Cp4.1LG11g10020 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG11g10020
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionRibonuclease T(2)
LocationCp4.1LG11: 8454388 .. 8455266 (+)
RNA-Seq ExpressionCp4.1LG11g10020
SyntenyCp4.1LG11g10020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTACCTCTCAACTCCCTCCCCTTCTCCTTCTCCTTCTCCATTTCCTTCAACCTCATGGGAAATTTCGTAGTTACGTTTTTCCTCTTTTATTTAGCTTTTTCATCTGTACAAGCTAAATTCCAAGAGGTCGAGCGACCTAAACCTAAACCTAAGTATCAATATGAATACTTTCAAATGGTACAGCAATGGCCGCAGTCCATTTGTAATAGTGAGCATTGTATAGGTAAGCCTAAAGCTATGTTCACCATTCATGGGCTCTGGCCTCAAAATTACTCTACTTCACTTATTTGCGACGGGTCTCCGTTCGCCGACGAATATGTAAGTTTTTTTTTAATAATAATAATATTATTATTATTACCGTCTAGGTATAGATGAGTATTTGATGCTTTTTATATATATATTTTTTCTTTACTCAGATCAAATCAATTAGAGAGCAGCTAAATCTATATTGGCCAACCCTATTGTTTAAAGATAACAAATCATTTTGGAATCATGAATGGATGATTCACGGTGAGTGTTCAGACCCACCCTTTAACCCATTTCAGTATTTTCAGAAAACTCTGAAAATCCGAAAAACCTACGATTTATTAGCCATCCTCAATGCTGCGGGACTTGGACCAACTACTGCTGCCCTTCGTAACCGTGATGACATAGAAAAAGCAATTGGAGCTGCAATAGGACAAAAGCCAGTTCTAAAATGCAACACTAACACCAGGACAAGAGACCGCCAACTATCGGAAGTTGTTTTATGCCTTGATGAAGATGGTGTGAAGTTAATTGATTGTGCTAAATTTGTTAGCACCAATTGCCCTCTTACTTTTTCATGGCCCCCTTTTGTTGCTAGTGAGTAACTGGTTCAGAAGTCATCCGCTCCCAAAT

mRNA sequence

TTACCTCTCAACTCCCTCCCCTTCTCCTTCTCCTTCTCCATTTCCTTCAACCTCATGGGAAATTTCGTAGTTACGTTTTTCCTCTTTTATTTAGCTTTTTCATCTGTACAAGCTAAATTCCAAGAGGTCGAGCGACCTAAACCTAAACCTAAGTATCAATATGAATACTTTCAAATGGTACAGCAATGGCCGCAGTCCATTTGTAATAGTGAGCATTGTATAGGTAAGCCTAAAGCTATGTTCACCATTCATGGGCTCTGGCCTCAAAATTACTCTACTTCACTTATTTGCGACGGGTCTCCGTTCGCCGACGAATATATCAAATCAATTAGAGAGCAGCTAAATCTATATTGGCCAACCCTATTGTTTAAAGATAACAAATCATTTTGGAATCATGAATGGATGATTCACGGTGAGTGTTCAGACCCACCCTTTAACCCATTTCAGTATTTTCAGAAAACTCTGAAAATCCGAAAAACCTACGATTTATTAGCCATCCTCAATGCTGCGGGACTTGGACCAACTACTGCTGCCCTTCGTAACCGTGATGACATAGAAAAAGCAATTGGAGCTGCAATAGGACAAAAGCCAGTTCTAAAATGCAACACTAACACCAGGACAAGAGACCGCCAACTATCGGAAGTTGTTTTATGCCTTGATGAAGATGGTGTGAAGTTAATTGATTGTGCTAAATTTGTTAGCACCAATTGCCCTCTTACTTTTTCATGGCCCCCTTTTGTTGCTAGTGAGTAACTGGTTCAGAAGTCATCCGCTCCCAAAT

Coding sequence (CDS)

ATGGGAAATTTCGTAGTTACGTTTTTCCTCTTTTATTTAGCTTTTTCATCTGTACAAGCTAAATTCCAAGAGGTCGAGCGACCTAAACCTAAACCTAAGTATCAATATGAATACTTTCAAATGGTACAGCAATGGCCGCAGTCCATTTGTAATAGTGAGCATTGTATAGGTAAGCCTAAAGCTATGTTCACCATTCATGGGCTCTGGCCTCAAAATTACTCTACTTCACTTATTTGCGACGGGTCTCCGTTCGCCGACGAATATATCAAATCAATTAGAGAGCAGCTAAATCTATATTGGCCAACCCTATTGTTTAAAGATAACAAATCATTTTGGAATCATGAATGGATGATTCACGGTGAGTGTTCAGACCCACCCTTTAACCCATTTCAGTATTTTCAGAAAACTCTGAAAATCCGAAAAACCTACGATTTATTAGCCATCCTCAATGCTGCGGGACTTGGACCAACTACTGCTGCCCTTCGTAACCGTGATGACATAGAAAAAGCAATTGGAGCTGCAATAGGACAAAAGCCAGTTCTAAAATGCAACACTAACACCAGGACAAGAGACCGCCAACTATCGGAAGTTGTTTTATGCCTTGATGAAGATGGTGTGAAGTTAATTGATTGTGCTAAATTTGTTAGCACCAATTGCCCTCTTACTTTTTCATGGCCCCCTTTTGTTGCTAGTGAGTAA

