATGCTAGGTTTGGGATATTTCCTTCGTGGGGATTATCTCAGAAACTCCCGAGGATCATCGGAGTTAGCAAAGCTCGTGAAGTATCCTTAACGGCGATGCCTCTAACTGCAGAGGAAGCCGAGAGGAAGGGTTTGGTGAATCACGTGGTCGACGGAGGTGAATTGTTGAAGAAAACTCGAGAGATTGCGGAGGCCATCTTGAAGAACAATCAGGATTTGGTGTTGAGATACAAGTCCGTCATTAACGATGGCCTGAAGCTGGATCTTGGCCATGCTCTTGCGCTGGAGAAGGAGAGAGGTCATGCATATTACAATGGAATGACCAAAGAGCAATTCAAGAAAATGCAGGAATTCATAGCTGGTCGGAGCTCCAAGAAGCCTTCCAAGTTATAAGTCTAAACGATGTAGCTTACTGTCTGTTTCTACACTGCAACAATAATAAGAAGAACTTGCCTCCTAAACGTTTATGGTTGATTTGATATCACATTGAAAGGGTAAATTACAAGTAACAATTATTTGTGAAGGAAAAGTCCATAAGACTTTCAAAAGAAGCAAGACACGAAGCGGTACGCCAACGAGGATGTTCGGCTTCTTCTAAGGGTGGATGTTGAGATCCCACGTCGATTGGCCAGACACTTGGCGGTGTGCCAACGAGGACGTTGGACCCCTAAGAGGGGTGGATGGATTGTGAGATCCCACATCGGTCGGCCAGACACCGAGCGCTAAGCCAACAAGGACGTTGGGCCCCCAAAATGGGTGGAATTGTGAGATCTCATGTGTTGGTTGGAGAGGAGAACAAAACATTTCTTATAAGGGTGTGAAAATCTCTCTCTAACCTTTAATACCTTTAGCGGAAAGTTCAAAAAGGACAATACCGACTATTGGTGGGCTTGGGTTATTACATGAAAGCATTAACCCGCTGATATGTCTTTGAGTTTAGTCAGCAAATAACCCGAACCCGTTGAGTAGCAATGAAATTAAACCACAATTGAAAGAGAATGACTCGCCATACTGTATAGAAGTTTCTACTAGATTAGGCAAAAAAAAAAAAAACCTTCTACGTGGACCACTAGGCCTTGCAAAAGCAGGGGCATTGTTGGTCGTGCATGTAAGACAACGTTGTTGGGTATGTGAAGACGTGTCTCCAACAAGACAAGGTCACAGACTCTTATTGGGCCCAAATACTATTCTTTTAGCCCAAATAAAATGATTAGATGGGCCAAGAGGCCCAAGTCACAACCATAAAACTGACCCATACATGATAATGTTTGGAAAAATAGCACACATTTTGTGTATGGAGTAAAACAAATTATTATTTTCTGACCCATTATTTTCAGCTTTAATTGATAATAATTCTT

Coding sequence (CDS)

