Cp4.1LG12g00270 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG12g00270
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description26S proteasome non-ATPase regulatory subunit 3 homolog A
LocationCp4.1LG12: 159257 .. 163459 (-)
RNA-Seq ExpressionCp4.1LG12g00270
SyntenyCp4.1LG12g00270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTATTCTCATGATAGCGTTGTTCTATGCCTTCGATGAAAGCCACCAGAGGTTTTCTTATTATCCACAGTTAGAATCGGACTCTTGTCGTATTAAAAAAATTATATATATAAAATTAAACTTTTTTTTTACAAAATTCAAAAATACCGATTAATAGCCAAAGCGACGTCGTTTTGTAACACTTCGATTCGCGCATTGTTTGCTCCTTTTCTCTTCTCACATTCACTGAGGGTTTGTCTTGCTGAAGAAGAAATTTCAAGAAACCCTTGCTTCTTCATCTCTGATCCATTTACACGGCGAAGATCTTGTTCTTTCTCTCGATTTCAGGTCAGATCCACTTGAACTATCGATTTGTTTCTTCCTTTTTGTTTCGCCACCGAAATTGTTGTTGTTTTACTTTTATGTAGTTTGTGAGATTTCCATACAGAACTTGCGAAGATGACCCAAGACGCCGAAATGAAGGAGCTTCCTGCTCCATTGCCGTCTTCGTCCTCTTCAACTTCCCCCTCAACTCTTCAGCGTAAGATAATTTTATCTGTATTTTTTAGTCTTGCTTTTGCGTTTTGTTTATTTGAATGCGACTCTCTACTGGGGATTAGGGGTTGGGATATCTTTCAATTTTTTTTATGTTTGTTCTTTAGGGCTTTCATTCAATCCTTAGAGTGCCTGGTTTTTAAATTTCTTAGTGGTTTCTTCTTTCAATCAGTTTTGAAAGAGATTGTATCGCTCATTGAGACTGGTGCCTATGCCCGGGAGGTTCGGCGCATTGTTCGTGCCATTCGTCTGACTATGGCCTTGAGGCGGAAGTTGAAGCCTTCTGTCCTTTCTCAATTTCTGAATCTTGTTCTGCCCCCAGGGTCAGAGGTGCACACTAGGTTATCGTCATTTCTCCCAAAGGTACTTAGAAATTATTAGGAAATTTTGATTTTCATCAGCATAATGCAATTTGAGTTATGGTTCATGGTAGTAGTTTTCTGAGCCTTCAAATTATTGCACAATTGCTTAGGAGGACGACTTTGAAATGGACGTTGACACGGCAACACAAGCTCCTGTCAAACACCCCCTGCCCGAGATAGAGATATTCTGCTACTTGATTGTGCTAATATTTTTTATTGATCAGAAGAAATATGACGATGTGAGGTTTTATGCAAAGGAATCAGTGTTTTTCATATTTTTCCTCAACTCTTGGCTAGTCATCCTGAGCACTAACTGAACATTTTCAGGCTAAGGCTTGCGCCTCAGCAAGCATCGCACGGCTCAAGAGCCTAAATAGGAGAACAGTCGATGTTTTAGCATCCAGGCTCTACTTCTATTACTCCCTCAGCTACGAACTCACTGGCGAACTTGCTGAAATAAGGGGGTGAGTTTTCATCTAAAACTGTAATAGGGTTATGGTAGTTAAGTTTACGCCTTTGTATGGGTCTAGTGGTTTTAATTCGGAGTATATAAATGAGGAGGAATGTTTAATTGAATATATTAATCTTTTAACGTGGAAAACTTTGATTGGCAGTAACCTCCTAGCACTGCATCGCATTGCAACCTTGCGCCATGATGAATTGGGTCAGGTAGTCCTTCAAACCTTTTCGGTGCATACCACCCGTGAGTTCATTATGGTGTCTTACGTATGGACAACAATGTCATGTAGGAAACCATCCTTAACCTTCTACTTCGTAACTACCTCCACTACAACCTCTATGATCAAGCAGAGAAGTTGAGGTCCAAGGCACCCCGATTTGAAGCTCACTCAAATCAGCAGGCGAGTTCTAANATTTCTTTTTGTCAAATGATTTTCAAATCTTCCTATCTTTCTCATCTATTTTTTTTTTCTTTTGATTTTGTTTTGTTGAGAGTATCTGTAGGTTCTTAAAAGATTTTTAATCGGTTCTTAGGTAGCATTGTTTTCCGTTGAAATGCTTGTAGCAGATGTAACTTGGCCATCATTTAAATAGTTTAGATCTTGTTTTGTATGCAATTTAAATTATGTTTTATGTTTTTGGTATCCATATTTTGGATTTGAAATTTTAAACTGCATAGTCAAAGAAAAGTAGAGAAAAGAAGACAAAGCCTTTAAAATTGTCGTATTTAACTTTATTAAGTCATTTCATTTTTGTTAAATAAAATAGGTAGAACATAATACTATTATAAATCATATGATATTGTAAAATAATAATTATACAACATTTCTAACATAAAACATGTACGTACATTATTTAACAATAGTTGATATTCAACCTAATAATTATTTGGTGACTACAACTATTTTTTTCTTTATAATTATTTAAAAAATTATATTTATAGTATATAAATATATTATCAAACTCATTTTGAACAACTGATTAAACTGGGGTCCATTTTCTAAATCTTCTACTAAACATATATTTGAAAACACTGAAAATGTAAAATATAACTTTGTTCTTCGTTGAATCCACTATTTTCAAGTTAATATTTTCAGATAACAAACTAAATGCACCATTAGGTAACTCGATGTTGAAATTGCTAAAGTTGACATGATTAGCCATGTGCTTATGAAGTTTTAGAATATAGCAGTTGTGTAGTATGTGAATTCATAAGAATCTAAGCATCATATTTGAAATTGTAGGGATGTTTTTCTGAGGTAGATTTTCTCGCCTTTTTTAGTATTAGAACTTAATTTATAAGCTACAATGCTAGATATCTCATAATGATTGGTTATTACAGTTCTGTAGGTACCTATTTTACCTTGGCAAGATCAGGACCATTCAGCTGGAGTATACAGATGCAAAGGAGTCTCTTCTACAAGCTGCTCGGAAAGCACCCATTGCAGCTCTTGGATTTCGAGTGCAATGCAACAAATGGGCAATCATTGTTCGGTTGCTGCTAGGAGAAATCCCTGAGAGGACCGTTTTCATGCAGAAAGGCATGGAGACAGCTCTGAGGCCATACTTTGAACTTACAAATGTAATTTTTCGATACTTTCCAGTTATTTTCTTCCATTAAGTTTAAAGTTTGTATGGAATCATTTCTTTTTTCTCTAACAAAAAATGAAATATTTGTTTCTTAGAAAGGTGAGGATGAGGGAAGCGGAAATGTTGTGGTAATGTAGGGAATCTTGTTAGTGGGAACCATATGTTAGTTGCCGATAGTATAGGTAATTACATGAATGGGTAGATAAGATGCAGGTGGAGGGATAACATAGGGGGGATATCTTTGGCTCAAGTGTTAATTTTGGTTTCGAATTTTCCCAAGTTCATGATCAAGTCAAGATAATGGCAGTGTGATTCCAGTTTATTATACTTTTTCTTAATGTATTGTTACCAATGTTTTTTTTTTTTTCCAGGCTGTGAGAATTGGTGACTTGGAGCTTTTCAGAACTGTTGCTGAGAAGTTCTCCAGTACTTTCAGCTCAGATCGAACCAACAACTTGATCGTGAGACTGCGACATAATGTCATTAGGACTGGGCTTCGCAACATTAGCATTTCTTATTCGCGTATTTCTCTGGCTGATGTTGCTAAAAAGCTTAGGTTGGACTCCCCAAATCCTGTTGCTGATGCTGAGAGTATTGTCTCGAAGGCAATCCGAGATGGAGCAATCGATGCAACTGTTGACCATGCGAATGGATGGATGGTATCCAAGGAGACTGGTGATATCTACTCGACCAATGAACCTCAGATGGCTTTCAACTCGAGAATTGCATTTTGCCTGAATATGCATAATGAAGCAGTTCGTGCCCTTCGGTTTCCACCAAATTCACACAAAGAAAAAGAAAGCGCTGAGAAAAGGAGAGAGAGACAACAGCAGGAGCAAGAACTTGCAAAGCACATAGCTGAAGAAGATGACGACGACTTTTAGCTTATTACATAATCTGGGAGCGATGGTATGAATATTTGGTGCATTAATGAATCAGAATTGCTGGGCAGAAAATTTTGATGCATTGGTTCAGATGAGTCCCGCCCATTTTCTTTATGTTTGTAATGCAACTCTTTCATTCGTGGAATGATGCCCATCTTATACTTACCCTTCTGAATTGTAACTCACCTTTGGGATTGCATCATGTGCAAGTACAATATTTGCTGGTAGGAAGATTGTTCTTGTAGTTGATGTAACGTTGGTCGGTTTTACTTCTACTCGCCATCTCGATGGCCTGCTGTTAATTTTAGCGTTGCATTTGGTAAATCATGGCCATATCTTGAAAGCATTGTTTTAAAATAACAGTGTCCCAGCTT

