Cp4.1LG11g10490 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG11g10490
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionheparanase-like protein 3
LocationCp4.1LG11: 8737994 .. 8742945 (+)
RNA-Seq ExpressionCp4.1LG11g10490
SyntenyCp4.1LG11g10490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTGTTAAATGGTCTATTCATTTAACAAATTCAAACCCACAATTCTTCATTTCATAGTTTCCAAGATTGGTATTGTAAGTATATATTACAGTGTTCTTCCATCACTGTATACTCTGTTCTTCCTTTCATGGTTTCCTTTATCCTTTGATCTTTCGCCATTAGAGGAAGTTTATGGAAATGGGTTTTCGTTTTTGGGTTCTGGGGTTTTGTTTTTGGGTCTGTTTCTTCTTTAGCTGTTCTGGTTCTGTGAATTCCAAGGGCGAGGAAGGAGATGGGTTTCTGAGCGGGACGGTTTTGATCAATGGCAAATCCGCCATTGGAGGTATCGATGAAGATTTCGTTTGCGCTACGCTCGATTGGTGGCCTCCGGAGAAATGTGATTATGGAACCTGTAGTTGGGGCCAAGCTTCTCTGCTTAATCTGGTAATCTGATTTCGATACTCATTAATGGTGATGAACATTATCCGATATGTTTTTGATCGTACTCGTAAATTTTGGCATCTGGGTTTTGTTATTTGATCTTTTTCTTTAAGCTTCCTTTATGGGTTTTTGTGTTTATTTCTAAGGAACAGCGAAAGCATGTCGGTAGCTTTAGCTAAATACTCGTGGCTAGAAAAAATAGTGTTCATAAACTTAATTTTCAAATGCGTGACTAGAAAAACCTCTACTGCTGCTACACTGAATTGTTCTTATTGTTCTTCCAACTGCAGGACCTGGGCAGCAACATTCTATTAAATGCAATCAAAGGTTTGAATTCCTTGACCTCCTTGCATGTTTGGCTCATTATAACGGCTCAAGCCCATCGCTAGCCGGTGTTATCTTCTTTGGGCTTTTCCTTTTAGACTTTTCAAGGTTTTTAAAACGTGTCTGGTAGGAAGAGGTTTCCATATCCTTATAAAAGATGTTTCGTTCTCTTCCCCAACCGATGTGAGATCTCACAATCCACCCCCTTTAGGATCCAGCATTCTTGCTAACACACCGCCTCATATCCACCCCCTTCAAAACTGAAAGGGGGTGGATTGAGGAGTCCCACATCGATTAGAGAAGGGAACGAGTGCTAGTGAGGACTCTAGACCCCGAAGGGGGTGAGGAAGCTAAGCCCTAAAGGGGGTGGACATGAGGCAGTGGATTGTAAGATCTCACATCGGTTGGGGAGGAAAACATAGCATCCTTTATAAGGGTGTGAAAACCTCTCCTTCGCATACGCATTTTAAGAACTCTGAGGGAAAACCCGAAAGAGAAAAGCCTAAAGAAGACCGTATGTACCAACAGTGGCCTTTTAAGTACCTTCAAATCTCGACCTATACAAACTTTATTCTCTGCAAACATTAAAACTAATACCTTTTTTCATGAATGGTGCAGAATTTAAGCCCCTGAAGCTTAGATTGGGTGGCACTTTGCAAGATAAGCTCATATATGATACTGAAGATCACAAGCAGCCATGCATTTCTTTTAAAAGAAACACCAAAGAGTTGTTTGGCTTCACTCAAGGCTGCTTACCCCTTGACAGATGGGATAAACTAAATGACTTCTTCAAGAAAGGAGGGTAATCAAAATCTCATCCCATTTTGCTGTTATGAAGTTAGATTCCTCAAAAGTTGAATGCTGAAGTTGTGTAATCTTATACAGAGCTAAGATTATCTTTGGATTAAACGCTCTCAATGGACGAAAGTTTGCATCAGATGGGTCCGCTGTAGGGGCTTGGAATTACACGAACGCTGAATCGTTCGTGCGTTATACTGTCAAAAAGAACTACACAATCCATGGTTGGGAGTTTGGTAAGAACGGCTACTTGTATTCTTGACGCTTCATAGCGTAAGCGTATTATGGTTCGGTTAACAATTCATAATCGATTTAACTAAGTACAGACAGAGAATTCAAAACTGTTCATGTAACATATATTGTCCGTTTTGGCCTGTTACGTATTGCCATCAGTCTCACGGATTTAAAACATGTTTGCTAGGGAGAGATTTCCACACCCTTACGAGGAATACTTTGTTCTCCTCCCCAACCAATGTGGGATCTCACAATTCATCCCCCTTTAGGGTCCAACGTCCTCGTTGGCACTCGTTCCCTTCTCCAATAGATGTGGGACCCCCCAAATGCACCCCCTTTGAGGCATAGCGTCCTTGTTGGCACACCGCCTCATGTCCACCCCCTACAGGACTCATCCTCCTCGCTGACACATCGTCCAGTGTCTGGCTCTGATACTATTTGTAACTGTCCAAGCCCACCACTAACAGATATTGTCCTCTTTAAGCTTTCCCTTTTAGACTTTCCCTCAAGGTTTTTAAAACGTGTCTGCTAGGGAGAAGTTTCCACACCCTTATAAGAAATACTTTGTTCTCCTCCCCAACCAATGTGGGATCTCACAATCCATCCCCCTTTAGGGTCCAGTGTCCTCGCTAGCACTCATTTCTTTCTCCAATCGATGTGGGACACCTCAATTTTGGGGCACACCACCTCATGTCCATCCCCTACGGGACTCATCCTCCTCGCTAGCACATCGCCCCGTCTCTGGCTCTGATACTATTTGCAACGGTCCAAGCCCACCACTAGCAGATATTATCCTCTTTAGGTTTTTCCTTTCAGACTTTCCCTCAAGGTTTTTAAAACGTGTATGCTAGGAAGAAGTTTCCACACCCTTAAAAAGAATGTTTCGTTCTCCTCTCCAACCTATGTGGGGTCTCCCAGTTCATCCCGAACGACTCGTATTTGACAATGGATAAGGATTCTTCTGTTCTAATCTATGATTGATTTCCATTTTCTTATGCTCAGGGAATGAATTGTGTGGGAGTGGAGTTGGAACAAGAATTTCAGCAGAACAGTATGCTTCTGATACAATTGTTTTTCAGAAGATGGTCCAAAGCATCTACAACGGTGTCCAAACTAAGCCATTAACCATAGCACCAGGAGGATTCTTTGATGAAAATTGGTTCAAAGAATTCGTTGATAAAACTACCCCAACACTGGATGCTGTCACACACCATATATACAATCTAGGAGCAGGTATCAACCACTAGCCAAGTAAGACCTTTATGAAGTTTCAAAAAGTGGGTTAACACTGGAGCTTTTTCTTACTAACAGGAGTCGATGAGCATCTTGTCGAAAGGATTCTTAACCCGTCCTATCTCAATGGCATGGTCGATACATTTTACAAGCTTCATGAGGTCCTTAGAAACTCGCGAAGTTCAGCTAAAGCGTGGGTTGGGGAAGCAGGAGGGGCTTACAGCAGTGGGCATAATCTGGTCTCTAATGCATTCGTCTCGAGTTTCTGGTAATCTAGGCTCCGAAAAACAGGAACGTATGGCTCTTTTAAGACCTTCATAATAAATAGCACTCTTTTATGTGTGTGTTTTATTCAGGTACTTGGACCAGCTCGGTATGGCAGCCACTTTTGATACGAAAACATACTGCAGACAGACGTTGGTTGGTGGAAACTATGGTTTACTGAACACGACAACCTTTGAACCGAATCCAGATTATTACAGGTGAAAGACTAGGCAAATATTTCTAACCTTAAACTCTTCGCTCCTCCCCGGATGCTATTAGACCATTATCTAACAATCAGCTTGCTTGTTTCTTTTCTCTGTTTTCATTTCAGCGCACTCCTATGGCATCGCTTGATGGGAAGAAATGTTCTGTCCACGAGCTTTAACGGGACAAAGAAAATACGAGCCTATGCACATTGTTCTAAGCAATCGGTAAGTAGTAGTACATACTGTAACAGCCCAAGCCCACCGCTAGCAAATATTGTCCTCCTTGAGCTTTCCCTTTCGGGCTTCCTCTCAAGGTTTTTAAAACACGTCTCTAAGGGAGGTGGATTGTGAAATCTCACCTCCGTTGGAGAGGAAAACGAAGCATTCCTTATAAGGGTGTGGAAACCTCTCCCTAGCAAACACGTTCTAGAAACCTTGAGGGAAAGCCCGAAAGAAAAAGCTCAAAAAGGACAATATCTACTAGCAGAAATGCCACCAGTTTTCAACTCGAATTCTAACTCTCAATCTTACAGAAAGTTTTCAACTCGAATTCTAACTCTCAATCTTACAGAAAGGAATCACACTACTATTGCTCAACCTAGATGGAAACACCACAGTTCATGCTGCGGTAGCATTCAACAGAACGAGCGGGTTACACTATAAACACAGATCCTATAAGAATAAGCAAAAGGTTGTTCGGACATCTCGACCTTACGGTCGTGAAGGCGAAGCCTTCAGAGAGGAATATCATATCACAGCCAAGGATGGGAACCTGCATAGTCAAACAGTGTTGCTAAATGGGAAGATTTTGTCTGTAAATTCATCAGGGAACATACCTTCATTGCAGCCTAAGCAAGTGAATTCATCTGAACCAATAATGGTGGCACCCTTTTCGATCGTGTTCGTTCACATACCGAATATTGTTCTCCCTGCTTGTGGCAATGTATGGAACTGAAAATCAGTTCCAGATTGGTGATTTTCAAATCAGAGAACAATTCTAATAAGAGTCTTTGAATCAGAAGATACACATTTTACAGAACATTTGCAGCTTTAGCAGGATTACTATGACTAACAATGNGGAACTGAAAATCAGTTCCAGATTGGTGATTTTCGAATCAGAGAACAATTCTAATAAGAGTCTTTGAATCAGAAGATACACATTTTACAGAACATTTGCAGCTTTAGCAGGATTACTATGACTAACAATGAGAAACAAAATCTCTACATGATCAATAAAATGCATATTTGTGGGGAATATACAAGTATTCACTATGGGTATACAACTGAAGAACCTGATGATGAAATGCAGTCGATGGCTCGTAATGGCGTTCTTCTCGGAAAATTCATGTAATGTAATACTACTCATTCTTCGAAAAGGTTTTAGGTTTACTGCTGGCAAAGACCAACCAAACAATTGTCCCGGTCAAGTAGAAGAAGATCGATGGGGCAAACAATGAGATCTACACATAACA

