Cp4.1LG11g10480 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG11g10480
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionUbiquitin carboxyl-terminal hydrolase
LocationCp4.1LG11: 8768403 .. 8772341 (+)
RNA-Seq ExpressionCp4.1LG11g10480
SyntenyCp4.1LG11g10480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCTTCTCACCTTCTTACCCTCCTTCCCCATCGCTTTCACCTCCCCTCCCATGTCTTCCAATTTCCACCACCACCATCGTCACCACCCAAATCTTCCTTTCAACTCTCAGATCTTCCCCCCTTCTTGTCCCCACCTCGCCGACTTCCGTTCTCGAAATGGTTCCAAGTCCTTCCGCCTTCTACAAGACTGTCTTCGCGTCAAGCCCCCCGGTGGCCGCGCCGCCATCCGTCGAGACCCCAACGAGGTTCCCCGATGTGGTTCCTGCGGTGATTCCGCTCGTCCAAGGCTATATGCTTGTGTGACCTGCGCCGCCGTGTCCTGTCACGCGCCGCCCTCCCATTCCCATGCCGCGGCTCACGCCGAATCAATGCCGCCAGGTCATGAGATCGCTGTCGACGTCGATCGTGCCGAGCTTTTTTGCTGTGCTTGCCGCGACCAGGTTTATGATCGCGACTTTGACGCTGCAGTGGTGTTGGCTCAAACGACGGCGTCTACTCTTGGCGGGAATGCGGTCTCTTCCAGTGGAGCACCGCCGTCGCAGCCTCTGCTCCCGCCGGAGAATCTGAGGAAGCGGCGGAGGGTTGATTATCAGCCGTGGGTACCGGATCTGAGGGAGAGGGTTTTAGTGGGTGCTAATTCGAGTCCACTTGATTGTGCGAATTCCAATCCGGGTTCTGAATCTGAGTTGCCCTGGGGGTTGCGGGGATTGAACAATTTGGGGAATACGTGTTTTATGAACTCGGTCTTGCAAGCATTGCTGCATACTCCGCCGTTGAGGAATTTTTTCTTGAGTGATCGTCACAATCGCTATTTTTGTCAGAGAAATGTCAATGTGGGCAATAAAAATTCGAATGCCAACGATGGAAATGGCAGTAAAAATTCGCGCAGCTGTCTGGCTTGTGATCTGGATTCTATATTTTCTGCTGTCTTCTCCGGTGATCGGATCCCTTACAGCCCAGCAAAATTTTTATTGAGGTTTCAATCTGATGCATTTCTTGATCGTGATTTTCTCTTTCTTAGAATGTTTGATTCTTCAATGTTCGATTAGTTTTCTTTACTTTCGTTGTAAAGATGGGGTTCTTTTTTTCTTTCCAATTCACTCAATTGTTGTCTCATTGCAGCTGGTGGCAACATGCTGCAAACCTCGCAAGTTATGAACAACAAGATGCACACGAATTTTTCATCTCTATTCTCGAAGGCATCCACGAAAAGGTAGACAAAGATCGGCGAAAACCTTACAGTCAAGGTAAATTCCTGCTTTTCAATGTTCTGTTTGCTTTAAGATCCTTTTCACACAATGTACATGTTGTATTTACTTTTTCTCCCCCCCTCTCTCTCCTTTTGTTATTTCCTGTTTTCTTTTTTGCACTGGTTGCTGTTTGAAAGTCAAAGAACAGAATCATTTTCAAGTTTAGTTTGTTGCAAAATATGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNCTTATGATACTTTGTTCTTGTAAATACCAAATTATGTGCGGAACCTTGATGACGGCCCTGTATCGGCCTCCCGGGCATGGATTTTATGTATTCTTCATTGTTACATTTCCCTCCGTGATCTAGAACATGTTTGGAAGTGATTTTGAAATAGTCAGGATCATTTTTGTTATGTTCAAATATGCTTTTATTCATTTAAAGTTGATTTCGCTGGCATGAAATTATGTTTAGAAGTGAAAAATCGAGTATTTAATTGATTTCGAAGAATCAAATACATGTTTTTAGAGTGATTTTGAACATGAGAAAGCAATTTCAACGATCTTAGAGTCACTCCCAAACAAGCCTTTAGGCACTTCTCGAACACTTTTTTTATTGCACGAAATGCTCCTCGTGAAGTTGAAATGTTATGTATCCTTTCTAATACTATCATCTAGACGTGTGATTCTCTGTTACTGAGTTGTGAGCTCCATACTCTTGGCTTTTTACCATCAATGAAATCCTACTGATTTTGGTGCCGCAACACTTGTTTTGAAAACTACATCTAACCAAAGACTAATTGTCCCCATCCATTGACCCTTCCTGGCTTCGCCAGTTCTGCCAGGCATTCAGATAGGTGAATTGAGGCTGGAAAAGTTACTAATTGTATCGGCTGTCCATGATAAGGCTTGTCAGTTACTCTGCATCATTAAATAGCTTGGTTGTACATCTCCAACTGACTTCTTGGATAAGATGTCTCATTTGAATGGTGGGAAAATCCTTAGCTGCTTAAGTGGGTATTTTTATATTTTCACTCGAAAAGCCCGGTCAACAGTCGATGGATAACCCAAAGGCATATCATGTGAATGAAATTTGCTGCATTGCATATAAGAAAGACGATCAGCTTAAACTGATTTTTAATGATCACCTTATTTTATGTATATCAGCGTGTGTTTTTTCCCAAGCCAAGATAATTCATAGTGATGTGTATAAAGTTGGGAAGTAACCGTAAAATTTAACTGCAGGGTTTGAGTCTAGGAATGTTGCTATTGCTCTAGGAGTTGACAAGTGCTCAAAGATTTAGGGCTAATTTCCAAACTGAACCTGCTGGCTTGATGGTTTTTTCTACTTCTCACTTGTAAGTCAACTTTTTCTCAACCAACTTGGTTATGAGTTACTAGTAGGATCAACCACCTCACGTTTTTCTCACGCTAAAAATCCCTTTGTTCGAGTCCAATTTGCATTGTTGAGCTGAACACATTGACAATTATGAACCCATTCATGAGGAAAGGTCAGACCAGGTTTGTTAAGGATCACTGGTTCCTGGAAAATCTCCTAAACAATCCTCCATTAATGTCAAAGTTTCCAATATGAATAAAGATATTAATTATAAGCATAACTCGCTCGAGAAACGTATTGACGAATTCGAATAAATTTTGAGCTTCTTTTATCATGGTTTTTTAGAAAAATCATTTAGATTGATATTTGCATTGGCATCGTCATAAAACTAAGGCTCCGTACACTTCATTCATGTTAGATGGCTGTGGTTTATGGAGTTAAGCTTACTTGAAATGAATTTTTATGCCGCTGTCTTCTAGATTTTGTCTTAGCGCAGCATTTAACTATTAAGTTCTGATTTTGGTGTATGAGAATAATTCTGATTTTAGAATATTATTTCATTATAGTATTGAATGACTTATCCCTGTCTCTTTTCCAGTGCAGGCAATGGAGACTGTTGTATTGCTCATAGAGTATTTTCTGGCATACTGCGATCTGATGTCATGTGTATGGCATGTGGTTTTACATCAACAACTTATGATCCATGTGTAGACATCTCTTTGGATTTGGAACCAAATCTAGCGGGATCTGCGAAGATGGCGGCCACAAGGTCCAATCTTCCTTGCAATGGCGAGGCAGATTGCATGAACTCGAGCCAAAACCAGCGACTATCTTCCCTGGTGGGTTGTTTGGATCAATTCACTAGACCCGAAAAACTCGGGTCCGACCAAAAATTCTTCTGCCAACAGTGTCAAGTGCGGCAGGAATCGCTCAAACAGATGTCCATAAGAAAGCTTCCATTAGTTTCTTGTTTCCACATTAAAAGATTTGAGCATTCTTCAGTTCGAAAAATGTCGAGGAAGGTGGACCGATATTTGCAGTTTCCGTTTTCTTTAGACATGGCACCCTATCTCTCATCTTCAATCTTGAGAAATCGATTTGGTAATAGGATTTTTCCTTTCGATGGAGATGAACAAGATGGTTGTAACGAGACATCGTCTGAGTTCGAGTTGTTTGCCGTTATCACTCACAAAGGAAAATTAGACGCTGGTCATTACGTGACCTACTTGCGGCTAAGTAATCAATGGTATAAGTGCGACGATGCTTGGATTACCCAAGTCAACGAAAACATCGTCAGGGCTGCACAAGGATACATGATGTTTTATGTGCAGAAGATGCTCTATTACAAAGCAAGTGAGAAACAGAATGCTTCATAA

