Cp4.1LG11g08570 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG11g08570
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionWAT1-related protein
LocationCp4.1LG11: 6998686 .. 7001465 (+)
RNA-Seq ExpressionCp4.1LG11g08570
SyntenyCp4.1LG11g08570
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTCTCATGGATGGATTCATTCTATGAATAAACCTCTGTATCTCTCTCTCTCTAAGAAAGAAGAAGAAGAATCATGGAGGGAGATATCACAGCAATCATGGGGATGGTTATATTGCAAGTTTGCTACGCTGGTTTAAGCATTACCTCAAAGCTAGCCATGCAATCAGGAATGAACCCTCTTGTTCTTCTCACTTACAGGCAATTTTTTGGCACTTTGGCCATTGCTCCCTTTGCTTTCTTTACTGAAAGGTACTCAAAATGAATGAAAAGATCATCAATTTCTGGGTTTGAAAAGATGATCAAGAACAATGAATGAATCTTTCTTGGTTCTTGTTCTATGTTTTGTTTCTTTGGCAGGAAAACAAGGCCAAAGATCACTTTCCCTGTTCTTGTTCAAATCTTTATCTGTTCTTTATCTGGGTGAGTCAAATGGGTTGTGATCAAGTTTTTAAATTTTGTAACTTTTTTAGAATTGAAAGGAATTCTATGAATGTTTGTGCAGAGCCACAGCGAACCAGATATTCTTCTTTGTTGGGTTGAAATGCACAAACCCAACCGTTTCAAGTGCTATGGCTAATGTTCTTCCTGCTGCTACTTTTATTCTTGCTGTTGTTTTCAGGTTAGATTTTCAAAGCTGCCATATGTCTGCTTTGGCTTGTTACGTATTGATGTTAGCCTCATGGTTTTAAAATGTGTCTACTATGGAGAGGTATTGTGAGATCTCATATTGGTTGGAGAGGGGAACAAAGGTGTGGAAACTAATCTCTCTGGTAGATGCTGTTTAAAATTGTGAAGCTGACGGGTATATGTAAGGAGCTAAAGTGGACAATCTCTGCTAGTGGTGGGTTTGAGGTGTTAGAAATGGTTTTAGAGCTAGACACTGGGCGGTGTGCCAGCGAGGATGTTGGGCCCTCAAGGGGGGTGGAGAGGGGAACGAAACATTCCTTATAAGCATGTGGAAACTTTTCCCTAGTAGATGCGTTTTAAAACCGTGAGGCTGATGGCGATACATAATGGGCCAAAGCGAACAATTAGCGGTGGGCTTGAGTTGTTACAAATGGTATCAGAGCCAGACATTGGGCGGTGTGCAAACGAGGACGTTGGGCCCTCAAGTGGGCTGGATTATGAGATCCCACGTCAGTTGGAGAGGGGAATGAAATATTCTTTAGAAGTATGTGGAAACTTTTCCCTAGTAGACGCGTTTTAAAACCGTGAGGCTGACGACGATACGTATCGGGCTAAAATGGACAATATCGGCTAGTAGTGGGGTTTGGCTGTTACAAACGGTATCGGAGTCAGATATTGAGCGGTGTGCCACAAAGACGTTGGGCCTTCAAGGGGGGTGGATTGTGAGATCCCACATCGGTTGGAGAGGGGAACGAAACATTCCTTATAAGCGTGTGGAAACCTCTCTCTAGTAGATACGTTTTAAAACCGTGAGGCTAACGATGATACGTAACGGGCCAAAACGGACAATATCTACTAGCAGTAGACTCAGGCTGTTACATCTATCGAAAGGCCACGAGTGTTCTTCTAGGTTTTTAAGTTTACTTATCAATATATATAAATTTGTTTACGTTAATCTATCCGCTTAAGCTTTCAATTTAGCGGTAGCGTTAAGAGTAAACTTAAGGGCATGCCATTATTTTGGCAGACAAGAGAGTGTGAGAATCAAGACCAAGCCTGGTCTTGCAAAGGTGATGGGAACAATTGTGTGTATATGTGGAGCAATGCTGCTATCTTTCTACCATGGACACACCATTGGCTTAGGTGAATCAAAAATTCACTGGCCTTATGTCGAGAGGATCATGAGCAAAACAAACCCGACGAACCGTCAAGAAAACCGAACCCTCGGCTCGGTTTTGTTGCTTTGCAGCTCGTTCTCTTGGGCGTTATGGTTCGTCATTCAAGTAAGTAGCCATGACCCCTAATTTGGTCTATTTTTCAAGCCAAAGACAGCTGAAACCATTCTTTGTAGGCAAGATTGAGTGTCAAGTTCAAAGCTCCTTATACAAGTACAACGTTGTTGTGCTTCATGGCCTTTTTCCAATGTGGACTCATAGCTGTAATTTCCGAGCACAACGTCGCTTCTTGGTCATTGAAAAGTCCGATCCGACTCATCTCGGCCCTTTACACGGTACGTAACGTACTTAATAATCGCTCGATGCATGGTGTCGATATGTCGGTATGAAACTAATGGAAGTTTTCACAGGGAATTGTGTGTTCGGGATTGACATTTAGCATCACTTCTTGGATTATACAAAGGAAAGGGCCTCTCTATGTCTCAATTTTCACCCCATTGTTGCTTATCATTGTGGCCATCTTTAGCTGGGCTCTCCTCCACGAACAACTCTACGTTGGAACGTGCGTATCCATGCCACTCTCGTATATTCAAACTTCTGACACCTTTTCTATTTCGTATAGAACTGTTATAATCGTTATCAAATACTCCACGTACTCTCGTATATTCAAATTTATGAGATATTTTCTATTTCGTATAGAACTGTTTACTTATCGGGTTTTGTGCGTTCGTGATCGTTCGTTTTTAACAGTGTTCTTGGCTCGATTTTGATAATCATTGGGCTATATGGAGTGCTATGGGGAAAGTCCAAAGAGATGAAAGTAGAAGAGGAAGACAAGGTGGAAAAGGCAACAAATGGGAGTCAAAAAGATGACGTGGAATTGCAAATGAAATGATTTGGAAGATTTTTTTTTTTTTTTTTAATTTTAAGTTTGTGTATAAGTTTCTCTTTTGCAAATTTAATTCTTATGTACAATA