mRNA sequence

AATTGTTAAATGGTCTATTCATTTAACAAATTCAAACCCACAATTCTTCATTTCATAGTTTCCAAGATTGGTATTGTAAGTATATATTACAGTGTTCTTCCATCACTGTATACTCTGTTCTTCCTTTCATGGTTTCCTTTATCCTTTGATCTTTCGCCATTAGAGGAAGTTTATGGAAATGGGTTTTCGTTTTTGGGTTCTGGGGTTTTGTTTTTGGGTCTGTTTCTTCTTTAGCTGTTCTGGTTCTGTGAATTCCAAGGGCGAGGAAGGAGATGGGTTTCTGAGCGGGACGGTTTTGATCAATGGCAAATCCGCCATTGGAGGTATCGATGAAGATTTCGTTTGCGCTACGCTCGATTGGTGGCCTCCGGAGAAATGTGATTATGGAACCTGTAGTTGGGGCCAAGCTTCTCTGCTTAATCTGCTTCCTTTATGGGTTTTTGTGTTTATTTCTAAGGAACAGCGAAAGCATGTCGAATTTAAGCCCCTGAAGCTTAGATTGGGTGGCACTTTGCAAGATAAGCTCATATATGATACTGAAGATCACAAGCAGCCATGCATTTCTTTTAAAAGAAACACCAAAGAGTTGTTTGGCTTCACTCAAGGCTGCTTACCCCTTGACAGATGGGATAAACTAAATGACTTCTTCAAGAAAGGAGGAGCTAAGATTATCTTTGGATTAAACGCTCTCAATGGACGAAAGTTTGCATCAGATGGGTCCGCTGTAGGGGCTTGGAATTACACGAACGCTGAATCGTTCAAAGGAATCACACTACTATTGCTCAACCTAGATGGAAACACCACAGTTCATGCTGCGGTAGCATTCAACAGAACGAGCGGGTTACACTATAAACACAGATCCTATAAGAATAAGCAAAAGGTTGTTCGGACATCTCGACCTTACGGTCGTGAAGGCGAAGCCTTCAGAGAGGAATATCATATCACAGCCAAGGATGGGAACCTGCATAGTCAAACAGTGTTGCTAAATGGGAAGATTTTGTCTGTAAATTCATCAGGGAACATACCTTCATTGCAGCCTAAGCAAGTGAATTCATCTGAACCAATAATGGTGGCACCCTTTTCGATCGTGTTCGTTCACATACCGAATATTGTTCTCCCTGCTTGTGGCAATGTATGGAACTGAAAATCAGTTCCAGATTGGTGATTTTCAAATCAGAGAACAATTCTAATAAGAGTCTTTGAATCAGAAGATACACATTTTACAGAACATTTGCAGCTTTAGCAGGATTACTATGACTAACAATGNGGAACTGAAAATCAGTTCCAGATTGGTGATTTTCGAATCAGAGAACAATTCTAATAAGAGTCTTTGAATCAGAAGATACACATTTTACAGAACATTTGCAGCTTTAGCAGGATTACTATGACTAACAATGAGAAACAAAATCTCTACATGATCAATAAAATGCATATTTGTGGGGAATATACAAGTATTCACTATGGGTATACAACTGAAGAACCTGATGATGAAATGCAGTCGATGGCTCGTAATGGCGTTCTTCTCGGAAAATTCATGTAATGTAATACTACTCATTCTTCGAAAAGGTTTTAGGTTTACTGCTGGCAAAGACCAACCAAACAATTGTCCCGGTCAAGTAGAAGAAGATCGATGGGGCAAACAATGAGATCTACACATAACA

Coding sequence (CDS)

ATGGAAATGGGTTTTCGTTTTTGGGTTCTGGGGTTTTGTTTTTGGGTCTGTTTCTTCTTTAGCTGTTCTGGTTCTGTGAATTCCAAGGGCGAGGAAGGAGATGGGTTTCTGAGCGGGACGGTTTTGATCAATGGCAAATCCGCCATTGGAGGTATCGATGAAGATTTCGTTTGCGCTACGCTCGATTGGTGGCCTCCGGAGAAATGTGATTATGGAACCTGTAGTTGGGGCCAAGCTTCTCTGCTTAATCTGCTTCCTTTATGGGTTTTTGTGTTTATTTCTAAGGAACAGCGAAAGCATGTCGAATTTAAGCCCCTGAAGCTTAGATTGGGTGGCACTTTGCAAGATAAGCTCATATATGATACTGAAGATCACAAGCAGCCATGCATTTCTTTTAAAAGAAACACCAAAGAGTTGTTTGGCTTCACTCAAGGCTGCTTACCCCTTGACAGATGGGATAAACTAAATGACTTCTTCAAGAAAGGAGGAGCTAAGATTATCTTTGGATTAAACGCTCTCAATGGACGAAAGTTTGCATCAGATGGGTCCGCTGTAGGGGCTTGGAATTACACGAACGCTGAATCGTTCAAAGGAATCACACTACTATTGCTCAACCTAGATGGAAACACCACAGTTCATGCTGCGGTAGCATTCAACAGAACGAGCGGGTTACACTATAAACACAGATCCTATAAGAATAAGCAAAAGGTTGTTCGGACATCTCGACCTTACGGTCGTGAAGGCGAAGCCTTCAGAGAGGAATATCATATCACAGCCAAGGATGGGAACCTGCATAGTCAAACAGTGTTGCTAAATGGGAAGATTTTGTCTGTAAATTCATCAGGGAACATACCTTCATTGCAGCCTAAGCAAGTGAATTCATCTGAACCAATAATGGTGGCACCCTTTTCGATCGTGTTCGTTCACATACCGAATATTGTTCTCCCTGCTTGTGGCAATGTATGGAACTGA

