Cp4.1LG11g08700 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG11g08700
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	Cp4.1LG11: 7015764 .. 7016814 (-)
RNA-Seq Expression	Cp4.1LG11g08700
Synteny	Cp4.1LG11g08700

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GTATATTCAAGAGCTTATGCCATCGACAACCGATCCTGCATTGAAGAATGACCTTATAGTTTGTGAAAACGCATACACCATAGTGAGTAAAGCATTTCAAGAAGGAATTGAGTTATTCTTCAAAAAGGATTATAGAAGCATGTTGACTGCTGATAAGATTGCACCGAGAGCACAGGAGAGTTGCAGCGTGATTTTCAACACACCNGAGTATTTCAAAGAATCCTTATTAAGTGCCAATGCAAGCAATACACTGTTAGGGTATGGAGTCCGATACTTATTTATAGCTTGGTCAACTGTTATATCCGGTAAAACTATATTTTGTAAAGTTATATATAGTAAAGCTATATATGGTAAAGTTATATCCGCTATATATGGTAAATAGCTATATATAGTAGATGGCTAAATCTGGTAAAGCTATATATATAATAAACTATACCATATATATATATCGGGTCCGGTAGAGGTTTGAAAATGTACTTTTCTATTCTCTGTACTCTCTTGTTGTACATGCATGAAGTCATCCATAAAAAGAAAAATCTTTTTAGCCAGAGTTCTCCTTGCAAATTTCACTATCCCATTCTTTGTGTTCATCTAGATCGAGCGTGTGCGTTGTTGTGCGATCCTAACAATTGGTATCAGAGCTAACAACTGGTATCGATCCTAACATACACTTTGGTATTATTGTTATAACTACTCTAAAATTTTAAACGCGTGTTTAAGTTGATATGTTACGATAAATACTTGTATTCTTAATAGGTATATTCAAGAGCTTATGCCATCGACAACCGATCCTGCATTGAAGAATGACCTTATAGTTTGTGAAAACGCATACACCATAGTGAGTAAAGCATTTCAAGAAGGAATTGAGTTATTCTTCAAAAAGGATTATAGAAGCATGTTGACTGCTGATAAGATTGCACCGAGAGCACAGGAGAGTTGCAGCGTGATTTTCAACACACCGCCCCAGAAACAAAGCCCATTAGAGGAGCGGAATAGGGAGATGAGGATTCTCATTGCCATGGCTATAGTTTCGGGCAACATCATTGCCTTT

mRNA sequence

GTATATTCAAGAGCTTATGCCATCGACAACCGATCCTGCATTGAAGAATGACCTTATAGTTTGTGAAAACGCATACACCATAGTGAGTAAAGCATTTCAAGAAGGAATTGAGTTATTCTTCAAAAAGGATTATAGAAGCATGTTGACTGCTGATAAGATTGCACCGAGAGCACAGGAGAGTTGCAGCCTTATGCCATCGACAACCGATCCTGCATTGAAGAATGACCTTATAGTTTGTGAAAACGCATACACCATAGTGAGTAAAGCATTTCAAGAAGGAATTGAGTTATTCTTCAAAAAGGATTATAGAAGCATGTTGACTGCTGATAAGATTGCACCGAGAGCACAGGAGAGTTGCAGCGTGATTTTCAACACACCGCCCCAGAAACAAAGCCCATTAGAGGAGCGGAATAGGGAGATGAGGATTCTCATTGCCATGGCTATAGTTTCGGGCAACATCATTGCCTTT

Coding sequence (CDS)

ATGCCATCGACAACCGATCCTGCATTGAAGAATGACCTTATAGTTTGTGAAAACGCATACACCATAGTGAGTAAAGCATTTCAAGAAGGAATTGAGTTATTCTTCAAAAAGGATTATAGAAGCATGTTGACTGCTGATAAGATTGCACCGAGAGCACAGGAGAGTTGCAGCCTTATGCCATCGACAACCGATCCTGCATTGAAGAATGACCTTATAGTTTGTGAAAACGCATACACCATAGTGAGTAAAGCATTTCAAGAAGGAATTGAGTTATTCTTCAAAAAGGATTATAGAAGCATGTTGACTGCTGATAAGATTGCACCGAGAGCACAGGAGAGTTGCAGCGTGATTTTCAACACACCGCCCCAGAAACAAAGCCCATTAGAGGAGCGGAATAGGGAGATGAGGATTCTCATTGCCATGGCTATAGTTTCGGGCAACATCATTGCCTTT