mRNA sequence

CTCTCTCATGGATGGATTCATTCTATGAATAAACCTCTGTATCTCTCTCTCTCTAAGAAAGAAGAAGAAGAATCATGGAGGGAGATATCACAGCAATCATGGGGATGGTTATATTGCAAGTTTGCTACGCTGGTTTAAGCATTACCTCAAAGCTAGCCATGCAATCAGGAATGAACCCTCTTGTTCTTCTCACTTACAGGCAATTTTTTGGCACTTTGGCCATTGCTCCCTTTGCTTTCTTTACTGAAAGGAAAACAAGGCCAAAGATCACTTTCCCTGTTCTTGTTCAAATCTTTATCTGTTCTTTATCTGGACAAGAGAGTGTGAGAATCAAGACCAAGCCTGGTCTTGCAAAGGTGATGGGAACAATTGTGTGTATATGTGGAGCAATGCTGCTATCTTTCTACCATGGACACACCATTGGCTTAGGTGAATCAAAAATTCACTGGCCTTATGTCGAGAGGATCATGAGCAAAACAAACCCGACGAACCGTCAAGAAAACCGAACCCTCGGCTCGGTTTTGTTGCTTTGCAGCTCGTTCTCTTGGGCGTTATGGTTCGTCATTCAAGCAAGATTGAGTGTCAAGTTCAAAGCTCCTTATACAAGTACAACGTTGTTGTGCTTCATGGCCTTTTTCCAATGTGGACTCATAGCTGTAATTTCCGAGCACAACGTCGCTTCTTGGTCATTGAAAAGTCCGATCCGACTCATCTCGGCCCTTTACACGGGAATTGTGTGTTCGGGATTGACATTTAGCATCACTTCTTGGATTATACAAAGGAAAGGGCCTCTCTATGTCTCAATTTTCACCCCATTGTTGCTTATCATTGTGGCCATCTTTAGCTGGGCTCTCCTCCACGAACAACTCTACGTTGGAACTGTTCTTGGCTCGATTTTGATAATCATTGGGCTATATGGAGTGCTATGGGGAAAGTCCAAAGAGATGAAAGTAGAAGAGGAAGACAAGGTGGAAAAGGCAACAAATGGGAGTCAAAAAGATGACGTGGAATTGCAAATGAAATGATTTGGAAGATTTTTTTTTTTTTTTTTAATTTTAAGTTTGTGTATAAGTTTCTCTTTTGCAAATTTAATTCTTATGTACAATA

Coding sequence (CDS)

ATGGAGGGAGATATCACAGCAATCATGGGGATGGTTATATTGCAAGTTTGCTACGCTGGTTTAAGCATTACCTCAAAGCTAGCCATGCAATCAGGAATGAACCCTCTTGTTCTTCTCACTTACAGGCAATTTTTTGGCACTTTGGCCATTGCTCCCTTTGCTTTCTTTACTGAAAGGAAAACAAGGCCAAAGATCACTTTCCCTGTTCTTGTTCAAATCTTTATCTGTTCTTTATCTGGACAAGAGAGTGTGAGAATCAAGACCAAGCCTGGTCTTGCAAAGGTGATGGGAACAATTGTGTGTATATGTGGAGCAATGCTGCTATCTTTCTACCATGGACACACCATTGGCTTAGGTGAATCAAAAATTCACTGGCCTTATGTCGAGAGGATCATGAGCAAAACAAACCCGACGAACCGTCAAGAAAACCGAACCCTCGGCTCGGTTTTGTTGCTTTGCAGCTCGTTCTCTTGGGCGTTATGGTTCGTCATTCAAGCAAGATTGAGTGTCAAGTTCAAAGCTCCTTATACAAGTACAACGTTGTTGTGCTTCATGGCCTTTTTCCAATGTGGACTCATAGCTGTAATTTCCGAGCACAACGTCGCTTCTTGGTCATTGAAAAGTCCGATCCGACTCATCTCGGCCCTTTACACGGGAATTGTGTGTTCGGGATTGACATTTAGCATCACTTCTTGGATTATACAAAGGAAAGGGCCTCTCTATGTCTCAATTTTCACCCCATTGTTGCTTATCATTGTGGCCATCTTTAGCTGGGCTCTCCTCCACGAACAACTCTACGTTGGAACTGTTCTTGGCTCGATTTTGATAATCATTGGGCTATATGGAGTGCTATGGGGAAAGTCCAAAGAGATGAAAGTAGAAGAGGAAGACAAGGTGGAAAAGGCAACAAATGGGAGTCAAAAAGATGACGTGGAATTGCAAATGAAATGA

Protein sequence

MEGDITAIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERKTRPKITFPVLVQIFICSLSGQESVRIKTKPGLAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNVASWSLKSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALLHEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEEEDKVEKATNGSQKDDVELQMK
Homology
BLAST of Cp4.1LG11g08570 vs. ExPASy Swiss-Prot
Match: Q8GXB4 (WAT1-related protein At1g09380 OS=Arabidopsis thaliana OX=3702 GN=At1g09380 PE=2 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 4.4e-95
Identity = 178/358 (49.72%), Postives = 239/358 (66.76%), Query Frame = 0

Query: 2   EGDITAIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERKT 61
           + D+   + MV++Q+ YAG++ITSK+AM++GM PL+L+ YRQ F T+A  P AFF ERKT
Sbjct: 3   KSDMLPFLAMVLVQIGYAGMNITSKMAMEAGMKPLILVAYRQIFATIATFPVAFFLERKT 62

Query: 62  RPKITFPVLVQIFICSLSG---------------------------------------QE 121
           RPKIT  +LVQ+F CS++G                                       QE
Sbjct: 63  RPKITLRILVQVFFCSITGATGNQVLYFVGLQNSSPTIACALTNLLPAVTFLLAAIFRQE 122

Query: 122 SVRIKTKPGLAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQE 181
           +V IK   G AKV+GT+VC+ GAM+LSFYHGHTIG+GESKIHW Y E I +K   ++   
Sbjct: 123 TVGIKKASGQAKVIGTLVCVIGAMVLSFYHGHTIGIGESKIHWAYAENI-TKHGSSSGHS 182

Query: 182 NRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNVA 241
           N  LG  L++ ++ SWA WF+IQ ++S  F APYTST L+C M   QCG IA+IS+H ++
Sbjct: 183 NFFLGPFLIMAAAVSWAAWFIIQTKMSETFAAPYTSTLLMCLMGSIQCGAIALISDHTIS 242

Query: 242 SWSLKSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALLH 301
            WSL SP+R ISALY G+V S L F + SW +QRKGPLYVS+F+PLLL++VAIFSWALL 
Sbjct: 243 DWSLSSPLRFISALYAGVVASALAFCLMSWAMQRKGPLYVSVFSPLLLVVVAIFSWALLE 302