ATGGATCGATCATCGCCGGAAAAGCTCATCCTCGTCAACCGGGAACCAAACGGGATCGCATTCGTTACGATCAACCGACCGAAGTCGTTGAATTCGTTAACGAGAGCGATGATGGCAGATTTGGCTCAGGCGTTTAAGAGCTTGGACCGCGACGAGTCCGTGCGAGTTATCGTGCTCTCTGGATCAGGCCGAGCCTTCTGTTCCGGTGTAGACCTTACTGCGGCGGAGGATGTTTTCAAAGGAGATGTGAAGGATGTTGAAGCCGATCCGGTTGTGCAGATGGAACGCTGCCGGAAGCCGATAATCGGAGCGGTTGCAGGTTTCGCCATCACCGCTGGATTTGAGATCGCCTTGGCTTGCGACATACTCATAGCTGCCAAAGGAGCTAAGTTCATCGATACTCATGCTAGGTTTGGGATATTTCCTTCGTGGGGATTATCTCAGAAACTCCCGAGGATCATCGGAGTTAGCAAAGCTCGTGAAGTATCCTTAACGGCGATGCCTCTAACTGCAGAGGAAGCCGAGAGGAAGGGTTTGGTGAATCACGTGGTCGACGGAGGTGAATTGTTGAAGAAAACTCGAGAGATTGCGGAGGCCATCTTGAAGAACAATCAGGATTTGGTGTTGAGATACAAGTCCGTCATTAACGATGGCCTGAAGCTGGATCTTGGCCATGCTCTTGCGCTGGAGAAGGAGAGAGGTCATGCATATTACAATGGAATGACCAAAGAGCAATTCAAGAAAATGCAGGAATTCATAGCTGGTCGGAGCTCCAAGAAGCCTTCCAAGTTATAA

Protein sequence

MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALACDILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLVNHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERGHAYYNGMTKEQFKKMQEFIAGRSSKKPSKL

Homology

[Close]

BLAST of Cp4.1LG12g03160 vs. ExPASy Swiss-Prot
Match: Q6NL24 (Probable enoyl-CoA hydratase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECHIA PE=1 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 2.5e-112
Identity = 204/262 (77.86%), Postives = 230/262 (87.79%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MD++  E LI V +E  GIA +TINRPKSLNSLTRAMM DLA+AFK +D DESV+V++ +
Sbjct: 1   MDQTVSENLIQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFT 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           GSGR+FCSGVDLTAAE VFKGDVKD E DPVVQMER RKPIIGA+ GFAITAGFE+ALAC
Sbjct: 61  GSGRSFCSGVDLTAAESVFKGDVKDPETDPVVQMERLRKPIIGAINGFAITAGFELALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLV 180
           DIL+A++GAKF+DTHARFGIFPSWGLSQKL RIIG +KAREVSLT+MPLTA+ A + G V
Sbjct: 121 DILVASRGAKFMDTHARFGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAGKLGFV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERGHAYYNG 240
           NHVV+ GE LKK REIAEAI+KN Q +VLR KSVINDGLKLDLGHAL LEKER HAYY+G
Sbjct: 181 NHVVEEGEALKKAREIAEAIIKNEQGMVLRIKSVINDGLKLDLGHALTLEKERAHAYYSG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPS 263
           MTKEQF+KMQEFIAGR SKKPS
Sbjct: 241 MTKEQFRKMQEFIAGRGSKKPS 262

[Close]

BLAST of Cp4.1LG12g03160 vs. ExPASy Swiss-Prot
Match: P52046 (Short-chain-enoyl-CoA hydratase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=crt PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.6e-32
Identity = 86/242 (35.54%), Postives = 139/242 (57.44%), Query Frame = 0


Query: 19  IAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGSG-RAFCSGVDLTAAED 78
           +A VTINRPK+LN+L    + ++      ++ D  V  ++L+G+G ++F +G D++  ++
Sbjct: 14  VAVVTINRPKALNALNSDTLKEMDYVIGEIENDSEVLAVILTGAGEKSFVAGADISEMKE 73

Query: 79  V--FKGDVKDVEADPVV-QMERCRKPIIGAVAGFAITAGFEIALACDILIAAKGAKFIDT 138
           +   +G    +  + V  ++E   KP+I AV GFA+  G EIA++CDI IA+  A+F   
Sbjct: 74  MNTIEGRKFGILGNKVFRRLELLEKPVIAAVNGFALGGGCEIAMSCDIRIASSNARFGQP 133

Query: 139 HARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLVNHVVDGGELLKKTR 198
               GI P +G +Q+L R++G+  A+++  TA  + A+EA R GLVN VV+  EL+   +
Sbjct: 134 EVGLGITPGFGGTQRLSRLVGMGMAKQLIFTAQNIKADEALRIGLVNKVVEPSELMNTAK 193

