Cp4.1LG11g08010 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG11g08010 vs. ExPASy Swiss-Prot
Match: Q304B9 (Neutral ceramidase 2 OS=Arabidopsis thaliana OX=3702 GN=NCER2 PE=3 SV=1)

HSP 1 Score: 742.7 bits (1916), Expect = 3.9e-213
Identity = 383/662 (57.85%), Postives = 477/662 (72.05%), Query Frame = 0

Query: 47  FGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESL 106
           +G LY E+N+AISGIHTHAGPGGYLQY+ Y +TSLGFV+QSFD +VN IEQSIVQAHESL
Sbjct: 102 YGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFDVVVNGIEQSIVQAHESL 161

Query: 107 KPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAF 166
           +PG   +NKG++ +AG+NRSPS+YL NP  ER++Y  +VD +M+LVK V+   G + G+F
Sbjct: 162 RPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEMTLVKFVDSQLGPT-GSF 221

Query: 167 SWFPTHGTSMSRDNKLISGDNKGAAARFFEDWV------AAASNRSTRATSSNKSDIV-- 226
           +WF THGTSMSR N LISGDNKGAAARF EDW       + +S    R  S+  SD    
Sbjct: 222 NWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENGQKNSVSSRNIPRRVSTIVSDFSRN 281

Query: 227 --ELMKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTD 286
              L+  A T K++ G    K+    ++VR      FV AFCQSN GDV+PN LG FC D
Sbjct: 282 RDRLLDIAATYKSSRGHSVDKSLDVKTRVRNGSKRKFVSAFCQSNCGDVSPNTLGTFCID 341

Query: 287 SGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEID 346
           +G PCDFN S+C+G + LC GRGPG+PDE  ST+IIGE+QF  AV+LF  ATE+L G+I 
Sbjct: 342 TGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIG 401

Query: 347 FRHVYLNFTDIEVEL----GGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLW 406
           ++H YL+F++++V +    GG   VKTCPAA+G GFAAGTTDGPG F F+QGD Q N  W
Sbjct: 402 YQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFW 461

Query: 407 KKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEF 466
           + +R+ LR P   QV CQKPK +LLDTGEM EPY WAP+ILPIQILR+G+L+ILSVPGEF
Sbjct: 462 RLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEF 521

Query: 467 TTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTL 526
           TTMAGRRLR+A+K  LIS+   EF ++ HVVIAGLTN YSQY+AT EEYE QRYE ASTL
Sbjct: 522 TTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTL 581

Query: 527 YGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGD 586
           YG HTL+AYIQEFKKLA A+  G  + +    PPDL   Q+  +S   ++S P GV FGD
Sbjct: 582 YGRHTLTAYIQEFKKLATALVNGLTLPRG-PQPPDLLDKQISLLSPVVVDSTPLGVKFGD 641

Query: 587 IQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERL-EKRRWTPVYDDDD 646
           ++ D+  P    F+RG  Q+  A FWS  PR DL+TEG++AVVE L E  +W PVYDDDD
Sbjct: 642 VKADV--PPKSTFRRG--QQVNATFWSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDD 701

Query: 647 FSLVFKWALDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAF 694
           FSL FKW+      ++S ATIEW +P  A  GVYR+RH+G+SKS   S +  F+G+SSAF
Sbjct: 702 FSLKFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRHYGASKSLFGSIS-SFSGSSSAF 756

BLAST of Cp4.1LG11g08010 vs. ExPASy Swiss-Prot
Match: Q0JL46 (Neutral ceramidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0624000 PE=1 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 2.5e-212
Identity = 394/775 (50.84%), Postives = 514/775 (66.32%), Query Frame = 0

Query: 3   RQLLKVVATAVLAAVSCCCADGDWLVGVGSFDMTGPAAEVNMMG---------------- 62
           R  L ++   VL   S   +   +LVG+GSFD+TGPAA+VNMMG                
Sbjct: 18  RVWLWLLLALVLLNCSLVLSASPYLVGMGSFDITGPAADVNMMGYANTEQIASGIHFRLK 77

Query: 63  ---------------------------------------FGNLYREENIAISGIHTHAGP 122
                                                  +G+LY E N+AISGIHTHAGP
Sbjct: 78  SRAFIVAEPNGKRVVFVNIDACMASQIVTIKVLERLKARYGDLYNENNVAISGIHTHAGP 137

Query: 123 GGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESLKPGFMLINKGEVENAGINRSP 182
           GGYLQY+VY +TSLGFV+QSFD IV+ IEQSIV+AH +L+PG + +NKG++ +AG+NRSP
Sbjct: 138 GGYLQYVVYIVTSLGFVRQSFDVIVDGIEQSIVEAHNNLRPGKIFVNKGDLLDAGVNRSP 197

Query: 183 SAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDN 242
           SAYL NP EER++Y  NVD +M+L+K V+   G  +G+F+WF THGTSMSR N LISGDN
Sbjct: 198 SAYLNNPAEERSKYEYNVDKEMTLIKFVDDELG-PVGSFNWFATHGTSMSRTNSLISGDN 257

Query: 243 KGAAARFFEDWV-------AAASNRSTRATSSNKSDIV----------------ELMKKA 302
           KGAAARF EDW         +A   S    S +K+ ++                +L++ A
Sbjct: 258 KGAAARFMEDWAEQMGLPKQSAHANSDDLRSLHKTSVLPRRVSTIIPEPNEITDDLIQLA 317

Query: 303 ETIKATGGKRCSKTS-SQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDF 362
            + +A+GG+R + +S ++  +  + +   FV AFCQSN GDV+PNVLG FC D+  PCDF
Sbjct: 318 SSYEASGGRRLAGSSITRRIRSTQQNKPKFVSAFCQSNCGDVSPNVLGTFCIDTNLPCDF 377

Query: 363 NRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLN 422
           N S+C+G + LC GRGPG+PDE  ST++IG RQFLKA DLF +A+E + G+ID+RH YL+
Sbjct: 378 NHSTCNGKNELCYGRGPGYPDEFESTRVIGNRQFLKARDLFDSASEEIQGKIDYRHTYLD 437

Query: 423 FTDIEVEL----GGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSL 482
           F+ +EV++    GG   VKTCPAA+G  FAAGTTDGPG F F+QGD + N  WK +R+ L
Sbjct: 438 FSKLEVKVSTSAGGQQTVKTCPAAMGFAFAAGTTDGPGAFDFRQGDVKGNPFWKLVRNLL 497

Query: 483 RKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRR 542
           + P + QV C  PK +LLDTGEM EPY WAPAILP+Q++R+G+L+IL VPGEFTTMAGRR
Sbjct: 498 KTPGKDQVECHSPKPILLDTGEMKEPYDWAPAILPVQMIRIGQLVILCVPGEFTTMAGRR 557

Query: 543 LREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTLS 602
           LR+AVK T++++GN EFD + HVV+AGLTN+YSQY+ T EEY+ QRYE ASTLYGPHTLS
Sbjct: 558 LRDAVK-TVLTSGNSEFDKNIHVVLAGLTNSYSQYITTFEEYQIQRYEGASTLYGPHTLS 617

Query: 603 AYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKL 662
           AYIQEF+KLA+A+   +++      PPD+   Q+  +     +S P GV FGD+  D+  
Sbjct: 618 AYIQEFQKLAMAMIANKEVPTNF-QPPDMLDKQIGLLPGVVFDSTPLGVKFGDVNSDV-- 677

Query: 663 PKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLE-KRRWTPVYDDDDFSLVFKW 694
           P    F +GS    T  F+SA PR DLLT+GT+A+VE+L+    W PVYDDDD+SL FKW
Sbjct: 678 PGNSTFNKGSTVNAT--FYSACPRNDLLTDGTFALVEKLDGNNNWVPVYDDDDWSLRFKW 737

BLAST of Cp4.1LG11g08010 vs. ExPASy Swiss-Prot
Match: F4HQM3 (Neutral ceramidase 1 OS=Arabidopsis thaliana OX=3702 GN=NCER1 PE=2 SV=1)

HSP 1 Score: 718.8 bits (1854), Expect = 6.0e-206
Identity = 383/749 (51.13%), Postives = 499/749 (66.62%), Query Frame = 0

Query: 25  DWLVGVGSFDMTGPAAEVNMMG-------------------------------------- 84
           ++L+G+GS+D+TGPAA+VNMMG                                      
Sbjct: 36  EYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFIVSEPQGKRVVFVNLDAC 95

Query: 85  -----------------FGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFD 144
                            +G+LY E+N+ ISGIHTHAGPGGYLQY+VY +TSLGFV+QSFD
Sbjct: 96  MASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFD 155

Query: 145 AIVNAIEQSIVQAHESLKPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDM 204
           A+V+ IE SI+QAHE+L+PG + +N GE+ +AG+NRSPSAYL NP +ER+++  NVD +M
Sbjct: 156 ALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPSAYLNNPSKERSKHKYNVDKEM 215

