Cp4.1LG11g09390 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG11g09390
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Kunitz-type trypsin inhibitor
Location	Cp4.1LG11: 8022995 .. 8023576 (+)
RNA-Seq Expression	Cp4.1LG11g09390
Synteny	Cp4.1LG11g09390

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGCATAATTCACTTGCCATCTTTAGCTACCTATTAGTCTTCATGGCCATAACTTCCACAGCTCAACTTCCTCCAGTGCTCGACACCGACGGTCAACCTCTACAACGTGGTGTTGAGTACTACATCAAGCCTGCAATCACCGACGTTGCAGGTAATCTCACCCTAAAAAGTCGTAGCAATGCTCCTTGCCCGCTCTACGTCGGTCAAGAACCAGTTGCTTCAACAAATATCGGTCTCCCAGTCACCTTCACTCCCATCGTGGCGGGCGAAGATATAATTGAAGAAAGTATGGGTTTCAATGTTGCGTTTCAAGCATCGTCGACGTGCATAACATCGACACAATGGAGAGTGGATGAGATGGAGTCTGCAACAGGGAGGAGGTTCGTGGGGGTCGGGAACGATAACAGTCCGACTGGGATCTTTAGGATCGACAGGAACAATGGTGTTTACAATATAGTATGGTGCCCTGAGATGGTAGGGAGGCCAAGGTGTGGAAGTGCTGGGATTTTGGTGGAGGATGGAGTGAGGCTGCTGGCTTTGGATGGCAATGCGTTTCCTTTTGAGTTTGTCAAAGCTTGA

mRNA sequence

ATGTTGCATAATTCACTTGCCATCTTTAGCTACCTATTAGTCTTCATGGCCATAACTTCCACAGCTCAACTTCCTCCAGTGCTCGACACCGACGGTCAACCTCTACAACGTGGTGTTGAGTACTACATCAAGCCTGCAATCACCGACGTTGCAGGTAATCTCACCCTAAAAAGTCGTAGCAATGCTCCTTGCCCGCTCTACGTCGGTCAAGAACCAGTTGCTTCAACAAATATCGGTCTCCCAGTCACCTTCACTCCCATCGTGGCGGGCGAAGATATAATTGAAGAAAGTATGGGTTTCAATGTTGCGTTTCAAGCATCGTCGACGTGCATAACATCGACACAATGGAGAGTGGATGAGATGGAGTCTGCAACAGGGAGGAGGTTCGTGGGGGTCGGGAACGATAACAGTCCGACTGGGATCTTTAGGATCGACAGGAACAATGGTGTTTACAATATAGTATGGTGCCCTGAGATGGTAGGGAGGCCAAGGTGTGGAAGTGCTGGGATTTTGGTGGAGGATGGAGTGAGGCTGCTGGCTTTGGATGGCAATGCGTTTCCTTTTGAGTTTGTCAAAGCTTGA

Coding sequence (CDS)

ATGTTGCATAATTCACTTGCCATCTTTAGCTACCTATTAGTCTTCATGGCCATAACTTCCACAGCTCAACTTCCTCCAGTGCTCGACACCGACGGTCAACCTCTACAACGTGGTGTTGAGTACTACATCAAGCCTGCAATCACCGACGTTGCAGGTAATCTCACCCTAAAAAGTCGTAGCAATGCTCCTTGCCCGCTCTACGTCGGTCAAGAACCAGTTGCTTCAACAAATATCGGTCTCCCAGTCACCTTCACTCCCATCGTGGCGGGCGAAGATATAATTGAAGAAAGTATGGGTTTCAATGTTGCGTTTCAAGCATCGTCGACGTGCATAACATCGACACAATGGAGAGTGGATGAGATGGAGTCTGCAACAGGGAGGAGGTTCGTGGGGGTCGGGAACGATAACAGTCCGACTGGGATCTTTAGGATCGACAGGAACAATGGTGTTTACAATATAGTATGGTGCCCTGAGATGGTAGGGAGGCCAAGGTGTGGAAGTGCTGGGATTTTGGTGGAGGATGGAGTGAGGCTGCTGGCTTTGGATGGCAATGCGTTTCCTTTTGAGTTTGTCAAAGCTTGA