Protein sequence

MGNFVVTFFLFYLAFSSVQAKFQEVERPKPKPKYQYEYFQMVQQWPQSICNSEHCIGKPKAMFTIHGLWPQNYSTSLICDGSPFADEYIKSIREQLNLYWPTLLFKDNKSFWNHEWMIHGECSDPPFNPFQYFQKTLKIRKTYDLLAILNAAGLGPTTAALRNRDDIEKAIGAAIGQKPVLKCNTNTRTRDRQLSEVVLCLDEDGVKLIDCAKFVSTNCPLTFSWPPFVASE
Homology
BLAST of Cp4.1LG11g10020 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.1e-34
Identity = 73/183 (39.89%), Postives = 102/183 (55.74%), Query Frame = 0

Query: 36  YEYFQMVQQWPQSICN---SEHCIGKPKAMFTIHGLWPQNYSTSLI-C-DGSPFADEYIK 95
           ++ F  VQQWP ++C+   S  C G     FTIHGLWPQ   TSL  C  GSPF    I 
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKIS 60

Query: 96  SIREQLNLYWPTLLFKDNKSFWNHEWMIHGECSDPPFNPFQYFQKTLKIRKTYDLLAILN 155
            ++ QLN  WP +L  +N+ FW+HEW  HG CS+  FN   YF+  + +R  YD++  L 
Sbjct: 61  HLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALR 120

Query: 156 AAGLGPTTAALRNRDDIEKAIGAAIGQKPVLKCNTNTRTRDRQLSEVVLCLDEDGVKLID 214
               GP     ++R  I+  + A  G+ P L+C T+ +T+   L +VV C  +DG  LID
Sbjct: 121 PHAAGP-NGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTLID 180

BLAST of Cp4.1LG11g10020 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 128.6 bits (322), Expect = 8.9e-29
Identity = 71/204 (34.80%), Postives = 106/204 (51.96%), Query Frame = 0

Query: 36  YEYFQMVQQWPQSICNSEH-----CIGKPKAMFTIHGLWPQNYSTSL--ICD-GSPFADE 95
           +++F  VQQWP S C+++        GKP A F IHGLWP N   +    CD  SP+   
Sbjct: 29  FDFFYFVQQWPGSYCDTKQSCCYPTTGKPAADFGIHGLWPNNNDGTYPSNCDPNSPYDQS 88

Query: 96  YIKSIREQLNLYWPTLLFK--DNKSFWNHEWMIHGECSDPPF-NPFQYFQKTLKIRKTYD 155
            I  +   +   WPTL        +FW+HEW  HG C++    N   YF+K L ++   D
Sbjct: 89  QISDLISSMQQNWPTLACPSGSGSTFWSHEWEKHGTCAESVLTNQHAYFKKALDLKNQID 148

Query: 156 LLAILNAAGLGPTTAALRNRDDIEKAIGAAIGQKPVLKCNTNTRTRDRQLSEVVLCLDED 215
           LL+IL  A + P   +  +  +I  AI +AIG  P ++CN + ++ + QL +V +C+D  
Sbjct: 149 LLSILQGADIHPDGESY-DLVNIRNAIKSAIGYTPWIQCNVD-QSGNSQLYQVYICVDGS 208

Query: 216 GVKLIDCAKFVSTNCPLTFSWPPF 229
           G  LI+C  F    C  +  +P F
Sbjct: 209 GSSLIECPIFPGGKCGTSIEFPTF 230

BLAST of Cp4.1LG11g10020 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 3.4e-28
Identity = 72/204 (35.29%), Postives = 104/204 (50.98%), Query Frame = 0

Query: 36  YEYFQMVQQWPQSICNSEH--CI---GKPKAMFTIHGLWPQNYSTSLI---CDGS-PFAD 95
           +++F  VQQWP S C+++   C    GKP A F IHGLWP NY        CD S PF  
Sbjct: 30  FDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWP-NYKDGTYPSNCDASKPFDS 89

Query: 96  EYIKSIREQLNLYWPTLLFK--DNKSFWNHEWMIHGECSDPPFNPFQYFQKTLKIRKTYD 155
             I  +   +   WPTL       ++FW HEW  HG CS+   +  +YFQ  L +++  +
Sbjct: 90  STISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQHEYFQTALNLKQKTN 149

Query: 156 LLAILNAAGLGPTTAALRNRDDIEKAIGAAIGQKPVLKCNTNTRTRDRQLSEVVLCLDED 215
           LL  L  AG+ P   +  + + I  +I  +IG  P ++CN +  + + QL +V LC+D  
Sbjct: 150 LLGALTKAGINPDGKSY-SLESIRDSIKESIGFTPWVECNRD-GSGNSQLYQVYLCVDRS 209

Query: 216 GVKLIDCAKFVSTNCPLTFSWPPF 229
           G  LI+C  F    C     +P F
Sbjct: 210 GSGLIECPVFPHGKCGAEIEFPSF 230

BLAST of Cp4.1LG11g10020 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 8.3e-27
Identity = 77/234 (32.91%), Postives = 110/234 (47.01%), Query Frame = 0

Query: 8   FFLFYLAFSS--VQAKFQEVERPKPKPKYQYEYFQMVQQWPQSICNSEHC-----IGKPK 67
           FF+F LA     VQ+  Q+           +++F  V QWP + C+S H       GKP 
Sbjct: 3   FFIFILALQQLYVQSFAQD-----------FDFFYFVLQWPGAYCDSRHSCCYPQTGKPA 62

Query: 68  AMFTIHGLWPQNYSTS---LICD-GSPFADEYIKSIREQLNLYWPTLL--FKDNKSFWNH 127
           A F IHGLWP NY T      C+  S F D  +  +   L   WPTL     D   FW H
Sbjct: 63  ADFGIHGLWP-NYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTH 122