Query: 205 SLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAA-------- 264
           +L+K V+   G  +G+F+WF THGTSMSR N LISGDNKGAA+RF EDW           
Sbjct: 216 TLLKFVDDQWG-PVGSFNWFATHGTSMSRTNSLISGDNKGAASRFMEDWYEQNTAERSYS 275

Query: 265 ---ASNRSTRATSS----NKSDIVELMKKAETIKATGGKRCSKTSSQSSKV----RKNDG 324
               S+   R  SS    ++    EL++ A   ++  GK  ++ SS + +V    RK D 
Sbjct: 276 EEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKPVTRISSSARRVRSALRKADK 335

Query: 325 SLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTK 384
             FV AFCQ+N GDV+PNVLGAFC D+G PCDFN S+C G + +C GRGPG+PDE  ST+
Sbjct: 336 PGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGGKNEMCYGRGPGYPDEFESTR 395

Query: 385 IIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVEL----GGDNVVKTCPAALGP 444
           IIGERQF  A++LF  A+E+L G++D+RHVY++F+ + V L    G   VVKTCPAA+G 
Sbjct: 396 IIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKKDGKSEVVKTCPAAMGF 455

Query: 445 GFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPY 504
            FAAGTTDGPG F F QGD + N  W+ +R+ L+ P + Q+ C  PK +LLDTGEM +PY
Sbjct: 456 AFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDCHYPKPILLDTGEMTKPY 515

Query: 505 AWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAG 564
            WAP+IL +Q+LR+G+L ILSVPGEFTTMAGRRLR AVK  L ++GN +   + HVVIAG
Sbjct: 516 DWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKTQLKNSGNKDLSGEIHVVIAG 575

Query: 565 LTNAYSQYVATLEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPP 624
           L N YSQYV T EEY+ QRYE ASTLYGPHTLS YIQEFKKL+ ++     + +P   PP
Sbjct: 576 LANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLSKSLVLDMPV-QPGPQPP 635

Query: 625 DLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDL 684
           DL   QL F++   +++ P+G +FGD+  D+  PK    KRG+ Q  T  F SA PR DL
Sbjct: 636 DLLDKQLSFLTPVMMDTTPSGDSFGDVISDV--PKNLSLKRGNGQ-VTVVFRSACPRNDL 695

Query: 685 LTEGTYAVVERLEK--RRWTPVYDDDDFSLVFKWALDNNTFTKSLATIEWDIPIDANPGV 694
           LTEGT+ +VERLE+  + WTPVYDDDD  L FKW+      ++S AT+EW IP  A+PGV
Sbjct: 696 LTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRSQATVEWRIPESASPGV 755

BLAST of Cp4.1LG11g08010 vs. ExPASy Swiss-Prot
Match: F4KHQ8 (Neutral ceramidase 3 OS=Arabidopsis thaliana OX=3702 GN=NCER3 PE=2 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 2.6e-193
Identity = 360/666 (54.05%), Postives = 459/666 (68.92%), Query Frame = 0

Query: 47  FGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESL 106
           +G LY EEN+AISG HTHAGPGGYLQY++Y +TSLGFV QSF+A+V+ IEQSI+QAHE+L
Sbjct: 105 YGELYTEENVAISGTHTHAGPGGYLQYILYLVTSLGFVHQSFNALVDGIEQSIIQAHENL 164

Query: 107 KPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAF 166
           +PG +LINKGE+ +AG+NRSPSAYL NP  ER++Y  +VD +M+LVK V+          
Sbjct: 165 RPGSILINKGELLDAGVNRSPSAYLNNPAHERSKYEYDVDKEMTLVKFVD---------- 224

Query: 167 SWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAAASN------RSTRATSSNKSDIV-- 226
                              D  G  AR  EDW    +        S R  SS  SD    
Sbjct: 225 -------------------DQWGPVARIMEDWFERENGCRSVDVESPRRVSSIISDPYDQ 284

Query: 227 ELMKKAETIKATGGKRCSKTSSQSSKVRKN----DGSLFVGAFCQSNVGDVTPNVLGAFC 286
           +LM+ A ++ +TGGK  ++ SS + +VR      D   FV AFCQ+N GDV+PNVLGAFC
Sbjct: 285 DLMEMASSLLSTGGKTVTRMSSVARRVRSRFRHADKPRFVSAFCQTNCGDVSPNVLGAFC 344

Query: 287 TDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGE 346
            D+G PC+FN+S+C G +  C GRGPG+PDE  ST+IIGERQF KA DLFT A+E + G+
Sbjct: 345 IDTGLPCEFNQSTCGGKNEQCYGRGPGYPDEFESTRIIGERQFKKAADLFTKASEEIQGK 404

Query: 347 IDFRHVYLNFTDIEVEL----GGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINK 406
           +D+RH Y++F+ +EV +    GG  VVKTCPAA+G GFAAGTTDGPG F F+QGD Q N 
Sbjct: 405 VDYRHAYVDFSQLEVTINGQNGGSEVVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNP 464

Query: 407 LWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPG 466
            W+ +R+ L+ P+E QV CQ+PK +LLDTGEM +PY WAP+ILP+QILR+G+L+IL VPG
Sbjct: 465 FWRLVRNLLKNPTEEQVRCQRPKPILLDTGEMKQPYDWAPSILPVQILRIGQLVILCVPG 524

Query: 467 EFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAAS 526
           EFTTMAGRRLR+AVK  L    NG    +  VVIAGLTN+YSQY+AT EEY+ QRYE AS
Sbjct: 525 EFTTMAGRRLRDAVKTVLKEGSNGR---EFSVVIAGLTNSYSQYIATFEEYQVQRYEGAS 584

Query: 527 TLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNF 586
           TLYGPHTLS YIQEFKKLA  +   +    P   PPDL   Q+  ++    +  P G  F
Sbjct: 585 TLYGPHTLSGYIQEFKKLANDLLSAQ-TTDPGPQPPDLLHKQISLLTPVVADMTPIGTAF 644

Query: 587 GDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVER-LEKRR-WTPVYD 646
           GD+  D+  P++  F++G+      +F SANPR DL+TEGT+A+VER LE R  W PVYD
Sbjct: 645 GDVTSDV--PRLSKFRKGA-DIVRVQFRSANPRNDLMTEGTFALVERWLEGRETWVPVYD 704

Query: 647 DDDFSLVFKWALDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGAS 695
           DDDF L FKW+      T+S ATIEW IP  A+PGVYR+ HFGS+K+ I+S + +F+G+S
Sbjct: 705 DDDFCLRFKWSRPFKLSTQSTATIEWRIPETASPGVYRITHFGSAKTPISSIH-HFSGSS 733

HSP 2 Score: 53.1 bits (126), Expect = 1.4e-05
Identity = 25/49 (51.02%), Postives = 38/49 (77.55%), Query Frame = 0

Query: 14 LAAVSCCCADGDWLVGVGSFDMTGPAAEVNMMGFGNLYREENIAISGIH 63
          L  ++C  +D D+L+G+GS+D+TGPAA+VNMMG+ N+   E +A SG+H
Sbjct: 17 LLRLTCIFSDSDYLMGLGSYDITGPAADVNMMGYANM---EQVA-SGVH 61

BLAST of Cp4.1LG11g08010 vs. ExPASy Swiss-Prot
Match: Q55G11 (Neutral ceramidase B OS=Dictyostelium discoideum OX=44689 GN=dcd2B PE=3 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 4.1e-130
Identity = 283/737 (38.40%), Postives = 398/737 (54.00%), Query Frame = 0

Query: 23  DGDWLVGVGSFDMTGPAAEVNMMGFG---------------------------------- 82
           D  + +G G +D+TG +AEVN+MG+                                   
Sbjct: 43  DSSYQIGAGIYDITGASAEVNLMGYANPLQVGAGIHFRQRARAFVFVDSNGNRAVYVSTD 102

Query: 83  ----------------------NLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQ 142
                                 N+Y E N+ +SG HTH+GP G+ QY +Y ITSLGF ++
Sbjct: 103 SCMIFQEVKIHVVELLQDIFGPNVYTEANVLLSGTHTHSGPAGFSQYALYGITSLGFYKK 162

Query: 143 SFDAIVNAIEQSIVQAHESLKPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVD 202
           +FD I N I Q+IV+AH+S++P  M    GE+ N  INRSP AY  NP EE+A Y +NVD
Sbjct: 163 NFDTICNGIVQAIVKAHKSVQPANMFTETGELWNTNINRSPFAYDNNPEEEKAMYDSNVD 222

Query: 203 TDMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAAASNRS 262
            +M++++ +E  +G    A S+F  H TSM+  N LISGDNKG A+  +E  V       
Sbjct: 223 KNMTVLR-IEDMNGNPFAAISFFAVHCTSMNNTNHLISGDNKGYASYLWEKQVNG----- 282

Query: 263 TRATSSNKSDIVELMKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVT 322
                                  T GK                   FV AF QSN GDV+
Sbjct: 283 ---------------------PGTAGK-----------------GPFVAAFGQSNEGDVS 342