Protein sequence

MEMGFRFWVLGFCFWVCFFFSCSGSVNSKGEEGDGFLSGTVLINGKSAIGGIDEDFVCATLDWWPPEKCDYGTCSWGQASLLNLLPLWVFVFISKEQRKHVEFKPLKLRLGGTLQDKLIYDTEDHKQPCISFKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFASDGSAVGAWNYTNAESFKGITLLLLNLDGNTTVHAAVAFNRTSGLHYKHRSYKNKQKVVRTSRPYGREGEAFREEYHITAKDGNLHSQTVLLNGKILSVNSSGNIPSLQPKQVNSSEPIMVAPFSIVFVHIPNIVLPACGNVWN
Homology
BLAST of Cp4.1LG11g10490 vs. ExPASy Swiss-Prot
Match: Q9FZP1 (Heparanase-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g34940 PE=2 SV=2)

HSP 1 Score: 254.6 bits (649), Expect = 1.5e-66
Identity = 175/544 (32.17%), Postives = 222/544 (40.81%), Query Frame = 0

Query: 9   VLGFCFWVCFFFSCSGSVNSKGEEGDGFLSGTVLINGKSAIGGIDEDFVCATLDWWPPEK 68
           +L    ++C F     +V+S  EE     +GTV + G++A+G IDEDF+CATLDWWPPEK
Sbjct: 6   ILAIVLFLCVFQFLDCTVSSAVEE-----NGTVFVYGRAAVGTIDEDFICATLDWWPPEK 65

Query: 69  CDYGTCSWGQASLLNLLPLWVFVFISKEQRKHVEFKPLKLRLGGTLQDKLIYDTEDHKQP 128
           CDYG+CSW  AS+LNL    V +     Q     F PLK+R+GGTLQD +IY+T D KQP
Sbjct: 66  CDYGSCSWDHASILNLDLNNVIL-----QNAIKAFAPLKIRIGGTLQDIVIYETPDSKQP 125

Query: 129 CISFKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFASDGSAVGAW 188
           C+ F +N+  LFG+TQGCLP+ RWD+LN FF+K G K+IFGLNAL+GR   S+G A+GAW
Sbjct: 126 CLPFTKNSSILFGYTQGCLPMRRWDELNAFFRKTGTKVIFGLNALSGRSIKSNGEAIGAW 185

Query: 189 NYTNAESF---------------------------------------------------- 248
           NYTNAESF                                                    
Sbjct: 186 NYTNAESFIRFTAENNYTIDGWELGNELCGSGVGARVGANQYAIDTINLRNIVNRVYKNV 245

Query: 249 ------------------------------------------------------------ 308
                                                                       
Sbjct: 246 SPMPLVIGPGGFFEVDWFTEYLNKAENSLNATTRHIYDLGPGVDEHLIEKILNPSYLDQE 305

Query: 309 ------------------------------------------------------------ 319
                                                                       
Sbjct: 306 AKSFRSLKNIIKNSSTKAVAWVGESGGAYNSGRNLVSNAFVYSFWYLDQLGMASLYDTKT 365

BLAST of Cp4.1LG11g10490 vs. ExPASy Swiss-Prot
Match: Q8L608 (Heparanase-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At5g61250 PE=2 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 5.4e-40
Identity = 76/163 (46.63%), Postives = 106/163 (65.03%), Query Frame = 0

Query: 40  TVLINGKSAIGGIDEDFVCATLDWWPPEKCDYGTCSWGQASLLNLLPLWVFVFISKEQRK 99
           T++I+G   I   DE+F+CATLDWWPPEKC+Y  C WG ASL+NL         S    K
Sbjct: 28  TLVIDGSRRIAETDENFICATLDWWPPEKCNYDQCPWGYASLINL------NLASPLLAK 87

Query: 100 HVE-FKPLKLRLGGTLQDKLIYDTEDHKQPCISFKRNTKELFGFTQGCLPLDRWDKLNDF 159
            ++ F+ L++R+GG+LQD++IYD  D K PC  FK+    LFGF++GCL + RWD++N F
Sbjct: 88  AIQAFRTLRIRIGGSLQDQVIYDVGDLKTPCTQFKKTDDGLFGFSEGCLYMKRWDEVNHF 147

Query: 160 FKKGGAKIIFGLNALNGRKFASDGSAVGAWNYTNAESFKGITL 202
           F   GA + FGLNAL+GR   +  +  G W++TN + F   T+
Sbjct: 148 FNATGAIVTFGLNALHGRNKLNGTAWGGDWDHTNTQDFMNYTV 184


HSP 2 Score: 74.3 bits (181), Expect = 2.8e-12
Identity = 44/125 (35.20%), Postives = 70/125 (56.00%), Query Frame = 0

Query: 198 GITLLLLNLDGNTTVHAAVAFNRTSGLHYKHRSYKNKQKVVRTSRPY----GREGEAFRE 257
           GIT+LL+NL  +TT   AV+      L  +    K+  + +++   +      +G   RE
Sbjct: 414 GITILLINLSKHTTFTVAVSNGVKVVLQAESMKRKSFLETIKSKVSWVGNKASDGYLNRE 473

Query: 258 EYHITAKDGNLHSQTVLLNGKILSVNSSGNIPSLQPKQVNSSEPIMVAPFSIVFVHIPNI 317
           EYH++ KDG+L S+ +LLNGK L   ++G+IP L+P +     P+ + P SI F+ +P  
Sbjct: 474 EYHLSPKDGDLRSKIMLLNGKPLVPTATGDIPKLEPVRHGVKSPVYINPLSISFIVLPTF 533

Query: 318 VLPAC 319
             PAC
Sbjct: 534 DAPAC 538

BLAST of Cp4.1LG11g10490 vs. ExPASy Swiss-Prot
Match: Q9FF10 (Heparanase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g07830 PE=2 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 3.0e-35
Identity = 80/202 (39.60%), Postives = 114/202 (56.44%), Query Frame = 0

Query: 3   MGFRFWVLGFCFWVCFFFSCSGSVNSKGEEGDGFLSGTVLINGKSAIGGIDEDFVCATLD 62
           MGFR  V      +  F  C   V  K          +++I G   +   DE+FVCATLD
Sbjct: 1   MGFRVCV------IVVFLGCLLLVPEK-TMAQEMKRASIVIQGARRVCETDENFVCATLD 60

Query: 63  WWPPEKCDYGTCSWGQASLLNL---LPLWVFVFISKEQRKHVEFKPLKLRLGGTLQDKLI 122
           WWP +KC+Y  C WG +S++N+    PL     ++K  +    FKPL++R+GG+LQD++I
Sbjct: 61  WWPHDKCNYDQCPWGYSSVINMDLTRPL-----LTKAIK---AFKPLRIRIGGSLQDQVI 120

Query: 123 YDTEDHKQPCISFKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFA 182
           YD  + K PC  F++    LFGF++GCL + RWD+LN F    GA + FGLNAL GR   
Sbjct: 121 YDVGNLKTPCRPFQKMNSGLFGFSKGCLHMKRWDELNSFLTATGAVVTFGLNALRGRHKL 180

Query: 183 SDGSAVGAWNYTNAESFKGITL 202
              +  GAW++ N + F   T+
Sbjct: 181 RGKAWGGAWDHINTQDFLNYTV 187


HSP 2 Score: 71.2 bits (173), Expect = 2.3e-11
Identity = 45/127 (35.43%), Postives = 71/127 (55.91%), Query Frame = 0