Query: 128 EWMIHGECSDPPFNPFQYFQKTLKIRKTYDLLAILNAAGLGPTTAALRNRDDIEKAIGAA 187
           EW  HG C++   +   YF+  LK+++  +LL  L  AG+ P       + DIE  I   
Sbjct: 123 EWEKHGTCAESELDQHDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMK-DIENTIKQV 182

Query: 188 IGQKPVLKCNTNTRTRDRQLSEVVLCLDEDGVKLIDCAKFVSTNCPLTFSWPPF 229
           +G  P ++CN ++ + + QL ++ LC+D    K I+C       C     +P F
Sbjct: 183 VGFAPGIECNKDS-SHNSQLYQIYLCVDTSASKFINCPVMPHGRCDSRVQFPKF 222

BLAST of Cp4.1LG11g10020 vs. ExPASy Swiss-Prot
Match: O80323 (Ribonuclease S-3 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 6.2e-22
Identity = 70/190 (36.84%), Postives = 93/190 (48.95%), Query Frame = 0

Query: 36  YEYFQMVQQWPQSICNSEH--CIGKPKAMFTIHGLWPQNYSTSLICDGSPFADEYIKSIR 95
           Y+YFQ  QQ+  ++CNS    C   P  +FT+HGLWP N        G   +   IK+IR
Sbjct: 23  YDYFQFTQQYQLAVCNSNRTLCKDPPDKLFTVHGLWPSNMV------GPDPSKCPIKNIR 82

Query: 96  E-------QLNLYWPTLLFK-DNKSFWNHEWMIHGECSDPPF-NPFQYFQKTLK--IRKT 155
           +       QL + WP +  +  N  FW+ EWM HG C  P   N   YF+  +K  I K 
Sbjct: 83  KREKLLEHQLEIIWPNVFDRTKNNLFWDKEWMKHGSCGYPTIDNENHYFETVIKMYISKK 142

Query: 156 YDLLAILNAAGLGPTTAALRNRDDIEKAI-GAAIGQKPVLKCNTNTRTRDRQLSEVVLCL 212
            ++  IL+ A + P     R   DIE AI   A  +KP LKC     T   +L E+ LC 
Sbjct: 143 QNVSRILSKAKIEP-DGKKRALLDIENAIRNGADNKKPKLKCQKKGTT--TELVEITLCS 202

BLAST of Cp4.1LG11g10020 vs. NCBI nr
Match: XP_023546907.1 (ribonuclease MC-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 491 bits (1263), Expect = 2.22e-175
Identity = 232/232 (100.00%), Postives = 232/232 (100.00%), Query Frame = 0

Query: 1   MGNFVVTFFLFYLAFSSVQAKFQEVERPKPKPKYQYEYFQMVQQWPQSICNSEHCIGKPK 60
           MGNFVVTFFLFYLAFSSVQAKFQEVERPKPKPKYQYEYFQMVQQWPQSICNSEHCIGKPK
Sbjct: 1   MGNFVVTFFLFYLAFSSVQAKFQEVERPKPKPKYQYEYFQMVQQWPQSICNSEHCIGKPK 60

Query: 61  AMFTIHGLWPQNYSTSLICDGSPFADEYIKSIREQLNLYWPTLLFKDNKSFWNHEWMIHG 120
           AMFTIHGLWPQNYSTSLICDGSPFADEYIKSIREQLNLYWPTLLFKDNKSFWNHEWMIHG
Sbjct: 61  AMFTIHGLWPQNYSTSLICDGSPFADEYIKSIREQLNLYWPTLLFKDNKSFWNHEWMIHG 120

Query: 121 ECSDPPFNPFQYFQKTLKIRKTYDLLAILNAAGLGPTTAALRNRDDIEKAIGAAIGQKPV 180
           ECSDPPFNPFQYFQKTLKIRKTYDLLAILNAAGLGPTTAALRNRDDIEKAIGAAIGQKPV
Sbjct: 121 ECSDPPFNPFQYFQKTLKIRKTYDLLAILNAAGLGPTTAALRNRDDIEKAIGAAIGQKPV 180

Query: 181 LKCNTNTRTRDRQLSEVVLCLDEDGVKLIDCAKFVSTNCPLTFSWPPFVASE 232
           LKCNTNTRTRDRQLSEVVLCLDEDGVKLIDCAKFVSTNCPLTFSWPPFVASE
Sbjct: 181 LKCNTNTRTRDRQLSEVVLCLDEDGVKLIDCAKFVSTNCPLTFSWPPFVASE 232

BLAST of Cp4.1LG11g10020 vs. NCBI nr
Match: XP_022946024.1 (ribonuclease MC-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 478 bits (1231), Expect = 1.81e-170
Identity = 229/234 (97.86%), Postives = 230/234 (98.29%), Query Frame = 0

Query: 1   MGNFVVTFFLFYLAFSSVQAKFQEVERPKPKPK--YQYEYFQMVQQWPQSICNSEHCIGK 60
           MGNFVVTFFLFYLAFSSVQAKFQEVERPKPKPK  YQYEYFQMVQQWPQSICNSEHCIGK
Sbjct: 1   MGNFVVTFFLFYLAFSSVQAKFQEVERPKPKPKPKYQYEYFQMVQQWPQSICNSEHCIGK 60

