Cp4.1LG11g10360 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG11g10360
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionRING-type domain-containing protein
LocationCp4.1LG11: 8646596 .. 8648573 (-)
RNA-Seq ExpressionCp4.1LG11g10360
SyntenyCp4.1LG11g10360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATGGTCTCTATACACTCTCCGTTTTACTTTTGAGTGACTACATTTCTTGTTGTTGTACGCTCTCTCGCCTTCAATGGCATGGACCCGCTTGTGTTTGTTACCCTCATCACTCCCCAGCTTCTTGCCCTTCTTACTTTATCCTTATTTTAGTTCCTCCCACTCCCACTTCCCTATCTTCGATTTCACCAGATTTTATTCGCTTGTTTCTTCATGTTCTTCATGTTCCTCGTTTTTTTTCTTCTGGGTTTTCTGTTTATTTGCTCCAAACCACGGATTTCGTTGGTTTCGAGTTGTAATCCTTAATGGGGCTTGGTTTCTCTGTTTCTCTCTGTCTCAGTTCATCTGGGTTTTGGTTGATTTTGATGAAACTCTGAAATTTGGTCGATTTTGACTTGAAATCGGTGCGGATTTGAGATTTTTTGCTGGGTTTCTTCGTTTTCTTCTCGTTTTTCTGTTTTGATTCATTTGCTGAAACTGAGAAACTCCATTGATTCTGAGTTGAACTCTGTTTCTTCTTCTTCTTCTGCGATTGGGGTCTGATTCAAATGCAGAAAAAAACTGAGAAACATGATGGATTTTGAGTTGAAATTAGTGTGGATTTGAGAATTTTTGCTTGGATTCTTCGTTTTCTTCTCGTTTTTTCTGTAATGGGTATTTCTGATTCATTTGCTGAAACTGAGAAACTCCATTGATTCTGAGTTGAACTCTGTTTCTTCTTGTTCTTCTGCGATTGGGTTCTGATTCAAATACAGAAAAAAACTGAGAAACTTGATGGATTTAGAATTGAAATTGGTGTAGATTTGAGAATTTTTTCTGGGTTTCTTCGTTTTCTTCTCGTTTTTCTGTAATGGATATTTCTGATTCAAATGCTGATAAGAGCAGAGAAGCTTGATGGATTTTGAGTAGAAATCCTCTTGGGTTTAATCGAGCAAGTTTTCCCCTCTGTTCTTCAGTTCTGATAAAAGTTCTGCAGATTTTGAATTGAAATCATAGTGGGGGTTGATTCTCAGTTTCAATCCCAGCAAGTTTTCCCAAATCAATGGCCGGAATGTTGCCGGGAGTTGAATGCGCCAGAAGGCGGCGGTTCCACCAGAGCATAACCTCATCGGACTCGCCGTCCACGGCGGCGAAATTAGGATCCACAAGAAGGCCGTCGTTCTGTTTGTACACGAGCAACCGTGATTTTCACATCACTTCATCATCATCATCTTCTTCACAGGTATGAATGAATGAATGAAATCTAAGAATTTTTCTGTTTGTTTCCCGGGAAAATTCTGATGAATGTTGAACATAAAATTAAGCAACGGAGCTCATCCAATCAAGCTTACCAGAACGAGAAGCTTGGTGAAATTGCAAGAGAAGCTAAGGAACGGTTGGATGAGAGGTTAAGAACTCATAGAAAACCTGAAAATCCAAGGTAATTTCTTCTTAATTTTACTTTATGATCAAATTTGATCTTTCGAACTCTTAAAACTGTTAAGAACGAAGTCGAAACCGTTCAAATGAAAGGAACTAAATTTGTAATTTAACCTAAAATCAACGTTAAAATCGAAAATAATCGTACAGAAAAAAGAGTGGAGAAGGGTCGAAAGAAGAATCAATAACGAAGAAGAGGTTCACTTGGGGGAAGCTGATGAAATGGAAAGCCTCAGAGCAAGAAGAGTGCACCATTTGTTTGGAAGGGTTCAGAGCGGGCGAGCCACTGGTCCATCTTCCATGCGCCCACAAGTTCCACTCTGCGTGCTTGGTGCCTTGGCTCCAAGCCAATGCCCATTGCCCTTGTTGCAGGTTCCCGTTTCCCTCTTAATAACAAATCAATCACCGAGCTTGCAATTTTATATTGTTAGATGTACGAGAGAAGTGAAATAAGAAAGGATTTAGCCTCGACATTGCCGTCATGTGATCTTGTTTTCAAGACTTTGTTTGTTATATTATATAATCGAAGTTCGGTTTGTTGTCTTCAAGTTCACTTGAATC