Query: 198 GITLLLLNLDGNTTVHAAVAFNRTSGLHYKHRSYKNKQKVVRTSRPY------GREGEAF 257
           G+TLLL+NL   +    +V+      L+ + R  K K  +    RP+        +G   
Sbjct: 418 GVTLLLINLSNQSDFTVSVSNGINVVLNAESR--KKKSLLDTLKRPFSWIGSKASDGYLN 477

Query: 258 REEYHITAKDGNLHSQTVLLNGKILSVNSSGNIPSLQPKQVNSSEPIMVAPFSIVFVHIP 317
           REEYH+T ++G L S+T++LNGK L   ++G+IPSL+P   + + P+ V P S+ F+ +P
Sbjct: 478 REEYHLTPENGVLRSKTMVLNGKSLKPTATGDIPSLEPVLRSVNSPLNVLPLSMSFIVLP 537

Query: 318 NIVLPAC 319
           N    AC
Sbjct: 538 NFDASAC 542

BLAST of Cp4.1LG11g10490 vs. ExPASy Swiss-Prot
Match: Q9LRC8 (Baicalin-beta-D-glucuronidase OS=Scutellaria baicalensis OX=65409 GN=SGUS PE=1 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 3.7e-33
Identity = 86/208 (41.35%), Postives = 117/208 (56.25%), Query Frame = 0

Query: 3   MGFRFWVLGFCFWVCF--FFSCSGSVNSKGEEGDGFLSGTVLINGKSAIGGIDEDFVCAT 62
           MGF+ W  G C  +CF   F C G +   GEE       T++   ++ +   DE++VCAT
Sbjct: 1   MGFQVWQKGLCV-LCFSLIFIC-GVI---GEE------TTIVKIEENPVAQTDENYVCAT 60

Query: 63  LDWWPPEKCDYGTCSWGQASLLNLLPLWVFVFISKEQRKHV-EFKPLKLRLGGTLQDKLI 122
           LD WPP KC+YG C WG++S LNL         +   R  V EF PLKLR GGTLQD+L+
Sbjct: 61  LDLWPPTKCNYGNCPWGKSSFLNL------DLNNNIIRNAVKEFAPLKLRFGGTLQDRLV 120

Query: 123 YDTEDHKQPCIS-FKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKF 182
           Y T    +PC S F  NT  +  F+  CL LDRWD++N F  + G++ +FGLNAL G+  
Sbjct: 121 YQT-SRDEPCDSTFYNNTNLILDFSHACLSLDRWDEINQFILETGSEAVFGLNALRGKTV 180

Query: 183 ASDG------------SAVGAWNYTNAE 195
              G            +AVG W+Y+N++
Sbjct: 181 EIKGIIKDGQYLGETTTAVGEWDYSNSK 190


HSP 2 Score: 63.5 bits (153), Expect = 4.9e-09
Identity = 29/60 (48.33%), Postives = 45/60 (75.00%), Query Frame = 0

Query: 252 REEYHITAKDGNLHSQTVLLNGKILSVNSSGNIPSLQPKQVNSSEPIMVAPFSIVFVHIP 311
           REEYH+T  + NL S+ V LNG++L ++ SG IP+L P + ++S+ + VAP+S +FVH+P
Sbjct: 454 REEYHLTPVNNNLQSRLVKLNGELLHLDPSGVIPALNPVEKDNSKQLEVAPYSFMFVHLP 513

BLAST of Cp4.1LG11g10490 vs. NCBI nr
Match: XP_023546141.1 (heparanase-like protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 535 bits (1379), Expect = 1.57e-186
Identity = 307/555 (55.32%), Postives = 311/555 (56.04%), Query Frame = 0

Query: 1   MEMGFRFWVLGFCFWVCFFFSCSGSVNSKGEEGDGFLSGTVLINGKSAIGGIDEDFVCAT 60
           MEMGFRFWVLGFCFWVCFFFSCSGSVNSKGEEGDGFLSGTVLINGKSAIGGIDEDFVCAT
Sbjct: 1   MEMGFRFWVLGFCFWVCFFFSCSGSVNSKGEEGDGFLSGTVLINGKSAIGGIDEDFVCAT 60

Query: 61  LDWWPPEKCDYGTCSWGQASLLNLLPLWVFVFISKEQRKHVEFKPLKLRLGGTLQDKLIY 120
           LDWWPPEKCDYGTCSWGQASLLNL  L   + ++  +    EFKPLKLRLGGTLQDKLIY
Sbjct: 61  LDWWPPEKCDYGTCSWGQASLLNL-DLGSNILLNAIK----EFKPLKLRLGGTLQDKLIY 120

Query: 121 DTEDHKQPCISFKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFAS 180
           DTEDHKQPCISFKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFAS
Sbjct: 121 DTEDHKQPCISFKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFAS 180

Query: 181 DGSAVGAWNYTNAESF-------------------------------------------- 240
           DGSAVGAWNYTNAESF                                            
Sbjct: 181 DGSAVGAWNYTNAESFVRYTVKKNYTIHGWEFGNELCGSGVGTRISAEQYASDTIVFQKM 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 VQSIYNGVQTKPLTIAPGGFFDENWFKEFVDKTTPTLDAVTHHIYNLGAGVDEHLVERIL 300

Query: 301 ------------------------------------------------------------ 323
                                                                       
Sbjct: 301 NPSYLNGMVDTFYKLHEVLRNSRSSAKAWVGEAGGAYSSGHNLVSNAFVSSFWYLDQLGM 360

BLAST of Cp4.1LG11g10490 vs. NCBI nr
Match: XP_022999298.1 (heparanase-like protein 3 [Cucurbita maxima])

HSP 1 Score: 514 bits (1324), Expect = 3.28e-178
Identity = 298/553 (53.89%), Postives = 303/553 (54.79%), Query Frame = 0

Query: 3   MGFRFWVLGFCFWVCFFFSCSGSVNSKGEEGDGFLSGTVLINGKSAIGGIDEDFVCATLD 62
           M FRFWVLGFCFWVCFFFSCSGSVNSKGEEGDGFLSG VLI+GKSAIGGIDEDFVCATLD
Sbjct: 1   MDFRFWVLGFCFWVCFFFSCSGSVNSKGEEGDGFLSGMVLIDGKSAIGGIDEDFVCATLD 60

Query: 63  WWPPEKCDYGTCSWGQASLLNLLPLWVFVFISKEQRKHVEFKPLKLRLGGTLQDKLIYDT 122
           WWP EKCDYGTCSWGQASLLNL  L   + ++  +    EF PLKLRLGGTLQDKLIYDT
Sbjct: 61  WWPLEKCDYGTCSWGQASLLNL-DLGSNILLNAIK----EFNPLKLRLGGTLQDKLIYDT 120

Query: 123 EDHKQPCISFKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFASDG 182
           EDHKQPCISFKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFASDG
Sbjct: 121 EDHKQPCISFKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFASDG 180

Query: 183 SAVGAWNYTNAESF---------------------------------------------- 242
           SAVGAWNYTNAESF                                              
Sbjct: 181 SAVGAWNYTNAESFIRYTVKKNYTIHGWEFGNELCGSGVGTRISAEQYASDTIVFQKMVQ 240

Query: 243 ------------------------------------------------------------ 302
                                                                       
Sbjct: 241 SIYNGVQTKPLTIAPGGFFDENWFKEFVDKTTPTLDAVTHHIYNLGAGVDNHLVERILNP 300

Query: 303 ------------------------------------------------------------ 323
                                                                       
Sbjct: 301 SYLNGMVDTFYKLHEVLRNSQSSAKAWVGEAGGAYSSGHNLVSNAFVSSFWYLDQLGMAA 360

BLAST of Cp4.1LG11g10490 vs. NCBI nr
Match: XP_022946152.1 (heparanase-like protein 3 [Cucurbita moschata])

HSP 1 Score: 513 bits (1322), Expect = 7.05e-178
Identity = 295/555 (53.15%), Postives = 304/555 (54.77%), Query Frame = 0