Query: 323 PNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEIL-STKIIGERQFLKAVDLFT 382
           PN  G  C D G PCD+  S+C+G +  C   GPG   ++  ST+IIG  QF KA++LF 
Sbjct: 343 PNTRGPTCRD-GSPCDYKTSTCNGRNEECWSLGPGKDGDMFESTQIIGGNQFNKALELFN 402

Query: 383 TATERLTGEIDFRHVYLNFTDIEVE----LGGDNVVKTCPAALGPGFAAGTTDGPGVFGF 442
            A+ +++G + +RH ++ FT++ VE     G DN   TC  A+G  FAAGTTDGPG F F
Sbjct: 403 NASIQVSGPVQYRHSWVQFTNVSVEPPYNSGVDNAT-TCRGAMGYSFAAGTTDGPGAFNF 462

Query: 443 QQGD--TQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILR 502
            Q D  T  N  W  + D + KP+  Q+ CQ PK +LLD G M EP  W P ++PIQI+ 
Sbjct: 463 VQSDNNTSGNPFWNFIGDFIAKPTPDQIRCQSPKPILLDVG-MVEPIPWVPDVMPIQIVT 522

Query: 503 LGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLE 562
           +G++++++VPGEFTTM+GRRLR +V+E +     GE  ++  V+IAGL+N YS Y+AT E
Sbjct: 523 IGQIVLVAVPGEFTTMSGRRLRNSVREII-----GESIENPIVLIAGLSNTYSGYIATFE 582

Query: 563 EYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKI-AKPIVSPPDLSSVQLRFVSD 622
           EY+ QRYE AST++GPHTL +Y+QEF KLA +I  G  + A P  +P +L+   L F+  
Sbjct: 583 EYQVQRYEGASTVFGPHTLGSYMQEFGKLAQSIVDGTTVPAGP--TPRNLTGHTLFFLPP 642

Query: 623 PFIESPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERL 682
             +++ P+  +FG++  D+ L           +  +  F+  NPR D + E ++  V+ L
Sbjct: 643 VIVDAAPDFDDFGEVSIDVNL------NYSVNETVSCVFYGGNPRNDFMIESSFLSVDLL 702

Query: 683 E-KRRWTPVYDDDDFSLVFKWALDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKSAI 694
               +WT V DD D+   FKW + +  F  SL TIEW I  D  PG YR+ H G +K   
Sbjct: 703 TGTDQWTTVLDDGDWDTKFKWKMHDLGF--SLITIEWVIAPDTTPGTYRITHSGFAKKNP 717

BLAST of Cp4.1LG11g08010 vs. NCBI nr
Match: KAG7030054.1 (Neutral ceramidase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1339 bits (3466), Expect = 0.0
Identity = 686/766 (89.56%), Postives = 689/766 (89.95%), Query Frame = 0

Query: 1   MRRQLLKVVATAVLAAVSCCCA-DGDWLVGVGSFDMTGPAAEVNMMG------------- 60
           MRRQLLKVVATAVLAAVSCCCA DGDWLVGVGSFDMTGPAAEVNMMG             
Sbjct: 1   MRRQLLKVVATAVLAAVSCCCAADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHF 60

Query: 61  ----------------------------------------------------------FG 120
                                                                     FG
Sbjct: 61  RLRARTFIVAKSVDGPRIAFVNLDAGMASQLVTIKLLERLKASLTFLSKLKPRHFIQRFG 120

Query: 121 NLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESLKP 180
           NLY EEN+AISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAH SLKP
Sbjct: 121 NLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKP 180

Query: 181 GFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAFSW 240
           GF+LINKGEVENAGINRSPSAYLMNPPEERARYPTNVDT+MSLVKVVEGGSGVSLGAFSW
Sbjct: 181 GFILINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSW 240

Query: 241 FPTHGTSMSRDNKLISGDNKGAAARFFEDWVAAASNRSTRATSSNKSDIVELMKKAETIK 300
           FPTHGTSMSRDNKLISGDNKGAAARFFEDWVAA SNRSTRATSSNKSDIVELMKKAETIK
Sbjct: 241 FPTHGTSMSRDNKLISGDNKGAAARFFEDWVAA-SNRSTRATSSNKSDIVELMKKAETIK 300

Query: 301 ATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSC 360
           ATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSC
Sbjct: 301 ATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSC 360

Query: 361 HGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIE 420
           HGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIE
Sbjct: 361 HGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIE 420

Query: 421 VELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVG 480
           VELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVG
Sbjct: 421 VELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVG 480

Query: 481 CQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETL 540
           CQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETL
Sbjct: 481 CQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETL 540

Query: 541 ISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTLSAYIQEFKKL 600
           ISNGNGEFDDDTHVVIAGLTNAYSQYVAT EEYEQQRYEAASTLYGPHTLSAYIQEFKKL
Sbjct: 541 ISNGNGEFDDDTHVVIAGLTNAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKL 600

Query: 601 AIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRG 660
           AIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRG
Sbjct: 601 AIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRG 660

Query: 661 SKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFTKS 694
           SKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFT S
Sbjct: 661 SKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFTNS 720

BLAST of Cp4.1LG11g08010 vs. NCBI nr
Match: KAG6599114.1 (Neutral ceramidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1338 bits (3463), Expect = 0.0
Identity = 685/766 (89.43%), Postives = 689/766 (89.95%), Query Frame = 0

Query: 1   MRRQLLKVVATAVLAAVSCCCA-DGDWLVGVGSFDMTGPAAEVNMMG------------- 60
           MRRQLLKVVATAVLAAVSCCCA DGDWLVGVGSFDMTGPAAEVNMMG             
Sbjct: 1   MRRQLLKVVATAVLAAVSCCCAADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHF 60

Query: 61  ----------------------------------------------------------FG 120
                                                                     FG
Sbjct: 61  RLRARTFIVAKSVDGPRIAFVNLDAGMASQLVTIKLLERLKASLTYLSKLKPRHFIQRFG 120

Query: 121 NLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESLKP 180
           NLY EEN+AISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAH SLKP
Sbjct: 121 NLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKP 180

Query: 181 GFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAFSW 240
           GF+LINKGEVENAGINRSPSAYLMNPPEERARYPTNVDT+MSLVKVVEGGSGVSLGAFSW
Sbjct: 181 GFILINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSW 240

Query: 241 FPTHGTSMSRDNKLISGDNKGAAARFFEDWVAAASNRSTRATSSNKSDIVELMKKAETIK 300
           FPTHGTSMSRDNKLISGDNKGAAARFFEDWVAA SNRSTRATSSNKSDI+ELMKKAETIK
Sbjct: 241 FPTHGTSMSRDNKLISGDNKGAAARFFEDWVAA-SNRSTRATSSNKSDILELMKKAETIK 300

Query: 301 ATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSC 360
           ATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSC
Sbjct: 301 ATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSC 360

Query: 361 HGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIE 420
           HGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIE
Sbjct: 361 HGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIE 420

Query: 421 VELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVG 480
           VELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVG
Sbjct: 421 VELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVG 480

Query: 481 CQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETL 540
           CQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETL
Sbjct: 481 CQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETL 540

Query: 541 ISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTLSAYIQEFKKL 600
           ISNGNGEFDDDTHVVIAGLTNAYSQYVAT EEYEQQRYEAASTLYGPHTLSAYIQEFKKL
Sbjct: 541 ISNGNGEFDDDTHVVIAGLTNAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKL 600

Query: 601 AIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRG 660
           AIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRG
Sbjct: 601 AIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRG 660

Query: 661 SKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFTKS 694
           SKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFT S
Sbjct: 661 SKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFTNS 720

BLAST of Cp4.1LG11g08010 vs. NCBI nr
Match: XP_038885883.1 (neutral ceramidase 2-like [Benincasa hispida])

HSP 1 Score: 1203 bits (3113), Expect = 0.0
Identity = 609/738 (82.52%), Postives = 636/738 (86.18%), Query Frame = 0

Query: 13  VLAAVSCCCADGDWLVGVGSFDMTGPAAEVNMMG-------------------------- 72
           V+AAVS C  +GDWLVGVGSFDMTGPAA VNM+G                          
Sbjct: 4   VIAAVSWC-VNGDWLVGVGSFDMTGPAAGVNMIGYANMDQNTAGIHFRLRARTFVVAKTV 63

Query: 73  ------------------------------FGNLYREENIAISGIHTHAGPGGYLQYLVY 132
                                         FG+LY EEN+AISGIHTHAGPGGYLQYLVY
Sbjct: 64  DGPRIAFINLDAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVY 123

Query: 133 SITSLGFVQQSFDAIVNAIEQSIVQAHESLKPGFMLINKGEVENAGINRSPSAYLMNPPE 192
           SITSLGFVQQSFDAIVNAIEQSI+QAHESLKPGF+L NKG+VEN GINRSPSAYLMNP E
Sbjct: 124 SITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKGDVENGGINRSPSAYLMNPEE 183