mRNA sequence

CTTCTTCTCACCTTCTTACCCTCCTTCCCCATCGCTTTCACCTCCCCTCCCATGTCTTCCAATTTCCACCACCACCATCGTCACCACCCAAATCTTCCTTTCAACTCTCAGATCTTCCCCCCTTCTTGTCCCCACCTCGCCGACTTCCGTTCTCGAAATGGTTCCAAGTCCTTCCGCCTTCTACAAGACTGTCTTCGCGTCAAGCCCCCCGGTGGCCGCGCCGCCATCCGTCGAGACCCCAACGAGGTTCCCCGATGTGGTTCCTGCGGTGATTCCGCTCGTCCAAGGCTATATGCTTGTGTGACCTGCGCCGCCGTGTCCTGTCACGCGCCGCCCTCCCATTCCCATGCCGCGGCTCACGCCGAATCAATGCCGCCAGGTCATGAGATCGCTGTCGACGTCGATCGTGCCGAGCTTTTTTGCTGTGCTTGCCGCGACCAGGTTTATGATCGCGACTTTGACGCTGCAGTGGTGTTGGCTCAAACGACGGCGTCTACTCTTGGCGGGAATGCGGTCTCTTCCAGTGGAGCACCGCCGTCGCAGCCTCTGCTCCCGCCGGAGAATCTGAGGAAGCGGCGGAGGGTTGATTATCAGCCGTGGGTACCGGATCTGAGGGAGAGGGTTTTAGTGGGTGCTAATTCGAGTCCACTTGATTGTGCGAATTCCAATCCGGGTTCTGAATCTGAGTTGCCCTGGGGGTTGCGGGGATTGAACAATTTGGGGAATACGTGTTTTATGAACTCGGTCTTGCAAGCATTGCTGCATACTCCGCCGTTGAGGAATTTTTTCTTGAGTGATCGTCACAATCGCTATTTTTGTCAGAGAAATGTCAATGTGGGCAATAAAAATTCGAATGCCAACGATGGAAATGGCAGTAAAAATTCGCGCAGCTGTCTGGCTTGTGATCTGGATTCTATATTTTCTGCTGTCTTCTCCGGTGATCGGATCCCTTACAGCCCAGCAAAATTTTTATTGAGCTGGTGGCAACATGCTGCAAACCTCGCAAGTTATGAACAACAAGATGCACACGAATTTTTCATCTCTATTCTCGAAGGCATCCACGAAAAGGTAGACAAAGATCGGCGAAAACCTTACAGTCAAGGCAATGGAGACTGTTGTATTGCTCATAGAGTATTTTCTGGCATACTGCGATCTGATGTCATGTGTATGGCATGTGGTTTTACATCAACAACTTATGATCCATGTGTAGACATCTCTTTGGATTTGGAACCAAATCTAGCGGGATCTGCGAAGATGGCGGCCACAAGGTCCAATCTTCCTTGCAATGGCGAGGCAGATTGCATGAACTCGAGCCAAAACCAGCGACTATCTTCCCTGGTGGGTTGTTTGGATCAATTCACTAGACCCGAAAAACTCGGGTCCGACCAAAAATTCTTCTGCCAACAGTGTCAAGTGCGGCAGGAATCGCTCAAACAGATGTCCATAAGAAAGCTTCCATTAGTTTCTTGTTTCCACATTAAAAGATTTGAGCATTCTTCAGTTCGAAAAATGTCGAGGAAGGTGGACCGATATTTGCAGTTTCCGTTTTCTTTAGACATGGCACCCTATCTCTCATCTTCAATCTTGAGAAATCGATTTGGTAATAGGATTTTTCCTTTCGATGGAGATGAACAAGATGGTTGTAACGAGACATCGTCTGAGTTCGAGTTGTTTGCCGTTATCACTCACAAAGGAAAATTAGACGCTGGTCATTACGTGACCTACTTGCGGCTAAGTAATCAATGGTATAAGTGCGACGATGCTTGGATTACCCAAGTCAACGAAAACATCGTCAGGGCTGCACAAGGATACATGATGTTTTATGTGCAGAAGATGCTCTATTACAAAGCAAGTGAGAAACAGAATGCTTCATAA

Coding sequence (CDS)

ATGTCTTCCAATTTCCACCACCACCATCGTCACCACCCAAATCTTCCTTTCAACTCTCAGATCTTCCCCCCTTCTTGTCCCCACCTCGCCGACTTCCGTTCTCGAAATGGTTCCAAGTCCTTCCGCCTTCTACAAGACTGTCTTCGCGTCAAGCCCCCCGGTGGCCGCGCCGCCATCCGTCGAGACCCCAACGAGGTTCCCCGATGTGGTTCCTGCGGTGATTCCGCTCGTCCAAGGCTATATGCTTGTGTGACCTGCGCCGCCGTGTCCTGTCACGCGCCGCCCTCCCATTCCCATGCCGCGGCTCACGCCGAATCAATGCCGCCAGGTCATGAGATCGCTGTCGACGTCGATCGTGCCGAGCTTTTTTGCTGTGCTTGCCGCGACCAGGTTTATGATCGCGACTTTGACGCTGCAGTGGTGTTGGCTCAAACGACGGCGTCTACTCTTGGCGGGAATGCGGTCTCTTCCAGTGGAGCACCGCCGTCGCAGCCTCTGCTCCCGCCGGAGAATCTGAGGAAGCGGCGGAGGGTTGATTATCAGCCGTGGGTACCGGATCTGAGGGAGAGGGTTTTAGTGGGTGCTAATTCGAGTCCACTTGATTGTGCGAATTCCAATCCGGGTTCTGAATCTGAGTTGCCCTGGGGGTTGCGGGGATTGAACAATTTGGGGAATACGTGTTTTATGAACTCGGTCTTGCAAGCATTGCTGCATACTCCGCCGTTGAGGAATTTTTTCTTGAGTGATCGTCACAATCGCTATTTTTGTCAGAGAAATGTCAATGTGGGCAATAAAAATTCGAATGCCAACGATGGAAATGGCAGTAAAAATTCGCGCAGCTGTCTGGCTTGTGATCTGGATTCTATATTTTCTGCTGTCTTCTCCGGTGATCGGATCCCTTACAGCCCAGCAAAATTTTTATTGAGCTGGTGGCAACATGCTGCAAACCTCGCAAGTTATGAACAACAAGATGCACACGAATTTTTCATCTCTATTCTCGAAGGCATCCACGAAAAGGTAGACAAAGATCGGCGAAAACCTTACAGTCAAGGCAATGGAGACTGTTGTATTGCTCATAGAGTATTTTCTGGCATACTGCGATCTGATGTCATGTGTATGGCATGTGGTTTTACATCAACAACTTATGATCCATGTGTAGACATCTCTTTGGATTTGGAACCAAATCTAGCGGGATCTGCGAAGATGGCGGCCACAAGGTCCAATCTTCCTTGCAATGGCGAGGCAGATTGCATGAACTCGAGCCAAAACCAGCGACTATCTTCCCTGGTGGGTTGTTTGGATCAATTCACTAGACCCGAAAAACTCGGGTCCGACCAAAAATTCTTCTGCCAACAGTGTCAAGTGCGGCAGGAATCGCTCAAACAGATGTCCATAAGAAAGCTTCCATTAGTTTCTTGTTTCCACATTAAAAGATTTGAGCATTCTTCAGTTCGAAAAATGTCGAGGAAGGTGGACCGATATTTGCAGTTTCCGTTTTCTTTAGACATGGCACCCTATCTCTCATCTTCAATCTTGAGAAATCGATTTGGTAATAGGATTTTTCCTTTCGATGGAGATGAACAAGATGGTTGTAACGAGACATCGTCTGAGTTCGAGTTGTTTGCCGTTATCACTCACAAAGGAAAATTAGACGCTGGTCATTACGTGACCTACTTGCGGCTAAGTAATCAATGGTATAAGTGCGACGATGCTTGGATTACCCAAGTCAACGAAAACATCGTCAGGGCTGCACAAGGATACATGATGTTTTATGTGCAGAAGATGCTCTATTACAAAGCAAGTGAGAAACAGAATGCTTCATAA

Protein sequence

MSSNFHHHHRHHPNLPFNSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDYQPWVPDLRERVLVGANSSPLDCANSNPGSESELPWGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIPYSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLAGSAKMAATRSNLPCNGEADCMNSSQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQNAS
Homology
BLAST of Cp4.1LG11g10480 vs. ExPASy Swiss-Prot
Match: Q9LEW0 (Ubiquitin C-terminal hydrolase 22 OS=Arabidopsis thaliana OX=3702 GN=UBP22 PE=1 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 1.1e-142
Identity = 286/590 (48.47%), Postives = 361/590 (61.19%), Query Frame = 0

Query: 23  PPSCPHLADFRSR---NGSKSFRLLQDCLRVKPPGGRAAIRRDPNEVPRCGSCGDSARPR 82
           P  C HL+D++ R   +G KSF  L  C        R  I+    ++PRC  C    + R
Sbjct: 11  PDPCNHLSDYKLRYGTDGYKSFNNLFRCFN----DARIKIKLQGIDIPRCSYC-SVYQKR 70

Query: 83  LYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQVYDRDFDAA 142
           LY C+ C ++SC      SH   H + +  GH+IA+DV+R+EL+CCAC DQVYD +FD  
Sbjct: 71  LYICLICRSISC-----SSHILLHTQ-LNKGHDIAIDVERSELYCCACIDQVYDSEFDEV 130

Query: 143 VVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDYQPWVPDLRERVLVGAN--S 202
           VV  Q      G      SGA      +      K+RR+D Q         +++G+N   
Sbjct: 131 VVSKQ----LFGLGMSVKSGAD-----VVAVRSNKKRRLDSQ---------LIIGSNFLV 190

Query: 203 SPLDCANSNPGSESELPWGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQ 262
           SP D        +   P GLRGLNNLG+TCFMN+VLQAL+H PPLRNF+LS +HNR  C 
Sbjct: 191 SPRD-----RREKWTFPLGLRGLNNLGSTCFMNAVLQALVHAPPLRNFWLSGQHNRDLCP 250

Query: 263 RNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIPYSPAKFLLSWWQHAANL 322
           R                      CL CDLD IFSA+FSGDR PYSPA  L SWWQH+ NL
Sbjct: 251 RRT----------------MGLLCLPCDLDVIFSAMFSGDRTPYSPAHLLYSWWQHSTNL 310