mRNA sequence

CATGGTCTCTATACACTCTCCGTTTTACTTTTGAGTGACTACATTTCTTGTTGTTGTACGCTCTCTCGCCTTCAATGGCATGGACCCGCTTGTGTTTGTTACCCTCATCACTCCCCAGCTTCTTGCCCTTCTTACTTTATCCTTATTTTAGTTCCTCCCACTCCCACTTCCCTATCTTCGATTTCACCAGATTTTATTCGCTTGTTTCTTCATGTTCTTCATGTTCCTCGTTTTTTTTCTTCTGGGTTTTCTGTTTATTTGCTCCAAACCACGGATTTCGTTGGTTTCGAGTTGTAATCCTTAATGGGGCTTGGTTTCTCTGTTTCTCTCTGTCTCAGTTCATCTGGGTTTTGGTTGATTTTGATGAAACTCTGAAATTTGGTCGATTTTGACTTGAAATCGGTGCGGATTTGAGATTTTTTGCTGGGTTTCTTCGTTTTCTTCTCGTTTTTCTGTTTTGATTCATTTGCTGAAACTGAGAAACTCCATTGATTCTGAGTTGAACTCTGTTTCTTCTTCTTCTTCTGCGATTGGGGTCTGATTCAAATGCAGAAAAAAACTGAGAAACATGATGGATTTTGAGTTGAAATTAGTGTGGATTTGAGAATTTTTGCTTGGATTCTTCGTTTTCTTCTCGTTTTTTCTGTAATGGGTATTTCTGATTCATTTGCTGAAACTGAGAAACTCCATTGATTCTGAGTTGAACTCTGTTTCTTCTTGTTCTTCTGCGATTGGGTTCTGATTCAAATACAGAAAAAAACTGAGAAACTTGATGGATTTAGAATTGAAATTGGTGTAGATTTGAGAATTTTTTCTGGGTTTCTTCGTTTTCTTCTCGTTTTTCTGTAATGGATATTTCTGATTCAAATGCTGATAAGAGCAGAGAAGCTTGATGGATTTTGAGTAGAAATCCTCTTGGGTTTAATCGAGCAAGTTTTCCCCTCTGTTCTTCAGTTCTGATAAAAGTTCTGCAGATTTTGAATTGAAATCATAGTGGGGGTTGATTCTCAGTTTCAATCCCAGCAAGTTTTCCCAAATCAATGGCCGGAATGTTGCCGGGAGTTGAATGCGCCAGAAGGCGGCGGTTCCACCAGAGCATAACCTCATCGGACTCGCCGTCCACGGCGGCGAAATTAGGATCCACAAGAAGGCCGTCGTTCTGTTTGTACACGAGCAACCGTGATTTTCACATCACTTCATCATCATCATCTTCTTCACAGCAACGGAGCTCATCCAATCAAGCTTACCAGAACGAGAAGCTTGGTGAAATTGCAAGAGAAGCTAAGGAACGGTTGGATGAGAGGTTAAGAACTCATAGAAAACCTGAAAATCCAAGAAAAAAGAGTGGAGAAGGGTCGAAAGAAGAATCAATAACGAAGAAGAGGTTCACTTGGGGGAAGCTGATGAAATGGAAAGCCTCAGAGCAAGAAGAGTGCACCATTTGTTTGGAAGGGTTCAGAGCGGGCGAGCCACTGGTCCATCTTCCATGCGCCCACAAGTTCCACTCTGCGTGCTTGGTGCCTTGGCTCCAAGCCAATGCCCATTGCCCTTGTTGCAGGTTCCCGTTTCCCTCTTAATAACAAATCAATCACCGAGCTTGCAATTTTATATTGTTAGATGTACGAGAGAAGTGAAATAAGAAAGGATTTAGCCTCGACATTGCCGTCATGTGATCTTGTTTTCAAGACTTTGTTTGTTATATTATATAATCGAAGTTCGGTTTGTTGTCTTCAAGTTCACTTGAATC