Query: 193 ERARYPTNVDTDMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFE 252
           ERARY  NVD +MS++K V+G SG S+GAFSWFPTHGTSMSRDNKLISGDNKGAAARFFE
Sbjct: 184 ERARYANNVDKEMSVLKFVDGESGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFE 243

Query: 253 DWVAAASNRSTRATSSNKSDIVELMKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGA 312
           DWVA  S R+   +S+N S IVELM+KAETIKATGGKRCSKTSSQ+SKVRKNDGSLFV A
Sbjct: 244 DWVA--SKRTKITSSNNTSGIVELMRKAETIKATGGKRCSKTSSQASKVRKNDGSLFVAA 303

Query: 313 FCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQ 372
           FCQSNVGDVTPNVLGAFC DSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQ
Sbjct: 304 FCQSNVGDVTPNVLGAFCIDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQ 363

Query: 373 FLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVELGGDNVVKTCPAALGPGFAAGTTDGP 432
           F KA DLFTTATE+LTGEIDFRHVYLNFTDIEVEL G+NVVKTCPAALGPGFAAGTTDGP
Sbjct: 364 FQKAADLFTTATEKLTGEIDFRHVYLNFTDIEVELDGNNVVKTCPAALGPGFAAGTTDGP 423

Query: 433 GVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQ 492
           GVFGFQQGDT+INKLWKKLRDSL+KPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQ
Sbjct: 424 GVFGFQQGDTEINKLWKKLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQ 483

Query: 493 ILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVA 552
           ILRLGKLIILSVPGEFTTMAGR+LREAVKETLI NGNGEFDDDTHVVIAGLTN YSQYVA
Sbjct: 484 ILRLGKLIILSVPGEFTTMAGRQLREAVKETLIRNGNGEFDDDTHVVIAGLTNTYSQYVA 543

Query: 553 TLEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFV 612
           T EEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAK E IAKPI SPPDLSSVQLRFV
Sbjct: 544 TFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKHENIAKPIASPPDLSSVQLRFV 603

Query: 613 SDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVE 672
            DPF ESPPNG+NFGDIQQDIKLP  GWFKRG KQKP A FWSANPRFDLLTEGTYAVVE
Sbjct: 604 IDPFGESPPNGINFGDIQQDIKLPTNGWFKRGGKQKPAATFWSANPRFDLLTEGTYAVVE 663

Query: 673 RLEKRRWTPVYDDDDFSLVFKWALDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKSA 694
           RLEK+RWTPVYDDDDFSL FKW LDNNTF  S+ATIEWDIPIDA+PGVYRLRHFGSSKS 
Sbjct: 664 RLEKQRWTPVYDDDDFSLFFKWTLDNNTFINSIATIEWDIPIDADPGVYRLRHFGSSKST 723

BLAST of Cp4.1LG11g08010 vs. NCBI nr
Match: XP_008464893.1 (PREDICTED: neutral ceramidase-like [Cucumis melo])

HSP 1 Score: 1191 bits (3081), Expect = 0.0
Identity = 598/739 (80.92%), Postives = 632/739 (85.52%), Query Frame = 0

Query: 21  CADGDWLVGVGSFDMTGPAAEVNMMG---------------------------------- 80
           C +GDWLVGVGSFDMTGPAAEVNMMG                                  
Sbjct: 11  CVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQVTAGIHFRLRARTFVVARSIDGPRIAFV 70

Query: 81  -------------------------------FGNLYREENIAISGIHTHAGPGGYLQYLV 140
                                          FG+LY EEN+AISGIHTHAGPGGYLQYLV
Sbjct: 71  NLDAGMASQLVTIKVLERLQSRRNINISVQRFGDLYTEENVAISGIHTHAGPGGYLQYLV 130

Query: 141 YSITSLGFVQQSFDAIVNAIEQSIVQAHESLKPGFMLINKGEVENAGINRSPSAYLMNPP 200
           YSITSLGFVQQSFDAIVNAIEQSI+QAHESLKPGF+L NKGEV+NAGINRSPSAYLMNP 
Sbjct: 131 YSITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKGEVDNAGINRSPSAYLMNPE 190

Query: 201 EERARYPTNVDTDMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARFF 260
           EERA+Y  NVD +MS++K V+G SG S+GAFSWFPTHGTSMSRDNKLISGDNKGAAARFF
Sbjct: 191 EERAKYANNVDKEMSILKFVDGESGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFF 250

Query: 261 EDWVAAASNRSTRATSSNKSDIVELMKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVG 320
           EDW AA++ R+T   S+N S I ELMKKAE IKATGGK+CSKTSS+SSKVRKNDGSLFV 
Sbjct: 251 EDW-AASNGRTTTTPSNNASVIEELMKKAEKIKATGGKQCSKTSSKSSKVRKNDGSLFVA 310

Query: 321 AFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGER 380
           AFCQSNVGDVTPNVLGAFC DSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGER
Sbjct: 311 AFCQSNVGDVTPNVLGAFCIDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGER 370

Query: 381 QFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVELGGDNVVKTCPAALGPGFAAGTTDG 440
           QFLKA DLFTTATE+LTGEIDFRHVYLNFTDIEVE+ G+NVV+TCPAALGPGFAAGTTDG
Sbjct: 371 QFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEIDGNNVVRTCPAALGPGFAAGTTDG 430

Query: 441 PGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPI 500
           PGVFGFQQGDT+INKLWK+LRDSL+KPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPI
Sbjct: 431 PGVFGFQQGDTEINKLWKQLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPI 490

Query: 501 QILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYV 560
           QILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTN YSQYV
Sbjct: 491 QILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYV 550

Query: 561 ATLEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRF 620
           AT EEYEQQRYEAASTLYGPHTLSAYIQEFKKLA AIAKGEK+AKPI SPPDLSSVQLR 
Sbjct: 551 ATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLASAIAKGEKVAKPIASPPDLSSVQLRL 610

Query: 621 VSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVV 680
           V DPF ESPP+GVNFGDIQQD++LPK GWFK GSKQKPTA FWSANPRFDLLTEGTYA+V
Sbjct: 611 VMDPFGESPPDGVNFGDIQQDVELPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALV 670

Query: 681 ERLEKRRWTPVYDDDDFSLVFKWALDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKS 694
           ERLEK+RWTP YDDDDFS+ FKW LDN T   SLATIEWDIPIDA+PGVYRLRHFGSS+S
Sbjct: 671 ERLEKQRWTPAYDDDDFSVSFKWKLDNTTLINSLATIEWDIPIDADPGVYRLRHFGSSRS 730

BLAST of Cp4.1LG11g08010 vs. NCBI nr
Match: XP_031742885.1 (neutral ceramidase 2 [Cucumis sativus])

HSP 1 Score: 1183 bits (3061), Expect = 0.0
Identity = 598/743 (80.48%), Postives = 633/743 (85.20%), Query Frame = 0

Query: 13  VLAAVSCCCADGDWLVGVGSFDMTGPAAEVNMMG-------------------------- 72
           V+ AVS C  +GDWLVGVGSFDMTGPAAEVNMMG                          
Sbjct: 4   VVVAVSWC-VNGDWLVGVGSFDMTGPAAEVNMMGYANMDQNTAGIHFRLRARTFVVASSV 63

Query: 73  -----------------------------------FGNLYREENIAISGIHTHAGPGGYL 132
                                              FG++Y EEN+AISGIHTHAGPGGYL
Sbjct: 64  DGPRIAFVNLDAGMASQLVTIKVLERLKSSILVQRFGDVYTEENVAISGIHTHAGPGGYL 123

Query: 133 QYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESLKPGFMLINKGEVENAGINRSPSAYL 192
           QYLVYSITSLGFVQQSFDAIVNAIEQSI+QAHESLKPGF+L NKGEVENAGINRSPSAYL
Sbjct: 124 QYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKGEVENAGINRSPSAYL 183

Query: 193 MNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKGAA 252
           MNP EERA+Y  NVD +MS++K V+G +G S+GAFSWFPTHGTSMSRDNKLISGDNKGAA
Sbjct: 184 MNPEEERAKYANNVDKEMSILKFVDGENGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAA 243

Query: 253 ARFFEDWVAAASNRSTRATSSNKSDIVELMKKAETIKATGGKRCSKTSSQSSKVRKNDGS 312
           ARFFEDW AA++ R+T   S+N S I +LMKKA  IKATGGKRCSKTSS+SSKVRKNDGS
Sbjct: 244 ARFFEDW-AASNRRTTTTPSNNTSGIEDLMKKAGKIKATGGKRCSKTSSKSSKVRKNDGS 303

Query: 313 LFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKI 372
           LFVGAFCQSNVGDVTPNVLGAFCTDSG PCDFN SSCHGSDLLCVGRGPGFPDEILSTKI
Sbjct: 304 LFVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDLLCVGRGPGFPDEILSTKI 363

Query: 373 IGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVELGGDNVVKTCPAALGPGFAAG 432
           IGERQFLKA DLFTTATE+LTGEIDFRHVYLNFTDIEV + G+NVV+TCPAALGPGFAAG
Sbjct: 364 IGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVAIDGNNVVRTCPAALGPGFAAG 423