Query: 323 ASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDC-CIAHRVFSGILRSDVMCMACG 382
           A+YEQQD+HEFFIS+L+ IHE  ++ + K   Q N +C CI H+ FSG+LRSDV C  CG
Sbjct: 311 ATYEQQDSHEFFISLLDRIHE--NEGKSKCLYQDNEECQCITHKAFSGLLRSDVTCTTCG 370

Query: 383 FTSTTYDPCVDISLDLEPNLAGSAKMAATRSNLPCNGEADCMNSSQNQRLSSLVGCLDQF 442
            TSTTYDP +DISL L+         A  R N    G       S N  + +L GCLD F
Sbjct: 371 STSTTYDPFIDISLTLDS--MNGFSPADCRKNRYSGG------PSVNAIMPTLSGCLDFF 430

Query: 443 TRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQ 502
           TR EKLG DQK  CQ C  ++ES KQMSIR+LPL+ C H+KRFEHS  RK SRK+D YLQ
Sbjct: 431 TRSEKLGPDQKLNCQSCGEKRESSKQMSIRRLPLLLCLHVKRFEHSLTRKTSRKIDSYLQ 490

Query: 503 FPFSLDMAPYLSSSILRNRFGNRIFPFDGD---EQDGCNETSSEFELFAVITHKGKLDAG 562
           +PF L+M+PYLSSSI+  RFGNRIF FDG+   +    +  S+EFE+FAV+THKG L++G
Sbjct: 491 YPFRLNMSPYLSSSIIGKRFGNRIFAFDGEGEYDSSSSSSPSAEFEIFAVVTHKGMLESG 540

Query: 563 HYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEK 604
           HYVTYLRL   WY+CDDAWI +V E +VR  + YM+FY Q+ +  KA ++
Sbjct: 551 HYVTYLRLKGLWYRCDDAWINEVEEEVVRGCECYMLFYAQETVIQKAHKE 540

BLAST of Cp4.1LG11g10480 vs. ExPASy Swiss-Prot
Match: Q5DU02 (Ubiquitin carboxyl-terminal hydrolase 22 OS=Mus musculus OX=10090 GN=Usp22 PE=2 SV=2)

HSP 1 Score: 328.9 bits (842), Expect = 1.2e-88
Identity = 205/582 (35.22%), Postives = 291/582 (50.00%), Query Frame = 0

Query: 23  PPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDPNEVPRCGSCGDSARPRLYA 82
           PP C HL  F+  N  ++ R +  C       G A  R+   +   C  CG     RL++
Sbjct: 20  PPGCSHLGSFKVDNWKQNLRAIYQCF---VWSGTAEARKRKAKSCVCHVCGIHLN-RLHS 79

Query: 83  CVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQVYDRDFDAAVVL 142
           C+ C    C    +  H   HA+S    H +A+D+    ++C  C+D +YD+D +     
Sbjct: 80  CLYCVFFGCF---TKKHIHDHAKS--KRHNLAIDLMYGGIYCFLCQDYIYDKDIEIIAKE 139

Query: 143 AQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDYQPWVPDLRERVLVGANSSPLDC 202
            Q  A  + G                           +  W P  RE          L+ 
Sbjct: 140 EQRKAWKMQGVGEK-----------------------FSTWEPTKRE----------LEL 199

Query: 203 ANSNPGSE---SELPWGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQRN 262
              NP      S    GLRGL NLGNTCFMN ++QAL HTP LR+FFLSDRH    C+  
Sbjct: 200 LKHNPKRRKITSNCTIGLRGLINLGNTCFMNCIVQALTHTPLLRDFFLSDRHR---CE-- 259

Query: 263 VNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIPYSPAKFLLSWWQHAANLAS 322
                           ++  SCL C++ S+F   +SG R P+ P K L   W HA +LA 
Sbjct: 260 ---------------MQSPSSCLVCEMSSLFQEFYSGHRSPHIPYKLLHLVWTHARHLAG 319

Query: 323 YEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDC-CIAHRVFSGILRSDVMCMACGFT 382
           YEQQDAHEF I+ L+ +H     D     +     C CI  ++F+G L+SDV C  C   
Sbjct: 320 YEQQDAHEFLIAALDVLHRHCKGDDNGKKANNPNHCNCIIDQIFTGGLQSDVTCQVCHGV 379

Query: 383 STTYDPCVDISLDLEPNLAGSAKMAATRSNLPCNGEADCMNSSQNQRLSSLVGCLDQFTR 442
           STT DP  DISLDL  +      ++        NGE+    +      ++L  CL +FTR
Sbjct: 380 STTIDPFWDISLDLPGSSTPFWPLSPGSEGSVVNGESHASGT------TTLTDCLRRFTR 439

Query: 443 PEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFP 502
           PE LGS  K  C  C   QES KQ++++KLP+V+CFH+KRFEHS+  K+ RK+  Y+ FP
Sbjct: 440 PEHLGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHLKRFEHSA--KLRRKITTYVSFP 499

Query: 503 FSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFELFAVITHKGKLDAGHYVTY 562
             LDM P+++SS      G    P D    D      +++ LFAV+ H+G L++GHY ++
Sbjct: 500 LELDMTPFMASSKESRMNGQYQQPLDSLNND------NKYSLFAVVNHQGTLESGHYTSF 525

Query: 563 LRL-SNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYK 600
           +R   +QW+KCDDA IT+ +   V  ++GY++FY ++ L Y+
Sbjct: 560 IRQHKDQWFKCDDAIITKASIKDVLDSEGYLLFYHKQFLEYE 525

BLAST of Cp4.1LG11g10480 vs. ExPASy Swiss-Prot
Match: Q9UPT9 (Ubiquitin carboxyl-terminal hydrolase 22 OS=Homo sapiens OX=9606 GN=USP22 PE=1 SV=2)

HSP 1 Score: 327.8 bits (839), Expect = 2.6e-88
Identity = 205/584 (35.10%), Postives = 291/584 (49.83%), Query Frame = 0

Query: 21  IFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDPNEVPRCGSCGDSARPRL 80
           + PP C HL  F+  N  ++ R +  C       G A  R+   +   C  CG     RL
Sbjct: 18  VAPPGCSHLGSFKVDNWKQNLRAIYQCF---VWSGTAEARKRKAKSCICHVCGVHLN-RL 77

Query: 81  YACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQVYDRDFDAAV 140
           ++C+ C    C     H H  A A+     H +A+D+    ++C  C+D +YD+D +   
Sbjct: 78  HSCLYCVFFGCFT-KKHIHEHAKAKR----HNLAIDLMYGGIYCFLCQDYIYDKDMEIIA 137

Query: 141 VLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDYQPWVPDLRERVLVGANSSPL 200
              Q  A  + G                           +  W P  RE          L
Sbjct: 138 KEEQRKAWKMQGVGEK-----------------------FSTWEPTKRE----------L 197

Query: 201 DCANSNPGSE---SELPWGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQ 260
           +    NP      S    GLRGL NLGNTCFMN ++QAL HTP LR+FFLSDRH    C+
Sbjct: 198 ELLKHNPKRRKITSNCTIGLRGLINLGNTCFMNCIVQALTHTPLLRDFFLSDRHR---CE 257

Query: 261 RNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIPYSPAKFLLSWWQHAANL 320
                             ++  SCL C++ S+F   +SG R P+ P K L   W HA +L
Sbjct: 258 -----------------MQSPSSCLVCEMSSLFQEFYSGHRSPHIPYKLLHLVWTHARHL 317

Query: 321 ASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDC-CIAHRVFSGILRSDVMCMACG 380
           A YEQQDAHEF I+ L+ +H     D     +     C CI  ++F+G L+SDV C  C 
Sbjct: 318 AGYEQQDAHEFLIAALDVLHRHCKGDDNGKKANNPNHCNCIIDQIFTGGLQSDVTCQVCH 377

Query: 381 FTSTTYDPCVDISLDLEPNLAGSAKMAATRSNLPCNGEADCMNSSQNQRLSSLVGCLDQF 440
             STT DP  DISLDL  +      ++        NGE+    +      ++L  CL +F
Sbjct: 378 GVSTTIDPFWDISLDLPGSSTPFWPLSPGSEGNVVNGESHVSGT------TTLTDCLRRF 437

Query: 441 TRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQ 500
           TRPE LGS  K  C  C   QES KQ++++KLP+V+CFH+KRFEHS+  K+ RK+  Y+ 
Sbjct: 438 TRPEHLGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHLKRFEHSA--KLRRKITTYVS 497

Query: 501 FPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFELFAVITHKGKLDAGHYV 560
           FP  LDM P+++SS      G    P D    D      +++ LFAV+ H+G L++GHY 
Sbjct: 498 FPLELDMTPFMASSKESRMNGQYQQPTDSLNND------NKYSLFAVVNHQGTLESGHYT 525

Query: 561 TYLRL-SNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYK 600
           +++R   +QW+KCDDA IT+ +   V  ++GY++FY ++ L Y+
Sbjct: 558 SFIRQHKDQWFKCDDAIITKASIKDVLDSEGYLLFYHKQFLEYE 525

BLAST of Cp4.1LG11g10480 vs. ExPASy Swiss-Prot
Match: A6H8I0 (Ubiquitin carboxyl-terminal hydrolase 22 OS=Danio rerio OX=7955 GN=usp22 PE=2 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 3.4e-88
Identity = 204/581 (35.11%), Postives = 289/581 (49.74%), Query Frame = 0

Query: 23  PPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDPNEVPRCGSCGDSARPRLYA 82
           P  C H+  F+  N  ++ R++  C       G A  R+   +   C  CG     RL++
Sbjct: 3   PAGCSHVNGFKVDNWKQNLRVIYQCF---VWSGSAETRKRKAKSCICHMCGAHLN-RLHS 62