Query: 1   MEMGFRFWVLGFCFWVCFFFSCSGSVNSKGEEGDGFLSGTVLINGKSAIGGIDEDFVCAT 60
           MEMGFRFWVLGFCFWVCFFFSCSGSVNS GEEGDGFLSGTV I+GKSAIGGIDEDF+CAT
Sbjct: 1   MEMGFRFWVLGFCFWVCFFFSCSGSVNSNGEEGDGFLSGTVSIDGKSAIGGIDEDFICAT 60

Query: 61  LDWWPPEKCDYGTCSWGQASLLNLLPLWVFVFISKEQRKHVEFKPLKLRLGGTLQDKLIY 120
           LDWWPPEKCDYGTCSWGQASLLNL  L   + ++  +    EFKPLKLRLGGTLQDKLIY
Sbjct: 61  LDWWPPEKCDYGTCSWGQASLLNL-DLGSNILLNAIK----EFKPLKLRLGGTLQDKLIY 120

Query: 121 DTEDHKQPCISFKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFAS 180
           DTEDHKQPCISFKR+TKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFAS
Sbjct: 121 DTEDHKQPCISFKRDTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFAS 180

Query: 181 DGSAVGAWNYTNAESF-------------------------------------------- 240
           +GSAVGAWNY NAESF                                            
Sbjct: 181 NGSAVGAWNYMNAESFVRYTVKKNYIIHGWEFGNELCGSGVGTRISAEQYASDTIVFQKM 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 VQSIYNGVQTKPLTIAPGGFFDENWFKEFVDKTTPTLDAVTHHIYNLGAGVDKHLVERIL 300

Query: 301 ------------------------------------------------------------ 323
                                                                       
Sbjct: 301 NPSYLNGMVETFYKLHEVLRNSRSSAKAWVGEAGGAYSSGHNLVSNAFVSSFWYLDQLGM 360

BLAST of Cp4.1LG11g10490 vs. NCBI nr
Match: KAG7030357.1 (Heparanase-like protein 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 511 bits (1317), Expect = 3.78e-177
Identity = 295/553 (53.35%), Postives = 303/553 (54.79%), Query Frame = 0

Query: 3   MGFRFWVLGFCFWVCFFFSCSGSVNSKGEEGDGFLSGTVLINGKSAIGGIDEDFVCATLD 62
           MGFRFWVLGFCFWVCFFFSCSGSVNS GEEGDGFLSGTV I+GKSAIGGI+EDF+CATLD
Sbjct: 1   MGFRFWVLGFCFWVCFFFSCSGSVNSNGEEGDGFLSGTVSIDGKSAIGGINEDFICATLD 60

Query: 63  WWPPEKCDYGTCSWGQASLLNLLPLWVFVFISKEQRKHVEFKPLKLRLGGTLQDKLIYDT 122
           WWPPEKCDYGTCSWGQASLLNL  L   V ++  +    EFKPLKLRLGGTLQDKLIYDT
Sbjct: 61  WWPPEKCDYGTCSWGQASLLNL-DLGSNVLLNAIK----EFKPLKLRLGGTLQDKLIYDT 120

Query: 123 EDHKQPCISFKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFASDG 182
           EDHKQPCISFKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFAS+G
Sbjct: 121 EDHKQPCISFKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFASNG 180

Query: 183 SAVGAWNYTNAESF---------------------------------------------- 242
           SAVGAWNYTNAESF                                              
Sbjct: 181 SAVGAWNYTNAESFVRYTIKKNYTIHGWEFGNELCGSGVGTRISAEQYASDTIVFQKMVQ 240

Query: 243 ------------------------------------------------------------ 302
                                                                       
Sbjct: 241 SIYNGVQTKPLTIAPGGFFDENWFKEFVDKTTPTLDAVTHHIYNLGAGVDKHLVERILNP 300

Query: 303 ------------------------------------------------------------ 323
                                                                       
Sbjct: 301 SYLNGMVETFYKLHEVLRNSRSSAKAWVGEAGGAYSSGHNLVSNAFVSSFWYLDQLGMAA 360

BLAST of Cp4.1LG11g10490 vs. NCBI nr
Match: KAG6599370.1 (Heparanase-like protein 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 471 bits (1212), Expect = 4.19e-159
Identity = 277/535 (51.78%), Postives = 285/535 (53.27%), Query Frame = 0

Query: 21  SCSGSVNSKGEEGDGFLSGTVLINGKSAIGGIDEDFVCATLDWWPPEKCDYGTCSWGQAS 80
           +CSGSVNS GEEGDGFLSGTV I+GKSAIGGIDEDF+CATLDWWPPEKCDYGTCSWGQAS
Sbjct: 174 NCSGSVNSNGEEGDGFLSGTVSIDGKSAIGGIDEDFICATLDWWPPEKCDYGTCSWGQAS 233

Query: 81  LLNLLPLWVFVFISKEQRKHVEFKPLKLRLGGTLQDKLIYDTEDHKQPCISFKRNTKELF 140
           LLNL  L   V ++  +    EFKPLKLRLGGTLQDKLIYDTEDHKQPCISFKR+TKELF
Sbjct: 234 LLNL-DLGSNVLLNAIK----EFKPLKLRLGGTLQDKLIYDTEDHKQPCISFKRDTKELF 293

Query: 141 GFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFASDGSAVGAWNYTNAESF---- 200
           GFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFASDGSAVGAWNYTNAESF    
Sbjct: 294 GFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFASDGSAVGAWNYTNAESFVRYT 353

Query: 201 ------------------------------------------------------------ 260
                                                                       
Sbjct: 354 VKKNYTIHGWEFGNELCGSGVGTRISAEQYASDTIVFQKMVQSIYNGVQTKPLTIAPGGF 413

Query: 261 ------------------------------------------------------------ 320
                                                                       
Sbjct: 414 FDENWFKEFVDKTTPTLDAVTHHIYNLGAGVDKHLVERILNPSYLNGMVETFYKLHEVLR 473

Query: 321 ------------------------------------------------------------ 323
                                                                       
Sbjct: 474 NSRSSAKAWVGEAGGAYSSGHNLVSNAFVSSFWYLDQLGMAATFDTKTYCRQTLIGGNYG 533

BLAST of Cp4.1LG11g10490 vs. ExPASy TrEMBL
Match: A0A6J1KJ91 (heparanase-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111493713 PE=3 SV=1)

HSP 1 Score: 514 bits (1324), Expect = 1.59e-178
Identity = 298/553 (53.89%), Postives = 303/553 (54.79%), Query Frame = 0

Query: 3   MGFRFWVLGFCFWVCFFFSCSGSVNSKGEEGDGFLSGTVLINGKSAIGGIDEDFVCATLD 62
           M FRFWVLGFCFWVCFFFSCSGSVNSKGEEGDGFLSG VLI+GKSAIGGIDEDFVCATLD
Sbjct: 1   MDFRFWVLGFCFWVCFFFSCSGSVNSKGEEGDGFLSGMVLIDGKSAIGGIDEDFVCATLD 60

Query: 63  WWPPEKCDYGTCSWGQASLLNLLPLWVFVFISKEQRKHVEFKPLKLRLGGTLQDKLIYDT 122
           WWP EKCDYGTCSWGQASLLNL  L   + ++  +    EF PLKLRLGGTLQDKLIYDT
Sbjct: 61  WWPLEKCDYGTCSWGQASLLNL-DLGSNILLNAIK----EFNPLKLRLGGTLQDKLIYDT 120

Query: 123 EDHKQPCISFKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFASDG 182
           EDHKQPCISFKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFASDG
Sbjct: 121 EDHKQPCISFKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFASDG 180

Query: 183 SAVGAWNYTNAESF---------------------------------------------- 242
           SAVGAWNYTNAESF                                              
Sbjct: 181 SAVGAWNYTNAESFIRYTVKKNYTIHGWEFGNELCGSGVGTRISAEQYASDTIVFQKMVQ 240

Query: 243 ------------------------------------------------------------ 302
                                                                       
Sbjct: 241 SIYNGVQTKPLTIAPGGFFDENWFKEFVDKTTPTLDAVTHHIYNLGAGVDNHLVERILNP 300

Query: 303 ------------------------------------------------------------ 323
                                                                       
Sbjct: 301 SYLNGMVDTFYKLHEVLRNSQSSAKAWVGEAGGAYSSGHNLVSNAFVSSFWYLDQLGMAA 360

BLAST of Cp4.1LG11g10490 vs. ExPASy TrEMBL
Match: A0A6J1G2Y8 (heparanase-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111450316 PE=3 SV=1)