Query: 199 EIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERGHAYYNGMTKEQFKKMQEFIA 257
           EIA  I+ N    V   K  IN G++ D+  ALA E E     ++  T++Q   M  FI 
Sbjct: 194 EIANKIVSNAPVAVKLSKQAINRGMQCDIDTALAFESEAFGECFS--TEDQKDAMTAFIE 253

[Close]

BLAST of Cp4.1LG12g03160 vs. ExPASy Swiss-Prot
Match: Q1ZXF1 (Probable enoyl-CoA hydratase, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=echs1 PE=3 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.6e-32
Identity = 89/242 (36.78%), Postives = 134/242 (55.37%), Query Frame = 0


Query: 19  IAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGSGRAFCSGVDLTAAEDV 78
           +  VT+NRPKSLN+L+  +++++  A K    D+ V  I+++GS +AF +G D+   E V
Sbjct: 34  VGLVTLNRPKSLNALSDGLISEINSAVKLFQEDKDVGSIIITGSEKAFAAGADIKEMEKV 93

Query: 79  FKGDVKDVEADPVVQ---MERCRKPIIGAVAGFAITAGFEIALACDILIAAKGAKFIDTH 138
              D      D + Q   + + RKPII AV G+A+  G E+A+ CDI+IA + A F    
Sbjct: 94  TLPDA--YNNDLLAQWHDLTKIRKPIIAAVNGYALGGGCELAMMCDIIIAGEKAVFGQPE 153

Query: 139 ARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLVNHVVDGGELLKKTRE 198
            + G  P  G +Q+L R IG SKA E+ LT   LTA EAE+ GLV+ VV   ELL +  +
Sbjct: 154 IKLGTIPGCGGTQRLIRAIGKSKAMELVLTGNNLTAVEAEKAGLVSKVVPVEELLTEATK 213

Query: 199 IAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERGHAYYNGMTKEQFKKMQEFIAG 258
           +AE I   +Q  V   K  +N   +L L   +  E+   H+ +   T +Q + M  F+  
Sbjct: 214 MAEKIASYSQLTVAMAKEAVNASYELTLQEGIRFERRMFHSTFG--THDQKEGMNAFVEK 271

[Close]

BLAST of Cp4.1LG12g03160 vs. ExPASy Swiss-Prot
Match: P64017 (Probable enoyl-CoA hydratase echA8 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=echA8 PE=3 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 5.7e-32
Identity = 85/249 (34.14%), Postives = 132/249 (53.01%), Query Frame = 0


Query: 10  ILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGSGRAFCSG 69
           ILV R+   +  +T+NRP++LN+L   +M ++  A   LD D  +  I+++GS +AF +G
Sbjct: 6   ILVERDQR-VGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAKAFAAG 65

Query: 70  VDLTAAEDVFKGDVKDVEADPV-VQMERCRKPIIGAVAGFAITAGFEIALACDILIAAKG 129
            D+    D+   D    +      ++   R P I AVAG+A+  G E+A+ CD+LIAA  
Sbjct: 66  ADIKEMADLTFADAFTADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADT 125

Query: 130 AKFIDTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLVNHVVDGGE 189
           AKF     + G+ P  G SQ+L R IG +KA ++ LT   + A EAER GLV+ VV   +
Sbjct: 126 AKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVVPADD 185

Query: 190 LLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERGHAYYNGMTKEQFKK 249
           LL + R  A  I + +       K  +N   +  L   L  E+   H+ +   T++Q + 
Sbjct: 186 LLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAF--ATEDQSEG 245

Query: 250 MQEFIAGRS 258
           M  FI  R+
Sbjct: 246 MAAFIEKRA 251

[Close]