Query: 83  CVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQVYDRDFDAAVVL 142
           C+ C    C    S  H   HA++    H +A+D+    ++C  C+D +YD+D +     
Sbjct: 63  CLHCVFFGCF---SKKHIHEHAKN--KRHNLAIDLLYGGIYCFVCQDYIYDKDMEQIAKE 122

Query: 143 AQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDYQPWVPDLRERVLVGANSSPLDC 202
            Q  A  L G                           Y  W P  RE          L+ 
Sbjct: 123 EQRKAWKLQGIGEK-----------------------YSMWEPTKRE----------LEL 182

Query: 203 ANSNPGSE---SELPWGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQRN 262
              NP      +    GLRGL NLGNTCFMN ++QAL HTP LR+FFLSDRH    C+  
Sbjct: 183 LRHNPKRRKITANCTIGLRGLINLGNTCFMNCIVQALTHTPLLRDFFLSDRHK---CE-- 242

Query: 263 VNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIPYSPAKFLLSWWQHAANLAS 322
                             S SCL C++  +F   +SG R P+ P + L   W HA +LA 
Sbjct: 243 ----------------MQSNSCLVCEMSQLFQEFYSGHRSPHIPFRLLHLVWTHARHLAG 302

Query: 323 YEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHRVFSGILRSDVMCMACGFTS 382
           YEQQDAHEF I+ L+ +H     D  K  +  N   CI  ++F+G L+SDV C  C   S
Sbjct: 303 YEQQDAHEFLIAALDVLHRHCKDDNGKKANNPNHCNCIIDQIFTGGLQSDVTCQVCHGVS 362

Query: 383 TTYDPCVDISLDLEPNLAGSAKMAATRSNLPCNGEADCMNSSQNQRLSSLVGCLDQFTRP 442
           TT DP  DISLDL  +      ++        NG+      S     ++L  CL +FTRP
Sbjct: 363 TTIDPFWDISLDLPGSSTPFWPLSPGSDGSVVNGD------SHPSGATTLTDCLRRFTRP 422

Query: 443 EKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPF 502
           E LGS  K  C  C   QES KQ+++++LP+V+CFH+KRFEHS+  K+ RK+  Y+ FP 
Sbjct: 423 EHLGSSAKIKCSGCHSYQESTKQLTMKRLPIVACFHLKRFEHSA--KLRRKITTYVSFPL 482

Query: 503 SLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFELFAVITHKGKLDAGHYVTYL 562
            LDM P+++SS      G    P D    D      +++ LFAV+ H+G L++GHY T++
Sbjct: 483 ELDMTPFMASSKESRMNGQYQQPVDSLNND------NKYSLFAVVNHQGTLESGHYTTFI 506

Query: 563 RL-SNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYK 600
           R   +QW+KCDDA IT+ +   V  ++GY++FY ++ L Y+
Sbjct: 543 RQHKDQWFKCDDAIITKASIKDVLDSEGYLLFYHKQFLEYE 506

BLAST of Cp4.1LG11g10480 vs. ExPASy Swiss-Prot
Match: P0C8Z3 (Ubiquitin carboxyl-terminal hydrolase 22 OS=Bos taurus OX=9913 GN=USP22 PE=2 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 5.8e-88
Identity = 204/584 (34.93%), Postives = 293/584 (50.17%), Query Frame = 0

Query: 21  IFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDPNEVPRCGSCGDSARPRL 80
           + PP C HL  F+  N  ++ R +  C       G A  R+   +   C  CG     RL
Sbjct: 7   VTPPGCAHLGSFKVDNWKQNLRAIYQCF---VWSGSAEARKRKAKSCVCHVCGLHLN-RL 66

Query: 81  YACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQVYDRDFDAAV 140
           ++C+ C    C    +  H   HA+S    H +A+++    ++C  C+D +YD+D +   
Sbjct: 67  HSCLHCVFFGCF---TKKHIHEHAKS--KRHNLAIELMYGGIYCFLCQDYIYDKDIEIIA 126

Query: 141 VLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDYQPWVPDLRERVLVGANSSPL 200
              Q  A  + G                           +  W P  RE          L
Sbjct: 127 KEEQRKAWKMQGVGEK-----------------------FSTWEPTKRE----------L 186

Query: 201 DCANSNPGSE---SELPWGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQ 260
           +    NP      S    GLRGL NLGNTCFMN ++QAL HTP LR+FFLSDRH    C+
Sbjct: 187 ELLKHNPKRRKITSNCTIGLRGLINLGNTCFMNCIVQALTHTPLLRDFFLSDRHR---CE 246

Query: 261 RNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIPYSPAKFLLSWWQHAANL 320
                             ++  SCL C++ S+F   +SG R P+ P K L   W HA +L
Sbjct: 247 -----------------MQSPSSCLVCEMSSLFQEFYSGHRSPHIPYKLLHLVWTHARHL 306

Query: 321 ASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDC-CIAHRVFSGILRSDVMCMACG 380
           A YEQQDAHEF I+ L+ +H     D     +     C CI  ++F+G L+SDV C  C 
Sbjct: 307 AGYEQQDAHEFLIAALDVLHRHCKGDDNGKKANNPNHCNCIIDQIFTGGLQSDVTCQVCH 366

Query: 381 FTSTTYDPCVDISLDLEPNLAGSAKMAATRSNLPCNGEADCMNSSQNQRLSSLVGCLDQF 440
             STT DP  DISLDL  +      ++    +   NGE+    +      ++L  CL +F
Sbjct: 367 GVSTTIDPFWDISLDLPGSSTPFWPLSPGSESSVVNGESHVSGT------TTLTDCLRRF 426

Query: 441 TRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQ 500
           TRPE LGS  K  C  C   QES KQ++++KLP+V+CFH+KRFEHS+  K+ RK+  Y+ 
Sbjct: 427 TRPEHLGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHLKRFEHSA--KLRRKITTYVS 486

Query: 501 FPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFELFAVITHKGKLDAGHYV 560
           FP  LDM P+++SS      G    P D    D      +++ LFAV+ H+G L++GHY 
Sbjct: 487 FPLELDMTPFMASSKESRMNGQYQQPTDSLNND------NKYSLFAVVNHQGTLESGHYT 514

Query: 561 TYLRL-SNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYK 600
           +++R   +QW+KCDDA IT+ +   V  ++GY++FY ++ L Y+
Sbjct: 547 SFIRQHKDQWFKCDDAIITKASIADVLDSEGYLLFYHKQFLEYE 514

BLAST of Cp4.1LG11g10480 vs. NCBI nr
Match: XP_022946305.1 (ubiquitin carboxyl-terminal hydrolase 22-like [Cucurbita moschata] >XP_023547055.1 ubiquitin carboxyl-terminal hydrolase 22-like [Cucurbita pepo subsp. pepo] >KAG7030363.1 Ubiquitin carboxyl-terminal hydrolase 22 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1256 bits (3251), Expect = 0.0
Identity = 607/607 (100.00%), Postives = 607/607 (100.00%), Query Frame = 0

Query: 1   MSSNFHHHHRHHPNLPFNSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR 60
           MSSNFHHHHRHHPNLPFNSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR
Sbjct: 1   MSSNFHHHHRHHPNLPFNSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR 60

Query: 61  RDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRA 120
           RDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRA
Sbjct: 61  RDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRA 120

Query: 121 ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDY 180
           ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDY
Sbjct: 121 ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDY 180

Query: 181 QPWVPDLRERVLVGANSSPLDCANSNPGSESELPWGLRGLNNLGNTCFMNSVLQALLHTP 240
           QPWVPDLRERVLVGANSSPLDCANSNPGSESELPWGLRGLNNLGNTCFMNSVLQALLHTP
Sbjct: 181 QPWVPDLRERVLVGANSSPLDCANSNPGSESELPWGLRGLNNLGNTCFMNSVLQALLHTP 240

Query: 241 PLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIP 300
           PLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIP
Sbjct: 241 PLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIP 300

Query: 301 YSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR 360
           YSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR
Sbjct: 301 YSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR 360

Query: 361 VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLAGSAKMAATRSNLPCNGEADCMNS 420
           VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLAGSAKMAATRSNLPCNGEADCMNS
Sbjct: 361 VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLAGSAKMAATRSNLPCNGEADCMNS 420

Query: 421 SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE 480
           SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE
Sbjct: 421 SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE 480

Query: 481 HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFEL 540
           HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFEL
Sbjct: 481 HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFEL 540

Query: 541 FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA 600
           FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA
Sbjct: 541 FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA 600

Query: 601 SEKQNAS 607
           SEKQNAS
Sbjct: 601 SEKQNAS 607

BLAST of Cp4.1LG11g10480 vs. NCBI nr
Match: XP_022999050.1 (ubiquitin carboxyl-terminal hydrolase 22-like [Cucurbita maxima])

HSP 1 Score: 1238 bits (3204), Expect = 0.0
Identity = 600/607 (98.85%), Postives = 602/607 (99.18%), Query Frame = 0

Query: 1   MSSNFHHHHRHHPNLPFNSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR 60
           MSSNFHHHHRHHPNLPFNSQIFP SCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR
Sbjct: 1   MSSNFHHHHRHHPNLPFNSQIFPTSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR 60

Query: 61  RDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRA 120
           RDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRA
Sbjct: 61  RDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRA 120

Query: 121 ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDY 180
           ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDY
Sbjct: 121 ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDY 180

Query: 181 QPWVPDLRERVLVGANSSPLDCANSNPGSESELPWGLRGLNNLGNTCFMNSVLQALLHTP 240
           QPWVPDLRERVLVG NSSPLDCANSNPGSES LPWGLRGLNNLGNTCFMNSVLQALLHTP
Sbjct: 181 QPWVPDLRERVLVGTNSSPLDCANSNPGSESALPWGLRGLNNLGNTCFMNSVLQALLHTP 240