Coding sequence (CDS)

ATGGCCGGAATGTTGCCGGGAGTTGAATGCGCCAGAAGGCGGCGGTTCCACCAGAGCATAACCTCATCGGACTCGCCGTCCACGGCGGCGAAATTAGGATCCACAAGAAGGCCGTCGTTCTGTTTGTACACGAGCAACCGTGATTTTCACATCACTTCATCATCATCATCTTCTTCACAGCAACGGAGCTCATCCAATCAAGCTTACCAGAACGAGAAGCTTGGTGAAATTGCAAGAGAAGCTAAGGAACGGTTGGATGAGAGGTTAAGAACTCATAGAAAACCTGAAAATCCAAGAAAAAAGAGTGGAGAAGGGTCGAAAGAAGAATCAATAACGAAGAAGAGGTTCACTTGGGGGAAGCTGATGAAATGGAAAGCCTCAGAGCAAGAAGAGTGCACCATTTGTTTGGAAGGGTTCAGAGCGGGCGAGCCACTGGTCCATCTTCCATGCGCCCACAAGTTCCACTCTGCGTGCTTGGTGCCTTGGCTCCAAGCCAATGCCCATTGCCCTTGTTGCAGGTTCCCGTTTCCCTCTTAA

Protein sequence

MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSSSSQQRSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGSKEESITKKRFTWGKLMKWKASEQEECTICLEGFRAGEPLVHLPCAHKFHSACLVPWLQANAHCPCCRFPFPS
Homology
BLAST of Cp4.1LG11g10360 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 5.4e-10
Identity = 30/90 (33.33%), Postives = 46/90 (51.11%), Query Frame = 0

Query: 89  LRTHRKPENPRKKSGEGSKEESITKKRFTWGKLMKWKASEQEECTICLEGFRAGEPLVHL 148
           L  H    +P +     +K+E++         L   K  E   C++CL+    G     +
Sbjct: 163 LLQHLAESDPSRNGTPPAKKEAVE-------ALPTVKIEEVVSCSVCLDDLEVGSQAKQM 222

Query: 149 PCAHKFHSACLVPWLQANAHCPCCRFPFPS 179
           PC HKFHS+C++PWL+ ++ CP CRF  PS
Sbjct: 223 PCEHKFHSSCILPWLELHSSCPVCRFELPS 245

BLAST of Cp4.1LG11g10360 vs. ExPASy Swiss-Prot
Match: Q852U6 (Probable E3 ubiquitin-protein ligase RHY1A OS=Arabidopsis thaliana OX=3702 GN=RHY1A PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 7.1e-10
Identity = 53/166 (31.93%), Postives = 80/166 (48.19%), Query Frame = 0

Query: 21  TSSDSPSTAAKLGSTRRPSFCLYTSNRDFH----ITSSSSSSS------QQRSSSNQAYQ 80
           T + S S     GS R P   L   +R F     ++ SS+S S       QR SS  +  
Sbjct: 96  TETQSSSFVNLNGSERLPGAVLLARDRLFERLRGVSLSSNSRSNRVSLDDQRESSFHSID 155

Query: 81  NEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGSKEESIT---KKRFTWGKLMKWKAS 140
            + + ++A      L      ++KP        +G  +++I    ++ F+  ++     S
Sbjct: 156 GDPIFQLA-----GLQVTYECNKKP--------QGLTQDAINCLHRQTFSSAEV----KS 215

Query: 141 EQEECTICLEGFRAGEPLVHLPCAHKFHSACLVPWLQANAHCPCCR 174
           E  +C+ICLE F  G+ L+ LPC H FHS+CL PWL+A   CPCCR
Sbjct: 216 EMRDCSICLESFTKGDMLISLPCTHSFHSSCLNPWLRACGDCPCCR 244

BLAST of Cp4.1LG11g10360 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.2e-09
Identity = 22/52 (42.31%), Postives = 32/52 (61.54%), Query Frame = 0

Query: 127 SEQEECTICLEGFRAGEPLVHLPCAHKFHSACLVPWLQANAHCPCCRFPFPS 179
           SE  +C +C++ F  G  +  +PC H FH  CL+PWL+ +  CP CRF  P+
Sbjct: 211 SEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPT 262