BLAST of Cp4.1LG12g03160 vs. ExPASy Swiss-Prot
Match: P9WNN8 (Probable enoyl-CoA hydratase echA8 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=echA8 PE=3 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 5.7e-32
Identity = 85/249 (34.14%), Postives = 132/249 (53.01%), Query Frame = 0


Query: 10  ILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGSGRAFCSG 69
           ILV R+   +  +T+NRP++LN+L   +M ++  A   LD D  +  I+++GS +AF +G
Sbjct: 6   ILVERDQR-VGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAKAFAAG 65

Query: 70  VDLTAAEDVFKGDVKDVEADPV-VQMERCRKPIIGAVAGFAITAGFEIALACDILIAAKG 129
            D+    D+   D    +      ++   R P I AVAG+A+  G E+A+ CD+LIAA  
Sbjct: 66  ADIKEMADLTFADAFTADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADT 125

Query: 130 AKFIDTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLVNHVVDGGE 189
           AKF     + G+ P  G SQ+L R IG +KA ++ LT   + A EAER GLV+ VV   +
Sbjct: 126 AKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVVPADD 185

Query: 190 LLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERGHAYYNGMTKEQFKK 249
           LL + R  A  I + +       K  +N   +  L   L  E+   H+ +   T++Q + 
Sbjct: 186 LLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAF--ATEDQSEG 245

Query: 250 MQEFIAGRS 258
           M  FI  R+
Sbjct: 246 MAAFIEKRA 251

[Close]

BLAST of Cp4.1LG12g03160 vs. NCBI nr
Match: XP_023548460.1 (probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 510 bits (1313), Expect = 6.27e-182
Identity = 264/264 (100.00%), Postives = 264/264 (100.00%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERGHAYYNG 240
           NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERGHAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERGHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

[Close]

BLAST of Cp4.1LG12g03160 vs. NCBI nr
Match: XP_022959404.1 (probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita moschata])

HSP 1 Score: 505 bits (1300), Expect = 6.01e-180
Identity = 260/264 (98.48%), Postives = 263/264 (99.62%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFK+LDRDESVRVIVLS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKTLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFA+TAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAVTAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGV+KAREVSLTAMPLTAEEAERKGLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERGHAYYNG 240
           NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKER HAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

[Close]

BLAST of Cp4.1LG12g03160 vs. NCBI nr
Match: KAG6575117.1 (putative enoyl-CoA hydratase 1, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 503 bits (1295), Expect = 3.48e-179
Identity = 260/264 (98.48%), Postives = 262/264 (99.24%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFK+LDRDESVRVIVLS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKTLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGV+KAREVSLTAMPLTAEEAERKGLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERGHAYYNG 240
           NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKER HAYY G
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYTG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

[Close]

BLAST of Cp4.1LG12g03160 vs. NCBI nr
Match: XP_023006283.1 (probable enoyl-CoA hydratase 1, peroxisomal isoform X1 [Cucurbita maxima])

HSP 1 Score: 502 bits (1293), Expect = 7.01e-179
Identity = 260/264 (98.48%), Postives = 262/264 (99.24%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MDRSS EKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS
Sbjct: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           G+GRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC
Sbjct: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGV+KAREVSLTAMPLTAEEAERKGLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERGHAYYNG 240
           NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKER HAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

[Close]

BLAST of Cp4.1LG12g03160 vs. NCBI nr
Match: XP_023006284.1 (probable enoyl-CoA hydratase 1, peroxisomal isoform X2 [Cucurbita maxima])

HSP 1 Score: 499 bits (1285), Expect = 1.16e-177
Identity = 259/264 (98.11%), Postives = 261/264 (98.86%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MDRSS EKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS
Sbjct: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           G+GRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC
Sbjct: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLV 180
           DILIAAKGAKFIDTHARF IFPSWGLSQKLPRIIGV+KAREVSLTAMPLTAEEAERKGLV
Sbjct: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERGHAYYNG 240
           NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKER HAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

[Close]