Query: 302 EQLYVGTVLGSILIIIGLYGVLWGKSKEM--KVEEEDKVEKATN---GSQKDDVELQM 316
           E+LY GT +GS L++IGLYGVLWGK +E+  K EE +KV++  +       +D+E ++
Sbjct: 303 EKLYTGTFMGSALVVIGLYGVLWGKDREVSEKEEEREKVKQQNHKVKSESNEDIESRL 359

BLAST of Cp4.1LG11g08570 vs. ExPASy Swiss-Prot
Match: Q9LI65 (WAT1-related protein At3g30340 OS=Arabidopsis thaliana OX=3702 GN=At3g30340 PE=2 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 8.6e-51
Identity = 111/324 (34.26%), Postives = 187/324 (57.72%), Query Frame = 0

Query: 7   AIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERKTRPKIT 66
           A++ M ++ +  + +++  K  +  G+N +V  TYR   GTL + PFA F ER  RPK+T
Sbjct: 11  AVLMMSMINIGLSVVNVMFKKMIDEGLNRMVATTYRLAVGTLFLIPFAIFLERHNRPKLT 70

Query: 67  FPVLVQIFICSLSG---------------------------------------QESVRIK 126
             +L  +F  +L G                                       QE++ IK
Sbjct: 71  GRILCSLFFSALLGTSLVQYFFLIGLEYTSSTFSLAFSNMVPSVTFALALVFRQETLNIK 130

Query: 127 TKPGLAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQENR-TL 186
           +  G AK++GT++CICGA++L+ Y G  +    S+ H  ++E   ++T+ T     +  +
Sbjct: 131 SNVGRAKLLGTMICICGALVLTLYKGTAL----SREHSTHME-THTRTDSTGAMTQKWAM 190

Query: 187 GSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNVASWSL 246
           GS++L+ S   W+ WF++QA++S  +   YTSTT+L F    Q  L+++ISE + + W +
Sbjct: 191 GSIMLVISIIIWSSWFIVQAKISRVYPCQYTSTTILSFFGVIQSALLSLISERSTSMWVV 250

Query: 247 KSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALLHEQLY 291
           K   ++++ LY+GIV SGL +   SW ++++G ++ S F PL+ +  AIFS++ LHEQ+Y
Sbjct: 251 KDKFQVLALLYSGIVGSGLCYVGMSWCLRQRGAVFTSSFIPLIQVFAAIFSFSFLHEQIY 310

BLAST of Cp4.1LG11g08570 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 9.9e-47
Identity = 112/344 (32.56%), Postives = 189/344 (54.94%), Query Frame = 0

Query: 11  MVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERKTRPKITFPVL 70
           M+ LQ  YAG++I +K+++ +GM+  VL+ YR    T  IAPFAFF ERK +PKITF + 
Sbjct: 22  MISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQPKITFSIF 81

Query: 71  VQIFICSLSG---------------------------------------QESVRIKTKPG 130
           +Q+FI  L G                                        E + +K    
Sbjct: 82  MQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDLKKLWC 141

Query: 131 LAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQENRTL--GSV 190
            AK+ GT+V + GAML++ Y G  + L  +K          + T+  N   ++    GS+
Sbjct: 142 QAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSSSDKEFLKGSI 201

Query: 191 LLLCSSFSWALWFVIQAR-LSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNVASWSLKS 250
           LL+ ++ +WA  FV+QA+ L    K   + TTL+CF+   Q   +  + EHN ++W +  
Sbjct: 202 LLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHNPSAWRIGW 261

Query: 251 PIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALLHEQLYVG 310
            + L++A Y+GIV S +++ +   +++++GP++ + F+PL+++IVA+    +L E++++G
Sbjct: 262 DMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFVLAEKIFLG 321

Query: 311 TVLGSILIIIGLYGVLWGKSKEMKVEEEDKVEKATNGSQKDDVE 313
            V+G++LI+IGLY VLWGK KE +V   +  +  +N    +DVE
Sbjct: 322 GVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSKVTEDVE 365

BLAST of Cp4.1LG11g08570 vs. ExPASy Swiss-Prot
Match: F4HVM3 (WAT1-related protein At1g68170 OS=Arabidopsis thaliana OX=3702 GN=At1g68170 PE=3 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 4.9e-46
Identity = 118/345 (34.20%), Postives = 184/345 (53.33%), Query Frame = 0

Query: 4   DITAIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERKTRP 63
           DITA   MV++Q+  AGL+I  KLAM+ GMNP VL+ YR  F TL + P  F  +RK RP
Sbjct: 3   DITA---MVVVQIATAGLNIFFKLAMEDGMNPSVLVAYRLLFATLFMIPICFIFQRKKRP 62

Query: 64  KITFPVLVQIFICSLSG---------------------------------------QESV 123
           + T  +++   +  L G                                        ESV
Sbjct: 63  EFTCRLMLLALLSGLLGVVIPSILTITGLALTSATFTSAAGVLTPLVTFIFAALLRMESV 122

Query: 124 RIKTKPGLAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQENR 183
           R+ +  GLAKV GT+  + GA++  FY G  I L  + ++     R  S+   T+     
Sbjct: 123 RLGSSVGLAKVFGTLFGVGGALVFIFYRGIEIRLWSTHVNLVNQPRDSSRDATTHHIS-- 182

Query: 184 TLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNVASW 243
            LG++L+   + S +LWF++Q ++S +F  PY + TL+  M      L+A+  EH++  W
Sbjct: 183 ILGALLVFGGNISISLWFLLQVKISKQFGGPYWNATLMNMMGGVVAMLVALCWEHDLDEW 242

Query: 244 SLKSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALLHEQ 303
            L   IRL++  Y  I+ SG+  ++ +W I+ +GPL+VS+F+P+ L+IVA+    LL E 
Sbjct: 243 RLGWNIRLLTIAYAAILISGMVVAVNAWCIESRGPLFVSVFSPVGLVIVALVGSFLLDET 302

Query: 304 LYVGTVLGSILIIIGLYGVLWGKSKEMKVEEEDKVEKATNGSQKD 310
           L++G+++G+++I+  LY VLW K+KEMK          TN + KD
Sbjct: 303 LHLGSIIGTVIIVGALYIVLWAKNKEMKSMLTTSDHNETNKTSKD 342