Protein sequence

MPSTTDPALKNDLIVCENAYTIVSKAFQEGIELFFKKDYRSMLTADKIAPRAQESCSLMPSTTDPALKNDLIVCENAYTIVSKAFQEGIELFFKKDYRSMLTADKIAPRAQESCSVIFNTPPQKQSPLEERNREMRILIAMAIVSGNIIAF

Homology

[Close]

BLAST of Cp4.1LG11g08700 vs. NCBI nr
Match: KAG7029966.1 (hypothetical protein SDJN02_08310, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 201 bits (512), Expect = 3.38e-62
Identity = 117/227 (51.54%), Postives = 133/227 (58.59%), Query Frame = 0


Query: 1   MPSTTDPALKNDLIVCENAYTIVSKAFQEGIELFFKKDYRSMLTADKIAPRAQES----- 60
           +PSTTDPALKNDLIVCENAY  +S+AFQEGIELFFKKDYRSML A+K+APRAQ +     
Sbjct: 17  LPSTTDPALKNDLIVCENAYKTLSEAFQEGIELFFKKDYRSMLNAEKVAPRAQATTRETK 76

Query: 61  -------------------------------------CS--------------------- 120
                                                CS                     
Sbjct: 77  PTRGEEQGDEDSHCLVGDSDTEDRINNLCHQMEEFGFCSQTFHENLNGPTDDIGLTQIAI 136

Query: 121 ----------------LMPSTTDPALKNDLIVCENAYTIVSKAFQEGIELFFKKDYRSML 148
                           L+P+ TDPALKN LIVCENAY  V++A QEGIELFFKKDYRSML
Sbjct: 137 FQAQANASKTLRYIQELLPTITDPALKNGLIVCENAYKTVNEALQEGIELFFKKDYRSML 196

[Close]

BLAST of Cp4.1LG11g08700 vs. NCBI nr
Match: XP_022972818.1 (uncharacterized protein LOC111471342 [Cucurbita maxima])

HSP 1 Score: 145 bits (365), Expect = 7.87e-41
Identity = 70/91 (76.92%), Postives = 81/91 (89.01%), Query Frame = 0


Query: 58  LMPSTTDPALKNDLIVCENAYTIVSKAFQEGIELFFKKDYRSMLTADKIAPRAQESCSVI 117
           L+P+ TDPALKN+L+VCENAY  V++A QEGIELFFKKDYRSML  +KIAPRAQ SC++I
Sbjct: 84  LLPTITDPALKNNLLVCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCNMI 143

Query: 118 FNTPPQKQSPLEERNREMRILIAMAIVSGNI 148
           FNTPP+KQ  LEERNRE+RILIAMAIVSG I
Sbjct: 144 FNTPPEKQGLLEERNREIRILIAMAIVSGYI 174

[Close]

BLAST of Cp4.1LG11g08700 vs. NCBI nr
Match: KAG6599000.1 (hypothetical protein SDJN03_08778, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 144 bits (362), Expect = 1.82e-40
Identity = 74/110 (67.27%), Postives = 87/110 (79.09%), Query Frame = 0


Query: 39  YRSMLTADKIAPRAQESCSLMPSTTDPALKNDLIVCENAYTIVSKAFQEGIELFFKKDYR 98
           +++   A K     QE   L+P+ TDPALKN LIVCENAY  V++A QEGIELFFKKDYR
Sbjct: 61  FQAQANASKTLRYIQE---LLPTITDPALKNGLIVCENAYKTVNEALQEGIELFFKKDYR 120

Query: 99  SMLTADKIAPRAQESCSVIFNTPPQKQSPLEERNREMRILIAMAIVSGNI 148
           SML  +KIAPRAQ SC++IF+TPP+KQ  LEERNRE+RILIAMAIVSG I
Sbjct: 121 SMLNVEKIAPRAQASCNMIFSTPPEKQGLLEERNREIRILIAMAIVSGYI 167