Protein sequence

MLHNSLAIFSYLLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEMESATGRRFVGVGNDNSPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA

Homology

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BLAST of Cp4.1LG11g09390 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 1.1e-29
Identity = 78/199 (39.20%), Postives = 115/199 (57.79%), Query Frame = 0


Query: 5   SLAIFSYLLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRSNAPC 64
           S  IF    V++ + +T+    V+DT G+P++   +Y+I+PAIT   G+LTL +R++  C
Sbjct: 5   SFTIFILANVWLLVVTTSIAQFVIDTSGEPVENDEDYFIRPAITGNGGSLTLVTRNS--C 64

Query: 65  PLYVGQEPVASTNIGLPVTFTPIVAG--EDIIEESMGFNVAFQASSTCITSTQWRVDEME 124
           P  VG +P A    G  V  +P V+   ED +       V FQA ++C  ST+WR+ E +
Sbjct: 65  PFNVGLDPDAPQ--GFAVLLSPFVSNREEDEVRLGRDLRVIFQAGTSCGQSTEWRLGERD 124

Query: 125 SATGRRFVGVGNDNSPTG----IFRIDR--NNGVYNIVWCPEMV---GRPRCGSAGILVE 184
           + TGRRF+  G D+S  G     FRI +  + G++NI WCP  V    +  CG+ GI+ E
Sbjct: 125 ATTGRRFIITGRDDSTVGSYGNFFRIVQTPSRGIFNIQWCPTEVCPSCKFECGTVGIVRE 184

Query: 185 DGVRLLALDGNAFPFEFVK 193
           +G  LLALDG+A P  F K
Sbjct: 185 NGKILLALDGSALPVAFQK 199

[Close]

BLAST of Cp4.1LG11g09390 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 1.5e-29
Identity = 81/204 (39.71%), Postives = 114/204 (55.88%), Query Frame = 0


Query: 1   MLHNSLAIF--SYLLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKS 60
           M   SL IF  +++ + MA TS AQ   V+DT G+P++   EY+I+PAIT   G  TL +
Sbjct: 1   MSTRSLTIFILAHVWLLMATTSIAQF--VIDTSGEPVEDDEEYFIRPAITGNGGGSTLVT 60

Query: 61  RSNAPCPLYVGQEPVASTNIGLPVTFTPIVA--GEDIIEESMGFNVAFQASSTCITSTQW 120
             N PCPL+VG +    T +G+ V FTP      +D +  +    V F  S++C  ST W
Sbjct: 61  -GNGPCPLHVGLDNTEGT-LGVAVKFTPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDW 120

Query: 121 RVDEMESATGRRFVGVGNDN---SPTGIFRI--DRNNGVYNIVWCPEMV---GRPRCGSA 180
           R+ E ++ +GRR +  G DN   S    FRI   +  G YNI WCP       + +CG+ 
Sbjct: 121 RLGEKDATSGRRLIVTGRDNGAGSQGNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTV 180

Query: 181 GILVEDGVRLLALDGNAFPFEFVK 193
           G++ E+G  LLALDG+A P  F K
Sbjct: 181 GVIRENGKNLLALDGDALPVVFQK 200

[Close]

BLAST of Cp4.1LG11g09390 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 3.3e-29
Identity = 78/199 (39.20%), Postives = 112/199 (56.28%), Query Frame = 0


Query: 5   SLAIFSYLLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRSNAPC 64
           +L I +++ +F+  T+ AQ   VLDT G+P++   EY+I+P IT+  G  T+ SR N  C
Sbjct: 9   TLIILAHVCLFITTTTIAQF--VLDTVGEPVEGDEEYFIRPVITNKGGRSTMVSR-NESC 68

Query: 65  PLYVGQEPVASTNIGLPVTFTPIVAGEDI--IEESMGFNVAFQASSTCITSTQWRVDEME 124
           PL+VG E +     GL V FTP     D   +  +    + FQASS+C+ ST+WR+ E +
Sbjct: 69  PLHVGLE-LTGLGRGLVVKFTPFAPHHDFDDVRVNRDLRITFQASSSCVQSTEWRLGEKD 128

Query: 125 SATGRRFVGVGNDNSPTG----IFRIDRN--NGVYNIVWCPEMV---GRPRCGSAGILVE 184
           + +GRR +  G D++  G     FRI      G+YNI WCP  V    +  CG+  +L E
Sbjct: 129 TKSGRRLIITGTDSATNGSYGNFFRIVETPLEGMYNIQWCPTEVCPSCKFECGTVDMLNE 188

Query: 185 DGVRLLALDGNAFPFEFVK 193
           +G  LLALDG   P  F K
Sbjct: 189 NGKILLALDGGPLPLVFQK 203

[Close]

BLAST of Cp4.1LG11g09390 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 96.7 bits (239), Expect = 3.1e-19
Identity = 63/183 (34.43%), Postives = 90/183 (49.18%), Query Frame = 0


Query: 26  PVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKS---RSNAPCPLYVGQEPVASTNIGLPV 85
           PVLD DG+ L+ G  YYI P + D  G LT+ +        CP  V Q      +   P+
Sbjct: 35  PVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTPNGTFVCPPRVVQTR-KEVDHDRPL 94

Query: 86  TFTPIVAGEDIIEESMGFNVAFQASSTC--ITSTQWRVDEMESATGRRFVGVGNDNSPTG 145
            F P    ED++  S   N+ F A   C   +ST WR+D+ + +TG+ FV +G      G
Sbjct: 95  AFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWRLDKYDESTGQYFVTIGGVKGNPG 154

Query: 146 ------IFRIDR--NNGVYNIVWCPEMVG--RPRCGSAGILVED-GVRLLALDGNAFPFE 193
                  F+I+    +G Y +V+CP + G  + +CG  GI ++  G R LAL    F FE
Sbjct: 155 PETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKCGDVGIYIDQKGRRRLALSDKPFAFE 214

[Close]

BLAST of Cp4.1LG11g09390 vs. ExPASy Swiss-Prot
Match: P07596 (Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2)

HSP 1 Score: 85.5 bits (210), Expect = 7.2e-16
Identity = 55/153 (35.95%), Postives = 78/153 (50.98%), Query Frame = 0


Query: 22  AQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPVASTNIGLP 81
           A  PPV DTDG  L+    YY+  A     G LT+       CPL+V Q+P    + G P
Sbjct: 23  ADPPPVHDTDGHELRADANYYVLSANRAHGGGLTMAPGHGRHCPLFVSQDPNGQHD-GFP 82

Query: 82  VTFTP--IVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEMESATGRRFVGVG--NDNS 141
           V  TP  +   + II  S    ++F+A +TC+ ST+W +D  E A GRR V  G   D S
Sbjct: 83  VRITPYGVAPSDKIIRLSTDVRISFRAYTTCLQSTEWHIDS-ELAAGRRHVITGPVKDPS 142

Query: 142 PTG---IFRIDRNNGV----YNIV----WCPEM 160
           P+G    FRI++ +G     Y ++    WC ++
Sbjct: 143 PSGRENAFRIEKYSGAEVHEYKLMSCGDWCQDL 173

[Close]

BLAST of Cp4.1LG11g09390 vs. NCBI nr
Match: XP_023545477.1 (miraculin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 392 bits (1007), Expect = 1.21e-137
Identity = 193/193 (100.00%), Postives = 193/193 (100.00%), Query Frame = 0


Query: 1   MLHNSLAIFSYLLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRS 60
           MLHNSLAIFSYLLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRS
Sbjct: 1   MLHNSLAIFSYLLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRS 60

Query: 61  NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE 120
           NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE
Sbjct: 61  NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE 120

Query: 121 MESATGRRFVGVGNDNSPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180
           MESATGRRFVGVGNDNSPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA
Sbjct: 121 MESATGRRFVGVGNDNSPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 181 LDGNAFPFEFVKA 193
           LDGNAFPFEFVKA
Sbjct: 181 LDGNAFPFEFVKA 193

[Close]

BLAST of Cp4.1LG11g09390 vs. NCBI nr
Match: XP_022946294.1 (miraculin-like [Cucurbita moschata] >XP_022946295.1 miraculin-like [Cucurbita moschata])

HSP 1 Score: 385 bits (990), Expect = 4.72e-135
Identity = 188/193 (97.41%), Postives = 191/193 (98.96%), Query Frame = 0


Query: 1   MLHNSLAIFSYLLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRS 60
           MLHNSLAIFS+LLVFMAITSTAQLPPVLDTDGQPL+RGVEYYIKPAITDVAGNLTLKSRS
Sbjct: 1   MLHNSLAIFSFLLVFMAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRS 60

Query: 61  NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE 120
           NAPCPLYVGQEP+ASTNIGLPVTFTPIVAGEDIIEESMGFNV FQASSTCITSTQWRVDE
Sbjct: 61  NAPCPLYVGQEPIASTNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDE 120

Query: 121 MESATGRRFVGVGNDNSPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180
           MESATGRRFVGVGNDN PTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA
Sbjct: 121 MESATGRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 181 LDGNAFPFEFVKA 193
           LDGNAFPFEFVKA
Sbjct: 181 LDGNAFPFEFVKA 193

[Close]

BLAST of Cp4.1LG11g09390 vs. NCBI nr
Match: XP_022999136.1 (miraculin-like [Cucurbita maxima])

HSP 1 Score: 385 bits (988), Expect = 9.52e-135
Identity = 188/193 (97.41%), Postives = 192/193 (99.48%), Query Frame = 0


Query: 1   MLHNSLAIFSYLLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRS 60
           MLHNSLAIFSYLLVF+AITSTAQLPPVLDTDGQPL+RGVEYYIKPAIT+VAGNLTLK+RS
Sbjct: 1   MLHNSLAIFSYLLVFLAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITNVAGNLTLKNRS 60

Query: 61  NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE 120
           NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE
Sbjct: 61  NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE 120

Query: 121 MESATGRRFVGVGNDNSPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180
           MESATGRRFVGVGNDN PTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA
Sbjct: 121 MESATGRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 181 LDGNAFPFEFVKA 193
           LDGNAFPFEFVKA
Sbjct: 181 LDGNAFPFEFVKA 193

[Close]

BLAST of Cp4.1LG11g09390 vs. NCBI nr
Match: KAG7030262.1 (hypothetical protein SDJN02_08609, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 361 bits (926), Expect = 1.59e-125
Identity = 174/178 (97.75%), Postives = 176/178 (98.88%), Query Frame = 0


Query: 16  MAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPVAS 75
           MAITSTAQLPPVLDTDGQPL+RGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEP+AS
Sbjct: 1   MAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPIAS 60

Query: 76  TNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEMESATGRRFVGVGND 135
           TNIGLPVTFTPIVAGEDIIEESMGFNV FQASSTCITSTQWRVDEMESATGRRFVGVGND
Sbjct: 61  TNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRFVGVGND 120

Query: 136 NSPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA 193
           N PTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA
Sbjct: 121 NGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA 178

[Close]