Query: 241 PLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIP 300
           PLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIP
Sbjct: 241 PLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIP 300

Query: 301 YSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR 360
           YSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR
Sbjct: 301 YSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR 360

Query: 361 VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLAGSAKMAATRSNLPCNGEADCMNS 420
           VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPN AGSAKMAATR NLPCNGEADCMNS
Sbjct: 361 VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNQAGSAKMAATR-NLPCNGEADCMNS 420

Query: 421 SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE 480
           SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE
Sbjct: 421 SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE 480

Query: 481 HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFEL 540
           HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILR+RFGNRIFPFDGDEQDGCNETSSEFEL
Sbjct: 481 HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDGCNETSSEFEL 540

Query: 541 FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA 600
           FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYK+
Sbjct: 541 FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKS 600

Query: 601 SEKQNAS 607
           SEKQNAS
Sbjct: 601 SEKQNAS 606

BLAST of Cp4.1LG11g10480 vs. NCBI nr
Match: XP_038889878.1 (ubiquitin C-terminal hydrolase 22-like [Benincasa hispida])

HSP 1 Score: 1219 bits (3154), Expect = 0.0
Identity = 589/607 (97.03%), Postives = 595/607 (98.02%), Query Frame = 0

Query: 1   MSSNFHHHHRHHPNLPFNSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR 60
           MSSNFHHHHRHHPNLPFNSQI PPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR
Sbjct: 1   MSSNFHHHHRHHPNLPFNSQISPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR 60

Query: 61  RDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRA 120
           RDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRA
Sbjct: 61  RDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRA 120

Query: 121 ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDY 180
           ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSG  PSQPLLPPENLRKRRRVDY
Sbjct: 121 ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGVSPSQPLLPPENLRKRRRVDY 180

Query: 181 QPWVPDLRERVLVGANSSPLDCANSNPGSESELPWGLRGLNNLGNTCFMNSVLQALLHTP 240
           QPW+PDLRERVLVGANSSPLDCANSN GSESELPWGLRGLNNLGNTCFMNSVLQALLHTP
Sbjct: 181 QPWIPDLRERVLVGANSSPLDCANSNLGSESELPWGLRGLNNLGNTCFMNSVLQALLHTP 240

Query: 241 PLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIP 300
           PLRNFFLSDRHNRYFCQRNVN GN NSNANDGNGSKNSR CLACDLD++FSAVFSGDR P
Sbjct: 241 PLRNFFLSDRHNRYFCQRNVNGGNINSNANDGNGSKNSRICLACDLDAMFSAVFSGDRTP 300

Query: 301 YSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR 360
           YSPAKFL SWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR
Sbjct: 301 YSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR 360

Query: 361 VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLAGSAKMAATRSNLPCNGEADCMNS 420
           VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNL GS+KMAA RSNL CNGEADCMNS
Sbjct: 361 VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGGSSKMAAARSNLSCNGEADCMNS 420

Query: 421 SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE 480
           SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE
Sbjct: 421 SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE 480

Query: 481 HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFEL 540
           HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILR+RFGNRIFPFDGDEQDGCNETSSEFEL
Sbjct: 481 HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDGCNETSSEFEL 540

Query: 541 FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA 600
           FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA
Sbjct: 541 FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA 600

Query: 601 SEKQNAS 607
           SEKQ+AS
Sbjct: 601 SEKQSAS 607

BLAST of Cp4.1LG11g10480 vs. NCBI nr
Match: XP_008457196.1 (PREDICTED: ubiquitin carboxyl-terminal hydrolase 22-like [Cucumis melo] >KAA0063520.1 ubiquitin carboxyl-terminal hydrolase 22-like [Cucumis melo var. makuwa] >TYJ97847.1 ubiquitin carboxyl-terminal hydrolase 22-like [Cucumis melo var. makuwa])

HSP 1 Score: 1198 bits (3100), Expect = 0.0
Identity = 582/607 (95.88%), Postives = 587/607 (96.71%), Query Frame = 0

Query: 1   MSSNFHHHHRHHPNLPFNSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR 60
           MSSNFHH     PNLPF SQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR
Sbjct: 1   MSSNFHH-----PNLPFTSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR 60

Query: 61  RDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRA 120
           RDPNEVPRC SCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMP GHEIAVDVDRA
Sbjct: 61  RDPNEVPRCASCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPTGHEIAVDVDRA 120

Query: 121 ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDY 180
           ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSG  PSQPLLPPENLRKRRRVDY
Sbjct: 121 ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGVSPSQPLLPPENLRKRRRVDY 180

Query: 181 QPWVPDLRERVLVGANSSPLDCANSNPGSESELPWGLRGLNNLGNTCFMNSVLQALLHTP 240
           QPW+PDLRERVLVGANSSPLDC NSN GSESELPWGLRGLNNLGNTCFMNSVLQALLHTP
Sbjct: 181 QPWMPDLRERVLVGANSSPLDCVNSNLGSESELPWGLRGLNNLGNTCFMNSVLQALLHTP 240

Query: 241 PLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIP 300
           PLRNFFLSDRHNRY+CQRNVN GN NSN NDGNGSKNSR CLACDLD++FSAVFSGDR P
Sbjct: 241 PLRNFFLSDRHNRYYCQRNVNGGNINSNVNDGNGSKNSRICLACDLDAMFSAVFSGDRTP 300

Query: 301 YSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR 360
           YSPAKFL SWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR
Sbjct: 301 YSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR 360

Query: 361 VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLAGSAKMAATRSNLPCNGEADCMNS 420
           VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNL GSAKMAA RSNLPCNGEADCMNS
Sbjct: 361 VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAAARSNLPCNGEADCMNS 420

Query: 421 SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE 480
           SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE
Sbjct: 421 SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE 480

Query: 481 HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFEL 540
           HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFEL
Sbjct: 481 HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFEL 540

Query: 541 FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA 600
           FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA
Sbjct: 541 FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA 600

Query: 601 SEKQNAS 607
           SEKQ+AS
Sbjct: 601 SEKQSAS 602

BLAST of Cp4.1LG11g10480 vs. NCBI nr
Match: XP_004139406.1 (ubiquitin C-terminal hydrolase 22 isoform X2 [Cucumis sativus] >KGN60716.1 hypothetical protein Csa_019516 [Cucumis sativus])

HSP 1 Score: 1186 bits (3069), Expect = 0.0
Identity = 577/607 (95.06%), Postives = 583/607 (96.05%), Query Frame = 0

Query: 1   MSSNFHHHHRHHPNLPFNSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR 60
           MSSNFHH     PNLPF SQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR
Sbjct: 1   MSSNFHH-----PNLPFTSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR 60

Query: 61  RDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRA 120
           RDPNEVPRC SCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMP GHEIAVDVDRA
Sbjct: 61  RDPNEVPRCSSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPTGHEIAVDVDRA 120

Query: 121 ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDY 180
           ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSG  PSQP LPPENLRKRRRVDY
Sbjct: 121 ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGVSPSQPSLPPENLRKRRRVDY 180

Query: 181 QPWVPDLRERVLVGANSSPLDCANSNPGSESELPWGLRGLNNLGNTCFMNSVLQALLHTP 240
           QPW+PDLRERVLVGANSSPLDC NSN GSESELPWGLRGLNNLGNTCFMNSVLQALLHTP
Sbjct: 181 QPWMPDLRERVLVGANSSPLDCVNSNLGSESELPWGLRGLNNLGNTCFMNSVLQALLHTP 240

Query: 241 PLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIP 300
           PLRNFFLSDRHNRYFCQRNVN  N +SN NDGNGSKNSR CLACDLD++FSAVFSGDR P
Sbjct: 241 PLRNFFLSDRHNRYFCQRNVNGSNVSSNVNDGNGSKNSRICLACDLDAMFSAVFSGDRTP 300

Query: 301 YSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR 360
           YSPAKFL SWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR
Sbjct: 301 YSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR 360

Query: 361 VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLAGSAKMAATRSNLPCNGEADCMNS 420
           VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNL GSAKMAA RSNLPCNGEADCMNS
Sbjct: 361 VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAAARSNLPCNGEADCMNS 420

Query: 421 SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE 480
           SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE
Sbjct: 421 SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE 480

Query: 481 HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFEL 540
           HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILR+RFGNRIFPFDGDE DGCNE SSEFEL
Sbjct: 481 HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDELDGCNEMSSEFEL 540

Query: 541 FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA 600
           FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA
Sbjct: 541 FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA 600

Query: 601 SEKQNAS 607
           SEKQ+AS
Sbjct: 601 SEKQSAS 602

BLAST of Cp4.1LG11g10480 vs. ExPASy TrEMBL
Match: A0A6J1G3H3 (Ubiquitin carboxyl-terminal hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450426 PE=3 SV=1)

HSP 1 Score: 1256 bits (3251), Expect = 0.0
Identity = 607/607 (100.00%), Postives = 607/607 (100.00%), Query Frame = 0

Query: 1   MSSNFHHHHRHHPNLPFNSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR 60
           MSSNFHHHHRHHPNLPFNSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR
Sbjct: 1   MSSNFHHHHRHHPNLPFNSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR 60

Query: 61  RDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRA 120
           RDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRA
Sbjct: 61  RDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRA 120

Query: 121 ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDY 180
           ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDY
Sbjct: 121 ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDY 180

Query: 181 QPWVPDLRERVLVGANSSPLDCANSNPGSESELPWGLRGLNNLGNTCFMNSVLQALLHTP 240
           QPWVPDLRERVLVGANSSPLDCANSNPGSESELPWGLRGLNNLGNTCFMNSVLQALLHTP
Sbjct: 181 QPWVPDLRERVLVGANSSPLDCANSNPGSESELPWGLRGLNNLGNTCFMNSVLQALLHTP 240

Query: 241 PLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIP 300
           PLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIP
Sbjct: 241 PLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIP 300

Query: 301 YSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR 360
           YSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR
Sbjct: 301 YSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR 360

Query: 361 VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLAGSAKMAATRSNLPCNGEADCMNS 420
           VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLAGSAKMAATRSNLPCNGEADCMNS
Sbjct: 361 VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLAGSAKMAATRSNLPCNGEADCMNS 420

Query: 421 SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE 480
           SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE
Sbjct: 421 SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE 480

Query: 481 HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFEL 540
           HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFEL
Sbjct: 481 HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFEL 540

Query: 541 FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA 600
           FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA
Sbjct: 541 FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA 600

Query: 601 SEKQNAS 607
           SEKQNAS
Sbjct: 601 SEKQNAS 607

BLAST of Cp4.1LG11g10480 vs. ExPASy TrEMBL
Match: A0A6J1KE79 (Ubiquitin carboxyl-terminal hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111493554 PE=3 SV=1)

HSP 1 Score: 1238 bits (3204), Expect = 0.0
Identity = 600/607 (98.85%), Postives = 602/607 (99.18%), Query Frame = 0

Query: 1   MSSNFHHHHRHHPNLPFNSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR 60
           MSSNFHHHHRHHPNLPFNSQIFP SCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR
Sbjct: 1   MSSNFHHHHRHHPNLPFNSQIFPTSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR 60

Query: 61  RDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRA 120
           RDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRA
Sbjct: 61  RDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRA 120

Query: 121 ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDY 180
           ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDY
Sbjct: 121 ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDY 180

Query: 181 QPWVPDLRERVLVGANSSPLDCANSNPGSESELPWGLRGLNNLGNTCFMNSVLQALLHTP 240
           QPWVPDLRERVLVG NSSPLDCANSNPGSES LPWGLRGLNNLGNTCFMNSVLQALLHTP
Sbjct: 181 QPWVPDLRERVLVGTNSSPLDCANSNPGSESALPWGLRGLNNLGNTCFMNSVLQALLHTP 240

Query: 241 PLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIP 300
           PLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIP
Sbjct: 241 PLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIP 300

Query: 301 YSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR 360
           YSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR
Sbjct: 301 YSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR 360

Query: 361 VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLAGSAKMAATRSNLPCNGEADCMNS 420
           VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPN AGSAKMAATR NLPCNGEADCMNS
Sbjct: 361 VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNQAGSAKMAATR-NLPCNGEADCMNS 420

Query: 421 SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE 480
           SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE
Sbjct: 421 SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE 480

Query: 481 HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFEL 540
           HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILR+RFGNRIFPFDGDEQDGCNETSSEFEL
Sbjct: 481 HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDGCNETSSEFEL 540

Query: 541 FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA 600
           FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYK+
Sbjct: 541 FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKS 600

Query: 601 SEKQNAS 607
           SEKQNAS
Sbjct: 601 SEKQNAS 606

BLAST of Cp4.1LG11g10480 vs. ExPASy TrEMBL
Match: A0A5D3BFE3 (Ubiquitin carboxyl-terminal hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G00590 PE=3 SV=1)

HSP 1 Score: 1198 bits (3100), Expect = 0.0
Identity = 582/607 (95.88%), Postives = 587/607 (96.71%), Query Frame = 0

Query: 1   MSSNFHHHHRHHPNLPFNSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR 60
           MSSNFHH     PNLPF SQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR
Sbjct: 1   MSSNFHH-----PNLPFTSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR 60

Query: 61  RDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRA 120
           RDPNEVPRC SCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMP GHEIAVDVDRA
Sbjct: 61  RDPNEVPRCASCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPTGHEIAVDVDRA 120

Query: 121 ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDY 180
           ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSG  PSQPLLPPENLRKRRRVDY
Sbjct: 121 ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGVSPSQPLLPPENLRKRRRVDY 180

Query: 181 QPWVPDLRERVLVGANSSPLDCANSNPGSESELPWGLRGLNNLGNTCFMNSVLQALLHTP 240
           QPW+PDLRERVLVGANSSPLDC NSN GSESELPWGLRGLNNLGNTCFMNSVLQALLHTP
Sbjct: 181 QPWMPDLRERVLVGANSSPLDCVNSNLGSESELPWGLRGLNNLGNTCFMNSVLQALLHTP 240

Query: 241 PLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIP 300
           PLRNFFLSDRHNRY+CQRNVN GN NSN NDGNGSKNSR CLACDLD++FSAVFSGDR P
Sbjct: 241 PLRNFFLSDRHNRYYCQRNVNGGNINSNVNDGNGSKNSRICLACDLDAMFSAVFSGDRTP 300

Query: 301 YSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR 360
           YSPAKFL SWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR
Sbjct: 301 YSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR 360

Query: 361 VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLAGSAKMAATRSNLPCNGEADCMNS 420
           VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNL GSAKMAA RSNLPCNGEADCMNS
Sbjct: 361 VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAAARSNLPCNGEADCMNS 420

Query: 421 SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE 480
           SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE
Sbjct: 421 SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE 480

Query: 481 HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFEL 540
           HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFEL
Sbjct: 481 HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFEL 540

Query: 541 FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA 600
           FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA
Sbjct: 541 FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA 600

Query: 601 SEKQNAS 607
           SEKQ+AS
Sbjct: 601 SEKQSAS 602

BLAST of Cp4.1LG11g10480 vs. ExPASy TrEMBL
Match: A0A1S3C4I9 (Ubiquitin carboxyl-terminal hydrolase OS=Cucumis melo OX=3656 GN=LOC103496929 PE=3 SV=1)

HSP 1 Score: 1198 bits (3100), Expect = 0.0
Identity = 582/607 (95.88%), Postives = 587/607 (96.71%), Query Frame = 0

Query: 1   MSSNFHHHHRHHPNLPFNSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR 60
           MSSNFHH     PNLPF SQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR
Sbjct: 1   MSSNFHH-----PNLPFTSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR 60

Query: 61  RDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRA 120
           RDPNEVPRC SCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMP GHEIAVDVDRA
Sbjct: 61  RDPNEVPRCASCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPTGHEIAVDVDRA 120

Query: 121 ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDY 180
           ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSG  PSQPLLPPENLRKRRRVDY
Sbjct: 121 ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGVSPSQPLLPPENLRKRRRVDY 180

Query: 181 QPWVPDLRERVLVGANSSPLDCANSNPGSESELPWGLRGLNNLGNTCFMNSVLQALLHTP 240
           QPW+PDLRERVLVGANSSPLDC NSN GSESELPWGLRGLNNLGNTCFMNSVLQALLHTP
Sbjct: 181 QPWMPDLRERVLVGANSSPLDCVNSNLGSESELPWGLRGLNNLGNTCFMNSVLQALLHTP 240

Query: 241 PLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIP 300
           PLRNFFLSDRHNRY+CQRNVN GN NSN NDGNGSKNSR CLACDLD++FSAVFSGDR P
Sbjct: 241 PLRNFFLSDRHNRYYCQRNVNGGNINSNVNDGNGSKNSRICLACDLDAMFSAVFSGDRTP 300

Query: 301 YSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR 360
           YSPAKFL SWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR
Sbjct: 301 YSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR 360

Query: 361 VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLAGSAKMAATRSNLPCNGEADCMNS 420
           VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNL GSAKMAA RSNLPCNGEADCMNS
Sbjct: 361 VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAAARSNLPCNGEADCMNS 420

Query: 421 SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE 480
           SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE
Sbjct: 421 SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE 480

Query: 481 HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFEL 540
           HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFEL
Sbjct: 481 HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFEL 540

Query: 541 FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA 600
           FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA
Sbjct: 541 FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA 600

Query: 601 SEKQNAS 607
           SEKQ+AS
Sbjct: 601 SEKQSAS 602

BLAST of Cp4.1LG11g10480 vs. ExPASy TrEMBL
Match: A0A0A0LL31 (Ubiquitin carboxyl-terminal hydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G008080 PE=3 SV=1)

HSP 1 Score: 1186 bits (3069), Expect = 0.0
Identity = 577/607 (95.06%), Postives = 583/607 (96.05%), Query Frame = 0

Query: 1   MSSNFHHHHRHHPNLPFNSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR 60
           MSSNFHH     PNLPF SQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR
Sbjct: 1   MSSNFHH-----PNLPFTSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIR 60

Query: 61  RDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRA 120
           RDPNEVPRC SCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMP GHEIAVDVDRA
Sbjct: 61  RDPNEVPRCSSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPTGHEIAVDVDRA 120

Query: 121 ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDY 180
           ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSG  PSQP LPPENLRKRRRVDY
Sbjct: 121 ELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGVSPSQPSLPPENLRKRRRVDY 180

Query: 181 QPWVPDLRERVLVGANSSPLDCANSNPGSESELPWGLRGLNNLGNTCFMNSVLQALLHTP 240
           QPW+PDLRERVLVGANSSPLDC NSN GSESELPWGLRGLNNLGNTCFMNSVLQALLHTP
Sbjct: 181 QPWMPDLRERVLVGANSSPLDCVNSNLGSESELPWGLRGLNNLGNTCFMNSVLQALLHTP 240

Query: 241 PLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIP 300
           PLRNFFLSDRHNRYFCQRNVN  N +SN NDGNGSKNSR CLACDLD++FSAVFSGDR P
Sbjct: 241 PLRNFFLSDRHNRYFCQRNVNGSNVSSNVNDGNGSKNSRICLACDLDAMFSAVFSGDRTP 300