BLAST of Cp4.1LG11g08570 vs. ExPASy Swiss-Prot
Match: Q9FNA5 (WAT1-related protein At5g13670 OS=Arabidopsis thaliana OX=3702 GN=At5g13670 PE=2 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.4e-45
Identity = 112/327 (34.25%), Postives = 180/327 (55.05%), Query Frame = 0

Query: 9   MGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERKTRPKITFP 68
           + +V +Q  YA +SI +KLA+  GM+P VL+ YR    +  I PFA   ER TRPK+TF 
Sbjct: 10  IAIVFIQCLYALMSIVAKLALNKGMSPHVLVAYRMAVASALITPFALILERNTRPKLTFK 69

Query: 69  VLVQIFICSL-----------SGQ----------------------------ESVRIKTK 128
           +L+QI I SL           SG                             E V I+ +
Sbjct: 70  ILLQIAILSLFEPVVEQNLYYSGMKLTTATFTSALCNALPAMTFIMACVFKLEKVTIERR 129

Query: 129 PGLAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTN-----RQENR 188
              AK++GT+V I GAML++F  G+ I L       P+          T+     +Q + 
Sbjct: 130 HSQAKLVGTMVAIGGAMLMTFVKGNVIEL-------PWTSNSRGLNGHTHAMRIPKQADI 189

Query: 189 TLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEH-NVAS 248
             GS++L+ S FSW+ + ++QA++  ++KA  + T L+C M   +  ++ +I E  N++ 
Sbjct: 190 ARGSIMLVASCFSWSCYIILQAKILAQYKAELSLTALMCIMGMLEATVMGLIWERKNMSV 249

Query: 249 WSLKSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALLHE 291
           W +   + L++++Y G+V SGL + +  W  + +GP++VS F PL +++VAI S  +  E
Sbjct: 250 WKINPDVTLLASIYGGLV-SGLAYYVIGWASKERGPVFVSAFNPLSMVLVAILSTFVFLE 309

BLAST of Cp4.1LG11g08570 vs. NCBI nr
Match: XP_023545290.1 (WAT1-related protein At1g09380 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 617 bits (1592), Expect = 1.04e-222
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 1   MEGDITAIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERK 60
           MEGDITAIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERK
Sbjct: 1   MEGDITAIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERK 60

Query: 61  TRPKITFPVLVQIFICSLSGQESVRIKTKPGLAKVMGTIVCICGAMLLSFYHGHTIGLGE 120
           TRPKITFPVLVQIFICSLSGQESVRIKTKPGLAKVMGTIVCICGAMLLSFYHGHTIGLGE
Sbjct: 61  TRPKITFPVLVQIFICSLSGQESVRIKTKPGLAKVMGTIVCICGAMLLSFYHGHTIGLGE 120

Query: 121 SKIHWPYVERIMSKTNPTNRQENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTT 180
           SKIHWPYVERIMSKTNPTNRQENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTT
Sbjct: 121 SKIHWPYVERIMSKTNPTNRQENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTT 180

Query: 181 LLCFMAFFQCGLIAVISEHNVASWSLKSPIRLISALYTGIVCSGLTFSITSWIIQRKGPL 240
           LLCFMAFFQCGLIAVISEHNVASWSLKSPIRLISALYTGIVCSGLTFSITSWIIQRKGPL
Sbjct: 181 LLCFMAFFQCGLIAVISEHNVASWSLKSPIRLISALYTGIVCSGLTFSITSWIIQRKGPL 240

Query: 241 YVSIFTPLLLIIVAIFSWALLHEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEEEDKVE 300
           YVSIFTPLLLIIVAIFSWALLHEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEEEDKVE
Sbjct: 241 YVSIFTPLLLIIVAIFSWALLHEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEEEDKVE 300

Query: 301 KATNGSQKDDVELQMK 316
           KATNGSQKDDVELQMK
Sbjct: 301 KATNGSQKDDVELQMK 316

BLAST of Cp4.1LG11g08570 vs. NCBI nr
Match: XP_023545289.1 (WAT1-related protein At1g09380 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 598 bits (1542), Expect = 1.84e-214
Identity = 316/355 (89.01%), Postives = 316/355 (89.01%), Query Frame = 0

Query: 1   MEGDITAIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERK 60
           MEGDITAIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERK
Sbjct: 1   MEGDITAIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERK 60

Query: 61  TRPKITFPVLVQIFICSLSG---------------------------------------Q 120
           TRPKITFPVLVQIFICSLSG                                       Q
Sbjct: 61  TRPKITFPVLVQIFICSLSGATANQIFFFVGLKCTNPTVSSAMANVLPAATFILAVVFRQ 120

Query: 121 ESVRIKTKPGLAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQ 180
           ESVRIKTKPGLAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQ
Sbjct: 121 ESVRIKTKPGLAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQ 180

Query: 181 ENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNV 240
           ENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNV
Sbjct: 181 ENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNV 240

Query: 241 ASWSLKSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALL 300
           ASWSLKSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALL
Sbjct: 241 ASWSLKSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALL 300

Query: 301 HEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEEEDKVEKATNGSQKDDVELQMK 316
           HEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEEEDKVEKATNGSQKDDVELQMK
Sbjct: 301 HEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEEEDKVEKATNGSQKDDVELQMK 355

BLAST of Cp4.1LG11g08570 vs. NCBI nr
Match: KAG7030108.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 589 bits (1519), Expect = 6.56e-211
Identity = 308/354 (87.01%), Postives = 314/354 (88.70%), Query Frame = 0

Query: 1   MEGDITAIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERK 60
           MEGDIT IMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERK
Sbjct: 1   MEGDITTIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERK 60

Query: 61  TRPKITFPVLVQIFICSLSG---------------------------------------Q 120
           TRPKITFPVLVQIFICSLSG                                       Q
Sbjct: 61  TRPKITFPVLVQIFICSLSGATANQIFFFVGLKCTNPTVSSAMANVLPAATFILAVVFRQ 120

Query: 121 ESVRIKTKPGLAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQ 180
           ESVRIKTKPGLAKVMGTIVCICGAMLLSFYHGHTIGLGESK+HWPYVERIMSKTNPTNRQ
Sbjct: 121 ESVRIKTKPGLAKVMGTIVCICGAMLLSFYHGHTIGLGESKLHWPYVERIMSKTNPTNRQ 180

Query: 181 ENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNV 240
           ENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNV
Sbjct: 181 ENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNV 240

Query: 241 ASWSLKSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALL 300
           A+WSLKSPIRL+SALYTG+VCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALL
Sbjct: 241 AAWSLKSPIRLVSALYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALL 300