mRNA sequence

CTTTATTCTCATGATAGCGTTGTTCTATGCCTTCGATGAAAGCCACCAGAGGTTTTCTTATTATCCACAGTTAGAATCGGACTCTTGTCGTATTAAAAAAATTATATATATAAAATTAAACTTTTTTTTTACAAAATTCAAAAATACCGATTAATAGCCAAAGCGACGTCGTTTTGTAACACTTCGATTCGCGCATTGTTTGCTCCTTTTCTCTTCTCACATTCACTGAGGGTTTGTCTTGCTGAAGAAGAAATTTCAAGAAACCCTTGCTTCTTCATCTCTGATCCATTTACACGGCGAAGATCTTGTTCTTTCTCTCGATTTCAGAACTTGCGAAGATGACCCAAGACGCCGAAATGAAGGAGCTTCCTGCTCCATTGCCGTCTTCGTCCTCTTCAACTTCCCCCTCAACTCTTCAGCTTTTGAAAGAGATTGTATCGCTCATTGAGACTGGTGCCTATGCCCGGGAGGTTCGGCGCATTGTTCGTGCCATTCGTCTGACTATGGCCTTGAGGCGGAAGTTGAAGCCTTCTGTCCTTTCTCAATTTCTGAATCTTGTTCTGCCCCCAGGGTCAGAGGTGCACACTAGGTTATCGTCATTTCTCCCAAAGGAGGACGACTTTGAAATGGACGTTGACACGGCAACACAAGCTCCTGTCAAACACCCCCTGCCCGAGATAGAGATATTCTGCTACTTGATTGTGCTAATATTTTTTATTGATCAGAAGAAATATGACGATGCTAAGGCTTGCGCCTCAGCAAGCATCGCACGGCTCAAGAGCCTAAATAGGAGAACAGTCGATGTTTTAGCATCCAGGCTCTACTTCTATTACTCCCTCAGCTACGAACTCACTGGCGAACTTGCTGAAATAAGGGGTAACCTCCTAGCACTGCATCGCATTGCAACCTTGCGCCATGATGAATTGGGTCAGGAAACCATCCTTAACCTTCTACTTCGTAACTACCTCCACTACAACCTCTATGATCAAGCAGAGAAGTTGAGGTCCAAGGCACCCCGATTTGAAGCTCACTCAAATCAGCAGGCGAGTTCTAANATTTCTTTTTGTCAAATGATTTTCAAATCTTCCTATCTTTCTCATCTATTTTTTTTTTCTTTTGATTTTGTTTTGTTGAGAGTATCTTTCTGTAGGTACCTATTTTACCTTGGCAAGATCAGGACCATTCAGCTGGAGTATACAGATGCAAAGGAGTCTCTTCTACAAGCTGCTCGGAAAGCACCCATTGCAGCTCTTGGATTTCGAGTGCAATGCAACAAATGGGCAATCATTGTTCGGTTGCTGCTAGGAGAAATCCCTGAGAGGACCGTTTTCATGCAGAAAGGCATGGAGACAGCTCTGAGGCCATACTTTGAACTTACAAATGCTGTGAGAATTGGTGACTTGGAGCTTTTCAGAACTGTTGCTGAGAAGTTCTCCAGTACTTTCAGCTCAGATCGAACCAACAACTTGATCGTGAGACTGCGACATAATGTCATTAGGACTGGGCTTCGCAACATTAGCATTTCTTATTCGCGTATTTCTCTGGCTGATGTTGCTAAAAAGCTTAGGTTGGACTCCCCAAATCCTGTTGCTGATGCTGAGAGTATTGTCTCGAAGGCAATCCGAGATGGAGCAATCGATGCAACTGTTGACCATGCGAATGGATGGATGGTATCCAAGGAGACTGGTGATATCTACTCGACCAATGAACCTCAGATGGCTTTCAACTCGAGAATTGCATTTTGCCTGAATATGCATAATGAAGCAGTTCGTGCCCTTCGGTTTCCACCAAATTCACACAAAGAAAAAGAAAGCGCTGAGAAAAGGAGAGAGAGACAACAGCAGGAGCAAGAACTTGCAAAGCACATAGCTGAAGAAGATGACGACGACTTTTAGCTTATTACATAATCTGGGAGCGATGGTATGAATATTTGGTGCATTAATGAATCAGAATTGCTGGGCAGAAAATTTTGATGCATTGGTTCAGATGAGTCCCGCCCATTTTCTTTATGTTTGTAATGCAACTCTTTCATTCGTGGAATGATGCCCATCTTATACTTACCCTTCTGAATTGTAACTCACCTTTGGGATTGCATCATGTGCAAGTACAATATTTGCTGGTAGGAAGATTGTTCTTGTAGTTGATGTAACGTTGGTCGGTTTTACTTCTACTCGCCATCTCGATGGCCTGCTGTTAATTTTAGCGTTGCATTTGGTAAATCATGGCCATATCTTGAAAGCATTGTTTTAAAATAACAGTGTCCCAGCTT

Coding sequence (CDS)

ATGACCCAAGACGCCGAAATGAAGGAGCTTCCTGCTCCATTGCCGTCTTCGTCCTCTTCAACTTCCCCCTCAACTCTTCAGCTTTTGAAAGAGATTGTATCGCTCATTGAGACTGGTGCCTATGCCCGGGAGGTTCGGCGCATTGTTCGTGCCATTCGTCTGACTATGGCCTTGAGGCGGAAGTTGAAGCCTTCTGTCCTTTCTCAATTTCTGAATCTTGTTCTGCCCCCAGGGTCAGAGGTGCACACTAGGTTATCGTCATTTCTCCCAAAGGAGGACGACTTTGAAATGGACGTTGACACGGCAACACAAGCTCCTGTCAAACACCCCCTGCCCGAGATAGAGATATTCTGCTACTTGATTGTGCTAATATTTTTTATTGATCAGAAGAAATATGACGATGCTAAGGCTTGCGCCTCAGCAAGCATCGCACGGCTCAAGAGCCTAAATAGGAGAACAGTCGATGTTTTAGCATCCAGGCTCTACTTCTATTACTCCCTCAGCTACGAACTCACTGGCGAACTTGCTGAAATAAGGGGTAACCTCCTAGCACTGCATCGCATTGCAACCTTGCGCCATGATGAATTGGGTCAGGAAACCATCCTTAACCTTCTACTTCGTAACTACCTCCACTACAACCTCTATGATCAAGCAGAGAAGTTGAGGTCCAAGGCACCCCGATTTGAAGCTCACTCAAATCAGCAGGCGAGTTCTAANATTTCTTTTTGTCAAATGATTTTCAAATCTTCCTATCTTTCTCATCTATTTTTTTTTTCTTTTGATTTTGTTTTGTTGAGAGTATCTTTCTGTAGGTACCTATTTTACCTTGGCAAGATCAGGACCATTCAGCTGGAGTATACAGATGCAAAGGAGTCTCTTCTACAAGCTGCTCGGAAAGCACCCATTGCAGCTCTTGGATTTCGAGTGCAATGCAACAAATGGGCAATCATTGTTCGGTTGCTGCTAGGAGAAATCCCTGAGAGGACCGTTTTCATGCAGAAAGGCATGGAGACAGCTCTGAGGCCATACTTTGAACTTACAAATGCTGTGAGAATTGGTGACTTGGAGCTTTTCAGAACTGTTGCTGAGAAGTTCTCCAGTACTTTCAGCTCAGATCGAACCAACAACTTGATCGTGAGACTGCGACATAATGTCATTAGGACTGGGCTTCGCAACATTAGCATTTCTTATTCGCGTATTTCTCTGGCTGATGTTGCTAAAAAGCTTAGGTTGGACTCCCCAAATCCTGTTGCTGATGCTGAGAGTATTGTCTCGAAGGCAATCCGAGATGGAGCAATCGATGCAACTGTTGACCATGCGAATGGATGGATGGTATCCAAGGAGACTGGTGATATCTACTCGACCAATGAACCTCAGATGGCTTTCAACTCGAGAATTGCATTTTGCCTGAATATGCATAATGAAGCAGTTCGTGCCCTTCGGTTTCCACCAAATTCACACAAAGAAAAAGAAAGCGCTGAGAAAAGGAGAGAGAGACAACAGCAGGAGCAAGAACTTGCAAAGCACATAGCTGAAGAAGATGACGACGACTTTTAG

Protein sequence

MTQDAEMKELPAPLPSSSSSTSPSTLQLLKEIVSLIETGAYAREVRRIVRAIRLTMALRRKLKPSVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDVDTATQAPVKHPLPEIEIFCYLIVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLASRLYFYYSLSYELTGELAEIRGNLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQASSXISFCQMIFKSSYLSHLFFFSFDFVLLRVSFCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSPNPVADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF
Homology
BLAST of Cp4.1LG12g00270 vs. ExPASy Swiss-Prot
Match: P93768 (Probable 26S proteasome non-ATPase regulatory subunit 3 OS=Nicotiana tabacum OX=4097 GN=21D7 PE=2 SV=1)