Query: 301 YSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR 360
           YSPAKFL SWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR
Sbjct: 301 YSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHR 360

Query: 361 VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLAGSAKMAATRSNLPCNGEADCMNS 420
           VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNL GSAKMAA RSNLPCNGEADCMNS
Sbjct: 361 VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAAARSNLPCNGEADCMNS 420

Query: 421 SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE 480
           SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE
Sbjct: 421 SQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFE 480

Query: 481 HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFEL 540
           HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILR+RFGNRIFPFDGDE DGCNE SSEFEL
Sbjct: 481 HSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDELDGCNEMSSEFEL 540

Query: 541 FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA 600
           FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA
Sbjct: 541 FAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKA 600

Query: 601 SEKQNAS 607
           SEKQ+AS
Sbjct: 601 SEKQSAS 602

BLAST of Cp4.1LG11g10480 vs. TAIR 10
Match: AT5G10790.1 (ubiquitin-specific protease 22 )

HSP 1 Score: 508.4 bits (1308), Expect = 7.7e-144
Identity = 286/590 (48.47%), Postives = 361/590 (61.19%), Query Frame = 0

Query: 23  PPSCPHLADFRSR---NGSKSFRLLQDCLRVKPPGGRAAIRRDPNEVPRCGSCGDSARPR 82
           P  C HL+D++ R   +G KSF  L  C        R  I+    ++PRC  C    + R
Sbjct: 11  PDPCNHLSDYKLRYGTDGYKSFNNLFRCFN----DARIKIKLQGIDIPRCSYC-SVYQKR 70

Query: 83  LYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQVYDRDFDAA 142
           LY C+ C ++SC      SH   H + +  GH+IA+DV+R+EL+CCAC DQVYD +FD  
Sbjct: 71  LYICLICRSISC-----SSHILLHTQ-LNKGHDIAIDVERSELYCCACIDQVYDSEFDEV 130

Query: 143 VVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVDYQPWVPDLRERVLVGAN--S 202
           VV  Q      G      SGA      +      K+RR+D Q         +++G+N   
Sbjct: 131 VVSKQ----LFGLGMSVKSGAD-----VVAVRSNKKRRLDSQ---------LIIGSNFLV 190

Query: 203 SPLDCANSNPGSESELPWGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQ 262
           SP D        +   P GLRGLNNLG+TCFMN+VLQAL+H PPLRNF+LS +HNR  C 
Sbjct: 191 SPRD-----RREKWTFPLGLRGLNNLGSTCFMNAVLQALVHAPPLRNFWLSGQHNRDLCP 250

Query: 263 RNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRIPYSPAKFLLSWWQHAANL 322
           R                      CL CDLD IFSA+FSGDR PYSPA  L SWWQH+ NL
Sbjct: 251 RRT----------------MGLLCLPCDLDVIFSAMFSGDRTPYSPAHLLYSWWQHSTNL 310

Query: 323 ASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDC-CIAHRVFSGILRSDVMCMACG 382
           A+YEQQD+HEFFIS+L+ IHE  ++ + K   Q N +C CI H+ FSG+LRSDV C  CG
Sbjct: 311 ATYEQQDSHEFFISLLDRIHE--NEGKSKCLYQDNEECQCITHKAFSGLLRSDVTCTTCG 370

Query: 383 FTSTTYDPCVDISLDLEPNLAGSAKMAATRSNLPCNGEADCMNSSQNQRLSSLVGCLDQF 442
            TSTTYDP +DISL L+         A  R N    G       S N  + +L GCLD F
Sbjct: 371 STSTTYDPFIDISLTLDS--MNGFSPADCRKNRYSGG------PSVNAIMPTLSGCLDFF 430

Query: 443 TRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQ 502
           TR EKLG DQK  CQ C  ++ES KQMSIR+LPL+ C H+KRFEHS  RK SRK+D YLQ
Sbjct: 431 TRSEKLGPDQKLNCQSCGEKRESSKQMSIRRLPLLLCLHVKRFEHSLTRKTSRKIDSYLQ 490

Query: 503 FPFSLDMAPYLSSSILRNRFGNRIFPFDGD---EQDGCNETSSEFELFAVITHKGKLDAG 562
           +PF L+M+PYLSSSI+  RFGNRIF FDG+   +    +  S+EFE+FAV+THKG L++G
Sbjct: 491 YPFRLNMSPYLSSSIIGKRFGNRIFAFDGEGEYDSSSSSSPSAEFEIFAVVTHKGMLESG 540

Query: 563 HYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEK 604
           HYVTYLRL   WY+CDDAWI +V E +VR  + YM+FY Q+ +  KA ++
Sbjct: 551 HYVTYLRLKGLWYRCDDAWINEVEEEVVRGCECYMLFYAQETVIQKAHKE 540

BLAST of Cp4.1LG11g10480 vs. TAIR 10
Match: AT5G57990.1 (ubiquitin-specific protease 23 )

HSP 1 Score: 144.1 bits (362), Expect = 3.8e-34
Identity = 112/393 (28.50%), Postives = 172/393 (43.77%), Query Frame = 0

Query: 219 GLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRY-----FC-----QRNVNVGNKNSN 278
           GL NLGNTCF+NSVLQ L +T PL     +  H +Y     FC     Q++V        
Sbjct: 108 GLQNLGNTCFLNSVLQCLTYTEPLAATLQTAAHQKYCHVAGFCALCAIQKHVR------T 167

Query: 279 ANDGNGSKNSRSCLACDLDSIFSAVFSGDRIPYSPAKFLLSWWQHAANLASYEQQDAHEF 338
           A   NG   +   L  +L  I                        + N  +  Q+DAHE+
Sbjct: 168 ARQANGRILAPKDLVSNLRCI------------------------SRNFRNCRQEDAHEY 227

Query: 339 FISILEGIHE-----KVDKDRRKPYSQGNGDCCIAHRVFSGILRSDVMCMACGFTSTTYD 398
            I++LE +H+      V  +    Y +      + H++F G LRS V C  C   S  +D
Sbjct: 228 MINLLECMHKCSLPSGVPSESSDAYRRS-----LVHKIFGGSLRSQVKCEQCSHCSNKFD 287

Query: 399 PCVDISLDLEPNLAGSAKMAATRSNLPCNGEADCMNSSQNQRLSSLVGCLDQFTRPEKLG 458
           P +D+SLD+                                +  SL   L +FT  E L 
Sbjct: 288 PFLDLSLDI-------------------------------SKADSLQRALSRFTAVELLD 347

Query: 459 SDQKFF-CQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLD 518
           +  K + C++C+ + ++ KQ+++ K P V   H+KRFE     K+ RKVD    F  ++D
Sbjct: 348 NGAKVYQCERCKQKVKAKKQLTVSKAPYVLTVHLKRFEAHRSEKIDRKVD----FTSAID 407

Query: 519 MAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFELFAVITHKGKLD-AGHYVTYLRL 578
           M P++S                     G +E + ++ L+ V+ H G+   +GHY  ++R 
Sbjct: 408 MKPFVS---------------------GPHEGNLKYTLYGVLVHYGRSSHSGHYACFVRT 409

Query: 579 SN-QWYKCDDAWITQVNENIVRAAQGYMMFYVQ 594
           S+  WY  DD  + QV+E  V   + YM+FYV+
Sbjct: 468 SSGMWYSLDDNRVVQVSEKTVFNQKAYMLFYVR 409

BLAST of Cp4.1LG11g10480 vs. TAIR 10
Match: AT4G17895.1 (ubiquitin-specific protease 20 )

HSP 1 Score: 141.7 bits (356), Expect = 1.9e-33
Identity = 116/427 (27.17%), Postives = 195/427 (45.67%), Query Frame = 0

Query: 181 QPWVPDLRERVLVGANSSPLDCANSNPGSESELPWGLR-----------GLNNLGNTCFM 240
           +PW P++        +    D  NS       L +G R           GL NLGN+CF+
Sbjct: 129 RPWTPNVSPG-FGSDDDDDNDDDNSKNEPRKSLFYGFRQEPEPVTGVGAGLWNLGNSCFL 188

Query: 241 NSVLQALLHTPPLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSI 300
           NSV Q   HT PL    LS R+     +   + GN+               C+   +   
Sbjct: 189 NSVFQCFTHTVPLIESLLSFRY-----EVPCHCGNE-------------FFCVIRAIRYH 248

Query: 301 FSAVFSGDRIPYSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYS 360
             A    +R P +P  F  +    + +   Y+Q+DAHEF  + LE + E    DR    +
Sbjct: 249 IEAALRPERCPIAPYFFFDNLNYFSPDFQRYQQEDAHEFLQAFLEKL-EICGSDR----T 308

Query: 361 QGNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLAGSAKMAATRSNL 420
              GD   +  VFSG L S + C  C + S TY+  V +SL++E                
Sbjct: 309 SFRGD-ITSQDVFSGRLISGLRCCNCDYVSETYEKSVGLSLEIE---------------- 368

Query: 421 PCNGEADCMNSSQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLP 480
               + D + S+           L+ FTR EKL  D++  C  C  +    KQ+ + KLP
Sbjct: 369 ----DVDTLGSA-----------LESFTRVEKL--DEQLTCDNCNEKVSKEKQLLLDKLP 428

Query: 481 LVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQD 540
           LV+ FH+KRF+++ +     K+ ++++ P  +D+ PY+ +                  Q+
Sbjct: 429 LVATFHLKRFKNNGL--YMEKIYKHVKIPLEIDLQPYMRNI-----------------QE 476