BLAST of Cp4.1LG11g10360 vs. ExPASy Swiss-Prot
Match: Q9C1X4 (Uncharacterized RING finger protein P32A8.03c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAP32A8.03c PE=4 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 2.7e-09
Identity = 23/48 (47.92%), Postives = 31/48 (64.58%), Query Frame = 0

Query: 128 EQEECTICLEGFRAGEPLVHLPCAHKFHSACLVPWLQANAHCPCCRFP 176
           E+ ECTIC+E F+  + ++ LPC H FH  C+ PWL+ N  C  CR P
Sbjct: 392 EEGECTICMEMFKINDDVIQLPCKHYFHENCIKPWLRVNGTCAICRAP 439

BLAST of Cp4.1LG11g10360 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 5.1e-08
Identity = 23/50 (46.00%), Postives = 27/50 (54.00%), Query Frame = 0

Query: 129 QEECTICLEGFRAGEPLVHLPCAHKFHSACLVPWLQANAHCPCCRFPFPS 179
           +  C +C E F AG     +PC H FH  C+VPWL     CP CRF  PS
Sbjct: 212 EANCAVCTEVFEAGIEGREMPCKHIFHGDCIVPWLSIRNSCPVCRFELPS 261

BLAST of Cp4.1LG11g10360 vs. NCBI nr
Match: XP_023545449.1 (probable E3 ubiquitin-protein ligase RHY1A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 361 bits (926), Expect = 8.63e-126
Identity = 178/178 (100.00%), Postives = 178/178 (100.00%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSSSSQ 60
           MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSSSSQ
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSSSSQ 60

Query: 61  QRSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGSKEESITKKRFTWGK 120
           QRSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGSKEESITKKRFTWGK
Sbjct: 61  QRSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGSKEESITKKRFTWGK 120

Query: 121 LMKWKASEQEECTICLEGFRAGEPLVHLPCAHKFHSACLVPWLQANAHCPCCRFPFPS 178
           LMKWKASEQEECTICLEGFRAGEPLVHLPCAHKFHSACLVPWLQANAHCPCCRFPFPS
Sbjct: 121 LMKWKASEQEECTICLEGFRAGEPLVHLPCAHKFHSACLVPWLQANAHCPCCRFPFPS 178

BLAST of Cp4.1LG11g10360 vs. NCBI nr
Match: XP_022946687.1 (probable E3 ubiquitin-protein ligase RHY1A [Cucurbita moschata])

HSP 1 Score: 343 bits (881), Expect = 6.07e-119
Identity = 170/178 (95.51%), Postives = 173/178 (97.19%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSSSSQ 60
           MAGMLPGVECARRRRFHQSITSSD+PSTAAKLGSTRRPSFCLYTSNRDFHITSSSS S Q
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCS-Q 60

Query: 61  QRSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGSKEESITKKRFTWGK 120
           QRSSS+QAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEG KEESITKKRFTWGK
Sbjct: 61  QRSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGWKEESITKKRFTWGK 120

Query: 121 LMKWKASEQEECTICLEGFRAGEPLVHLPCAHKFHSACLVPWLQANAHCPCCRFPFPS 178
           LMKWKASEQEEC +CLEGFRAGEPLVHLPCAHKFHSACLVPWLQ NAHCPCCRFPFPS
Sbjct: 121 LMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAHCPCCRFPFPS 177

BLAST of Cp4.1LG11g10360 vs. NCBI nr
Match: XP_022999563.1 (probable E3 ubiquitin-protein ligase RHY1A [Cucurbita maxima])

HSP 1 Score: 333 bits (854), Expect = 8.23e-115
Identity = 165/175 (94.29%), Postives = 170/175 (97.14%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSSS-S 60
           MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSSS S
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSSSRS 60

Query: 61  QQRSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGSKEESITKKRFTWG 120
           QQRSSSNQAYQ EKLGEIAREAKERLDERL++HRKP+NPRKKSGEGSKEESITKKRFTWG
Sbjct: 61  QQRSSSNQAYQTEKLGEIAREAKERLDERLKSHRKPQNPRKKSGEGSKEESITKKRFTWG 120