HSP 1 Score: 805.8 bits (2080), Expect = 2.8e-232
Identity = 436/521 (83.69%), Postives = 458/521 (87.91%), Query Frame = 0

Query: 1   MTQDAEMKELPAPLPSSSSSTSPSTLQLLKEIVSLIETGAYAREVRRIVRAIRLTMALRR 60
           MTQD EMKE  AP  +S SST+PS    LKEI SLIETGAYAREVRRI RA+RLTMALR+
Sbjct: 1   MTQDVEMKEQAAPPSNSLSSTAPSIFHHLKEIASLIETGAYAREVRRISRAVRLTMALRK 60

Query: 61  KLKPSVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDVDTAT---QAPVKHPLPEIEIF 120
           KLK S LS FLN VL PGSEVH+RLSSFLPKED+ +M+VDTAT   QAP+K+PLPE+EI+
Sbjct: 61  KLKASSLSAFLNYVLVPGSEVHSRLSSFLPKEDEQDMEVDTATSGAQAPIKNPLPELEIY 120

Query: 121 CYLIVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLASRLYFYYSLSYELTGELAE 180
           CYL+VLIF IDQKKY++AKAC+SASIARLK++NRRTVDVLASRLYFYYSL YELTG+LAE
Sbjct: 121 CYLLVLIFLIDQKKYNEAKACSSASIARLKTVNRRTVDVLASRLYFYYSLCYELTGDLAE 180

Query: 181 IRGNLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQAS 240
           IRG LLALHRIATLRHDELGQET+LNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ  
Sbjct: 181 IRGYLLALHRIATLRHDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ-- 240

Query: 241 SXISFCQMIFKSSYLSHLFFFSFDFVLLRVSFCRYLFYLGKIRTIQLEYTDAKESLLQAA 300
                                          F RYLFYLGKIRTIQLEYTDAKESLLQAA
Sbjct: 241 -------------------------------FSRYLFYLGKIRTIQLEYTDAKESLLQAA 300

Query: 301 RKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLEL 360
           RKAP AALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGME ALRPYFELTNAVRIGDLEL
Sbjct: 301 RKAPQAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMEKALRPYFELTNAVRIGDLEL 360

Query: 361 FRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSPNPV 420
           FR VAEKFSSTFSSD TNNLIVRLRHNVIRTGLRNISISYSRISL DVAKKLRLDSPNPV
Sbjct: 361 FRKVAEKFSSTFSSDGTNNLIVRLRHNVIRTGLRNISISYSRISLVDVAKKLRLDSPNPV 420

Query: 421 ADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAV 480
           ADAESIVSKAIRDGAIDAT+DHANGWMVSKETGDIYSTNEPQ+AFNSRIAFCLNMHNEAV
Sbjct: 421 ADAESIVSKAIRDGAIDATLDHANGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNEAV 480

Query: 481 RALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 519
           RALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF
Sbjct: 481 RALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 488

BLAST of Cp4.1LG12g00270 vs. ExPASy Swiss-Prot
Match: Q06364 (Probable 26S proteasome non-ATPase regulatory subunit 3 OS=Daucus carota OX=4039 GN=21D7 PE=1 SV=2)

HSP 1 Score: 790.4 bits (2040), Expect = 1.2e-227
Identity = 420/522 (80.46%), Postives = 458/522 (87.74%), Query Frame = 0

Query: 1   MTQDAEMKELPAPLPSSS-SSTSPSTLQLLKEIVSLIETGAYAREVRRIVRAIRLTMALR 60
           MTQD EMKE+PAP PS+S ++ +PSTLQ LKEI SLIE+GAYAREVRRI+RA+RLT+ALR
Sbjct: 1   MTQDVEMKEVPAPAPSNSVTAATPSTLQHLKEIASLIESGAYAREVRRILRAVRLTIALR 60

Query: 61  RKLKPSVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDVDTATQAP---VKHPLPEIEI 120
           +KL  SV++ FLN  L PGSEVH RL+S+LPKED+ +M+VDTA  A     KH LPE+EI
Sbjct: 61  KKLNASVVNAFLNFSLVPGSEVHARLASYLPKEDEHDMEVDTAMSATTTLAKHSLPELEI 120

Query: 121 FCYLIVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLASRLYFYYSLSYELTGELA 180
           +CYL+VLIF IDQKKY +AKAC+SASIAR+K+LNRRTV+VLASRLYFYYSLSYELTG+LA
Sbjct: 121 YCYLLVLIFLIDQKKYSEAKACSSASIARVKNLNRRTVEVLASRLYFYYSLSYELTGDLA 180

Query: 181 EIRGNLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQA 240
           EIRGNLLALHRIATLRHDELGQET+LNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ 
Sbjct: 181 EIRGNLLALHRIATLRHDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ- 240

Query: 241 SSXISFCQMIFKSSYLSHLFFFSFDFVLLRVSFCRYLFYLGKIRTIQLEYTDAKESLLQA 300
                                           FCRYLFYLGKIRTIQLEYTDAKES LQA
Sbjct: 241 --------------------------------FCRYLFYLGKIRTIQLEYTDAKESXLQA 300

Query: 301 ARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLE 360
           ARKAP+AALGFRVQCNKWA+IVRLLLGEIPERTVFMQKGME ALRPYF LTNAVRIGDLE
Sbjct: 301 ARKAPVAALGFRVQCNKWAVIVRLLLGEIPERTVFMQKGMEKALRPYFXLTNAVRIGDLE 360

Query: 361 LFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSPNP 420
           LFR VA+KF+STF++DRT+NLIVRLRHNVIRTGLRNISISYSRISL DVA+KLRLDSPNP
Sbjct: 361 LFRXVADKFASTFTADRTHNLIVRLRHNVIRTGLRNISISYSRISLVDVARKLRLDSPNP 420

Query: 421 VADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEA 480
           VADAESIVSKAIRDGAIDAT+DHANGWMVSKETGDIYSTNEPQ AFNSRIAFCLNMHNEA
Sbjct: 421 VADAESIVSKAIRDGAIDATIDHANGWMVSKETGDIYSTNEPQAAFNSRIAFCLNMHNEA 480

Query: 481 VRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 519
           VRALRFP NSHK+KESAEKRRERQQQEQELAKHIAEEDDD+F
Sbjct: 481 VRALRFPANSHKDKESAEKRRERQQQEQELAKHIAEEDDDEF 489

BLAST of Cp4.1LG12g00270 vs. ExPASy Swiss-Prot
Match: Q9LNU4 (26S proteasome non-ATPase regulatory subunit 3 homolog A OS=Arabidopsis thaliana OX=3702 GN=RPN3A PE=1 SV=3)

HSP 1 Score: 737.3 bits (1902), Expect = 1.2e-211
Identity = 397/522 (76.05%), Postives = 438/522 (83.91%), Query Frame = 0

Query: 1   MTQDAEMKELPAPLPSSSSSTSPSTLQLLKEIVSLIETGAYAREVRRIVRAIRLTMALRR 60
           MTQD EMK+   P  S  SS S ST+Q LKEI +LI+TG+Y +EVRRI RA+RLT+ LR+
Sbjct: 1   MTQDVEMKDNNTPSQSIISS-STSTMQNLKEIAALIDTGSYTKEVRRIARAVRLTIGLRQ 60

Query: 61  KLKPSVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDVDTATQ----APVKHPLPEIEI 120
           KL  SVLS FL+  L PGSE H+RLSSF+PK D+ +M+VDTA+     AP KH   E+EI
Sbjct: 61  KLTGSVLSSFLDFALVPGSEAHSRLSSFVPKGDEHDMEVDTASSATQAAPSKHLPAELEI 120

Query: 121 FCYLIVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLASRLYFYYSLSYELTGELA 180
           +CY IVL+F IDQKKY++AKAC+SASIARLK++NRRT+DV+ASRLYFYYSLSYE TG+LA
Sbjct: 121 YCYFIVLLFLIDQKKYNEAKACSSASIARLKNVNRRTIDVIASRLYFYYSLSYEQTGDLA 180

Query: 181 EIRGNLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQA 240
           EIRG LLALH  ATLRHDELGQET+LNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ 
Sbjct: 181 EIRGTLLALHHSATLRHDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ- 240

Query: 241 SSXISFCQMIFKSSYLSHLFFFSFDFVLLRVSFCRYLFYLGKIRTIQLEYTDAKESLLQA 300
                                           FCRYLFYLGKIRTIQLEYTDAKESLLQA
Sbjct: 241 --------------------------------FCRYLFYLGKIRTIQLEYTDAKESLLQA 300

Query: 301 ARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLE 360
           ARKAPIAALGFR+QCNKWAI+VRLLLGEIPER++F QKGME ALRPYFELTNAVRIGDLE
Sbjct: 301 ARKAPIAALGFRIQCNKWAILVRLLLGEIPERSIFTQKGMEKALRPYFELTNAVRIGDLE 360

Query: 361 LFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSPNP 420
           LFRTV EKF  TF+ DRT+NLIVRLRHNVIRTGLRNISISYSRISL DVAKKLRL+S NP
Sbjct: 361 LFRTVQEKFLDTFAQDRTHNLIVRLRHNVIRTGLRNISISYSRISLPDVAKKLRLNSENP 420