BLAST of Cp4.1LG12g03160 vs. ExPASy TrEMBL
Match: A0A6J1H7Z0 (probable enoyl-CoA hydratase 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111460389 PE=4 SV=1)

HSP 1 Score: 505 bits (1300), Expect = 2.91e-180
Identity = 260/264 (98.48%), Postives = 263/264 (99.62%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFK+LDRDESVRVIVLS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKTLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFA+TAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAVTAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGV+KAREVSLTAMPLTAEEAERKGLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERGHAYYNG 240
           NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKER HAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

[Close]

BLAST of Cp4.1LG12g03160 vs. ExPASy TrEMBL
Match: A0A6J1L4H0 (probable enoyl-CoA hydratase 1, peroxisomal isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499066 PE=4 SV=1)

HSP 1 Score: 502 bits (1293), Expect = 3.40e-179
Identity = 260/264 (98.48%), Postives = 262/264 (99.24%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MDRSS EKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS
Sbjct: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           G+GRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC
Sbjct: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGV+KAREVSLTAMPLTAEEAERKGLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERGHAYYNG 240
           NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKER HAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

[Close]

BLAST of Cp4.1LG12g03160 vs. ExPASy TrEMBL
Match: A0A6J1L1R6 (probable enoyl-CoA hydratase 1, peroxisomal isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499066 PE=4 SV=1)

HSP 1 Score: 499 bits (1285), Expect = 5.63e-178
Identity = 259/264 (98.11%), Postives = 261/264 (98.86%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MDRSS EKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS
Sbjct: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           G+GRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC
Sbjct: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLV 180
           DILIAAKGAKFIDTHARF IFPSWGLSQKLPRIIGV+KAREVSLTAMPLTAEEAERKGLV
Sbjct: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERGHAYYNG 240
           NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKER HAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

[Close]

BLAST of Cp4.1LG12g03160 vs. ExPASy TrEMBL
Match: A0A6J1CAG2 (probable enoyl-CoA hydratase 1, peroxisomal isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009856 PE=4 SV=1)

HSP 1 Score: 478 bits (1229), Expect = 1.93e-169
Identity = 243/264 (92.05%), Postives = 257/264 (97.35%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MD SSPEKLILV+REPNGIAFVTINRPKSLNSLTR M+ DLA A KSLDRD+SVRVIVLS
Sbjct: 1   MDPSSPEKLILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           GSGRAFCSGVDL+AAE+VFKGDVKDVE+DPV+QMERCRKPIIGA+AGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLSAAEEVFKGDVKDVESDPVLQMERCRKPIIGAIAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKL RIIGV+KAREVSLTAMPLTAEEAER+GLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERGHAYYNG 240
           NHVV+GGELLKK RE+AEAILKNNQD+VLRYKSVINDGL+LDLGHALALEKERGHAYYNG
Sbjct: 181 NHVVEGGELLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

[Close]

BLAST of Cp4.1LG12g03160 vs. ExPASy TrEMBL
Match: A0A1S3C8X4 (probable enoyl-CoA hydratase 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103497788 PE=4 SV=1)

HSP 1 Score: 468 bits (1203), Expect = 1.77e-165
Identity = 239/264 (90.53%), Postives = 252/264 (95.45%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MDRSSPE LILVNRE NGIAF+TINRPKSLNSLTR MM DLA+AFKSLDRD+SVRVI+LS
Sbjct: 1   MDRSSPENLILVNRESNGIAFITINRPKSLNSLTRPMMIDLARAFKSLDRDDSVRVIILS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVE+DPV QME CRKPIIGA+AGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKL RIIGV+KAREVSLTAMPLTAEEAE++GLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERGHAYYNG 240
           N VV+G ELLKK RE+AEAILKNNQDLVLRYKSVINDGLKLDLG ALALEKER HAYYNG
Sbjct: 181 NQVVEGSELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264
           MTKEQF+KMQEFIAGRSSK+PSKL
Sbjct: 241 MTKEQFQKMQEFIAGRSSKRPSKL 264

[Close]

BLAST of Cp4.1LG12g03160 vs. TAIR 10
Match: AT4G16210.1 (enoyl-CoA hydratase/isomerase A )

HSP 1 Score: 406.4 bits (1043), Expect = 1.8e-113
Identity = 204/262 (77.86%), Postives = 230/262 (87.79%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MD++  E LI V +E  GIA +TINRPKSLNSLTRAMM DLA+AFK +D DESV+V++ +
Sbjct: 1   MDQTVSENLIQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFT 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           GSGR+FCSGVDLTAAE VFKGDVKD E DPVVQMER RKPIIGA+ GFAITAGFE+ALAC
Sbjct: 61  GSGRSFCSGVDLTAAESVFKGDVKDPETDPVVQMERLRKPIIGAINGFAITAGFELALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLV 180
           DIL+A++GAKF+DTHARFGIFPSWGLSQKL RIIG +KAREVSLT+MPLTA+ A + G V
Sbjct: 121 DILVASRGAKFMDTHARFGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAGKLGFV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERGHAYYNG 240
           NHVV+ GE LKK REIAEAI+KN Q +VLR KSVINDGLKLDLGHAL LEKER HAYY+G
Sbjct: 181 NHVVEEGEALKKAREIAEAIIKNEQGMVLRIKSVINDGLKLDLGHALTLEKERAHAYYSG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPS 263
           MTKEQF+KMQEFIAGR SKKPS
Sbjct: 241 MTKEQFRKMQEFIAGRGSKKPS 262

[Close]

BLAST of Cp4.1LG12g03160 vs. TAIR 10
Match: AT1G60550.1 (enoyl-CoA hydratase/isomerase D )

HSP 1 Score: 117.5 bits (293), Expect = 1.7e-26
Identity = 80/214 (37.38%), Postives = 113/214 (52.80%), Query Frame = 0


Query: 18  GIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGSG-RAFCSGVDLTAAE 77
           GIA +TINRP+  N+     + +L +AF     D SV VI+L+G G +AFCSG D     
Sbjct: 86  GIAKITINRPERRNAFRPQTVKELMRAFNDARDDSSVGVIILTGKGTKAFCSGGDQALRT 145

Query: 78  DVFKGDVKDV----EADPVVQMERCRKPIIGAVAGFAITAGFEIALACDILIAAKGAKFI 137
                D  DV      D  VQ+ R  KP+I  VAG+A+  G  + + CD+ IAA  A F 
Sbjct: 146 QDGYADPNDVGRLNVLDLQVQIRRLPKPVIAMVAGYAVGGGHILHMVCDLTIAADNAIFG 205

Query: 138 DTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLVNHVVDGGELLKK 197
            T  + G F +   S  + R++G  KARE+       TA EAE+ GL+N VV   +L K+
Sbjct: 206 QTGPKVGSFDAGYGSSIMSRLVGPKKAREMWFMTRFYTASEAEKMGLINTVVPLEDLEKE 265

Query: 198 TREIAEAILKNNQDLVLRYKSVINDGLKLDLGHA 227
           T +    IL+N+   +   K+ +N    +D GHA
Sbjct: 266 TVKWCREILRNSPTAIRVLKAALN---AVDDGHA 296

[Close]

BLAST of Cp4.1LG12g03160 vs. TAIR 10
Match: AT4G16800.1 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 109.0 bits (271), Expect = 5.9e-24
Identity = 72/235 (30.64%), Postives = 120/235 (51.06%), Query Frame = 0


Query: 4   SSPEKLILVNR---EPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVL- 63
           +SP + + +NR     +GI  V ++RP + N++ + M+  L  AF+S+ +D S RV+++ 
Sbjct: 37  TSPPEFVKLNRLSGSDSGIIEVNLDRPVTKNAINKEMLKSLQNAFESIHQDNSARVVMIR 96