[Close]

BLAST of Cp4.1LG11g08700 vs. NCBI nr
Match: XP_022946902.1 (uncharacterized protein LOC111450843 [Cucurbita moschata])

HSP 1 Score: 144 bits (362), Expect = 2.52e-40
Identity = 74/110 (67.27%), Postives = 87/110 (79.09%), Query Frame = 0


Query: 39  YRSMLTADKIAPRAQESCSLMPSTTDPALKNDLIVCENAYTIVSKAFQEGIELFFKKDYR 98
           +++   A K     QE   L+P+ TDPALKN LIVCENAY  V++A QEGIELFFKKDYR
Sbjct: 72  FQAQANASKTLRYIQE---LLPTITDPALKNGLIVCENAYKTVNEALQEGIELFFKKDYR 131

Query: 99  SMLTADKIAPRAQESCSVIFNTPPQKQSPLEERNREMRILIAMAIVSGNI 148
           SML  +KIAPRAQ SC++IF+TPP+KQ  LEERNRE+RILIAMAIVSG I
Sbjct: 132 SMLNVEKIAPRAQASCNMIFSTPPEKQGLLEERNREIRILIAMAIVSGYI 178

[Close]

BLAST of Cp4.1LG11g08700 vs. NCBI nr
Match: XP_022156377.1 (uncharacterized protein LOC111023283 [Momordica charantia])

HSP 1 Score: 134 bits (337), Expect = 1.35e-36
Identity = 64/93 (68.82%), Postives = 80/93 (86.02%), Query Frame = 0


Query: 58  LMPSTTDPALKNDLIVCENAYTIVSKAFQEGIELFFKKDYRSMLTADKIAPRAQESCSVI 117
           L+   +DPALKN+LIVCENAY I++KAF EGI+ FF++DYRSM+ A++IAPRA+ SCSVI
Sbjct: 88  LLAFVSDPALKNNLIVCENAYDIMNKAFLEGIKYFFQRDYRSMVNAERIAPRAEASCSVI 147

Query: 118 FNTPPQKQSPLEERNREMRILIAMAIVSGNIIA 150
           F TPP++ SPL ERNREMRILIAMA+VS + IA
Sbjct: 148 FGTPPERSSPLVERNREMRILIAMALVSASYIA 180

[Close]

BLAST of Cp4.1LG11g08700 vs. ExPASy TrEMBL
Match: A0A6J1IB75 (uncharacterized protein LOC111471342 OS=Cucurbita maxima OX=3661 GN=LOC111471342 PE=4 SV=1)

HSP 1 Score: 145 bits (365), Expect = 3.81e-41
Identity = 70/91 (76.92%), Postives = 81/91 (89.01%), Query Frame = 0


Query: 58  LMPSTTDPALKNDLIVCENAYTIVSKAFQEGIELFFKKDYRSMLTADKIAPRAQESCSVI 117
           L+P+ TDPALKN+L+VCENAY  V++A QEGIELFFKKDYRSML  +KIAPRAQ SC++I
Sbjct: 84  LLPTITDPALKNNLLVCENAYKTVNEALQEGIELFFKKDYRSMLNVEKIAPRAQASCNMI 143

Query: 118 FNTPPQKQSPLEERNREMRILIAMAIVSGNI 148
           FNTPP+KQ  LEERNRE+RILIAMAIVSG I
Sbjct: 144 FNTPPEKQGLLEERNREIRILIAMAIVSGYI 174

[Close]

BLAST of Cp4.1LG11g08700 vs. ExPASy TrEMBL
Match: A0A6J1G4X2 (uncharacterized protein LOC111450843 OS=Cucurbita moschata OX=3662 GN=LOC111450843 PE=4 SV=1)

HSP 1 Score: 144 bits (362), Expect = 1.22e-40
Identity = 74/110 (67.27%), Postives = 87/110 (79.09%), Query Frame = 0