BLAST of Cp4.1LG11g09390 vs. NCBI nr
Match: KAG6599263.1 (hypothetical protein SDJN03_09041, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 359 bits (921), Expect = 9.21e-125
Identity = 172/178 (96.63%), Postives = 176/178 (98.88%), Query Frame = 0


Query: 16  MAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPVAS 75
           MAITSTAQLPPVLDTDGQPL+RGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEP+AS
Sbjct: 1   MAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPIAS 60

Query: 76  TNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEMESATGRRFVGVGND 135
           TNIGLPVTFTPIVAGEDIIEES+GFNV FQASSTC+TSTQWRVDEMESATGRRFVGVGND
Sbjct: 61  TNIGLPVTFTPIVAGEDIIEESIGFNVVFQASSTCVTSTQWRVDEMESATGRRFVGVGND 120

Query: 136 NSPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA 193
           N PTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA
Sbjct: 121 NGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA 178

[Close]

BLAST of Cp4.1LG11g09390 vs. ExPASy TrEMBL
Match: A0A6J1G3G3 (miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1)

HSP 1 Score: 385 bits (990), Expect = 2.28e-135
Identity = 188/193 (97.41%), Postives = 191/193 (98.96%), Query Frame = 0


Query: 1   MLHNSLAIFSYLLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRS 60
           MLHNSLAIFS+LLVFMAITSTAQLPPVLDTDGQPL+RGVEYYIKPAITDVAGNLTLKSRS
Sbjct: 1   MLHNSLAIFSFLLVFMAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRS 60

Query: 61  NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE 120
           NAPCPLYVGQEP+ASTNIGLPVTFTPIVAGEDIIEESMGFNV FQASSTCITSTQWRVDE
Sbjct: 61  NAPCPLYVGQEPIASTNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDE 120

Query: 121 MESATGRRFVGVGNDNSPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180
           MESATGRRFVGVGNDN PTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA
Sbjct: 121 MESATGRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 181 LDGNAFPFEFVKA 193
           LDGNAFPFEFVKA
Sbjct: 181 LDGNAFPFEFVKA 193

[Close]

BLAST of Cp4.1LG11g09390 vs. ExPASy TrEMBL
Match: A0A6J1KC85 (miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1)

HSP 1 Score: 385 bits (988), Expect = 4.61e-135
Identity = 188/193 (97.41%), Postives = 192/193 (99.48%), Query Frame = 0


Query: 1   MLHNSLAIFSYLLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRS 60
           MLHNSLAIFSYLLVF+AITSTAQLPPVLDTDGQPL+RGVEYYIKPAIT+VAGNLTLK+RS
Sbjct: 1   MLHNSLAIFSYLLVFLAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITNVAGNLTLKNRS 60

Query: 61  NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE 120
           NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE
Sbjct: 61  NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE 120

Query: 121 MESATGRRFVGVGNDNSPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180
           MESATGRRFVGVGNDN PTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA
Sbjct: 121 MESATGRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 181 LDGNAFPFEFVKA 193
           LDGNAFPFEFVKA
Sbjct: 181 LDGNAFPFEFVKA 193

[Close]

BLAST of Cp4.1LG11g09390 vs. ExPASy TrEMBL
Match: A0A5A7VDV8 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00530 PE=3 SV=1)

HSP 1 Score: 310 bits (795), Expect = 1.21e-105
Identity = 147/193 (76.17%), Postives = 165/193 (85.49%), Query Frame = 0


Query: 1   MLHNSLAIFSYLLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRS 60
           MLH SLAIF Y  +FMAITSTAQ PPVLDT+GQPL+RGVEYYI PAITDV GNLTLKSRS
Sbjct: 1   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS 60

Query: 61  NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE 120
           NAPCPL+VGQEPV STNIGLPVTF P  AG+DII+E    N+ F+A STC TSTQWRVD 
Sbjct: 61  NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA 120