Query: 121 KLMKWKASEQEECTICLEGFRAGEPLVHLPCAHKFHSACLVPWLQANAHCPCCRF 174
           KLMKWKASEQ+EC +CLEGFRAGEPLVHLPCAHKFHSAC VPWLQAN HCPCCRF
Sbjct: 121 KLMKWKASEQQECAVCLEGFRAGEPLVHLPCAHKFHSACFVPWLQANHHCPCCRF 175

BLAST of Cp4.1LG11g10360 vs. NCBI nr
Match: KAG7030344.1 (putative E3 ubiquitin-protein ligase RHY1A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 333 bits (855), Expect = 8.52e-115
Identity = 169/190 (88.95%), Postives = 172/190 (90.53%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSSSSQ 60
           MAGMLPGVECARRRRFHQSIT SD+PSTAAKLGSTRRPSFCLYTSNRDFHITSSSS S Q
Sbjct: 1   MAGMLPGVECARRRRFHQSITLSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCS-Q 60

Query: 61  QRSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEG------------SKE 120
           QRSSS+QAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEG             KE
Sbjct: 61  QRSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGLKNSLEKKEKAKGKE 120

Query: 121 ESITKKRFTWGKLMKWKASEQEECTICLEGFRAGEPLVHLPCAHKFHSACLVPWLQANAH 178
           ESITKKRFTWGKLMKWKASEQEEC +CLEGFRAGEPLVHLPCAHKFHSACLVPWLQ NAH
Sbjct: 121 ESITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAH 180

BLAST of Cp4.1LG11g10360 vs. NCBI nr
Match: XP_038891102.1 (probable E3 ubiquitin-protein ligase RHY1A [Benincasa hispida])

HSP 1 Score: 302 bits (774), Expect = 2.49e-102
Identity = 160/199 (80.40%), Postives = 164/199 (82.41%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSSSSQ 60
           MAGMLPGVECARRRRFHQSI SSDSPSTAAK GSTRRPSFCLYTSNRDFH TSSSSS  Q
Sbjct: 1   MAGMLPGVECARRRRFHQSIASSDSPSTAAKSGSTRRPSFCLYTSNRDFHSTSSSSS--Q 60

Query: 61  QRSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKS--------------GEGS 120
            RSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPEN R+KS              GE  
Sbjct: 61  HRSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPENSRRKSENEKVGWKGSSEKNGEEK 120

Query: 121 -----KEESI--TKKRFTWGKLMKWKASEQEECTICLEGFRAGEPLVHLPCAHKFHSACL 178
                KEESI  TKKRF WGKLMKW+ASEQEECT+CLE FR GEPLVHLPCAHKFHS CL
Sbjct: 121 ERTKGKEESIIITKKRFNWGKLMKWRASEQEECTVCLERFRVGEPLVHLPCAHKFHSTCL 180

BLAST of Cp4.1LG11g10360 vs. ExPASy TrEMBL
Match: A0A6J1G4D6 (probable E3 ubiquitin-protein ligase RHY1A OS=Cucurbita moschata OX=3662 GN=LOC111450678 PE=4 SV=1)

HSP 1 Score: 343 bits (881), Expect = 2.94e-119
Identity = 170/178 (95.51%), Postives = 173/178 (97.19%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSSSSQ 60
           MAGMLPGVECARRRRFHQSITSSD+PSTAAKLGSTRRPSFCLYTSNRDFHITSSSS S Q
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCS-Q 60

Query: 61  QRSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGSKEESITKKRFTWGK 120
           QRSSS+QAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEG KEESITKKRFTWGK
Sbjct: 61  QRSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGWKEESITKKRFTWGK 120

Query: 121 LMKWKASEQEECTICLEGFRAGEPLVHLPCAHKFHSACLVPWLQANAHCPCCRFPFPS 178
           LMKWKASEQEEC +CLEGFRAGEPLVHLPCAHKFHSACLVPWLQ NAHCPCCRFPFPS
Sbjct: 121 LMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAHCPCCRFPFPS 177

BLAST of Cp4.1LG11g10360 vs. ExPASy TrEMBL
Match: A0A6J1KK21 (probable E3 ubiquitin-protein ligase RHY1A OS=Cucurbita maxima OX=3661 GN=LOC111493891 PE=4 SV=1)

HSP 1 Score: 333 bits (854), Expect = 3.98e-115
Identity = 165/175 (94.29%), Postives = 170/175 (97.14%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSSS-S 60
           MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSSS S
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSSSRS 60