HSP 1 Score: 513 bits (1322), Expect = 3.41e-178
Identity = 295/555 (53.15%), Postives = 304/555 (54.77%), Query Frame = 0

Query: 1   MEMGFRFWVLGFCFWVCFFFSCSGSVNSKGEEGDGFLSGTVLINGKSAIGGIDEDFVCAT 60
           MEMGFRFWVLGFCFWVCFFFSCSGSVNS GEEGDGFLSGTV I+GKSAIGGIDEDF+CAT
Sbjct: 1   MEMGFRFWVLGFCFWVCFFFSCSGSVNSNGEEGDGFLSGTVSIDGKSAIGGIDEDFICAT 60

Query: 61  LDWWPPEKCDYGTCSWGQASLLNLLPLWVFVFISKEQRKHVEFKPLKLRLGGTLQDKLIY 120
           LDWWPPEKCDYGTCSWGQASLLNL  L   + ++  +    EFKPLKLRLGGTLQDKLIY
Sbjct: 61  LDWWPPEKCDYGTCSWGQASLLNL-DLGSNILLNAIK----EFKPLKLRLGGTLQDKLIY 120

Query: 121 DTEDHKQPCISFKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFAS 180
           DTEDHKQPCISFKR+TKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFAS
Sbjct: 121 DTEDHKQPCISFKRDTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFAS 180

Query: 181 DGSAVGAWNYTNAESF-------------------------------------------- 240
           +GSAVGAWNY NAESF                                            
Sbjct: 181 NGSAVGAWNYMNAESFVRYTVKKNYIIHGWEFGNELCGSGVGTRISAEQYASDTIVFQKM 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 VQSIYNGVQTKPLTIAPGGFFDENWFKEFVDKTTPTLDAVTHHIYNLGAGVDKHLVERIL 300

Query: 301 ------------------------------------------------------------ 323
                                                                       
Sbjct: 301 NPSYLNGMVETFYKLHEVLRNSRSSAKAWVGEAGGAYSSGHNLVSNAFVSSFWYLDQLGM 360

BLAST of Cp4.1LG11g10490 vs. ExPASy TrEMBL
Match: A0A1S3C565 (LOW QUALITY PROTEIN: heparanase-like protein 3 OS=Cucumis melo OX=3656 GN=LOC103496979 PE=3 SV=1)

HSP 1 Score: 410 bits (1053), Expect = 1.31e-137
Identity = 248/548 (45.26%), Postives = 273/548 (49.82%), Query Frame = 0

Query: 3   MGFRFWVLGFCFWVCFFFSCSGSVNSKGEEGDGFLSGTVLINGKSAIGGIDEDFVCATLD 62
           MGFRFW+LGFCFWVCFFF C GSVNS+G     FL G V I+GKSAIG IDEDFVCATLD
Sbjct: 1   MGFRFWLLGFCFWVCFFFLCCGSVNSEG-----FLRGRVFIDGKSAIGRIDEDFVCATLD 60

Query: 63  WWPPEKCDYGTCSWGQASLLNLLPLWVFVFISKEQRKHVEFKPLKLRLGGTLQDKLIYDT 122
           WWPPEKCDYGTCSWGQASLLNL  L   + ++  +    EFKPLKLRLGGTLQDKLIYDT
Sbjct: 61  WWPPEKCDYGTCSWGQASLLNL-DLGNNILLNAVK----EFKPLKLRLGGTLQDKLIYDT 120

Query: 123 EDHKQPCISFKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFASDG 182
           EDH++PCI F RN  ELFGFTQGCLP +RWD+LNDFFKKGGAKIIFGLNALNGR+ A++G
Sbjct: 121 EDHREPCIYFSRNASELFGFTQGCLPTNRWDQLNDFFKKGGAKIIFGLNALNGREIAANG 180

Query: 183 SAVGAWNYTNAE------------------------------------------------ 242
            ++GAWNYTNAE                                                
Sbjct: 181 CSIGAWNYTNAEMLIRYTVKKNYTIHGWELGNELSGNGVGTRILAEQYASDTIVLQNMVQ 240

Query: 243 ------------------------------------------------------------ 302
                                                                       
Sbjct: 241 SIYKDIEPKPLIIAPGGFFDENWYKEFIDKTTPTLDVVTHHIYNLGPGVDEHLVERILDP 300

Query: 303 ------------------------------------------------SF---------- 318
                                                           SF          
Sbjct: 301 SYLDGMVDTFNKLHEILKNSPTSAKAWVGESGGAYNSGHNLVTNAFVFSFWYLDQLGMAA 360

BLAST of Cp4.1LG11g10490 vs. ExPASy TrEMBL
Match: A0A6J1HPC5 (heparanase-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111465495 PE=3 SV=1)

HSP 1 Score: 402 bits (1034), Expect = 1.18e-134
Identity = 242/550 (44.00%), Postives = 275/550 (50.00%), Query Frame = 0

Query: 3   MGFRFWVLGFCFWVCFFFSCSGSVNSKGE--EGDGFLSGTVLINGKSAIGGIDEDFVCAT 62
           MGFR W++GFCFWVCF F   GSVNSKG   E DGF+ G VLI+GKSAIGGIDEDFVCAT
Sbjct: 1   MGFRVWLVGFCFWVCFLFC--GSVNSKGLGLEEDGFVRGRVLIDGKSAIGGIDEDFVCAT 60

Query: 63  LDWWPPEKCDYGTCSWGQASLLNLLPLWVFVFISKEQRKHVEFKPLKLRLGGTLQDKLIY 122
           LDWWPPEKCDYGTCSWG+ASLLNL  L   V ++  +    EFKPLKLRLGGTLQDK+IY
Sbjct: 61  LDWWPPEKCDYGTCSWGRASLLNL-DLGNNVLLNAVK----EFKPLKLRLGGTLQDKIIY 120

Query: 123 DTEDHKQPCISFKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFAS 182
           +TEDH+Q C+   RNT ELFG++QGCLP  RWD+LN+FFKKGG K+IFGLNALNGR+ AS
Sbjct: 121 ETEDHQQSCVYLSRNTTELFGYSQGCLPTKRWDELNEFFKKGGVKVIFGLNALNGRQIAS 180

Query: 183 DGSAVGAWNYTNAESF-------------------------------------------- 242
           DGSAVGAW++TNAESF                                            
Sbjct: 181 DGSAVGAWDHTNAESFIRYTVKKNYTIHGWELGNELSGNGVGTRVTAEQYASDTIALQNM 240

Query: 243 ------------------------------------------------------------ 302
                                                                       
Sbjct: 241 VQSIYKDIEYKPLIISPGGFFDENWFKEFIDKTTQSLDVVTHHIYNLGSGVDEHLVERIL 300

Query: 303 ------------------------------------------------------------ 318
                                                                       
Sbjct: 301 DPSYLDGMADTFYKLHEILRNSPTSAKAWVGEAGGAYNSGHNLVTNAFVFSFWYLDQLGM 360

BLAST of Cp4.1LG11g10490 vs. ExPASy TrEMBL
Match: A0A6J1CML0 (heparanase-like protein 3 OS=Momordica charantia OX=3673 GN=LOC111013018 PE=3 SV=1)

HSP 1 Score: 397 bits (1020), Expect = 1.23e-132
Identity = 242/543 (44.57%), Postives = 267/543 (49.17%), Query Frame = 0

Query: 10  LGFCFWVCFFFSCSGSVNSKG-EEGDGFLSGTVLINGKSAIGGIDEDFVCATLDWWPPEK 69
           +GFCFWVCFFF C  SV+SKG EE DG + G VLI+GKSAI GIDEDFVCATLDWWPPEK
Sbjct: 1   MGFCFWVCFFF-CFASVSSKGVEEDDGCVRGRVLIDGKSAIAGIDEDFVCATLDWWPPEK 60

Query: 70  CDYGTCSWGQASLLNL-LPLWVFVFISKEQRKHVEFKPLKLRLGGTLQDKLIYDTEDHKQ 129
           CDYGTCSWG+ASLLNL L   + +   KE      FKPLKLRLGG+LQDKLIYDTEDH+Q
Sbjct: 61  CDYGTCSWGRASLLNLDLDNNILLNAVKE------FKPLKLRLGGSLQDKLIYDTEDHQQ 120

Query: 130 PCISFKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFASDGSAVGA 189
           PCISF RNT ELFGF+QGCLP  RWD+LNDFFKKGGAKIIFGLNAL GR+ ASDGSA+GA
Sbjct: 121 PCISFSRNTSELFGFSQGCLPTYRWDQLNDFFKKGGAKIIFGLNALTGRRIASDGSAIGA 180

Query: 190 WNYTNAESF--------------------------------------------------- 249
           WNYTNAESF                                                   
Sbjct: 181 WNYTNAESFIRYTVKKNYTIHGWELGNELSGNGVGTRISADQYASDTYALQNMVRSIYQG 240

Query: 250 ------------------------------------------------------------ 309
                                                                       
Sbjct: 241 IEPKPLIIAPGGFFDENWFKDFIDKTXQSADVITHHIYNLGPGVDEHLVERILDPSYLDG 300

Query: 310 ------------------------------------------------------------ 318
                                                                       
Sbjct: 301 MVDTFYRLHEILKSSPTSAKPWVGEAGGAYNSGHNLVTNAFVFSFWYLDQLGMSAAFDTK 360

BLAST of Cp4.1LG11g10490 vs. TAIR 10
Match: AT5G34940.2 (glucuronidase 3 )

HSP 1 Score: 254.6 bits (649), Expect = 1.1e-67
Identity = 175/544 (32.17%), Postives = 222/544 (40.81%), Query Frame = 0

Query: 9   VLGFCFWVCFFFSCSGSVNSKGEEGDGFLSGTVLINGKSAIGGIDEDFVCATLDWWPPEK 68
           +L    ++C F     +V+S  EE     +GTV + G++A+G IDEDF+CATLDWWPPEK
Sbjct: 6   ILAIVLFLCVFQFLDCTVSSAVEE-----NGTVFVYGRAAVGTIDEDFICATLDWWPPEK 65

Query: 69  CDYGTCSWGQASLLNLLPLWVFVFISKEQRKHVEFKPLKLRLGGTLQDKLIYDTEDHKQP 128
           CDYG+CSW  AS+LNL    V +     Q     F PLK+R+GGTLQD +IY+T D KQP
Sbjct: 66  CDYGSCSWDHASILNLDLNNVIL-----QNAIKAFAPLKIRIGGTLQDIVIYETPDSKQP 125

Query: 129 CISFKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFASDGSAVGAW 188
           C+ F +N+  LFG+TQGCLP+ RWD+LN FF+K G K+IFGLNAL+GR   S+G A+GAW
Sbjct: 126 CLPFTKNSSILFGYTQGCLPMRRWDELNAFFRKTGTKVIFGLNALSGRSIKSNGEAIGAW 185

Query: 189 NYTNAESF---------------------------------------------------- 248
           NYTNAESF                                                    
Sbjct: 186 NYTNAESFIRFTAENNYTIDGWELGNELCGSGVGARVGANQYAIDTINLRNIVNRVYKNV 245

Query: 249 ------------------------------------------------------------ 308
                                                                       
Sbjct: 246 SPMPLVIGPGGFFEVDWFTEYLNKAENSLNATTRHIYDLGPGVDEHLIEKILNPSYLDQE 305

Query: 309 ------------------------------------------------------------ 319
                                                                       
Sbjct: 306 AKSFRSLKNIIKNSSTKAVAWVGESGGAYNSGRNLVSNAFVYSFWYLDQLGMASLYDTKT 365

BLAST of Cp4.1LG11g10490 vs. TAIR 10
Match: AT5G34940.3 (glucuronidase 3 )

HSP 1 Score: 232.3 bits (591), Expect = 5.7e-61
Identity = 109/188 (57.98%), Postives = 139/188 (73.94%), Query Frame = 0

Query: 9   VLGFCFWVCFFFSCSGSVNSKGEEGDGFLSGTVLINGKSAIGGIDEDFVCATLDWWPPEK 68
           +L    ++C F     +V+S  EE     +GTV + G++A+G IDEDF+CATLDWWPPEK
Sbjct: 6   ILAIVLFLCVFQFLDCTVSSAVEE-----NGTVFVYGRAAVGTIDEDFICATLDWWPPEK 65

Query: 69  CDYGTCSWGQASLLNLLPLWVFVFISKEQRKHVEFKPLKLRLGGTLQDKLIYDTEDHKQP 128
           CDYG+CSW  AS+LN L L + V IS    K+  F PLK+R+GGTLQD +IY+T D KQP
Sbjct: 66  CDYGSCSWDHASILN-LDLTIVVPIS----KNTAFAPLKIRIGGTLQDIVIYETPDSKQP 125

Query: 129 CISFKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFASDGSAVGAW 188
           C+ F +N+  LFG+TQGCLP+ RWD+LN FF+K G K+IFGLNAL+GR   S+G A+GAW
Sbjct: 126 CLPFTKNSSILFGYTQGCLPMRRWDELNAFFRKTGTKVIFGLNALSGRSIKSNGEAIGAW 183

Query: 189 NYTNAESF 197
           NYTNAESF
Sbjct: 186 NYTNAESF 183

BLAST of Cp4.1LG11g10490 vs. TAIR 10
Match: AT5G61250.2 (glucuronidase 1 )

HSP 1 Score: 166.4 bits (420), Expect = 3.8e-41
Identity = 76/163 (46.63%), Postives = 106/163 (65.03%), Query Frame = 0

Query: 40  TVLINGKSAIGGIDEDFVCATLDWWPPEKCDYGTCSWGQASLLNLLPLWVFVFISKEQRK 99
           T++I+G   I   DE+F+CATLDWWPPEKC+Y  C WG ASL+NL         S    K
Sbjct: 28  TLVIDGSRRIAETDENFICATLDWWPPEKCNYDQCPWGYASLINL------NLASPLLAK 87

Query: 100 HVE-FKPLKLRLGGTLQDKLIYDTEDHKQPCISFKRNTKELFGFTQGCLPLDRWDKLNDF 159
            ++ F+ L++R+GG+LQD++IYD  D K PC  FK+    LFGF++GCL + RWD++N F
Sbjct: 88  AIQAFRTLRIRIGGSLQDQVIYDVGDLKTPCTQFKKTDDGLFGFSEGCLYMKRWDEVNHF 147

Query: 160 FKKGGAKIIFGLNALNGRKFASDGSAVGAWNYTNAESFKGITL 202
           F   GA + FGLNAL+GR   +  +  G W++TN + F   T+
Sbjct: 148 FNATGAIVTFGLNALHGRNKLNGTAWGGDWDHTNTQDFMNYTV 184


HSP 2 Score: 74.3 bits (181), Expect = 2.0e-13
Identity = 44/125 (35.20%), Postives = 70/125 (56.00%), Query Frame = 0

Query: 198 GITLLLLNLDGNTTVHAAVAFNRTSGLHYKHRSYKNKQKVVRTSRPY----GREGEAFRE 257
           GIT+LL+NL  +TT   AV+      L  +    K+  + +++   +      +G   RE
Sbjct: 414 GITILLINLSKHTTFTVAVSNGVKVVLQAESMKRKSFLETIKSKVSWVGNKASDGYLNRE 473

Query: 258 EYHITAKDGNLHSQTVLLNGKILSVNSSGNIPSLQPKQVNSSEPIMVAPFSIVFVHIPNI 317
           EYH++ KDG+L S+ +LLNGK L   ++G+IP L+P +     P+ + P SI F+ +P  
Sbjct: 474 EYHLSPKDGDLRSKIMLLNGKPLVPTATGDIPKLEPVRHGVKSPVYINPLSISFIVLPTF 533