Query: 121 MESATGRRFVGVGNDNSPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180
            ES TGRRFVG+G+++ P GIF I R+NG YNIVWCP M+GRPRCG AGILVE+GVRL+A
Sbjct: 121 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 180

Query: 181 LDGNAFPFEFVKA 193
           LDG+AFPFEF+KA
Sbjct: 181 LDGDAFPFEFIKA 193

[Close]

BLAST of Cp4.1LG11g09390 vs. ExPASy TrEMBL
Match: A0A5D3D468 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520 PE=3 SV=1)

HSP 1 Score: 310 bits (795), Expect = 1.30e-105
Identity = 147/193 (76.17%), Postives = 165/193 (85.49%), Query Frame = 0


Query: 1   MLHNSLAIFSYLLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRS 60
           MLH SLAIF Y  +FMAITSTAQ PPVLDT+GQPL+RGVEYYI PAITDV GNLTLKSRS
Sbjct: 3   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS 62

Query: 61  NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE 120
           NAPCPL+VGQEPV STNIGLPVTF P  AG+DII+E    N+ F+A STC TSTQWRVD 
Sbjct: 63  NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA 122

Query: 121 MESATGRRFVGVGNDNSPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180
            ES TGRRFVG+G+++ P GIF I R+NG YNIVWCP M+GRPRCG AGILVE+GVRL+A
Sbjct: 123 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 182

Query: 181 LDGNAFPFEFVKA 193
           LDG+AFPFEF+KA
Sbjct: 183 LDGDAFPFEFIKA 195

[Close]

BLAST of Cp4.1LG11g09390 vs. ExPASy TrEMBL
Match: A0A6J1DB43 (miraculin-like OS=Momordica charantia OX=3673 GN=LOC111018852 PE=3 SV=1)

HSP 1 Score: 305 bits (781), Expect = 1.89e-103
Identity = 156/195 (80.00%), Postives = 167/195 (85.64%), Query Frame = 0


Query: 2   LHNSLAIFSYL-LVFMAITS-TAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSR 61
           LH SLAI S L L+FMAI+S TAQ PPVLDT+GQ L+RG+EYYIKPAITDVAGNLTLKSR
Sbjct: 3   LHKSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSR 62

Query: 62  SNAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVD 121
           S APCPLYVGQEPVAS  IGLPV FTP  A EDII E M F V FQASSTCI  TQWRV+
Sbjct: 63  SRAPCPLYVGQEPVASMEIGLPVIFTPYAADEDIIREGMDFKVVFQASSTCIMLTQWRVE 122

Query: 122 EMESATGRRFVGVGNDNSPTGIFRIDRNN-GVYNIVWCPEMVGRPRCGSAGILVEDGVRL 181
           E E+ATGRR VG G+DN PTG+FRIDR+N GVYNI WCPEM GRPRCGSAGILVEDGVRL
Sbjct: 123 ETEAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRL 182

Query: 182 LALDGNAFPFEFVKA 193
           LALDG+AFPFEFVKA
Sbjct: 183 LALDGSAFPFEFVKA 197

[Close]

BLAST of Cp4.1LG11g09390 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 68.6 bits (166), Expect = 6.5e-12
Identity = 54/196 (27.55%), Postives = 89/196 (45.41%), Query Frame = 0


Query: 5   SLAIFSYLLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRSNAPC 64
           +L + S +    +         VLD  G P+Q  V+YYI PA     G L   +R+ +  
Sbjct: 10  TLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRNLSTQ 69

Query: 65  PLYVGQEPVASTN---IGLPVTFTPIVAGEDIIEESMGFNVAFQASS-TCITSTQWRVDE 124
            L +  + V S++    GLPVTF+P+      ++ S   N+ F ++   C  S  WR+D 
Sbjct: 70  DLCLNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRIDH 129

Query: 125 MESATGRRFVGVGNDNSP-TGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVED-GVRL 184
                 + FV +G         F+I  +   Y +++CP +     C +  + ++D GVR 
Sbjct: 130 SVQLR-KSFVSIGGQKGKGNSWFQIQEDGDAYKLMYCP-ISSIVACINVSLEIDDHGVRR 189

Query: 185 LALD-GNAFPFEFVKA 194
           L L    +F  +F KA
Sbjct: 190 LVLSTDQSFVVKFQKA 203

[Close]

BLAST of Cp4.1LG11g09390 vs. TAIR 10
Match: AT1G72290.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 45.1 bits (105), Expect = 7.7e-05
Identity = 40/139 (28.78%), Postives = 62/139 (44.60%), Query Frame = 0


Query: 1   MLHNSLAIFSYLLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDV--AGNLTLKS 60
           M + S+  F  +L+F A   T    PV DT G PL    +Y+I+P  T+    G L   +
Sbjct: 1   MKNPSVISFLIILLFAATICTHGNEPVKDTAGNPLNTREQYFIQPVKTESKNGGGLVPAA 60

Query: 61  RSNAP-CPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCIT---ST 120
            +  P CPL + Q  +     GLPV+F   +     +  S   N+ F+++        S 
Sbjct: 61  ITVLPFCPLGITQ-TLLPYQPGLPVSFVLALGVGSTVMTSSAVNIEFKSNIWPFCKEFSK 120

Query: 121 QWRVDEMESATGRRFVGVG 134
            W VD+  SA     + +G
Sbjct: 121 FWEVDDSSSAPKEPSILIG 138

The following BLAST results are available for this feature:

BLAST of Cp4.1LG11g09390 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
G7LCV7	1.1e-29	39.20	Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ...	[more]
A0A072TH68	1.5e-29	39.71	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]
G7LCV1	3.3e-29	39.20	Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ...	[more]
P13087	3.1e-19	34.43	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
P07596	7.2e-16	35.95	Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2	[more]

BLAST of Cp4.1LG11g09390 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023545477.1	1.21e-137	100.00	miraculin-like [Cucurbita pepo subsp. pepo]	[more]
XP_022946294.1	4.72e-135	97.41	miraculin-like [Cucurbita moschata] >XP_022946295.1 miraculin-like [Cucurbita mo...	[more]
XP_022999136.1	9.52e-135	97.41	miraculin-like [Cucurbita maxima]	[more]
KAG7030262.1	1.59e-125	97.75	hypothetical protein SDJN02_08609, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6599263.1	9.21e-125	96.63	hypothetical protein SDJN03_09041, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Cp4.1LG11g09390 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G3G3	2.28e-135	97.41	miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1	[more]
A0A6J1KC85	4.61e-135	97.41	miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1	[more]
A0A5A7VDV8	1.21e-105	76.17	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00530...	[more]
A0A5D3D468	1.30e-105	76.17	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520...	[more]
A0A6J1DB43	1.89e-103	80.00	miraculin-like OS=Momordica charantia OX=3673 GN=LOC111018852 PE=3 SV=1	[more]

BLAST of Cp4.1LG11g09390 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73325.1	6.5e-12	27.55	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G72290.1	7.7e-05	28.78	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 26..193 e-value: 1.5E-31 score: 120.8
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 27..192 e-value: 2.0E-42 score: 145.1
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 4..192
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 27..43
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 26..192 e-value: 1.00386E-40 score: 134.007
None	No IPR available	GENE3D	2.80.10.50		coord: 22..193 e-value: 2.0E-47 score: 163.1
None	No IPR available	PANTHER	PTHR33107:SF51	PUTATIVE-RELATED	coord: 4..192
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 25..193

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG11g09390.1	Cp4.1LG11g09390.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0007165	signal transduction
molecular_function	GO:0043531	ADP binding
molecular_function	GO:0004866	endopeptidase inhibitor activity
molecular_function	GO:0003953	NAD+ nucleosidase activity