Query: 421 VADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEA 480
           VADAESIV+KAIRDGAIDAT+DH NG MVSKETGDIYSTNEPQ AFNSRIAFCLNMHNEA
Sbjct: 421 VADAESIVAKAIRDGAIDATIDHKNGCMVSKETGDIYSTNEPQTAFNSRIAFCLNMHNEA 480

Query: 481 VRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 519
           VRALRFPPN+HKEKES EKRRER+QQE+ELAKH+AEEDDDDF
Sbjct: 481 VRALRFPPNTHKEKESDEKRRERKQQEEELAKHMAEEDDDDF 488

BLAST of Cp4.1LG12g00270 vs. ExPASy Swiss-Prot
Match: Q9LQR8 (26S proteasome non-ATPase regulatory subunit 3 homolog B OS=Arabidopsis thaliana OX=3702 GN=RPN3B PE=1 SV=2)

HSP 1 Score: 723.8 bits (1867), Expect = 1.4e-207
Identity = 387/521 (74.28%), Postives = 434/521 (83.30%), Query Frame = 0

Query: 1   MTQDAEMKELPAPLPSSSSSTSPSTLQLLKEIVSLIETGAYAREVRRIVRAIRLTMALRR 60
           MTQD EMK+   P  S  S+ + STLQ LKEI +LI+TG+Y +EVRRI RA+RLT+ LRR
Sbjct: 1   MTQDVEMKDNQTPTQSVVSAPT-STLQNLKEIAALIDTGSYTKEVRRIARAVRLTVGLRR 60

Query: 61  KLKPSVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDVDTA---TQAPVKHPLPEIEIF 120
           KL  SV+S FL+  L PGSE HTRLSSF+PK D+ +M+VDTA   +QAP KH   E+EI+
Sbjct: 61  KLTGSVISSFLDFALVPGSEAHTRLSSFVPKSDEHDMEVDTASSNSQAPPKHLPAELEIY 120

Query: 121 CYLIVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLASRLYFYYSLSYELTGELAE 180
           CY IVL+F IDQKKY++AK C++ASIARLKS+NRRTVDV+AS+LYFYYSLSYELT +LAE
Sbjct: 121 CYFIVLLFLIDQKKYNEAKVCSTASIARLKSVNRRTVDVIASKLYFYYSLSYELTNDLAE 180

Query: 181 IRGNLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQAS 240
           IR  LLALH  ATLRHDELGQET+LNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ  
Sbjct: 181 IRSTLLALHHSATLRHDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ-- 240

Query: 241 SXISFCQMIFKSSYLSHLFFFSFDFVLLRVSFCRYLFYLGKIRTIQLEYTDAKESLLQAA 300
                                          FCRYLFYLGKIRTIQLEYTDAKESLLQAA
Sbjct: 241 -------------------------------FCRYLFYLGKIRTIQLEYTDAKESLLQAA 300

Query: 301 RKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLEL 360
           RKAP+A+LGFR+QCNKWAIIVRLLLGEIPER++F QKGME  LRPYFELTNAVRIGDLEL
Sbjct: 301 RKAPVASLGFRIQCNKWAIIVRLLLGEIPERSIFTQKGMEKTLRPYFELTNAVRIGDLEL 360

Query: 361 FRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSPNPV 420
           F  + EKF+ TF+ DRT+NLIVRLRHNVIRTGLRNISISYSRISL DVA+KLRL+S NPV
Sbjct: 361 FGKIQEKFAKTFAEDRTHNLIVRLRHNVIRTGLRNISISYSRISLQDVAQKLRLNSANPV 420

Query: 421 ADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAV 480
           ADAESIV+KAIRDGAIDAT+DH NG MVSKETGDIYSTNEPQ AFNSRIAFCLNMHNEAV
Sbjct: 421 ADAESIVAKAIRDGAIDATIDHKNGCMVSKETGDIYSTNEPQTAFNSRIAFCLNMHNEAV 480

Query: 481 RALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 519
           RALRFPPN+H+EKES EKRRE +QQE+ELAK++AEEDDDDF
Sbjct: 481 RALRFPPNTHREKESEEKRREMKQQEEELAKYMAEEDDDDF 487

BLAST of Cp4.1LG12g00270 vs. ExPASy Swiss-Prot
Match: O43242 (26S proteasome non-ATPase regulatory subunit 3 OS=Homo sapiens OX=9606 GN=PSMD3 PE=1 SV=2)

HSP 1 Score: 370.2 bits (949), Expect = 3.9e-101
Identity = 227/525 (43.24%), Postives = 324/525 (61.71%), Query Frame = 0

Query: 2   TQDAEMKELPAPLPSSSSSTSPSTLQLLKEIVSLIETGAYAREVRRIVRAIRLTMALRRK 61
           T +A+ K   A    S       TL+ +KE V  +E     +E R ++RA+R+  +  R+
Sbjct: 48  TGEADGKTAAAAAEHSQRELDTVTLEDIKEHVKQLEKAVSGKEPRFVLRALRMLPSTSRR 107

Query: 62  LKPSVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDVD------TATQAPVKHPLPEIE 121
           L   VL + +       +     L  FL +  D E D+        A   P+   LPE+E
Sbjct: 108 LNHYVLYKAVQGFFTSNNATRDFLLPFLEEPMDTEADLQFRPRTGKAASTPL---LPEVE 167

Query: 122 IFCYLIVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLASRLYFYYSLSYELTGEL 181
            +  L+V+IF ++ K+Y +A+  +   + ++ + NRR +D++A++ Y+Y++  YE   +L
Sbjct: 168 AYLQLLVVIFMMNSKRYKEAQKISDDLMQKISTQNRRALDLVAAKCYYYHARVYEFLDKL 227

Query: 182 AEIRGNLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ 241
             +R  L A  R ATLRHD  GQ T+LNLLLRNYLHY+LYDQAEKL SK+   E  +N +
Sbjct: 228 DVVRSFLHARLRTATLRHDADGQATLLNLLLRNYLHYSLYDQAEKLVSKSVFPEQANNNE 287

Query: 242 ASSXISFCQMIFKSSYLSHLFFFSFDFVLLRVSFCRYLFYLGKIRTIQLEYTDAKESLLQ 301
                                            + RYL+Y G+I+ IQLEY++A+ ++  
Sbjct: 288 ---------------------------------WARYLYYTGRIKAIQLEYSEARRTMTN 347

Query: 302 AARKAP-IAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGD 361
           A RKAP   A+GF+   +K  I+V LLLGEIP+R  F Q  ++ +L PYF LT AVR G+
Sbjct: 348 ALRKAPQHTAVGFKQTVHKLLIVVELLLGEIPDRLQFRQPSLKRSLMPYFLLTQAVRTGN 407

Query: 362 LELFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSP 421
           L  F  V ++F   F +D T  LI+RLRHNVI+TG+R IS+SYSRISLAD+A+KL+LDSP
Sbjct: 408 LAKFNQVLDQFGEKFQADGTYTLIIRLRHNVIKTGVRMISLSYSRISLADIAQKLQLDSP 467

Query: 422 NPVADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHN 481
               DAE IV+KAIRDG I+A+++H  G++ SKE  DIYST EPQ+AF+ RI+FCL++HN
Sbjct: 468 E---DAEFIVAKAIRDGVIEASINHEKGYVQSKEMIDIYSTREPQLAFHQRISFCLDIHN 527

Query: 482 EAVRALRFPPNSH-KEKESAEKRRERQQQEQELAKHIAEEDDDDF 519
            +V+A+RFPP S+ K+ ESAE+RRER+QQ+ E AK +AE+DDD F
Sbjct: 528 MSVKAMRFPPKSYNKDLESAEERREREQQDLEFAKEMAEDDDDSF 533

BLAST of Cp4.1LG12g00270 vs. NCBI nr
Match: XP_023549020.1 (probable 26S proteasome non-ATPase regulatory subunit 3 [Cucurbita pepo subsp. pepo] >XP_023549021.1 probable 26S proteasome non-ATPase regulatory subunit 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 909 bits (2349), Expect = 0.0
Identity = 485/518 (93.63%), Postives = 485/518 (93.63%), Query Frame = 0

Query: 1   MTQDAEMKELPAPLPSSSSSTSPSTLQLLKEIVSLIETGAYAREVRRIVRAIRLTMALRR 60
           MTQDAEMKELPAPLPSSSSSTSPSTLQLLKEIVSLIETGAYAREVRRIVRAIRLTMALRR
Sbjct: 1   MTQDAEMKELPAPLPSSSSSTSPSTLQLLKEIVSLIETGAYAREVRRIVRAIRLTMALRR 60

Query: 61  KLKPSVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDVDTATQAPVKHPLPEIEIFCYL 120
           KLKPSVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDVDTATQAPVKHPLPEIEIFCYL
Sbjct: 61  KLKPSVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDVDTATQAPVKHPLPEIEIFCYL 120

Query: 121 IVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLASRLYFYYSLSYELTGELAEIRG 180
           IVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLASRLYFYYSLSYELTGELAEIRG
Sbjct: 121 IVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLASRLYFYYSLSYELTGELAEIRG 180