Query: 39  YRSMLTADKIAPRAQESCSLMPSTTDPALKNDLIVCENAYTIVSKAFQEGIELFFKKDYR 98
           +++   A K     QE   L+P+ TDPALKN LIVCENAY  V++A QEGIELFFKKDYR
Sbjct: 72  FQAQANASKTLRYIQE---LLPTITDPALKNGLIVCENAYKTVNEALQEGIELFFKKDYR 131

Query: 99  SMLTADKIAPRAQESCSVIFNTPPQKQSPLEERNREMRILIAMAIVSGNI 148
           SML  +KIAPRAQ SC++IF+TPP+KQ  LEERNRE+RILIAMAIVSG I
Sbjct: 132 SMLNVEKIAPRAQASCNMIFSTPPEKQGLLEERNREIRILIAMAIVSGYI 178

[Close]

BLAST of Cp4.1LG11g08700 vs. ExPASy TrEMBL
Match: A0A6J1DRX6 (uncharacterized protein LOC111023283 OS=Momordica charantia OX=3673 GN=LOC111023283 PE=4 SV=1)

HSP 1 Score: 134 bits (337), Expect = 6.56e-37
Identity = 64/93 (68.82%), Postives = 80/93 (86.02%), Query Frame = 0


Query: 58  LMPSTTDPALKNDLIVCENAYTIVSKAFQEGIELFFKKDYRSMLTADKIAPRAQESCSVI 117
           L+   +DPALKN+LIVCENAY I++KAF EGI+ FF++DYRSM+ A++IAPRA+ SCSVI
Sbjct: 88  LLAFVSDPALKNNLIVCENAYDIMNKAFLEGIKYFFQRDYRSMVNAERIAPRAEASCSVI 147

Query: 118 FNTPPQKQSPLEERNREMRILIAMAIVSGNIIA 150
           F TPP++ SPL ERNREMRILIAMA+VS + IA
Sbjct: 148 FGTPPERSSPLVERNREMRILIAMALVSASYIA 180

[Close]

BLAST of Cp4.1LG11g08700 vs. ExPASy TrEMBL
Match: A0A6J1KFT2 (uncharacterized protein LOC111493503 OS=Cucurbita maxima OX=3661 GN=LOC111493503 PE=4 SV=1)

HSP 1 Score: 128 bits (322), Expect = 5.72e-35
Identity = 64/92 (69.57%), Postives = 75/92 (81.52%), Query Frame = 0


Query: 58  LMPSTTDPALKNDLIVCENAYTIVSKAFQEGIELFFKKDYRSMLTADKIAPRAQESCSVI 117
           L+P+ TD  LKNDLI+CENAY  V +A QEGI LFFKKDYR ML A KIAPRAQ SC +I
Sbjct: 62  LLPTITDLKLKNDLIICENAYKTVDEALQEGIGLFFKKDYRGMLIAVKIAPRAQASCDMI 121

Query: 118 FNTPPQKQSPLEERNREMRILIAMAIVSGNII 149
           F+TP +K+  LEERNRE+RIL+AMAIV G+II
Sbjct: 122 FSTPVKKRGLLEERNREIRILVAMAIVCGSII 153

[Close]

BLAST of Cp4.1LG11g08700 vs. ExPASy TrEMBL
Match: A0A1S3CCT5 (putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103499372 PE=4 SV=1)

HSP 1 Score: 125 bits (313), Expect = 1.39e-33
Identity = 61/91 (67.03%), Postives = 75/91 (82.42%), Query Frame = 0


Query: 58  LMPSTTDPALKNDLIVCENAYTIVSKAFQEGIELFFKKDYRSMLTADKIAPRAQESCSVI 117
           L+ STTDPALKNDLIVCENA+ IV +AF E + LF ++DYR ML+A++  PRAQ SC VI
Sbjct: 64  LLRSTTDPALKNDLIVCENAFRIVKQAFGEALRLFSQRDYRGMLSAERPTPRAQASCDVI 123

Query: 118 FNTPPQKQSPL-EERNREMRILIAMAIVSGN 147
           F+TPP + SP+ +ERNREMRILIAMAI SG+
Sbjct: 124 FSTPPARHSPIMDERNREMRILIAMAITSGS 154

[Close]