Query: 61  PKAMFTIHGLWPQNYSTSLICDGSPFADEYIKSIREQLNLYWPTLLFKDNKSFWNHEWMI 120
           PKAMFTIHGLWPQNYSTSLIC+GS  ADEYIKSIREQLNLYWPTLLFKDNKSFWNHEWMI
Sbjct: 61  PKAMFTIHGLWPQNYSTSLICNGSQLADEYIKSIREQLNLYWPTLLFKDNKSFWNHEWMI 120

Query: 121 HGECSDPPFNPFQYFQKTLKIRKTYDLLAILNAAGLGPTTAALRNRDDIEKAIGAAIGQK 180
           HGECSDPPFNPFQYFQKTLKIRKTYDLLAILNAAGLGPTTAALRNRDDIEKAIGAAIGQK
Sbjct: 121 HGECSDPPFNPFQYFQKTLKIRKTYDLLAILNAAGLGPTTAALRNRDDIEKAIGAAIGQK 180

Query: 181 PVLKCNTNTRTRDRQLSEVVLCLDEDGVKLIDCAKFVSTNCPLTFSWPPFVASE 232
           PVLKCNTNTRTRDRQLSEVVLCLDEDGVKLIDCAKFVSTNCPLTFSWPPFVASE
Sbjct: 181 PVLKCNTNTRTRDRQLSEVVLCLDEDGVKLIDCAKFVSTNCPLTFSWPPFVASE 234

BLAST of Cp4.1LG11g10020 vs. NCBI nr
Match: XP_023546908.1 (ribonuclease MC-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 417 bits (1073), Expect = 7.79e-147
Identity = 205/232 (88.36%), Postives = 205/232 (88.36%), Query Frame = 0

Query: 1   MGNFVVTFFLFYLAFSSVQAKFQEVERPKPKPKYQYEYFQMVQQWPQSICNSEHCIGKPK 60
           MGNFVVTFFLFYLAFSSVQAKFQEVERPKPKPKYQYEYFQMVQQWPQSICNSEHCIGKPK
Sbjct: 1   MGNFVVTFFLFYLAFSSVQAKFQEVERPKPKPKYQYEYFQMVQQWPQSICNSEHCIGKPK 60

Query: 61  AMFTIHGLWPQNYSTSLICDGSPFADEYIKSIREQLNLYWPTLLFKDNKSFWNHEWMIHG 120
           AMFTIHGLWPQNYSTSLICDGSPFADEYIKSIREQLNLYWPTLLFKDNKSFWNHEWMIH 
Sbjct: 61  AMFTIHGLWPQNYSTSLICDGSPFADEYIKSIREQLNLYWPTLLFKDNKSFWNHEWMIH- 120

Query: 121 ECSDPPFNPFQYFQKTLKIRKTYDLLAILNAAGLGPTTAALRNRDDIEKAIGAAIGQKPV 180
                                     AILNAAGLGPTTAALRNRDDIEKAIGAAIGQKPV
Sbjct: 121 --------------------------AILNAAGLGPTTAALRNRDDIEKAIGAAIGQKPV 180

Query: 181 LKCNTNTRTRDRQLSEVVLCLDEDGVKLIDCAKFVSTNCPLTFSWPPFVASE 232
           LKCNTNTRTRDRQLSEVVLCLDEDGVKLIDCAKFVSTNCPLTFSWPPFVASE
Sbjct: 181 LKCNTNTRTRDRQLSEVVLCLDEDGVKLIDCAKFVSTNCPLTFSWPPFVASE 205

BLAST of Cp4.1LG11g10020 vs. NCBI nr
Match: XP_022946025.1 (ribonuclease MC-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 405 bits (1041), Expect = 6.33e-142
Identity = 202/234 (86.32%), Postives = 203/234 (86.75%), Query Frame = 0

Query: 1   MGNFVVTFFLFYLAFSSVQAKFQEVERPKPKPK--YQYEYFQMVQQWPQSICNSEHCIGK 60
           MGNFVVTFFLFYLAFSSVQAKFQEVERPKPKPK  YQYEYFQMVQQWPQSICNSEHCIGK
Sbjct: 1   MGNFVVTFFLFYLAFSSVQAKFQEVERPKPKPKPKYQYEYFQMVQQWPQSICNSEHCIGK 60

Query: 61  PKAMFTIHGLWPQNYSTSLICDGSPFADEYIKSIREQLNLYWPTLLFKDNKSFWNHEWMI 120
           PKAMFTIHGLWPQNYSTSLIC+GS  ADEYIKSIREQLNLYWPTLLFKDNKSFWNHEWMI
Sbjct: 61  PKAMFTIHGLWPQNYSTSLICNGSQLADEYIKSIREQLNLYWPTLLFKDNKSFWNHEWMI 120

Query: 121 HGECSDPPFNPFQYFQKTLKIRKTYDLLAILNAAGLGPTTAALRNRDDIEKAIGAAIGQK 180
           H                           AILNAAGLGPTTAALRNRDDIEKAIGAAIGQK
Sbjct: 121 H---------------------------AILNAAGLGPTTAALRNRDDIEKAIGAAIGQK 180

Query: 181 PVLKCNTNTRTRDRQLSEVVLCLDEDGVKLIDCAKFVSTNCPLTFSWPPFVASE 232
           PVLKCNTNTRTRDRQLSEVVLCLDEDGVKLIDCAKFVSTNCPLTFSWPPFVASE
Sbjct: 181 PVLKCNTNTRTRDRQLSEVVLCLDEDGVKLIDCAKFVSTNCPLTFSWPPFVASE 207