Query: 61  QQRSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGSKEESITKKRFTWG 120
           QQRSSSNQAYQ EKLGEIAREAKERLDERL++HRKP+NPRKKSGEGSKEESITKKRFTWG
Sbjct: 61  QQRSSSNQAYQTEKLGEIAREAKERLDERLKSHRKPQNPRKKSGEGSKEESITKKRFTWG 120

Query: 121 KLMKWKASEQEECTICLEGFRAGEPLVHLPCAHKFHSACLVPWLQANAHCPCCRF 174
           KLMKWKASEQ+EC +CLEGFRAGEPLVHLPCAHKFHSAC VPWLQAN HCPCCRF
Sbjct: 121 KLMKWKASEQQECAVCLEGFRAGEPLVHLPCAHKFHSACFVPWLQANHHCPCCRF 175

BLAST of Cp4.1LG11g10360 vs. ExPASy TrEMBL
Match: A0A6J1HQI5 (probable E3 ubiquitin-protein ligase RHY1A isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465688 PE=4 SV=1)

HSP 1 Score: 294 bits (752), Expect = 1.85e-99
Identity = 150/185 (81.08%), Postives = 157/185 (84.86%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSSSSQ 60
           MAGMLPGVECARRRRFHQSITSS+S STAAKLGSTRR SFCLYTSNRDFH+TSSSS   Q
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSSS---Q 60

Query: 61  QRSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGS----------KEES 120
           QR SSN AYQNEKLG+ AREAKERLDERLRTHRKPEN R KS              KEE+
Sbjct: 61  QRCSSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLKKENVKGKEET 120

Query: 121 ITKKRFTWGKLMKWKASEQEECTICLEGFRAGEPLVHLPCAHKFHSACLVPWLQANAHCP 175
            TKKRF+WGKLMKWKASEQEECT+CLE FRA EPLVHLPCAHKFHS CLVPWLQANAHCP
Sbjct: 121 TTKKRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCP 180

BLAST of Cp4.1LG11g10360 vs. ExPASy TrEMBL
Match: A0A6J1FHS8 (E3 ubiquitin-protein ligase ZNRF3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444265 PE=4 SV=1)

HSP 1 Score: 291 bits (746), Expect = 1.52e-98
Identity = 150/185 (81.08%), Postives = 157/185 (84.86%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSSSSQ 60
           MAGMLPGVECARRRRFHQSITSSDS STAAKLGSTRR SFCLY SNRDFH+TSSSS   Q
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSASTAAKLGSTRRSSFCLYASNRDFHLTSSSS---Q 60

Query: 61  QRSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGS----------KEES 120
           QR SSN AYQ+EKLG+ AREAKERLDERLRTHRKPEN RKKS              KEE+
Sbjct: 61  QRCSSNHAYQDEKLGQAAREAKERLDERLRTHRKPENSRKKSSSEEVGLKKEKAKGKEET 120

Query: 121 ITKKRFTWGKLMKWKASEQEECTICLEGFRAGEPLVHLPCAHKFHSACLVPWLQANAHCP 175
            TKKRF WGKLMKWKASEQEECT+CLE FRA EPLVHLPCAHKFHSACLVPWLQANA+CP
Sbjct: 121 TTKKRFGWGKLMKWKASEQEECTVCLEKFRATEPLVHLPCAHKFHSACLVPWLQANAYCP 180

BLAST of Cp4.1LG11g10360 vs. ExPASy TrEMBL
Match: A0A0A0LG47 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G009360 PE=4 SV=1)

HSP 1 Score: 282 bits (721), Expect = 1.64e-94
Identity = 149/197 (75.63%), Postives = 159/197 (80.71%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSSSSQ 60
           MAGMLPGVECARRRRFHQSI SSDSP TA K GSTRRPSFCLYTSNRDFH+ SSSSS  Q
Sbjct: 1   MAGMLPGVECARRRRFHQSINSSDSPFTAGKSGSTRRPSFCLYTSNRDFHLISSSSS--Q 60

Query: 61  QRSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKS------GEGSKE------ 120
           QRS +NQAYQN+KLGEIAREAKERLDERLRTHRKPEN RKK+      G+ S+E      
Sbjct: 61  QRSLTNQAYQNDKLGEIAREAKERLDERLRTHRKPENSRKKNNNEEVKGKESREKNNGEK 120