BLAST of Cp4.1LG11g08700 vs. TAIR 10
Match: AT1G09370.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 49.7 bits (117), Expect = 2.4e-06
Identity = 24/81 (29.63%), Postives = 44/81 (54.32%), Query Frame = 0


Query: 70  DLIVCENAYTIVSKAFQEGIELFFKKDYRSMLTADKIAPRAQESCSVIFNTPPQKQSPLE 129
           DL  C  AY IV+ AF + +  F +  Y  ++  +K    A + C   FN    + +PL 
Sbjct: 93  DLQACAEAYAIVNSAFTKAVTFFKQAYYSKIVNIEKKVSMAVDICKTDFNVLGYQINPLI 152

Query: 130 ERNREMRILIAMAIVSGNIIA 151
           E+NR+ +IL++M  +  ++++
Sbjct: 153 EKNRQTKILLSMEQIVSHMVS 173

[Close]

BLAST of Cp4.1LG11g08700 vs. TAIR 10
Match: AT1G09360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 45.4 bits (106), Expect = 4.6e-05
Identity = 31/85 (36.47%), Postives = 42/85 (49.41%), Query Frame = 0


Query: 65  PALKNDLIVCENAYTIVSKAFQEGIELFFKKDYRSMLTADKIAPRAQESCSVIFNTPPQK 124
           P   + L  C   Y   + +F E   LF K +Y  M  A     +  E C   F  PP K
Sbjct: 86  PKETSGLKTCHAVYKRETNSFLEIRFLFSKGEYELMDEAILSTAKILEECRGDFLIPPYK 145

Query: 125 QSPLEERNREMRILIAMAIVSGNII 150
           + PL E+ R MRILI M+ VSG+++
Sbjct: 146 E-PLLEKKRVMRILITMSAVSGHMV 169

The following BLAST results are available for this feature:

BLAST of Cp4.1LG11g08700 vs.


Match Name	E-value	Identity	Description

BLAST of Cp4.1LG11g08700 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7029966.1	3.38e-62	51.54	hypothetical protein SDJN02_08310, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022972818.1	7.87e-41	76.92	uncharacterized protein LOC111471342 [Cucurbita maxima]	[more]
KAG6599000.1	1.82e-40	67.27	hypothetical protein SDJN03_08778, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022946902.1	2.52e-40	67.27	uncharacterized protein LOC111450843 [Cucurbita moschata]	[more]
XP_022156377.1	1.35e-36	68.82	uncharacterized protein LOC111023283 [Momordica charantia]	[more]

BLAST of Cp4.1LG11g08700 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IB75	3.81e-41	76.92	uncharacterized protein LOC111471342 OS=Cucurbita maxima OX=3661 GN=LOC111471342...	[more]
A0A6J1G4X2	1.22e-40	67.27	uncharacterized protein LOC111450843 OS=Cucurbita moschata OX=3662 GN=LOC1114508...	[more]
A0A6J1DRX6	6.56e-37	68.82	uncharacterized protein LOC111023283 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1KFT2	5.72e-35	69.57	uncharacterized protein LOC111493503 OS=Cucurbita maxima OX=3661 GN=LOC111493503...	[more]
A0A1S3CCT5	1.39e-33	67.03	putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103499372 PE=4 SV=1	[more]

BLAST of Cp4.1LG11g08700 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G09370.1	2.4e-06	29.63	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G09360.1	4.6e-05	36.47	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 52..146 e-value: 2.2E-6 score: 29.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 58..144
None	No IPR available	PANTHER	PTHR36710:SF1	F14J9.2 PROTEIN	coord: 61..149
None	No IPR available	PANTHER	PTHR36710	PECTINESTERASE INHIBITOR-LIKE	coord: 61..149
None	No IPR available	PANTHER	PTHR36710	PECTINESTERASE INHIBITOR-LIKE	coord: 4..57
None	No IPR available	PANTHER	PTHR36710:SF1	F14J9.2 PROTEIN	coord: 4..57
IPR034086	Pectinesterase inhibitor, plant	CDD	cd15797	PMEI	coord: 57..149 e-value: 3.39623E-20 score: 79.3885

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG11g08700.1	Cp4.1LG11g08700.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0046910	pectinesterase inhibitor activity