Query: 181 NLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQASSXI 240
           NLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ     
Sbjct: 181 NLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ----- 240

Query: 241 SFCQMIFKSSYLSHLFFFSFDFVLLRVSFCRYLFYLGKIRTIQLEYTDAKESLLQAARKA 300
                                       FCRYLFYLGKIRTIQLEYTDAKESLLQAARKA
Sbjct: 241 ----------------------------FCRYLFYLGKIRTIQLEYTDAKESLLQAARKA 300

Query: 301 PIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELFRT 360
           PIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELFRT
Sbjct: 301 PIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELFRT 360

Query: 361 VAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSPNPVADA 420
           VAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSPNPVADA
Sbjct: 361 VAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSPNPVADA 420

Query: 421 ESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAVRAL 480
           ESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAVRAL
Sbjct: 421 ESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAVRAL 480

Query: 481 RFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 518
           RFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF
Sbjct: 481 RFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 485

BLAST of Cp4.1LG12g00270 vs. NCBI nr
Match: KAG6574797.1 (putative 26S proteasome non-ATPase regulatory subunit 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 902 bits (2332), Expect = 0.0
Identity = 481/518 (92.86%), Postives = 482/518 (93.05%), Query Frame = 0

Query: 1   MTQDAEMKELPAPLPSSSSSTSPSTLQLLKEIVSLIETGAYAREVRRIVRAIRLTMALRR 60
           MTQDAEMKELPAPLPSSSSSTSPSTLQLLKEIVSLIETGAYAREVRR+VRAIRLTMALRR
Sbjct: 34  MTQDAEMKELPAPLPSSSSSTSPSTLQLLKEIVSLIETGAYAREVRRVVRAIRLTMALRR 93

Query: 61  KLKPSVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDVDTATQAPVKHPLPEIEIFCYL 120
           KLK SVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDVDTATQAPVKHPLPEIEIFCYL
Sbjct: 94  KLKASVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDVDTATQAPVKHPLPEIEIFCYL 153

Query: 121 IVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLASRLYFYYSLSYELTGELAEIRG 180
           IVLIFFIDQKKYDDAKACASASI RLKSLNRRTVDVLASRLYFYYSLSYELTGELAEIRG
Sbjct: 154 IVLIFFIDQKKYDDAKACASASITRLKSLNRRTVDVLASRLYFYYSLSYELTGELAEIRG 213

Query: 181 NLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQASSXI 240
           NLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ     
Sbjct: 214 NLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ----- 273

Query: 241 SFCQMIFKSSYLSHLFFFSFDFVLLRVSFCRYLFYLGKIRTIQLEYTDAKESLLQAARKA 300
                                       FCRYLFYLGKIRTIQLEYTDAKESLLQAARKA
Sbjct: 274 ----------------------------FCRYLFYLGKIRTIQLEYTDAKESLLQAARKA 333

Query: 301 PIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELFRT 360
           PIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELFRT
Sbjct: 334 PIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELFRT 393

Query: 361 VAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSPNPVADA 420
           VAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISL DVAKKLRLDSPNPVADA
Sbjct: 394 VAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLVDVAKKLRLDSPNPVADA 453

Query: 421 ESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAVRAL 480
           ESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAVRAL
Sbjct: 454 ESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAVRAL 513

Query: 481 RFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 518
           RFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF
Sbjct: 514 RFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 518

BLAST of Cp4.1LG12g00270 vs. NCBI nr
Match: KAG7013378.1 (putative 26S proteasome non-ATPase regulatory subunit 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 900 bits (2325), Expect = 0.0
Identity = 480/518 (92.66%), Postives = 483/518 (93.24%), Query Frame = 0

Query: 1   MTQDAEMKELPAPLPSSSSSTSPSTLQLLKEIVSLIETGAYAREVRRIVRAIRLTMALRR 60
           MTQDAE+KELPAPLPSSSSSTSPSTLQLLKEIVSLIETGAYAREVRR+VRAIRLTMALRR
Sbjct: 1   MTQDAEIKELPAPLPSSSSSTSPSTLQLLKEIVSLIETGAYAREVRRVVRAIRLTMALRR 60

Query: 61  KLKPSVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDVDTATQAPVKHPLPEIEIFCYL 120
           KLK SVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDVDTATQAPVKHPLPEIEIFCYL
Sbjct: 61  KLKASVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDVDTATQAPVKHPLPEIEIFCYL 120

Query: 121 IVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLASRLYFYYSLSYELTGELAEIRG 180
           IVLIFFIDQKKYDDAKACASASI RLKSLNRRTVDVLASRLYFYYSLSYELTGELAEIRG
Sbjct: 121 IVLIFFIDQKKYDDAKACASASITRLKSLNRRTVDVLASRLYFYYSLSYELTGELAEIRG 180

Query: 181 NLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQASSXI 240
           NLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQASS  
Sbjct: 181 NLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQASS-- 240

Query: 241 SFCQMIFKSSYLSHLFFFSFDFVLLRVSFCRYLFYLGKIRTIQLEYTDAKESLLQAARKA 300
                                         +YLFYLGKIRTIQLEYTDAKESLLQAARKA
Sbjct: 241 ------------------------------KYLFYLGKIRTIQLEYTDAKESLLQAARKA 300

Query: 301 PIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELFRT 360
           PIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELFRT
Sbjct: 301 PIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELFRT 360

Query: 361 VAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSPNPVADA 420
           VAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISL DVAKKLRLDSPNPVADA
Sbjct: 361 VAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLVDVAKKLRLDSPNPVADA 420

Query: 421 ESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAVRAL 480
           ESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAVRAL
Sbjct: 421 ESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAVRAL 480

Query: 481 RFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 518
           RFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF
Sbjct: 481 RFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 486

BLAST of Cp4.1LG12g00270 vs. NCBI nr
Match: XP_023006175.1 (probable 26S proteasome non-ATPase regulatory subunit 3 [Cucurbita maxima] >XP_023006176.1 probable 26S proteasome non-ATPase regulatory subunit 3 [Cucurbita maxima])

HSP 1 Score: 896 bits (2315), Expect = 0.0
Identity = 482/520 (92.69%), Postives = 483/520 (92.88%), Query Frame = 0

Query: 1   MTQDAEMKELPAPLPSSSSS--TSPSTLQLLKEIVSLIETGAYAREVRRIVRAIRLTMAL 60
           MTQDAEMKELPAPLPSSSSS  TSPSTLQLLKEIVSLIE GAYAREVRRIVRAIRLTMAL
Sbjct: 1   MTQDAEMKELPAPLPSSSSSSSTSPSTLQLLKEIVSLIEIGAYAREVRRIVRAIRLTMAL 60

Query: 61  RRKLKPSVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDVDTATQAPVKHPLPEIEIFC 120
           RRKLK SVLSQFLNLVLPPGSEV+TRLSSFLPKEDDFEMDVDTATQAPVKHPLPEIEIFC
Sbjct: 61  RRKLKASVLSQFLNLVLPPGSEVNTRLSSFLPKEDDFEMDVDTATQAPVKHPLPEIEIFC 120

Query: 121 YLIVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLASRLYFYYSLSYELTGELAEI 180
           YLIVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLASRLYFYYSLSYELTGELAEI
Sbjct: 121 YLIVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLASRLYFYYSLSYELTGELAEI 180

Query: 181 RGNLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQASS 240
           RGNLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ   
Sbjct: 181 RGNLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ--- 240

Query: 241 XISFCQMIFKSSYLSHLFFFSFDFVLLRVSFCRYLFYLGKIRTIQLEYTDAKESLLQAAR 300
                                         FCRYLFYLGKIRTIQLEYTDAKESLLQAAR
Sbjct: 241 ------------------------------FCRYLFYLGKIRTIQLEYTDAKESLLQAAR 300

Query: 301 KAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELF 360
           KAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELF
Sbjct: 301 KAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELF 360

Query: 361 RTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSPNPVA 420
           RTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSPNPVA
Sbjct: 361 RTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSPNPVA 420

Query: 421 DAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAVR 480
           DAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAVR
Sbjct: 421 DAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAVR 480

Query: 481 ALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 518
           ALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF
Sbjct: 481 ALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 487

BLAST of Cp4.1LG12g00270 vs. NCBI nr
Match: XP_022959338.1 (probable 26S proteasome non-ATPase regulatory subunit 3 isoform X1 [Cucurbita moschata] >XP_022959339.1 probable 26S proteasome non-ATPase regulatory subunit 3 isoform X1 [Cucurbita moschata] >XP_022959340.1 probable 26S proteasome non-ATPase regulatory subunit 3 isoform X2 [Cucurbita moschata] >XP_022959341.1 probable 26S proteasome non-ATPase regulatory subunit 3 isoform X2 [Cucurbita moschata])

HSP 1 Score: 882 bits (2278), Expect = 0.0
Identity = 476/539 (88.31%), Postives = 478/539 (88.68%), Query Frame = 0

Query: 1   MTQDAEMKELPAPLPSSSSSTSPSTLQ---------------------LLKEIVSLIETG 60
           MTQDAEMKELPAPLPSSSSSTSP                         +LKEIVSLIETG
Sbjct: 1   MTQDAEMKELPAPLPSSSSSTSPQLFSGFIQSLECLVFKFLSDFFFQSVLKEIVSLIETG 60

Query: 61  AYAREVRRIVRAIRLTMALRRKLKPSVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDV 120
           AYAREVRR+VRAIRLTMALRRKLK SVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDV
Sbjct: 61  AYAREVRRVVRAIRLTMALRRKLKASVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDV 120

Query: 121 DTATQAPVKHPLPEIEIFCYLIVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLAS 180
           DTATQAPVKHPLPEIEIFCYLIVLIFFIDQKKYDDAKACASASI RLKSLNRRTVDVLAS
Sbjct: 121 DTATQAPVKHPLPEIEIFCYLIVLIFFIDQKKYDDAKACASASITRLKSLNRRTVDVLAS 180

Query: 181 RLYFYYSLSYELTGELAEIRGNLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAE 240
           RLYFYYSLSYELTGELAEIRGNLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAE
Sbjct: 181 RLYFYYSLSYELTGELAEIRGNLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAE 240

Query: 241 KLRSKAPRFEAHSNQQASSXISFCQMIFKSSYLSHLFFFSFDFVLLRVSFCRYLFYLGKI 300
           KLRSKAPRFEAHSNQQ                                 FCRYLFYLGKI
Sbjct: 241 KLRSKAPRFEAHSNQQ---------------------------------FCRYLFYLGKI 300

Query: 301 RTIQLEYTDAKESLLQAARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETA 360
           RTIQLEYTDAKESLLQAARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETA
Sbjct: 301 RTIQLEYTDAKESLLQAARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETA 360

Query: 361 LRPYFELTNAVRIGDLELFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSR 420
           LRPYFELTNAVRIGDLELFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSR
Sbjct: 361 LRPYFELTNAVRIGDLELFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSR 420

Query: 421 ISLADVAKKLRLDSPNPVADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQ 480
           ISL DVAKKLRLDSPNPVADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQ
Sbjct: 421 ISLVDVAKKLRLDSPNPVADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQ 480

Query: 481 MAFNSRIAFCLNMHNEAVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 518
           MAFNSRIAFCLNMHNEAVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF
Sbjct: 481 MAFNSRIAFCLNMHNEAVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 506

BLAST of Cp4.1LG12g00270 vs. ExPASy TrEMBL
Match: A0A6J1KX20 (probable 26S proteasome non-ATPase regulatory subunit 3 OS=Cucurbita maxima OX=3661 GN=LOC111498988 PE=3 SV=1)

HSP 1 Score: 896 bits (2315), Expect = 0.0
Identity = 482/520 (92.69%), Postives = 483/520 (92.88%), Query Frame = 0

Query: 1   MTQDAEMKELPAPLPSSSSS--TSPSTLQLLKEIVSLIETGAYAREVRRIVRAIRLTMAL 60
           MTQDAEMKELPAPLPSSSSS  TSPSTLQLLKEIVSLIE GAYAREVRRIVRAIRLTMAL
Sbjct: 1   MTQDAEMKELPAPLPSSSSSSSTSPSTLQLLKEIVSLIEIGAYAREVRRIVRAIRLTMAL 60

Query: 61  RRKLKPSVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDVDTATQAPVKHPLPEIEIFC 120
           RRKLK SVLSQFLNLVLPPGSEV+TRLSSFLPKEDDFEMDVDTATQAPVKHPLPEIEIFC
Sbjct: 61  RRKLKASVLSQFLNLVLPPGSEVNTRLSSFLPKEDDFEMDVDTATQAPVKHPLPEIEIFC 120

Query: 121 YLIVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLASRLYFYYSLSYELTGELAEI 180
           YLIVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLASRLYFYYSLSYELTGELAEI
Sbjct: 121 YLIVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLASRLYFYYSLSYELTGELAEI 180

Query: 181 RGNLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQASS 240
           RGNLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ   
Sbjct: 181 RGNLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ--- 240

Query: 241 XISFCQMIFKSSYLSHLFFFSFDFVLLRVSFCRYLFYLGKIRTIQLEYTDAKESLLQAAR 300
                                         FCRYLFYLGKIRTIQLEYTDAKESLLQAAR
Sbjct: 241 ------------------------------FCRYLFYLGKIRTIQLEYTDAKESLLQAAR 300

Query: 301 KAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELF 360
           KAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELF
Sbjct: 301 KAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELF 360

Query: 361 RTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSPNPVA 420
           RTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSPNPVA
Sbjct: 361 RTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSPNPVA 420

Query: 421 DAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAVR 480
           DAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAVR
Sbjct: 421 DAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAVR 480

Query: 481 ALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 518
           ALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF
Sbjct: 481 ALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 487

BLAST of Cp4.1LG12g00270 vs. ExPASy TrEMBL
Match: A0A6J1H5N0 (probable 26S proteasome non-ATPase regulatory subunit 3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111460341 PE=3 SV=1)

HSP 1 Score: 882 bits (2278), Expect = 0.0
Identity = 476/539 (88.31%), Postives = 478/539 (88.68%), Query Frame = 0

Query: 1   MTQDAEMKELPAPLPSSSSSTSPSTLQ---------------------LLKEIVSLIETG 60
           MTQDAEMKELPAPLPSSSSSTSP                         +LKEIVSLIETG
Sbjct: 1   MTQDAEMKELPAPLPSSSSSTSPQLFSGFIQSLECLVFKFLSDFFFQSVLKEIVSLIETG 60

Query: 61  AYAREVRRIVRAIRLTMALRRKLKPSVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDV 120
           AYAREVRR+VRAIRLTMALRRKLK SVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDV
Sbjct: 61  AYAREVRRVVRAIRLTMALRRKLKASVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDV 120

Query: 121 DTATQAPVKHPLPEIEIFCYLIVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLAS 180
           DTATQAPVKHPLPEIEIFCYLIVLIFFIDQKKYDDAKACASASI RLKSLNRRTVDVLAS
Sbjct: 121 DTATQAPVKHPLPEIEIFCYLIVLIFFIDQKKYDDAKACASASITRLKSLNRRTVDVLAS 180

Query: 181 RLYFYYSLSYELTGELAEIRGNLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAE 240
           RLYFYYSLSYELTGELAEIRGNLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAE
Sbjct: 181 RLYFYYSLSYELTGELAEIRGNLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAE 240

Query: 241 KLRSKAPRFEAHSNQQASSXISFCQMIFKSSYLSHLFFFSFDFVLLRVSFCRYLFYLGKI 300
           KLRSKAPRFEAHSNQQ                                 FCRYLFYLGKI
Sbjct: 241 KLRSKAPRFEAHSNQQ---------------------------------FCRYLFYLGKI 300

Query: 301 RTIQLEYTDAKESLLQAARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETA 360
           RTIQLEYTDAKESLLQAARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETA
Sbjct: 301 RTIQLEYTDAKESLLQAARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETA 360

Query: 361 LRPYFELTNAVRIGDLELFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSR 420
           LRPYFELTNAVRIGDLELFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSR
Sbjct: 361 LRPYFELTNAVRIGDLELFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSR 420

Query: 421 ISLADVAKKLRLDSPNPVADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQ 480
           ISL DVAKKLRLDSPNPVADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQ
Sbjct: 421 ISLVDVAKKLRLDSPNPVADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQ 480

Query: 481 MAFNSRIAFCLNMHNEAVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 518
           MAFNSRIAFCLNMHNEAVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF
Sbjct: 481 MAFNSRIAFCLNMHNEAVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 506

BLAST of Cp4.1LG12g00270 vs. ExPASy TrEMBL
Match: A0A1S4E2Q6 (probable 26S proteasome non-ATPase regulatory subunit 3 OS=Cucumis melo OX=3656 GN=LOC103499019 PE=3 SV=1)

HSP 1 Score: 870 bits (2248), Expect = 0.0
Identity = 467/518 (90.15%), Postives = 472/518 (91.12%), Query Frame = 0

Query: 1   MTQDAEMKELPAPLPSSSSSTSPSTLQLLKEIVSLIETGAYAREVRRIVRAIRLTMALRR 60
           MTQDAEMKELPAPL SSSSS SPS LQ LKEIVSLIETGAYAREVRRIVRAIRLTMALRR
Sbjct: 1   MTQDAEMKELPAPLSSSSSSASPSALQNLKEIVSLIETGAYAREVRRIVRAIRLTMALRR 60

Query: 61  KLKPSVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDVDTATQAPVKHPLPEIEIFCYL 120
           KLK SVLSQFLNLVLP GSEVHTRLSSFLPKEDD EMDVDTATQAP KHPLPEIEI+ YL
Sbjct: 61  KLKASVLSQFLNLVLPTGSEVHTRLSSFLPKEDDCEMDVDTATQAPAKHPLPEIEIYSYL 120

Query: 121 IVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLASRLYFYYSLSYELTGELAEIRG 180
           IVLIF ID KKYDDAKACASASIARLK+LNRRTVDVLASRLYFYYSLSYELTG+LAEIRG
Sbjct: 121 IVLIFLIDHKKYDDAKACASASIARLKNLNRRTVDVLASRLYFYYSLSYELTGDLAEIRG 180

Query: 181 NLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQASSXI 240
           NLLALHRIATLRHDELGQET+LNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ     
Sbjct: 181 NLLALHRIATLRHDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ----- 240

Query: 241 SFCQMIFKSSYLSHLFFFSFDFVLLRVSFCRYLFYLGKIRTIQLEYTDAKESLLQAARKA 300
                                       FCRYLFYLGKIRTIQLEYTDAKESLLQAARKA
Sbjct: 241 ----------------------------FCRYLFYLGKIRTIQLEYTDAKESLLQAARKA 300

Query: 301 PIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELFRT 360
           PIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELFRT
Sbjct: 301 PIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELFRT 360

Query: 361 VAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSPNPVADA 420
           VAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISL DVAKKLRLDS NPVADA
Sbjct: 361 VAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLVDVAKKLRLDSANPVADA 420

Query: 421 ESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAVRAL 480
           ESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQ+AFNSRIAFCLNMHNEAVRAL
Sbjct: 421 ESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNEAVRAL 480

Query: 481 RFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 518
           RFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF
Sbjct: 481 RFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 485

BLAST of Cp4.1LG12g00270 vs. ExPASy TrEMBL
Match: A0A6J1DBS3 (probable 26S proteasome non-ATPase regulatory subunit 3 OS=Momordica charantia OX=3673 GN=LOC111019235 PE=3 SV=1)

HSP 1 Score: 868 bits (2244), Expect = 7.67e-316
Identity = 463/518 (89.38%), Postives = 474/518 (91.51%), Query Frame = 0

Query: 1   MTQDAEMKELPAPLPSSSSSTSPSTLQLLKEIVSLIETGAYAREVRRIVRAIRLTMALRR 60
           MTQDAEM ELPAPL SSSSSTSPSTLQ LKEIV L+ETGAYAREVRRIVRAIRLTMALRR
Sbjct: 1   MTQDAEMNELPAPLSSSSSSTSPSTLQHLKEIVLLLETGAYAREVRRIVRAIRLTMALRR 60

Query: 61  KLKPSVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDVDTATQAPVKHPLPEIEIFCYL 120
           KLK SVLSQFLNLVLPPGSEVH+RLSSFLPK+D +EMDVD+ATQAP KHPLPEIEIFCYL
Sbjct: 61  KLKASVLSQFLNLVLPPGSEVHSRLSSFLPKDDGYEMDVDSATQAPAKHPLPEIEIFCYL 120

Query: 121 IVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLASRLYFYYSLSYELTGELAEIRG 180
           IVLIF IDQKKYD+AKACASASIARLK+LNRRTVDVLASRLYFYYSLSYELTG+LAEIRG
Sbjct: 121 IVLIFLIDQKKYDEAKACASASIARLKNLNRRTVDVLASRLYFYYSLSYELTGDLAEIRG 180

Query: 181 NLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQASSXI 240
           NLLALHRIATLRHDELGQET+LNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ     
Sbjct: 181 NLLALHRIATLRHDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ----- 240

Query: 241 SFCQMIFKSSYLSHLFFFSFDFVLLRVSFCRYLFYLGKIRTIQLEYTDAKESLLQAARKA 300
                                       FCRYLFYLGKIRTIQLEYTDAKESLLQAARKA
Sbjct: 241 ----------------------------FCRYLFYLGKIRTIQLEYTDAKESLLQAARKA 300

Query: 301 PIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELFRT 360
           PIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELFRT
Sbjct: 301 PIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELFRT 360

Query: 361 VAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSPNPVADA 420
           VAEKFSSTFS+DRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLD  NPVADA
Sbjct: 361 VAEKFSSTFSTDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDCANPVADA 420

Query: 421 ESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAVRAL 480
           ESIVSKAIRDGAIDATVDHANGWMVSKETGDIY TNEPQ+AFNSRIAFCLNMHNEAVRAL
Sbjct: 421 ESIVSKAIRDGAIDATVDHANGWMVSKETGDIYLTNEPQIAFNSRIAFCLNMHNEAVRAL 480

Query: 481 RFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 518
           RFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF
Sbjct: 481 RFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 485

BLAST of Cp4.1LG12g00270 vs. ExPASy TrEMBL
Match: A0A6J1HL10 (probable 26S proteasome non-ATPase regulatory subunit 3 OS=Cucurbita moschata OX=3662 GN=LOC111464512 PE=3 SV=1)

HSP 1 Score: 867 bits (2240), Expect = 1.53e-315
Identity = 462/518 (89.19%), Postives = 475/518 (91.70%), Query Frame = 0

Query: 1   MTQDAEMKELPAPLPSSSSSTSPSTLQLLKEIVSLIETGAYAREVRRIVRAIRLTMALRR 60
           MTQDAEMKE+PAPLPS +SSTSPSTLQ LKEIVSLIETGAYAREVRRIVRAIRLTMALRR
Sbjct: 1   MTQDAEMKEIPAPLPSPTSSTSPSTLQHLKEIVSLIETGAYAREVRRIVRAIRLTMALRR 60

Query: 61  KLKPSVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDVDTATQAPVKHPLPEIEIFCYL 120
           KLK SVLSQFLNLVLP GSEV++RLSSFLPKE+D EMDVD+ TQAP KHPLPEIEIFCYL
Sbjct: 61  KLKASVLSQFLNLVLPSGSEVYSRLSSFLPKEEDCEMDVDSGTQAPSKHPLPEIEIFCYL 120

Query: 121 IVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLASRLYFYYSLSYELTGELAEIRG 180
           IVLIF IDQKKYDDAKACASASIARLK+LNRRTVDVL+SRLYFYYSLSYEL G+LAEIRG
Sbjct: 121 IVLIFLIDQKKYDDAKACASASIARLKNLNRRTVDVLSSRLYFYYSLSYELNGDLAEIRG 180

Query: 181 NLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQASSXI 240
           NLLALHRIATLRHDELGQET+LNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ     
Sbjct: 181 NLLALHRIATLRHDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ----- 240

Query: 241 SFCQMIFKSSYLSHLFFFSFDFVLLRVSFCRYLFYLGKIRTIQLEYTDAKESLLQAARKA 300
                                       FCRYLFYLGKIRTIQLEYTDAKESLLQAARKA
Sbjct: 241 ----------------------------FCRYLFYLGKIRTIQLEYTDAKESLLQAARKA 300

Query: 301 PIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLELFRT 360
           PIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETAL+PYFELTNAVRIGDLELFRT
Sbjct: 301 PIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALKPYFELTNAVRIGDLELFRT 360

Query: 361 VAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSPNPVADA 420
           VAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDS NPVADA
Sbjct: 361 VAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSANPVADA 420

Query: 421 ESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAVRAL 480
           ESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTN+PQ+AFNSRIAFCLNMHNEAVRAL
Sbjct: 421 ESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNDPQIAFNSRIAFCLNMHNEAVRAL 480

Query: 481 RFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 518
           RFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF
Sbjct: 481 RFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 485

BLAST of Cp4.1LG12g00270 vs. TAIR 10
Match: AT1G20200.1 (PAM domain (PCI/PINT associated module) protein )

HSP 1 Score: 737.3 bits (1902), Expect = 8.7e-213
Identity = 397/522 (76.05%), Postives = 438/522 (83.91%), Query Frame = 0

Query: 1   MTQDAEMKELPAPLPSSSSSTSPSTLQLLKEIVSLIETGAYAREVRRIVRAIRLTMALRR 60
           MTQD EMK+   P  S  SS S ST+Q LKEI +LI+TG+Y +EVRRI RA+RLT+ LR+
Sbjct: 1   MTQDVEMKDNNTPSQSIISS-STSTMQNLKEIAALIDTGSYTKEVRRIARAVRLTIGLRQ 60

Query: 61  KLKPSVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDVDTATQ----APVKHPLPEIEI 120
           KL  SVLS FL+  L PGSE H+RLSSF+PK D+ +M+VDTA+     AP KH   E+EI
Sbjct: 61  KLTGSVLSSFLDFALVPGSEAHSRLSSFVPKGDEHDMEVDTASSATQAAPSKHLPAELEI 120

Query: 121 FCYLIVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLASRLYFYYSLSYELTGELA 180
           +CY IVL+F IDQKKY++AKAC+SASIARLK++NRRT+DV+ASRLYFYYSLSYE TG+LA
Sbjct: 121 YCYFIVLLFLIDQKKYNEAKACSSASIARLKNVNRRTIDVIASRLYFYYSLSYEQTGDLA 180

Query: 181 EIRGNLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQA 240
           EIRG LLALH  ATLRHDELGQET+LNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ 
Sbjct: 181 EIRGTLLALHHSATLRHDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ- 240

Query: 241 SSXISFCQMIFKSSYLSHLFFFSFDFVLLRVSFCRYLFYLGKIRTIQLEYTDAKESLLQA 300
                                           FCRYLFYLGKIRTIQLEYTDAKESLLQA
Sbjct: 241 --------------------------------FCRYLFYLGKIRTIQLEYTDAKESLLQA 300