Query: 433 TTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPA 492
           TTDGPG FGFQQGDT+INKLWK+LRDSL+KPSEFQVGCQKPKTVLLDTGEMFEPYAWAPA
Sbjct: 424 TTDGPGAFGFQQGDTEINKLWKQLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPA 483

Query: 493 ILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAY 552
           ILPIQILRLG LIILSVPGEFTTMAGRRLREAVKETLISNGNG FDDDTHVVIAGLTN Y
Sbjct: 484 ILPIQILRLGNLIILSVPGEFTTMAGRRLREAVKETLISNGNGVFDDDTHVVIAGLTNTY 543

Query: 553 SQYVATLEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSV 612
           SQYVAT EEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPI SPPDLSSV
Sbjct: 544 SQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIASPPDLSSV 603

Query: 613 QLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGT 672
           QLR V DPF ESPPNGVNFGDIQQD++LPK GWFK GSKQKPTA FWSANPRFDLLTEGT
Sbjct: 604 QLRLVMDPFGESPPNGVNFGDIQQDVELPKDGWFKTGSKQKPTATFWSANPRFDLLTEGT 663

Query: 673 YAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFTKSLATIEWDIPIDANPGVYRLRHFG 694
           YA+VERLEK+RWTP YDDDDFSL FKW LDN TF  +L TIEWDIPIDA+PGVYRLRHFG
Sbjct: 664 YALVERLEKQRWTPAYDDDDFSLFFKWKLDN-TFINNLVTIEWDIPIDADPGVYRLRHFG 723

BLAST of Cp4.1LG11g08010 vs. ExPASy TrEMBL
Match: A0A1S3CMI6 (Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103502652 PE=3 SV=1)

HSP 1 Score: 1191 bits (3081), Expect = 0.0
Identity = 598/739 (80.92%), Postives = 632/739 (85.52%), Query Frame = 0

Query: 21  CADGDWLVGVGSFDMTGPAAEVNMMG---------------------------------- 80
           C +GDWLVGVGSFDMTGPAAEVNMMG                                  
Sbjct: 11  CVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQVTAGIHFRLRARTFVVARSIDGPRIAFV 70

Query: 81  -------------------------------FGNLYREENIAISGIHTHAGPGGYLQYLV 140
                                          FG+LY EEN+AISGIHTHAGPGGYLQYLV
Sbjct: 71  NLDAGMASQLVTIKVLERLQSRRNINISVQRFGDLYTEENVAISGIHTHAGPGGYLQYLV 130

Query: 141 YSITSLGFVQQSFDAIVNAIEQSIVQAHESLKPGFMLINKGEVENAGINRSPSAYLMNPP 200
           YSITSLGFVQQSFDAIVNAIEQSI+QAHESLKPGF+L NKGEV+NAGINRSPSAYLMNP 
Sbjct: 131 YSITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKGEVDNAGINRSPSAYLMNPE 190

Query: 201 EERARYPTNVDTDMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARFF 260
           EERA+Y  NVD +MS++K V+G SG S+GAFSWFPTHGTSMSRDNKLISGDNKGAAARFF
Sbjct: 191 EERAKYANNVDKEMSILKFVDGESGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFF 250

Query: 261 EDWVAAASNRSTRATSSNKSDIVELMKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVG 320
           EDW AA++ R+T   S+N S I ELMKKAE IKATGGK+CSKTSS+SSKVRKNDGSLFV 
Sbjct: 251 EDW-AASNGRTTTTPSNNASVIEELMKKAEKIKATGGKQCSKTSSKSSKVRKNDGSLFVA 310

Query: 321 AFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGER 380
           AFCQSNVGDVTPNVLGAFC DSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGER
Sbjct: 311 AFCQSNVGDVTPNVLGAFCIDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGER 370

Query: 381 QFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVELGGDNVVKTCPAALGPGFAAGTTDG 440
           QFLKA DLFTTATE+LTGEIDFRHVYLNFTDIEVE+ G+NVV+TCPAALGPGFAAGTTDG
Sbjct: 371 QFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEIDGNNVVRTCPAALGPGFAAGTTDG 430

Query: 441 PGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPI 500
           PGVFGFQQGDT+INKLWK+LRDSL+KPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPI
Sbjct: 431 PGVFGFQQGDTEINKLWKQLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPI 490

Query: 501 QILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYV 560
           QILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTN YSQYV
Sbjct: 491 QILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYV 550

Query: 561 ATLEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRF 620
           AT EEYEQQRYEAASTLYGPHTLSAYIQEFKKLA AIAKGEK+AKPI SPPDLSSVQLR 
Sbjct: 551 ATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLASAIAKGEKVAKPIASPPDLSSVQLRL 610

Query: 621 VSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVV 680
           V DPF ESPP+GVNFGDIQQD++LPK GWFK GSKQKPTA FWSANPRFDLLTEGTYA+V
Sbjct: 611 VMDPFGESPPDGVNFGDIQQDVELPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALV 670

Query: 681 ERLEKRRWTPVYDDDDFSLVFKWALDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKS 694
           ERLEK+RWTP YDDDDFS+ FKW LDN T   SLATIEWDIPIDA+PGVYRLRHFGSS+S
Sbjct: 671 ERLEKQRWTPAYDDDDFSVSFKWKLDNTTLINSLATIEWDIPIDADPGVYRLRHFGSSRS 730

BLAST of Cp4.1LG11g08010 vs. ExPASy TrEMBL
Match: A0A0A0KII3 (Neutral ceramidase OS=Cucumis sativus OX=3659 GN=Csa_6G361230 PE=3 SV=1)

HSP 1 Score: 1152 bits (2981), Expect = 0.0
Identity = 571/661 (86.38%), Postives = 604/661 (91.38%), Query Frame = 0

Query: 44  MMGFGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAH 103
           M  FG++Y EEN+AISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSI+QAH
Sbjct: 1   MKTFGDVYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAH 60

Query: 104 ESLKPGFMLINKG----------EVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVK 163
           ESLKPGF+L NKG          EVENAGINRSPSAYLMNP EERA+Y  NVD +MS++K
Sbjct: 61  ESLKPGFILFNKGKQIRQLFHFWEVENAGINRSPSAYLMNPEEERAKYANNVDKEMSILK 120

Query: 164 VVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAAASNRSTRATSSN 223
            V+G +G S+GAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDW AA++ R+T   S+N
Sbjct: 121 FVDGENGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDW-AASNRRTTTTPSNN 180

Query: 224 KSDIVELMKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAF 283
            S I +LMKKA  IKATGGKRCSKTSS+SSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAF
Sbjct: 181 TSGIEDLMKKAGKIKATGGKRCSKTSSKSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAF 240

Query: 284 CTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTG 343
           CTDSG PCDFN SSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKA DLFTTATE+LTG
Sbjct: 241 CTDSGNPCDFNHSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTG 300

Query: 344 EIDFRHVYLNFTDIEVELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWK 403
           EIDFRHVYLNFTDIEV + G+NVV+TCPAALGPGFAAGTTDGPG FGFQQGDT+INKLWK
Sbjct: 301 EIDFRHVYLNFTDIEVAIDGNNVVRTCPAALGPGFAAGTTDGPGAFGFQQGDTEINKLWK 360

Query: 404 KLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFT 463
           +LRDSL+KPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLG LIILSVPGEFT
Sbjct: 361 QLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGNLIILSVPGEFT 420

Query: 464 TMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLY 523
           TMAGRRLREAVKETLISNGNG FDDDTHVVIAGLTN YSQYVAT EEYEQQRYEAASTLY
Sbjct: 421 TMAGRRLREAVKETLISNGNGVFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLY 480

Query: 524 GPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDI 583
           GPHTLSAYIQEFKKLAIAIAKGEKIAKPI SPPDLSSVQLR V DPF ESPPNGVNFGDI
Sbjct: 481 GPHTLSAYIQEFKKLAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGESPPNGVNFGDI 540

Query: 584 QQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFS 643
           QQD++LPK GWFK GSKQKPTA FWSANPRFDLLTEGTYA+VERLEK+RWTP YDDDDFS
Sbjct: 541 QQDVELPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFS 600

Query: 644 LVFKWALDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAV 694
           L FKW LDN TF  +L TIEWDIPIDA+PGVYRLRHFGSS+S INSTN+YFTGAS AFAV
Sbjct: 601 LFFKWKLDN-TFINNLVTIEWDIPIDADPGVYRLRHFGSSRSTINSTNVYFTGASRAFAV 659

BLAST of Cp4.1LG11g08010 vs. ExPASy TrEMBL
Match: A0A5A7TA71 (Neutral ceramidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold119G00340 PE=3 SV=1)

HSP 1 Score: 1060 bits (2742), Expect = 0.0
Identity = 545/731 (74.56%), Postives = 584/731 (79.89%), Query Frame = 0