Query: 121 ---------ESITKKRFTWG-KLMKWKASEQEECTICLEGFRAGEPLVHLPCAHKFHSAC 175
                    E  TKKRF+WG KLMKWKASEQEEC++CLE FR  EPL+HLPCAHKFHS C
Sbjct: 121 KEMKNTKGKEEETKKRFSWGRKLMKWKASEQEECSVCLERFRLSEPLLHLPCAHKFHSTC 180

BLAST of Cp4.1LG11g10360 vs. TAIR 10
Match: AT2G15580.1 (RING/U-box superfamily protein )

HSP 1 Score: 145.6 bits (366), Expect = 3.8e-35
Identity = 89/191 (46.60%), Postives = 112/191 (58.64%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFH---QSITSSDSPSTAAKLGS--TRRPSFCLYTSNRDFHITSSS 60
           MAGMLPGVECARRRRFH     I SS++ S AA  G   TRRPSF LYT+N + H    S
Sbjct: 1   MAGMLPGVECARRRRFHGGAPPIESSNTASVAAAAGHVWTRRPSFSLYTTNHESHQAHVS 60

Query: 61  SSSSQQRSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGE----------G 120
            S    R+ S     +EKL   A+EAK+RL++RLR   +     K  G           G
Sbjct: 61  FSERSVRNKSYGEDNDEKLDGAAKEAKQRLNKRLRIPPRTRQNGKDKGNKLEQGKGKPLG 120

Query: 121 SKEESITKKRFTWGKLMKW---KASEQEECTICLEGFRAGEPLVHLPCAHKFHSACLVPW 174
                +   + + G+LM+W   +  EQ++C ICL+ F+ GE LVHLPCAHKFHS CL+PW
Sbjct: 121 DLPTEVVGLKKSRGRLMEWFKRRVREQQDCAICLDRFKKGETLVHLPCAHKFHSICLLPW 180

BLAST of Cp4.1LG11g10360 vs. TAIR 10
Match: AT2G15580.2 (RING/U-box superfamily protein )

HSP 1 Score: 75.1 bits (183), Expect = 6.4e-14
Identity = 57/116 (49.14%), Postives = 69/116 (59.48%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFH---QSITSSDSPSTAAKLGS--TRRPSFCLYTSNRDFHITSSS 60
           MAGMLPGVECARRRRFH     I SS++ S AA  G   TRRPSF LYT+N + H    S
Sbjct: 1   MAGMLPGVECARRRRFHGGAPPIESSNTASVAAAAGHVWTRRPSFSLYTTNHESHQAHVS 60

Query: 61  SSSSQQRSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGSKEESI 112
            S    R+ S     +EKL   A+EAK+RL++RLR       PR  SG+  K + I
Sbjct: 61  FSERSVRNKSYGEDNDEKLDGAAKEAKQRLNKRLRI-----PPRTSSGKMVKTKGI 111

BLAST of Cp4.1LG11g10360 vs. TAIR 10
Match: AT4G26400.2 (RING/U-box superfamily protein )

HSP 1 Score: 67.8 bits (164), Expect = 1.0e-11
Identity = 25/53 (47.17%), Postives = 34/53 (64.15%), Query Frame = 0

Query: 125 KASEQEECTICLEGFRAGEPLVHLPCAHKFHSACLVPWLQANAHCPCCRFPFP 178
           K SE  +C+ICL+ F  G     +PC HKFH  C+VPWL+ ++ CP CR+  P
Sbjct: 234 KISESLQCSICLDDFDKGSEAKEMPCKHKFHIRCIVPWLELHSSCPVCRYELP 286

BLAST of Cp4.1LG11g10360 vs. TAIR 10
Match: AT4G26400.1 (RING/U-box superfamily protein )

HSP 1 Score: 67.8 bits (164), Expect = 1.0e-11
Identity = 25/53 (47.17%), Postives = 34/53 (64.15%), Query Frame = 0

Query: 125 KASEQEECTICLEGFRAGEPLVHLPCAHKFHSACLVPWLQANAHCPCCRFPFP 178
           K SE  +C+ICL+ F  G     +PC HKFH  C+VPWL+ ++ CP CR+  P
Sbjct: 234 KISESLQCSICLDDFDKGSEAKEMPCKHKFHIRCIVPWLELHSSCPVCRYELP 286