Query: 64  SGSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQ---MERCRKPIIGAVAGFAITAGFEI 123
           S     FC+G DL     +   +V             +E    P I A+ G A+  G E+
Sbjct: 97  SLVPGVFCAGADLKERRTMSPSEVHTYVNSLRYMFSFIEALSIPTIAAIEGAALGGGLEM 156

Query: 124 ALACDILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAER 183
           ALACD+ I  + A F        I P  G +Q+L R++G S ++E+  T   + A EA  
Sbjct: 157 ALACDLRICGENAVFGLPETGLAIIPGAGGTQRLSRLVGRSVSKELIFTGRKIDAIEAAN 216

Query: 184 KGLVNHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEK 232
           KGLVN  V  GE  +K  E+A+ I +     +   K  I++G++ ++   L +E+
Sbjct: 217 KGLVNICVTAGEAHEKAIEMAQQINEKGPLAIKMAKKAIDEGIETNMASGLEVEE 271

[Close]

BLAST of Cp4.1LG12g03160 vs. TAIR 10
Match: AT5G43280.1 (delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 )

HSP 1 Score: 108.2 bits (269), Expect = 1.0e-23
Identity = 70/218 (32.11%), Postives = 109/218 (50.00%), Query Frame = 0


Query: 1   MDRSSPEKLILVNREPNGIAF-VTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVL 60
           M   S + L ++ +  +   F + INRP  LN+L+     +  +A  SLD++  V VI+L
Sbjct: 1   MTMESYKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIIL 60

Query: 61  SGSGRAFCSGVDLTAAEDV----------------FKGDVKDVEADPVVQMERCRKPIIG 120
           SG+G+ FCSG+DL +   +                 +  +K ++A  +  +E+CRKP+I 
Sbjct: 61  SGAGKHFCSGIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQA-AITAIEQCRKPVIA 120

Query: 121 AVAGFAITAGFEIALACDILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVSKAREVS 180
           A+ G  I  G ++  ACDI   ++ A F        I    G  Q+LP I+G + A E++
Sbjct: 121 AIHGACIGGGVDLITACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELA 180

Query: 181 LTAMPLTAEEAERKGLVNHVVDG-GELLKKTREIAEAI 201
           LTA   +  EA+  GLV+ V     EL      IAE I
Sbjct: 181 LTARRFSGSEAKDLGLVSKVFGSKSELDNGVTTIAEGI 217

[Close]

BLAST of Cp4.1LG12g03160 vs. TAIR 10
Match: AT4G29010.1 (Enoyl-CoA hydratase/isomerase family )

HSP 1 Score: 106.7 bits (265), Expect = 2.9e-23
Identity = 61/186 (32.80%), Postives = 109/186 (58.60%), Query Frame = 0


Query: 17  NGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGSGRAFCSGVDLTAAE 76
           +G+A +TI+ P  +NSL   +++ L + F+  ++   V+ IVL G+   F  G D+   +
Sbjct: 14  DGVAVITISNP-PVNSLASPIISGLKEKFRDANQRNDVKAIVLIGNNGRFSGGFDINVFQ 73

Query: 77  DVFK-GDVK---DVEADPVVQ-MERCRKPIIGAVAGFAITAGFEIALACDILIAAKGAKF 136
            V K GD+    +V  + V   ME  RKP++ AV G A+  G E+A+AC   +AA  A+ 
Sbjct: 74  QVHKTGDLSLMPEVSVELVCNLMEDSRKPVVAAVEGLALGGGLELAMACHARVAAPKAQL 133

Query: 137 IDTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLVNHVVDGGELLK 196
                  G+ P +G +Q+LPR++G++KA ++ L +  +++EE  + GL++ +V  G++L 
Sbjct: 134 GLPELTLGVIPGFGGTQRLPRLVGLAKATDMILLSKSISSEEGHKLGLIDALVPPGDVLS 193