Query: 301 HEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEEEDKVEKATNGSQKDDVELQM 315
           HEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEEE+KVEKATNGSQKDDVELQ+
Sbjct: 301 HEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEEEEKVEKATNGSQKDDVELQI 354

BLAST of Cp4.1LG11g08570 vs. NCBI nr
Match: XP_022946709.1 (WAT1-related protein At1g09380 [Cucurbita moschata])

HSP 1 Score: 587 bits (1513), Expect = 5.38e-210
Identity = 308/354 (87.01%), Postives = 313/354 (88.42%), Query Frame = 0

Query: 1   MEGDITAIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERK 60
           MEGDIT IMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERK
Sbjct: 1   MEGDITTIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERK 60

Query: 61  TRPKITFPVLVQIFICSLSG---------------------------------------Q 120
           TRPKITFPVLVQIFICSLSG                                       Q
Sbjct: 61  TRPKITFPVLVQIFICSLSGATANQIFFFVGLKCTNPTVSSAMANVLPAATFILAVVFRQ 120

Query: 121 ESVRIKTKPGLAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQ 180
           ESVRIKTKPGLAKVMGTIVC+CGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQ
Sbjct: 121 ESVRIKTKPGLAKVMGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQ 180

Query: 181 ENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNV 240
           ENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNV
Sbjct: 181 ENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNV 240

Query: 241 ASWSLKSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALL 300
           A+WSLKS IRLISALYTG+VCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALL
Sbjct: 241 AAWSLKSSIRLISALYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALL 300

Query: 301 HEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEEEDKVEKATNGSQKDDVELQM 315
           HEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEEE+KVEKATNGSQKDDVELQ+
Sbjct: 301 HEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEEEEKVEKATNGSQKDDVELQI 354

BLAST of Cp4.1LG11g08570 vs. NCBI nr
Match: XP_022999562.1 (WAT1-related protein At1g09380-like [Cucurbita maxima])

HSP 1 Score: 585 bits (1507), Expect = 7.65e-209
Identity = 307/354 (86.72%), Postives = 311/354 (87.85%), Query Frame = 0

Query: 1   MEGDITAIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERK 60
           MEGDITAIMGMVILQ CYAGLSITSKLAMQSGM+PLVLLTYRQFFGTLAIAPFAFFTERK
Sbjct: 16  MEGDITAIMGMVILQFCYAGLSITSKLAMQSGMSPLVLLTYRQFFGTLAIAPFAFFTERK 75

Query: 61  TRPKITFPVLVQIFICSLSG---------------------------------------Q 120
           TRPKITFPVLVQIFICSLSG                                       Q
Sbjct: 76  TRPKITFPVLVQIFICSLSGATANQIFFFVGLKCTNPTVSSAMANVLPAATFILAVVFRQ 135

Query: 121 ESVRIKTKPGLAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQ 180
           ESVRIKTKPG AKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQ
Sbjct: 136 ESVRIKTKPGFAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQ 195

Query: 181 ENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNV 240
           ENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNV
Sbjct: 196 ENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNV 255

Query: 241 ASWSLKSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALL 300
           A+WSL SPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSW LL
Sbjct: 256 AAWSLNSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWTLL 315

Query: 301 HEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEEEDKVEKATNGSQKDDVELQM 315
           HEQLY+GTVLGSILIIIGLYGVLWGKSKEMKVEEEDKVEKATNGSQKDDVELQ+
Sbjct: 316 HEQLYIGTVLGSILIIIGLYGVLWGKSKEMKVEEEDKVEKATNGSQKDDVELQI 369

BLAST of Cp4.1LG11g08570 vs. ExPASy TrEMBL
Match: A0A6J1G4M7 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111450701 PE=3 SV=1)

HSP 1 Score: 587 bits (1513), Expect = 2.60e-210
Identity = 308/354 (87.01%), Postives = 313/354 (88.42%), Query Frame = 0

Query: 1   MEGDITAIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERK 60
           MEGDIT IMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERK
Sbjct: 1   MEGDITTIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERK 60

Query: 61  TRPKITFPVLVQIFICSLSG---------------------------------------Q 120
           TRPKITFPVLVQIFICSLSG                                       Q
Sbjct: 61  TRPKITFPVLVQIFICSLSGATANQIFFFVGLKCTNPTVSSAMANVLPAATFILAVVFRQ 120

Query: 121 ESVRIKTKPGLAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQ 180
           ESVRIKTKPGLAKVMGTIVC+CGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQ
Sbjct: 121 ESVRIKTKPGLAKVMGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQ 180

Query: 181 ENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNV 240
           ENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNV
Sbjct: 181 ENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNV 240

Query: 241 ASWSLKSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALL 300
           A+WSLKS IRLISALYTG+VCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALL
Sbjct: 241 AAWSLKSSIRLISALYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALL 300

Query: 301 HEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEEEDKVEKATNGSQKDDVELQM 315
           HEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEEE+KVEKATNGSQKDDVELQ+
Sbjct: 301 HEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEEEEKVEKATNGSQKDDVELQI 354

BLAST of Cp4.1LG11g08570 vs. ExPASy TrEMBL
Match: A0A6J1KB70 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111493889 PE=3 SV=1)

HSP 1 Score: 585 bits (1507), Expect = 3.71e-209
Identity = 307/354 (86.72%), Postives = 311/354 (87.85%), Query Frame = 0

Query: 1   MEGDITAIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERK 60
           MEGDITAIMGMVILQ CYAGLSITSKLAMQSGM+PLVLLTYRQFFGTLAIAPFAFFTERK
Sbjct: 16  MEGDITAIMGMVILQFCYAGLSITSKLAMQSGMSPLVLLTYRQFFGTLAIAPFAFFTERK 75

Query: 61  TRPKITFPVLVQIFICSLSG---------------------------------------Q 120
           TRPKITFPVLVQIFICSLSG                                       Q
Sbjct: 76  TRPKITFPVLVQIFICSLSGATANQIFFFVGLKCTNPTVSSAMANVLPAATFILAVVFRQ 135

Query: 121 ESVRIKTKPGLAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQ 180
           ESVRIKTKPG AKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQ
Sbjct: 136 ESVRIKTKPGFAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQ 195

Query: 181 ENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNV 240
           ENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNV
Sbjct: 196 ENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNV 255

Query: 241 ASWSLKSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALL 300
           A+WSL SPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSW LL
Sbjct: 256 AAWSLNSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWTLL 315