Query: 541 GCNETSSEFELFAVITHKG-KLDAGHYVTYLRLSNQ-WYKCDDAWITQVNENIVRAAQGY 595
             NE S+++ L+A++ H G  +  GHY +Y+R + + W+  DD+ +T+++E++V +   Y
Sbjct: 489 --NEVSTKYHLYALVEHFGYSVAYGHYSSYVRSAPKIWHHFDDSKVTRIDEDMVLSQDSY 476

BLAST of Cp4.1LG11g10480 vs. TAIR 10
Match: AT3G14400.1 (ubiquitin-specific protease 25 )

HSP 1 Score: 129.4 bits (324), Expect = 9.7e-30
Identity = 98/378 (25.93%), Postives = 165/378 (43.65%), Query Frame = 0

Query: 219 GLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNS 278
           GL NLGNTC++NSVLQ L  TPPL NF L+ +H+ + C   V+              +  
Sbjct: 25  GLRNLGNTCYLNSVLQCLTFTPPLANFCLTHKHSSH-CDTYVD-------------GERK 84

Query: 279 RSCLACDLDSIFSAVFSGDRIPYSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHE 338
           R C  C ++   +   S D    +P K        A +     Q+DAHEF   +++  H 
Sbjct: 85  RDCPFCIVEKRIARSLSVDLTTDAPNKISSCLKIFAEHFKLGRQEDAHEFLRYVIDACHN 144

Query: 339 ---KVDKDRRKPYSQGNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPN 398
              ++ K R       NG+  +   +F G L+S V C++CG  S   D  +DISL++   
Sbjct: 145 TSLRLKKLRYNGNEPFNGN-SVVKEIFGGALQSQVKCLSCGAESNKADEIMDISLEI--- 204

Query: 399 LAGSAKMAATRSNLPCNGEADCMNSSQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQV 458
                                        + SS+   L +F + E L  + K+ C+ C+ 
Sbjct: 205 ----------------------------LQSSSVKESLQKFFQSEILDGNNKYRCESCEK 264

Query: 459 RQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNR 518
              + KQMSI + P +    +KRF          K+D+ + F   L ++ ++S +     
Sbjct: 265 LVTARKQMSILQAPNILVIQLKRFGGI----FGGKIDKAISFGEILVLSNFMSKA----- 324

Query: 519 FGNRIFPFDGDEQDGCNETSSEFELFAVITHKG-KLDAGHYVTYLRLS-NQWYKCDDAWI 578
                            +   E++LF +I H G   ++GHY  Y++ S  +WY C+D+++
Sbjct: 325 ---------------SKDPQPEYKLFGIIVHSGFSPESGHYYAYVKDSLGRWYCCNDSFV 332

Query: 579 TQVNENIVRAAQGYMMFY 592
           +      V + + Y++F+
Sbjct: 385 SLSTLQEVLSEKAYILFF 332

BLAST of Cp4.1LG11g10480 vs. TAIR 10
Match: AT2G24640.1 (ubiquitin-specific protease 19 )

HSP 1 Score: 129.0 bits (323), Expect = 1.3e-29
Identity = 104/386 (26.94%), Postives = 164/386 (42.49%), Query Frame = 0

Query: 219 GLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNS 278
           GL N GN+CF N VLQ L  T PL  + L   H R  C+RN                   
Sbjct: 175 GLTNCGNSCFANVVLQCLSWTRPLVAYLLERGHKRE-CRRN------------------- 234

Query: 279 RSCLACDLDSIFSAVFSGDRIPYSPAKFLLSWWQHAANLASYEQQDAHE---FFISILEG 338
             C  C+ ++      +  R P+SP   +        NL    Q+DAHE   F I +++ 
Sbjct: 235 DWCFLCEFENHLDRA-NYSRFPFSPMNIISRLPNIGGNLGYGRQEDAHELMRFAIDMMQS 294

Query: 339 I------HEKVDKDRRKPYSQGNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDIS 398
           +       EKV   R +       +  +   +F G+L+S V C AC   S  Y+  +D++
Sbjct: 295 VCLDEFGGEKVVPPRAQ-------ETTLIQYIFGGLLQSQVQCTACSNVSDQYENMMDLT 354

Query: 399 LDLEPNLAGSAKMAATRSNLPCNGEADCMNSSQNQRLSSLVGCLDQFTRPEKLGSDQKFF 458
           +++                   +G+A            SL  CLDQFT  E L  D  + 
Sbjct: 355 VEI-------------------HGDA-----------VSLEECLDQFTAKEWLQGDNLYK 414

Query: 459 CQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSS 518
           C +C    ++ K++SIR  P +    +KRF+     K+++++     FP + D+ PY+S 
Sbjct: 415 CDRCDDYVKACKRLSIRCAPNILTIALKRFQGGRFGKLNKRI----SFPETFDLGPYMS- 474

Query: 519 SILRNRFGNRIFPFDGDEQDGCNETSSEFELFAVITHKGKLDA---GHYVTYLR-LSNQW 578
                               G  E S  ++L+AVI H   L+A   GHY+ Y++     W
Sbjct: 475 --------------------GGGEGSDVYKLYAVIVHLDMLNASFFGHYICYVKDFRGNW 477

Query: 579 YKCDDAWITQVNENIVRAAQGYMMFY 592
           Y+ DD+ + +V    V + + YM+ Y
Sbjct: 535 YRIDDSEVEKVELEDVLSQRAYMLLY 477

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LEW01.1e-14248.47Ubiquitin C-terminal hydrolase 22 OS=Arabidopsis thaliana OX=3702 GN=UBP22 PE=1 ... [more]
Q5DU021.2e-8835.22Ubiquitin carboxyl-terminal hydrolase 22 OS=Mus musculus OX=10090 GN=Usp22 PE=2 ... [more]
Q9UPT92.6e-8835.10Ubiquitin carboxyl-terminal hydrolase 22 OS=Homo sapiens OX=9606 GN=USP22 PE=1 S... [more]
A6H8I03.4e-8835.11Ubiquitin carboxyl-terminal hydrolase 22 OS=Danio rerio OX=7955 GN=usp22 PE=2 SV... [more]
P0C8Z35.8e-8834.93Ubiquitin carboxyl-terminal hydrolase 22 OS=Bos taurus OX=9913 GN=USP22 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
XP_022946305.10.0100.00ubiquitin carboxyl-terminal hydrolase 22-like [Cucurbita moschata] >XP_023547055... [more]
XP_022999050.10.098.85ubiquitin carboxyl-terminal hydrolase 22-like [Cucurbita maxima][more]
XP_038889878.10.097.03ubiquitin C-terminal hydrolase 22-like [Benincasa hispida][more]
XP_008457196.10.095.88PREDICTED: ubiquitin carboxyl-terminal hydrolase 22-like [Cucumis melo] >KAA0063... [more]
XP_004139406.10.095.06ubiquitin C-terminal hydrolase 22 isoform X2 [Cucumis sativus] >KGN60716.1 hypot... [more]
Match NameE-valueIdentityDescription
A0A6J1G3H30.0100.00Ubiquitin carboxyl-terminal hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450... [more]
A0A6J1KE790.098.85Ubiquitin carboxyl-terminal hydrolase OS=Cucurbita maxima OX=3661 GN=LOC11149355... [more]
A0A5D3BFE30.095.88Ubiquitin carboxyl-terminal hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3C4I90.095.88Ubiquitin carboxyl-terminal hydrolase OS=Cucumis melo OX=3656 GN=LOC103496929 PE... [more]
A0A0A0LL310.095.06Ubiquitin carboxyl-terminal hydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G008080... [more]
Match NameE-valueIdentityDescription
AT5G10790.17.7e-14448.47ubiquitin-specific protease 22 [more]
AT5G57990.13.8e-3428.50ubiquitin-specific protease 23 [more]
AT4G17895.11.9e-3327.17ubiquitin-specific protease 20 [more]
AT3G14400.19.7e-3025.93ubiquitin-specific protease 25 [more]
AT2G24640.11.3e-2926.94ubiquitin-specific protease 19 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.90.70.10Cysteine proteinasescoord: 213..598
e-value: 3.8E-96
score: 324.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 151..174
NoneNo IPR availablePANTHERPTHR21646:SF66UBIQUITINYL HYDROLASE 1coord: 209..604
coord: 19..204
NoneNo IPR availablePANTHERPTHR21646UBIQUITIN CARBOXYL-TERMINAL HYDROLASEcoord: 209..604
NoneNo IPR availablePANTHERPTHR21646UBIQUITIN CARBOXYL-TERMINAL HYDROLASEcoord: 19..204
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 24..137
IPR001607Zinc finger, UBP-typePFAMPF02148zf-UBPcoord: 69..135
e-value: 4.9E-11
score: 42.8
IPR001607Zinc finger, UBP-typePROSITEPS50271ZF_UBPcoord: 67..133
score: 12.274639
IPR001394Peptidase C19, ubiquitin carboxyl-terminal hydrolasePFAMPF00443UCHcoord: 218..591
e-value: 2.4E-54
score: 184.6
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 22..150
e-value: 9.1E-21
score: 76.0
IPR018200Ubiquitin specific protease, conserved sitePROSITEPS00972USP_1coord: 219..234
IPR018200Ubiquitin specific protease, conserved sitePROSITEPS00973USP_2coord: 538..555
IPR028889Ubiquitin specific protease domainPROSITEPS50235USP_3coord: 218..594
score: 53.636177
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 216..593

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG11g10480.1Cp4.1LG11g10480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034314 Arp2/3 complex-mediated actin nucleation
biological_process GO:0016579 protein deubiquitination
biological_process GO:0030833 regulation of actin filament polymerization
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005885 Arp2/3 protein complex
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004843 thiol-dependent deubiquitinase
molecular_function GO:0008270 zinc ion binding