Query: 6   LKVVATAVLAAVSCCCADGDWLVGVGSFDMTGPAAEVNMMGFGNLYREENIAISGIHTHA 65
           LK +A A++      C +GDWLVGVGSFDMTGPAAEVNMMG+ N+               
Sbjct: 10  LKFMAMAIVVLDVSWCVNGDWLVGVGSFDMTGPAAEVNMMGYANM--------------- 69

Query: 66  GPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESLKPGFMLINKGEVENAGINR 125
                                         +Q     H  L+    ++ + EV+NAGINR
Sbjct: 70  ------------------------------DQVTAGIHFRLRARTFVVARREVDNAGINR 129

Query: 126 SPSAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISG 185
           SPSAYLMNP EERA+Y  NVD +MS++K V+G SG S+GAFSWFPTHGTSMSRDNKLISG
Sbjct: 130 SPSAYLMNPEEERAKYANNVDKEMSILKFVDGESGNSIGAFSWFPTHGTSMSRDNKLISG 189

Query: 186 DNKGAAARFFEDWVAAASNRSTRATSSNKSD---------------IVELMKKAETIKAT 245
           DNKGAAARFFEDW AA++ R+T   S+N S+               I ELMKKAE IKAT
Sbjct: 190 DNKGAAARFFEDW-AASNGRTTTTPSNNASENLYNFLFLVYILKTVIEELMKKAEKIKAT 249

Query: 246 GGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHG 305
           GGKRCSKTSS+SSKVRKNDGSLFV AFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHG
Sbjct: 250 GGKRCSKTSSKSSKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHG 309

Query: 306 SDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVE 365
           SDLLCVGRGPGFPDEILSTKIIGERQFLKA DLFTTATE+LTGEIDFRHVYLNFTDIEVE
Sbjct: 310 SDLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVE 369

Query: 366 LGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDT------------------------ 425
           + G+NVV+TCPAALGPGFAAGTTDGPGVFGFQQGDT                        
Sbjct: 370 IDGNNVVRTCPAALGPGFAAGTTDGPGVFGFQQGDTEVSSMADGMNLCLSFFDCLNFGFA 429

Query: 426 ---QINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKL 485
              QINKLWK+LRDSL+KPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKL
Sbjct: 430 ILLQINKLWKQLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKL 489

Query: 486 IILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQ 545
           IILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTN YSQYVAT EEYEQ
Sbjct: 490 IILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQ 549

Query: 546 QRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIES 605
           QRYEAASTLYGPHTLSAYIQEFKKLA AIAKGEK+AKPI SPPDLSSVQLR V DPF ES
Sbjct: 550 QRYEAASTLYGPHTLSAYIQEFKKLASAIAKGEKVAKPIASPPDLSSVQLRLVMDPFGES 609

Query: 606 PPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRW 665
           PP+GVNFGDIQQD++LPK GWFK GSKQKPTA FWSANPRFDLLTEGTYA+VERLEK+RW
Sbjct: 610 PPDGVNFGDIQQDVELPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRW 669

Query: 666 TPVYDDDDFSLVFKWALDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIY 694
           TP YDDDDFS+ FKW LDN T   SLATIEWDIPIDA+PGVYRLRHFGSS+S INSTNIY
Sbjct: 670 TPAYDDDDFSVSFKWKLDNTTLINSLATIEWDIPIDADPGVYRLRHFGSSRSTINSTNIY 694

BLAST of Cp4.1LG11g08010 vs. ExPASy TrEMBL
Match: A0A2I4G350 (Neutral ceramidase OS=Juglans regia OX=51240 GN=LOC109004273 PE=3 SV=1)

HSP 1 Score: 1028 bits (2657), Expect = 0.0
Identity = 518/727 (71.25%), Postives = 588/727 (80.88%), Query Frame = 0

Query: 23  DGDWLVGVGSFDMTGPAAEVNMMG------------------------------------ 82
           +G++L GVGSFDMTGPAA VNMMG                                    
Sbjct: 26  NGEYLTGVGSFDMTGPAAGVNMMGYANIFQNTAGVHFRLRTRTFIVAESPQGPRFAFVNL 85

Query: 83  --------------------FGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQ 142
                               FGNLY +EN+AISGIHTHAGPGGYLQYL+YSITS GFVQQ
Sbjct: 86  DAGMASQLVTTKVLERLKSRFGNLYTQENVAISGIHTHAGPGGYLQYLLYSITSAGFVQQ 145

Query: 143 SFDAIVNAIEQSIVQAHESLKPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVD 202
           SFDAIV AIEQSIVQAH +LKPG + INKGEVENAGINRSPSAYL NP EER RYP NVD
Sbjct: 146 SFDAIVGAIEQSIVQAHNNLKPGSVFINKGEVENAGINRSPSAYLFNPAEERDRYPNNVD 205

Query: 203 TDMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAAASNRS 262
           T M+L+K+++ GSG S+GAFSW+ THGTSMSRDNKLISGDNKGAAARFFEDW +   N+S
Sbjct: 206 TQMTLLKLLDRGSGKSIGAFSWYATHGTSMSRDNKLISGDNKGAAARFFEDWFSF--NKS 265

Query: 263 TRATSSNKSDIVELMKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVT 322
           +    S+ ++I  L++KA+TIKATGG+ C KT+SQ+ KVRKNDGSLFVGAFCQSNVGDVT
Sbjct: 266 SSTPISSPAEIETLVEKAQTIKATGGQPCGKTTSQAFKVRKNDGSLFVGAFCQSNVGDVT 325

Query: 323 PNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTT 382
           PNVLGAFCTDSGKPCDFNRSSCHG+DLLCVGRGPG+PDEI STKIIGERQF KAVDLF T
Sbjct: 326 PNVLGAFCTDSGKPCDFNRSSCHGNDLLCVGRGPGYPDEIQSTKIIGERQFQKAVDLFAT 385

Query: 383 ATERLTGEIDFRHVYLNFTDIEVELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDT 442
           ATE LTG+ID+RHVYLNFT+I+VEL G  VVKTCPAA+GPGFAAGTTDGPGVFGFQQGDT
Sbjct: 386 ATEELTGKIDYRHVYLNFTNIDVELDGSKVVKTCPAAVGPGFAAGTTDGPGVFGFQQGDT 445

Query: 443 QINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIIL 502
           +I+++WKKLR++L++PS +QV CQKPKTVLLD GEMF+PYAWAPAILPIQ+LRLGKLIIL
Sbjct: 446 EISEMWKKLRNALKEPSPYQVECQKPKTVLLDAGEMFDPYAWAPAILPIQMLRLGKLIIL 505

Query: 503 SVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRY 562
           SVPGEFTTMAGRRLREAVKETLISNGNGEFDD T VVIAGLTN YSQYVAT EEYEQQRY
Sbjct: 506 SVPGEFTTMAGRRLREAVKETLISNGNGEFDDKTEVVIAGLTNTYSQYVATFEEYEQQRY 565

Query: 563 EAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPN 622
           EAASTLYGPHTLSAYIQEF KLA A+AKGEK+     +PPDLSSVQL+F+ +P  +SPP 
Sbjct: 566 EAASTLYGPHTLSAYIQEFNKLAKAMAKGEKLISNGPTPPDLSSVQLKFLLEPVGDSPPP 625

Query: 623 GVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPV 682
           G NFGD++QDI +PK G F++G K  P+A FWSANPR+DLLTEGT+AVVE L+  RW PV
Sbjct: 626 GKNFGDMKQDIIIPKSGTFRKGDK--PSATFWSANPRYDLLTEGTFAVVEMLQVERWIPV 685

Query: 683 YDDDDFSLVFKWALDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTG 693
           +DDDDF L FKW++DN++F   LATIEW+IP   + GVYRLRHFGS+K    S N YFTG
Sbjct: 686 FDDDDFCLYFKWSVDNSSF-YGLATIEWEIPEVVSSGVYRLRHFGSAKKTKASPNTYFTG 745

BLAST of Cp4.1LG11g08010 vs. ExPASy TrEMBL
Match: A0A2H5PA29 (Neutral ceramidase OS=Citrus unshiu OX=55188 GN=CUMW_117460 PE=3 SV=1)

HSP 1 Score: 1025 bits (2651), Expect = 0.0
Identity = 519/732 (70.90%), Postives = 588/732 (80.33%), Query Frame = 0

Query: 22  ADGDWLVGVGSFDMTGPAAEVNMMG----------------------------------- 81
           ADG +LVGVGS+DMTGPAA +NMMG                                   
Sbjct: 113 ADGQYLVGVGSYDMTGPAAGINMMGYANLFQNTAGIHFRLRARTFIVAESSNGTRFAFVN 172

Query: 82  ---------------------FGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQ 141
                                FGNLY EENIAISGIHTHAGPGGYLQYL+YSITSLGFVQ
Sbjct: 173 LDAGMASQLVTIKVLEKLKTRFGNLYTEENIAISGIHTHAGPGGYLQYLIYSITSLGFVQ 232

Query: 142 QSFDAIVNAIEQSIVQAHESLKPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNV 201
           QSFDAIV+AI QSIVQAH +LKPG + I KG+VENAGINRSPSAYL+NP EERARYP NV
Sbjct: 233 QSFDAIVSAIVQSIVQAHNNLKPGSIFIRKGDVENAGINRSPSAYLLNPAEERARYPENV 292

Query: 202 DTDMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAAASNR 261
           D +M+L+K V+  SG S+GAF+W+ THGTSMSRDN LISGDNKGAAARFFEDW ++ +  
Sbjct: 293 DKEMTLLKFVDAASGKSIGAFNWYATHGTSMSRDNLLISGDNKGAAARFFEDWFSSKNES 352

Query: 262 STRATS--SNKSDIVELMKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVG 321
           S+  TS  S  SDI +LM+K++TIKATGG++C KT+SQ  KVRKNDGSLFVGAFCQSNVG
Sbjct: 353 SSITTSKFSTSSDIGKLMEKSKTIKATGGQQCGKTTSQGFKVRKNDGSLFVGAFCQSNVG 412

Query: 322 DVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDL 381
           DVTPNVLGAFC DSGKPCDFN SSCHG D LC+GRGPG+PDEILSTKIIGERQF KAVDL
Sbjct: 413 DVTPNVLGAFCIDSGKPCDFNHSSCHGDDQLCIGRGPGYPDEILSTKIIGERQFQKAVDL 472

Query: 382 FTTATERLTGEIDFRHVYLNFTDIEVELGG--DNVVKTCPAALGPGFAAGTTDGPGVFGF 441
           F +AT++L+G+ID+RHVY+NFT+IEV L G  +N VKTCPAALGPGFAAGTTDGPGVFGF
Sbjct: 473 FNSATDQLSGKIDYRHVYINFTNIEVGLDGTGNNTVKTCPAALGPGFAAGTTDGPGVFGF 532

Query: 442 QQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLG 501
           QQGDT+INKLWK+LRD L+KPS++Q  CQKPK VLL +GEMFEPYAWAPA+LPIQILRLG
Sbjct: 533 QQGDTEINKLWKQLRDVLKKPSQYQEECQKPKAVLLSSGEMFEPYAWAPAVLPIQILRLG 592

Query: 502 KLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEY 561
           KLIILSVPGEFTTMAGRRLREAVK+TLI+NGNGEFD+DTH+VIAGLTNAYSQY+AT EEY
Sbjct: 593 KLIILSVPGEFTTMAGRRLREAVKDTLINNGNGEFDNDTHIVIAGLTNAYSQYIATFEEY 652

Query: 562 EQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFI 621
             QRYEAASTLYGPHTL AYIQEFKKLA A+AKGEK  K   SPPDLSSVQL    +P  
Sbjct: 653 AHQRYEAASTLYGPHTLPAYIQEFKKLAQAMAKGEKTTKG-PSPPDLSSVQLSLSLNPTG 712

Query: 622 ESPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLEKR 681
           +SPP G  FGDI++DI  PK G F +G +  P+A FWSANPR+DLLTEGTYAVVE L+ +
Sbjct: 713 DSPPAGKKFGDIKEDINQPKDGSFNKGDR--PSATFWSANPRYDLLTEGTYAVVEMLQGK 772

Query: 682 RWTPVYDDDDFSLVFKWALDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTN 693
           RW PVYDDDDFSL FKW+L+N +F   LAT+EW++P +A PGVYRLRHFGSSK A+NSTN
Sbjct: 773 RWIPVYDDDDFSLYFKWSLENTSF-HGLATVEWEVPTEAIPGVYRLRHFGSSKKAVNSTN 832

BLAST of Cp4.1LG11g08010 vs. TAIR 10
Match: AT2G38010.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 742.7 bits (1916), Expect = 2.8e-214
Identity = 383/662 (57.85%), Postives = 477/662 (72.05%), Query Frame = 0

Query: 47  FGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESL 106
           +G LY E+N+AISGIHTHAGPGGYLQY+ Y +TSLGFV+QSFD +VN IEQSIVQAHESL
Sbjct: 102 YGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFDVVVNGIEQSIVQAHESL 161

Query: 107 KPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAF 166
           +PG   +NKG++ +AG+NRSPS+YL NP  ER++Y  +VD +M+LVK V+   G + G+F
Sbjct: 162 RPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEMTLVKFVDSQLGPT-GSF 221

Query: 167 SWFPTHGTSMSRDNKLISGDNKGAAARFFEDWV------AAASNRSTRATSSNKSDIV-- 226
           +WF THGTSMSR N LISGDNKGAAARF EDW       + +S    R  S+  SD    
Sbjct: 222 NWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENGQKNSVSSRNIPRRVSTIVSDFSRN 281

Query: 227 --ELMKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTD 286
              L+  A T K++ G    K+    ++VR      FV AFCQSN GDV+PN LG FC D
Sbjct: 282 RDRLLDIAATYKSSRGHSVDKSLDVKTRVRNGSKRKFVSAFCQSNCGDVSPNTLGTFCID 341

Query: 287 SGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEID 346
           +G PCDFN S+C+G + LC GRGPG+PDE  ST+IIGE+QF  AV+LF  ATE+L G+I 
Sbjct: 342 TGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIG 401

Query: 347 FRHVYLNFTDIEVEL----GGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLW 406
           ++H YL+F++++V +    GG   VKTCPAA+G GFAAGTTDGPG F F+QGD Q N  W
Sbjct: 402 YQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFW 461

Query: 407 KKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEF 466
           + +R+ LR P   QV CQKPK +LLDTGEM EPY WAP+ILPIQILR+G+L+ILSVPGEF
Sbjct: 462 RLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEF 521

Query: 467 TTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTL 526
           TTMAGRRLR+A+K  LIS+   EF ++ HVVIAGLTN YSQY+AT EEYE QRYE ASTL
Sbjct: 522 TTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTL 581

Query: 527 YGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGD 586
           YG HTL+AYIQEFKKLA A+  G  + +    PPDL   Q+  +S   ++S P GV FGD
Sbjct: 582 YGRHTLTAYIQEFKKLATALVNGLTLPRG-PQPPDLLDKQISLLSPVVVDSTPLGVKFGD 641

Query: 587 IQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERL-EKRRWTPVYDDDD 646
           ++ D+  P    F+RG  Q+  A FWS  PR DL+TEG++AVVE L E  +W PVYDDDD
Sbjct: 642 VKADV--PPKSTFRRG--QQVNATFWSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDD 701

Query: 647 FSLVFKWALDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAF 694
           FSL FKW+      ++S ATIEW +P  A  GVYR+RH+G+SKS   S +  F+G+SSAF
Sbjct: 702 FSLKFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRHYGASKSLFGSIS-SFSGSSSAF 756

BLAST of Cp4.1LG11g08010 vs. TAIR 10
Match: AT2G38010.2 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 729.2 bits (1881), Expect = 3.2e-210
Identity = 383/697 (54.95%), Postives = 477/697 (68.44%), Query Frame = 0

Query: 47  FGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESL 106
           +G LY E+N+AISGIHTHAGPGGYLQY+ Y +TSLGFV+QSFD +VN IEQSIVQAHESL
Sbjct: 102 YGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFDVVVNGIEQSIVQAHESL 161

Query: 107 KPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAF 166
           +PG   +NKG++ +AG+NRSPS+YL NP  ER++Y  +VD +M+LVK V+   G + G+F
Sbjct: 162 RPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEMTLVKFVDSQLGPT-GSF 221

Query: 167 SWFPTHGTSMSRDNKLISGDNKGAAARFFEDWV------AAASNRSTRATSSNKSDI--- 226
           +WF THGTSMSR N LISGDNKGAAARF EDW       + +S    R  S+  SD    
Sbjct: 222 NWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENGQKNSVSSRNIPRRVSTIVSDFSRN 281

Query: 227 ------------------------------------VELMKKAETIKATGGKRCSKTSSQ 286
                                                 L+  A T K++ G    K+   
Sbjct: 282 RESYPFLELPNVAILYIAVAERLMWKVMVLFYFVSESRLLDIAATYKSSRGHSVDKSLDV 341

Query: 287 SSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPG 346
            ++VR      FV AFCQSN GDV+PN LG FC D+G PCDFN S+C+G + LC GRGPG
Sbjct: 342 KTRVRNGSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPG 401

Query: 347 FPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVEL----GGDNVV 406
           +PDE  ST+IIGE+QF  AV+LF  ATE+L G+I ++H YL+F++++V +    GG   V
Sbjct: 402 YPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETV 461

Query: 407 KTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLL 466
           KTCPAA+G GFAAGTTDGPG F F+QGD Q N  W+ +R+ LR P   QV CQKPK +LL
Sbjct: 462 KTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILL 521

Query: 467 DTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFD 526
           DTGEM EPY WAP+ILPIQILR+G+L+ILSVPGEFTTMAGRRLR+A+K  LIS+   EF 
Sbjct: 522 DTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFS 581

Query: 527 DDTHVVIAGLTNAYSQYVATLEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEK 586
           ++ HVVIAGLTN YSQY+AT EEYE QRYE ASTLYG HTL+AYIQEFKKLA A+  G  
Sbjct: 582 NNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLT 641

Query: 587 IAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARF 646
           + +    PPDL   Q+  +S   ++S P GV FGD++ D+  P    F+RG  Q+  A F
Sbjct: 642 LPRG-PQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKADV--PPKSTFRRG--QQVNATF 701

Query: 647 WSANPRFDLLTEGTYAVVERL-EKRRWTPVYDDDDFSLVFKWALDNNTFTKSLATIEWDI 694
           WS  PR DL+TEG++AVVE L E  +W PVYDDDDFSL FKW+      ++S ATIEW +
Sbjct: 702 WSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRV 761

BLAST of Cp4.1LG11g08010 vs. TAIR 10
Match: AT1G07380.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 718.8 bits (1854), Expect = 4.3e-207
Identity = 383/749 (51.13%), Postives = 499/749 (66.62%), Query Frame = 0

Query: 25  DWLVGVGSFDMTGPAAEVNMMG-------------------------------------- 84
           ++L+G+GS+D+TGPAA+VNMMG                                      
Sbjct: 36  EYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFIVSEPQGKRVVFVNLDAC 95

Query: 85  -----------------FGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFD 144
                            +G+LY E+N+ ISGIHTHAGPGGYLQY+VY +TSLGFV+QSFD
Sbjct: 96  MASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFD 155

Query: 145 AIVNAIEQSIVQAHESLKPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDM 204
           A+V+ IE SI+QAHE+L+PG + +N GE+ +AG+NRSPSAYL NP +ER+++  NVD +M
Sbjct: 156 ALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPSAYLNNPSKERSKHKYNVDKEM 215

Query: 205 SLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAA-------- 264
           +L+K V+   G  +G+F+WF THGTSMSR N LISGDNKGAA+RF EDW           
Sbjct: 216 TLLKFVDDQWG-PVGSFNWFATHGTSMSRTNSLISGDNKGAASRFMEDWYEQNTAERSYS 275

Query: 265 ---ASNRSTRATSS----NKSDIVELMKKAETIKATGGKRCSKTSSQSSKV----RKNDG 324
               S+   R  SS    ++    EL++ A   ++  GK  ++ SS + +V    RK D 
Sbjct: 276 EEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKPVTRISSSARRVRSALRKADK 335

Query: 325 SLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTK 384
             FV AFCQ+N GDV+PNVLGAFC D+G PCDFN S+C G + +C GRGPG+PDE  ST+
Sbjct: 336 PGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGGKNEMCYGRGPGYPDEFESTR 395

Query: 385 IIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVEL----GGDNVVKTCPAALGP 444
           IIGERQF  A++LF  A+E+L G++D+RHVY++F+ + V L    G   VVKTCPAA+G 
Sbjct: 396 IIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKKDGKSEVVKTCPAAMGF 455

Query: 445 GFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPY 504
            FAAGTTDGPG F F QGD + N  W+ +R+ L+ P + Q+ C  PK +LLDTGEM +PY
Sbjct: 456 AFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDCHYPKPILLDTGEMTKPY 515

Query: 505 AWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAG 564
            WAP+IL +Q+LR+G+L ILSVPGEFTTMAGRRLR AVK  L ++GN +   + HVVIAG
Sbjct: 516 DWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKTQLKNSGNKDLSGEIHVVIAG 575

Query: 565 LTNAYSQYVATLEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPP 624
           L N YSQYV T EEY+ QRYE ASTLYGPHTLS YIQEFKKL+ ++     + +P   PP
Sbjct: 576 LANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLSKSLVLDMPV-QPGPQPP 635

Query: 625 DLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDL 684
           DL   QL F++   +++ P+G +FGD+  D+  PK    KRG+ Q  T  F SA PR DL
Sbjct: 636 DLLDKQLSFLTPVMMDTTPSGDSFGDVISDV--PKNLSLKRGNGQ-VTVVFRSACPRNDL 695

Query: 685 LTEGTYAVVERLEK--RRWTPVYDDDDFSLVFKWALDNNTFTKSLATIEWDIPIDANPGV 694
           LTEGT+ +VERLE+  + WTPVYDDDD  L FKW+      ++S AT+EW IP  A+PGV
Sbjct: 696 LTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRSQATVEWRIPESASPGV 755

BLAST of Cp4.1LG11g08010 vs. TAIR 10
Match: AT5G58980.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 676.8 bits (1745), Expect = 1.9e-194
Identity = 360/666 (54.05%), Postives = 459/666 (68.92%), Query Frame = 0

Query: 47  FGNLYREENIAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHESL 106
           +G LY EEN+AISG HTHAGPGGYLQY++Y +TSLGFV QSF+A+V+ IEQSI+QAHE+L
Sbjct: 105 YGELYTEENVAISGTHTHAGPGGYLQYILYLVTSLGFVHQSFNALVDGIEQSIIQAHENL 164

Query: 107 KPGFMLINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTDMSLVKVVEGGSGVSLGAF 166
           +PG +LINKGE+ +AG+NRSPSAYL NP  ER++Y  +VD +M+LVK V+          
Sbjct: 165 RPGSILINKGELLDAGVNRSPSAYLNNPAHERSKYEYDVDKEMTLVKFVD---------- 224

Query: 167 SWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAAASN------RSTRATSSNKSDIV-- 226
                              D  G  AR  EDW    +        S R  SS  SD    
Sbjct: 225 -------------------DQWGPVARIMEDWFERENGCRSVDVESPRRVSSIISDPYDQ 284

Query: 227 ELMKKAETIKATGGKRCSKTSSQSSKVRKN----DGSLFVGAFCQSNVGDVTPNVLGAFC 286
           +LM+ A ++ +TGGK  ++ SS + +VR      D   FV AFCQ+N GDV+PNVLGAFC
Sbjct: 285 DLMEMASSLLSTGGKTVTRMSSVARRVRSRFRHADKPRFVSAFCQTNCGDVSPNVLGAFC 344

Query: 287 TDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGE 346
            D+G PC+FN+S+C G +  C GRGPG+PDE  ST+IIGERQF KA DLFT A+E + G+
Sbjct: 345 IDTGLPCEFNQSTCGGKNEQCYGRGPGYPDEFESTRIIGERQFKKAADLFTKASEEIQGK 404

Query: 347 IDFRHVYLNFTDIEVEL----GGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINK 406
           +D+RH Y++F+ +EV +    GG  VVKTCPAA+G GFAAGTTDGPG F F+QGD Q N 
Sbjct: 405 VDYRHAYVDFSQLEVTINGQNGGSEVVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNP 464

Query: 407 LWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPG 466
            W+ +R+ L+ P+E QV CQ+PK +LLDTGEM +PY WAP+ILP+QILR+G+L+IL VPG
Sbjct: 465 FWRLVRNLLKNPTEEQVRCQRPKPILLDTGEMKQPYDWAPSILPVQILRIGQLVILCVPG 524

Query: 467 EFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATLEEYEQQRYEAAS 526
           EFTTMAGRRLR+AVK  L    NG    +  VVIAGLTN+YSQY+AT EEY+ QRYE AS
Sbjct: 525 EFTTMAGRRLRDAVKTVLKEGSNGR---EFSVVIAGLTNSYSQYIATFEEYQVQRYEGAS 584

Query: 527 TLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNF 586
           TLYGPHTLS YIQEFKKLA  +   +    P   PPDL   Q+  ++    +  P G  F
Sbjct: 585 TLYGPHTLSGYIQEFKKLANDLLSAQ-TTDPGPQPPDLLHKQISLLTPVVADMTPIGTAF 644

Query: 587 GDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVER-LEKRR-WTPVYD 646
           GD+  D+  P++  F++G+      +F SANPR DL+TEGT+A+VER LE R  W PVYD
Sbjct: 645 GDVTSDV--PRLSKFRKGA-DIVRVQFRSANPRNDLMTEGTFALVERWLEGRETWVPVYD 704

Query: 647 DDDFSLVFKWALDNNTFTKSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGAS 695
           DDDF L FKW+      T+S ATIEW IP  A+PGVYR+ HFGS+K+ I+S + +F+G+S
Sbjct: 705 DDDFCLRFKWSRPFKLSTQSTATIEWRIPETASPGVYRITHFGSAKTPISSIH-HFSGSS 733

HSP 2 Score: 53.1 bits (126), Expect = 1.0e-06
Identity = 25/49 (51.02%), Postives = 38/49 (77.55%), Query Frame = 0

Query: 14 LAAVSCCCADGDWLVGVGSFDMTGPAAEVNMMGFGNLYREENIAISGIH 63
          L  ++C  +D D+L+G+GS+D+TGPAA+VNMMG+ N+   E +A SG+H
Sbjct: 17 LLRLTCIFSDSDYLMGLGSYDITGPAADVNMMGYANM---EQVA-SGVH 61