Query: 301 HEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEEEDKVEKATNGSQKDDVELQM 315
           HEQLY+GTVLGSILIIIGLYGVLWGKSKEMKVEEEDKVEKATNGSQKDDVELQ+
Sbjct: 316 HEQLYIGTVLGSILIIIGLYGVLWGKSKEMKVEEEDKVEKATNGSQKDDVELQI 369

BLAST of Cp4.1LG11g08570 vs. ExPASy TrEMBL
Match: A0A5D3BDZ3 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G00720 PE=3 SV=1)

HSP 1 Score: 501 bits (1289), Expect = 1.18e-176
Identity = 259/325 (79.69%), Postives = 288/325 (88.62%), Query Frame = 0

Query: 1   MEGD-ITAIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTER 60
           MEGD ITA++GM+ILQ+CYAG++I SKLAMQSGMNPLVLLTYRQ FGTLAIAPFAFFTER
Sbjct: 1   MEGDDITALIGMIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFFTER 60

Query: 61  KTRPKITFPVLVQIFICSLSGQESVRIKTKPGLAKVMGTIVCICGAMLLSFYHGHTIGLG 120
           KTRPKITF VL QIF+CSLSGQESVRIKTK G AKV+GTIVC+ GAMLLSFYHGH I LG
Sbjct: 61  KTRPKITFTVLFQIFVCSLSGQESVRIKTKSGFAKVIGTIVCVSGAMLLSFYHGHIINLG 120

Query: 121 ESKIHWPYVERIMSKTNPTNRQENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTST 180
           ESKIHW YVER++ +T PTN Q    LGS+LLL SSF+WALWFVIQARLSVKFKAPYTST
Sbjct: 121 ESKIHWSYVERLIKETTPTNGQGKHVLGSILLLLSSFAWALWFVIQARLSVKFKAPYTST 180

Query: 181 TLLCFMAFFQCGLIAVISEHNVASWSLKSPIRLISALYTGIVCSGLTFSITSWIIQRKGP 240
            LLCFMAFFQCGLIAVISEHN+A+WSLKS IRLI+ALY G+VCS LTFSITSW IQRKGP
Sbjct: 181 ALLCFMAFFQCGLIAVISEHNIAAWSLKSTIRLIAALYAGVVCSALTFSITSWAIQRKGP 240

Query: 241 LYVSIFTPLLLIIVAIFSWALLHEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEE-EDK 300
           LYVSIF+PLLLIIVAI SWALLH+QL+VGTV+GS+LIIIGLY VLWGKSKEMKVE+ +  
Sbjct: 241 LYVSIFSPLLLIIVAILSWALLHQQLHVGTVIGSVLIIIGLYAVLWGKSKEMKVEDHQHN 300

Query: 301 VEKAT----NGS----QKDDVELQM 315
           +EKAT    NGS    +KDD+ELQ+
Sbjct: 301 MEKATIEKHNGSHIVEEKDDLELQI 325

BLAST of Cp4.1LG11g08570 vs. ExPASy TrEMBL
Match: A0A5A7TYK5 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G001930 PE=3 SV=1)

HSP 1 Score: 481 bits (1239), Expect = 1.94e-168
Identity = 259/364 (71.15%), Postives = 288/364 (79.12%), Query Frame = 0

Query: 1   MEGD-ITAIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTER 60
           MEGD ITA++GM+ILQ+CYAG++I SKLAMQSGMNPLVLLTYRQ FGTLAIAPFAFFTER
Sbjct: 1   MEGDDITALIGMIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFFTER 60

Query: 61  KTRPKITFPVLVQIFICSLSG--------------------------------------- 120
           KTRPKITF VL QIF+CSLSG                                       
Sbjct: 61  KTRPKITFTVLFQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFR 120

Query: 121 QESVRIKTKPGLAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNR 180
           QESVRIKTK G AKV+GTIVC+ GAMLLSFYHGH I LGESKIHW YVER++ +T PTN 
Sbjct: 121 QESVRIKTKSGFAKVIGTIVCVSGAMLLSFYHGHIINLGESKIHWSYVERLIKETTPTNG 180

Query: 181 QENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHN 240
           Q    LGS+LLL SSF+WALWFVIQARLSVKFKAPYTST LLCFMAFFQCGLIAVISEHN
Sbjct: 181 QGKHVLGSILLLLSSFAWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHN 240

Query: 241 VASWSLKSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWAL 300
           +A+WSLKS IRLI+ALY G+VCS LTFSITSW IQRKGPLYVSIF+PLLLIIVAI SWAL
Sbjct: 241 IAAWSLKSTIRLIAALYAGVVCSALTFSITSWAIQRKGPLYVSIFSPLLLIIVAILSWAL 300

Query: 301 LHEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEE-EDKVEKAT----NGS----QKDDV 315
           LH+QL+VGTV+GS+LIIIGLY VLWGKSKEMKVE+ +  +EKAT    NGS    +KDD+
Sbjct: 301 LHQQLHVGTVIGSVLIIIGLYAVLWGKSKEMKVEDHQHNMEKATIEKHNGSHIVEEKDDL 360

BLAST of Cp4.1LG11g08570 vs. ExPASy TrEMBL
Match: A0A1S3BWI1 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103494009 PE=3 SV=1)

HSP 1 Score: 481 bits (1239), Expect = 1.94e-168
Identity = 259/364 (71.15%), Postives = 288/364 (79.12%), Query Frame = 0

Query: 1   MEGD-ITAIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTER 60
           MEGD ITA++GM+ILQ+CYAG++I SKLAMQSGMNPLVLLTYRQ FGTLAIAPFAFFTER
Sbjct: 1   MEGDDITALIGMIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFFTER 60

Query: 61  KTRPKITFPVLVQIFICSLSG--------------------------------------- 120
           KTRPKITF VL QIF+CSLSG                                       
Sbjct: 61  KTRPKITFTVLFQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFR 120

Query: 121 QESVRIKTKPGLAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNR 180
           QESVRIKTK G AKV+GTIVC+ GAMLLSFYHGH I LGESKIHW YVER++ +T PTN 
Sbjct: 121 QESVRIKTKSGFAKVIGTIVCVSGAMLLSFYHGHIINLGESKIHWSYVERLIKETTPTNG 180

Query: 181 QENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHN 240
           Q    LGS+LLL SSF+WALWFVIQARLSVKFKAPYTST LLCFMAFFQCGLIAVISEHN
Sbjct: 181 QGKHVLGSILLLLSSFAWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHN 240