Query: 197 KTREIA 198
            +R+ A
Sbjct: 194 TSRKWA 198

The following BLAST results are available for this feature:

BLAST of Cp4.1LG12g03160 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NL24	2.5e-112	77.86	Probable enoyl-CoA hydratase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=E...	[more]
P52046	2.6e-32	35.54	Short-chain-enoyl-CoA hydratase OS=Clostridium acetobutylicum (strain ATCC 824 /...	[more]
Q1ZXF1	2.6e-32	36.78	Probable enoyl-CoA hydratase, mitochondrial OS=Dictyostelium discoideum OX=44689...	[more]
P64017	5.7e-32	34.14	Probable enoyl-CoA hydratase echA8 OS=Mycobacterium bovis (strain ATCC BAA-935 /...	[more]
P9WNN8	5.7e-32	34.14	Probable enoyl-CoA hydratase echA8 OS=Mycobacterium tuberculosis (strain CDC 155...	[more]

BLAST of Cp4.1LG12g03160 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023548460.1	6.27e-182	100.00	probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita pepo subsp. pepo]	[more]
XP_022959404.1	6.01e-180	98.48	probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita moschata]	[more]
KAG6575117.1	3.48e-179	98.48	putative enoyl-CoA hydratase 1, peroxisomal, partial [Cucurbita argyrosperma sub...	[more]
XP_023006283.1	7.01e-179	98.48	probable enoyl-CoA hydratase 1, peroxisomal isoform X1 [Cucurbita maxima]	[more]
XP_023006284.1	1.16e-177	98.11	probable enoyl-CoA hydratase 1, peroxisomal isoform X2 [Cucurbita maxima]	[more]

BLAST of Cp4.1LG12g03160 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H7Z0	2.91e-180	98.48	probable enoyl-CoA hydratase 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1L4H0	3.40e-179	98.48	probable enoyl-CoA hydratase 1, peroxisomal isoform X1 OS=Cucurbita maxima OX=36...	[more]
A0A6J1L1R6	5.63e-178	98.11	probable enoyl-CoA hydratase 1, peroxisomal isoform X2 OS=Cucurbita maxima OX=36...	[more]
A0A6J1CAG2	1.93e-169	92.05	probable enoyl-CoA hydratase 1, peroxisomal isoform X1 OS=Momordica charantia OX...	[more]
A0A1S3C8X4	1.77e-165	90.53	probable enoyl-CoA hydratase 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103497...	[more]

BLAST of Cp4.1LG12g03160 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16210.1	1.8e-113	77.86	enoyl-CoA hydratase/isomerase A	[more]
AT1G60550.1	1.7e-26	37.38	enoyl-CoA hydratase/isomerase D	[more]
AT4G16800.1	5.9e-24	30.64	ATP-dependent caseinolytic (Clp) protease/crotonase family protein	[more]
AT5G43280.1	1.0e-23	32.11	delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1	[more]
AT4G29010.1	2.9e-23	32.80	Enoyl-CoA hydratase/isomerase family	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001753	Enoyl-CoA hydratase/isomerase	PFAM	PF00378	ECH_1	coord: 15..240 e-value: 3.0E-54 score: 184.0
None	No IPR available	GENE3D	3.90.226.10		coord: 10..260 e-value: 6.2E-69 score: 234.2
None	No IPR available	PANTHER	PTHR43802	ENOYL-COA HYDRATASE	coord: 6..260
None	No IPR available	CDD	cd06558	crotonase-like	coord: 10..200 e-value: 1.50292E-70 score: 213.576
IPR029045	ClpP/crotonase-like domain superfamily	SUPERFAMILY	52096	ClpP/crotonase	coord: 8..258

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG12g03160.1	Cp4.1LG12g03160.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009536	plastid
molecular_function	GO:0003824	catalytic activity