Query: 318 VLPAC 319
             PAC
Sbjct: 534 DAPAC 538

BLAST of Cp4.1LG11g10490 vs. TAIR 10
Match: AT5G61250.1 (glucuronidase 1 )

HSP 1 Score: 166.4 bits (420), Expect = 3.8e-41
Identity = 76/163 (46.63%), Postives = 106/163 (65.03%), Query Frame = 0

Query: 40  TVLINGKSAIGGIDEDFVCATLDWWPPEKCDYGTCSWGQASLLNLLPLWVFVFISKEQRK 99
           T++I+G   I   DE+F+CATLDWWPPEKC+Y  C WG ASL+NL         S    K
Sbjct: 28  TLVIDGSRRIAETDENFICATLDWWPPEKCNYDQCPWGYASLINL------NLASPLLAK 87

Query: 100 HVE-FKPLKLRLGGTLQDKLIYDTEDHKQPCISFKRNTKELFGFTQGCLPLDRWDKLNDF 159
            ++ F+ L++R+GG+LQD++IYD  D K PC  FK+    LFGF++GCL + RWD++N F
Sbjct: 88  AIQAFRTLRIRIGGSLQDQVIYDVGDLKTPCTQFKKTDDGLFGFSEGCLYMKRWDEVNHF 147

Query: 160 FKKGGAKIIFGLNALNGRKFASDGSAVGAWNYTNAESFKGITL 202
           F   GA + FGLNAL+GR   +  +  G W++TN + F   T+
Sbjct: 148 FNATGAIVTFGLNALHGRNKLNGTAWGGDWDHTNTQDFMNYTV 184


HSP 2 Score: 74.3 bits (181), Expect = 2.0e-13
Identity = 44/125 (35.20%), Postives = 70/125 (56.00%), Query Frame = 0

Query: 198 GITLLLLNLDGNTTVHAAVAFNRTSGLHYKHRSYKNKQKVVRTSRPY----GREGEAFRE 257
           GIT+LL+NL  +TT   AV+      L  +    K+  + +++   +      +G   RE
Sbjct: 414 GITILLINLSKHTTFTVAVSNGVKVVLQAESMKRKSFLETIKSKVSWVGNKASDGYLNRE 473

Query: 258 EYHITAKDGNLHSQTVLLNGKILSVNSSGNIPSLQPKQVNSSEPIMVAPFSIVFVHIPNI 317
           EYH++ KDG+L S+ +LLNGK L   ++G+IP L+P +     P+ + P SI F+ +P  
Sbjct: 474 EYHLSPKDGDLRSKIMLLNGKPLVPTATGDIPKLEPVRHGVKSPVYINPLSISFIVLPTF 533

Query: 318 VLPAC 319
             PAC
Sbjct: 534 DAPAC 538

BLAST of Cp4.1LG11g10490 vs. TAIR 10
Match: AT5G07830.1 (glucuronidase 2 )

HSP 1 Score: 150.6 bits (379), Expect = 2.2e-36
Identity = 80/202 (39.60%), Postives = 114/202 (56.44%), Query Frame = 0

Query: 3   MGFRFWVLGFCFWVCFFFSCSGSVNSKGEEGDGFLSGTVLINGKSAIGGIDEDFVCATLD 62
           MGFR  V      +  F  C   V  K          +++I G   +   DE+FVCATLD
Sbjct: 1   MGFRVCV------IVVFLGCLLLVPEK-TMAQEMKRASIVIQGARRVCETDENFVCATLD 60

Query: 63  WWPPEKCDYGTCSWGQASLLNL---LPLWVFVFISKEQRKHVEFKPLKLRLGGTLQDKLI 122
           WWP +KC+Y  C WG +S++N+    PL     ++K  +    FKPL++R+GG+LQD++I
Sbjct: 61  WWPHDKCNYDQCPWGYSSVINMDLTRPL-----LTKAIK---AFKPLRIRIGGSLQDQVI 120

Query: 123 YDTEDHKQPCISFKRNTKELFGFTQGCLPLDRWDKLNDFFKKGGAKIIFGLNALNGRKFA 182
           YD  + K PC  F++    LFGF++GCL + RWD+LN F    GA + FGLNAL GR   
Sbjct: 121 YDVGNLKTPCRPFQKMNSGLFGFSKGCLHMKRWDELNSFLTATGAVVTFGLNALRGRHKL 180

Query: 183 SDGSAVGAWNYTNAESFKGITL 202
              +  GAW++ N + F   T+
Sbjct: 181 RGKAWGGAWDHINTQDFLNYTV 187


HSP 2 Score: 71.2 bits (173), Expect = 1.7e-12
Identity = 45/127 (35.43%), Postives = 71/127 (55.91%), Query Frame = 0

Query: 198 GITLLLLNLDGNTTVHAAVAFNRTSGLHYKHRSYKNKQKVVRTSRPY------GREGEAF 257
           G+TLLL+NL   +    +V+      L+ + R  K K  +    RP+        +G   
Sbjct: 418 GVTLLLINLSNQSDFTVSVSNGINVVLNAESR--KKKSLLDTLKRPFSWIGSKASDGYLN 477

Query: 258 REEYHITAKDGNLHSQTVLLNGKILSVNSSGNIPSLQPKQVNSSEPIMVAPFSIVFVHIP 317
           REEYH+T ++G L S+T++LNGK L   ++G+IPSL+P   + + P+ V P S+ F+ +P
Sbjct: 478 REEYHLTPENGVLRSKTMVLNGKSLKPTATGDIPSLEPVLRSVNSPLNVLPLSMSFIVLP 537

Query: 318 NIVLPAC 319
           N    AC
Sbjct: 538 NFDASAC 542

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FZP11.5e-6632.17Heparanase-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g34940 PE=2 SV=2[more]
Q8L6085.4e-4046.63Heparanase-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At5g61250 PE=2 SV=1[more]
Q9FF103.0e-3539.60Heparanase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g07830 PE=2 SV=1[more]
Q9LRC83.7e-3341.35Baicalin-beta-D-glucuronidase OS=Scutellaria baicalensis OX=65409 GN=SGUS PE=1 S... [more]
Match NameE-valueIdentityDescription
XP_023546141.11.57e-18655.32heparanase-like protein 3 [Cucurbita pepo subsp. pepo][more]
XP_022999298.13.28e-17853.89heparanase-like protein 3 [Cucurbita maxima][more]
XP_022946152.17.05e-17853.15heparanase-like protein 3 [Cucurbita moschata][more]
KAG7030357.13.78e-17753.35Heparanase-like protein 3, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6599370.14.19e-15951.78Heparanase-like protein 3, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1KJ911.59e-17853.89heparanase-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111493713 PE=3 SV=1[more]
A0A6J1G2Y83.41e-17853.15heparanase-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111450316 PE=3 SV=... [more]
A0A1S3C5651.31e-13745.26LOW QUALITY PROTEIN: heparanase-like protein 3 OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1HPC51.18e-13444.00heparanase-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111465495 PE=3 SV=1[more]
A0A6J1CML01.23e-13244.57heparanase-like protein 3 OS=Momordica charantia OX=3673 GN=LOC111013018 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT5G34940.21.1e-6732.17glucuronidase 3 [more]
AT5G34940.35.7e-6157.98glucuronidase 3 [more]
AT5G61250.23.8e-4146.63glucuronidase 1 [more]
AT5G61250.13.8e-4146.63glucuronidase 1 [more]
AT5G07830.12.2e-3639.60glucuronidase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005199Glycoside hydrolase, family 79PFAMPF03662Glyco_hydro_79ncoord: 39..197
e-value: 3.1E-62
score: 210.3
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 94..212
e-value: 4.6E-14
score: 54.5
NoneNo IPR availablePANTHERPTHR14363HEPARANASE-RELATEDcoord: 8..195
coord: 197..318
NoneNo IPR availablePANTHERPTHR14363:SF34HEPARANASE-LIKE PROTEIN 3coord: 8..195
coord: 197..318
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..22
score: 5.0
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 52..193

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG11g10490.1Cp4.1LG11g10490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0016798 hydrolase activity, acting on glycosyl bonds