BLAST of Cp4.1LG11g10020 vs. NCBI nr
Match: XP_038906187.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 236 bits (601), Expect = 5.79e-74
Identity = 114/198 (57.58%), Postives = 134/198 (67.68%), Query Frame = 0

Query: 33  KYQYEYFQMVQQWPQSICNSE--HCIGKPKAMFTIHGLWPQNYSTS-LICDGSPFADEYI 92
           K QY+YFQMVQQWP + C+     C  +P +MFTIHGLWP NYST+ L+C G PF    I
Sbjct: 21  KAQYDYFQMVQQWPPATCSGVGVRCRAQPPSMFTIHGLWPSNYSTAHLVCTGLPFDPTQI 80

Query: 93  KSIREQLNLYWPTLLFKDNKSFWNHEWMIHGECSDPPFNPFQYFQKTLKIRKT--YDLLA 152
            +IR QLN YWP ++  +N+ FW HEW  HG CSDPPFN  QYFQ TL IR    YDLLA
Sbjct: 81  AAIRPQLNTYWPDVIRGNNQRFWQHEWDAHGTCSDPPFNQRQYFQTTLNIRMNHNYDLLA 140

Query: 153 ILNAAGLGPTTAALRNRDDIEKAIGAAIGQKPVLKCNTNTRTRDRQLSEVVLCLDEDGVK 212
           ILNAAGLGPT   +R    IE AI AA G+ P L+CNTN +T  RQL E++LC D++GV 
Sbjct: 141 ILNAAGLGPTATMVRQYQAIENAIQAATGKIPGLRCNTNAQTGSRQLFEIILCFDKNGVT 200

Query: 213 LIDCAKFVSTNCPLTFSW 225
           LIDC  F    CPL F W
Sbjct: 201 LIDCTPFAGITCPLQFVW 218

BLAST of Cp4.1LG11g10020 vs. ExPASy TrEMBL
Match: A0A6J1G2M1 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111450236 PE=3 SV=1)

HSP 1 Score: 478 bits (1231), Expect = 8.76e-171
Identity = 229/234 (97.86%), Postives = 230/234 (98.29%), Query Frame = 0

Query: 1   MGNFVVTFFLFYLAFSSVQAKFQEVERPKPKPK--YQYEYFQMVQQWPQSICNSEHCIGK 60
           MGNFVVTFFLFYLAFSSVQAKFQEVERPKPKPK  YQYEYFQMVQQWPQSICNSEHCIGK
Sbjct: 1   MGNFVVTFFLFYLAFSSVQAKFQEVERPKPKPKPKYQYEYFQMVQQWPQSICNSEHCIGK 60

Query: 61  PKAMFTIHGLWPQNYSTSLICDGSPFADEYIKSIREQLNLYWPTLLFKDNKSFWNHEWMI 120
           PKAMFTIHGLWPQNYSTSLIC+GS  ADEYIKSIREQLNLYWPTLLFKDNKSFWNHEWMI
Sbjct: 61  PKAMFTIHGLWPQNYSTSLICNGSQLADEYIKSIREQLNLYWPTLLFKDNKSFWNHEWMI 120

Query: 121 HGECSDPPFNPFQYFQKTLKIRKTYDLLAILNAAGLGPTTAALRNRDDIEKAIGAAIGQK 180
           HGECSDPPFNPFQYFQKTLKIRKTYDLLAILNAAGLGPTTAALRNRDDIEKAIGAAIGQK
Sbjct: 121 HGECSDPPFNPFQYFQKTLKIRKTYDLLAILNAAGLGPTTAALRNRDDIEKAIGAAIGQK 180

Query: 181 PVLKCNTNTRTRDRQLSEVVLCLDEDGVKLIDCAKFVSTNCPLTFSWPPFVASE 232
           PVLKCNTNTRTRDRQLSEVVLCLDEDGVKLIDCAKFVSTNCPLTFSWPPFVASE
Sbjct: 181 PVLKCNTNTRTRDRQLSEVVLCLDEDGVKLIDCAKFVSTNCPLTFSWPPFVASE 234

BLAST of Cp4.1LG11g10020 vs. ExPASy TrEMBL
Match: A0A6J1G2M7 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111450236 PE=3 SV=1)

HSP 1 Score: 405 bits (1041), Expect = 3.06e-142
Identity = 202/234 (86.32%), Postives = 203/234 (86.75%), Query Frame = 0

Query: 1   MGNFVVTFFLFYLAFSSVQAKFQEVERPKPKPK--YQYEYFQMVQQWPQSICNSEHCIGK 60
           MGNFVVTFFLFYLAFSSVQAKFQEVERPKPKPK  YQYEYFQMVQQWPQSICNSEHCIGK
Sbjct: 1   MGNFVVTFFLFYLAFSSVQAKFQEVERPKPKPKPKYQYEYFQMVQQWPQSICNSEHCIGK 60

Query: 61  PKAMFTIHGLWPQNYSTSLICDGSPFADEYIKSIREQLNLYWPTLLFKDNKSFWNHEWMI 120
           PKAMFTIHGLWPQNYSTSLIC+GS  ADEYIKSIREQLNLYWPTLLFKDNKSFWNHEWMI
Sbjct: 61  PKAMFTIHGLWPQNYSTSLICNGSQLADEYIKSIREQLNLYWPTLLFKDNKSFWNHEWMI 120

Query: 121 HGECSDPPFNPFQYFQKTLKIRKTYDLLAILNAAGLGPTTAALRNRDDIEKAIGAAIGQK 180
           H                           AILNAAGLGPTTAALRNRDDIEKAIGAAIGQK
Sbjct: 121 H---------------------------AILNAAGLGPTTAALRNRDDIEKAIGAAIGQK 180