Query: 301 ARKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLE 360
           ARKAPIAALGFR+QCNKWAI+VRLLLGEIPER++F QKGME ALRPYFELTNAVRIGDLE
Sbjct: 301 ARKAPIAALGFRIQCNKWAILVRLLLGEIPERSIFTQKGMEKALRPYFELTNAVRIGDLE 360

Query: 361 LFRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSPNP 420
           LFRTV EKF  TF+ DRT+NLIVRLRHNVIRTGLRNISISYSRISL DVAKKLRL+S NP
Sbjct: 361 LFRTVQEKFLDTFAQDRTHNLIVRLRHNVIRTGLRNISISYSRISLPDVAKKLRLNSENP 420

Query: 421 VADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEA 480
           VADAESIV+KAIRDGAIDAT+DH NG MVSKETGDIYSTNEPQ AFNSRIAFCLNMHNEA
Sbjct: 421 VADAESIVAKAIRDGAIDATIDHKNGCMVSKETGDIYSTNEPQTAFNSRIAFCLNMHNEA 480

Query: 481 VRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 519
           VRALRFPPN+HKEKES EKRRER+QQE+ELAKH+AEEDDDDF
Sbjct: 481 VRALRFPPNTHKEKESDEKRRERKQQEEELAKHMAEEDDDDF 488

BLAST of Cp4.1LG12g00270 vs. TAIR 10
Match: AT1G75990.1 (PAM domain (PCI/PINT associated module) protein )

HSP 1 Score: 723.8 bits (1867), Expect = 9.9e-209
Identity = 387/521 (74.28%), Postives = 434/521 (83.30%), Query Frame = 0

Query: 1   MTQDAEMKELPAPLPSSSSSTSPSTLQLLKEIVSLIETGAYAREVRRIVRAIRLTMALRR 60
           MTQD EMK+   P  S  S+ + STLQ LKEI +LI+TG+Y +EVRRI RA+RLT+ LRR
Sbjct: 1   MTQDVEMKDNQTPTQSVVSAPT-STLQNLKEIAALIDTGSYTKEVRRIARAVRLTVGLRR 60

Query: 61  KLKPSVLSQFLNLVLPPGSEVHTRLSSFLPKEDDFEMDVDTA---TQAPVKHPLPEIEIF 120
           KL  SV+S FL+  L PGSE HTRLSSF+PK D+ +M+VDTA   +QAP KH   E+EI+
Sbjct: 61  KLTGSVISSFLDFALVPGSEAHTRLSSFVPKSDEHDMEVDTASSNSQAPPKHLPAELEIY 120

Query: 121 CYLIVLIFFIDQKKYDDAKACASASIARLKSLNRRTVDVLASRLYFYYSLSYELTGELAE 180
           CY IVL+F IDQKKY++AK C++ASIARLKS+NRRTVDV+AS+LYFYYSLSYELT +LAE
Sbjct: 121 CYFIVLLFLIDQKKYNEAKVCSTASIARLKSVNRRTVDVIASKLYFYYSLSYELTNDLAE 180

Query: 181 IRGNLLALHRIATLRHDELGQETILNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQAS 240
           IR  LLALH  ATLRHDELGQET+LNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ  
Sbjct: 181 IRSTLLALHHSATLRHDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ-- 240

Query: 241 SXISFCQMIFKSSYLSHLFFFSFDFVLLRVSFCRYLFYLGKIRTIQLEYTDAKESLLQAA 300
                                          FCRYLFYLGKIRTIQLEYTDAKESLLQAA
Sbjct: 241 -------------------------------FCRYLFYLGKIRTIQLEYTDAKESLLQAA 300

Query: 301 RKAPIAALGFRVQCNKWAIIVRLLLGEIPERTVFMQKGMETALRPYFELTNAVRIGDLEL 360
           RKAP+A+LGFR+QCNKWAIIVRLLLGEIPER++F QKGME  LRPYFELTNAVRIGDLEL
Sbjct: 301 RKAPVASLGFRIQCNKWAIIVRLLLGEIPERSIFTQKGMEKTLRPYFELTNAVRIGDLEL 360

Query: 361 FRTVAEKFSSTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSPNPV 420
           F  + EKF+ TF+ DRT+NLIVRLRHNVIRTGLRNISISYSRISL DVA+KLRL+S NPV
Sbjct: 361 FGKIQEKFAKTFAEDRTHNLIVRLRHNVIRTGLRNISISYSRISLQDVAQKLRLNSANPV 420

Query: 421 ADAESIVSKAIRDGAIDATVDHANGWMVSKETGDIYSTNEPQMAFNSRIAFCLNMHNEAV 480
           ADAESIV+KAIRDGAIDAT+DH NG MVSKETGDIYSTNEPQ AFNSRIAFCLNMHNEAV
Sbjct: 421 ADAESIVAKAIRDGAIDATIDHKNGCMVSKETGDIYSTNEPQTAFNSRIAFCLNMHNEAV 480

Query: 481 RALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF 519
           RALRFPPN+H+EKES EKRRE +QQE+ELAK++AEEDDDDF
Sbjct: 481 RALRFPPNTHREKESEEKRREMKQQEEELAKYMAEEDDDDF 487

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P937682.8e-23283.69Probable 26S proteasome non-ATPase regulatory subunit 3 OS=Nicotiana tabacum OX=... [more]
Q063641.2e-22780.46Probable 26S proteasome non-ATPase regulatory subunit 3 OS=Daucus carota OX=4039... [more]
Q9LNU41.2e-21176.0526S proteasome non-ATPase regulatory subunit 3 homolog A OS=Arabidopsis thaliana... [more]
Q9LQR81.4e-20774.2826S proteasome non-ATPase regulatory subunit 3 homolog B OS=Arabidopsis thaliana... [more]
O432423.9e-10143.2426S proteasome non-ATPase regulatory subunit 3 OS=Homo sapiens OX=9606 GN=PSMD3 ... [more]
Match NameE-valueIdentityDescription
XP_023549020.10.093.63probable 26S proteasome non-ATPase regulatory subunit 3 [Cucurbita pepo subsp. p... [more]
KAG6574797.10.092.86putative 26S proteasome non-ATPase regulatory subunit 3, partial [Cucurbita argy... [more]
KAG7013378.10.092.66putative 26S proteasome non-ATPase regulatory subunit 3 [Cucurbita argyrosperma ... [more]
XP_023006175.10.092.69probable 26S proteasome non-ATPase regulatory subunit 3 [Cucurbita maxima] >XP_0... [more]
XP_022959338.10.088.31probable 26S proteasome non-ATPase regulatory subunit 3 isoform X1 [Cucurbita mo... [more]
Match NameE-valueIdentityDescription
A0A6J1KX200.092.69probable 26S proteasome non-ATPase regulatory subunit 3 OS=Cucurbita maxima OX=3... [more]
A0A6J1H5N00.088.31probable 26S proteasome non-ATPase regulatory subunit 3 isoform X1 OS=Cucurbita ... [more]
A0A1S4E2Q60.090.15probable 26S proteasome non-ATPase regulatory subunit 3 OS=Cucumis melo OX=3656 ... [more]
A0A6J1DBS37.67e-31689.38probable 26S proteasome non-ATPase regulatory subunit 3 OS=Momordica charantia O... [more]
A0A6J1HL101.53e-31589.19probable 26S proteasome non-ATPase regulatory subunit 3 OS=Cucurbita moschata OX... [more]
Match NameE-valueIdentityDescription
AT1G20200.18.7e-21376.05PAM domain (PCI/PINT associated module) protein [more]
AT1G75990.19.9e-20974.28PAM domain (PCI/PINT associated module) protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 484..511
NoneNo IPR availableSMARTSM00753PINTcoord: 375..468
e-value: 1.5E-25
score: 100.9
NoneNo IPR availableGENE3D1.25.40.570coord: 284..471
e-value: 5.9E-8
score: 34.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 481..517
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 481..510
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availablePANTHERPTHR1075826S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3/COP9 SIGNALOSOME COMPLEX SUBUNIT 3coord: 1..246
coord: 267..517
NoneNo IPR availablePANTHERPTHR10758:SF1126S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3-RELATEDcoord: 1..246
coord: 267..517
IPR000717Proteasome component (PCI) domainSMARTSM00088PINT_4coord: 375..468
e-value: 1.5E-25
score: 100.9
IPR000717Proteasome component (PCI) domainPFAMPF01399PCIcoord: 341..446
e-value: 2.0E-22
score: 79.7
IPR000717Proteasome component (PCI) domainPROSITEPS50250PCIcoord: 269..450
score: 26.343119
IPR01358626S proteasome regulatory subunit, C-terminalPFAMPF08375Rpn3_Ccoord: 450..516
e-value: 1.6E-26
score: 92.6
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 377..451

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG12g00270.1Cp4.1LG12g00270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050790 regulation of catalytic activity
biological_process GO:0042176 regulation of protein catabolic process
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0008541 proteasome regulatory particle, lid subcomplex
cellular_component GO:0000502 proteasome complex
molecular_function GO:0030234 enzyme regulator activity