BLAST of Cp4.1LG11g10360 vs. TAIR 10
Match: AT1G49850.1 (RING/U-box superfamily protein )

HSP 1 Score: 65.5 bits (158), Expect = 5.0e-11
Identity = 53/166 (31.93%), Postives = 80/166 (48.19%), Query Frame = 0

Query: 21  TSSDSPSTAAKLGSTRRPSFCLYTSNRDFH----ITSSSSSSS------QQRSSSNQAYQ 80
           T + S S     GS R P   L   +R F     ++ SS+S S       QR SS  +  
Sbjct: 96  TETQSSSFVNLNGSERLPGAVLLARDRLFERLRGVSLSSNSRSNRVSLDDQRESSFHSID 155

Query: 81  NEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGSKEESIT---KKRFTWGKLMKWKAS 140
            + + ++A      L      ++KP        +G  +++I    ++ F+  ++     S
Sbjct: 156 GDPIFQLA-----GLQVTYECNKKP--------QGLTQDAINCLHRQTFSSAEV----KS 215

Query: 141 EQEECTICLEGFRAGEPLVHLPCAHKFHSACLVPWLQANAHCPCCR 174
           E  +C+ICLE F  G+ L+ LPC H FHS+CL PWL+A   CPCCR
Sbjct: 216 EMRDCSICLESFTKGDMLISLPCTHSFHSSCLNPWLRACGDCPCCR 244

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6AVN25.4e-1033.33E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI... [more]
Q852U67.1e-1031.93Probable E3 ubiquitin-protein ligase RHY1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q8LPN71.2e-0942.31E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
Q9C1X42.7e-0947.92Uncharacterized RING finger protein P32A8.03c OS=Schizosaccharomyces pombe (stra... [more]
Q9SNB65.1e-0846.00E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ... [more]
Match NameE-valueIdentityDescription
XP_023545449.18.63e-126100.00probable E3 ubiquitin-protein ligase RHY1A [Cucurbita pepo subsp. pepo][more]
XP_022946687.16.07e-11995.51probable E3 ubiquitin-protein ligase RHY1A [Cucurbita moschata][more]
XP_022999563.18.23e-11594.29probable E3 ubiquitin-protein ligase RHY1A [Cucurbita maxima][more]
KAG7030344.18.52e-11588.95putative E3 ubiquitin-protein ligase RHY1A, partial [Cucurbita argyrosperma subs... [more]
XP_038891102.12.49e-10280.40probable E3 ubiquitin-protein ligase RHY1A [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1G4D62.94e-11995.51probable E3 ubiquitin-protein ligase RHY1A OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1KK213.98e-11594.29probable E3 ubiquitin-protein ligase RHY1A OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1HQI51.85e-9981.08probable E3 ubiquitin-protein ligase RHY1A isoform X1 OS=Cucurbita maxima OX=366... [more]
A0A6J1FHS81.52e-9881.08E3 ubiquitin-protein ligase ZNRF3-like isoform X1 OS=Cucurbita moschata OX=3662 ... [more]
A0A0A0LG471.64e-9475.63RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G009360 P... [more]
Match NameE-valueIdentityDescription
AT2G15580.13.8e-3546.60RING/U-box superfamily protein [more]
AT2G15580.26.4e-1449.14RING/U-box superfamily protein [more]
AT4G26400.21.0e-1147.17RING/U-box superfamily protein [more]
AT4G26400.11.0e-1147.17RING/U-box superfamily protein [more]
AT1G49850.15.0e-1131.93RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 132..172
e-value: 3.4E-5
score: 33.3
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 131..173
e-value: 1.5E-11
score: 44.4
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 132..173
score: 12.356501
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 89..178
e-value: 1.6E-18
score: 68.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 16..36
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 51..72
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 20..36
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 51..110
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 75..110
NoneNo IPR availablePANTHERPTHR22765RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAININGcoord: 117..173
coord: 1..99
NoneNo IPR availablePANTHERPTHR22765:SF204RING/U-BOX SUPERFAMILY PROTEINcoord: 117..173
coord: 1..99
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 131..173
e-value: 2.16376E-17
score: 70.0233
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 117..176

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG11g10360.1Cp4.1LG11g10360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
molecular_function GO:0061630 ubiquitin protein ligase activity