Query: 181 PVLKCNTNTRTRDRQLSEVVLCLDEDGVKLIDCAKFVSTNCPLTFSWPPFVASE 232
           PVLKCNTNTRTRDRQLSEVVLCLDEDGVKLIDCAKFVSTNCPLTFSWPPFVASE
Sbjct: 181 PVLKCNTNTRTRDRQLSEVVLCLDEDGVKLIDCAKFVSTNCPLTFSWPPFVASE 207

BLAST of Cp4.1LG11g10020 vs. ExPASy TrEMBL
Match: A0A6J1EXD5 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1)

HSP 1 Score: 231 bits (588), Expect = 2.15e-72
Identity = 114/225 (50.67%), Postives = 139/225 (61.78%), Query Frame = 0

Query: 4   FVVTFFLFYLAFSSVQAKFQEVERPKPKPKYQYEYFQMVQQWPQSICNSEHCIGKPKAMF 63
           F+V+ F F L  S V+A              Q++YFQMVQQWP + C+   C   P AMF
Sbjct: 6   FIVSIFKFVLLTSCVKA--------------QFDYFQMVQQWPPATCSGVRCYANPPAMF 65

Query: 64  TIHGLWPQNYST-SLICDGSPFADEYIKSIREQLNLYWPTLLFKDNKSFWNHEWMIHGEC 123
           TIHGLWP NYS   L+C G PF    I ++  QLN YWP ++  +N+ FW HEW  HG C
Sbjct: 66  TIHGLWPSNYSNVPLVCAGQPFNPAQIATLVPQLNTYWPDVISGNNQRFWKHEWDSHGTC 125

Query: 124 SDPPFNPFQYFQKTLKIRKT--YDLLAILNAAGLGPTTAALRNRDDIEKAIGAAIGQKPV 183
           SDPPFN  QYFQ TL IR    YDLLAILN AGLGPT    R    IE AI AA G+KP 
Sbjct: 126 SDPPFNQLQYFQTTLNIRTNHNYDLLAILNTAGLGPTATNTRQYGAIEGAIQAATGKKPG 185

Query: 184 LKCNTNTRTRDRQLSEVVLCLDEDGVKLIDCAKFVSTNCPLTFSW 225
           L+CN N +++ +QL E++LC D++GV LIDC +F    CP  F W
Sbjct: 186 LRCNKNAQSKKKQLFEIILCFDKNGVTLIDCTQFAGITCPSQFVW 216

BLAST of Cp4.1LG11g10020 vs. ExPASy TrEMBL
Match: A0A6J1IAR7 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111472844 PE=3 SV=1)

HSP 1 Score: 223 bits (569), Expect = 1.83e-69
Identity = 104/196 (53.06%), Postives = 127/196 (64.80%), Query Frame = 0

Query: 33  KYQYEYFQMVQQWPQSICNSEHCIGKPKAMFTIHGLWPQNYST-SLICDGSPFADEYIKS 92
           K Q++YFQMVQQWP + C+   C   P AMFTIHGLWP  YS    +C G PF    I +
Sbjct: 22  KAQFDYFQMVQQWPPATCSGVRCYANPPAMFTIHGLWPSTYSNVPPVCAGQPFDRAQIAT 81

Query: 93  IREQLNLYWPTLLFKDNKSFWNHEWMIHGECSDPPFNPFQYFQKTLKIRKT--YDLLAIL 152
           +  QLN YWP ++  +N+ FW HEW  HG CSDPPFN  QYFQ TL IR    YDLLAIL
Sbjct: 82  LEPQLNTYWPDVINGNNQRFWKHEWDNHGTCSDPPFNQLQYFQTTLNIRTNHNYDLLAIL 141

Query: 153 NAAGLGPTTAALRNRDDIEKAIGAAIGQKPVLKCNTNTRTRDRQLSEVVLCLDEDGVKLI 212
           N AGLGPT    R    IE AI AA G+KP L+CN N +++ +QL E++LC D++GV LI
Sbjct: 142 NTAGLGPTATTTRQYQAIEGAIQAATGKKPGLRCNKNAQSKRKQLFEIILCFDKNGVTLI 201

Query: 213 DCAKFVSTNCPLTFSW 225
           +C +F    CP  F W
Sbjct: 202 NCTQFAGITCPSQFVW 217

BLAST of Cp4.1LG11g10020 vs. ExPASy TrEMBL
Match: A0A6J1F837 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441728 PE=3 SV=1)

HSP 1 Score: 221 bits (562), Expect = 5.95e-69
Identity = 106/198 (53.54%), Postives = 134/198 (67.68%), Query Frame = 0

Query: 35  QYEYFQMVQQWPQSICNSE--HCIGKPKAMFTIHGLWPQNYSTS-LICDGSPFADEYIKS 94
           Q++YFQMVQQWP + C+S    C  +P  MFTIHGLWP NYS + L+C   PF    I +
Sbjct: 25  QFDYFQMVQQWPPAKCSSVKVRCHVQPPTMFTIHGLWPSNYSNARLVCAPQPFNPTQIAA 84

Query: 95  IREQLNLYWPTLLFKDNKSFWNHEWMIHGECSDPPFNPFQYFQKTLKIRK--TYDLLAIL 154
           +R QLN YWP ++   N+ FW HEW  HG CS+PPFN  QYFQ TL IR   +YDLL IL
Sbjct: 85  LRPQLNTYWPDVIQGKNQQFWQHEWDRHGICSNPPFNQLQYFQITLNIRTNHSYDLLTIL 144

Query: 155 NAAGLGPTTAALRNRDDIEKAIGAAIGQKPVLKCNTNTRTRDR-QLSEVVLCLDEDGVKL 214
           NAAGLGP+   +R   DIE AI AA G+KP L+CN N +++++ QL E++LC D+DG+ L
Sbjct: 145 NAAGLGPSDTNIREYKDIEGAIQAATGKKPGLRCNINAQSKEKKQLLEIILCFDKDGITL 204

Query: 215 IDCAKFVSTNCPLTFSWP 226
           IDC +F    CP  F WP
Sbjct: 205 IDCTEFAGITCPPQFVWP 222

BLAST of Cp4.1LG11g10020 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 129.4 bits (324), Expect = 3.7e-30
Identity = 72/209 (34.45%), Postives = 105/209 (50.24%), Query Frame = 0

Query: 31  KPKYQYEYFQMVQQWPQSICNSEHCI-----GKPKAMFTIHGLWPQNY---STSLICD-G 90
           +P+  +++F  V QWP + C++         GKP A F IHGLWP NY   S    CD  
Sbjct: 23  RPQEDFDFFYFVLQWPGAYCDTSRACCYPTSGKPAADFGIHGLWP-NYNGGSWPSNCDPD 82

Query: 91  SPFADEYIKSIREQLNLYWPTLLFKDNK--SFWNHEWMIHGECSDPPFNPFQYFQKTLKI 150
           S F    I  +   L   WPTL    N+  +FW HEW  HG CS+   +  +YF+  LK+
Sbjct: 83  SQFDRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSESVMDQHEYFENALKL 142

Query: 151 RKTYDLLAILNAAGLGPTTAALRNRDDIEKAIGAAIGQKPVLKCNTNTRTRDRQLSEVVL 210
           ++  +LL IL  +G+ P      N D I  AI   IG  P ++CN +   R+ QL ++ +
Sbjct: 143 KQKANLLQILKNSGINPDD-GFYNLDKITNAIKDGIGFTPGIECNKDPE-RNAQLHQIYI 202

Query: 211 CLDEDGVKLIDCAKFVSTNCPLTFSWPPF 229
           C+D  G + I+C      +CP    +  F
Sbjct: 203 CVDTSGTEFIECPVLPRGSCPSQIQFSKF 228

BLAST of Cp4.1LG11g10020 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 126.7 bits (317), Expect = 2.4e-29
Identity = 72/204 (35.29%), Postives = 104/204 (50.98%), Query Frame = 0

Query: 36  YEYFQMVQQWPQSICNSEH--CI---GKPKAMFTIHGLWPQNYSTSLI---CDGS-PFAD 95
           +++F  VQQWP S C+++   C    GKP A F IHGLWP NY        CD S PF  
Sbjct: 30  FDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWP-NYKDGTYPSNCDASKPFDS 89

Query: 96  EYIKSIREQLNLYWPTLLFK--DNKSFWNHEWMIHGECSDPPFNPFQYFQKTLKIRKTYD 155
             I  +   +   WPTL       ++FW HEW  HG CS+   +  +YFQ  L +++  +
Sbjct: 90  STISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQHEYFQTALNLKQKTN 149

Query: 156 LLAILNAAGLGPTTAALRNRDDIEKAIGAAIGQKPVLKCNTNTRTRDRQLSEVVLCLDED 215
           LL  L  AG+ P   +  + + I  +I  +IG  P ++CN +  + + QL +V LC+D  
Sbjct: 150 LLGALTKAGINPDGKSY-SLESIRDSIKESIGFTPWVECNRD-GSGNSQLYQVYLCVDRS 209

Query: 216 GVKLIDCAKFVSTNCPLTFSWPPF 229
           G  LI+C  F    C     +P F
Sbjct: 210 GSGLIECPVFPHGKCGAEIEFPSF 230

BLAST of Cp4.1LG11g10020 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 122.1 bits (305), Expect = 5.9e-28
Identity = 77/234 (32.91%), Postives = 110/234 (47.01%), Query Frame = 0

Query: 8   FFLFYLAFSS--VQAKFQEVERPKPKPKYQYEYFQMVQQWPQSICNSEHC-----IGKPK 67
           FF+F LA     VQ+  Q+           +++F  V QWP + C+S H       GKP 
Sbjct: 3   FFIFILALQQLYVQSFAQD-----------FDFFYFVLQWPGAYCDSRHSCCYPQTGKPA 62

Query: 68  AMFTIHGLWPQNYSTS---LICD-GSPFADEYIKSIREQLNLYWPTLL--FKDNKSFWNH 127
           A F IHGLWP NY T      C+  S F D  +  +   L   WPTL     D   FW H
Sbjct: 63  ADFGIHGLWP-NYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTH 122

Query: 128 EWMIHGECSDPPFNPFQYFQKTLKIRKTYDLLAILNAAGLGPTTAALRNRDDIEKAIGAA 187
           EW  HG C++   +   YF+  LK+++  +LL  L  AG+ P       + DIE  I   
Sbjct: 123 EWEKHGTCAESELDQHDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMK-DIENTIKQV 182

Query: 188 IGQKPVLKCNTNTRTRDRQLSEVVLCLDEDGVKLIDCAKFVSTNCPLTFSWPPF 229
           +G  P ++CN ++ + + QL ++ LC+D    K I+C       C     +P F
Sbjct: 183 VGFAPGIECNKDS-SHNSQLYQIYLCVDTSASKFINCPVMPHGRCDSRVQFPKF 222

BLAST of Cp4.1LG11g10020 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 99.8 bits (247), Expect = 3.1e-21
Identity = 60/203 (29.56%), Postives = 93/203 (45.81%), Query Frame = 0

Query: 36  YEYFQMVQQWPQSICNSEH-CI----GKPKAMFTIHGLWPQ--NYSTSLICDGSPFAD-E 95
           + +F  V  WP +IC+S+  C     G   + F IHGLWPQ  N +    CD +   D  
Sbjct: 26  FNFFYWVNYWPGAICDSQKGCCPPTKGNTASDFIIHGLWPQFNNGTWPAFCDQTNLFDIS 85

Query: 96  YIKSIREQLNLYWPT----LLFKDNKSFWNHEWMIHGECSDPPFNPFQYFQKTLKIRKTY 155
            I  +  Q+   W          +  + W HEW  HG C    F+   YF+  LK +   
Sbjct: 86  KISDLVCQMEKKWTEWGVWACPSNETNLWEHEWNKHGTCVQSIFDQHSYFRTNLKFKHKV 145

Query: 156 DLLAILNAAGLGPTTAALRNRDDIEKAIGAAIGQKPVLKCNTNTRTRDRQLSEVVLCLDE 215
            LL IL   G+ P      + D+I+ AI  AIG  P ++CN + +  ++QL ++ +CLD 
Sbjct: 146 HLLNILIQKGIKPND-GFYSLDEIKNAIKCAIGFAPGIECNEDVK-GNKQLFQIYICLDN 205

Query: 216 DGVKLIDCAKFVSTNCPLTFSWP 227
              + ++C      +C     +P
Sbjct: 206 YAKEFVECPYVPDKSCASKIKFP 226

BLAST of Cp4.1LG11g10020 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 88.6 bits (218), Expect = 7.3e-18
Identity = 62/217 (28.57%), Postives = 92/217 (42.40%), Query Frame = 0

Query: 35  QYEYFQMVQQWPQSIC-NSEHCIGK--------PKAMFTIHGLWP--QNYSTSLICDGSP 94
           +++YF +  QWP + C  + HC  K            FTIHGLWP   + S    C  S 
Sbjct: 31  EFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSD 90

Query: 95  FADEYIKSIREQLNLYWPTLLFKD-------NKSFWNHEWMIHGECSDPPF-NPFQYFQK 154
           F ++ I ++ + L  YWP+L             SFW HEW  HG CS P F + + YF  
Sbjct: 91  FKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNYFLT 150

Query: 155 TLKIRKTYDLLAILNAAGLGPTTAALRNRDDIEKAIGAAIGQKPVLKCNTNTRTRDRQLS 214
           TL +   +++  +L  AG   + +       I  AI  A    P + C  +       + 
Sbjct: 151 TLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVCKRDA------ID 210

Query: 215 EVVLCLDEDGVKLIDCAKFVS----TNCPLTFSWPPF 229
           E+ +C  +D  K  DC          +CP   S P +
Sbjct: 211 EIRICFYKD-FKPRDCVGSQDLTSRKSCPKYVSLPEY 240

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P235401.1e-3439.89Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1[more]
P800228.9e-2934.80Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2[more]
P428133.4e-2835.29Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1[more]
P428158.3e-2732.91Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1[more]
O803236.2e-2236.84Ribonuclease S-3 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_023546907.12.22e-175100.00ribonuclease MC-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022946024.11.81e-17097.86ribonuclease MC-like isoform X1 [Cucurbita moschata][more]
XP_023546908.17.79e-14788.36ribonuclease MC-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022946025.16.33e-14286.32ribonuclease MC-like isoform X2 [Cucurbita moschata][more]
XP_038906187.15.79e-7457.58ribonuclease MC-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1G2M18.76e-17197.86Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111450236 PE=3 SV=1[more]
A0A6J1G2M73.06e-14286.32Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111450236 PE=3 SV=1[more]
A0A6J1EXD52.15e-7250.67Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1[more]
A0A6J1IAR71.83e-6953.06Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111472844 PE=3 SV=1[more]
A0A6J1F8375.95e-6953.54Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441728 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G14220.13.7e-3034.45Ribonuclease T2 family protein [more]
AT2G02990.12.4e-2935.29ribonuclease 1 [more]
AT1G26820.15.9e-2832.91ribonuclease 3 [more]
AT1G14210.13.1e-2129.56Ribonuclease T2 family protein [more]
AT2G39780.17.3e-1828.57ribonuclease 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001568Ribonuclease T2-likePFAMPF00445Ribonuclease_T2coord: 37..211
e-value: 5.9E-43
score: 147.2
IPR001568Ribonuclease T2-likePANTHERPTHR11240RIBONUCLEASE T2coord: 26..220
IPR036430Ribonuclease T2-like superfamilyGENE3D3.90.730.10coord: 31..231
e-value: 9.7E-53
score: 181.2
IPR036430Ribonuclease T2-like superfamilySUPERFAMILY55895Ribonuclease Rh-likecoord: 36..227
NoneNo IPR availablePANTHERPTHR11240:SF49RIBONUCLEASE 3coord: 26..220
IPR018188Ribonuclease T2, His active site 1PROSITEPS00530RNASE_T2_1coord: 63..70
IPR033697Ribonuclease T2, eukaryoticCDDcd01061RNase_T2_eukcoord: 36..222
e-value: 2.07358E-60
score: 186.384

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG11g10020.1Cp4.1LG11g10020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function GO:0033897 ribonuclease T2 activity
molecular_function GO:0003723 RNA binding