Query: 241 VASWSLKSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWAL 300
           +A+WSLKS IRLI+ALY G+VCS LTFSITSW IQRKGPLYVSIF+PLLLIIVAI SWAL
Sbjct: 241 IAAWSLKSTIRLIAALYAGVVCSALTFSITSWAIQRKGPLYVSIFSPLLLIIVAILSWAL 300

Query: 301 LHEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEE-EDKVEKAT----NGS----QKDDV 315
           LH+QL+VGTV+GS+LIIIGLY VLWGKSKEMKVE+ +  +EKAT    NGS    +KDD+
Sbjct: 301 LHQQLHVGTVIGSVLIIIGLYAVLWGKSKEMKVEDHQHNMEKATIEKHNGSHIVEEKDDL 360

BLAST of Cp4.1LG11g08570 vs. TAIR 10
Match: AT1G09380.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 349.4 bits (895), Expect = 3.1e-96
Identity = 178/358 (49.72%), Postives = 239/358 (66.76%), Query Frame = 0

Query: 2   EGDITAIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERKT 61
           + D+   + MV++Q+ YAG++ITSK+AM++GM PL+L+ YRQ F T+A  P AFF ERKT
Sbjct: 3   KSDMLPFLAMVLVQIGYAGMNITSKMAMEAGMKPLILVAYRQIFATIATFPVAFFLERKT 62

Query: 62  RPKITFPVLVQIFICSLSG---------------------------------------QE 121
           RPKIT  +LVQ+F CS++G                                       QE
Sbjct: 63  RPKITLRILVQVFFCSITGATGNQVLYFVGLQNSSPTIACALTNLLPAVTFLLAAIFRQE 122

Query: 122 SVRIKTKPGLAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQE 181
           +V IK   G AKV+GT+VC+ GAM+LSFYHGHTIG+GESKIHW Y E I +K   ++   
Sbjct: 123 TVGIKKASGQAKVIGTLVCVIGAMVLSFYHGHTIGIGESKIHWAYAENI-TKHGSSSGHS 182

Query: 182 NRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNVA 241
           N  LG  L++ ++ SWA WF+IQ ++S  F APYTST L+C M   QCG IA+IS+H ++
Sbjct: 183 NFFLGPFLIMAAAVSWAAWFIIQTKMSETFAAPYTSTLLMCLMGSIQCGAIALISDHTIS 242

Query: 242 SWSLKSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALLH 301
            WSL SP+R ISALY G+V S L F + SW +QRKGPLYVS+F+PLLL++VAIFSWALL 
Sbjct: 243 DWSLSSPLRFISALYAGVVASALAFCLMSWAMQRKGPLYVSVFSPLLLVVVAIFSWALLE 302

Query: 302 EQLYVGTVLGSILIIIGLYGVLWGKSKEM--KVEEEDKVEKATN---GSQKDDVELQM 316
           E+LY GT +GS L++IGLYGVLWGK +E+  K EE +KV++  +       +D+E ++
Sbjct: 303 EKLYTGTFMGSALVVIGLYGVLWGKDREVSEKEEEREKVKQQNHKVKSESNEDIESRL 359

BLAST of Cp4.1LG11g08570 vs. TAIR 10
Match: AT3G30340.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 202.2 bits (513), Expect = 6.1e-52
Identity = 111/324 (34.26%), Postives = 187/324 (57.72%), Query Frame = 0

Query: 7   AIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERKTRPKIT 66
           A++ M ++ +  + +++  K  +  G+N +V  TYR   GTL + PFA F ER  RPK+T
Sbjct: 11  AVLMMSMINIGLSVVNVMFKKMIDEGLNRMVATTYRLAVGTLFLIPFAIFLERHNRPKLT 70

Query: 67  FPVLVQIFICSLSG---------------------------------------QESVRIK 126
             +L  +F  +L G                                       QE++ IK
Sbjct: 71  GRILCSLFFSALLGTSLVQYFFLIGLEYTSSTFSLAFSNMVPSVTFALALVFRQETLNIK 130

Query: 127 TKPGLAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQENR-TL 186
           +  G AK++GT++CICGA++L+ Y G  +    S+ H  ++E   ++T+ T     +  +
Sbjct: 131 SNVGRAKLLGTMICICGALVLTLYKGTAL----SREHSTHME-THTRTDSTGAMTQKWAM 190

Query: 187 GSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNVASWSL 246
           GS++L+ S   W+ WF++QA++S  +   YTSTT+L F    Q  L+++ISE + + W +
Sbjct: 191 GSIMLVISIIIWSSWFIVQAKISRVYPCQYTSTTILSFFGVIQSALLSLISERSTSMWVV 250

Query: 247 KSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALLHEQLY 291
           K   ++++ LY+GIV SGL +   SW ++++G ++ S F PL+ +  AIFS++ LHEQ+Y
Sbjct: 251 KDKFQVLALLYSGIVGSGLCYVGMSWCLRQRGAVFTSSFIPLIQVFAAIFSFSFLHEQIY 310

BLAST of Cp4.1LG11g08570 vs. TAIR 10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 188.7 bits (478), Expect = 7.0e-48
Identity = 112/344 (32.56%), Postives = 189/344 (54.94%), Query Frame = 0

Query: 11  MVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERKTRPKITFPVL 70
           M+ LQ  YAG++I +K+++ +GM+  VL+ YR    T  IAPFAFF ERK +PKITF + 
Sbjct: 22  MISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQPKITFSIF 81

Query: 71  VQIFICSLSG---------------------------------------QESVRIKTKPG 130
           +Q+FI  L G                                        E + +K    
Sbjct: 82  MQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDLKKLWC 141

Query: 131 LAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQENRTL--GSV 190
            AK+ GT+V + GAML++ Y G  + L  +K          + T+  N   ++    GS+
Sbjct: 142 QAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSSSDKEFLKGSI 201

Query: 191 LLLCSSFSWALWFVIQAR-LSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNVASWSLKS 250
           LL+ ++ +WA  FV+QA+ L    K   + TTL+CF+   Q   +  + EHN ++W +  
Sbjct: 202 LLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHNPSAWRIGW 261

Query: 251 PIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALLHEQLYVG 310
            + L++A Y+GIV S +++ +   +++++GP++ + F+PL+++IVA+    +L E++++G
Sbjct: 262 DMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFVLAEKIFLG 321

Query: 311 TVLGSILIIIGLYGVLWGKSKEMKVEEEDKVEKATNGSQKDDVE 313
            V+G++LI+IGLY VLWGK KE +V   +  +  +N    +DVE
Sbjct: 322 GVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSKVTEDVE 365

BLAST of Cp4.1LG11g08570 vs. TAIR 10
Match: AT1G68170.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 186.4 bits (472), Expect = 3.5e-47
Identity = 118/345 (34.20%), Postives = 184/345 (53.33%), Query Frame = 0

Query: 4   DITAIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERKTRP 63
           DITA   MV++Q+  AGL+I  KLAM+ GMNP VL+ YR  F TL + P  F  +RK RP
Sbjct: 3   DITA---MVVVQIATAGLNIFFKLAMEDGMNPSVLVAYRLLFATLFMIPICFIFQRKKRP 62

Query: 64  KITFPVLVQIFICSLSG---------------------------------------QESV 123
           + T  +++   +  L G                                        ESV
Sbjct: 63  EFTCRLMLLALLSGLLGVVIPSILTITGLALTSATFTSAAGVLTPLVTFIFAALLRMESV 122

Query: 124 RIKTKPGLAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNRQENR 183
           R+ +  GLAKV GT+  + GA++  FY G  I L  + ++     R  S+   T+     
Sbjct: 123 RLGSSVGLAKVFGTLFGVGGALVFIFYRGIEIRLWSTHVNLVNQPRDSSRDATTHHIS-- 182

Query: 184 TLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNVASW 243
            LG++L+   + S +LWF++Q ++S +F  PY + TL+  M      L+A+  EH++  W
Sbjct: 183 ILGALLVFGGNISISLWFLLQVKISKQFGGPYWNATLMNMMGGVVAMLVALCWEHDLDEW 242

Query: 244 SLKSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALLHEQ 303
            L   IRL++  Y  I+ SG+  ++ +W I+ +GPL+VS+F+P+ L+IVA+    LL E 
Sbjct: 243 RLGWNIRLLTIAYAAILISGMVVAVNAWCIESRGPLFVSVFSPVGLVIVALVGSFLLDET 302

Query: 304 LYVGTVLGSILIIIGLYGVLWGKSKEMKVEEEDKVEKATNGSQKD 310
           L++G+++G+++I+  LY VLW K+KEMK          TN + KD
Sbjct: 303 LHLGSIIGTVIIVGALYIVLWAKNKEMKSMLTTSDHNETNKTSKD 342

BLAST of Cp4.1LG11g08570 vs. TAIR 10
Match: AT5G13670.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 184.9 bits (468), Expect = 1.0e-46
Identity = 112/327 (34.25%), Postives = 180/327 (55.05%), Query Frame = 0

Query: 9   MGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTERKTRPKITFP 68
           + +V +Q  YA +SI +KLA+  GM+P VL+ YR    +  I PFA   ER TRPK+TF 
Sbjct: 10  IAIVFIQCLYALMSIVAKLALNKGMSPHVLVAYRMAVASALITPFALILERNTRPKLTFK 69

Query: 69  VLVQIFICSL-----------SGQ----------------------------ESVRIKTK 128
           +L+QI I SL           SG                             E V I+ +
Sbjct: 70  ILLQIAILSLFEPVVEQNLYYSGMKLTTATFTSALCNALPAMTFIMACVFKLEKVTIERR 129

Query: 129 PGLAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTN-----RQENR 188
              AK++GT+V I GAML++F  G+ I L       P+          T+     +Q + 
Sbjct: 130 HSQAKLVGTMVAIGGAMLMTFVKGNVIEL-------PWTSNSRGLNGHTHAMRIPKQADI 189

Query: 189 TLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEH-NVAS 248
             GS++L+ S FSW+ + ++QA++  ++KA  + T L+C M   +  ++ +I E  N++ 
Sbjct: 190 ARGSIMLVASCFSWSCYIILQAKILAQYKAELSLTALMCIMGMLEATVMGLIWERKNMSV 249

Query: 249 WSLKSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWALLHE 291
           W +   + L++++Y G+V SGL + +  W  + +GP++VS F PL +++VAI S  +  E
Sbjct: 250 WKINPDVTLLASIYGGLV-SGLAYYVIGWASKERGPVFVSAFNPLSMVLVAILSTFVFLE 309

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GXB44.4e-9549.72WAT1-related protein At1g09380 OS=Arabidopsis thaliana OX=3702 GN=At1g09380 PE=2... [more]
Q9LI658.6e-5134.26WAT1-related protein At3g30340 OS=Arabidopsis thaliana OX=3702 GN=At3g30340 PE=2... [more]
Q9FL419.9e-4732.56WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2... [more]
F4HVM34.9e-4634.20WAT1-related protein At1g68170 OS=Arabidopsis thaliana OX=3702 GN=At1g68170 PE=3... [more]
Q9FNA51.4e-4534.25WAT1-related protein At5g13670 OS=Arabidopsis thaliana OX=3702 GN=At5g13670 PE=2... [more]
Match NameE-valueIdentityDescription
XP_023545290.11.04e-222100.00WAT1-related protein At1g09380 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023545289.11.84e-21489.01WAT1-related protein At1g09380 isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG7030108.16.56e-21187.01WAT1-related protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022946709.15.38e-21087.01WAT1-related protein At1g09380 [Cucurbita moschata][more]
XP_022999562.17.65e-20986.72WAT1-related protein At1g09380-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1G4M72.60e-21087.01WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111450701 PE=3 SV=1[more]
A0A6J1KB703.71e-20986.72WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111493889 PE=3 SV=1[more]
A0A5D3BDZ31.18e-17679.69WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546... [more]
A0A5A7TYK51.94e-16871.15WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385... [more]
A0A1S3BWI11.94e-16871.15WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103494009 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G09380.13.1e-9649.72nodulin MtN21 /EamA-like transporter family protein [more]
AT3G30340.16.1e-5234.26nodulin MtN21 /EamA-like transporter family protein [more]
AT5G07050.17.0e-4832.56nodulin MtN21 /EamA-like transporter family protein [more]
AT1G68170.13.5e-4734.20nodulin MtN21 /EamA-like transporter family protein [more]
AT5G13670.11.0e-4634.25nodulin MtN21 /EamA-like transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 146..284
e-value: 1.2E-18
score: 67.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 296..316
NoneNo IPR availablePANTHERPTHR31218:SF344WAT1-RELATED PROTEINcoord: 7..80
NoneNo IPR availablePANTHERPTHR31218:SF344WAT1-RELATED PROTEINcoord: 81..311
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 208..289
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 7..80
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 81..311

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG11g08570.1Cp4.1LG11g08570.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity